Bhi03G000165 (gene) Wax gourd

BLAST of Bhi03G000165 vs. Swiss-Prot
Match: sp|Q9SVG3|BBE21_ARATH (Berberine bridge enzyme-like 21 OS=Arabidopsis thaliana OX=3702 GN=At4g20840 PE=2 SV=1)

HSP 1 Score: 568.5 bits (1464), Expect = 7.9e-161
Identity = 282/521 (54.13%), Postives = 370/521 (71.02%), Query Frame = 0

Query: 34  FLLILP---SMAVSDQIPSHQTLLKCLSDHSAPSSSPISEVIFFPDNPSYSPVLNSYIRN 93
           FL+ LP   S A       +++ ++C SD +    + I++ +F   NPS+S VL +YIRN
Sbjct: 16  FLVSLPFFSSAAPPSSDSIYESFVQCFSDKTKSPQAQITDNVFSRTNPSFSSVLRAYIRN 75

Query: 94  LRFASPTTPKPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGLSYVSDSTFVIL 153
            RF + +TPKP  IV P    H+ A + C +     ++IRSGGHDY+GLSY+SD  F IL
Sbjct: 76  GRFNTSSTPKPAIIVTPRSDIHVSAAVTCSKSLNFLLKIRSGGHDYEGLSYISDKPFFIL 135

Query: 154 DMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTVGVGGHLSGAGY 213
           DM NLR V+VDI D+SAW+ +GATLGEVY++I EKSK+HGFPAGVCPTVGVGGH+SG GY
Sbjct: 136 DMSNLRDVSVDIADQSAWISAGATLGEVYYRIWEKSKVHGFPAGVCPTVGVGGHISGGGY 195

Query: 214 GNLMRKFGVSVDNVVDALIVDVNGRVLDRASMGEDLFWAIRGGGGASFGVIVSWKFKLVP 273
           GN++RKFG+SVDN++DA IVDVNG++LDR SMGEDLFWAI GGGGASFGV++ +K KLVP
Sbjct: 196 GNMLRKFGLSVDNLIDAKIVDVNGQILDRKSMGEDLFWAISGGGGASFGVVLGYKVKLVP 255

Query: 274 LPETVTVFRTEKTMEEGVVDILYKWQEIADKIDENLFIRVVILPVNKKALKTAKAKFISL 333
           +PETVTVFR EK M+ G VD+++KWQ +  K D NLF+R++I PV +K +KT +A  ++L
Sbjct: 256 VPETVTVFRVEKYMDSGAVDMVHKWQSVGPKTDRNLFLRMLIQPVTRKKVKTVRATVVAL 315

Query: 334 FLGNAQKLFALMSKKLPELGIKVEDCIEMSWIDSVLFWSNYPIGTPLN--VLLERQPKSE 393
           FLG A+++ AL+ K+ PEL +K E+C EM+W  S L+W N    T ++  V L+R     
Sbjct: 316 FLGRAEEVVALLGKEFPELSLKKENCSEMTWFQSALWWDNRVNPTQIDPKVFLDRNLDRA 375

Query: 394 KFLKKKSDYVQEPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPHRAGNK 453
            F K+KSDYV   I +  +E + +KM EL +  L FNPYGGKM+++    TPFPHR+   
Sbjct: 376 NFGKRKSDYVASEIPRDGIESLFKKMTELGKIGLVFNPYGGKMAEVTVNATPFPHRS-KL 435

Query: 454 YKIQYSVTWKEEGDEAADKNVELIREMYEYMTPYVSKSPRSAYLNYRDVDLGVNGNG--- 513
           +KIQYSVTW+E   E     +     +Y +MT +VSK+PR+AYLNYRDVD+GVN +G   
Sbjct: 436 FKIQYSVTWQENSVEIEKGFLNQANVLYSFMTGFVSKNPRNAYLNYRDVDIGVNDHGTNS 495

Query: 514 --HASSWGRKYFKGNFDRLVKVKSMVDPDNFFRYEQSIPCV 545
                 +GRKYF  NFDRLVKVK+  DPDNFFR EQSIP V
Sbjct: 496 YEEGEVYGRKYFGDNFDRLVKVKTAADPDNFFRNEQSIPTV 535

BLAST of Bhi03G000165 vs. Swiss-Prot
Match: sp|F4HV09|BBE14_ARATH (Berberine bridge enzyme-like 14 OS=Arabidopsis thaliana OX=3702 GN=At1g34575 PE=3 SV=1)

HSP 1 Score: 563.9 bits (1452), Expect = 2.0e-159
Identity = 281/521 (53.93%), Postives = 375/521 (71.98%), Query Frame = 0

Query: 30  VLTTFLLILP-SMAVSDQIPSHQTLLKCLSDHSAPSSSPISEVIFFPDNPSYSPVLNSYI 89
           + T FLL++P S A   ++    T  +C++      S PI    +   NP++  +LN+Y+
Sbjct: 10  IFTVFLLLIPTSFAAPPKLKDSFT--QCVTVFK--PSVPIQNFTYTQQNPNFLTILNNYV 69

Query: 90  RNLRFASPTTPKPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGLSYVSDSTFV 149
           RNLR+ + TT KP+ IVA  H +HIQATI C +   L++RIRSGGHDYDG+SY+S   FV
Sbjct: 70  RNLRYFNGTTRKPVAIVAAAHFTHIQATINCAKKLGLQLRIRSGGHDYDGMSYLSTVDFV 129

Query: 150 ILDMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKS-KIHGFPAGVCPTVGVGGHLSG 209
           +LDMFNLR++ +D + ++AWV SGATLGE+Y+ +A KS  + GFPAG+CP +G GGH SG
Sbjct: 130 VLDMFNLRAIEIDPKLDTAWVQSGATLGEIYYNVANKSNNLRGFPAGICPGLGAGGHFSG 189

Query: 210 AGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRASMGEDLFWAIRGGGGASFGVIVSWKFK 269
            GYGN+MRK+G+S+DN++DA IVD N RVLDR+SMGEDLFWA+RGGG ASF V+++WK K
Sbjct: 190 GGYGNMMRKYGLSIDNIIDAKIVDANARVLDRSSMGEDLFWALRGGGAASFCVVLAWKIK 249

Query: 270 LVPLPETVTVFRTEKTMEEGVV--DILYKWQEIADKIDENLFIRVVILPVNKKALKTAKA 329
           LVP+PE VTVF  E     GV+  D+  KWQEIADKID +LFIR+ +   N    KT KA
Sbjct: 250 LVPVPEKVTVFNVETIGNRGVIPTDLAAKWQEIADKIDNDLFIRLTLSSSN----KTVKA 309

Query: 330 KFISLFLGNAQKLFALMSKKLPELGIKVEDCIEMSWIDSVLFWSNYPIGT-PLNVLLERQ 389
            F+ ++LGN++KL  +M+ K PELG+   +CIEM WI+SVLFW + P GT P +V+L R 
Sbjct: 310 SFMGMYLGNSEKLLEIMNAKFPELGLNKTECIEMKWIESVLFWLSIPPGTAPTSVMLNRI 369

Query: 390 PKSEKFLKKKSDYVQEPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPHR 449
           P+ + +LK+KSDYVQ+PISK  LE + + + E +  ++ +NPYGG+MS+IP TET FPHR
Sbjct: 370 PQKQIYLKRKSDYVQKPISKPGLESIFKILSENENVSMAWNPYGGRMSEIPATETAFPHR 429

Query: 450 AGNKYKIQYSVTWKEEGDEAADKNVELIREMYEYMTPYVSKSPRSAYLNYRDVDLGVNGN 509
           AGN +KIQYS  W   G+EAA   +     ++E M+PYVSK+PR A+LNYRD+D+G N N
Sbjct: 430 AGNMFKIQYSSNWFVPGEEAASDCLSQTERVFEAMSPYVSKNPREAFLNYRDIDIGKNLN 489

Query: 510 G---HASSWGRKYFKGNFDRLVKVKSMVDPDNFFRYEQSIP 543
                   +G KYFK NF+RLV+VK+ VDPDN FRYEQSIP
Sbjct: 490 STYEEGKVYGVKYFKNNFERLVQVKTRVDPDNIFRYEQSIP 522

BLAST of Bhi03G000165 vs. Swiss-Prot
Match: sp|Q9SA85|BBE8_ARATH (Berberine bridge enzyme-like 8 OS=Arabidopsis thaliana OX=3702 GN=At1g30700 PE=2 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 2.5e-159
Identity = 280/524 (53.44%), Postives = 379/524 (72.33%), Query Frame = 0

Query: 30  VLTTFLLILPSMAVSDQIPSHQTLLKCLSDHSAPSSSPISEVIFFPDNPSYSPVLNSYIR 89
           +L  F ++    + S    S +T  +CL+ +S P   PIS  IFF  N SYS VL + IR
Sbjct: 6   ILVLFFVVFIWQSSSSSANS-ETFTQCLTSNSDP-KHPISPAIFFSGNGSYSSVLQANIR 65

Query: 90  NLRFASPTTPKPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGLSYV--SDSTF 149
           NLRF + +TPKP  I+A TH SH+QA I C + H L+++IRSGGHDYDGLSYV  S   F
Sbjct: 66  NLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPF 125

Query: 150 VILDMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTVGVGGHLSG 209
            +LDMFNLRSV VD+  ++AWV +GA LGEVY+ I EKSK   +PAG+CPTVGVGGH+SG
Sbjct: 126 FVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISG 185

Query: 210 AGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRASMGEDLFWAIRGGGGASFGVIVSWKFK 269
            GYGN+MRK+G++VDN +DA +VDVNG++LDR  MGEDL+WAI GGGG S+GV++++K  
Sbjct: 186 GGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKIN 245

Query: 270 LVPLPETVTVFRTEKTMEEGVVDILYKWQEIADKIDENLFIRVVILPVN--KKALKTAKA 329
           LV +PE VTVFR  +T+E+   DI+++WQ++A K+ + LFIR VI  VN    + KT + 
Sbjct: 246 LVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTVRT 305

Query: 330 KFISLFLGNAQKLFALMSKKLPELGIKVEDCIEMSWIDSVLFWSNYPIGTPLNVLLERQP 389
            FI++FLG+   L ++++++ PELG+   DC E SWI SVLFW+N  +G+   +LL+R  
Sbjct: 306 TFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSSETLLLQRN- 365

Query: 390 KSEKFLKKKSDYVQEPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPHRA 449
           +   +LK+KSDYV+EPIS+  LE + +KMIEL+ P + FNPYGG+M +I  T TPFP+RA
Sbjct: 366 QPVNYLKRKSDYVREPISRTGLESIWKKMIELEIPTMAFNPYGGEMGRISSTVTPFPYRA 425

Query: 450 GNKYKIQYSVTWKEEGDEAADKNVELIREMYEYMTPYVSKSPRSAYLNYRDVDLGVNG-N 509
           GN +KIQY   W++E     D+ +EL R++Y++MTP+VSK+PR ++ NYRDVDLG+N  N
Sbjct: 426 GNLWKIQYGANWRDE--TLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGINSHN 485

Query: 510 GHASS------WGRKYFKGNFDRLVKVKSMVDPDNFFRYEQSIP 543
           G  SS      +G+KYF GNF+RLVK+K+ VD  NFFR EQSIP
Sbjct: 486 GKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIP 524

BLAST of Bhi03G000165 vs. Swiss-Prot
Match: sp|Q9SVG4|RETOL_ARATH (Berberine bridge enzyme-like 19 OS=Arabidopsis thaliana OX=3702 GN=At4g20830 PE=1 SV=2)

HSP 1 Score: 559.3 bits (1440), Expect = 4.8e-158
Identity = 276/517 (53.38%), Postives = 361/517 (69.83%), Query Frame = 0

Query: 36  LILPSMAVSDQIPSHQTLLKCLSDHSAPSSSPISEVIFFPDNPSYSPVLNSYIRNLRFAS 95
           L LP  + S     + + LKC SD +    S I++ +F   NP++S VL +YIRN RF +
Sbjct: 22  LSLPLSSFSQSNSVYNSFLKCFSDKTKSPQSQITDNVFSQTNPAFSSVLRAYIRNARFNT 81

Query: 96  PTTPKPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGLSYVSDSTFVILDMFNL 155
            +T KP  I+ P   SH+ A + C +     ++IRSGGHDYDGLSY+SD  F ILDM N+
Sbjct: 82  SSTLKPTIIITPRSESHVSAAVTCSKTLNFLLKIRSGGHDYDGLSYISDKPFFILDMSNI 141

Query: 156 RSVAVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTVGVGGHLSGAGYGNLMR 215
           R V+VDI   SAW+ +GATLGEVY++I EKS++HGFPAGVCPTVGVGGHLSG GYGN++R
Sbjct: 142 RDVSVDIASNSAWISAGATLGEVYYRIWEKSRVHGFPAGVCPTVGVGGHLSGGGYGNMVR 201

Query: 216 KFGVSVDNVVDALIVDVNGRVLDRASMGEDLFWAIRGGGGASFGVIVSWKFKLVPLPETV 275
           KFG+SVD V DA IVDVNGRVLDR +MGEDLFWAI GGGG S+GV++ +K KLVP+P  V
Sbjct: 202 KFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGGGGSYGVVLGYKVKLVPVPSVV 261

Query: 276 TVFRTEKTMEEGVVDILYKWQEIADKIDENLFIRVVILPVNKKALKTAKAKFISLFLGNA 335
           TVFR E+ M+ G VD+++KWQ +  K D NLF+R++I PV +K +KT +A  ++LFLG A
Sbjct: 262 TVFRVEQYMDSGAVDMVHKWQSVGPKTDPNLFMRMLIQPVTRKKVKTVRASVVALFLGRA 321

Query: 336 QKLFALMSKKLPELGIKVEDCIEMSWIDSVLFWSNYPIGTPLN--VLLERQPKSEKFLKK 395
            ++ AL+SK+ PELG+K E+C EM+W  S L+W N    T ++  V L+R   +  F K+
Sbjct: 322 DEVVALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDPKVFLDRNLDTSSFGKR 381

Query: 396 KSDYVQEPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPHRAGNKYKIQY 455
           KSDYV   I K  +E + +KMIEL +  L FNPYGGKM+++     PFPHR    +KIQY
Sbjct: 382 KSDYVATAIPKKGIESLFKKMIELGKIGLVFNPYGGKMAEVAVNAKPFPHR-NKLFKIQY 441

Query: 456 SVTWKEEGDEAADKNVELIREMYEYMTPYVSKSPRSAYLNYRDVDLGVNGNG-----HAS 515
           SV WKE   E     +   + +Y +MT +VSK+PRS+Y NYRDVD+GVN +G        
Sbjct: 442 SVNWKENSAEIEKGYLNQAKVLYSFMTGFVSKNPRSSYFNYRDVDIGVNDHGANSYKEGE 501

Query: 516 SWGRKYFKGNFDRLVKVKSMVDPDNFFRYEQSIPCVE 546
            +GRKYF  NFDRLVK+K+ VDP NFFR EQSIP ++
Sbjct: 502 VYGRKYFGENFDRLVKIKTAVDPGNFFRNEQSIPTLK 537

BLAST of Bhi03G000165 vs. Swiss-Prot
Match: sp|Q9SA86|BBE9_ARATH (Berberine bridge enzyme-like 9 OS=Arabidopsis thaliana OX=3702 GN=At1g30710 PE=2 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 1.3e-155
Identity = 277/527 (52.56%), Postives = 370/527 (70.21%), Query Frame = 0

Query: 30  VLTTFLLILPSMAVSDQIPS-HQTLLKCLSDHSAPS-SSPISEVIFFPDNPSYSPVLNSY 89
           ++T FLL +P+   S   PS      +C++D    +  SPI   I+   +P++  +LN+Y
Sbjct: 10  IITIFLLTIPTSFASP--PSLEDVFAQCVTDFKPSNPKSPIQNYIYTQRSPNFLTILNNY 69

Query: 90  IRNLRFASPTTPKPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGLSYVSDSTF 149
           +RNLR+ +  T KP+ IVA   V+HIQATI C +   L++RIRSGGHDYDG+SY+S   F
Sbjct: 70  VRNLRYFNNMTRKPVAIVAAADVTHIQATITCAKKLGLQLRIRSGGHDYDGMSYLSTIDF 129

Query: 150 VILDMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKSK-IHGFPAGVCPTVGVGGHLS 209
           V+LDMFNLRS+ +D + ++AWV SGATLGE+Y+ +A KS  + GFPAG+CP +G GGH S
Sbjct: 130 VVLDMFNLRSINIDPKLDTAWVQSGATLGEIYYGVANKSNDLRGFPAGICPGLGAGGHFS 189

Query: 210 GAGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRASMGEDLFWAIRGGGGASFGVIVSWKF 269
           G GYGN+MRK+G+S+DN++DA IVD  GRVLDR+SMGEDLFWA+RGGG ASF V+++WK 
Sbjct: 190 GGGYGNMMRKYGLSIDNIIDAKIVDAKGRVLDRSSMGEDLFWALRGGGAASFCVVLAWKI 249

Query: 270 KLVPLPETVTVFRTEKTMEEGVV---DILYKWQEIADKIDENLFIRVVILPVNKKALKTA 329
           KLVP+P  VTVF  E     G V   +++ KWQEIADKID +LFIR+ +   N    KT 
Sbjct: 250 KLVPVPAKVTVFNIETFGNTGSVNTTELVAKWQEIADKIDNDLFIRLTLGSSN----KTV 309

Query: 330 KAKFISLFLGNAQKLFALMSKKLPELGIKVEDCIEMSWIDSVLFWSNYPIGT-PLNVLLE 389
           KA F+ ++LGN+  L  +M+ K PELG+   +CIEM WI+SVLFW   P GT P   +L 
Sbjct: 310 KASFMGMYLGNSSNLLEIMNAKFPELGLIKRECIEMKWIESVLFWLGIPPGTAPTTSMLN 369

Query: 390 RQPKSEKFLKKKSDYVQEPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFP 449
           R P+ + +LK+KSDYVQ+PIS+  LE + + M E +   + FNPYGG+MS+IP TET FP
Sbjct: 370 RIPQKQIYLKRKSDYVQKPISRTGLESIFKIMTENENVTMAFNPYGGRMSEIPSTETAFP 429

Query: 450 HRAGNKYKIQYSVTWKEEGDEAADKNVELIREMYEYMTPYVSKSPRSAYLNYRDVDLGVN 509
           HRAGN +KIQY+  W   G+  A   +     ++E M+PYVSK+PR A+LNYRDVD+G +
Sbjct: 430 HRAGNMFKIQYAANWFVPGEAVAKDCLSQTERLFEAMSPYVSKNPREAFLNYRDVDIGKS 489

Query: 510 GNG---HASSWGRKYFKGNFDRLVKVKSMVDPDNFFRYEQSIPCVEA 547
            N        +G KYFK NF++LVK+KS VDPDNFFRYEQSIP + +
Sbjct: 490 LNSTYEEGKVYGFKYFKDNFEKLVKIKSRVDPDNFFRYEQSIPVLSS 530

BLAST of Bhi03G000165 vs. TAIR10
Match: AT4G20840.1 (FAD-binding Berberine family protein)

HSP 1 Score: 568.5 bits (1464), Expect = 4.4e-162
Identity = 282/521 (54.13%), Postives = 370/521 (71.02%), Query Frame = 0

Query: 34  FLLILP---SMAVSDQIPSHQTLLKCLSDHSAPSSSPISEVIFFPDNPSYSPVLNSYIRN 93
           FL+ LP   S A       +++ ++C SD +    + I++ +F   NPS+S VL +YIRN
Sbjct: 16  FLVSLPFFSSAAPPSSDSIYESFVQCFSDKTKSPQAQITDNVFSRTNPSFSSVLRAYIRN 75

Query: 94  LRFASPTTPKPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGLSYVSDSTFVIL 153
            RF + +TPKP  IV P    H+ A + C +     ++IRSGGHDY+GLSY+SD  F IL
Sbjct: 76  GRFNTSSTPKPAIIVTPRSDIHVSAAVTCSKSLNFLLKIRSGGHDYEGLSYISDKPFFIL 135

Query: 154 DMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTVGVGGHLSGAGY 213
           DM NLR V+VDI D+SAW+ +GATLGEVY++I EKSK+HGFPAGVCPTVGVGGH+SG GY
Sbjct: 136 DMSNLRDVSVDIADQSAWISAGATLGEVYYRIWEKSKVHGFPAGVCPTVGVGGHISGGGY 195

Query: 214 GNLMRKFGVSVDNVVDALIVDVNGRVLDRASMGEDLFWAIRGGGGASFGVIVSWKFKLVP 273
           GN++RKFG+SVDN++DA IVDVNG++LDR SMGEDLFWAI GGGGASFGV++ +K KLVP
Sbjct: 196 GNMLRKFGLSVDNLIDAKIVDVNGQILDRKSMGEDLFWAISGGGGASFGVVLGYKVKLVP 255

Query: 274 LPETVTVFRTEKTMEEGVVDILYKWQEIADKIDENLFIRVVILPVNKKALKTAKAKFISL 333
           +PETVTVFR EK M+ G VD+++KWQ +  K D NLF+R++I PV +K +KT +A  ++L
Sbjct: 256 VPETVTVFRVEKYMDSGAVDMVHKWQSVGPKTDRNLFLRMLIQPVTRKKVKTVRATVVAL 315

Query: 334 FLGNAQKLFALMSKKLPELGIKVEDCIEMSWIDSVLFWSNYPIGTPLN--VLLERQPKSE 393
           FLG A+++ AL+ K+ PEL +K E+C EM+W  S L+W N    T ++  V L+R     
Sbjct: 316 FLGRAEEVVALLGKEFPELSLKKENCSEMTWFQSALWWDNRVNPTQIDPKVFLDRNLDRA 375

Query: 394 KFLKKKSDYVQEPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPHRAGNK 453
            F K+KSDYV   I +  +E + +KM EL +  L FNPYGGKM+++    TPFPHR+   
Sbjct: 376 NFGKRKSDYVASEIPRDGIESLFKKMTELGKIGLVFNPYGGKMAEVTVNATPFPHRS-KL 435

Query: 454 YKIQYSVTWKEEGDEAADKNVELIREMYEYMTPYVSKSPRSAYLNYRDVDLGVNGNG--- 513
           +KIQYSVTW+E   E     +     +Y +MT +VSK+PR+AYLNYRDVD+GVN +G   
Sbjct: 436 FKIQYSVTWQENSVEIEKGFLNQANVLYSFMTGFVSKNPRNAYLNYRDVDIGVNDHGTNS 495

Query: 514 --HASSWGRKYFKGNFDRLVKVKSMVDPDNFFRYEQSIPCV 545
                 +GRKYF  NFDRLVKVK+  DPDNFFR EQSIP V
Sbjct: 496 YEEGEVYGRKYFGDNFDRLVKVKTAADPDNFFRNEQSIPTV 535

BLAST of Bhi03G000165 vs. TAIR10
Match: AT1G34575.1 (FAD-binding Berberine family protein)

HSP 1 Score: 563.9 bits (1452), Expect = 1.1e-160
Identity = 281/521 (53.93%), Postives = 375/521 (71.98%), Query Frame = 0

Query: 30  VLTTFLLILP-SMAVSDQIPSHQTLLKCLSDHSAPSSSPISEVIFFPDNPSYSPVLNSYI 89
           + T FLL++P S A   ++    T  +C++      S PI    +   NP++  +LN+Y+
Sbjct: 10  IFTVFLLLIPTSFAAPPKLKDSFT--QCVTVFK--PSVPIQNFTYTQQNPNFLTILNNYV 69

Query: 90  RNLRFASPTTPKPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGLSYVSDSTFV 149
           RNLR+ + TT KP+ IVA  H +HIQATI C +   L++RIRSGGHDYDG+SY+S   FV
Sbjct: 70  RNLRYFNGTTRKPVAIVAAAHFTHIQATINCAKKLGLQLRIRSGGHDYDGMSYLSTVDFV 129

Query: 150 ILDMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKS-KIHGFPAGVCPTVGVGGHLSG 209
           +LDMFNLR++ +D + ++AWV SGATLGE+Y+ +A KS  + GFPAG+CP +G GGH SG
Sbjct: 130 VLDMFNLRAIEIDPKLDTAWVQSGATLGEIYYNVANKSNNLRGFPAGICPGLGAGGHFSG 189

Query: 210 AGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRASMGEDLFWAIRGGGGASFGVIVSWKFK 269
            GYGN+MRK+G+S+DN++DA IVD N RVLDR+SMGEDLFWA+RGGG ASF V+++WK K
Sbjct: 190 GGYGNMMRKYGLSIDNIIDAKIVDANARVLDRSSMGEDLFWALRGGGAASFCVVLAWKIK 249

Query: 270 LVPLPETVTVFRTEKTMEEGVV--DILYKWQEIADKIDENLFIRVVILPVNKKALKTAKA 329
           LVP+PE VTVF  E     GV+  D+  KWQEIADKID +LFIR+ +   N    KT KA
Sbjct: 250 LVPVPEKVTVFNVETIGNRGVIPTDLAAKWQEIADKIDNDLFIRLTLSSSN----KTVKA 309

Query: 330 KFISLFLGNAQKLFALMSKKLPELGIKVEDCIEMSWIDSVLFWSNYPIGT-PLNVLLERQ 389
            F+ ++LGN++KL  +M+ K PELG+   +CIEM WI+SVLFW + P GT P +V+L R 
Sbjct: 310 SFMGMYLGNSEKLLEIMNAKFPELGLNKTECIEMKWIESVLFWLSIPPGTAPTSVMLNRI 369

Query: 390 PKSEKFLKKKSDYVQEPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPHR 449
           P+ + +LK+KSDYVQ+PISK  LE + + + E +  ++ +NPYGG+MS+IP TET FPHR
Sbjct: 370 PQKQIYLKRKSDYVQKPISKPGLESIFKILSENENVSMAWNPYGGRMSEIPATETAFPHR 429

Query: 450 AGNKYKIQYSVTWKEEGDEAADKNVELIREMYEYMTPYVSKSPRSAYLNYRDVDLGVNGN 509
           AGN +KIQYS  W   G+EAA   +     ++E M+PYVSK+PR A+LNYRD+D+G N N
Sbjct: 430 AGNMFKIQYSSNWFVPGEEAASDCLSQTERVFEAMSPYVSKNPREAFLNYRDIDIGKNLN 489

Query: 510 G---HASSWGRKYFKGNFDRLVKVKSMVDPDNFFRYEQSIP 543
                   +G KYFK NF+RLV+VK+ VDPDN FRYEQSIP
Sbjct: 490 STYEEGKVYGVKYFKNNFERLVQVKTRVDPDNIFRYEQSIP 522

BLAST of Bhi03G000165 vs. TAIR10
Match: AT1G30700.1 (FAD-binding Berberine family protein)

HSP 1 Score: 563.5 bits (1451), Expect = 1.4e-160
Identity = 280/524 (53.44%), Postives = 379/524 (72.33%), Query Frame = 0

Query: 30  VLTTFLLILPSMAVSDQIPSHQTLLKCLSDHSAPSSSPISEVIFFPDNPSYSPVLNSYIR 89
           +L  F ++    + S    S +T  +CL+ +S P   PIS  IFF  N SYS VL + IR
Sbjct: 6   ILVLFFVVFIWQSSSSSANS-ETFTQCLTSNSDP-KHPISPAIFFSGNGSYSSVLQANIR 65

Query: 90  NLRFASPTTPKPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGLSYV--SDSTF 149
           NLRF + +TPKP  I+A TH SH+QA I C + H L+++IRSGGHDYDGLSYV  S   F
Sbjct: 66  NLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPF 125

Query: 150 VILDMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTVGVGGHLSG 209
            +LDMFNLRSV VD+  ++AWV +GA LGEVY+ I EKSK   +PAG+CPTVGVGGH+SG
Sbjct: 126 FVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISG 185

Query: 210 AGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRASMGEDLFWAIRGGGGASFGVIVSWKFK 269
            GYGN+MRK+G++VDN +DA +VDVNG++LDR  MGEDL+WAI GGGG S+GV++++K  
Sbjct: 186 GGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKIN 245

Query: 270 LVPLPETVTVFRTEKTMEEGVVDILYKWQEIADKIDENLFIRVVILPVN--KKALKTAKA 329
           LV +PE VTVFR  +T+E+   DI+++WQ++A K+ + LFIR VI  VN    + KT + 
Sbjct: 246 LVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTVRT 305

Query: 330 KFISLFLGNAQKLFALMSKKLPELGIKVEDCIEMSWIDSVLFWSNYPIGTPLNVLLERQP 389
            FI++FLG+   L ++++++ PELG+   DC E SWI SVLFW+N  +G+   +LL+R  
Sbjct: 306 TFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSSETLLLQRN- 365

Query: 390 KSEKFLKKKSDYVQEPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPHRA 449
           +   +LK+KSDYV+EPIS+  LE + +KMIEL+ P + FNPYGG+M +I  T TPFP+RA
Sbjct: 366 QPVNYLKRKSDYVREPISRTGLESIWKKMIELEIPTMAFNPYGGEMGRISSTVTPFPYRA 425

Query: 450 GNKYKIQYSVTWKEEGDEAADKNVELIREMYEYMTPYVSKSPRSAYLNYRDVDLGVNG-N 509
           GN +KIQY   W++E     D+ +EL R++Y++MTP+VSK+PR ++ NYRDVDLG+N  N
Sbjct: 426 GNLWKIQYGANWRDE--TLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGINSHN 485

Query: 510 GHASS------WGRKYFKGNFDRLVKVKSMVDPDNFFRYEQSIP 543
           G  SS      +G+KYF GNF+RLVK+K+ VD  NFFR EQSIP
Sbjct: 486 GKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIP 524

BLAST of Bhi03G000165 vs. TAIR10
Match: AT4G20830.1 (FAD-binding Berberine family protein)

HSP 1 Score: 559.3 bits (1440), Expect = 2.7e-159
Identity = 276/517 (53.38%), Postives = 361/517 (69.83%), Query Frame = 0

Query: 36  LILPSMAVSDQIPSHQTLLKCLSDHSAPSSSPISEVIFFPDNPSYSPVLNSYIRNLRFAS 95
           L LP  + S     + + LKC SD +    S I++ +F   NP++S VL +YIRN RF +
Sbjct: 22  LSLPLSSFSQSNSVYNSFLKCFSDKTKSPQSQITDNVFSQTNPAFSSVLRAYIRNARFNT 81

Query: 96  PTTPKPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGLSYVSDSTFVILDMFNL 155
            +T KP  I+ P   SH+ A + C +     ++IRSGGHDYDGLSY+SD  F ILDM N+
Sbjct: 82  SSTLKPTIIITPRSESHVSAAVTCSKTLNFLLKIRSGGHDYDGLSYISDKPFFILDMSNI 141

Query: 156 RSVAVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTVGVGGHLSGAGYGNLMR 215
           R V+VDI   SAW+ +GATLGEVY++I EKS++HGFPAGVCPTVGVGGHLSG GYGN++R
Sbjct: 142 RDVSVDIASNSAWISAGATLGEVYYRIWEKSRVHGFPAGVCPTVGVGGHLSGGGYGNMVR 201

Query: 216 KFGVSVDNVVDALIVDVNGRVLDRASMGEDLFWAIRGGGGASFGVIVSWKFKLVPLPETV 275
           KFG+SVD V DA IVDVNGRVLDR +MGEDLFWAI GGGG S+GV++ +K KLVP+P  V
Sbjct: 202 KFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGGGGSYGVVLGYKVKLVPVPSVV 261

Query: 276 TVFRTEKTMEEGVVDILYKWQEIADKIDENLFIRVVILPVNKKALKTAKAKFISLFLGNA 335
           TVFR E+ M+ G VD+++KWQ +  K D NLF+R++I PV +K +KT +A  ++LFLG A
Sbjct: 262 TVFRVEQYMDSGAVDMVHKWQSVGPKTDPNLFMRMLIQPVTRKKVKTVRASVVALFLGRA 321

Query: 336 QKLFALMSKKLPELGIKVEDCIEMSWIDSVLFWSNYPIGTPLN--VLLERQPKSEKFLKK 395
            ++ AL+SK+ PELG+K E+C EM+W  S L+W N    T ++  V L+R   +  F K+
Sbjct: 322 DEVVALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDPKVFLDRNLDTSSFGKR 381

Query: 396 KSDYVQEPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPHRAGNKYKIQY 455
           KSDYV   I K  +E + +KMIEL +  L FNPYGGKM+++     PFPHR    +KIQY
Sbjct: 382 KSDYVATAIPKKGIESLFKKMIELGKIGLVFNPYGGKMAEVAVNAKPFPHR-NKLFKIQY 441

Query: 456 SVTWKEEGDEAADKNVELIREMYEYMTPYVSKSPRSAYLNYRDVDLGVNGNG-----HAS 515
           SV WKE   E     +   + +Y +MT +VSK+PRS+Y NYRDVD+GVN +G        
Sbjct: 442 SVNWKENSAEIEKGYLNQAKVLYSFMTGFVSKNPRSSYFNYRDVDIGVNDHGANSYKEGE 501

Query: 516 SWGRKYFKGNFDRLVKVKSMVDPDNFFRYEQSIPCVE 546
            +GRKYF  NFDRLVK+K+ VDP NFFR EQSIP ++
Sbjct: 502 VYGRKYFGENFDRLVKIKTAVDPGNFFRNEQSIPTLK 537

BLAST of Bhi03G000165 vs. TAIR10
Match: AT1G30710.1 (FAD-binding Berberine family protein)

HSP 1 Score: 551.2 bits (1419), Expect = 7.3e-157
Identity = 277/527 (52.56%), Postives = 370/527 (70.21%), Query Frame = 0

Query: 30  VLTTFLLILPSMAVSDQIPS-HQTLLKCLSDHSAPS-SSPISEVIFFPDNPSYSPVLNSY 89
           ++T FLL +P+   S   PS      +C++D    +  SPI   I+   +P++  +LN+Y
Sbjct: 10  IITIFLLTIPTSFASP--PSLEDVFAQCVTDFKPSNPKSPIQNYIYTQRSPNFLTILNNY 69

Query: 90  IRNLRFASPTTPKPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGLSYVSDSTF 149
           +RNLR+ +  T KP+ IVA   V+HIQATI C +   L++RIRSGGHDYDG+SY+S   F
Sbjct: 70  VRNLRYFNNMTRKPVAIVAAADVTHIQATITCAKKLGLQLRIRSGGHDYDGMSYLSTIDF 129

Query: 150 VILDMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKSK-IHGFPAGVCPTVGVGGHLS 209
           V+LDMFNLRS+ +D + ++AWV SGATLGE+Y+ +A KS  + GFPAG+CP +G GGH S
Sbjct: 130 VVLDMFNLRSINIDPKLDTAWVQSGATLGEIYYGVANKSNDLRGFPAGICPGLGAGGHFS 189

Query: 210 GAGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRASMGEDLFWAIRGGGGASFGVIVSWKF 269
           G GYGN+MRK+G+S+DN++DA IVD  GRVLDR+SMGEDLFWA+RGGG ASF V+++WK 
Sbjct: 190 GGGYGNMMRKYGLSIDNIIDAKIVDAKGRVLDRSSMGEDLFWALRGGGAASFCVVLAWKI 249

Query: 270 KLVPLPETVTVFRTEKTMEEGVV---DILYKWQEIADKIDENLFIRVVILPVNKKALKTA 329
           KLVP+P  VTVF  E     G V   +++ KWQEIADKID +LFIR+ +   N    KT 
Sbjct: 250 KLVPVPAKVTVFNIETFGNTGSVNTTELVAKWQEIADKIDNDLFIRLTLGSSN----KTV 309

Query: 330 KAKFISLFLGNAQKLFALMSKKLPELGIKVEDCIEMSWIDSVLFWSNYPIGT-PLNVLLE 389
           KA F+ ++LGN+  L  +M+ K PELG+   +CIEM WI+SVLFW   P GT P   +L 
Sbjct: 310 KASFMGMYLGNSSNLLEIMNAKFPELGLIKRECIEMKWIESVLFWLGIPPGTAPTTSMLN 369

Query: 390 RQPKSEKFLKKKSDYVQEPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFP 449
           R P+ + +LK+KSDYVQ+PIS+  LE + + M E +   + FNPYGG+MS+IP TET FP
Sbjct: 370 RIPQKQIYLKRKSDYVQKPISRTGLESIFKIMTENENVTMAFNPYGGRMSEIPSTETAFP 429

Query: 450 HRAGNKYKIQYSVTWKEEGDEAADKNVELIREMYEYMTPYVSKSPRSAYLNYRDVDLGVN 509
           HRAGN +KIQY+  W   G+  A   +     ++E M+PYVSK+PR A+LNYRDVD+G +
Sbjct: 430 HRAGNMFKIQYAANWFVPGEAVAKDCLSQTERLFEAMSPYVSKNPREAFLNYRDVDIGKS 489

Query: 510 GNG---HASSWGRKYFKGNFDRLVKVKSMVDPDNFFRYEQSIPCVEA 547
            N        +G KYFK NF++LVK+KS VDPDNFFRYEQSIP + +
Sbjct: 490 LNSTYEEGKVYGFKYFKDNFEKLVKIKSRVDPDNFFRYEQSIPVLSS 530

BLAST of Bhi03G000165 vs. TrEMBL
Match: tr|A0A1S3AZM6|A0A1S3AZM6_CUCME (reticuline oxidase-like protein OS=Cucumis melo OX=3656 GN=LOC103484295 PE=3 SV=1)

HSP 1 Score: 1006.5 bits (2601), Expect = 2.3e-290
Identity = 508/566 (89.75%), Postives = 531/566 (93.82%), Query Frame = 0

Query: 1   MEMPGCFFCFRHLPPNAISMG--IPNSLLIPVLTTFLLILPSMAVSDQIPSHQTLLKCLS 60
           M++P CFF F HLPP AISMG  IP+S+LIP LTT LLIL SMA+S Q PSHQTLL CLS
Sbjct: 1   MQIPCCFFSFHHLPPKAISMGIPIPDSVLIPTLTTLLLILSSMALSAQTPSHQTLLDCLS 60

Query: 61  DHS-APSSSPISEVIFFPDNPSYSPVLNSYIRNLRFASPTTPKPLFIVAPTHVSHIQATI 120
           DHS +PSSSPIS+V FFPDNPSYSPVLNSYIRNLRFASPTTPKPLFIVAPTHVSHIQATI
Sbjct: 61  DHSNSPSSSPISQVTFFPDNPSYSPVLNSYIRNLRFASPTTPKPLFIVAPTHVSHIQATI 120

Query: 121 LCCRIHALEIRIRSGGHDYDGLSYVSDSTFVILDMFNLRSVAVDIEDESAWVDSGATLGE 180
           LCCRIHALEIRIRSGGHDYDGLSYVSDS FVILDMFNLRSVAVDIEDESAWVDSGATLGE
Sbjct: 121 LCCRIHALEIRIRSGGHDYDGLSYVSDSPFVILDMFNLRSVAVDIEDESAWVDSGATLGE 180

Query: 181 VYFKIAEKSKIHGFPAGVCPTVGVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDVNGRVL 240
           VY+KIAEKSKIHGFPAGVCPTVGVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDVNGRVL
Sbjct: 181 VYYKIAEKSKIHGFPAGVCPTVGVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDVNGRVL 240

Query: 241 DRASMGEDLFWAIRGGGGASFGVIVSWKFKLVPLPETVTVFRTEKTMEEGVVDILYKWQE 300
           DR +MGEDLFWAIRGGGGASFGVIVSWKFKLV LPETVTVFRTEKT+EEGVVDIL+KWQE
Sbjct: 241 DRETMGEDLFWAIRGGGGASFGVIVSWKFKLVLLPETVTVFRTEKTIEEGVVDILHKWQE 300

Query: 301 IADKIDENLFIRVVILPVNKKALKTAKAKFISLFLGNAQKLFALMSKKLPELGIKVEDCI 360
           IAD IDENLFIRVVILPVNKK  KTAKAKF+SLFLGNAQKLFALMS++ PELGIK EDC 
Sbjct: 301 IADTIDENLFIRVVILPVNKKTQKTAKAKFVSLFLGNAQKLFALMSERFPELGIKGEDCK 360

Query: 361 EMSWIDSVLFWSNYPIGTPLNVLLERQPKSEKFLKKKSDYVQEPISKADLEGMMRKMIEL 420
           EMSWIDS+LFWSNYPIGTPL+VLLERQP SEKFLKKKSDYVQEPISKA LEGMMRKMIEL
Sbjct: 361 EMSWIDSILFWSNYPIGTPLDVLLERQPNSEKFLKKKSDYVQEPISKAGLEGMMRKMIEL 420

Query: 421 KRPALTFNPYGGKMSQIPETETPFPHRAGNKYKIQYSVTWKEEGDEAADKNVELIREMYE 480
           KRPALTFNPYGGKMSQIPETETPFPHRAGNKYKIQYSVTWKEEGDEAA K++E+IRE+Y+
Sbjct: 421 KRPALTFNPYGGKMSQIPETETPFPHRAGNKYKIQYSVTWKEEGDEAAAKHLEMIRELYK 480

Query: 481 YMTPYVSKSPRSAYLNYRDVDLGVNGNGHA-----SSWGRKYFKGNFDRLVKVKSMVDPD 540
           YMTPYVSKSPRSAYLNYRDVDLGVNG G+A     S WGRKYFKGNFDRLVKVKSMVDPD
Sbjct: 481 YMTPYVSKSPRSAYLNYRDVDLGVNGIGNASYWEGSIWGRKYFKGNFDRLVKVKSMVDPD 540

Query: 541 NFFRYEQSIPCV--EAFDSNGRAESL 557
           NFF+YEQSIPCV  EAFDSNGRAESL
Sbjct: 541 NFFKYEQSIPCVELEAFDSNGRAESL 566

BLAST of Bhi03G000165 vs. TrEMBL
Match: tr|A0A2P6Q6J5|A0A2P6Q6J5_ROSCH (Putative tetrahydroberberine oxidase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr5g0017721 PE=3 SV=1)

HSP 1 Score: 771.5 bits (1991), Expect = 1.2e-219
Identity = 371/540 (68.70%), Postives = 449/540 (83.15%), Query Frame = 0

Query: 14  PPNAISMGIPNSLLIPVLTTFLLILPSMAVSDQIPSHQTLLKCLSDHSAPSSSPISEVIF 73
           P + I M    +     + + LL+  SMA SD  P   T L+CLS+ S   S PIS V +
Sbjct: 8   PSSQIVMKTQTTSATLAIVSSLLLHASMAASD--PIQDTFLQCLSNLSQSPSPPISSVTY 67

Query: 74  FPDNPSYSPVLNSYIRNLRFASPTTPKPLFIVAPTHVSHIQATILCCRIHALEIRIRSGG 133
           FP NPSY+P+L+SYIRNLRF +P TPKP FIV PTHVSHIQA+ILC +IH L++RIRSGG
Sbjct: 68  FPTNPSYTPILHSYIRNLRFTTPKTPKPSFIVTPTHVSHIQASILCSKIHNLQVRIRSGG 127

Query: 134 HDYDGLSYVSDSTFVILDMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPA 193
           HDYDGLSY+SD  F+I+DMFNLRS+ V++EDESAWV+SGATLGEVY++IAE+SKIHG+PA
Sbjct: 128 HDYDGLSYISDVPFIIIDMFNLRSITVNVEDESAWVESGATLGEVYYRIAEQSKIHGYPA 187

Query: 194 GVCPTVGVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRASMGEDLFWAIRGG 253
           GVCPTVGVGGHLSG GYGN+MRK G+SVDN++DA IVDVNG VLDR SMGEDLFWAIRGG
Sbjct: 188 GVCPTVGVGGHLSGGGYGNMMRKHGLSVDNILDAQIVDVNGSVLDRESMGEDLFWAIRGG 247

Query: 254 GGASFGVIVSWKFKLVPLPETVTVFRTEKTMEEGVVDILYKWQEIADKIDENLFIRVVIL 313
           GGASFGVIVSWK KLV +PE VTVFR E+T+E+G  DI+++WQ + D ID++LFIRVV++
Sbjct: 248 GGASFGVIVSWKIKLVQVPEVVTVFRVERTLEQGATDIVHQWQYVGDTIDDDLFIRVVVM 307

Query: 314 PVNKKALKTAKAKFISLFLGNAQKLFALMSKKLPELGIKVEDCIEMSWIDSVLFWSNYPI 373
           PVN+K  KT KAKF++L+LGNA+KL ALM ++ P+LG+K ED +E  WI+SVL+WSNYPI
Sbjct: 308 PVNRKTQKTVKAKFVALYLGNAEKLEALMGERFPQLGLKHEDYVERGWIESVLYWSNYPI 367

Query: 374 GTPLNVLLERQPKSEKFLKKKSDYVQEPISKADLEGMMRKMIELKRPALTFNPYGGKMSQ 433
           GT  ++LLER P+SEKFLKKKSDY+QEP+SKA LEG+  KM+ELK+P LTFNPYGGKMS+
Sbjct: 368 GTSADILLERIPQSEKFLKKKSDYIQEPMSKAGLEGLWNKMMELKKPVLTFNPYGGKMSE 427

Query: 434 IPETETPFPHRAGNKYKIQYSVTWKEEGDEAADKNVELIREMYEYMTPYVSKSPRSAYLN 493
           IPE +TPFPHRAGN YKIQYSV WKEEG EA DK+++LIR +YE+MTPYVS SPR +YLN
Sbjct: 428 IPELDTPFPHRAGNVYKIQYSVNWKEEGVEAEDKHLDLIRRLYEHMTPYVSTSPRCSYLN 487

Query: 494 YRDVDLGVNGNGH-----ASSWGRKYFKGNFDRLVKVKSMVDPDNFFRYEQSIPCVEAFD 549
           YRDVDLG NGNG+     AS WG KYFKGNF+RLV+VK+ VDP NFFRYEQSIP + A++
Sbjct: 488 YRDVDLGTNGNGNVSYSEASVWGTKYFKGNFERLVQVKTAVDPGNFFRYEQSIPPLTAWN 545

BLAST of Bhi03G000165 vs. TrEMBL
Match: tr|A0A2P5ARH5|A0A2P5ARH5_PARAD (Xanthine dehydrogenase C subunit OS=Parasponia andersonii OX=3476 GN=PanWU01x14_307130 PE=3 SV=1)

HSP 1 Score: 770.8 bits (1989), Expect = 2.1e-219
Identity = 368/513 (71.73%), Postives = 441/513 (85.96%), Query Frame = 0

Query: 41  MAVSDQIPSHQTLLKCLSDHSAPSSSPISEVIFFPDNPSYSPVLNSYIRNLRFASPTTPK 100
           MA SDQ  S Q+ L+CLS HS  S  PIS V FFP++PSY  VLNSYIRNLRF S TTPK
Sbjct: 1   MAFSDQ--SQQSFLQCLSTHSQTSPIPISSVTFFPNDPSYPSVLNSYIRNLRFISSTTPK 60

Query: 101 PLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGLSYVSDSTFVILDMFNLRSVAV 160
           PLFI+APTHVSHIQA+I+CC+IH L++RIRSGGHDYDGLSYVSD  F+++DMFNLR+++V
Sbjct: 61  PLFIIAPTHVSHIQASIICCKIHGLQVRIRSGGHDYDGLSYVSDDPFIVVDMFNLRNISV 120

Query: 161 DIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTVGVGGHLSGAGYGNLMRKFGVS 220
           +IEDE+AWV+SGAT+GE+Y+KIAE+S+IHGFPAGVCPTVGVGGH SG GYGN+MRK G+S
Sbjct: 121 NIEDETAWVESGATIGELYYKIAEQSRIHGFPAGVCPTVGVGGHFSGGGYGNMMRKHGLS 180

Query: 221 VDNVVDALIVDVNGRVLDRASMGEDLFWAIRGGGGASFGVIVSWKFKLVPLPETVTVFRT 280
           VDNV+DAL+VDVNGRVL R SMGEDLFWAIRGGGGASFGVIVSWK KLV +PE VTVFR 
Sbjct: 181 VDNVIDALLVDVNGRVLSRTSMGEDLFWAIRGGGGASFGVIVSWKIKLVSVPENVTVFRV 240

Query: 281 EKTMEEGVVDILYKWQEIADKIDENLFIRVVILPVNKKALKTAKAKFISLFLGNAQKLFA 340
           E+T+E+G  DI+ +WQ++ADK+ + +FIRVV++PVNKK  KT KAKF++L+LG A+++ A
Sbjct: 241 ERTLEQGATDIVLQWQDVADKMLDEMFIRVVLMPVNKKIQKTVKAKFVALYLGPAEEVTA 300

Query: 341 LMSKKLPELGIKVEDCIEMSWIDSVLFWSNYPIGTPLNVLLERQPKSEKFLKKKSDYVQE 400
           LM+++ P+LG+  ED IEMSWI+SVLFWSNYPIGT ++VLLERQPKSEKFLKKKSDYVQE
Sbjct: 301 LMAERFPKLGLTREDYIEMSWIESVLFWSNYPIGTSMDVLLERQPKSEKFLKKKSDYVQE 360

Query: 401 PISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPHRAGNKYKIQYSVTWKEE 460
           PI K++LEG+ +KMIELK+PAL FNPYGGKMS+I E ETPFPHRAGNKYKIQYSV WKEE
Sbjct: 361 PIKKSELEGLWKKMIELKKPALAFNPYGGKMSEISELETPFPHRAGNKYKIQYSVQWKEE 420

Query: 461 GDEAADKNVELIREMYEYMTPYVSKSPRSAYLNYRDVDLGVNGNGH------ASSWGRKY 520
                D+N++ IR++YEYMTP VSKSPR +YLNYRDVDLG NG G+      AS WG KY
Sbjct: 421 DTRTLDQNLDRIRKLYEYMTPLVSKSPRCSYLNYRDVDLGTNGVGYNGTYREASIWGTKY 480

Query: 521 FKGNFDRLVKVKSMVDPDNFFRYEQSIPCVEAF 548
           FKGN+ RLV+VK+ VDP NFFRYEQSIP + A+
Sbjct: 481 FKGNYGRLVQVKTEVDPGNFFRYEQSIPPLAAW 511

BLAST of Bhi03G000165 vs. TrEMBL
Match: tr|A0A2P5FTI6|A0A2P5FTI6_9ROSA (Xanthine dehydrogenase C subunit OS=Trema orientalis OX=63057 GN=TorRG33x02_032700 PE=3 SV=1)

HSP 1 Score: 768.1 bits (1982), Expect = 1.4e-218
Identity = 367/513 (71.54%), Postives = 440/513 (85.77%), Query Frame = 0

Query: 41  MAVSDQIPSHQTLLKCLSDHSAPSSSPISEVIFFPDNPSYSPVLNSYIRNLRFASPTTPK 100
           MA SDQ  S Q+ L+CLS HS  S  PIS V FFP++PSY  VLNSYIRNLRF S TTPK
Sbjct: 1   MAFSDQ--SQQSFLRCLSTHSQTSPIPISSVTFFPNDPSYPLVLNSYIRNLRFISSTTPK 60

Query: 101 PLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGLSYVSDSTFVILDMFNLRSVAV 160
           PLFI+APTHVSHIQA+I+CC+IH L++RIRSGGHDYDGLSYVSD  F+++DMFNLR++ V
Sbjct: 61  PLFIIAPTHVSHIQASIICCKIHGLQVRIRSGGHDYDGLSYVSDDPFIVVDMFNLRNITV 120

Query: 161 DIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTVGVGGHLSGAGYGNLMRKFGVS 220
           +IEDE+AWV+SGAT+GE+Y+KIAE+S+IHGFPAGVCPTVGVGGH SG GYGN+MRK G+S
Sbjct: 121 NIEDEAAWVESGATIGELYYKIAEQSRIHGFPAGVCPTVGVGGHFSGGGYGNMMRKHGLS 180

Query: 221 VDNVVDALIVDVNGRVLDRASMGEDLFWAIRGGGGASFGVIVSWKFKLVPLPETVTVFRT 280
           VDN++DALIVDVNGRVL R SMGEDLFWAIRGGGGASFGVIVSWK KLV +PE VTVFR 
Sbjct: 181 VDNILDALIVDVNGRVLSRTSMGEDLFWAIRGGGGASFGVIVSWKIKLVSVPENVTVFRV 240

Query: 281 EKTMEEGVVDILYKWQEIADKIDENLFIRVVILPVNKKALKTAKAKFISLFLGNAQKLFA 340
           E+T+E+G  DI+ +WQ++ADK+ + +FIRVV++PVNKK  KT KAKF++L+LG A+++ A
Sbjct: 241 ERTLEQGATDIVLQWQDVADKMLDEMFIRVVLMPVNKKNQKTVKAKFVALYLGPAEEVTA 300

Query: 341 LMSKKLPELGIKVEDCIEMSWIDSVLFWSNYPIGTPLNVLLERQPKSEKFLKKKSDYVQE 400
           LM+++ P+LG+  ED IEMSWI+SVLFWSNYPIGT ++VLLERQPKSEKFLKKKSDYVQE
Sbjct: 301 LMAERFPKLGLTREDYIEMSWIESVLFWSNYPIGTSIDVLLERQPKSEKFLKKKSDYVQE 360

Query: 401 PISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPHRAGNKYKIQYSVTWKEE 460
           PI K++LEG+ +KMIELK+PAL FNPYGGKMS+I + ETPFPHRAGNKYKIQYSV WKEE
Sbjct: 361 PIKKSELEGLWKKMIELKKPALAFNPYGGKMSEISQLETPFPHRAGNKYKIQYSVQWKEE 420

Query: 461 GDEAADKNVELIREMYEYMTPYVSKSPRSAYLNYRDVDLGVNGNGH------ASSWGRKY 520
                D+N++ IR++YEYMTP VSKSPR +YLNYRDVDLG NG G+      AS WG KY
Sbjct: 421 DTRTLDQNLDRIRKLYEYMTPLVSKSPRCSYLNYRDVDLGTNGVGYNGTYREASIWGTKY 480

Query: 521 FKGNFDRLVKVKSMVDPDNFFRYEQSIPCVEAF 548
           FKGN+ RLV+VK+ VDP NFFRYEQSIP + A+
Sbjct: 481 FKGNYGRLVQVKTEVDPGNFFRYEQSIPPLAAW 511

BLAST of Bhi03G000165 vs. TrEMBL
Match: tr|M5X1T9|M5X1T9_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_4G098400 PE=3 SV=1)

HSP 1 Score: 767.7 bits (1981), Expect = 1.8e-218
Identity = 369/514 (71.79%), Postives = 440/514 (85.60%), Query Frame = 0

Query: 41  MAVSDQIPSHQT-LLKCLSDHSAPSSSPISEVIFFPDNPSYSPVLNSYIRNLRFASPTTP 100
           MA S+  P+  T  L+CLS+HS  SS PIS V +FP+N SY+ +L SYIRNLRF +PTTP
Sbjct: 1   MAASN--PTQDTFFLQCLSNHSQYSSPPISAVTYFPNNSSYTSILQSYIRNLRFITPTTP 60

Query: 101 KPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGLSYVSDSTFVILDMFNLRSVA 160
           KPLFIV PTHVSHIQA+I C +IHAL++RIRSGGHDYDGLSYVS   F+I+DMF+LRS++
Sbjct: 61  KPLFIVTPTHVSHIQASIFCSKIHALQVRIRSGGHDYDGLSYVSQVPFIIVDMFSLRSIS 120

Query: 161 VDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTVGVGGHLSGAGYGNLMRKFGV 220
           V +EDESAWV+SGATLGEVY+++AE+SKIHGFPAGVCPTVGVGGHLSG GYGN+MRK+G+
Sbjct: 121 VKVEDESAWVESGATLGEVYYRVAEQSKIHGFPAGVCPTVGVGGHLSGGGYGNMMRKYGL 180

Query: 221 SVDNVVDALIVDVNGRVLDRASMGEDLFWAIRGGGGASFGVIVSWKFKLVPLPETVTVFR 280
           SVDN++DA IVDVNGR+LDR SMGEDLFWAIRGGGGASFGVIVSWK KLVP+PE VTVFR
Sbjct: 181 SVDNILDAQIVDVNGRLLDRESMGEDLFWAIRGGGGASFGVIVSWKIKLVPVPEIVTVFR 240

Query: 281 TEKTMEEGVVDILYKWQEIADKIDENLFIRVVILPVNKKALKTAKAKFISLFLGNAQKLF 340
            E+T+E+G  DI+++WQ +AD I E+LFIR V++  NKK  +T KAKF++LFLGNA+KL 
Sbjct: 241 VERTLEQGATDIVHQWQYVADTIHEDLFIRAVVMQSNKKGHQTVKAKFVALFLGNAKKLH 300

Query: 341 ALMSKKLPELGIKVEDCIEMSWIDSVLFWSNYPIGTPLNVLLERQPKSEKFLKKKSDYVQ 400
           ALM + LP+LG+K ED IEMSWI+SVL+WSNYPIGT  +VLLERQP SEKFLKKKSDY+Q
Sbjct: 301 ALMDESLPQLGLKHEDYIEMSWIESVLYWSNYPIGTSTDVLLERQPSSEKFLKKKSDYIQ 360

Query: 401 EPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPHRAGNKYKIQYSVTWKE 460
           EP+SK+ LE + +KM+EL++P LTFNPYGGKM +I E ETPFPHRAGN YKIQYSV WKE
Sbjct: 361 EPMSKSGLEELWKKMMELRKPVLTFNPYGGKMREISELETPFPHRAGNVYKIQYSVNWKE 420

Query: 461 EGDEAADKNVELIREMYEYMTPYVSKSPRSAYLNYRDVDLGVNGNGH-----ASSWGRKY 520
           EG EAADKN++LIR +YE+MTPYVSKSPR +YLNYRDVDLG NGNG+     AS WG KY
Sbjct: 421 EGVEAADKNLDLIRRLYEFMTPYVSKSPRCSYLNYRDVDLGTNGNGNASYSEASVWGTKY 480

Query: 521 FKGNFDRLVKVKSMVDPDNFFRYEQSIPCVEAFD 549
           FKGNFDRLV+VK+ VDP NFF YEQSIP +  ++
Sbjct: 481 FKGNFDRLVQVKTAVDPGNFFSYEQSIPSLTGWE 512

BLAST of Bhi03G000165 vs. NCBI nr
Match: XP_008439526.1 (PREDICTED: reticuline oxidase-like protein [Cucumis melo])

HSP 1 Score: 1006.5 bits (2601), Expect = 3.5e-290
Identity = 508/566 (89.75%), Postives = 531/566 (93.82%), Query Frame = 0

Query: 1   MEMPGCFFCFRHLPPNAISMG--IPNSLLIPVLTTFLLILPSMAVSDQIPSHQTLLKCLS 60
           M++P CFF F HLPP AISMG  IP+S+LIP LTT LLIL SMA+S Q PSHQTLL CLS
Sbjct: 1   MQIPCCFFSFHHLPPKAISMGIPIPDSVLIPTLTTLLLILSSMALSAQTPSHQTLLDCLS 60

Query: 61  DHS-APSSSPISEVIFFPDNPSYSPVLNSYIRNLRFASPTTPKPLFIVAPTHVSHIQATI 120
           DHS +PSSSPIS+V FFPDNPSYSPVLNSYIRNLRFASPTTPKPLFIVAPTHVSHIQATI
Sbjct: 61  DHSNSPSSSPISQVTFFPDNPSYSPVLNSYIRNLRFASPTTPKPLFIVAPTHVSHIQATI 120

Query: 121 LCCRIHALEIRIRSGGHDYDGLSYVSDSTFVILDMFNLRSVAVDIEDESAWVDSGATLGE 180
           LCCRIHALEIRIRSGGHDYDGLSYVSDS FVILDMFNLRSVAVDIEDESAWVDSGATLGE
Sbjct: 121 LCCRIHALEIRIRSGGHDYDGLSYVSDSPFVILDMFNLRSVAVDIEDESAWVDSGATLGE 180

Query: 181 VYFKIAEKSKIHGFPAGVCPTVGVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDVNGRVL 240
           VY+KIAEKSKIHGFPAGVCPTVGVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDVNGRVL
Sbjct: 181 VYYKIAEKSKIHGFPAGVCPTVGVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDVNGRVL 240

Query: 241 DRASMGEDLFWAIRGGGGASFGVIVSWKFKLVPLPETVTVFRTEKTMEEGVVDILYKWQE 300
           DR +MGEDLFWAIRGGGGASFGVIVSWKFKLV LPETVTVFRTEKT+EEGVVDIL+KWQE
Sbjct: 241 DRETMGEDLFWAIRGGGGASFGVIVSWKFKLVLLPETVTVFRTEKTIEEGVVDILHKWQE 300

Query: 301 IADKIDENLFIRVVILPVNKKALKTAKAKFISLFLGNAQKLFALMSKKLPELGIKVEDCI 360
           IAD IDENLFIRVVILPVNKK  KTAKAKF+SLFLGNAQKLFALMS++ PELGIK EDC 
Sbjct: 301 IADTIDENLFIRVVILPVNKKTQKTAKAKFVSLFLGNAQKLFALMSERFPELGIKGEDCK 360

Query: 361 EMSWIDSVLFWSNYPIGTPLNVLLERQPKSEKFLKKKSDYVQEPISKADLEGMMRKMIEL 420
           EMSWIDS+LFWSNYPIGTPL+VLLERQP SEKFLKKKSDYVQEPISKA LEGMMRKMIEL
Sbjct: 361 EMSWIDSILFWSNYPIGTPLDVLLERQPNSEKFLKKKSDYVQEPISKAGLEGMMRKMIEL 420

Query: 421 KRPALTFNPYGGKMSQIPETETPFPHRAGNKYKIQYSVTWKEEGDEAADKNVELIREMYE 480
           KRPALTFNPYGGKMSQIPETETPFPHRAGNKYKIQYSVTWKEEGDEAA K++E+IRE+Y+
Sbjct: 421 KRPALTFNPYGGKMSQIPETETPFPHRAGNKYKIQYSVTWKEEGDEAAAKHLEMIRELYK 480

Query: 481 YMTPYVSKSPRSAYLNYRDVDLGVNGNGHA-----SSWGRKYFKGNFDRLVKVKSMVDPD 540
           YMTPYVSKSPRSAYLNYRDVDLGVNG G+A     S WGRKYFKGNFDRLVKVKSMVDPD
Sbjct: 481 YMTPYVSKSPRSAYLNYRDVDLGVNGIGNASYWEGSIWGRKYFKGNFDRLVKVKSMVDPD 540

Query: 541 NFFRYEQSIPCV--EAFDSNGRAESL 557
           NFF+YEQSIPCV  EAFDSNGRAESL
Sbjct: 541 NFFKYEQSIPCVELEAFDSNGRAESL 566

BLAST of Bhi03G000165 vs. NCBI nr
Match: XP_004134895.1 (PREDICTED: reticuline oxidase-like protein [Cucumis sativus])

HSP 1 Score: 968.8 bits (2503), Expect = 8.0e-279
Identity = 485/536 (90.49%), Postives = 504/536 (94.03%), Query Frame = 0

Query: 28  IPVLTTFLLILPSMAVSDQIPSHQTLLKCLSDHSAPSSSPISEVIFFPDNPSYSPVLNSY 87
           IP+LTT LLIL SMA+S Q PSHQTLL CL DHS+PSSSPISEV FFPDNPSYSPVLNSY
Sbjct: 3   IPILTTLLLILSSMALSAQFPSHQTLLHCLFDHSSPSSSPISEVTFFPDNPSYSPVLNSY 62

Query: 88  IRNLRFASPTTPKPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGLSYVSDSTF 147
           IRNLRF SPTTPKPLFIV PTHVSH+QAT+LCCRIHALEIRIRSGGHDYDGLSYVS S F
Sbjct: 63  IRNLRFTSPTTPKPLFIVTPTHVSHVQATVLCCRIHALEIRIRSGGHDYDGLSYVSASPF 122

Query: 148 VILDMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTVGVGGHLSG 207
           VILDMFNLRSVAVDIEDESAWVDSGATLGEVY+KIAEKSKIHGFPAGVCPTVGVGGHLSG
Sbjct: 123 VILDMFNLRSVAVDIEDESAWVDSGATLGEVYYKIAEKSKIHGFPAGVCPTVGVGGHLSG 182

Query: 208 AGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRASMGEDLFWAIRGGGGASFGVIVSWKFK 267
           AGYGNLMRKFGVSVDNVVDALIVDVNGRVLDR +MGEDLFWAIRGGGGASFGVIVSWKFK
Sbjct: 183 AGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRETMGEDLFWAIRGGGGASFGVIVSWKFK 242

Query: 268 LVPLPETVTVFRTEKTMEEGVVDILYKWQEIADKIDENLFIRVVILPVNKKALKTAKAKF 327
           LV LPETVTVFRTEKT+EEGVVDIL+KWQEIAD IDENLFIRVVILPVNKK  KTAKAKF
Sbjct: 243 LVLLPETVTVFRTEKTIEEGVVDILHKWQEIADTIDENLFIRVVILPVNKKTQKTAKAKF 302

Query: 328 ISLFLGNAQKLFALMSKKLPELGIKVEDCIEMSWIDSVLFWSNYPIGTPLNVLLERQPKS 387
           +SLFLGNAQKLFALMS++ PELGIK EDC EMSWI+S+LFWSNYPIGTPLNVLLERQP S
Sbjct: 303 VSLFLGNAQKLFALMSERFPELGIKDEDCKEMSWIESILFWSNYPIGTPLNVLLERQPNS 362

Query: 388 EKFLKKKSDYVQEPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPHRAGN 447
           EKFLKKKSDYVQEPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPHRAGN
Sbjct: 363 EKFLKKKSDYVQEPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPHRAGN 422

Query: 448 KYKIQYSVTWKEEGDEAADKNVELIREMYEYMTPYVSKSPRSAYLNYRDVDLGVNGNGHA 507
           KYKIQYSVTWKEEGDEAA KN++ IRE+Y+YMTPYVSKSPRSAYLNYRDVDLGVNG G+A
Sbjct: 423 KYKIQYSVTWKEEGDEAAAKNLKKIRELYKYMTPYVSKSPRSAYLNYRDVDLGVNGIGNA 482

Query: 508 -----SSWGRKYFKGNFDRLVKVKSMVDPDNFFRYEQSIPC--VEAFDSNGRAESL 557
                S WGRKYFKGNFDRLVKVKSMVDPDNFFRYEQSIPC  VEA DSNGRAESL
Sbjct: 483 SYWVGSIWGRKYFKGNFDRLVKVKSMVDPDNFFRYEQSIPCVEVEALDSNGRAESL 538

BLAST of Bhi03G000165 vs. NCBI nr
Match: XP_022925684.1 (berberine bridge enzyme-like 8 isoform X1 [Cucurbita moschata])

HSP 1 Score: 967.6 bits (2500), Expect = 1.8e-278
Identity = 480/542 (88.56%), Postives = 507/542 (93.54%), Query Frame = 0

Query: 20  MGIPNSLLIPVLTTFLLILPSMAVSDQIPSHQTLLKCLSDHSAPSSSPISEVIFFPDNPS 79
           M IP SLLIP+LTT LLILPSMA +    SH TLL CLSDHS+PS+SPISEV FFPDNPS
Sbjct: 1   MAIPTSLLIPILTTLLLILPSMAAA----SHHTLLSCLSDHSSPSASPISEVTFFPDNPS 60

Query: 80  YSPVLNSYIRNLRFASPTTPKPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGL 139
           YSPVLNSYIRNLRFASPTTPKPLFIVAPTH+SHIQA+ILCCRIHALE+RIRSGGHDYDGL
Sbjct: 61  YSPVLNSYIRNLRFASPTTPKPLFIVAPTHISHIQASILCCRIHALELRIRSGGHDYDGL 120

Query: 140 SYVSDSTFVILDMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTV 199
           SYVSDS FVILDMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTV
Sbjct: 121 SYVSDSPFVILDMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTV 180

Query: 200 GVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRASMGEDLFWAIRGGGGASFG 259
           GVGGHLSGAGYGNLMRKFGVSVDNVVDALIVD NGR LDR SMGEDLFWAIRGGGGASFG
Sbjct: 181 GVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDSNGRALDRESMGEDLFWAIRGGGGASFG 240

Query: 260 VIVSWKFKLVPLPETVTVFRTEKTMEEGVVDILYKWQEIADKIDENLFIRVVILPVNKKA 319
           VIVSWKF+LVPLPETV+VFR EKTMEEG VD+LYKWQE+ADKIDENLFIRVVILPVNKK 
Sbjct: 241 VIVSWKFRLVPLPETVSVFRMEKTMEEGAVDMLYKWQEVADKIDENLFIRVVILPVNKKT 300

Query: 320 LKTAKAKFISLFLGNAQKLFALMSKKLPELGIKVEDCIEMSWIDSVLFWSNYPIGTPLNV 379
            KTAKAKFISLFLG+AQKLF+LMS+ LPELG+K EDC+EMSWI+SVLFWSNYPIGTPLNV
Sbjct: 301 QKTAKAKFISLFLGSAQKLFSLMSETLPELGVKAEDCLEMSWIESVLFWSNYPIGTPLNV 360

Query: 380 LLERQPKSEKFLKKKSDYVQEPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETET 439
           LLERQP SEKFLKKKSDYVQEPISKADLEGM+RKM+ELKRPALTFNPYGGKMS+I E ET
Sbjct: 361 LLERQPNSEKFLKKKSDYVQEPISKADLEGMLRKMMELKRPALTFNPYGGKMSEIAERET 420

Query: 440 PFPHRAGNKYKIQYSVTWKEEGDEAADKNVELIREMYEYMTPYVSKSPRSAYLNYRDVDL 499
           PFPHRAGNKYKIQYSVTWKEEG+EAADKNVELIRE+YEYMTP+VSKSPRSAYLNYRDVDL
Sbjct: 421 PFPHRAGNKYKIQYSVTWKEEGEEAADKNVELIRELYEYMTPHVSKSPRSAYLNYRDVDL 480

Query: 500 GVNGNGH-----ASSWGRKYFKGNFDRLVKVKSMVDPDNFFRYEQSIPCVEAFDSNGRAE 557
           GVNGNG+     AS WGRKYFKGN DRLV+VK+MVDP NFFRYEQSIP   A+DSNGRAE
Sbjct: 481 GVNGNGNASYWEASHWGRKYFKGNLDRLVEVKTMVDPGNFFRYEQSIPSAAAYDSNGRAE 538

BLAST of Bhi03G000165 vs. NCBI nr
Match: XP_022977474.1 (berberine bridge enzyme-like 8 isoform X1 [Cucurbita maxima])

HSP 1 Score: 965.3 bits (2494), Expect = 8.9e-278
Identity = 478/542 (88.19%), Postives = 507/542 (93.54%), Query Frame = 0

Query: 20  MGIPNSLLIPVLTTFLLILPSMAVSDQIPSHQTLLKCLSDHSAPSSSPISEVIFFPDNPS 79
           M IP SLLIP+LTT LLILPSMA +    SH TLL CLSDHS+PS+SPISEV FFPDNPS
Sbjct: 1   MAIPTSLLIPILTTLLLILPSMAAA----SHHTLLSCLSDHSSPSASPISEVTFFPDNPS 60

Query: 80  YSPVLNSYIRNLRFASPTTPKPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGL 139
           YSPVLNSYIRNLRFASPTTPKPLFIVAPTH+SHIQA+ILCCRIHALE+RIRSGGHDYDGL
Sbjct: 61  YSPVLNSYIRNLRFASPTTPKPLFIVAPTHISHIQASILCCRIHALELRIRSGGHDYDGL 120

Query: 140 SYVSDSTFVILDMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTV 199
           SYVSDS FVILDMFNLRSV VDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTV
Sbjct: 121 SYVSDSPFVILDMFNLRSVDVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTV 180

Query: 200 GVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRASMGEDLFWAIRGGGGASFG 259
           GVGGHLSGAGYGNLMRKFGVSVDNVVDALIVD NGRVLDR SMGEDLFWAIRGGGGASFG
Sbjct: 181 GVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDSNGRVLDRESMGEDLFWAIRGGGGASFG 240

Query: 260 VIVSWKFKLVPLPETVTVFRTEKTMEEGVVDILYKWQEIADKIDENLFIRVVILPVNKKA 319
           VIVSWKFKLVPLPETV+VFR EKTMEEG VD+LYKWQE+ADKIDENLFIRVVILPVNKK 
Sbjct: 241 VIVSWKFKLVPLPETVSVFRIEKTMEEGAVDMLYKWQEVADKIDENLFIRVVILPVNKKT 300

Query: 320 LKTAKAKFISLFLGNAQKLFALMSKKLPELGIKVEDCIEMSWIDSVLFWSNYPIGTPLNV 379
            KTAKAKFISLFLG+AQKL++L+S+ LPELG+K EDC+EMSWI+SVLFWSNYPIGTPLNV
Sbjct: 301 QKTAKAKFISLFLGSAQKLYSLISETLPELGVKAEDCLEMSWIESVLFWSNYPIGTPLNV 360

Query: 380 LLERQPKSEKFLKKKSDYVQEPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETET 439
           LLERQP SEKFLKKKSDYVQEPISKADLEGM+RKM+ELKRPALTFNPYGGKMS+I E+ET
Sbjct: 361 LLERQPNSEKFLKKKSDYVQEPISKADLEGMLRKMMELKRPALTFNPYGGKMSEIAESET 420

Query: 440 PFPHRAGNKYKIQYSVTWKEEGDEAADKNVELIREMYEYMTPYVSKSPRSAYLNYRDVDL 499
           PFPHRAGNKYKIQYSVTWKEEG+EAADKNVELIRE+YEYMTP+VSKSPRSAYLNYRDVDL
Sbjct: 421 PFPHRAGNKYKIQYSVTWKEEGEEAADKNVELIRELYEYMTPHVSKSPRSAYLNYRDVDL 480

Query: 500 GVNGNGH-----ASSWGRKYFKGNFDRLVKVKSMVDPDNFFRYEQSIPCVEAFDSNGRAE 557
           GVNGNG+     AS WGRKYFKGNFDRLV+VK+MVDP NFFRYEQSIP   A+D NGR E
Sbjct: 481 GVNGNGNASYWEASQWGRKYFKGNFDRLVEVKTMVDPGNFFRYEQSIPSAAAYDCNGRGE 538

BLAST of Bhi03G000165 vs. NCBI nr
Match: XP_023544585.1 (berberine bridge enzyme-like 8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 961.8 bits (2485), Expect = 9.8e-277
Identity = 479/542 (88.38%), Postives = 505/542 (93.17%), Query Frame = 0

Query: 20  MGIPNSLLIPVLTTFLLILPSMAVSDQIPSHQTLLKCLSDHSAPSSSPISEVIFFPDNPS 79
           M IP SLLIP+LTT LLILPSMA +    SH TLL CLSDHS+PS+SPISEV FFPDNPS
Sbjct: 1   MEIPTSLLIPILTTLLLILPSMAAA----SHHTLLSCLSDHSSPSASPISEVTFFPDNPS 60

Query: 80  YSPVLNSYIRNLRFASPTTPKPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGL 139
           YSPVLNSYIRNLRFASPTTPKPLFIVAPTHVSHIQA+ILCCRIHALE+RIRSGGHDYDGL
Sbjct: 61  YSPVLNSYIRNLRFASPTTPKPLFIVAPTHVSHIQASILCCRIHALELRIRSGGHDYDGL 120

Query: 140 SYVSDSTFVILDMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTV 199
           SYVSDS FVILDMFNLRSVAVDIED SAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTV
Sbjct: 121 SYVSDSPFVILDMFNLRSVAVDIEDGSAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTV 180

Query: 200 GVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRASMGEDLFWAIRGGGGASFG 259
           GVGGHLSGAGYGNLMRKFGVSVDNVVDALIVD NGRVLDR SMGEDLFWAIRGGGGASFG
Sbjct: 181 GVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDSNGRVLDRESMGEDLFWAIRGGGGASFG 240

Query: 260 VIVSWKFKLVPLPETVTVFRTEKTMEEGVVDILYKWQEIADKIDENLFIRVVILPVNKKA 319
           VIVSWKFKLVPLPETV+VFR EKTMEEG VD+LYKWQE+ADKIDENLFIRVVILPVNKK 
Sbjct: 241 VIVSWKFKLVPLPETVSVFRMEKTMEEGAVDMLYKWQEVADKIDENLFIRVVILPVNKKT 300

Query: 320 LKTAKAKFISLFLGNAQKLFALMSKKLPELGIKVEDCIEMSWIDSVLFWSNYPIGTPLNV 379
            KTAKAKFISLFLG+AQKLF+LMS+ LPELG+K EDC+EMSWI+SVLFWSNYP GTPLNV
Sbjct: 301 QKTAKAKFISLFLGSAQKLFSLMSETLPELGVKAEDCLEMSWIESVLFWSNYPTGTPLNV 360

Query: 380 LLERQPKSEKFLKKKSDYVQEPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETET 439
           LLERQP SEKFLKKKSDYVQEPISKADLEGMMRKM+ELKRPALTFNPYGGKMS+I E+ET
Sbjct: 361 LLERQPNSEKFLKKKSDYVQEPISKADLEGMMRKMMELKRPALTFNPYGGKMSEIAESET 420

Query: 440 PFPHRAGNKYKIQYSVTWKEEGDEAADKNVELIREMYEYMTPYVSKSPRSAYLNYRDVDL 499
           PFPHRAGN+YKIQYSVTWKEEG+EAADKNV LIRE+YEYMTP+VSKSPRSAYLNYRDVDL
Sbjct: 421 PFPHRAGNRYKIQYSVTWKEEGEEAADKNVGLIRELYEYMTPHVSKSPRSAYLNYRDVDL 480

Query: 500 GVNGNGH-----ASSWGRKYFKGNFDRLVKVKSMVDPDNFFRYEQSIPCVEAFDSNGRAE 557
           GVNGNG+     AS WGRKYFKGNFDRLV+VK+MVDP NFFRYEQSIP   A+D NGRAE
Sbjct: 481 GVNGNGNASYWEASHWGRKYFKGNFDRLVEVKTMVDPGNFFRYEQSIPSAAAYDCNGRAE 538