Bhi03G001122 (gene) Wax gourd

NameBhi03G001122
Typegene
OrganismBenincasa hispida (Wax gourd)
DescriptionSubtilisin-like protease
Locationchr3 : 30849700 .. 30850286 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTGGCAGTGAAAAGATAGCGAGAGAGTCGAGAATCTACAGCTATGGAAAGAGCTTCAATGGATTCGCCGCAAGACTTTTGCCTCACGAAGCCACGAAGCTGTCAAGTAAATAATTAATTCAAATTGTATATAATAAATAAATAAACAAATTATTATTGTTCGACATGTCGTTTTAATTGTTGGACAGATGAGGAGGGTGTGGTTTCGGTGTTTGAGAGCAGAAAGCAGAGAGTTGTGACGACAAGATCCTGGGACTTTTTGGGATTAAATCATCGTTATTCTAAACGCAATCCTAAAATTGAGTCTAATCTCATTGTCGCTGTTTTGGATACGGGTGTCTCATCTCTTCTCTTTCATTAGAAATTTAAATTACTTCTTTATAAATTAATGGAATTATTAATCTTAATTTAGTTTTATTAATTGATGTTTAGGGATTTGGATTGACAGTCCTAGTTTCAGCGATGAAGGCTATGGTCCACCTCCCCCTAAATGGAAGGGCAAATGTGTCACTGGACCCAACTTCACAGCTTGCAACAAGTACATTTCTTCCTTCTCTCTCTATGTTAATGAAAATATCAGATGA

mRNA sequence

ATGATTGGCAGTGAAAAGATAGCGAGAGAGTCGAGAATCTACAGCTATGGAAAGAGCTTCAATGGATTCGCCGCAAGACTTTTGCCTCACGAAGCCACGAAGCTGTCAAATGAGGAGGGTGTGGTTTCGGTGTTTGAGAGCAGAAAGCAGAGAGTTGTGACGACAAGATCCTGGGACTTTTTGGGATTAAATCATCGTTATTCTAAACGCAATCCTAAAATTGAGTCTAATCTCATTGTCGCTGTTTTGGATACGGGGATTTGGATTGACAGTCCTAGTTTCAGCGATGAAGGCTATGGTCCACCTCCCCCTAAATGGAAGGGCAAATGTGTCACTGGACCCAACTTCACAGCTTGCAACAAGTACATTTCTTCCTTCTCTCTCTATGTTAATGAAAATATCAGATGA

Coding sequence (CDS)

ATGATTGGCAGTGAAAAGATAGCGAGAGAGTCGAGAATCTACAGCTATGGAAAGAGCTTCAATGGATTCGCCGCAAGACTTTTGCCTCACGAAGCCACGAAGCTGTCAAATGAGGAGGGTGTGGTTTCGGTGTTTGAGAGCAGAAAGCAGAGAGTTGTGACGACAAGATCCTGGGACTTTTTGGGATTAAATCATCGTTATTCTAAACGCAATCCTAAAATTGAGTCTAATCTCATTGTCGCTGTTTTGGATACGGGGATTTGGATTGACAGTCCTAGTTTCAGCGATGAAGGCTATGGTCCACCTCCCCCTAAATGGAAGGGCAAATGTGTCACTGGACCCAACTTCACAGCTTGCAACAAGTACATTTCTTCCTTCTCTCTCTATGTTAATGAAAATATCAGATGA

Protein sequence

MIGSEKIARESRIYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFESRKQRVVTTRSWDFLGLNHRYSKRNPKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNKYISSFSLYVNENIR
BLAST of Bhi03G001122 vs. Swiss-Prot
Match: sp|Q9LZS6|SBT4F_ARATH (Subtilisin-like protease SBT4.15 OS=Arabidopsis thaliana OX=3702 GN=SBT4.15 PE=3 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 3.1e-42
Identity = 81/123 (65.85%), Postives = 97/123 (78.86%), Query Frame = 0

Query: 1   MIGSEKIARESRIYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFESRKQRVVTTRSWDF 60
           +IG E  ARE +IYSYGK+ NGF ARL PHEA KLS EEGVVSVF++ ++++ TTRSWDF
Sbjct: 61  VIGDESKARELKIYSYGKNINGFVARLFPHEAEKLSREEGVVSVFKNTQRQLHTTRSWDF 120

Query: 61  LGLNHRYSKRNPKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACN 120
           LGL     KR+  IESN+IV VLDTGI ++SPSF+D+G GPPP KWKGKCVTG NFT CN
Sbjct: 121 LGLVESKYKRSVGIESNIIVGVLDTGIDVESPSFNDKGVGPPPAKWKGKCVTGNNFTRCN 180

Query: 121 KYI 124
             +
Sbjct: 181 NKV 183

BLAST of Bhi03G001122 vs. Swiss-Prot
Match: sp|Q39547|CUCM1_CUCME (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 1.9e-31
Identity = 67/134 (50.00%), Postives = 90/134 (67.16%), Query Frame = 0

Query: 1   MIGSEKIARESRIYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFESRKQRVVTTRSWDF 60
           ++GS   A ES +++Y +SFNGFA +L   EA K+++ EGVVSVF +    + TTRSWDF
Sbjct: 59  VVGS-TFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDF 118

Query: 61  LGLNHRYSKRNPKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACN 120
           LG      +R+ ++ESN++V VLDTGIW +SPSF DEG+ PPPPKWKG C T  NF    
Sbjct: 119 LGFPLTVPRRS-QVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNR 178

Query: 121 KYISSFSLYVNENI 135
           K I + S ++   I
Sbjct: 179 KIIGARSYHIGRPI 190

BLAST of Bhi03G001122 vs. Swiss-Prot
Match: sp|Q9FIG1|SBT4B_ARATH (Subtilisin-like protease SBT4.11 OS=Arabidopsis thaliana OX=3702 GN=SBT4.11 PE=2 SV=2)

HSP 1 Score: 134.4 bits (337), Expect = 9.3e-31
Identity = 65/127 (51.18%), Postives = 88/127 (69.29%), Query Frame = 0

Query: 5   EKIARESRI-----YSYGKSFNGFAARLLPHEATKLSNEEGVVSVFESRKQRVVTTRSWD 64
           +++ARES I      SY +SFNGF ARL   E  ++++ EGVVSVF ++K ++ T+ SWD
Sbjct: 60  QEVARESSIEGRLVRSYKRSFNGFVARLTESERERVADMEGVVSVFPNKKLKLQTSASWD 119

Query: 65  FLGLNH-RYSKRNPKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTA 124
           F+GL   + +KRNP +ES+ I+ V D GIW +S SFSD+G+GPPP KWKG C  G NFT 
Sbjct: 120 FMGLKEGKGTKRNPSVESDTIIGVFDGGIWPESESFSDKGFGPPPKKWKGICAGGKNFTC 179

Query: 125 CNKYISS 126
            NK I +
Sbjct: 180 NNKLIGA 186

BLAST of Bhi03G001122 vs. Swiss-Prot
Match: sp|Q9FIM5|SBT49_ARATH (Subtilisin-like protease SBT4.9 OS=Arabidopsis thaliana OX=3702 GN=SBT4.9 PE=2 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 1.7e-29
Identity = 65/124 (52.42%), Postives = 82/124 (66.13%), Query Frame = 0

Query: 13  IYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFESRKQRVVTTRSWDFLGLNH-RYSKRN 72
           + SY +SFNGFAARL   E  +++  EGVVSVF     ++ TT SWDFLGL   + +KRN
Sbjct: 73  VRSYKRSFNGFAARLTESERERVAEMEGVVSVFPDINYKLQTTASWDFLGLKEGKNTKRN 132

Query: 73  PKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNKYISSFSLYVN 132
             IES+ I+  +D+GIW +S SFSD+G+GPPP KWKG C  G NFT  NK I +   Y N
Sbjct: 133 LAIESDTIIGFIDSGIWPESESFSDKGFGPPPKKWKGVCSAGKNFTCNNKLIGARD-YTN 192

Query: 133 ENIR 136
           E  R
Sbjct: 193 EGTR 195

BLAST of Bhi03G001122 vs. Swiss-Prot
Match: sp|O23357|SBT42_ARATH (Subtilisin-like protease SBT4.2 OS=Arabidopsis thaliana OX=3702 GN=SBT4.2 PE=3 SV=3)

HSP 1 Score: 129.8 bits (325), Expect = 2.3e-29
Identity = 65/123 (52.85%), Postives = 84/123 (68.29%), Query Frame = 0

Query: 13  IYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFESRKQRVVTTRSWDFLGLNHRYSKRNP 72
           + SYG+SFNGFAA+L   E  KL   EGVVSVF S   ++ TTRS++F+GL  + S   P
Sbjct: 72  VRSYGRSFNGFAAKLTESEKDKLIGMEGVVSVFPSTVYKLFTTRSYEFMGLGDK-SNNVP 131

Query: 73  KIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNKYISSFSLYVNE 132
           ++ESN+IV V+D GIW +S SFSDEG GP P KWKG C  G NFT CN+ +     YV++
Sbjct: 132 EVESNVIVGVIDGGIWPESKSFSDEGIGPIPKKWKGTCAGGTNFT-CNRKVIGARHYVHD 191

Query: 133 NIR 136
           + R
Sbjct: 192 SAR 192

BLAST of Bhi03G001122 vs. TAIR10
Match: AT5G03620.1 (Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 172.6 bits (436), Expect = 1.7e-43
Identity = 81/123 (65.85%), Postives = 97/123 (78.86%), Query Frame = 0

Query: 1   MIGSEKIARESRIYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFESRKQRVVTTRSWDF 60
           +IG E  ARE +IYSYGK+ NGF ARL PHEA KLS EEGVVSVF++ ++++ TTRSWDF
Sbjct: 61  VIGDESKARELKIYSYGKNINGFVARLFPHEAEKLSREEGVVSVFKNTQRQLHTTRSWDF 120

Query: 61  LGLNHRYSKRNPKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACN 120
           LGL     KR+  IESN+IV VLDTGI ++SPSF+D+G GPPP KWKGKCVTG NFT CN
Sbjct: 121 LGLVESKYKRSVGIESNIIVGVLDTGIDVESPSFNDKGVGPPPAKWKGKCVTGNNFTRCN 180

Query: 121 KYI 124
             +
Sbjct: 181 NKV 183

BLAST of Bhi03G001122 vs. TAIR10
Match: AT5G58840.1 (Subtilase family protein)

HSP 1 Score: 130.2 bits (326), Expect = 9.7e-31
Identity = 65/124 (52.42%), Postives = 82/124 (66.13%), Query Frame = 0

Query: 13  IYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFESRKQRVVTTRSWDFLGLNH-RYSKRN 72
           + SY +SFNGFAARL   E  +++  EGVVSVF     ++ TT SWDFLGL   + +KRN
Sbjct: 73  VRSYKRSFNGFAARLTESERERVAEMEGVVSVFPDINYKLQTTASWDFLGLKEGKNTKRN 132

Query: 73  PKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNKYISSFSLYVN 132
             IES+ I+  +D+GIW +S SFSD+G+GPPP KWKG C  G NFT  NK I +   Y N
Sbjct: 133 LAIESDTIIGFIDSGIWPESESFSDKGFGPPPKKWKGVCSAGKNFTCNNKLIGARD-YTN 192

Query: 133 ENIR 136
           E  R
Sbjct: 193 EGTR 195

BLAST of Bhi03G001122 vs. TAIR10
Match: AT4G15040.1 (Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 129.8 bits (325), Expect = 1.3e-30
Identity = 65/123 (52.85%), Postives = 84/123 (68.29%), Query Frame = 0

Query: 13  IYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFESRKQRVVTTRSWDFLGLNHRYSKRNP 72
           + SYG+SFNGFAA+L   E  KL   EGVVSVF S   ++ TTRS++F+GL  + S   P
Sbjct: 34  VRSYGRSFNGFAAKLTESEKDKLIGMEGVVSVFPSTVYKLFTTRSYEFMGLGDK-SNNVP 93

Query: 73  KIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNKYISSFSLYVNE 132
           ++ESN+IV V+D GIW +S SFSDEG GP P KWKG C  G NFT CN+ +     YV++
Sbjct: 94  EVESNVIVGVIDGGIWPESKSFSDEGIGPIPKKWKGTCAGGTNFT-CNRKVIGARHYVHD 153

Query: 133 NIR 136
           + R
Sbjct: 154 SAR 154

BLAST of Bhi03G001122 vs. TAIR10
Match: AT5G59090.1 (subtilase 4.12)

HSP 1 Score: 129.8 bits (325), Expect = 1.3e-30
Identity = 65/124 (52.42%), Postives = 85/124 (68.55%), Query Frame = 0

Query: 13  IYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFESRKQRVVTTRSWDFLGLNH-RYSKRN 72
           + SY +SFNGFAARL   E T ++  EGVVSVF ++  ++ TT SWDF+G+   + +KRN
Sbjct: 70  VRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRN 129

Query: 73  PKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNKYISSFSLYVN 132
             IES+ I+ V+DTGIW +S SFSD+G+GPPP KWKG C  G NFT  NK I +   Y +
Sbjct: 130 LAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARD-YTS 189

Query: 133 ENIR 136
           E  R
Sbjct: 190 EGTR 192

BLAST of Bhi03G001122 vs. TAIR10
Match: AT5G59100.1 (Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 129.0 bits (323), Expect = 2.2e-30
Identity = 64/125 (51.20%), Postives = 82/125 (65.60%), Query Frame = 0

Query: 2   IGSEKIARESRIYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFESRKQRVVTTRSWDFL 61
           I  E +     + SY KSFNGFAARL   E  +L+  E VVSVF SRK ++ TT SW+F+
Sbjct: 61  ITGESLIENRLVRSYKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFM 120

Query: 62  GLNHRY-SKRNPKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACN 121
           GL     +KR   IES+ I+ V+D+GI+ +S SFSD+G+GPPP KWKG C  G NFT  N
Sbjct: 121 GLKEGIKTKRTRSIESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNN 180

Query: 122 KYISS 126
           K I +
Sbjct: 181 KVIGA 185

BLAST of Bhi03G001122 vs. TrEMBL
Match: tr|A0A1S4E3S0|A0A1S4E3S0_CUCME (subtilisin-like protease SBT4.15 OS=Cucumis melo OX=3656 GN=LOC103500883 PE=4 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 6.4e-61
Identity = 111/122 (90.98%), Postives = 117/122 (95.90%), Query Frame = 0

Query: 2   IGSEKIARESRIYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFESRKQRVVTTRSWDFL 61
           +GSEKIARES+IYSYGKSFNGFAARLLPHEATK+SNEE VVSVFESRK+RVVTTRSWDFL
Sbjct: 1   LGSEKIARESKIYSYGKSFNGFAARLLPHEATKISNEESVVSVFESRKKRVVTTRSWDFL 60

Query: 62  GLNHRYSKRNPKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNK 121
           GLNHRYSKRNP IESNLI+AV DTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACN 
Sbjct: 61  GLNHRYSKRNPLIESNLIIAVFDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNN 120

Query: 122 YI 124
            +
Sbjct: 121 KV 122

BLAST of Bhi03G001122 vs. TrEMBL
Match: tr|A0A0A0KFB1|A0A0A0KFB1_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G451510 PE=4 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 2.9e-53
Identity = 104/122 (85.25%), Postives = 111/122 (90.98%), Query Frame = 0

Query: 2   IGSEKIARESRIYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFESRKQRVVTTRSWDFL 61
           IG EKIARES+IYSYGKSFNGFAARLLP EATKLS+EE VVSVFESRK+RV+TTRSW+FL
Sbjct: 61  IGDEKIARESKIYSYGKSFNGFAARLLPDEATKLSDEESVVSVFESRKKRVLTTRSWEFL 120

Query: 62  GLNHRYSKRNPKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNK 121
           GLNH+YSKRNP IESNLIVAV DT    DSPSFSDEGYGPPPPKWKGKCVTGPNFTACN 
Sbjct: 121 GLNHQYSKRNPLIESNLIVAVFDT----DSPSFSDEGYGPPPPKWKGKCVTGPNFTACNN 178

Query: 122 YI 124
            +
Sbjct: 181 KV 178

BLAST of Bhi03G001122 vs. TrEMBL
Match: tr|V4SRZ5|V4SRZ5_9ROSI (Uncharacterized protein (Fragment) OS=Citrus clementina OX=85681 GN=CICLE_v10013548mg PE=4 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 2.6e-46
Identity = 85/132 (64.39%), Postives = 108/132 (81.82%), Query Frame = 0

Query: 2   IGSEKIARESRIYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFESRKQRVVTTRSWDFL 61
           IG EK+ARES+I+SYGKSFNGF ARLLPHEA +LS EE VVSVFE+ ++++ TTR+WDFL
Sbjct: 30  IGDEKLARESKIHSYGKSFNGFVARLLPHEAKRLSEEESVVSVFENTRRKLHTTRTWDFL 89

Query: 62  GLNHRYSKRNPKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNK 121
           G++ +  KRN K +SN+IV +LDTGIW++SPSF+D+G+GPPP KWKGKCVTG NFT CNK
Sbjct: 90  GMSEKLQKRNSKAQSNIIVGLLDTGIWVESPSFNDKGFGPPPAKWKGKCVTGANFTRCNK 149

Query: 122 YISSFSLYVNEN 134
            +     Y  +N
Sbjct: 150 KVIGARYYNLDN 161

BLAST of Bhi03G001122 vs. TrEMBL
Match: tr|A0A2H5MYV6|A0A2H5MYV6_CITUN (Uncharacterized protein OS=Citrus unshiu OX=55188 GN=CUMW_006160 PE=4 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 7.6e-46
Identity = 85/132 (64.39%), Postives = 107/132 (81.06%), Query Frame = 0

Query: 2   IGSEKIARESRIYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFESRKQRVVTTRSWDFL 61
           IG EK+ARES+I SYGKSFNGF ARLLPHEA +LS EE VVSVFE+ ++++ TTR+WDFL
Sbjct: 24  IGDEKLARESKIRSYGKSFNGFVARLLPHEAKRLSEEESVVSVFENTRRKLHTTRTWDFL 83

Query: 62  GLNHRYSKRNPKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNK 121
           G++ +  KRN K +SN+IV +LDTGIW++SPSF+D+G+GPPP KWKGKCVTG NFT CNK
Sbjct: 84  GMSEKLQKRNSKAQSNIIVGLLDTGIWVESPSFNDKGFGPPPAKWKGKCVTGANFTRCNK 143

Query: 122 YISSFSLYVNEN 134
            +     Y  +N
Sbjct: 144 KVIGARYYNLDN 155

BLAST of Bhi03G001122 vs. TrEMBL
Match: tr|A0A067F160|A0A067F160_CITSI (Uncharacterized protein OS=Citrus sinensis OX=2711 GN=CISIN_1g045363mg PE=4 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 2.9e-45
Identity = 84/132 (63.64%), Postives = 107/132 (81.06%), Query Frame = 0

Query: 2   IGSEKIARESRIYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFESRKQRVVTTRSWDFL 61
           IG EK+ARES+I SYGKSFNGF ARLLPHEA +LS EE VVSVFE+ ++++ TTR+WDFL
Sbjct: 24  IGDEKLARESKIRSYGKSFNGFVARLLPHEAKRLSEEESVVSVFENTRRKLHTTRTWDFL 83

Query: 62  GLNHRYSKRNPKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNK 121
           G++ +  KR+ K +SN+IV +LDTGIW++SPSF+D+G+GPPP KWKGKCVTG NFT CNK
Sbjct: 84  GMSEKLQKRSSKAQSNIIVGLLDTGIWVESPSFNDKGFGPPPAKWKGKCVTGANFTRCNK 143

Query: 122 YISSFSLYVNEN 134
            +     Y  +N
Sbjct: 144 KVIGARYYNLDN 155

BLAST of Bhi03G001122 vs. NCBI nr
Match: XP_016902874.1 (PREDICTED: subtilisin-like protease SBT4.15, partial [Cucumis melo])

HSP 1 Score: 242.3 bits (617), Expect = 9.7e-61
Identity = 111/122 (90.98%), Postives = 117/122 (95.90%), Query Frame = 0

Query: 2   IGSEKIARESRIYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFESRKQRVVTTRSWDFL 61
           +GSEKIARES+IYSYGKSFNGFAARLLPHEATK+SNEE VVSVFESRK+RVVTTRSWDFL
Sbjct: 1   LGSEKIARESKIYSYGKSFNGFAARLLPHEATKISNEESVVSVFESRKKRVVTTRSWDFL 60

Query: 62  GLNHRYSKRNPKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNK 121
           GLNHRYSKRNP IESNLI+AV DTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACN 
Sbjct: 61  GLNHRYSKRNPLIESNLIIAVFDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNN 120

Query: 122 YI 124
            +
Sbjct: 121 KV 122

BLAST of Bhi03G001122 vs. NCBI nr
Match: XP_004143378.1 (PREDICTED: subtilisin-like protease SBT4.14 [Cucumis sativus])

HSP 1 Score: 233.0 bits (593), Expect = 5.9e-58
Identity = 108/122 (88.52%), Postives = 115/122 (94.26%), Query Frame = 0

Query: 2   IGSEKIARESRIYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFESRKQRVVTTRSWDFL 61
           IG EKIARES+IYSYGKSFNGFAARLLP EATKLS+EE VVSVFESRK+RV+TTRSW+FL
Sbjct: 61  IGDEKIARESKIYSYGKSFNGFAARLLPDEATKLSDEESVVSVFESRKKRVLTTRSWEFL 120

Query: 62  GLNHRYSKRNPKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNK 121
           GLNH+YSKRNP IESNLIVAV DTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACN 
Sbjct: 121 GLNHQYSKRNPLIESNLIVAVFDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNN 180

Query: 122 YI 124
            +
Sbjct: 181 KV 182

BLAST of Bhi03G001122 vs. NCBI nr
Match: XP_022972607.1 (subtilisin-like protease SBT4.15 [Cucurbita maxima])

HSP 1 Score: 218.8 bits (556), Expect = 1.1e-53
Identity = 104/131 (79.39%), Postives = 115/131 (87.79%), Query Frame = 0

Query: 4   SEKIARESRIYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFESRKQRVVTTRSWDFLGL 63
           SE  ARES+I+SYG+SFNGFAARLLPHEA KLSN EGVVSVF SRK RVVTTRSWDFLGL
Sbjct: 10  SENKARESKIHSYGRSFNGFAARLLPHEAAKLSNVEGVVSVFPSRKHRVVTTRSWDFLGL 69

Query: 64  NHRYSKRNPKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNKYI 123
           NHR SKRNPKIESN+IVAVLDTGIWI+SPSFSDEGYGPPP KWKG+CVTGPNFTACN  +
Sbjct: 70  NHR-SKRNPKIESNIIVAVLDTGIWINSPSFSDEGYGPPPAKWKGRCVTGPNFTACNNKV 129

Query: 124 SSFSLYVNENI 135
              + +  +N+
Sbjct: 130 IGANYFDLDNV 139

BLAST of Bhi03G001122 vs. NCBI nr
Match: KGN48248.1 (hypothetical protein Csa_6G451510 [Cucumis sativus])

HSP 1 Score: 216.9 bits (551), Expect = 4.3e-53
Identity = 104/122 (85.25%), Postives = 111/122 (90.98%), Query Frame = 0

Query: 2   IGSEKIARESRIYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFESRKQRVVTTRSWDFL 61
           IG EKIARES+IYSYGKSFNGFAARLLP EATKLS+EE VVSVFESRK+RV+TTRSW+FL
Sbjct: 61  IGDEKIARESKIYSYGKSFNGFAARLLPDEATKLSDEESVVSVFESRKKRVLTTRSWEFL 120

Query: 62  GLNHRYSKRNPKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNK 121
           GLNH+YSKRNP IESNLIVAV DT    DSPSFSDEGYGPPPPKWKGKCVTGPNFTACN 
Sbjct: 121 GLNHQYSKRNPLIESNLIVAVFDT----DSPSFSDEGYGPPPPKWKGKCVTGPNFTACNN 178

Query: 122 YI 124
            +
Sbjct: 181 KV 178

BLAST of Bhi03G001122 vs. NCBI nr
Match: XP_022962647.1 (subtilisin-like protease SBT4.15 [Cucurbita moschata])

HSP 1 Score: 209.9 bits (533), Expect = 5.3e-51
Identity = 101/131 (77.10%), Postives = 113/131 (86.26%), Query Frame = 0

Query: 4   SEKIARESRIYSYGKSFNGFAARLLPHEATKLSNEEGVVSVFESRKQRVVTTRSWDFLGL 63
           SE  ARES+I+SYG+SFNGFAARLLPHEA KLSN EGVVSVF SRK RVVTTRSW+FLGL
Sbjct: 13  SENKARESKIHSYGRSFNGFAARLLPHEAAKLSNVEGVVSVFPSRKHRVVTTRSWNFLGL 72

Query: 64  NHRYSKRNPKIESNLIVAVLDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNKYI 123
           N R SKRN KIESN+IVAVLDTGIWI+SPSFSDEGYGPPP KWKG+CVTGPNFTACN  +
Sbjct: 73  NRR-SKRNLKIESNIIVAVLDTGIWINSPSFSDEGYGPPPAKWKGRCVTGPNFTACNNKV 132

Query: 124 SSFSLYVNENI 135
              + +  +N+
Sbjct: 133 IGANYFDLDNV 142

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
sp|Q9LZS6|SBT4F_ARATH3.1e-4265.85Subtilisin-like protease SBT4.15 OS=Arabidopsis thaliana OX=3702 GN=SBT4.15 PE=3... [more]
sp|Q39547|CUCM1_CUCME1.9e-3150.00Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1[more]
sp|Q9FIG1|SBT4B_ARATH9.3e-3151.18Subtilisin-like protease SBT4.11 OS=Arabidopsis thaliana OX=3702 GN=SBT4.11 PE=2... [more]
sp|Q9FIM5|SBT49_ARATH1.7e-2952.42Subtilisin-like protease SBT4.9 OS=Arabidopsis thaliana OX=3702 GN=SBT4.9 PE=2 S... [more]
sp|O23357|SBT42_ARATH2.3e-2952.85Subtilisin-like protease SBT4.2 OS=Arabidopsis thaliana OX=3702 GN=SBT4.2 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT5G03620.11.7e-4365.85Subtilisin-like serine endopeptidase family protein[more]
AT5G58840.19.7e-3152.42Subtilase family protein[more]
AT4G15040.11.3e-3052.85Subtilisin-like serine endopeptidase family protein[more]
AT5G59090.11.3e-3052.42subtilase 4.12[more]
AT5G59100.12.2e-3051.20Subtilisin-like serine endopeptidase family protein[more]
Match NameE-valueIdentityDescription
tr|A0A1S4E3S0|A0A1S4E3S0_CUCME6.4e-6190.98subtilisin-like protease SBT4.15 OS=Cucumis melo OX=3656 GN=LOC103500883 PE=4 SV... [more]
tr|A0A0A0KFB1|A0A0A0KFB1_CUCSA2.9e-5385.25Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G451510 PE=4 SV=1[more]
tr|V4SRZ5|V4SRZ5_9ROSI2.6e-4664.39Uncharacterized protein (Fragment) OS=Citrus clementina OX=85681 GN=CICLE_v10013... [more]
tr|A0A2H5MYV6|A0A2H5MYV6_CITUN7.6e-4664.39Uncharacterized protein OS=Citrus unshiu OX=55188 GN=CUMW_006160 PE=4 SV=1[more]
tr|A0A067F160|A0A067F160_CITSI2.9e-4563.64Uncharacterized protein OS=Citrus sinensis OX=2711 GN=CISIN_1g045363mg PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_016902874.19.7e-6190.98PREDICTED: subtilisin-like protease SBT4.15, partial [Cucumis melo][more]
XP_004143378.15.9e-5888.52PREDICTED: subtilisin-like protease SBT4.14 [Cucumis sativus][more]
XP_022972607.11.1e-5379.39subtilisin-like protease SBT4.15 [Cucurbita maxima][more]
KGN48248.14.3e-5385.25hypothetical protein Csa_6G451510 [Cucumis sativus][more]
XP_022962647.15.3e-5177.10subtilisin-like protease SBT4.15 [Cucurbita moschata][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004252serine-type endopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: INTERPRO
TermDefinition
IPR036852Peptidase_S8/S53_dom_sf
IPR010259S8pro/Inhibitor_I9
IPR037045S8pro/Inhibitor_I9_sf
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004252 serine-type endopeptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi03M001122Bhi03M001122mRNA


Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3DG3DSA:3.30.70.80coord: 1..52
e-value: 7.1E-15
score: 57.2
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 8..49
e-value: 1.6E-8
score: 35.0
IPR036852Peptidase S8/S53 domain superfamilyGENE3DG3DSA:3.40.50.200coord: 54..134
e-value: 4.7E-16
score: 60.7
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILYSSF52743Subtilisin-likecoord: 16..96
NoneNo IPR availablePANTHERPTHR10795:SF397SUBTILISIN-LIKE PROTEASE SBT4.15coord: 2..123
NoneNo IPR availablePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 2..123

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Bhi03G001122Cla97C10G202180Watermelon (97103) v2wgowmbB408
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Bhi03G001122Bhi11G001647Wax gourdwgowgoB008
The following block(s) are covering this gene:
GeneOrganismBlock
Bhi03G001122Cucumber (Gy14) v1cgywgoB102
Bhi03G001122Cucumber (Gy14) v2cgybwgoB320
Bhi03G001122Cucumber (Gy14) v2cgybwgoB599
Bhi03G001122Cucurbita maxima (Rimu)cmawgoB0355
Bhi03G001122Cucurbita maxima (Rimu)cmawgoB0791
Bhi03G001122Cucurbita maxima (Rimu)cmawgoB1037
Bhi03G001122Cucurbita maxima (Rimu)cmawgoB1061
Bhi03G001122Cucurbita moschata (Rifu)cmowgoB0345
Bhi03G001122Cucurbita moschata (Rifu)cmowgoB0783
Bhi03G001122Cucurbita moschata (Rifu)cmowgoB1031
Bhi03G001122Cucurbita moschata (Rifu)cmowgoB1054
Bhi03G001122Cucurbita pepo (Zucchini)cpewgoB0100
Bhi03G001122Cucurbita pepo (Zucchini)cpewgoB0610
Bhi03G001122Cucurbita pepo (Zucchini)cpewgoB0811
Bhi03G001122Cucurbita pepo (Zucchini)cpewgoB1131
Bhi03G001122Wild cucumber (PI 183967)cpiwgoB635
Bhi03G001122Cucumber (Chinese Long) v3cucwgoB336
Bhi03G001122Cucumber (Chinese Long) v3cucwgoB649
Bhi03G001122Cucumber (Chinese Long) v2cuwgoB629
Bhi03G001122Bottle gourd (USVL1VR-Ls)lsiwgoB170
Bhi03G001122Bottle gourd (USVL1VR-Ls)lsiwgoB332
Bhi03G001122Melon (DHL92) v3.6.1medwgoB423
Bhi03G001122Melon (DHL92) v3.6.1medwgoB645
Bhi03G001122Melon (DHL92) v3.5.1mewgoB421
Bhi03G001122Melon (DHL92) v3.5.1mewgoB639
Bhi03G001122Watermelon (Charleston Gray)wcgwgoB122
Bhi03G001122Watermelon (Charleston Gray)wcgwgoB391
Bhi03G001122Watermelon (97103) v2wgowmbB432
Bhi03G001122Watermelon (97103) v1wgowmB409
Bhi03G001122Watermelon (97103) v1wgowmB450
Bhi03G001122Silver-seed gourdcarwgoB0306
Bhi03G001122Silver-seed gourdcarwgoB0329