Bhi03G000521 (gene) Wax gourd

Overview
NameBhi03G000521
Typegene
OrganismBenincasa hispida (Wax gourd)
DescriptionCytochrome P450
Locationchr3 : 13042874 .. 13044499 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTGCTCTCAGCCATTACGGCTCTTACTGGCGCAAAATGCGAAAAATTGCCACGTTTGAGCTTCTATCCAATCGCAGAATGGAGCTGTTAAGGCAAGCCAAAGAGTCTGAAGTTAACCAAGCCGTAAAAGAGTTGTACCAAAGTTGGAAAGAAATTAGAAAAGACTCACAAGATCAGGACATCTTCACATTTAAATATTTTAATTATTTTTTGATATAAATGATTAGGGAGGATGAATAAAGATGAGTGCATGTTGAGATGCACCAATGTATTACCATAATAATAATTCAATTGCTTTAAAAAAATCCATCAGTTTTTTTAATTGGAGTACACAAAAGTTTTCTTTTTAATTTAATATTTAATATTATCTAATATTATTCTTTTATGAAAAGTTAAATGTATTTTTTTGTTATTTTACAAAGGAACTAGGAGTGTAACAAAGATAAAGGTCGAAAATTAAGCTCCCATTTGGTAATTATTTAGTTTTTTGTTTTTTTTTTTAGGTTGTCTATTTCATTCACTGCAATAATTTGCATCTTTCTTGAATATAATGGTTGAATTTTTAACCAAATTTCAAAAATAATTTTCAAAAACAAAAATAAAAAACAAAATGGTTACCAAACGGGAGTATAACTAATCCATTGGTTTAGTTTGGTTTTGTAGTATAAAATTGGACATCTCAATTGTATTTGGAAACAACTAAAAAATATTGATTCTAATCCATTTTTTATTAGAAAAGCCAATCAAAACCAAACAGAATCGAGAGTATCTACACTTAAAAATACACTTCTAGCATTAGGTATATCTTTGATATTTTAATTATTATGATGCATATATATTTGTTGTTTAGAAAAAAAAAATAAATTATTATAGAACATGTATGTTTGTTATTTAGAAAAGTGTCAACAAAAAATCAGAAAAAAAAAAGAAAAGAAAAAGAAACAGTCAAAAATCAAGAACCAAACTAATAATAAATCGAACTAATATAAAAACCAATTCGGAAAATTGATTTAGATCCATTCTTCATATAACCAGTTTAGTTTAATTTGGTTATTGGTTAACTTGAAAAGGGACAAAACTAAATCATCACCCCTGCTAAGAACATTCAAGTCATCTGGTTGAGTGTTGGAATATTGGGCCACGTAACTTGTGAACCCAATCCTTAAGTGGATAGGAATCTTGTATGAACAGGTGTTGGTGGAAATGAAGCAGTGGTTGGGGGAGTTGACATTGAACGTAATTCTGAGAGTGGTGGTTGGGAAAAGGAACTTTGGAGTTGGAGCAATGGTGGGAGAAGAAGATGAAGAAAAGGCCCGGCAGTGGCAGAAAGCGACGAGGGAGTTTTTACATTATTTGGGATTCTTTGTATTTGGTGATGTTGTTCAATATTTGGGATGGTTGGATATTGGCGGCCATGTGAAGGCCATGAAAAGGACCTCGAAGGTATTGGACCGAATGTTAACGCAGTGGTTGGAAGAGCACAAGAAAGACGGGGCTTTTGGTAATCCTGAAAAAGATAAAGACTTCATTGATGCAATGCTTTCAATTCTGAAGGATCCAACTCATGTTGATGTTCTTTCTCATGGCTTTCATCACGATACCATTATCAAATCAACATGTTTG

mRNA sequence

ATGTTTGCTCTCAGCCATTACGGCTCTTACTGGCGCAAAATGCGAAAAATTGCCACGTTTGAGCTTCTATCCAATCGCAGAATGGAGCTGTTAAGGCAAGCCAAAGAGTCTGAAGTTAACCAAGCCGTGTTGGTGGAAATGAAGCAGTGGTTGGGGGAGTTGACATTGAACGTAATTCTGAGAGTGGTGGTTGGGAAAAGGAACTTTGGAGTTGGAGCAATGGTGGGAGAAGAAGATGAAGAAAAGGCCCGGCAGTGGCAGAAAGCGACGAGGGAGTTTTTACATTATTTGGGATTCTTTGTATTTGGTGATGTTGTTCAATATTTGGGATGGTTGGATATTGGCGGCCATGTGAAGGCCATGAAAAGGACCTCGAAGGTATTGGACCGAATGTTAACGCAGTGGTTGGAAGAGCACAAGAAAGACGGGGCTTTTGGTAATCCTGAAAAAGATAAAGACTTCATTGATGCAATGCTTTCAATTCTGAAGGATCCAACTCATGTTGATGTTCTTTCTCATGGCTTTCATCACGATACCATTATCAAATCAACATGTTTG

Coding sequence (CDS)

ATGTTTGCTCTCAGCCATTACGGCTCTTACTGGCGCAAAATGCGAAAAATTGCCACGTTTGAGCTTCTATCCAATCGCAGAATGGAGCTGTTAAGGCAAGCCAAAGAGTCTGAAGTTAACCAAGCCGTGTTGGTGGAAATGAAGCAGTGGTTGGGGGAGTTGACATTGAACGTAATTCTGAGAGTGGTGGTTGGGAAAAGGAACTTTGGAGTTGGAGCAATGGTGGGAGAAGAAGATGAAGAAAAGGCCCGGCAGTGGCAGAAAGCGACGAGGGAGTTTTTACATTATTTGGGATTCTTTGTATTTGGTGATGTTGTTCAATATTTGGGATGGTTGGATATTGGCGGCCATGTGAAGGCCATGAAAAGGACCTCGAAGGTATTGGACCGAATGTTAACGCAGTGGTTGGAAGAGCACAAGAAAGACGGGGCTTTTGGTAATCCTGAAAAAGATAAAGACTTCATTGATGCAATGCTTTCAATTCTGAAGGATCCAACTCATGTTGATGTTCTTTCTCATGGCTTTCATCACGATACCATTATCAAATCAACATGTTTG

Protein sequence

MFALSHYGSYWRKMRKIATFELLSNRRMELLRQAKESEVNQAVLVEMKQWLGELTLNVILRVVVGKRNFGVGAMVGEEDEEKARQWQKATREFLHYLGFFVFGDVVQYLGWLDIGGHVKAMKRTSKVLDRMLTQWLEEHKKDGAFGNPEKDKDFIDAMLSILKDPTHVDVLSHGFHHDTIIKSTCL
Homology
[Close]
BLAST of Bhi03G000521 vs. Swiss-Prot
Match: sp|Q9SZ46|C82C4_ARATH (Cytochrome P450 82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=2 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 1.2e-36
Identity = 84/204 (41.18%), Postives = 121/204 (59.31%), Query Frame = 0 

Query: 1   MFALSHYGSYWRKMRKIATFELLSNRRMELLRQAKESEV----------------NQAVL 60
           +F  + Y ++WR+MRKIAT ELLSNRR+++L+  + SE+                 + V+
Sbjct: 117 VFGFAPYSAFWREMRKIATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKNGGTKPVM 176

Query: 61  VEMKQWLGELTLNVILRVVVGKRNFGVGAMVGEEDEEKARQWQKATREFLHYLGFFVFGD 120
           V++K WL ++TLN+I+R+V GKR FG G  V  ED E+A Q +KA  +F H +G F   D
Sbjct: 177 VDLKSWLEDMTLNMIVRMVAGKRYFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSD 236

Query: 121 VVQYLGWLDIGGHVKAMKRTSKVLDRMLTQWLEEHKKDGAF-GNPEKDKDFIDAMLSILK 180
               L + D+ GH K MK+T   LD +L +W+E H++   F G  E D DFID M+S+ +
Sbjct: 237 AFPTLSFFDLQGHEKEMKQTGSELDVILERWIENHRQQRKFSGTKENDSDFIDVMMSLAE 296

Query: 181 DPTHVDVLSH-GFHHDTIIKSTCL 187
                  LSH  +  +T IKSTCL
Sbjct: 297 Q----GKLSHLQYDANTSIKSTCL 316

[Close]
BLAST of Bhi03G000521 vs. Swiss-Prot
Match: sp|Q43068|C82A1_PEA (Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2)

HSP 1 Score: 153.3 bits (386), Expect = 2.7e-36
Identity = 89/219 (40.64%), Postives = 126/219 (57.53%), Query Frame = 0 

Query: 7   YGSYWRKMRKIATFELLSNRRMELLRQAKESEVNQA------------------------ 66
           YG+YWR++RKI T E+LSNRR+ELL   + SEV  +                        
Sbjct: 125 YGAYWRQLRKIVTLEILSNRRIELLSHIRVSEVQTSIKELVNVWSNQISSQYGLLDDTKS 184

Query: 67  ------------VLVEMKQWLGELTLNVILRVVVGKRNFGVGAMVGEEDEEKARQWQKAT 126
                       V VE+K+W  +LTLN++LR+VVGKR FG    V  E++E+A+++ +  
Sbjct: 185 SSTNDEPSTTDYVSVELKKWFAQLTLNMVLRMVVGKRCFG---DVDVENKEEAKRFLENI 244

Query: 127 REFLHYLGFFVFGDVVQYLGWLDIGGHVKAMKRTSKVLDRMLTQWLEEHKKDGAFGNPEK 186
           R+F+  +G F  GD V +L WLD+GGH K MK+ +K  D ML +WLEEH++    G+ +K
Sbjct: 245 RDFMRLIGTFTVGDGVPFLKWLDLGGHEKEMKKCAKKFDVMLNEWLEEHREKKGLGSEDK 304

[Close]
BLAST of Bhi03G000521 vs. Swiss-Prot
Match: sp|H2DH24|C7D47_PANGI (Cytochrome P450 CYP82D47 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 7.7e-36
Identity = 87/204 (42.65%), Postives = 127/204 (62.25%), Query Frame = 0 

Query: 1   MFALSHYGSYWRKMRKIATFELLSNRRMELLRQAKESEV-----------------NQAV 60
           MFA + Y S+W ++RKI + +LLS+RR+ELL+  + SE+                 +  V
Sbjct: 1   MFAFTPYSSFWVELRKITSLQLLSSRRLELLKHVRISEMEISMKQLYKIWSKKKDGSGRV 60

Query: 61  LVEMKQWLGELTLNVILRVVVGKRNFGVGAMVGEEDEEKARQWQKATREFLHYLGFFVFG 120
           LVEMK+  G+LTLNV+LR+V GKR FG G      D+E+AR+ ++  REF    G FV  
Sbjct: 61  LVEMKKLFGDLTLNVVLRMVAGKRYFGGG---NANDDEEARRCRRVWREFFRLHGVFVVA 120

Query: 121 DVVQYLGWLDIGGHVKAMKRTSKVLDRMLTQWLEEHK-KDGAFGNPEKDKDFIDAMLSIL 180
           D + +L WLD+GG+ KAMK+T+K +D +++ WLEEH+ K  +       +DF+D MLS +
Sbjct: 121 DSLPFLRWLDLGGYEKAMKKTAKEMDNIVSGWLEEHRMKRNSSDEDNTQQDFMDVMLSAV 180

Query: 181 KDPTHVDVLSHGFHHDTIIKSTCL 187
           K   +VD+   GF  D +IKSTC+
Sbjct: 181 K---NVDLC--GFDADVVIKSTCM 196

[Close]
BLAST of Bhi03G000521 vs. Swiss-Prot
Match: sp|O49858|C82A3_SOYBN (Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 7.7e-36
Identity = 86/202 (42.57%), Postives = 121/202 (59.90%), Query Frame = 0 

Query: 4   LSHYGSYWRKMRKIATFELLSNRRMELLRQAKESEVNQAV------------------LV 63
           L+ YG YWR++RKI TFE LSNRR+E     + SEV  ++                  LV
Sbjct: 125 LAPYGPYWRELRKIVTFEFLSNRRIEQRNHIRVSEVRTSIKELFDIWSNGNKNESRYTLV 184

Query: 64  EMKQWLGELTLNVILRVVVGKRNFGVGAMVGEEDEEKARQWQKATREFLHYLGFFVFGDV 123
           ++KQWL  LT N+++R+VVGKR FGV   +  E ++KA+++ K  REF++ +G F   D 
Sbjct: 185 DIKQWLAYLTFNMVVRMVVGKRYFGV---MHVEGKDKAQRFMKNIREFMNLMGTFTVADG 244

Query: 124 VQYLGWLDIGGHVKAMKRTSKVLDRMLTQWLEEHKKDGAFG-NPEKDKDFIDAMLSILKD 183
           V  L WLD+GGH KAMK  +K +D++L++WLEEH++    G N E D+DF+D M+S L  
Sbjct: 245 VPCLRWLDLGGHEKAMKANAKEVDKLLSEWLEEHRQKKLLGENVESDRDFMDVMISALN- 304

Query: 184 PTHVDVLSHGFHHDTIIKSTCL 187
                     F  DTI K+T L
Sbjct: 305 ----GAQIGAFDADTICKATSL 318

[Close]
BLAST of Bhi03G000521 vs. Swiss-Prot
Match: sp|O49394|C82C2_ARATH (Cytochrome P450 82C2 OS=Arabidopsis thaliana OX=3702 GN=CYP82C2 PE=2 SV=2)

HSP 1 Score: 149.1 bits (375), Expect = 5.0e-35
Identity = 82/205 (40.00%), Postives = 119/205 (58.05%), Query Frame = 0 

Query: 1   MFALSHYGSYWRKMRKIATFELLSNRRMELLRQAKESEV----------------NQAVL 60
           +F  + Y ++WR+MRKIAT ELLSNRR+++L+  + SE+                ++ V+
Sbjct: 117 VFGFAPYSAFWREMRKIATLELLSNRRLQMLKHVRVSEISMVMQDLYSLWVKKGGSEPVM 176

Query: 61  VEMKQWLGELTLNVILRVVVGKRNFGVGAMVGEEDEEKARQWQKATREFLHYLGFFVFGD 120
           V++K WL +++LN+++R+V GKR FG G  +  ED E+ARQ +K    F H +G F   D
Sbjct: 177 VDLKSWLEDMSLNMMVRMVAGKRYFG-GGSLSPEDAEEARQCRKGVANFFHLVGIFTVSD 236

Query: 121 VVQYLGWLDIGGHVKAMKRTSKVLDRMLTQWLEEHKKD-GAFGNPEKDKDFIDAMLSILK 180
               LGW D  GH K MK+T + LD +L +W+E H++     G    D DF+D MLS+ +
Sbjct: 237 AFPKLGWFDFQGHEKEMKQTGRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAE 296

Query: 181 DPTHVDVLSHGFHHDTI--IKSTCL 187
                   SH   HD I  IKSTCL
Sbjct: 297 Q----GKFSH-LQHDAITSIKSTCL 315

[Close]
BLAST of Bhi03G000521 vs. TAIR10
Match: AT4G31940.1 (cytochrome P450, family 82, subfamily C, polypeptide 4)

HSP 1 Score: 154.5 bits (389), Expect = 6.6e-38
Identity = 84/204 (41.18%), Postives = 121/204 (59.31%), Query Frame = 0 

Query: 1   MFALSHYGSYWRKMRKIATFELLSNRRMELLRQAKESEV----------------NQAVL 60
           +F  + Y ++WR+MRKIAT ELLSNRR+++L+  + SE+                 + V+
Sbjct: 117 VFGFAPYSAFWREMRKIATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKNGGTKPVM 176

Query: 61  VEMKQWLGELTLNVILRVVVGKRNFGVGAMVGEEDEEKARQWQKATREFLHYLGFFVFGD 120
           V++K WL ++TLN+I+R+V GKR FG G  V  ED E+A Q +KA  +F H +G F   D
Sbjct: 177 VDLKSWLEDMTLNMIVRMVAGKRYFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSD 236

Query: 121 VVQYLGWLDIGGHVKAMKRTSKVLDRMLTQWLEEHKKDGAF-GNPEKDKDFIDAMLSILK 180
               L + D+ GH K MK+T   LD +L +W+E H++   F G  E D DFID M+S+ +
Sbjct: 237 AFPTLSFFDLQGHEKEMKQTGSELDVILERWIENHRQQRKFSGTKENDSDFIDVMMSLAE 296

Query: 181 DPTHVDVLSH-GFHHDTIIKSTCL 187
                  LSH  +  +T IKSTCL
Sbjct: 297 Q----GKLSHLQYDANTSIKSTCL 316

[Close]
BLAST of Bhi03G000521 vs. TAIR10
Match: AT4G31970.1 (cytochrome P450, family 82, subfamily C, polypeptide 2)

HSP 1 Score: 149.1 bits (375), Expect = 2.8e-36
Identity = 82/205 (40.00%), Postives = 119/205 (58.05%), Query Frame = 0 

Query: 1   MFALSHYGSYWRKMRKIATFELLSNRRMELLRQAKESEV----------------NQAVL 60
           +F  + Y ++WR+MRKIAT ELLSNRR+++L+  + SE+                ++ V+
Sbjct: 117 VFGFAPYSAFWREMRKIATLELLSNRRLQMLKHVRVSEISMVMQDLYSLWVKKGGSEPVM 176

Query: 61  VEMKQWLGELTLNVILRVVVGKRNFGVGAMVGEEDEEKARQWQKATREFLHYLGFFVFGD 120
           V++K WL +++LN+++R+V GKR FG G  +  ED E+ARQ +K    F H +G F   D
Sbjct: 177 VDLKSWLEDMSLNMMVRMVAGKRYFG-GGSLSPEDAEEARQCRKGVANFFHLVGIFTVSD 236

Query: 121 VVQYLGWLDIGGHVKAMKRTSKVLDRMLTQWLEEHKKD-GAFGNPEKDKDFIDAMLSILK 180
               LGW D  GH K MK+T + LD +L +W+E H++     G    D DF+D MLS+ +
Sbjct: 237 AFPKLGWFDFQGHEKEMKQTGRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAE 296

Query: 181 DPTHVDVLSHGFHHDTI--IKSTCL 187
                   SH   HD I  IKSTCL
Sbjct: 297 Q----GKFSH-LQHDAITSIKSTCL 315

[Close]
BLAST of Bhi03G000521 vs. TAIR10
Match: AT4G31950.1 (cytochrome P450, family 82, subfamily C, polypeptide 3)

HSP 1 Score: 144.8 bits (364), Expect = 5.2e-35
Identity = 80/203 (39.41%), Postives = 115/203 (56.65%), Query Frame = 0 

Query: 3   ALSHYG-SYWRKMRKIATFELLSNRRMELLRQAKESEV----------------NQAVLV 62
           A  H G  +W +MRKIA  ELLSNRR+++L   + SE+                ++ V+V
Sbjct: 106 AAKHMGYVFWLEMRKIAMIELLSNRRLQMLNNVRVSEISMGVKDLYSLWVKKGGSEPVMV 165

Query: 63  EMKQWLGELTLNVILRVVVGKRNFGVGAMVGEEDEEKARQWQKATREFLHYLGFFVFGDV 122
           ++K WL ++  N+I+R+V GKR FG G     E  E+ARQW+K   +F H +G F   D 
Sbjct: 166 DLKSWLEDMIANMIMRMVAGKRYFGGGGAESSEHTEEARQWRKGIAKFFHLVGIFTVSDA 225

Query: 123 VQYLGWLDIGGHVKAMKRTSKVLDRMLTQWLEEHKKD-GAFGNPEKDKDFIDAMLSILKD 182
              LGWLD+ GH K MK+T + LD +L +W+E H++     G    D DF+D MLS+ + 
Sbjct: 226 FPKLGWLDLQGHEKEMKQTRRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAEQ 285

Query: 183 PTHVDVLSH-GFHHDTIIKSTCL 187
                 LSH  +  +T IK+TCL
Sbjct: 286 ----GKLSHLQYDANTCIKTTCL 304

[Close]
BLAST of Bhi03G000521 vs. TAIR10
Match: AT2G25160.1 (cytochrome P450, family 82, subfamily F, polypeptide 1)

HSP 1 Score: 94.7 bits (234), Expect = 6.2e-20
Identity = 62/175 (35.43%), Postives = 92/175 (52.57%), Query Frame = 0 

Query: 5   SHYGSYWRKMRKIATFELLSNRRMEL-----------------LRQAKESEVNQAVLVEM 64
           S YG YWR++RKIA  EL S   +++                 +R  K  +  + VLV+M
Sbjct: 121 SPYGLYWREIRKIAVSELFSTSGVDMHMVSRAREADLAFRALYVRWEKRGKPKEGVLVDM 180

Query: 65  KQWLGELTLNVILRVVVGKRNFGVGAMVGEEDEEKARQWQKATREFLHYLGFFVFGDVVQ 124
           KQ   +LT N+ L +V GKR FG        + ++AR+  K  REFL Y   F+  DV  
Sbjct: 181 KQEFIDLTANISLMMVSGKRYFGENPNC---EVKEARRCGKLIREFLDYFALFLLSDVAP 240

Query: 125 YLGWLDIGGHVKAMKRTSKVLDRMLTQWLEEHKKDGAFGNPEKDKDFIDAMLSIL 163
            LG+LD     + MKRT+K LD++   W+EEHK   +  +   + D++D ++ IL
Sbjct: 241 VLGFLD-WKTKRGMKRTAKGLDKVAEGWIEEHKNKRS-DHGRSENDYLDILIKIL 290

[Close]
BLAST of Bhi03G000521 vs. TAIR10
Match: AT1G01280.1 (cytochrome P450, family 703, subfamily A, polypeptide 2)

HSP 1 Score: 77.0 bits (188), Expect = 1.3e-14
Identity = 45/171 (26.32%), Postives = 87/171 (50.88%), Query Frame = 0 

Query: 3   ALSHYGSYWRKMRKIATFELLSNRRMELLRQAKESEVNQAVL-----------VEMKQWL 62
           AL+  G +W++MR+I    LL+ +R+E     +  E    +            + +K+ L
Sbjct: 116 ALAPMGPHWKRMRRICMEHLLTTKRLESFTTQRAEEARYLIRDVFKRSETGKPINLKEVL 175

Query: 63  GELTLNVILRVVVGKRNFGVGAMVGEEDEEKARQWQKATREFLHYLGFFVFGDVVQYLGW 122
           G  ++N + R+++GK+ FG G++V  ++   A+++   T +    LG    GD + +  W
Sbjct: 176 GAFSMNNVTRMLLGKQFFGPGSLVSPKE---AQEFLHITHKLFWLLGVIYLGDYLPFWRW 235

Query: 123 LDIGGHVKAMKRTSKVLDRMLTQWLEEHKKDGAFGNPEK-DKDFIDAMLSI 162
           +D  G  K M+   K +D   T+ ++EH++       +  D DF+D +LS+
Sbjct: 236 VDPSGCEKEMRDVEKRVDEFHTKIIDEHRRAKLEDEDKNGDMDFVDVLLSL 283

[Close]
BLAST of Bhi03G000521 vs. TrEMBL
Match: tr|M5XNX5|M5XNX5_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_ppa023495mg PE=3 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 1.5e-44
Identity = 104/203 (51.23%), Postives = 133/203 (65.52%), Query Frame = 0 

Query: 1   MFALSHYGSYWRKMRKIATFELLSNRRMELLRQAKESEVNQAV----------------- 60
           MF  S YGSYWR+MRK+ + ELLSNRR+ELL++ + SEV  ++                 
Sbjct: 124 MFGFSPYGSYWREMRKLTSLELLSNRRLELLKKVRVSEVEMSLKELYTLWIKRKESSGER 183

Query: 61  LVEMKQWLGELTLNVILRVVVGKRNFGVGAMVGEEDEEKARQWQKATREFLHYLGFFVFG 120
           LVEMKQW G+LTLNVI R+V GKR F  G +    +E++AR+WQKA REF H  G FV G
Sbjct: 184 LVEMKQWFGDLTLNVIFRMVAGKRCFMNGNL---SEEKEARRWQKAMREFFHLAGLFVLG 243

Query: 121 DVVQYLGWLDIGGHVKAMKRTSKVLDRMLTQWLEEHKKDGAFGNPEKDKDFIDAMLSILK 180
           D V +L WLD+GG  KAMK+T+K LD ++ +WLEEHK+    G   KD+DF+D MLS + 
Sbjct: 244 DAVPWLSWLDLGGQQKAMKKTAKELDSIVAEWLEEHKQKRTKG---KDQDFMDVMLSAI- 303

Query: 181 DPTHVDVLSHGFHHDTIIKSTCL 187
           D T V     GF  DT+IK+TCL
Sbjct: 304 DGTDV----AGFDADTVIKATCL 315

[Close]
BLAST of Bhi03G000521 vs. TrEMBL
Match: tr|A0A251R9S1|A0A251R9S1_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_1G387100 PE=3 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 1.5e-44
Identity = 104/203 (51.23%), Postives = 133/203 (65.52%), Query Frame = 0 

Query: 1   MFALSHYGSYWRKMRKIATFELLSNRRMELLRQAKESEVNQAV----------------- 60
           MF  S YGSYWR+MRK+ + ELLSNRR+ELL++ + SEV  ++                 
Sbjct: 135 MFGFSPYGSYWREMRKLTSLELLSNRRLELLKKVRVSEVEMSLKELYTLWIKRKESSGER 194

Query: 61  LVEMKQWLGELTLNVILRVVVGKRNFGVGAMVGEEDEEKARQWQKATREFLHYLGFFVFG 120
           LVEMKQW G+LTLNVI R+V GKR F  G +    +E++AR+WQKA REF H  G FV G
Sbjct: 195 LVEMKQWFGDLTLNVIFRMVAGKRCFMNGNL---SEEKEARRWQKAMREFFHLAGLFVLG 254

Query: 121 DVVQYLGWLDIGGHVKAMKRTSKVLDRMLTQWLEEHKKDGAFGNPEKDKDFIDAMLSILK 180
           D V +L WLD+GG  KAMK+T+K LD ++ +WLEEHK+    G   KD+DF+D MLS + 
Sbjct: 255 DAVPWLSWLDLGGQQKAMKKTAKELDSIVAEWLEEHKQKRTKG---KDQDFMDVMLSAI- 314

Query: 181 DPTHVDVLSHGFHHDTIIKSTCL 187
           D T V     GF  DT+IK+TCL
Sbjct: 315 DGTDV----AGFDADTVIKATCL 326

[Close]
BLAST of Bhi03G000521 vs. TrEMBL
Match: tr|A0A2I4EQH4|A0A2I4EQH4_9ROSI (cytochrome P450 CYP82D47-like OS=Juglans regia OX=51240 GN=LOC108991732 PE=3 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 5.7e-44
Identity = 100/207 (48.31%), Postives = 131/207 (63.29%), Query Frame = 0 

Query: 1   MFALSHYGSYWRKMRKIATFELLSNRRMELLRQAKESEVNQA------------------ 60
           MF  S YGSYWR++RKI + ELLSNRR+ELL+  + SE+  +                  
Sbjct: 123 MFGFSPYGSYWREIRKIISLELLSNRRLELLKDVRASEMETSLKELYKVWTEKKDVSDGG 182

Query: 61  ---VLVEMKQWLGELTLNVILRVVVGKRNFGVGAMVGEEDEEKARQWQKATREFLHYLGF 120
              V VEMKQW G+LTLNV+LR+V GKR FG  A   +E    A++ QKA REF H +G 
Sbjct: 183 GGGVSVEMKQWFGDLTLNVVLRMVAGKRYFGAAAAADKE----AQRCQKAFREFFHLMGQ 242

Query: 121 FVFGDVVQYLGWLDIGGHVKAMKRTSKVLDRMLTQWLEEHKKDGAFGNPEKDKDFIDAML 180
           F+ GD + YLGW+D+GGHVKAMK+T+K +D + T+WLEEH+K+   G  ++  DFID ML
Sbjct: 243 FLVGDAIPYLGWMDLGGHVKAMKKTAKEMDCLATEWLEEHRKNRVSGESKEQLDFIDIML 302

Query: 181 SILKDPTHVDVLSHGFHHDTIIKSTCL 187
           S+L+     D     F  DT+ KSTCL
Sbjct: 303 SVLEGADLGD-----FDADTVNKSTCL 320

[Close]
BLAST of Bhi03G000521 vs. TrEMBL
Match: tr|M5WY14|M5WY14_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_3G064700 PE=3 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 9.8e-44
Identity = 102/203 (50.25%), Postives = 132/203 (65.02%), Query Frame = 0 

Query: 1   MFALSHYGSYWRKMRKIATFELLSNRRMELLRQAKESEVNQA-----------------V 60
           MF  S YG+YWR++RK+ + ELLSNRR+ELLR  + SEV  +                 +
Sbjct: 124 MFGFSPYGTYWREIRKLTSLELLSNRRLELLRNVRASEVEMSLKELYTLWSNRKEGPGEL 183

Query: 61  LVEMKQWLGELTLNVILRVVVGKRNFGVGAMVGEEDEEKARQWQKATREFLHYLGFFVFG 120
           LV+MKQW G LTLNVI R++ GKR F  G +    +E++AR+WQKA  EF H+LG F+ G
Sbjct: 184 LVDMKQWFGGLTLNVIFRMIAGKRCFMNGDL---SEEKEARRWQKAMGEFFHFLGLFLLG 243

Query: 121 DVVQYLGWLDIGGHVKAMKRTSKVLDRMLTQWLEEHKKDGAFGNPEKDKDFIDAMLSILK 180
           D V +L WLD+GG  KAMKRT+K LD +L +WLEEHK+    G   KD+DFID MLS + 
Sbjct: 244 DAVPWLSWLDLGGQQKAMKRTAKELDSILAEWLEEHKQKRTKG---KDQDFIDVMLSAI- 303

Query: 181 DPTHVDVLSHGFHHDTIIKSTCL 187
           D  +V     GF  DT+IK+TCL
Sbjct: 304 DGANV----AGFDADTVIKATCL 315

[Close]
BLAST of Bhi03G000521 vs. TrEMBL
Match: tr|F6H019|F6H019_VITVI (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_18s0001g11520 PE=3 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 1.3e-43
Identity = 104/205 (50.73%), Postives = 133/205 (64.88%), Query Frame = 0 

Query: 1   MFALSHYGSYWRKMRKIATFELLSNRRMELLRQAKESEVNQ-----------------AV 60
           MFA S +G+YWR++RKIAT ELLSNRR+ELL+  + SEV                    V
Sbjct: 123 MFAFSPHGAYWREVRKIATLELLSNRRLELLKNVRISEVETCMKELYKLWAEKKSEAGVV 182

Query: 61  LVEMKQWLGELTLNVILRVVVGKRNFGVGAMVGEEDEEKARQWQKATREFLHYLGFFVFG 120
           LV+MKQW G L+LNVIL++VVGKR FG  A   E  E++A+Q QKA REF   LG FV  
Sbjct: 183 LVDMKQWFGHLSLNVILKMVVGKRYFGYAA---ESKEKEAQQCQKAIREFFRLLGLFVVS 242

Query: 121 DVVQYLGWLDIGGHVKAMKRTSKVLDRMLTQWLEEHKKDGAFGNPEKDKDFIDAMLSIL- 180
           D + +LGWLD+GGHVKAMK+T+K LD +  +WLEEH++    G  + D+DF+D MLSIL 
Sbjct: 243 DALPFLGWLDVGGHVKAMKKTAKELDGIAQEWLEEHRRRKDSGEADGDQDFMDVMLSILG 302

Query: 181 -KDPTHVDVLSHGFHHDTIIKSTCL 187
             DP       +G+  DTI K+T L
Sbjct: 303 ATDP-------NGYDADTINKATSL 317

[Close]
BLAST of Bhi03G000521 vs. NCBI nr
Match: XP_022132489.1 (cytochrome P450 CYP82D47-like [Momordica charantia] >XP_022132490.1 cytochrome P450 CYP82D47-like [Momordica charantia])

HSP 1 Score: 225.3 bits (573), Expect = 1.7e-55
Identity = 120/205 (58.54%), Postives = 141/205 (68.78%), Query Frame = 0 

Query: 1   MFALSHYGSYWRKMRKIATFELLSNRRMELLRQAKESEVNQA-----------------V 60
           MFA   Y SYWRKMRK     LLSNRR+ELLR  +ESEVNQA                 V
Sbjct: 125 MFAFGPYDSYWRKMRKTTNLHLLSNRRLELLRNVRESEVNQAIKELYTAWKERRDEQGQV 184

Query: 61  LVEMKQWLGELTLNVILRVVVGKRNFGVGAMVGEEDEEKARQWQKATREFLHYLGFFVFG 120
           LVEMKQW+G+LT+N+IL VV GKR FG  AMV E +   AR   +AT+EFL  +G F  G
Sbjct: 185 LVEMKQWVGDLTMNLILGVVAGKRYFGAAAMVAEAE---ARACHRATKEFLRLMGLFTLG 244

Query: 121 DVVQYLGWLDIGGHVKAMKRTSKVLDRMLTQWLEEHKKDGAFGNPEKDKDFIDAMLSILK 180
           DVV YLGWLD+GGHVKAMKRTSK LD MLT+WLEEH+KD A G  ++DKDF+D MLS+L+
Sbjct: 245 DVVPYLGWLDVGGHVKAMKRTSKELDHMLTRWLEEHRKDRASGRSKEDKDFMDVMLSVLE 304

Query: 181 DPTH--VDVLSHGFHHDTIIKSTCL 187
           + T   V   +H F  DTIIK+TCL
Sbjct: 305 EETANVVSDSAHSFDDDTIIKATCL 326

[Close]
BLAST of Bhi03G000521 vs. NCBI nr
Match: XP_022133138.1 (cytochrome P450 CYP82D47-like [Momordica charantia])

HSP 1 Score: 216.9 bits (551), Expect = 6.0e-53
Identity = 118/206 (57.28%), Postives = 140/206 (67.96%), Query Frame = 0 

Query: 1   MFALSHYGSYWRKMRKIATFELLSNRRMELLRQAKESEVNQAV----------------- 60
           +FA   Y SYWRKMRK  T  LLS+RR+ELLR  +ESEVNQA+                 
Sbjct: 128 VFAFGPYDSYWRKMRKTTTLHLLSSRRLELLRDVRESEVNQAIKELYQIWKERRDKQGQG 187

Query: 61  LVEMKQWLGELTLNVILRVVVGKRNFGVGAMVGEEDEEKARQWQKATREFLHYLGFFVFG 120
           LVEMKQW+G+LT+N+IL VV GKR FG  AMV   +E +AR  QKA +EFL  +G F+ G
Sbjct: 188 LVEMKQWVGDLTMNLILGVVAGKRYFGPTAMV---EEAEARACQKAMKEFLRLMGLFILG 247

Query: 121 DVVQYLGWLDIGGHVKAMKRTSKVLDRMLTQWLEEHKKDGAFGNPEKDKDFIDAMLSILK 180
           D V YLGWLD+GGHVKAMKRTSK LD +LTQWLEEHKK  A G  ++DKDF+D MLS L+
Sbjct: 248 DAVPYLGWLDVGGHVKAMKRTSKELDHILTQWLEEHKKKRASGKSKEDKDFMDVMLSTLE 307

Query: 181 DPTHVDVLS---HGFHHDTIIKSTCL 187
           +     V S   H F  DTIIK+TCL
Sbjct: 308 ETPDNVVASDSIHSFDVDTIIKATCL 330

[Close]
BLAST of Bhi03G000521 vs. NCBI nr
Match: XP_022133139.1 (cytochrome P450 CYP82D47-like [Momordica charantia])

HSP 1 Score: 216.1 bits (549), Expect = 1.0e-52
Identity = 116/205 (56.59%), Postives = 137/205 (66.83%), Query Frame = 0 

Query: 1   MFALSHYGSYWRKMRKIATFELLSNRRMELLRQAKESEVNQAV----------------- 60
           MF    Y SYWRKMRK     LLSNRR+ELLR  +ESEV+QA+                 
Sbjct: 1   MFGFGPYNSYWRKMRKTTNVHLLSNRRLELLRNVRESEVSQAIKELYQIWKKRRDGQGRG 60

Query: 61  LVEMKQWLGELTLNVILRVVVGKRNFGVGAMVGEEDEEKARQWQKATREFLHYLGFFVFG 120
           LVEMKQW+GELT+N+IL VV  KR FG  AMV   +E++AR  QKAT+EFL  +G F  G
Sbjct: 61  LVEMKQWIGELTMNLILGVVASKRYFGPAAMV---EEDEARACQKATKEFLRLMGLFTLG 120

Query: 121 DVVQYLGWLDIGGHVKAMKRTSKVLDRMLTQWLEEHKKDGAFGNPEKDKDFIDAMLSILK 180
           D   +LGWLD+GGHVKAMKRTSK LD +LTQWLEEHKKD A G  ++DKDF+D MLS L+
Sbjct: 121 DAAPHLGWLDVGGHVKAMKRTSKELDHILTQWLEEHKKDRASGGSKEDKDFMDVMLSTLE 180

Query: 181 DPTHVDVLS--HGFHHDTIIKSTCL 187
           +     V    H F  DTIIK+TCL
Sbjct: 181 ETPDAVVSDSIHSFDVDTIIKATCL 202

[Close]
BLAST of Bhi03G000521 vs. NCBI nr
Match: XP_021675767.1 (cytochrome P450 CYP82D47-like [Hevea brasiliensis])

HSP 1 Score: 189.9 bits (481), Expect = 7.8e-45
Identity = 100/203 (49.26%), Postives = 135/203 (66.50%), Query Frame = 0 

Query: 1   MFALSHYGSYWRKMRKIATFELLSNRRMELLRQAKESEVNQA-----------------V 60
           MF L+ Y +YWR++RKIAT ELLSNRR+ELL+  +  EV  +                 V
Sbjct: 59  MFGLAPYTAYWREIRKIATSELLSNRRLELLKHVRIYEVETSLKELYKLWTQRTNGLGQV 118

Query: 61  LVEMKQWLGELTLNVILRVVVGKRNFGVGAMVGEEDEEKARQWQKATREFLHYLGFFVFG 120
            VE+KQWLG+L LNVIL+++ GK  FG  A+   +DE+ AR+WQKA REF HYLG FV  
Sbjct: 119 KVELKQWLGDLNLNVILKMIAGKTYFGASAV---DDEKVARRWQKAMREFFHYLGIFVVR 178

Query: 121 DVVQYLGWLDIGGHVKAMKRTSKVLDRMLTQWLEEHKKDGAFGNPEKDKDFIDAMLSILK 180
           D V +LGWLD+GGH KAM+RT+  LD ++ +WLEEHK++ A G   +++DF+D MLS+L+
Sbjct: 179 DAVPFLGWLDLGGHEKAMRRTALELDSIIGEWLEEHKRNRASGEAREEQDFMDVMLSVLE 238

Query: 181 DPTHVDVLSHGFHHDTIIKSTCL 187
           D +       G+  DTI K+T L
Sbjct: 239 DKSIA-----GYDADTINKATSL 253

[Close]
BLAST of Bhi03G000521 vs. NCBI nr
Match: XP_007226598.2 (cytochrome P450 CYP82D47 [Prunus persica] >ONI32809.1 hypothetical protein PRUPE_1G387100 [Prunus persica])

HSP 1 Score: 188.3 bits (477), Expect = 2.3e-44
Identity = 104/203 (51.23%), Postives = 133/203 (65.52%), Query Frame = 0 

Query: 1   MFALSHYGSYWRKMRKIATFELLSNRRMELLRQAKESEVNQAV----------------- 60
           MF  S YGSYWR+MRK+ + ELLSNRR+ELL++ + SEV  ++                 
Sbjct: 135 MFGFSPYGSYWREMRKLTSLELLSNRRLELLKKVRVSEVEMSLKELYTLWIKRKESSGER 194

Query: 61  LVEMKQWLGELTLNVILRVVVGKRNFGVGAMVGEEDEEKARQWQKATREFLHYLGFFVFG 120
           LVEMKQW G+LTLNVI R+V GKR F  G +    +E++AR+WQKA REF H  G FV G
Sbjct: 195 LVEMKQWFGDLTLNVIFRMVAGKRCFMNGNL---SEEKEARRWQKAMREFFHLAGLFVLG 254

Query: 121 DVVQYLGWLDIGGHVKAMKRTSKVLDRMLTQWLEEHKKDGAFGNPEKDKDFIDAMLSILK 180
           D V +L WLD+GG  KAMK+T+K LD ++ +WLEEHK+    G   KD+DF+D MLS + 
Sbjct: 255 DAVPWLSWLDLGGQQKAMKKTAKELDSIVAEWLEEHKQKRTKG---KDQDFMDVMLSAI- 314

Query: 181 DPTHVDVLSHGFHHDTIIKSTCL 187
           D T V     GF  DT+IK+TCL
Sbjct: 315 DGTDV----AGFDADTVIKATCL 326

The following BLAST results are available for this feature:
BLAST of Bhi03G000521 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q9SZ46|C82C4_ARATH1.2e-3641.18Cytochrome P450 82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=2 SV=1[more]
sp|Q43068|C82A1_PEA2.7e-3640.64Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2[more]
sp|H2DH24|C7D47_PANGI7.7e-3642.65Cytochrome P450 CYP82D47 OS=Panax ginseng OX=4054 PE=2 SV=1[more]
sp|O49858|C82A3_SOYBN7.7e-3642.57Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1[more]
sp|O49394|C82C2_ARATH5.0e-3540.00Cytochrome P450 82C2 OS=Arabidopsis thaliana OX=3702 GN=CYP82C2 PE=2 SV=2[more]
BLAST of Bhi03G000521 vs. TAIR10
Match NameE-valueIdentityDescription
AT4G31940.16.6e-3841.18cytochrome P450, family 82, subfamily C, polypeptide 4[more]
AT4G31970.12.8e-3640.00cytochrome P450, family 82, subfamily C, polypeptide 2[more]
AT4G31950.15.2e-3539.41cytochrome P450, family 82, subfamily C, polypeptide 3[more]
AT2G25160.16.2e-2035.43cytochrome P450, family 82, subfamily F, polypeptide 1[more]
AT1G01280.11.3e-1426.32cytochrome P450, family 703, subfamily A, polypeptide 2[more]
BLAST of Bhi03G000521 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|M5XNX5|M5XNX5_PRUPE1.5e-4451.23Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_ppa023495mg PE=3 SV=1[more]
tr|A0A251R9S1|A0A251R9S1_PRUPE1.5e-4451.23Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_1G387100 PE=3 SV=1[more]
tr|A0A2I4EQH4|A0A2I4EQH4_9ROSI5.7e-4448.31cytochrome P450 CYP82D47-like OS=Juglans regia OX=51240 GN=LOC108991732 PE=3 SV=... [more]
tr|M5WY14|M5WY14_PRUPE9.8e-4450.25Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_3G064700 PE=3 SV=1[more]
tr|F6H019|F6H019_VITVI1.3e-4350.73Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_18s0001g11520 PE=3 SV=... [more]
BLAST of Bhi03G000521 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022132489.11.7e-5558.54cytochrome P450 CYP82D47-like [Momordica charantia] >XP_022132490.1 cytochrome P... [more]
XP_022133138.16.0e-5357.28cytochrome P450 CYP82D47-like [Momordica charantia][more]
XP_022133139.11.0e-5256.59cytochrome P450 CYP82D47-like [Momordica charantia][more]
XP_021675767.17.8e-4549.26cytochrome P450 CYP82D47-like [Hevea brasiliensis][more]
XP_007226598.22.3e-4451.23cytochrome P450 CYP82D47 [Prunus persica] >ONI32809.1 hypothetical protein PRUPE... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0016705oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0005506iron ion binding
GO:0020037heme binding
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: INTERPRO
TermDefinition
IPR036396Cyt_P450_sf
IPR001128Cyt_P450
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi03M000521Bhi03M000521mRNA


InterPro
Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PFAMPF00067p450coord: 8..161
e-value: 1.9E-7
score: 30.1
IPR036396Cytochrome P450 superfamilyGENE3DG3DSA:1.10.630.10coord: 1..186
e-value: 3.8E-21
score: 77.2
IPR036396Cytochrome P450 superfamilySUPERFAMILYSSF48264Cytochrome P450coord: 4..169
NoneNo IPR availablePANTHERPTHR24298:SF340SUBFAMILY NOT NAMEDcoord: 1..185
NoneNo IPR availablePANTHERPTHR24298FAMILY NOT NAMEDcoord: 1..185