Bhi03G001123 (gene) Wax gourd

Overview
NameBhi03G001123
Typegene
OrganismBenincasa hispida (Wax gourd)
DescriptionSubtilisin-like serine protease
Locationchr3 : 30855184 .. 30855935 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAACAGCAAAGTGATCGGCGCAAGCTACTTCGATCTGGACAACGTAAGCCAATATCCAGAGTCGAGCGTGGCGGACACGGAGGGGCATGGTTCTCACACGGCGTCGACGGTGGCAGGATCGACGGTGGAGGGGGCGAGCTTGTACGGCCTGACCAAAGGAACGGCACGCGGCGGCGTGCCTTCAGCTCGAATTGCAGTCTACAAAGTGTGTTGGAATATTTTCTGCAGCGAAATGGACGTTCTTGCTGGCTTCGAGCAGGCCATCGCCGACGGCGTCGATTTGATTTCCGTCTCCATAGGCTCCCTGCCCATGGATTTCTTCAGGGACGGCCAAGCAATTGGGGCTTTCCATGCCATGAAGAAGGGAATTTTAACGTCCTGCGCCGCCGGCAACGACGGCCCTGAATTGTCCACCGTCGAAAATGTGGCGCCTTGGATTATGACCGTCGCTGCTACGGCCATTGATAGACGATTCGTCACCGCTTTTAAACTCGGTAATGGCAACAAATTTACCGTGAGTCTCTCTCCCTCTTTCTCTAAAATATTTTTTGTGTAATTTTTTTAAAATTGAATTTACTGAAAATATTTTCTCTTTATAAAATTACTTTATATGCTGTAAATTGAAGTAAATTATTTTTAGTTTACATTACTTATATTATATGAATCCACTACATTTTTTTAGTGAAGTAGTATTTACATTTTATTTTACTATAGGAATTAGAAGGCTTCGAACCATGAATGCAAGGTAA

mRNA sequence

ATGGAACAGCAAAGTGATCGGCGCAAGCTACTTCGATCTGGACAACGTAAGCCAATATCCAGAGGGCATGGTTCTCACACGGCGTCGACGGTGGCAGGATCGACGGTGGAGGGGGCGAGCTTGTACGGCCTGACCAAAGGAACGGCACGCGGCGGCGTGCCTTCAGCTCGAATTGCAGTCTACAAAGTGTGTTGGAATATTTTCTGCAGCGAAATGGACGTTCTTGCTGGCTTCGAGCAGGCCATCGCCGACGGCGTCGATTTGATTTCCGTCTCCATAGGCTCCCTGCCCATGGATTTCTTCAGGGACGGCCAAGCAATTGGGGCTTTCCATGCCATGAAGAAGGGAATTTTAACGTCCTGCGCCGCCGGCAACGACGGCCCTGAATTGTCCACCGTCGAAAATGTGGCGCCTTGGATTATGACCGTCGCTGCTACGGCCATTGATAGACGATTCGTCACCGCTTTTAAACTCGGAATTAGAAGGCTTCGAACCATGAATGCAAGGTAA

Coding sequence (CDS)

ATGGAACAGCAAAGTGATCGGCGCAAGCTACTTCGATCTGGACAACGTAAGCCAATATCCAGAGGGCATGGTTCTCACACGGCGTCGACGGTGGCAGGATCGACGGTGGAGGGGGCGAGCTTGTACGGCCTGACCAAAGGAACGGCACGCGGCGGCGTGCCTTCAGCTCGAATTGCAGTCTACAAAGTGTGTTGGAATATTTTCTGCAGCGAAATGGACGTTCTTGCTGGCTTCGAGCAGGCCATCGCCGACGGCGTCGATTTGATTTCCGTCTCCATAGGCTCCCTGCCCATGGATTTCTTCAGGGACGGCCAAGCAATTGGGGCTTTCCATGCCATGAAGAAGGGAATTTTAACGTCCTGCGCCGCCGGCAACGACGGCCCTGAATTGTCCACCGTCGAAAATGTGGCGCCTTGGATTATGACCGTCGCTGCTACGGCCATTGATAGACGATTCGTCACCGCTTTTAAACTCGGAATTAGAAGGCTTCGAACCATGAATGCAAGGTAA

Protein sequence

MEQQSDRRKLLRSGQRKPISRGHGSHTASTVAGSTVEGASLYGLTKGTARGGVPSARIAVYKVCWNIFCSEMDVLAGFEQAIADGVDLISVSIGSLPMDFFRDGQAIGAFHAMKKGILTSCAAGNDGPELSTVENVAPWIMTVAATAIDRRFVTAFKLGIRRLRTMNAR
Homology
[Close]
BLAST of Bhi03G001123 vs. Swiss-Prot
Match: sp|Q9LZS6|SBT4F_ARATH (Subtilisin-like protease SBT4.15 OS=Arabidopsis thaliana OX=3702 GN=SBT4.15 PE=3 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 2.5e-49
Identity = 90/138 (65.22%), Postives = 115/138 (83.33%), Query Frame = 0 

Query: 22  GHGSHTASTVAGSTVEGASLYGLTKGTARGGVPSARIAVYKVCWNIFCSEMDVLAGFEQA 81
           GHG+HT+ST+AG +V  ASL+G+  GTARGGVPSARIA YKVCW+  C++MD+LA F++A
Sbjct: 209 GHGTHTSSTIAGVSVSSASLFGIANGTARGGVPSARIAAYKVCWDSGCTDMDMLAAFDEA 268

Query: 82  IADGVDLISVSIGSLPMDFFRDGQAIGAFHAMKKGILTSCAAGNDGPELSTVENVAPWIM 141
           I+DGVD+IS+SIG   + FF D  AIGAFHAMK+GILT+C+AGN+GP L TV N+APW+M
Sbjct: 269 ISDGVDIISISIGGASLPFFEDPIAIGAFHAMKRGILTTCSAGNNGPGLFTVSNLAPWVM 328

Query: 142 TVAATAIDRRFVTAFKLG 160
           TVAA ++DR+F T  KLG
Sbjct: 329 TVAANSLDRKFETVVKLG 346

[Close]
BLAST of Bhi03G001123 vs. Swiss-Prot
Match: sp|Q9LLL8|SBT4E_ARATH (Subtilisin-like protease SBT4.14 OS=Arabidopsis thaliana OX=3702 GN=SBT4.14 PE=2 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 8.6e-42
Identity = 87/146 (59.59%), Postives = 107/146 (73.29%), Query Frame = 0 

Query: 16  RKPIS-RGHGSHTASTVAGSTVEGASLYGLTKGTARGGVPSARIAVYKVCW-NIFCSEMD 75
           R PI   GHG+HT+STVAG  V  ASLYG+  GTARG VPSAR+A+YKVCW    C++MD
Sbjct: 202 RSPIDIDGHGTHTSSTVAGVLVANASLYGIANGTARGAVPSARLAMYKVCWARSGCADMD 261

Query: 76  VLAGFEQAIADGVDLISVSIGSLPMDFFRDGQAIGAFHAMKKGILTSCAAGNDGPELSTV 135
           +LAGFE AI DGV++IS+SIG    D+  D  ++G+FHAM+KGILT  +AGNDGP   TV
Sbjct: 262 ILAGFEAAIHDGVEIISISIGGPIADYSSDSISVGSFHAMRKGILTVASAGNDGPSSGTV 321

Query: 136 ENVAPWIMTVAATAIDRRFVTAFKLG 160
            N  PWI+TVAA+ IDR F +   LG
Sbjct: 322 TNHEPWILTVAASGIDRTFKSKIDLG 347

[Close]
BLAST of Bhi03G001123 vs. Swiss-Prot
Match: sp|Q39547|CUCM1_CUCME (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 2.5e-41
Identity = 81/141 (57.45%), Postives = 108/141 (76.60%), Query Frame = 0 

Query: 20  SRGHGSHTASTVAGSTVEGASLYGLTKGTARGGVPSARIAVYKVCWNIFCSEMDVLAGFE 79
           + GHG+HTAST AG  V  A+LYGL  GTARGGVP ARIA YKVCWN  CS+ D+LA ++
Sbjct: 201 TNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIAAYKVCWNDGCSDTDILAAYD 260

Query: 80  QAIADGVDLISVSIGSL-PMDFFRDGQAIGAFHAMKKGILTSCAAGNDGPELSTVENVAP 139
            AIADGVD+IS+S+G   P  +F D  AIG+FHA+++GILTS +AGN GP   T  +++P
Sbjct: 261 DAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGILTSNSAGNGGPNFFTTASLSP 320

Query: 140 WIMTVAATAIDRRFVTAFKLG 160
           W+++VAA+ +DR+FVT  ++G
Sbjct: 321 WLLSVAASTMDRKFVTQVQIG 341

[Close]
BLAST of Bhi03G001123 vs. Swiss-Prot
Match: sp|O65351|SBT17_ARATH (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 6.8e-39
Identity = 80/138 (57.97%), Postives = 99/138 (71.74%), Query Frame = 0 

Query: 22  GHGSHTASTVAGSTVEGASLYGLTKGTARGGVPSARIAVYKVCWNIFCSEMDVLAGFEQA 81
           GHG+HT+ST AGS VEGASL G   GTARG  P AR+AVYKVCW   C   D+LA  ++A
Sbjct: 211 GHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWLGGCFSSDILAAIDKA 270

Query: 82  IADGVDLISVSIGSLPMDFFRDGQAIGAFHAMKKGILTSCAAGNDGPELSTVENVAPWIM 141
           IAD V+++S+S+G    D++RDG AIGAF AM++GIL SC+AGN GP  S++ NVAPWI 
Sbjct: 271 IADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWIT 330

Query: 142 TVAATAIDRRFVTAFKLG 160
           TV A  +DR F     LG
Sbjct: 331 TVGAGTLDRDFPALAILG 348

[Close]
BLAST of Bhi03G001123 vs. Swiss-Prot
Match: sp|Q9FIF8|SBT43_ARATH (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 1.5e-38
Identity = 79/139 (56.83%), Postives = 107/139 (76.98%), Query Frame = 0 

Query: 22  GHGSHTASTVAGSTVEGASLYGLTKGTARGGVPSARIAVYKVCWNIFCSEMDVLAGFEQA 81
           GHG+HTAST AG+ V+ AS YGL +GTARGGVPSARIA YKVC+N  C+++D+LA F+ A
Sbjct: 195 GHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCFN-RCNDVDILAAFDDA 254

Query: 82  IADGVDLISVSIGS-LPMDFFRDGQAIGAFHAMKKGILTSCAAGNDGPELSTVENVAPWI 141
           IADGVD+IS+SI +    +      AIG+FHAM +GI+T+ +AGN+GP+  +V NV+PW+
Sbjct: 255 IADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWM 314

Query: 142 MTVAATAIDRRFVTAFKLG 160
           +TVAA+  DR+F+    LG
Sbjct: 315 ITVAASGTDRQFIDRVVLG 332

[Close]
BLAST of Bhi03G001123 vs. TAIR10
Match: AT5G03620.1 (Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 196.4 bits (498), Expect = 1.4e-50
Identity = 90/138 (65.22%), Postives = 115/138 (83.33%), Query Frame = 0 

Query: 22  GHGSHTASTVAGSTVEGASLYGLTKGTARGGVPSARIAVYKVCWNIFCSEMDVLAGFEQA 81
           GHG+HT+ST+AG +V  ASL+G+  GTARGGVPSARIA YKVCW+  C++MD+LA F++A
Sbjct: 209 GHGTHTSSTIAGVSVSSASLFGIANGTARGGVPSARIAAYKVCWDSGCTDMDMLAAFDEA 268

Query: 82  IADGVDLISVSIGSLPMDFFRDGQAIGAFHAMKKGILTSCAAGNDGPELSTVENVAPWIM 141
           I+DGVD+IS+SIG   + FF D  AIGAFHAMK+GILT+C+AGN+GP L TV N+APW+M
Sbjct: 269 ISDGVDIISISIGGASLPFFEDPIAIGAFHAMKRGILTTCSAGNNGPGLFTVSNLAPWVM 328

Query: 142 TVAATAIDRRFVTAFKLG 160
           TVAA ++DR+F T  KLG
Sbjct: 329 TVAANSLDRKFETVVKLG 346

[Close]
BLAST of Bhi03G001123 vs. TAIR10
Match: AT4G00230.1 (xylem serine peptidase 1)

HSP 1 Score: 171.4 bits (433), Expect = 4.8e-43
Identity = 87/146 (59.59%), Postives = 107/146 (73.29%), Query Frame = 0 

Query: 16  RKPIS-RGHGSHTASTVAGSTVEGASLYGLTKGTARGGVPSARIAVYKVCW-NIFCSEMD 75
           R PI   GHG+HT+STVAG  V  ASLYG+  GTARG VPSAR+A+YKVCW    C++MD
Sbjct: 202 RSPIDIDGHGTHTSSTVAGVLVANASLYGIANGTARGAVPSARLAMYKVCWARSGCADMD 261

Query: 76  VLAGFEQAIADGVDLISVSIGSLPMDFFRDGQAIGAFHAMKKGILTSCAAGNDGPELSTV 135
           +LAGFE AI DGV++IS+SIG    D+  D  ++G+FHAM+KGILT  +AGNDGP   TV
Sbjct: 262 ILAGFEAAIHDGVEIISISIGGPIADYSSDSISVGSFHAMRKGILTVASAGNDGPSSGTV 321

Query: 136 ENVAPWIMTVAATAIDRRFVTAFKLG 160
            N  PWI+TVAA+ IDR F +   LG
Sbjct: 322 TNHEPWILTVAASGIDRTFKSKIDLG 347

[Close]
BLAST of Bhi03G001123 vs. TAIR10
Match: AT5G67360.1 (Subtilase family protein)

HSP 1 Score: 161.8 bits (408), Expect = 3.8e-40
Identity = 80/138 (57.97%), Postives = 99/138 (71.74%), Query Frame = 0 

Query: 22  GHGSHTASTVAGSTVEGASLYGLTKGTARGGVPSARIAVYKVCWNIFCSEMDVLAGFEQA 81
           GHG+HT+ST AGS VEGASL G   GTARG  P AR+AVYKVCW   C   D+LA  ++A
Sbjct: 211 GHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWLGGCFSSDILAAIDKA 270

Query: 82  IADGVDLISVSIGSLPMDFFRDGQAIGAFHAMKKGILTSCAAGNDGPELSTVENVAPWIM 141
           IAD V+++S+S+G    D++RDG AIGAF AM++GIL SC+AGN GP  S++ NVAPWI 
Sbjct: 271 IADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWIT 330

Query: 142 TVAATAIDRRFVTAFKLG 160
           TV A  +DR F     LG
Sbjct: 331 TVGAGTLDRDFPALAILG 348

[Close]
BLAST of Bhi03G001123 vs. TAIR10
Match: AT5G59190.1 (subtilase family protein)

HSP 1 Score: 160.6 bits (405), Expect = 8.4e-40
Identity = 79/139 (56.83%), Postives = 107/139 (76.98%), Query Frame = 0 

Query: 22  GHGSHTASTVAGSTVEGASLYGLTKGTARGGVPSARIAVYKVCWNIFCSEMDVLAGFEQA 81
           GHG+HTAST AG+ V+ AS YGL +GTARGGVPSARIA YKVC+N  C+++D+LA F+ A
Sbjct: 159 GHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCFN-RCNDVDILAAFDDA 218

Query: 82  IADGVDLISVSIGS-LPMDFFRDGQAIGAFHAMKKGILTSCAAGNDGPELSTVENVAPWI 141
           IADGVD+IS+SI +    +      AIG+FHAM +GI+T+ +AGN+GP+  +V NV+PW+
Sbjct: 219 IADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWM 278

Query: 142 MTVAATAIDRRFVTAFKLG 160
           +TVAA+  DR+F+    LG
Sbjct: 279 ITVAASGTDRQFIDRVVLG 296

[Close]
BLAST of Bhi03G001123 vs. TAIR10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 156.4 bits (394), Expect = 1.6e-38
Identity = 79/142 (55.63%), Postives = 94/142 (66.20%), Query Frame = 0 

Query: 22  GHGSHTASTVAGSTVEGASLYGLTKGTARGGVPSARIAVYKVCW----NIFCSEMDVLAG 81
           GHGSHT ST AG  V G S++G   GTA+GG P AR+A YKVCW       C + DVLA 
Sbjct: 222 GHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAA 281

Query: 82  FEQAIADGVDLISVSIGSLPMDFFRDGQAIGAFHAMKKGILTSCAAGNDGPELSTVENVA 141
           F+ AI DG D+ISVS+G  P  FF D  AIG+FHA KK I+  C+AGN GP  STV NVA
Sbjct: 282 FDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVA 341

Query: 142 PWIMTVAATAIDRRFVTAFKLG 160
           PW +TV A+ +DR F +   LG
Sbjct: 342 PWQITVGASTMDREFASNLVLG 363

[Close]
BLAST of Bhi03G001123 vs. TrEMBL
Match: tr|A0A1S4E3S0|A0A1S4E3S0_CUCME (subtilisin-like protease SBT4.15 OS=Cucumis melo OX=3656 GN=LOC103500883 PE=4 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 5.5e-62
Identity = 122/140 (87.14%), Postives = 131/140 (93.57%), Query Frame = 0 

Query: 20  SRGHGSHTASTVAGSTVEGASLYGLTKGTARGGVPSARIAVYKVCWNIFCSEMDVLAGFE 79
           + GHGSHTASTVAGS V GASLYGL KGTARGGVPSARIAVYKVCW+IFC+EMDVLA F+
Sbjct: 144 TEGHGSHTASTVAGSAVAGASLYGLAKGTARGGVPSARIAVYKVCWSIFCNEMDVLAAFD 203

Query: 80  QAIADGVDLISVSIGSLPMDFFRDGQAIGAFHAMKKGILTSCAAGNDGPELSTVENVAPW 139
           +AIADGVDLISVSIGS  MDFFRDGQAIGAFHAMKKGILT+ AAGNDGP+LSTVENVAPW
Sbjct: 204 EAIADGVDLISVSIGSPAMDFFRDGQAIGAFHAMKKGILTTAAAGNDGPQLSTVENVAPW 263

Query: 140 IMTVAATAIDRRFVTAFKLG 160
           IMTVAATAIDR FVT+F+LG
Sbjct: 264 IMTVAATAIDRGFVTSFELG 283

[Close]
BLAST of Bhi03G001123 vs. TrEMBL
Match: tr|A0A0A0KFB1|A0A0A0KFB1_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G451510 PE=4 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 1.6e-61
Identity = 121/138 (87.68%), Postives = 128/138 (92.75%), Query Frame = 0 

Query: 22  GHGSHTASTVAGSTVEGASLYGLTKGTARGGVPSARIAVYKVCWNIFCSEMDVLAGFEQA 81
           GHGSH ASTVAGS V GASLYGL KGTARGGVPSARIAVYKVCW++FC+EMDVLA F++A
Sbjct: 202 GHGSHIASTVAGSAVAGASLYGLAKGTARGGVPSARIAVYKVCWSVFCNEMDVLAAFDEA 261

Query: 82  IADGVDLISVSIGSLPMDFFRDGQAIGAFHAMKKGILTSCAAGNDGPELSTVENVAPWIM 141
           IADGVDLISVSIGS PMDFFRDGQAIGAFHAMKKGILT+ AAGNDGPEL TVENVAPWIM
Sbjct: 262 IADGVDLISVSIGSPPMDFFRDGQAIGAFHAMKKGILTTTAAGNDGPELFTVENVAPWIM 321

Query: 142 TVAATAIDRRFVTAFKLG 160
           TVAAT IDR FVTAF+LG
Sbjct: 322 TVAATGIDRGFVTAFELG 339

[Close]
BLAST of Bhi03G001123 vs. TrEMBL
Match: tr|A0A067LF77|A0A067LF77_JATCU (Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_23659 PE=4 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 4.0e-52
Identity = 104/139 (74.82%), Postives = 118/139 (84.89%), Query Frame = 0 

Query: 22  GHGSHTASTVAGSTVEGASLYGLTKGTARGGVPSARIAVYKVCW-NIFCSEMDVLAGFEQ 81
           GHG+HT+STVAG  V GASLYG+  GTARGGVPSARIA+YKVCW N  CS+MD+LAGF+ 
Sbjct: 169 GHGTHTSSTVAGVAVSGASLYGIANGTARGGVPSARIAMYKVCWENSGCSDMDLLAGFDD 228

Query: 82  AIADGVDLISVSIGSLPMDFFRDGQAIGAFHAMKKGILTSCAAGNDGPELSTVENVAPWI 141
           AIADGVD+IS SIG  P  FF D  AIG+FHAMKKGILTSC++GNDGP L+TVENVAPWI
Sbjct: 229 AIADGVDIISTSIGGWPRSFFEDPIAIGSFHAMKKGILTSCSSGNDGPTLATVENVAPWI 288

Query: 142 MTVAATAIDRRFVTAFKLG 160
           MTVAA+ IDRRF+T  KLG
Sbjct: 289 MTVAASTIDRRFITPIKLG 307

[Close]
BLAST of Bhi03G001123 vs. TrEMBL
Match: tr|A0A2I4G791|A0A2I4G791_9ROSI (subtilisin-like protease SBT4.15 OS=Juglans regia OX=51240 GN=LOC109005335 PE=4 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 5.7e-51
Identity = 104/147 (70.75%), Postives = 119/147 (80.95%), Query Frame = 0 

Query: 14  GQRKPISR-GHGSHTASTVAGSTVEGASLYGLTKGTARGGVPSARIAVYKVCWNIFCSEM 73
           G   P+   GHG+HT+ST AG  V GASLYG+ KGTARGGVPSARIA+YKVCW++ CS+M
Sbjct: 195 GNPSPVDNDGHGTHTSSTAAGVHVRGASLYGIAKGTARGGVPSARIAMYKVCWSVGCSDM 254

Query: 74  DVLAGFEQAIADGVDLISVSIGSLPMDFFRDGQAIGAFHAMKKGILTSCAAGNDGPELST 133
           D+LAGF++AIADGVDLISVSI   P D F D  AIGAFHAMKKGILTSC+AGNDGP   T
Sbjct: 255 DLLAGFDEAIADGVDLISVSIAGPPRDLFEDPIAIGAFHAMKKGILTSCSAGNDGPYSYT 314

Query: 134 VENVAPWIMTVAATAIDRRFVTAFKLG 160
           V+NVAPWIMTVAA+ IDR+F T   LG
Sbjct: 315 VQNVAPWIMTVAASGIDRQFKTEVNLG 341

[Close]
BLAST of Bhi03G001123 vs. TrEMBL
Match: tr|A0A2C9VF34|A0A2C9VF34_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_08G023600 PE=4 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 5.7e-51
Identity = 101/147 (68.71%), Postives = 123/147 (83.67%), Query Frame = 0 

Query: 14  GQRKPI-SRGHGSHTASTVAGSTVEGASLYGLTKGTARGGVPSARIAVYKVCWNIFCSEM 73
           GQ+ P    GHG+HT+S  AG  V GASLYG+  GTARGGVPSARIA+YKVCW   CS+M
Sbjct: 192 GQQSPADDDGHGTHTSSIAAGVAVNGASLYGIANGTARGGVPSARIAMYKVCWEGGCSDM 251

Query: 74  DVLAGFEQAIADGVDLISVSIGSLPMDFFRDGQAIGAFHAMKKGILTSCAAGNDGPELST 133
           D+LAGF+ AIADGVD++SVSIG    ++F D  +IG+FHAMKKGILTSC+AGN+GP+LST
Sbjct: 252 DLLAGFDDAIADGVDILSVSIGGFSRNYFEDPISIGSFHAMKKGILTSCSAGNNGPQLST 311

Query: 134 VENVAPWIMTVAATAIDRRFVTAFKLG 160
           VENVAPWIMTVAA++IDR+F+T+ KLG
Sbjct: 312 VENVAPWIMTVAASSIDRQFITSLKLG 338

[Close]
BLAST of Bhi03G001123 vs. NCBI nr
Match: XP_022962647.1 (subtilisin-like protease SBT4.15 [Cucurbita moschata])

HSP 1 Score: 253.8 bits (647), Expect = 4.0e-64
Identity = 125/140 (89.29%), Postives = 132/140 (94.29%), Query Frame = 0 

Query: 20  SRGHGSHTASTVAGSTVEGASLYGLTKGTARGGVPSARIAVYKVCWNIFCSEMDVLAGFE 79
           + GHGSHTASTVAGS VEGASLYGL KGTARGGVPSARIAVYKVCW+IFCS+MDVLAGF+
Sbjct: 153 TEGHGSHTASTVAGSAVEGASLYGLGKGTARGGVPSARIAVYKVCWSIFCSDMDVLAGFD 212

Query: 80  QAIADGVDLISVSIGSLPMDFFRDGQAIGAFHAMKKGILTSCAAGNDGPELSTVENVAPW 139
           +AIADGVD ISVSIGS+ +DFFRD  AIGAFHAMKKGILTSCAAGNDGPELSTVENVAPW
Sbjct: 213 EAIADGVDFISVSIGSVAVDFFRDSHAIGAFHAMKKGILTSCAAGNDGPELSTVENVAPW 272

Query: 140 IMTVAATAIDRRFVTAFKLG 160
           IMTVAAT IDRRFVTAFKLG
Sbjct: 273 IMTVAATGIDRRFVTAFKLG 292

[Close]
BLAST of Bhi03G001123 vs. NCBI nr
Match: XP_023518863.1 (subtilisin-like protease SBT4.15 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 252.3 bits (643), Expect = 1.2e-63
Identity = 124/140 (88.57%), Postives = 131/140 (93.57%), Query Frame = 0 

Query: 20  SRGHGSHTASTVAGSTVEGASLYGLTKGTARGGVPSARIAVYKVCWNIFCSEMDVLAGFE 79
           + GHGSHTASTVAGS VEGASLYGL KGTARGGVPSARIAVYKVCW+IFCS+MDVLAGF+
Sbjct: 107 TEGHGSHTASTVAGSAVEGASLYGLGKGTARGGVPSARIAVYKVCWSIFCSDMDVLAGFD 166

Query: 80  QAIADGVDLISVSIGSLPMDFFRDGQAIGAFHAMKKGILTSCAAGNDGPELSTVENVAPW 139
           +AIADGVD ISVSIGS+ +DFFRD  AIGAFHAMKKGILTSCAAGNDGPELSTVENVAPW
Sbjct: 167 EAIADGVDFISVSIGSVAVDFFRDSHAIGAFHAMKKGILTSCAAGNDGPELSTVENVAPW 226

Query: 140 IMTVAATAIDRRFVTAFKLG 160
           IMTVAAT IDRRF TAFKLG
Sbjct: 227 IMTVAATGIDRRFATAFKLG 246

[Close]
BLAST of Bhi03G001123 vs. NCBI nr
Match: XP_022972607.1 (subtilisin-like protease SBT4.15 [Cucurbita maxima])

HSP 1 Score: 251.1 bits (640), Expect = 2.6e-63
Identity = 124/140 (88.57%), Postives = 131/140 (93.57%), Query Frame = 0 

Query: 20  SRGHGSHTASTVAGSTVEGASLYGLTKGTARGGVPSARIAVYKVCWNIFCSEMDVLAGFE 79
           + GHGSHTASTVAGS VEGASLYGL KGTARGGVPSARIAVYKVCW+IFCS+MDVLAGF+
Sbjct: 150 TEGHGSHTASTVAGSAVEGASLYGLGKGTARGGVPSARIAVYKVCWSIFCSDMDVLAGFD 209

Query: 80  QAIADGVDLISVSIGSLPMDFFRDGQAIGAFHAMKKGILTSCAAGNDGPELSTVENVAPW 139
           +AIADGVD ISVSIGS+ +DFFRD  AIGAFHAMKKGILTSCAAGNDGPELSTV NVAPW
Sbjct: 210 EAIADGVDFISVSIGSVAVDFFRDSHAIGAFHAMKKGILTSCAAGNDGPELSTVVNVAPW 269

Query: 140 IMTVAATAIDRRFVTAFKLG 160
           IMTVAAT IDRRFVTAFKLG
Sbjct: 270 IMTVAATGIDRRFVTAFKLG 289

[Close]
BLAST of Bhi03G001123 vs. NCBI nr
Match: XP_022143781.1 (subtilisin-like protease SBT4.15 [Momordica charantia])

HSP 1 Score: 247.3 bits (630), Expect = 3.8e-62
Identity = 123/140 (87.86%), Postives = 130/140 (92.86%), Query Frame = 0 

Query: 20  SRGHGSHTASTVAGSTVEGASLYGLTKGTARGGVPSARIAVYKVCWNIFCSEMDVLAGFE 79
           + GHGSHTASTVAGS VEGASLYGL +GTARGGVPSARIAVYKVCW+IFCSEMDVLAGF+
Sbjct: 202 TEGHGSHTASTVAGSAVEGASLYGLARGTARGGVPSARIAVYKVCWSIFCSEMDVLAGFD 261

Query: 80  QAIADGVDLISVSIGSLPMDFFRDGQAIGAFHAMKKGILTSCAAGNDGPELSTVENVAPW 139
           QAIADGVDLISVSIGS PMD FRD QAIGAFHAMKKGILTS AAGN GP+LSTVENVAPW
Sbjct: 262 QAIADGVDLISVSIGSPPMDLFRDSQAIGAFHAMKKGILTSSAAGNYGPQLSTVENVAPW 321

Query: 140 IMTVAATAIDRRFVTAFKLG 160
           IMTVAATAIDRRF+T  +LG
Sbjct: 322 IMTVAATAIDRRFITVLQLG 341

[Close]
BLAST of Bhi03G001123 vs. NCBI nr
Match: XP_016902874.1 (PREDICTED: subtilisin-like protease SBT4.15, partial [Cucumis melo])

HSP 1 Score: 246.1 bits (627), Expect = 8.4e-62
Identity = 122/140 (87.14%), Postives = 131/140 (93.57%), Query Frame = 0 

Query: 20  SRGHGSHTASTVAGSTVEGASLYGLTKGTARGGVPSARIAVYKVCWNIFCSEMDVLAGFE 79
           + GHGSHTASTVAGS V GASLYGL KGTARGGVPSARIAVYKVCW+IFC+EMDVLA F+
Sbjct: 144 TEGHGSHTASTVAGSAVAGASLYGLAKGTARGGVPSARIAVYKVCWSIFCNEMDVLAAFD 203

Query: 80  QAIADGVDLISVSIGSLPMDFFRDGQAIGAFHAMKKGILTSCAAGNDGPELSTVENVAPW 139
           +AIADGVDLISVSIGS  MDFFRDGQAIGAFHAMKKGILT+ AAGNDGP+LSTVENVAPW
Sbjct: 204 EAIADGVDLISVSIGSPAMDFFRDGQAIGAFHAMKKGILTTAAAGNDGPQLSTVENVAPW 263

Query: 140 IMTVAATAIDRRFVTAFKLG 160
           IMTVAATAIDR FVT+F+LG
Sbjct: 264 IMTVAATAIDRGFVTSFELG 283

The following BLAST results are available for this feature:
BLAST of Bhi03G001123 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q9LZS6|SBT4F_ARATH2.5e-4965.22Subtilisin-like protease SBT4.15 OS=Arabidopsis thaliana OX=3702 GN=SBT4.15 PE=3... [more]
sp|Q9LLL8|SBT4E_ARATH8.6e-4259.59Subtilisin-like protease SBT4.14 OS=Arabidopsis thaliana OX=3702 GN=SBT4.14 PE=2... [more]
sp|Q39547|CUCM1_CUCME2.5e-4157.45Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1[more]
sp|O65351|SBT17_ARATH6.8e-3957.97Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... [more]
sp|Q9FIF8|SBT43_ARATH1.5e-3856.83Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 S... [more]
BLAST of Bhi03G001123 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G03620.11.4e-5065.22Subtilisin-like serine endopeptidase family protein[more]
AT4G00230.14.8e-4359.59xylem serine peptidase 1[more]
AT5G67360.13.8e-4057.97Subtilase family protein[more]
AT5G59190.18.4e-4056.83subtilase family protein[more]
AT2G04160.11.6e-3855.63Subtilisin-like serine endopeptidase family protein[more]
BLAST of Bhi03G001123 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A1S4E3S0|A0A1S4E3S0_CUCME5.5e-6287.14subtilisin-like protease SBT4.15 OS=Cucumis melo OX=3656 GN=LOC103500883 PE=4 SV... [more]
tr|A0A0A0KFB1|A0A0A0KFB1_CUCSA1.6e-6187.68Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G451510 PE=4 SV=1[more]
tr|A0A067LF77|A0A067LF77_JATCU4.0e-5274.82Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_23659 PE=4 SV=1[more]
tr|A0A2I4G791|A0A2I4G791_9ROSI5.7e-5170.75subtilisin-like protease SBT4.15 OS=Juglans regia OX=51240 GN=LOC109005335 PE=4 ... [more]
tr|A0A2C9VF34|A0A2C9VF34_MANES5.7e-5168.71Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_08G023600 PE=4 SV=... [more]
BLAST of Bhi03G001123 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022962647.14.0e-6489.29subtilisin-like protease SBT4.15 [Cucurbita moschata][more]
XP_023518863.11.2e-6388.57subtilisin-like protease SBT4.15 [Cucurbita pepo subsp. pepo][more]
XP_022972607.12.6e-6388.57subtilisin-like protease SBT4.15 [Cucurbita maxima][more]
XP_022143781.13.8e-6287.86subtilisin-like protease SBT4.15 [Momordica charantia][more]
XP_016902874.18.4e-6287.14PREDICTED: subtilisin-like protease SBT4.15, partial [Cucumis melo][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004252serine-type endopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: INTERPRO
TermDefinition
IPR000209Peptidase_S8/S53_dom
IPR036852Peptidase_S8/S53_dom_sf
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005575 cellular_component
molecular_function GO:0004252 serine-type endopeptidase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi03M001123Bhi03M001123mRNA


InterPro
Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036852Peptidase S8/S53 domain superfamilyGENE3DG3DSA:3.40.50.200coord: 20..148
e-value: 3.6E-60
score: 206.0
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILYSSF52743Subtilisin-likecoord: 21..157
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 15..135
e-value: 5.4E-15
score: 55.3
NoneNo IPR availableGENE3DG3DSA:3.50.30.30coord: 149..159
e-value: 3.6E-60
score: 206.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..30
NoneNo IPR availablePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 21..159
NoneNo IPR availablePANTHERPTHR10795:SF397SUBTILISIN-LIKE PROTEASE SBT4.15coord: 21..159

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Bhi03G001123Lsi01G012380Bottle gourd (USVL1VR-Ls)lsiwgoB170
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Bhi03G001123Wax gourdwgowgoB008
Bhi03G001123Cucumber (Gy14) v1cgywgoB102
Bhi03G001123Cucumber (Gy14) v2cgybwgoB320
Bhi03G001123Cucumber (Gy14) v2cgybwgoB599
Bhi03G001123Cucurbita maxima (Rimu)cmawgoB0355
Bhi03G001123Cucurbita maxima (Rimu)cmawgoB0791
Bhi03G001123Cucurbita maxima (Rimu)cmawgoB1037
Bhi03G001123Cucurbita maxima (Rimu)cmawgoB1061
Bhi03G001123Cucurbita moschata (Rifu)cmowgoB0345
Bhi03G001123Cucurbita moschata (Rifu)cmowgoB0783
Bhi03G001123Cucurbita moschata (Rifu)cmowgoB1031
Bhi03G001123Cucurbita moschata (Rifu)cmowgoB1054
Bhi03G001123Cucurbita pepo (Zucchini)cpewgoB0100
Bhi03G001123Cucurbita pepo (Zucchini)cpewgoB0610
Bhi03G001123Cucurbita pepo (Zucchini)cpewgoB0811
Bhi03G001123Cucurbita pepo (Zucchini)cpewgoB1131
Bhi03G001123Wild cucumber (PI 183967)cpiwgoB635
Bhi03G001123Cucumber (Chinese Long) v3cucwgoB336
Bhi03G001123Cucumber (Chinese Long) v3cucwgoB649
Bhi03G001123Cucumber (Chinese Long) v2cuwgoB629
Bhi03G001123Bottle gourd (USVL1VR-Ls)lsiwgoB332
Bhi03G001123Melon (DHL92) v3.6.1medwgoB423
Bhi03G001123Melon (DHL92) v3.6.1medwgoB645
Bhi03G001123Melon (DHL92) v3.5.1mewgoB421
Bhi03G001123Melon (DHL92) v3.5.1mewgoB639
Bhi03G001123Watermelon (Charleston Gray)wcgwgoB122
Bhi03G001123Watermelon (Charleston Gray)wcgwgoB391
Bhi03G001123Watermelon (97103) v2wgowmbB408
Bhi03G001123Watermelon (97103) v2wgowmbB432
Bhi03G001123Watermelon (97103) v1wgowmB409
Bhi03G001123Watermelon (97103) v1wgowmB450
Bhi03G001123Silver-seed gourdcarwgoB0306
Bhi03G001123Silver-seed gourdcarwgoB0329