Bhi03G000482 (gene) Wax gourd

NameBhi03G000482
Typegene
OrganismBenincasa hispida (Wax gourd)
Description1-aminocyclopropane-1-carboxylate oxidase
Locationchr3 : 12200043 .. 12201790 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACCTTTCCCACTATCTTTTTCCTTCCTTAATTTTCCACTTTCTTTTATTTCCCCTTCCCCTATAAATACCAGCCCATCTCCACCACTCAAAACACACACTCAAAACACTAAGAAAGTTACAAAGTTTTTGAGAGAAACAACGGAGATGAATTTCCCTGTTATCAACATGAACAATCTAAATGGTGAAGACAGAGTATCTGCAATGAAACAGATCAACGATGCTTGTGAAAATTGGGGTTTCTTCGAGGTAGATATATATATGTGTATTTTTTTCTTTTAAATTTATCATAACTCATCAGCTTCTCTTATGAATTTTTTTTTTCTTTTTAAAATGATGAAGTTGGTGAATCATGGGATATCACATGAGCTGATGGACAAAGTGGAGAAGCTGACAAAGGAGCATTATAGAAAGTGTATGGAACAGAGGTTCAAAGAAATGGTAGCGATCAAAGGGTTGGATTCAGTGGAAACTGAGATTAACGACACTGATTGGGAAAGCACTTTTTTTTTACGACATCTTCCAGTTTCCAACATGTCGGAAATTCCTGATCTGGACGAGGATTACAGGCAGGTGATGAAGGAATTTGCAGTAGAATTGGAGAAATTAGCAGAGCAAATTCTGGATTTGCTTTGTGAGAATCTTGGGCTTGAAAAAGGGTACTTGAAAAAGGCGTTTTATGGGTCTAAAGGTCCTAACTTTGGGACTAAAGTTAGCAATTACCCTCCATGTCCTAAACCGGAGCTGATTAAAGGGCTTAGAGCCCATACTGATGCTGGTGGGCTTATTCTTCTGTTTCAAGACGACAAAGTCAGTGGGCTTCAGCTGCTCAAAGATGGCAAATGGGTTGATGTCCCACCAATGCATCACTCTATTGTCATCAATTTAGGAGATCAGCTCGAAGTATGTGCTATGTTAAGTTTTATTTGAAATTATTTCTAAATCCTTACAAAACTAGTTTTATATGCAGTACGTGATCAACATTCATTTCATTTTTAAGTAATACGGCTTGCATTGCCCATCTTCCTACCTTCCCTATATGGAAAAAAAAAAGGATTTGTCACATGAAAAATTAGATTAGCTAAGTATTATTACTATTTGTGTTAACCTTTTGTTTTGTTTTCATTGAAATAGGTTATTACAAATGGAAAGTACAAGAGTGTAATGCACAGAGTGATAGCACAGCAAGATGGAAACAGAATGTCGATAGCATCATTCTACAATCCAGGAAACGACGCCGTAATCTATCCGGCGCCGGCGCTGGTGGAAGGAGAGGAAGAAAAAAGCAAACTTTACCCAAAATTTGTGTTCGACGATTACATGAAGCTGTACGTGGGGCTTAAGTTTCAAGCAAAAGAGCCAAGGTTTGAGGCTATGAAAGCCATGGAGTCTACCAATCTCAATTTGGGTCCAATTGCAACTGTTTGAAAACCCCCCCAAAAAACACAAGAAAGATCAAACAAATTAAAGGGATTTGGATTAATTGTGGGTGTTTTTATCATTATTATTGTTAGAAAAGAACAAATTGTAGTGGTACATTAGTGATGATGTTTGGGGGTTGGGGAACAGTAGTCTGTATTTTTATTTTACTTTTAAAAACATGATAGTGGTTTGGGAGCAATATGAAGGCTTTCTAGGTTAATAGTTATAACAATAATGGATATGTGTGTGTCTTTGTTATTCTGAAATATGGTTTTTATTCAGAGTGGAAATAGACTTACTTTGTATACAAACTTGCATTATCTTGTGT

mRNA sequence

ACCTTTCCCACTATCTTTTTCCTTCCTTAATTTTCCACTTTCTTTTATTTCCCCTTCCCCTATAAATACCAGCCCATCTCCACCACTCAAAACACACACTCAAAACACTAAGAAAGTTACAAAGTTTTTGAGAGAAACAACGGAGATGAATTTCCCTGTTATCAACATGAACAATCTAAATGGTGAAGACAGAGTATCTGCAATGAAACAGATCAACGATGCTTGTGAAAATTGGGGTTTCTTCGAGTTGGTGAATCATGGGATATCACATGAGCTGATGGACAAAGTGGAGAAGCTGACAAAGGAGCATTATAGAAAGTGTATGGAACAGAGGTTCAAAGAAATGGTAGCGATCAAAGGGTTGGATTCAGTGGAAACTGAGATTAACGACACTGATTGGGAAAGCACTTTTTTTTTACGACATCTTCCAGTTTCCAACATGTCGGAAATTCCTGATCTGGACGAGGATTACAGGCAGGTGATGAAGGAATTTGCAGTAGAATTGGAGAAATTAGCAGAGCAAATTCTGGATTTGCTTTGTGAGAATCTTGGGCTTGAAAAAGGGTACTTGAAAAAGGCGTTTTATGGGTCTAAAGGTCCTAACTTTGGGACTAAAGTTAGCAATTACCCTCCATGTCCTAAACCGGAGCTGATTAAAGGGCTTAGAGCCCATACTGATGCTGGTGGGCTTATTCTTCTGTTTCAAGACGACAAAGTCAGTGGGCTTCAGCTGCTCAAAGATGGCAAATGGGTTGATGTCCCACCAATGCATCACTCTATTGTCATCAATTTAGGAGATCAGCTCGAAGTTATTACAAATGGAAAGTACAAGAGTGTAATGCACAGAGTGATAGCACAGCAAGATGGAAACAGAATGTCGATAGCATCATTCTACAATCCAGGAAACGACGCCGTAATCTATCCGGCGCCGGCGCTGGTGGAAGGAGAGGAAGAAAAAAGCAAACTTTACCCAAAATTTGTGTTCGACGATTACATGAAGCTGTACGTGGGGCTTAAGTTTCAAGCAAAAGAGCCAAGGTTTGAGGCTATGAAAGCCATGGAGTCTACCAATCTCAATTTGGGTCCAATTGCAACTGTTTGAAAACCCCCCCAAAAAACACAAGAAAGATCAAACAAATTAAAGGGATTTGGATTAATTGTGGGTGTTTTTATCATTATTATTGTTAGAAAAGAACAAATTGTAGTGGTACATTAGTGATGATGTTTGGGGGTTGGGGAACAGTAGTCTGTATTTTTATTTTACTTTTAAAAACATGATAGTGGTTTGGGAGCAATATGAAGGCTTTCTAGGTTAATAGTTATAACAATAATGGATATGTGTGTGTCTTTGTTATTCTGAAATATGGTTTTTATTCAGAGTGGAAATAGACTTACTTTGTATACAAACTTGCATTATCTTGTGT

Coding sequence (CDS)

ATGAATTTCCCTGTTATCAACATGAACAATCTAAATGGTGAAGACAGAGTATCTGCAATGAAACAGATCAACGATGCTTGTGAAAATTGGGGTTTCTTCGAGTTGGTGAATCATGGGATATCACATGAGCTGATGGACAAAGTGGAGAAGCTGACAAAGGAGCATTATAGAAAGTGTATGGAACAGAGGTTCAAAGAAATGGTAGCGATCAAAGGGTTGGATTCAGTGGAAACTGAGATTAACGACACTGATTGGGAAAGCACTTTTTTTTTACGACATCTTCCAGTTTCCAACATGTCGGAAATTCCTGATCTGGACGAGGATTACAGGCAGGTGATGAAGGAATTTGCAGTAGAATTGGAGAAATTAGCAGAGCAAATTCTGGATTTGCTTTGTGAGAATCTTGGGCTTGAAAAAGGGTACTTGAAAAAGGCGTTTTATGGGTCTAAAGGTCCTAACTTTGGGACTAAAGTTAGCAATTACCCTCCATGTCCTAAACCGGAGCTGATTAAAGGGCTTAGAGCCCATACTGATGCTGGTGGGCTTATTCTTCTGTTTCAAGACGACAAAGTCAGTGGGCTTCAGCTGCTCAAAGATGGCAAATGGGTTGATGTCCCACCAATGCATCACTCTATTGTCATCAATTTAGGAGATCAGCTCGAAGTTATTACAAATGGAAAGTACAAGAGTGTAATGCACAGAGTGATAGCACAGCAAGATGGAAACAGAATGTCGATAGCATCATTCTACAATCCAGGAAACGACGCCGTAATCTATCCGGCGCCGGCGCTGGTGGAAGGAGAGGAAGAAAAAAGCAAACTTTACCCAAAATTTGTGTTCGACGATTACATGAAGCTGTACGTGGGGCTTAAGTTTCAAGCAAAAGAGCCAAGGTTTGAGGCTATGAAAGCCATGGAGTCTACCAATCTCAATTTGGGTCCAATTGCAACTGTTTGA

Protein sequence

MNFPVINMNNLNGEDRVSAMKQINDACENWGFFELVNHGISHELMDKVEKLTKEHYRKCMEQRFKEMVAIKGLDSVETEINDTDWESTFFLRHLPVSNMSEIPDLDEDYRQVMKEFAVELEKLAEQILDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAGGLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQQDGNRMSIASFYNPGNDAVIYPAPALVEGEEEKSKLYPKFVFDDYMKLYVGLKFQAKEPRFEAMKAMESTNLNLGPIATV
BLAST of Bhi03G000482 vs. Swiss-Prot
Match: sp|P54847|ACCO3_CUCME (1-aminocyclopropane-1-carboxylate oxidase 3 OS=Cucumis melo OX=3656 GN=ACO3 PE=2 SV=1)

HSP 1 Score: 622.9 bits (1605), Expect = 2.0e-177
Identity = 298/318 (93.71%), Postives = 310/318 (97.48%), Query Frame = 0

Query: 1   MNFPVINMNNLNGEDRVSAMKQINDACENWGFFELVNHGISHELMDKVEKLTKEHYRKCM 60
           M+FPVINMNNLNGE RVS + QINDACENWGFFELVNHGISHELMDKVEKLTKEHYRKCM
Sbjct: 3   MDFPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGISHELMDKVEKLTKEHYRKCM 62

Query: 61  EQRFKEMVAIKGLDSVETEINDTDWESTFFLRHLPVSNMSEIPDLDEDYRQVMKEFAVEL 120
           EQRFKEMVA KGLDSVETEINDTDWESTFFLRHLPVSNMSEI DLDE+Y++VMKEFA EL
Sbjct: 63  EQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFADEL 122

Query: 121 EKLAEQILDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG 180
           EKLAE++LDLLCENLGLEKGYLKK FYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG
Sbjct: 123 EKLAEEVLDLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG 182

Query: 181 GLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQQD 240
           GLILLFQDDKVSGL +LKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ+D
Sbjct: 183 GLILLFQDDKVSGLHVLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQED 242

Query: 241 GNRMSIASFYNPGNDAVIYPAPALVEGEEEKSKLYPKFVFDDYMKLYVGLKFQAKEPRFE 300
           GNRMSIASFYNPGNDAVIYPAPALVEGE+EK+KLYPKFVFDDYMKLYVGLKFQAKEPRFE
Sbjct: 243 GNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYVGLKFQAKEPRFE 302

Query: 301 AMKAMESTNLNLGPIATV 319
           AMKAMESTNLN+GPIATV
Sbjct: 303 AMKAMESTNLNMGPIATV 320

BLAST of Bhi03G000482 vs. Swiss-Prot
Match: sp|P31237|ACCO_ACTDE (1-aminocyclopropane-1-carboxylate oxidase OS=Actinidia deliciosa OX=3627 GN=ACO PE=2 SV=1)

HSP 1 Score: 555.8 bits (1431), Expect = 3.0e-157
Identity = 263/317 (82.97%), Postives = 296/317 (93.38%), Query Frame = 0

Query: 3   FPVINMNNLNGEDRVSAMKQINDACENWGFFELVNHGISHELMDKVEKLTKEHYRKCMEQ 62
           FPVI+M  LNGE+R   M++I DACENWGFFELVNHGISHELMD VE+LTKEHY KCMEQ
Sbjct: 4   FPVIDMEKLNGEERAPTMEKIKDACENWGFFELVNHGISHELMDTVERLTKEHYNKCMEQ 63

Query: 63  RFKEMVAIKGLDSVETEINDTDWESTFFLRHLPVSNMSEIPDLDEDYRQVMKEFAVELEK 122
           RFKEMVA KGL++V++EIND DWESTFFLRHLPVSN+SEIPDL++D+R+ MKEFA +LEK
Sbjct: 64  RFKEMVATKGLEAVQSEINDLDWESTFFLRHLPVSNISEIPDLEQDHRKAMKEFAEKLEK 123

Query: 123 LAEQILDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAGGL 182
           LAEQ+LDLLCEN+GLEKGYLKKAFYGSKGP FGTKVSNYPPCP+PELIKGLRAHTDAGG+
Sbjct: 124 LAEQLLDLLCENVGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPRPELIKGLRAHTDAGGI 183

Query: 183 ILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQQDGN 242
           ILLFQD+KVSGLQLLKDG+W+DVPPM HSIVIN+GDQLEVITNGKYKSVMHRVIAQ DGN
Sbjct: 184 ILLFQDNKVSGLQLLKDGEWIDVPPMKHSIVINIGDQLEVITNGKYKSVMHRVIAQPDGN 243

Query: 243 RMSIASFYNPGNDAVIYPAPALVEGEEEKSK-LYPKFVFDDYMKLYVGLKFQAKEPRFEA 302
           RMSIASFYNPG+DAV+YPAPALV+ EE++ K +YPKFVF+DYMKLY GLKFQAKEPRFEA
Sbjct: 244 RMSIASFYNPGSDAVMYPAPALVDKEEDQQKQVYPKFVFEDYMKLYAGLKFQAKEPRFEA 303

Query: 303 MKAMESTNLNLGPIATV 319
           MKAME+  +NLGPIAT+
Sbjct: 304 MKAMENA-VNLGPIATI 319

BLAST of Bhi03G000482 vs. Swiss-Prot
Match: sp|Q8S932|ACCO_DIOKA (1-aminocyclopropane-1-carboxylate oxidase OS=Diospyros kaki OX=35925 GN=DK-ACO1 PE=2 SV=1)

HSP 1 Score: 538.9 bits (1387), Expect = 3.8e-152
Identity = 253/317 (79.81%), Postives = 286/317 (90.22%), Query Frame = 0

Query: 2   NFPVINMNNLNGEDRVSAMKQINDACENWGFFELVNHGISHELMDKVEKLTKEHYRKCME 61
           +FPVINM  +NGE+R + M  INDACENWGFFELVNHGI  ELMD VE++TK HY+KCME
Sbjct: 3   SFPVINMEKMNGEERAATMGLINDACENWGFFELVNHGIPPELMDTVERVTKAHYKKCME 62

Query: 62  QRFKEMVAIKGLDSVETEINDTDWESTFFLRHLPVSNMSEIPDLDEDYRQVMKEFAVELE 121
           QRFKE+VA K L+ ++ E+ D DWEST+FLRHLP SN+SE+PDLDE+YR+VMK+FA  LE
Sbjct: 63  QRFKELVASKALEGIQAEVTDMDWESTYFLRHLPQSNISEVPDLDEEYRRVMKDFAERLE 122

Query: 122 KLAEQILDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAGG 181
           KLAE +LDLLCENLGLEKGYLKKAFYG+KGPNFGTKV+NYPPCPK +LIKGLRAHTDAGG
Sbjct: 123 KLAEYLLDLLCENLGLEKGYLKKAFYGTKGPNFGTKVANYPPCPKADLIKGLRAHTDAGG 182

Query: 182 LILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQQDG 241
           +ILLFQDDKVSGLQLLKD +W+DVPPM HSIVINLGDQLEVITNGKYKSV+HRV+AQ DG
Sbjct: 183 IILLFQDDKVSGLQLLKDDQWIDVPPMKHSIVINLGDQLEVITNGKYKSVLHRVVAQTDG 242

Query: 242 NRMSIASFYNPGNDAVIYPAPALVEGEEEKSKLYPKFVFDDYMKLYVGLKFQAKEPRFEA 301
            RMSIASFYNPGNDAVIYPAPALVE E E+ ++YPKFVFDDYMKLY  LKFQAKEPRFEA
Sbjct: 243 TRMSIASFYNPGNDAVIYPAPALVEKEVEEKEVYPKFVFDDYMKLYAALKFQAKEPRFEA 302

Query: 302 MKAMESTNLNLGPIATV 319
           MKA+E+ N+NLGPIATV
Sbjct: 303 MKAVEA-NVNLGPIATV 318

BLAST of Bhi03G000482 vs. Swiss-Prot
Match: sp|Q08506|ACCO1_PETHY (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Petunia hybrida OX=4102 GN=ACO1 PE=1 SV=1)

HSP 1 Score: 538.1 bits (1385), Expect = 6.6e-152
Identity = 251/318 (78.93%), Postives = 289/318 (90.88%), Query Frame = 0

Query: 2   NFPVINMNNLNGEDRVSAMKQINDACENWGFFELVNHGISHELMDKVEKLTKEHYRKCME 61
           NFP+I+++ +NG +R + M+ I DACENWGFFELVNHGI  E+MD VEK+TK HY+KCME
Sbjct: 3   NFPIISLDKVNGVERAATMEMIKDACENWGFFELVNHGIPREVMDTVEKMTKGHYKKCME 62

Query: 62  QRFKEMVAIKGLDSVETEINDTDWESTFFLRHLPVSNMSEIPDLDEDYRQVMKEFAVELE 121
           QRFKE+VA K L+ V+ E+ D DWESTFFL+HLP+SN+SE+PDLDE+YR+VM++FA  LE
Sbjct: 63  QRFKELVASKALEGVQAEVTDMDWESTFFLKHLPISNISEVPDLDEEYREVMRDFAKRLE 122

Query: 122 KLAEQILDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAGG 181
           KLAE++LDLLCENLGLEKGYLK AFYGSKGPNFGTKVSNYPPCPKP+LIKGLRAHTDAGG
Sbjct: 123 KLAEELLDLLCENLGLEKGYLKNAFYGSKGPNFGTKVSNYPPCPKPDLIKGLRAHTDAGG 182

Query: 182 LILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQQDG 241
           +ILLFQDDKVSGLQLLKDG+W+DVPPM HSIV+NLGDQLEVITNGKYKSVMHRVIAQ+DG
Sbjct: 183 IILLFQDDKVSGLQLLKDGQWIDVPPMRHSIVVNLGDQLEVITNGKYKSVMHRVIAQKDG 242

Query: 242 NRMSIASFYNPGNDAVIYPAPALVEGE-EEKSKLYPKFVFDDYMKLYVGLKFQAKEPRFE 301
            RMS+ASFYNPG+DAVIYPAPALVE E EE  ++YPKFVFDDYMKLY GLKFQAKEPRFE
Sbjct: 243 ARMSLASFYNPGSDAVIYPAPALVEKEAEENKQVYPKFVFDDYMKLYAGLKFQAKEPRFE 302

Query: 302 AMKAMESTNLNLGPIATV 319
           AMKAME T++ + PIATV
Sbjct: 303 AMKAME-TDVKMDPIATV 319

BLAST of Bhi03G000482 vs. Swiss-Prot
Match: sp|Q08507|ACCO3_PETHY (1-aminocyclopropane-1-carboxylate oxidase 3 OS=Petunia hybrida OX=4102 GN=ACO3 PE=3 SV=1)

HSP 1 Score: 537.0 bits (1382), Expect = 1.5e-151
Identity = 252/318 (79.25%), Postives = 287/318 (90.25%), Query Frame = 0

Query: 2   NFPVINMNNLNGEDRVSAMKQINDACENWGFFELVNHGISHELMDKVEKLTKEHYRKCME 61
           NFP+IN+  LNG +R + M+ I DACENWGFFELVNHGI HE+MD VEKLTK HY+KCME
Sbjct: 3   NFPIINLEKLNGSERDATMEMIKDACENWGFFELVNHGIPHEVMDTVEKLTKGHYKKCME 62

Query: 62  QRFKEMVAIKGLDSVETEINDTDWESTFFLRHLPVSNMSEIPDLDEDYRQVMKEFAVELE 121
           QRFKE+VA KGL++V+ E+ D DWESTFFLRHLPVSN+SE+PDLD++YR+VM++FA  LE
Sbjct: 63  QRFKELVASKGLEAVQAEVTDLDWESTFFLRHLPVSNISEVPDLDDEYREVMRDFAKRLE 122

Query: 122 KLAEQILDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAGG 181
           KLAE++LDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKP+LIKGLRAHTDAGG
Sbjct: 123 KLAEELLDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPDLIKGLRAHTDAGG 182

Query: 182 LILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQQDG 241
           +ILLFQDDKVSGLQLLKDG+W+DVPPM HSIV+NLGDQLEVITNGKYKSV+HRVIAQ DG
Sbjct: 183 IILLFQDDKVSGLQLLKDGQWIDVPPMRHSIVVNLGDQLEVITNGKYKSVLHRVIAQTDG 242

Query: 242 NRMSIASFYNPGNDAVIYPAPALVEGE--EEKSKLYPKFVFDDYMKLYVGLKFQAKEPRF 301
            RMS+ASFYNPG+DAVIYPAP LVE E  +E  ++YPKFVFDDYMKLY GLKFQAKEPRF
Sbjct: 243 TRMSLASFYNPGSDAVIYPAPTLVEKEADQECKQVYPKFVFDDYMKLYAGLKFQAKEPRF 302

Query: 302 EAMKAMESTNLNLGPIAT 318
           EAMKA E+ ++   PIAT
Sbjct: 303 EAMKAREA-DVKSDPIAT 319

BLAST of Bhi03G000482 vs. TAIR10
Match: AT1G05010.1 (ethylene-forming enzyme)

HSP 1 Score: 510.8 bits (1314), Expect = 6.2e-145
Identity = 237/320 (74.06%), Postives = 285/320 (89.06%), Query Frame = 0

Query: 2   NFPVINMNNLNGEDRVSAMKQINDACENWGFFELVNHGISHELMDKVEKLTKEHYRKCME 61
           +FP+IN+  LNGE+R   M++I DACENWGFFE VNHGIS EL+DKVEK+TKEHY+KCME
Sbjct: 3   SFPIINLEKLNGEERAITMEKIKDACENWGFFECVNHGISLELLDKVEKMTKEHYKKCME 62

Query: 62  QRFKEMVAIKGLDSVETEINDTDWESTFFLRHLPVSNMSEIPDLDEDYRQVMKEFAVELE 121
           +RFKE +  +GLDS+ +E+ND DWESTF+L+HLPVSN+S++PDLD+DYR +MK+FA ++E
Sbjct: 63  ERFKESIKNRGLDSLRSEVNDVDWESTFYLKHLPVSNISDVPDLDDDYRTLMKDFAGKIE 122

Query: 122 KLAEQILDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAGG 181
           KL+E++LDLLCENLGLEKGYLKK FYGSK P FGTKVSNYPPCP P+L+KGLRAHTDAGG
Sbjct: 123 KLSEELLDLLCENLGLEKGYLKKVFYGSKRPTFGTKVSNYPPCPNPDLVKGLRAHTDAGG 182

Query: 182 LILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQQDG 241
           +ILLFQDDKVSGLQLLKDG+WVDVPP+ HSIV+NLGDQLEVITNGKYKSV HRV++Q DG
Sbjct: 183 IILLFQDDKVSGLQLLKDGEWVDVPPVKHSIVVNLGDQLEVITNGKYKSVEHRVLSQTDG 242

Query: 242 -NRMSIASFYNPGNDAVIYPAPALV--EGEEEKSKLYPKFVFDDYMKLYVGLKFQAKEPR 301
             RMSIASFYNPG+D+VI+PAP L+  E E+EK + YP+FVF+DYMKLY  +KFQAKEPR
Sbjct: 243 EGRMSIASFYNPGSDSVIFPAPELIGKEAEKEKKENYPRFVFEDYMKLYSAVKFQAKEPR 302

Query: 302 FEAMKAMESTNL-NLGPIAT 318
           FEAMKAME+T   N+GP+AT
Sbjct: 303 FEAMKAMETTVANNVGPLAT 322

BLAST of Bhi03G000482 vs. TAIR10
Match: AT1G62380.1 (ACC oxidase 2)

HSP 1 Score: 482.6 bits (1241), Expect = 1.8e-136
Identity = 221/318 (69.50%), Postives = 266/318 (83.65%), Query Frame = 0

Query: 1   MNFPVINMNNLNGEDRVSAMKQINDACENWGFFELVNHGISHELMDKVEKLTKEHYRKCM 60
           M FPV++++ LNGE+R   M  IN+ACENWGFFE+VNHG+ H+LMDK+EK+TK+HY+ C 
Sbjct: 5   MKFPVVDLSKLNGEERDQTMALINEACENWGFFEIVNHGLPHDLMDKIEKMTKDHYKTCQ 64

Query: 61  EQRFKEMVAIKGLDSVETEINDTDWESTFFLRHLPVSNMSEIPDLDEDYRQVMKEFAVEL 120
           EQ+F +M+  KGLD++ETE+ D DWESTF++RHLP SN+++I D+ ++YR  MK+F   L
Sbjct: 65  EQKFNDMLKSKGLDNLETEVEDVDWESTFYVRHLPQSNLNDISDVSDEYRTAMKDFGKRL 124

Query: 121 EKLAEQILDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG 180
           E LAE +LDLLCENLGLEKGYLKK F+G+KGP FGTKVSNYPPCPKPE+IKGLRAHTDAG
Sbjct: 125 ENLAEDLLDLLCENLGLEKGYLKKVFHGTKGPTFGTKVSNYPPCPKPEMIKGLRAHTDAG 184

Query: 181 GLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQQD 240
           G+ILLFQDDKVSGLQLLKDG W+DVPP++HSIVINLGDQLEVITNGKYKSV+HRV+ QQ+
Sbjct: 185 GIILLFQDDKVSGLQLLKDGDWIDVPPLNHSIVINLGDQLEVITNGKYKSVLHRVVTQQE 244

Query: 241 GNRMSIASFYNPGNDAVIYPAPALVEGEEEKSKLYPKFVFDDYMKLYVGLKFQAKEPRFE 300
           GNRMS+ASFYNPG+DA I PA +LVE + E    YP FVFDDYMKLY G+KFQ KEPRF 
Sbjct: 245 GNRMSVASFYNPGSDAEISPATSLVEKDSE----YPSFVFDDYMKLYAGVKFQPKEPRFA 304

Query: 301 AMKAMESTNLNLGPIATV 319
           AMK   +    L P A V
Sbjct: 305 AMKNASAVT-ELNPTAAV 317

BLAST of Bhi03G000482 vs. TAIR10
Match: AT1G12010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 479.9 bits (1234), Expect = 1.2e-135
Identity = 226/318 (71.07%), Postives = 267/318 (83.96%), Query Frame = 0

Query: 1   MNFPVINMNNLNGEDRVSAMKQINDACENWGFFELVNHGISHELMDKVEKLTKEHYRKCM 60
           + FPVI+++ LNGE+R   M  I+DAC+NWGFFELVNHG+ ++LMD +E++TKEHY+K M
Sbjct: 5   IKFPVIDLSKLNGEERDQTMALIDDACQNWGFFELVNHGLPYDLMDNIERMTKEHYKKHM 64

Query: 61  EQRFKEMVAIKGLDSVETEINDTDWESTFFLRHLPVSNMSEIPDLDEDYRQVMKEFAVEL 120
           EQ+FKEM+  KGLD++ETE+ D DWESTF+L HLP SN+ +IPD+  +YR  MK+F   L
Sbjct: 65  EQKFKEMLRSKGLDTLETEVEDVDWESTFYLHHLPQSNLYDIPDMSNEYRLAMKDFGKRL 124

Query: 121 EKLAEQILDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG 180
           E LAE++LDLLCENLGLEKGYLKK F+G+ GP F TK+SNYPPCPKPE+IKGLRAHTDAG
Sbjct: 125 EILAEELLDLLCENLGLEKGYLKKVFHGTTGPTFATKLSNYPPCPKPEMIKGLRAHTDAG 184

Query: 181 GLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQQD 240
           GLILLFQDDKVSGLQLLKDG WVDVPP+ HSIVINLGDQLEVITNGKYKSVMHRV+ Q++
Sbjct: 185 GLILLFQDDKVSGLQLLKDGDWVDVPPLKHSIVINLGDQLEVITNGKYKSVMHRVMTQKE 244

Query: 241 GNRMSIASFYNPGNDAVIYPAPALVEGEEEKSKLYPKFVFDDYMKLYVGLKFQAKEPRFE 300
           GNRMSIASFYNPG+DA I PA +LV    +K   YP FVFDDYMKLY GLKFQAKEPRFE
Sbjct: 245 GNRMSIASFYNPGSDAEISPATSLV----DKDSKYPSFVFDDYMKLYAGLKFQAKEPRFE 304

Query: 301 AMKAMESTNLNLGPIATV 319
           AMK  E+   +L P+A V
Sbjct: 305 AMKNAEAA-ADLNPVAVV 317

BLAST of Bhi03G000482 vs. TAIR10
Match: AT1G77330.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 313.5 bits (802), Expect = 1.5e-85
Identity = 159/315 (50.48%), Postives = 215/315 (68.25%), Query Frame = 0

Query: 1   MNFPVINMNNLNGEDRVSAMKQINDACENWGFFELVNHGISHELMDKVEKLTKEHYRKCM 60
           M  PVI+ + LNGE+R   + +I  ACE WGFF+LVNHGI  EL++KV+KL+ + Y+   
Sbjct: 1   MAIPVIDFSKLNGEEREKTLSEIARACEEWGFFQLVNHGIPLELLNKVKKLSSDCYKTER 60

Query: 61  EQRFKEMVAIKGLDSV-----ETEINDTDWESTFFLRHLPVSNMSEIPDLDEDYRQVMKE 120
           E+ FK    +K L+ +       ++ + DWE  F L      N +E P    + ++ M E
Sbjct: 61  EEAFKTSNPVKLLNELVQKNSGEKLENVDWEDVFTLLD---HNQNEWP---SNIKETMGE 120

Query: 121 FAVELEKLAEQILDLLCENLGLEKGYLKKAFY-----GSKGPNFGTKVSNYPPCPKPELI 180
           +  E+ KLA ++++++ ENLGL KGY+KKAF      G +   FGTKVS+YPPCP PEL+
Sbjct: 121 YREEVRKLASKMMEVMDENLGLPKGYIKKAFNEGMEDGEETAFFGTKVSHYPPCPHPELV 180

Query: 181 KGLRAHTDAGGLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKS 240
            GLRAHTDAGG++LLFQDD+  GLQ+LKDG+W+DV P+ ++IVIN GDQ+EV++NG+YKS
Sbjct: 181 NGLRAHTDAGGVVLLFQDDEYDGLQVLKDGEWIDVQPLPNAIVINTGDQIEVLSNGRYKS 240

Query: 241 VMHRVIAQQDGNRMSIASFYNPGNDAVIYPAPALVEGEEEKSKLYPKFVFDDYMKLYVGL 300
             HRV+A+++GNR SIASFYNP   A I   PA V  EE   K YPKFVF DYM +Y   
Sbjct: 241 AWHRVLAREEGNRRSIASFYNPSYKAAI--GPAAVAEEEGSEKKYPKFVFGDYMDVYANQ 300

Query: 301 KFQAKEPRFEAMKAM 306
           KF  KEPRF A+K++
Sbjct: 301 KFMPKEPRFLAVKSL 307

BLAST of Bhi03G000482 vs. TAIR10
Match: AT2G19590.1 (ACC oxidase 1)

HSP 1 Score: 297.0 bits (759), Expect = 1.4e-80
Identity = 145/307 (47.23%), Postives = 199/307 (64.82%), Query Frame = 0

Query: 1   MNFPVINMNNLNGEDRVSAMKQINDACENWGFFELVNHGISHELMDKVEKLTKEHYRKCM 60
           M  PVI+   L+GE R   M  ++ AC+ WGFF + NHGI  ELM+KV+K+   HY + +
Sbjct: 9   MEIPVIDFAELDGEKRSKTMSLLDHACDKWGFFMVDNHGIDKELMEKVKKMINSHYEEHL 68

Query: 61  EQRFKEMVAIKGLDSVETEINDTDWESTFFLRHLPVSNMSEIPDLDEDYRQVMKEFAVEL 120
           +++F +   +K L   E + +D DWES+FF+ H P SN+ +IP++ E+  + M E     
Sbjct: 69  KEKFYQSEMVKALS--EGKTSDADWESSFFISHKPTSNICQIPNISEELSKTMDEXXXXX 128

Query: 121 EKLAEQILDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG 180
                        NLGL++  +  AF G KGP FGTKV+ YP CP+PEL++GLR HTDAG
Sbjct: 129 XXXXXXXXXXXXXNLGLDQEDIMNAFSGPKGPAFGTKVAKYPECPRPELMRGLREHTDAG 188

Query: 181 GLILLFQDDKVSGLQLLKDGKWVDVPP-MHHSIVINLGDQLEVITNGKYKSVMHRVIAQQ 240
           G+ILL QDD+V GL+  KDGKWV +PP  +++I +N GDQLE+++NG+YKSV+HRV+  +
Sbjct: 189 GIILLLQDDQVPGLEFFKDGKWVPIPPSKNNTIFVNTGDQLEILSNGRYKSVVHRVMTVK 248

Query: 241 DGNRMSIASFYNPGNDAVIYPAPALVEGEEEKSKLYPK-FVFDDYMKLYVGLKFQAKEPR 300
            G+R+SIA+FYNP  DA+I PAP L         LYP  + F DY+KLY   KF  K PR
Sbjct: 249 HGSRLSIATFYNPAGDAIISPAPKL---------LYPSGYRFQDYLKLYSTTKFGDKGPR 304

Query: 301 FEAMKAM 306
            E MK M
Sbjct: 309 LETMKKM 304

BLAST of Bhi03G000482 vs. TrEMBL
Match: tr|A0A1S3B069|A0A1S3B069_CUCME (1-aminocyclopropane-1-carboxylate oxidase 3 OS=Cucumis melo OX=3656 GN=LOC103484645 PE=3 SV=1)

HSP 1 Score: 625.9 bits (1613), Expect = 4.8e-176
Identity = 300/318 (94.34%), Postives = 311/318 (97.80%), Query Frame = 0

Query: 1   MNFPVINMNNLNGEDRVSAMKQINDACENWGFFELVNHGISHELMDKVEKLTKEHYRKCM 60
           M+FPVINMNNLNGE RVS + QINDACENWGFFELVNHGISHELMDKVEKLTKEHYRKCM
Sbjct: 3   MDFPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGISHELMDKVEKLTKEHYRKCM 62

Query: 61  EQRFKEMVAIKGLDSVETEINDTDWESTFFLRHLPVSNMSEIPDLDEDYRQVMKEFAVEL 120
           EQRFKEMVA KGLDSVETEINDTDWESTFFLRHLPVSNMSEI DLDE+Y++VMKEFA EL
Sbjct: 63  EQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFADEL 122

Query: 121 EKLAEQILDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG 180
           EKLAE++LDLLCENLGLEKGYLKK FYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG
Sbjct: 123 EKLAEEVLDLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG 182

Query: 181 GLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQQD 240
           GLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ+D
Sbjct: 183 GLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQED 242

Query: 241 GNRMSIASFYNPGNDAVIYPAPALVEGEEEKSKLYPKFVFDDYMKLYVGLKFQAKEPRFE 300
           GNRMSIASFYNPGNDAVIYPAPALVEGE+EK+KLYPKFVFDDYMKLYVGLKFQAKEPRFE
Sbjct: 243 GNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYVGLKFQAKEPRFE 302

Query: 301 AMKAMESTNLNLGPIATV 319
           AMKAMESTNLN+GPIATV
Sbjct: 303 AMKAMESTNLNMGPIATV 320

BLAST of Bhi03G000482 vs. TrEMBL
Match: tr|A0A0A0KH90|A0A0A0KH90_CUCSA (ACC oxidase 2 OS=Cucumis sativus OX=3659 GN=Csa_6G511860 PE=3 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 1.6e-174
Identity = 296/318 (93.08%), Postives = 310/318 (97.48%), Query Frame = 0

Query: 1   MNFPVINMNNLNGEDRVSAMKQINDACENWGFFELVNHGISHELMDKVEKLTKEHYRKCM 60
           M+FPVINMNNLNGE RVS + QINDACENWGFFELVNHGI HELMDKVEK+TKEHYRKCM
Sbjct: 3   MDFPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGIPHELMDKVEKMTKEHYRKCM 62

Query: 61  EQRFKEMVAIKGLDSVETEINDTDWESTFFLRHLPVSNMSEIPDLDEDYRQVMKEFAVEL 120
           EQRFKEMVA KGLDSVETEINDTDWESTFFLRHLPVSNMSEI DLDE+Y++VMKEFA EL
Sbjct: 63  EQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFAAEL 122

Query: 121 EKLAEQILDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG 180
           EKLAE+IL+LLCENLGLEKGYLKK FYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG
Sbjct: 123 EKLAEEILNLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG 182

Query: 181 GLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQQD 240
           GLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ+D
Sbjct: 183 GLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQED 242

Query: 241 GNRMSIASFYNPGNDAVIYPAPALVEGEEEKSKLYPKFVFDDYMKLYVGLKFQAKEPRFE 300
           GNRMSIASFYNPGNDAVIYPAPALVEGE+EK+KLYPKFVFDDYMKLY+GLKFQAKEPRFE
Sbjct: 243 GNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYMGLKFQAKEPRFE 302

Query: 301 AMKAMESTNLNLGPIATV 319
           AMKAMESTN+N+GPIATV
Sbjct: 303 AMKAMESTNINMGPIATV 320

BLAST of Bhi03G000482 vs. TrEMBL
Match: tr|O64946|O64946_CUCSA (ACC oxidase 2 OS=Cucumis sativus OX=3659 GN=Cs-ACO2 PE=2 SV=2)

HSP 1 Score: 616.3 bits (1588), Expect = 3.8e-173
Identity = 294/318 (92.45%), Postives = 308/318 (96.86%), Query Frame = 0

Query: 1   MNFPVINMNNLNGEDRVSAMKQINDACENWGFFELVNHGISHELMDKVEKLTKEHYRKCM 60
           M+FPVINMNNLNGE RVS + QIND CENWGFFELVNHGI HELMDKVEK+TKEHYRKCM
Sbjct: 1   MDFPVINMNNLNGESRVSFLNQINDVCENWGFFELVNHGIPHELMDKVEKMTKEHYRKCM 60

Query: 61  EQRFKEMVAIKGLDSVETEINDTDWESTFFLRHLPVSNMSEIPDLDEDYRQVMKEFAVEL 120
           EQRFKEMVA KGLDSVETEINDTDWESTFFLRHLPVSNMSEI DLDE+Y++VMKEFA EL
Sbjct: 61  EQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFAAEL 120

Query: 121 EKLAEQILDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG 180
           EKLA +IL+LLCENLGLEKGYLKK FYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG
Sbjct: 121 EKLAXEILNLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG 180

Query: 181 GLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQQD 240
           GLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ+D
Sbjct: 181 GLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQED 240

Query: 241 GNRMSIASFYNPGNDAVIYPAPALVEGEEEKSKLYPKFVFDDYMKLYVGLKFQAKEPRFE 300
           GNRMSIASFYNPGNDAVIYPAPALVEGE+EK+KLYPKFVFDDYMKLY+GLKFQAKEPRFE
Sbjct: 241 GNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYMGLKFQAKEPRFE 300

Query: 301 AMKAMESTNLNLGPIATV 319
           AMKAMESTN+N+GPIATV
Sbjct: 301 AMKAMESTNINMGPIATV 318

BLAST of Bhi03G000482 vs. TrEMBL
Match: tr|A0A2N9EQL5|A0A2N9EQL5_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS5014 PE=3 SV=1)

HSP 1 Score: 568.2 bits (1463), Expect = 1.2e-158
Identity = 268/316 (84.81%), Postives = 295/316 (93.35%), Query Frame = 0

Query: 3   FPVINMNNLNGEDRVSAMKQINDACENWGFFELVNHGISHELMDKVEKLTKEHYRKCMEQ 62
           FPVI++  LNGE+R + M+ + DACENWGFFELVNHGISH+LMD VEKLTKEHYRKCMEQ
Sbjct: 4   FPVIDIEKLNGEERGTVMELVKDACENWGFFELVNHGISHDLMDTVEKLTKEHYRKCMEQ 63

Query: 63  RFKEMVAIKGLDSVETEINDTDWESTFFLRHLPVSNMSEIPDLDEDYRQVMKEFAVELEK 122
           RFKEMV  KGL++V++EIND DWESTFFLRHLPVSN+SEIPDL++DYR+ MKEFA +LEK
Sbjct: 64  RFKEMVESKGLEAVQSEINDMDWESTFFLRHLPVSNLSEIPDLEDDYRKAMKEFAFQLEK 123

Query: 123 LAEQILDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAGGL 182
           LAEQ+LDLLCENLGLEKGYLKKAFYGSKGP FGTKVSNYPPCPKPELIKGLRAHTDAGGL
Sbjct: 124 LAEQLLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPELIKGLRAHTDAGGL 183

Query: 183 ILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQQDGN 242
           ILLFQDDKVSGLQL KDGKW+DVPPMHHSIVIN+GDQLEVITNGKYKSV+HRV+AQ DGN
Sbjct: 184 ILLFQDDKVSGLQLFKDGKWIDVPPMHHSIVINIGDQLEVITNGKYKSVIHRVVAQPDGN 243

Query: 243 RMSIASFYNPGNDAVIYPAPALVEGEEEKSKLYPKFVFDDYMKLYVGLKFQAKEPRFEAM 302
           RMSIASFYNPG+DAVIYPAPALVE E EK ++YPKFVF+DYMKLY GLKFQAKEPRFEAM
Sbjct: 244 RMSIASFYNPGSDAVIYPAPALVEKEAEKGQVYPKFVFEDYMKLYAGLKFQAKEPRFEAM 303

Query: 303 KAMESTNLNLGPIATV 319
           KA+EST +NLGPI TV
Sbjct: 304 KALEST-INLGPIPTV 318

BLAST of Bhi03G000482 vs. TrEMBL
Match: tr|A0A0D2S3H9|A0A0D2S3H9_GOSRA (Uncharacterized protein OS=Gossypium raimondii OX=29730 GN=B456_009G182300 PE=3 SV=1)

HSP 1 Score: 566.6 bits (1459), Expect = 3.5e-158
Identity = 270/318 (84.91%), Postives = 296/318 (93.08%), Query Frame = 0

Query: 1   MNFPVINMNNLNGEDRVSAMKQINDACENWGFFELVNHGISHELMDKVEKLTKEHYRKCM 60
           + FPVI+ + LNGE+R +AM+ I DACENWGFFELVNHGISHELMD VE+LTKEHYRKCM
Sbjct: 3   LTFPVIDFSKLNGEERAAAMEMIKDACENWGFFELVNHGISHELMDTVERLTKEHYRKCM 62

Query: 61  EQRFKEMVAIKGLDSVETEINDTDWESTFFLRHLPVSNMSEIPDLDEDYRQVMKEFAVEL 120
           EQRFKEMVA KGL++V++EIND DWESTFFLRHLP SNMSEIPDL+EDYR+VMKEFAVEL
Sbjct: 63  EQRFKEMVASKGLEAVQSEINDMDWESTFFLRHLPESNMSEIPDLEEDYRKVMKEFAVEL 122

Query: 121 EKLAEQILDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG 180
           EKLAEQ+LDLLCENLGLE GYLKK FYGSKGP FGTKVSNYPPCPKP+LIKGLRAHTDAG
Sbjct: 123 EKLAEQLLDLLCENLGLEPGYLKKVFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAG 182

Query: 181 GLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQQD 240
           G+ILLFQDDKVSGLQLLKDG+W+DVPPM HSIVINLGDQLEVITNGKYKSVMHRVIAQ D
Sbjct: 183 GIILLFQDDKVSGLQLLKDGQWIDVPPMKHSIVINLGDQLEVITNGKYKSVMHRVIAQTD 242

Query: 241 GNRMSIASFYNPGNDAVIYPAPALVEGEEEKSKLYPKFVFDDYMKLYVGLKFQAKEPRFE 300
           G RMSIASFYNPG+DAVIYPAPAL+E E +KS++YPKFVF+DYMKLY GLKFQAKEPRFE
Sbjct: 243 GTRMSIASFYNPGSDAVIYPAPALLEKEADKSQVYPKFVFEDYMKLYAGLKFQAKEPRFE 302

Query: 301 AMKAMESTNLNLGPIATV 319
           AMK +ES  +NLGPIATV
Sbjct: 303 AMKTVESA-VNLGPIATV 319

BLAST of Bhi03G000482 vs. NCBI nr
Match: XP_008440054.1 (PREDICTED: 1-aminocyclopropane-1-carboxylate oxidase 3 [Cucumis melo])

HSP 1 Score: 625.9 bits (1613), Expect = 7.3e-176
Identity = 300/318 (94.34%), Postives = 311/318 (97.80%), Query Frame = 0

Query: 1   MNFPVINMNNLNGEDRVSAMKQINDACENWGFFELVNHGISHELMDKVEKLTKEHYRKCM 60
           M+FPVINMNNLNGE RVS + QINDACENWGFFELVNHGISHELMDKVEKLTKEHYRKCM
Sbjct: 3   MDFPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGISHELMDKVEKLTKEHYRKCM 62

Query: 61  EQRFKEMVAIKGLDSVETEINDTDWESTFFLRHLPVSNMSEIPDLDEDYRQVMKEFAVEL 120
           EQRFKEMVA KGLDSVETEINDTDWESTFFLRHLPVSNMSEI DLDE+Y++VMKEFA EL
Sbjct: 63  EQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFADEL 122

Query: 121 EKLAEQILDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG 180
           EKLAE++LDLLCENLGLEKGYLKK FYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG
Sbjct: 123 EKLAEEVLDLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG 182

Query: 181 GLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQQD 240
           GLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ+D
Sbjct: 183 GLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQED 242

Query: 241 GNRMSIASFYNPGNDAVIYPAPALVEGEEEKSKLYPKFVFDDYMKLYVGLKFQAKEPRFE 300
           GNRMSIASFYNPGNDAVIYPAPALVEGE+EK+KLYPKFVFDDYMKLYVGLKFQAKEPRFE
Sbjct: 243 GNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYVGLKFQAKEPRFE 302

Query: 301 AMKAMESTNLNLGPIATV 319
           AMKAMESTNLN+GPIATV
Sbjct: 303 AMKAMESTNLNMGPIATV 320

BLAST of Bhi03G000482 vs. NCBI nr
Match: P54847.1 (RecName: Full=1-aminocyclopropane-1-carboxylate oxidase 3; Short=ACC oxidase 3; AltName: Full=Ethylene-forming enzyme; Short=EFE >CAA64799.1 ACC oxidase [Cucumis melo])

HSP 1 Score: 622.9 bits (1605), Expect = 6.2e-175
Identity = 298/318 (93.71%), Postives = 310/318 (97.48%), Query Frame = 0

Query: 1   MNFPVINMNNLNGEDRVSAMKQINDACENWGFFELVNHGISHELMDKVEKLTKEHYRKCM 60
           M+FPVINMNNLNGE RVS + QINDACENWGFFELVNHGISHELMDKVEKLTKEHYRKCM
Sbjct: 3   MDFPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGISHELMDKVEKLTKEHYRKCM 62

Query: 61  EQRFKEMVAIKGLDSVETEINDTDWESTFFLRHLPVSNMSEIPDLDEDYRQVMKEFAVEL 120
           EQRFKEMVA KGLDSVETEINDTDWESTFFLRHLPVSNMSEI DLDE+Y++VMKEFA EL
Sbjct: 63  EQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFADEL 122

Query: 121 EKLAEQILDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG 180
           EKLAE++LDLLCENLGLEKGYLKK FYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG
Sbjct: 123 EKLAEEVLDLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG 182

Query: 181 GLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQQD 240
           GLILLFQDDKVSGL +LKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ+D
Sbjct: 183 GLILLFQDDKVSGLHVLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQED 242

Query: 241 GNRMSIASFYNPGNDAVIYPAPALVEGEEEKSKLYPKFVFDDYMKLYVGLKFQAKEPRFE 300
           GNRMSIASFYNPGNDAVIYPAPALVEGE+EK+KLYPKFVFDDYMKLYVGLKFQAKEPRFE
Sbjct: 243 GNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYVGLKFQAKEPRFE 302

Query: 301 AMKAMESTNLNLGPIATV 319
           AMKAMESTNLN+GPIATV
Sbjct: 303 AMKAMESTNLNMGPIATV 320

BLAST of Bhi03G000482 vs. NCBI nr
Match: KGN49070.1 (ACC oxidase 2 [Cucumis sativus])

HSP 1 Score: 620.9 bits (1600), Expect = 2.4e-174
Identity = 296/318 (93.08%), Postives = 310/318 (97.48%), Query Frame = 0

Query: 1   MNFPVINMNNLNGEDRVSAMKQINDACENWGFFELVNHGISHELMDKVEKLTKEHYRKCM 60
           M+FPVINMNNLNGE RVS + QINDACENWGFFELVNHGI HELMDKVEK+TKEHYRKCM
Sbjct: 3   MDFPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGIPHELMDKVEKMTKEHYRKCM 62

Query: 61  EQRFKEMVAIKGLDSVETEINDTDWESTFFLRHLPVSNMSEIPDLDEDYRQVMKEFAVEL 120
           EQRFKEMVA KGLDSVETEINDTDWESTFFLRHLPVSNMSEI DLDE+Y++VMKEFA EL
Sbjct: 63  EQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFAAEL 122

Query: 121 EKLAEQILDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG 180
           EKLAE+IL+LLCENLGLEKGYLKK FYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG
Sbjct: 123 EKLAEEILNLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG 182

Query: 181 GLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQQD 240
           GLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ+D
Sbjct: 183 GLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQED 242

Query: 241 GNRMSIASFYNPGNDAVIYPAPALVEGEEEKSKLYPKFVFDDYMKLYVGLKFQAKEPRFE 300
           GNRMSIASFYNPGNDAVIYPAPALVEGE+EK+KLYPKFVFDDYMKLY+GLKFQAKEPRFE
Sbjct: 243 GNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYMGLKFQAKEPRFE 302

Query: 301 AMKAMESTNLNLGPIATV 319
           AMKAMESTN+N+GPIATV
Sbjct: 303 AMKAMESTNINMGPIATV 320

BLAST of Bhi03G000482 vs. NCBI nr
Match: NP_001292672.1 (1-aminocyclopropane-1-carboxylate oxidase 3 [Cucumis sativus] >AAC67233.1 ACC oxidase 2 [Cucumis sativus])

HSP 1 Score: 616.3 bits (1588), Expect = 5.8e-173
Identity = 294/318 (92.45%), Postives = 308/318 (96.86%), Query Frame = 0

Query: 1   MNFPVINMNNLNGEDRVSAMKQINDACENWGFFELVNHGISHELMDKVEKLTKEHYRKCM 60
           M+FPVINMNNLNGE RVS + QIND CENWGFFELVNHGI HELMDKVEK+TKEHYRKCM
Sbjct: 1   MDFPVINMNNLNGESRVSFLNQINDVCENWGFFELVNHGIPHELMDKVEKMTKEHYRKCM 60

Query: 61  EQRFKEMVAIKGLDSVETEINDTDWESTFFLRHLPVSNMSEIPDLDEDYRQVMKEFAVEL 120
           EQRFKEMVA KGLDSVETEINDTDWESTFFLRHLPVSNMSEI DLDE+Y++VMKEFA EL
Sbjct: 61  EQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFAAEL 120

Query: 121 EKLAEQILDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG 180
           EKLA +IL+LLCENLGLEKGYLKK FYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG
Sbjct: 121 EKLAXEILNLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG 180

Query: 181 GLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQQD 240
           GLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ+D
Sbjct: 181 GLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQED 240

Query: 241 GNRMSIASFYNPGNDAVIYPAPALVEGEEEKSKLYPKFVFDDYMKLYVGLKFQAKEPRFE 300
           GNRMSIASFYNPGNDAVIYPAPALVEGE+EK+KLYPKFVFDDYMKLY+GLKFQAKEPRFE
Sbjct: 241 GNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYMGLKFQAKEPRFE 300

Query: 301 AMKAMESTNLNLGPIATV 319
           AMKAMESTN+N+GPIATV
Sbjct: 301 AMKAMESTNINMGPIATV 318

BLAST of Bhi03G000482 vs. NCBI nr
Match: XP_022926301.1 (1-aminocyclopropane-1-carboxylate oxidase 3 [Cucurbita moschata])

HSP 1 Score: 607.8 bits (1566), Expect = 2.1e-170
Identity = 291/318 (91.51%), Postives = 307/318 (96.54%), Query Frame = 0

Query: 1   MNFPVINMNNLNGEDRVSAMKQINDACENWGFFELVNHGISHELMDKVEKLTKEHYRKCM 60
           M+FPVINM  LNGEDR +AM+QINDACENWGFFELVNHGISHELMD+VEKLTKEHYR+CM
Sbjct: 19  MDFPVINMEKLNGEDRGAAMEQINDACENWGFFELVNHGISHELMDEVEKLTKEHYRQCM 78

Query: 61  EQRFKEMVAIKGLDSVETEINDTDWESTFFLRHLPVSNMSEIPDLDEDYRQVMKEFAVEL 120
           EQ+F +MVA +GLDSV+TEINDTDWESTFFLRHLPVSNMSEIPDLDEDYR+VMKEFAVEL
Sbjct: 79  EQKFNDMVASRGLDSVDTEINDTDWESTFFLRHLPVSNMSEIPDLDEDYRKVMKEFAVEL 138

Query: 121 EKLAEQILDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG 180
           EKLAEQ+LDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG
Sbjct: 139 EKLAEQLLDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTDAG 198

Query: 181 GLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQQD 240
           GLILLFQD KVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRV AQ D
Sbjct: 199 GLILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVKAQPD 258

Query: 241 GNRMSIASFYNPGNDAVIYPAPALVEGEEEKSKLYPKFVFDDYMKLYVGLKFQAKEPRFE 300
           GNRMS+ASFYNPG+DAVIYPAPALVEGEEE SKLYPKFVF+DYMKLY+GLKFQAKEPRFE
Sbjct: 259 GNRMSLASFYNPGDDAVIYPAPALVEGEEESSKLYPKFVFNDYMKLYMGLKFQAKEPRFE 318

Query: 301 AMKAMESTNLNLGPIATV 319
           AMKA ESTNL +GPIATV
Sbjct: 319 AMKATESTNLKMGPIATV 336

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
sp|P54847|ACCO3_CUCME2.0e-17793.711-aminocyclopropane-1-carboxylate oxidase 3 OS=Cucumis melo OX=3656 GN=ACO3 PE=2... [more]
sp|P31237|ACCO_ACTDE3.0e-15782.971-aminocyclopropane-1-carboxylate oxidase OS=Actinidia deliciosa OX=3627 GN=ACO ... [more]
sp|Q8S932|ACCO_DIOKA3.8e-15279.811-aminocyclopropane-1-carboxylate oxidase OS=Diospyros kaki OX=35925 GN=DK-ACO1 ... [more]
sp|Q08506|ACCO1_PETHY6.6e-15278.931-aminocyclopropane-1-carboxylate oxidase 1 OS=Petunia hybrida OX=4102 GN=ACO1 P... [more]
sp|Q08507|ACCO3_PETHY1.5e-15179.251-aminocyclopropane-1-carboxylate oxidase 3 OS=Petunia hybrida OX=4102 GN=ACO3 P... [more]
Match NameE-valueIdentityDescription
AT1G05010.16.2e-14574.06ethylene-forming enzyme[more]
AT1G62380.11.8e-13669.50ACC oxidase 2[more]
AT1G12010.11.2e-13571.072-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein[more]
AT1G77330.11.5e-8550.482-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein[more]
AT2G19590.11.4e-8047.23ACC oxidase 1[more]
Match NameE-valueIdentityDescription
tr|A0A1S3B069|A0A1S3B069_CUCME4.8e-17694.341-aminocyclopropane-1-carboxylate oxidase 3 OS=Cucumis melo OX=3656 GN=LOC103484... [more]
tr|A0A0A0KH90|A0A0A0KH90_CUCSA1.6e-17493.08ACC oxidase 2 OS=Cucumis sativus OX=3659 GN=Csa_6G511860 PE=3 SV=1[more]
tr|O64946|O64946_CUCSA3.8e-17392.45ACC oxidase 2 OS=Cucumis sativus OX=3659 GN=Cs-ACO2 PE=2 SV=2[more]
tr|A0A2N9EQL5|A0A2N9EQL5_FAGSY1.2e-15884.81Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS5014 PE=3 SV=1[more]
tr|A0A0D2S3H9|A0A0D2S3H9_GOSRA3.5e-15884.91Uncharacterized protein OS=Gossypium raimondii OX=29730 GN=B456_009G182300 PE=3 ... [more]
Match NameE-valueIdentityDescription
XP_008440054.17.3e-17694.34PREDICTED: 1-aminocyclopropane-1-carboxylate oxidase 3 [Cucumis melo][more]
P54847.16.2e-17593.71RecName: Full=1-aminocyclopropane-1-carboxylate oxidase 3; Short=ACC oxidase 3; ... [more]
KGN49070.12.4e-17493.08ACC oxidase 2 [Cucumis sativus][more]
NP_001292672.15.8e-17392.451-aminocyclopropane-1-carboxylate oxidase 3 [Cucumis sativus] >AAC67233.1 ACC ox... [more]
XP_022926301.12.1e-17091.511-aminocyclopropane-1-carboxylate oxidase 3 [Cucurbita moschata][more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
Vocabulary: INTERPRO
TermDefinition
IPR027443IPNS-like
IPR026992DIOX_N
IPR005123Oxoglu/Fe-dep_dioxygenase
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009693 ethylene biosynthetic process
biological_process GO:0009835 fruit ripening
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0009813 flavonoid biosynthetic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0009815 1-aminocyclopropane-1-carboxylate oxidase activity
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0031418 L-ascorbic acid binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016706 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function GO:0005506 iron ion binding
molecular_function GO:0045486 naringenin 3-dioxygenase activity
molecular_function GO:0016491 oxidoreductase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi03M000482Bhi03M000482mRNA


Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 113..133
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 2..307
NoneNo IPR availablePANTHERPTHR10209:SF381SUBFAMILY NOT NAMEDcoord: 2..307
NoneNo IPR availableSUPERFAMILYSSF51197Clavaminate synthase-likecoord: 1..303
IPR005123Oxoglutarate/iron-dependent dioxygenasePFAMPF031712OG-FeII_Oxycoord: 158..252
e-value: 1.9E-27
score: 95.6
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 152..252
score: 13.153
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 4..94
e-value: 3.8E-14
score: 53.3
IPR027443Isopenicillin N synthase-likeGENE3DG3DSA:2.60.120.330coord: 1..318
e-value: 2.6E-126
score: 423.2