Bhi02G001708 (gene) Wax gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Bhi02G001708
Type	gene
Organism	Benincasa hispida (Wax gourd)
Description	Pectinesterase inhibitor, putative
Location	chr2 : 56235071 .. 56235637 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAACCCATTTCAATTGTCATGGTTGTCTTAGTTCTTTCACTTTGCCAGTTCCAAATACTTTCCCAAACTCCACAAACTCCATCAACTTCTGGAGGCAATGATCTCATCACCAAAACCTGCAGCAGCACCCCTTACAATGATATGTGCAAATCAATCTTACTATCATCTCCTAACAGCAATGGAGCAGATCTCTATGGCCTAGCACAAATTGTAATACAAATTGCAGCAGAGAATGTTAGCAACATATATGAAAGCATAAACCAATTACAAAATGGTACATCGGTAGACAGTTTCTTGGATGACTGTTTGACAGATTGCTTGGAGAGCTATCAGGATGCCATTGATCAAATTGAAGATTCAGTAACAGCATTGGAATTCAAAGCCTATAATGATGTTAAGACATGGGTTTCAGCTGCCATGAGTGATGCAGCTACATGTGATATGGGGTTCAAAGAAAAACCAGGCTATCAATCTCCGATTGCACAAATGACTGCAGTTTTTGATCAGATTTGCAACATTATCTTGTCCATGACTCAACTCTTAAGTGAAGAGAAGATTAACTAA

mRNA sequence

ATGAAACCCATTTCAATTGTCATGGTTGTCTTAGTTCTTTCACTTTGCCAGTTCCAAATACTTTCCCAAACTCCACAAACTCCATCAACTTCTGGAGGCAATGATCTCATCACCAAAACCTGCAGCAGCACCCCTTACAATGATATGTGCAAATCAATCTTACTATCATCTCCTAACAGCAATGGAGCAGATCTCTATGGCCTAGCACAAATTGTAATACAAATTGCAGCAGAGAATGTTAGCAACATATATGAAAGCATAAACCAATTACAAAATGGTACATCGGTAGACAGTTTCTTGGATGACTGTTTGACAGATTGCTTGGAGAGCTATCAGGATGCCATTGATCAAATTGAAGATTCAGTAACAGCATTGGAATTCAAAGCCTATAATGATGTTAAGACATGGGTTTCAGCTGCCATGAGTGATGCAGCTACATGTGATATGGGGTTCAAAGAAAAACCAGGCTATCAATCTCCGATTGCACAAATGACTGCAGTTTTTGATCAGATTTGCAACATTATCTTGTCCATGACTCAACTCTTAAGTGAAGAGAAGATTAACTAA

Coding sequence (CDS)

ATGAAACCCATTTCAATTGTCATGGTTGTCTTAGTTCTTTCACTTTGCCAGTTCCAAATACTTTCCCAAACTCCACAAACTCCATCAACTTCTGGAGGCAATGATCTCATCACCAAAACCTGCAGCAGCACCCCTTACAATGATATGTGCAAATCAATCTTACTATCATCTCCTAACAGCAATGGAGCAGATCTCTATGGCCTAGCACAAATTGTAATACAAATTGCAGCAGAGAATGTTAGCAACATATATGAAAGCATAAACCAATTACAAAATGGTACATCGGTAGACAGTTTCTTGGATGACTGTTTGACAGATTGCTTGGAGAGCTATCAGGATGCCATTGATCAAATTGAAGATTCAGTAACAGCATTGGAATTCAAAGCCTATAATGATGTTAAGACATGGGTTTCAGCTGCCATGAGTGATGCAGCTACATGTGATATGGGGTTCAAAGAAAAACCAGGCTATCAATCTCCGATTGCACAAATGACTGCAGTTTTTGATCAGATTTGCAACATTATCTTGTCCATGACTCAACTCTTAAGTGAAGAGAAGATTAACTAA

Protein sequence

MKPISIVMVVLVLSLCQFQILSQTPQTPSTSGGNDLITKTCSSTPYNDMCKSILLSSPNSNGADLYGLAQIVIQIAAENVSNIYESINQLQNGTSVDSFLDDCLTDCLESYQDAIDQIEDSVTALEFKAYNDVKTWVSAAMSDAATCDMGFKEKPGYQSPIAQMTAVFDQICNIILSMTQLLSEEKIN

Homology

[Close]

BLAST of Bhi02G001708 vs. Swiss-Prot
Match: sp|Q8GT41|PLA1_PLAAC (Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.5e-10
Identity = 46/154 (29.87%), Postives = 73/154 (47.40%), Query Frame = 0


Query: 35  DLITKTCSSTPYN------DMCKSILLSSPNSNGADLYGLAQIVIQIAAENVSNIYESIN 94
           D++  TC            D C   L + P S+ ADL GL  I   +A ++ S I   I 
Sbjct: 25  DIVQGTCKKVAQRSPNVNYDFCVKSLGADPKSHTADLQGLGVISANLAIQHGSKIQTFIG 84

Query: 95  QLQNGTSVDSFLXXXXXXXXESYQDAIDQIEDSVTALEFKAYNDVKTWVSAAMSDAATCD 154
           ++   + VD  L          Y DA   +++++   + K Y      +SAA+ D+ TC+
Sbjct: 85  RILK-SKVDPALKKYLNDCVGLYADAKSSVQEAIADFKSKDYASANVKMSAALDDSVTCE 144

Query: 155 MGFKEKPGYQSPIAQMTAVFDQICNIILSMTQLL 183
            GFKEK G  SP+ +    + Q+  I L++T+LL
Sbjct: 145 DGFKEKKGIVSPVTKENKDYVQLTAISLAITKLL 177

[Close]

BLAST of Bhi02G001708 vs. Swiss-Prot
Match: sp|Q9LUV1|PMEI2_ARATH (Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 3.5e-04
Identity = 38/149 (25.50%), Postives = 62/149 (41.61%), Query Frame = 0


Query: 37  ITKTCSSTPYNDMCKSILLSSPNSNGADLYGLAQIVIQIAAENVSNIYESINQLQNGTSV 96
           I   C        C S + S+P ++GADL  LA I    A  + S  +  I  L   T+ 
Sbjct: 30  IKAICGKAKNQSFCTSYMKSNPKTSGADLQTLANITFGSAQTSASEGFRKIQSLVK-TAT 89

Query: 97  DSFLXXXXXXXXESYQDAIDQIEDSVTALEFKAYNDVKTWVSAAMSDAATCDM---GFKE 156
           +  +        + Y+ AI  + D+  +L       +   VSAAM   +TC+     FK 
Sbjct: 90  NPTMKKAYTSCVQHYKSAISSLNDAKQSLASGDGKGLNIKVSAAMEGPSTCEQDMADFKV 149

Query: 157 KPGYQSPIAQMTAVFDQICNIILSMTQLL 183
            P       + +  F  IC I+L ++ ++
Sbjct: 150 DPS----AVKNSGDFQNICGIVLVISNMM 173

[Close]

BLAST of Bhi02G001708 vs. TAIR10
Match: AT3G47670.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 62.8 bits (151), Expect = 2.6e-10
Identity = 47/167 (28.14%), Postives = 82/167 (49.10%), Query Frame = 0


Query: 4   ISIVMVVLVLSLCQFQILSQTPQTPSTSGGNDLITKTCSSTPYNDMCKSILLSSPNSNGA 63
           +S+++++ V++L         P  P  S  +  +   CS T Y + C + L SS N + +
Sbjct: 82  VSLLLILSVVALTAGAFTHSPPHHPPISSAS--LKSLCSVTRYPETCFNSLSSSLNESDS 141

Query: 64  DL--YGLAQIVIQIAAENVSNI---YESINQLQNGTSVDSFLXXXXXXXXESYQDAIDQI 123
            L    + ++ +++AA+ +SN+   + SIN +    +V   +        + Y DA+ Q+
Sbjct: 142 KLNPESILELSLRVAAKEISNLSISFRSINDMPEDAAVGDCV--------KLYTDALSQL 201

Query: 124 EDSVTALEFK------------AYNDVKTWVSAAMSDAATCDMGFKE 154
            DS+T +E K               DVKTW+SAAM+D  TC  G +E
Sbjct: 202 NDSITEIERKKKKGGNNWLTEEVVGDVKTWISAAMTDGETCSDGIEE 238

[Close]

BLAST of Bhi02G001708 vs. TAIR10
Match: AT1G70720.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 56.6 bits (135), Expect = 1.9e-08
Identity = 53/189 (28.04%), Postives = 89/189 (47.09%), Query Frame = 0


Query: 8   MVVLVLSLCQFQILSQTPQTPSTSGGNDLITKTCSSTPYNDMCKSILLSSPNSNGADLYG 67
           +++L+ S    QIL+  P     S G+D I   C++T Y D+C S L S  NS   D   
Sbjct: 9   LLILLFSTFLPQILTVDPPL-LPSNGSDFIRLACNTTLYPDLCFSTLSSFANSIQNDSNR 68

Query: 68  LAQIVIQIAAENVSNIYESINQLQNGTSVDSFLXXXXXXXXESYQDAID----------- 127
           LA++ I +   N  ++   ++ LQN  + D           E+ +DA+D           
Sbjct: 69  LARVAISLTLHNTLHL---LSYLQNAYNRD-HPTPVLRDCFENLKDAVDGMRGSMKQMKE 128

Query: 128 --QIEDSVTALEFKAYNDVKTWVSAAMSDAATCDMGFKEKPGYQSPIAQMTAVFDQICNI 184
                 S+ +  F+  N VKTW+SAA++D  TC  GFK+       + +  ++ D +C+ 
Sbjct: 129 LVSASGSIESFRFQMSN-VKTWLSAALTDEYTCTDGFKD-------VHEDDSIKDDVCSR 184

[Close]

BLAST of Bhi02G001708 vs. TAIR10
Match: AT4G02250.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 54.3 bits (129), Expect = 9.4e-08
Identity = 33/134 (24.63%), Postives = 63/134 (47.01%), Query Frame = 0


Query: 50  CKSILLSSPNSNGADLYGLAQIVIQIAAENVSNIYESINQLQNGTSVDSFLXXXXXXXXE 109
           C + L S P +  A    L  I + IA+ N S+    I       +++  L        +
Sbjct: 26  CVASLTSRPEAATATAPKLGVIALSIASSNASDTSFYIKAKLKQKNLEPALEDTLDDCSK 85

Query: 110 SYQDAIDQIEDSVTALEFKAYNDVKTWVSAAMSDAATCDMGFKEKPGYQSPIAQMTAVFD 169
           +Y DA+ Q++DS+ AL   ++ DV  W++ A+SD   C+    ++ G  + +A+      
Sbjct: 86  NYLDAVAQLDDSLAALMQNSFIDVDIWLNTAISDGEACENALNDRAGNDAELARRNTNLL 145

Query: 170 QICNIILSMTQLLS 184
           ++C   L +  +L+
Sbjct: 146 KLCKDALLINTILT 159

[Close]

BLAST of Bhi02G001708 vs. TAIR10
Match: AT5G46940.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 54.3 bits (129), Expect = 9.4e-08
Identity = 45/173 (26.01%), Postives = 81/173 (46.82%), Query Frame = 0


Query: 12  VLSLCQFQILSQTPQTPSTSGGNDLITKTCSSTPYN-DMCKSILLSSPNSNGA-DLYGLA 71
           +L L  F +LS       T   N     T +S  +  ++C + L ++P +  A DL GL 
Sbjct: 4   LLYLVTFFVLSNGLANGQTLIRNSCKKATATSPKFKYNLCVTSLETNPQAKTAKDLAGLV 63

Query: 72  QIVIQIAAENVSNIYESINQLQNGTSVDSFLXXXXXXXXESYQDAIDQIEDSVTALEFKA 131
               + A    + +  +++++  G  V+           + Y DAI  + +++  ++ + 
Sbjct: 64  MASTKNAVTKATTLKGTVDKIIKGKKVNKMTAMPLRDCLQLYTDAIGSLNEALAGVKSRN 123

Query: 132 YNDVKTWVSAAMSDAATCDMGFKEKPGYQSPIAQMTAVFDQICNIILSMTQLL 183
           Y  VKT +SAAM   +TC+ GFKE+    SP+ +      Q+  I L+ T +L
Sbjct: 124 YPTVKTVLSAAMDTPSTCETGFKERKA-PSPVTKENDNLYQMILIPLAFTNML 175

[Close]

BLAST of Bhi02G001708 vs. TAIR10
Match: AT5G38610.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 50.8 bits (120), Expect = 1.0e-06
Identity = 44/135 (32.59%), Postives = 67/135 (49.63%), Query Frame = 0


Query: 26  QTPSTSGGNDLITKTCS--STPYN-DMCKSILLSSPNS--NGADLYGLAQIVIQIAAENV 85
           Q  STS   D   K+C+  ST ++ + C S L +SP +  +  +L  LA + +  A +N 
Sbjct: 18  QVKSTSDMIDQTCKSCAAKSTIFDYNFCVSSLNNSPIALPSPTNLSSLALVPMLQALDNA 77

Query: 86  SNIYESINQLQNGTSVDSFLXXXXXXXXESYQDAIDQIEDSVTA-LEFKAYNDVKTWVSA 145
           +    +I QL       SF         E Y+DA D++E++V   +  K    V   VSA
Sbjct: 78  TATASTIQQLLISDDDGSFRSACLRDCLELYEDATDRLEEAVRVFITRKELGTVNVMVSA 137

Query: 146 AMSDAATCDMGFKEK 155
           AM  A TC+ GF+E+
Sbjct: 138 AMESAVTCENGFRER 152

[Close]

BLAST of Bhi02G001708 vs. TrEMBL
Match: tr|A0A0A0M1H8|A0A0A0M1H8_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G569235 PE=4 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 1.6e-73
Identity = 145/188 (77.13%), Postives = 165/188 (87.77%), Query Frame = 0


Query: 1   MKPISIVMVVLVLSLCQFQILSQTPQTPSTSGGNDLITKTCSSTPYNDMCKSILLSSPNS 60
           MK I+I +  LV+SLCQFQIL+QTPQTP+TSGGNDLI+KTCSST Y++MCK+IL SSPNS
Sbjct: 1   MKSIAIAVAFLVVSLCQFQILAQTPQTPTTSGGNDLISKTCSSTSYSEMCKTILQSSPNS 60

Query: 61  NGADLYGLAQIVIQIAAENVSNIYESINQLQNGTSVDSFLXXXXXXXXESYQDAIDQIED 120
            GADLYGLAQIV+ +AA+NVS+IYE+INQLQNGTSVDSFL        ES+QDAIDQIED
Sbjct: 61  KGADLYGLAQIVMNVAADNVSSIYENINQLQNGTSVDSFLDSCLTDCLESFQDAIDQIED 120

Query: 121 SVTALEFKAYNDVKTWVSAAMSDAATCDMGFKEKPGYQSPIAQMTAVFDQICNIILSMTQ 180
           SVTALEFKAYNDVKTW+SAAMSD ATCD GFKEK GYQSPIAQMT+VFDQIC+IILS+ Q
Sbjct: 121 SVTALEFKAYNDVKTWISAAMSDVATCDSGFKEKQGYQSPIAQMTSVFDQICSIILSINQ 180

Query: 181 LLSEEKIN 189
           LLS+   N
Sbjct: 181 LLSQGNTN 188

[Close]

BLAST of Bhi02G001708 vs. TrEMBL
Match: tr|A0A1S3BQL1|A0A1S3BQL1_CUCME (putative invertase inhibitor OS=Cucumis melo OX=3656 GN=LOC103492143 PE=4 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 1.5e-60
Identity = 125/153 (81.70%), Postives = 135/153 (88.24%), Query Frame = 0


Query: 1   MKPISIVMVVLVLSLCQFQILSQTPQTPSTSGGNDLITKTCSSTPYNDMCKSILLSSPNS 60
           MK I+I +  LV+SLCQFQIL+QTPQTP+TSGGNDLITKTCSSTP+ +MCK+IL SSPNS
Sbjct: 1   MKSIAIAVAFLVISLCQFQILAQTPQTPTTSGGNDLITKTCSSTPHVEMCKTILQSSPNS 60

Query: 61  NGADLYGLAQIVIQIAAENVSNIYESINQLQNGTSVDSFLXXXXXXXXESYQDAIDQIED 120
            GADLYGLAQIV+ IAA NVSNIYESINQLQNGT+VDSFL        ESYQDAIDQIED
Sbjct: 61  KGADLYGLAQIVMNIAASNVSNIYESINQLQNGTTVDSFLDSCLTDCLESYQDAIDQIED 120

Query: 121 SVTALEFKAYNDVKTWVSAAMSDAATCDMGFKE 154
           SVTALEFKAYNDV TWVSAAMSDAATCDMGFKE
Sbjct: 121 SVTALEFKAYNDVNTWVSAAMSDAATCDMGFKE 153

[Close]

BLAST of Bhi02G001708 vs. TrEMBL
Match: tr|A0A200PWM0|A0A200PWM0_9MAGN (Pectinesterase inhibitor domain OS=Macleaya cordata OX=56857 GN=BVC80_9091g128 PE=4 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 2.4e-29
Identity = 77/184 (41.85%), Postives = 108/184 (58.70%), Query Frame = 0


Query: 2   KPISIVMVVLVLSLCQ--FQILSQTPQTPSTSGGNDLITKTCSSTPYNDMCKSILLSSPN 61
           +PIS++  +L L+     F I            G+DLI KTCS   Y ++C S L   P+
Sbjct: 5   QPISVIFFLLALTFTHQVFAINFHVGAPNLAPTGDDLIVKTCSHAVYKELCVSTLRDDPD 64

Query: 62  SNGADLYGLAQIVIQIAAENVSNIYESINQLQNGTSVDSFLXXXXXXXXESYQDAIDQIE 121
           S  ADL GLA I I+IA  N S I+  I QL    +   F         E+Y DA+DQ+E
Sbjct: 65  SQEADLAGLASIAIKIAMTNGSEIFNHIGQLLKAANYAPFTQQCLSDCSENYMDAVDQLE 124

Query: 122 DSVTALEFKAYNDVKTWVSAAMSDAATCDMGFKEKPGYQSPIAQMTAVFDQICNIILSMT 181
           DS+ A++ K +NDV TWV AAM+DA +C+ GFKE+ G+QS +    AVF+Q+C+  L++T
Sbjct: 125 DSLAAIDAKGFNDVSTWVQAAMTDAESCEDGFKEEVGHQSLLTDRNAVFNQLCSNALALT 184

Query: 182 QLLS 184
            LL+
Sbjct: 185 NLLA 188

[Close]

BLAST of Bhi02G001708 vs. TrEMBL
Match: tr|A0A2N9GTR2|A0A2N9GTR2_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS30954 PE=4 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 2.2e-27
Identity = 71/183 (38.80%), Postives = 113/183 (61.75%), Query Frame = 0


Query: 1   MKPISIVMVVLVLSLCQFQILSQTPQTPSTSGGNDLITKTCSSTPYNDMCKSILLSSPNS 60
           M PIS + +V++L++C  QIL++         G+D I + C  T Y  +C   L S P S
Sbjct: 1   MNPISKLFLVILLTICPHQILAKE--------GSDFIVQACDHTLYKALCVKTLQSDPES 60

Query: 61  N-GADLYGLAQIVIQIAAENVSNIYESINQLQNGTSVDSFLXXXXXXXXESYQDAIDQIE 120
           +  A+++ LA++V++ AA   + I + I +L   TS D  +        E+YQDAIDQ+E
Sbjct: 61  HAAANIHILAKVVLKHAASEANQILDQITKLHGQTS-DQVIEQCLSDCSENYQDAIDQVE 120

Query: 121 DSVTALEFKAYNDVKTWVSAAMSDAATCDMGFKEKPGYQSPIAQMTAVFDQICNIILSMT 180
           D+  ALE   Y+D+ TW++AAMSDA +C+ GF ++PG++SPI  +   F Q+C+I+L++T
Sbjct: 121 DAQLALESNGYDDLNTWITAAMSDADSCEQGFNDQPGHKSPITDLNNTFSQLCSIVLALT 174

Query: 181 QLL 183
             L
Sbjct: 181 NQL 174

[Close]

BLAST of Bhi02G001708 vs. TrEMBL
Match: tr|B9RFD8|B9RFD8_RICCO (Pectinesterase inhibitor, putative OS=Ricinus communis OX=3988 GN=RCOM_1433910 PE=4 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 1.1e-26
Identity = 73/182 (40.11%), Postives = 116/182 (63.74%), Query Frame = 0


Query: 3   PISIVMVVLVLSLCQFQILSQTPQTPSTSGGNDLITKTCSSTPYNDMCKSILLSSPNSNG 62
           P  I+ +VL+L++        T QT +    +DLI+KTC  T Y D CK++L ++P S+ 
Sbjct: 2   PFPILFLVLLLAV-------PTHQTAT----SDLISKTCDQTLYKDYCKTVLGAAPESDV 61

Query: 63  ADLYGLAQIVIQIAAENVSNIYESINQLQNGTSVDSFLXXXXXXXXESYQDAIDQIEDSV 122
            DL  L +  +++A+ N   I++ I+Q+   ++ D F+        E YQDAIDQ+EDS 
Sbjct: 62  KDLPSLTKYALKMASLNGVKIHKKIDQISK-SNKDEFIQQCLDDCSEIYQDAIDQVEDST 121

Query: 123 TALEFKAYNDVKTWVSAAMSDAATCDMGFKEKPGYQSPIAQMTAVFDQICNIILSMTQLL 182
            A++ K+YNDV TWV+AAM+D+ TC+  FKE+ G +SP+      F+Q+C+IIL+M+ LL
Sbjct: 122 AAVDGKSYNDVNTWVTAAMTDSQTCEDAFKEQDGVKSPLTDDNTKFNQLCSIILTMSNLL 171

Query: 183 SE 185
           ++
Sbjct: 182 AK 171

[Close]

BLAST of Bhi02G001708 vs. NCBI nr
Match: XP_011659828.1 (PREDICTED: putative invertase inhibitor [Cucumis sativus] >KGN66046.1 hypothetical protein Csa_1G569235 [Cucumis sativus])

HSP 1 Score: 284.6 bits (727), Expect = 2.4e-73
Identity = 145/188 (77.13%), Postives = 165/188 (87.77%), Query Frame = 0


Query: 1   MKPISIVMVVLVLSLCQFQILSQTPQTPSTSGGNDLITKTCSSTPYNDMCKSILLSSPNS 60
           MK I+I +  LV+SLCQFQIL+QTPQTP+TSGGNDLI+KTCSST Y++MCK+IL SSPNS
Sbjct: 1   MKSIAIAVAFLVVSLCQFQILAQTPQTPTTSGGNDLISKTCSSTSYSEMCKTILQSSPNS 60

Query: 61  NGADLYGLAQIVIQIAAENVSNIYESINQLQNGTSVDSFLXXXXXXXXESYQDAIDQIED 120
            GADLYGLAQIV+ +AA+NVS+IYE+INQLQNGTSVDSFL        ES+QDAIDQIED
Sbjct: 61  KGADLYGLAQIVMNVAADNVSSIYENINQLQNGTSVDSFLDSCLTDCLESFQDAIDQIED 120

Query: 121 SVTALEFKAYNDVKTWVSAAMSDAATCDMGFKEKPGYQSPIAQMTAVFDQICNIILSMTQ 180
           SVTALEFKAYNDVKTW+SAAMSD ATCD GFKEK GYQSPIAQMT+VFDQIC+IILS+ Q
Sbjct: 121 SVTALEFKAYNDVKTWISAAMSDVATCDSGFKEKQGYQSPIAQMTSVFDQICSIILSINQ 180

Query: 181 LLSEEKIN 189
           LLS+   N
Sbjct: 181 LLSQGNTN 188

[Close]

BLAST of Bhi02G001708 vs. NCBI nr
Match: XP_008450606.1 (PREDICTED: putative invertase inhibitor [Cucumis melo])

HSP 1 Score: 241.5 bits (615), Expect = 2.3e-60
Identity = 125/153 (81.70%), Postives = 135/153 (88.24%), Query Frame = 0


Query: 1   MKPISIVMVVLVLSLCQFQILSQTPQTPSTSGGNDLITKTCSSTPYNDMCKSILLSSPNS 60
           MK I+I +  LV+SLCQFQIL+QTPQTP+TSGGNDLITKTCSSTP+ +MCK+IL SSPNS
Sbjct: 1   MKSIAIAVAFLVISLCQFQILAQTPQTPTTSGGNDLITKTCSSTPHVEMCKTILQSSPNS 60

Query: 61  NGADLYGLAQIVIQIAAENVSNIYESINQLQNGTSVDSFLXXXXXXXXESYQDAIDQIED 120
            GADLYGLAQIV+ IAA NVSNIYESINQLQNGT+VDSFL        ESYQDAIDQIED
Sbjct: 61  KGADLYGLAQIVMNIAASNVSNIYESINQLQNGTTVDSFLDSCLTDCLESYQDAIDQIED 120

Query: 121 SVTALEFKAYNDVKTWVSAAMSDAATCDMGFKE 154
           SVTALEFKAYNDV TWVSAAMSDAATCDMGFKE
Sbjct: 121 SVTALEFKAYNDVNTWVSAAMSDAATCDMGFKE 153

[Close]

BLAST of Bhi02G001708 vs. NCBI nr
Match: XP_022135660.1 (putative invertase inhibitor [Momordica charantia])

HSP 1 Score: 238.8 bits (608), Expect = 1.5e-59
Identity = 126/187 (67.38%), Postives = 149/187 (79.68%), Query Frame = 0


Query: 1   MKPISIVMVVLVLSLCQFQILSQTPQ----TPSTSGGNDLITKTCSSTPYNDMCKSILLS 60
           M PISI++ +LVLSLCQFQIL+   Q             LITKTC+STPY D C SIL S
Sbjct: 1   MNPISILVPILVLSLCQFQILADQLQXXXXXXXXXXXXXLITKTCTSTPYKDFCISILQS 60

Query: 61  SPNSNGADLYGLAQIVIQIAAENVSNIYESINQLQNGTSVDSFLXXXXXXXXESYQDAID 120
           SP SNGADLYGLA+I+++ AA+NVS+IYESIN LQN +S D+F+        ES+QDAID
Sbjct: 61  SPKSNGADLYGLAEILMETAAQNVSSIYESINHLQNDSSPDAFMEQCLADCLESFQDAID 120

Query: 121 QIEDSVTALEFKAYNDVKTWVSAAMSDAATCDMGFKEKPGYQSPIAQMTAVFDQICNIIL 180
           QIEDSVTALEFKAYND+KTWVSAAMSDAATCD GFK++ GYQSP+AQMTAVFDQIC+IIL
Sbjct: 121 QIEDSVTALEFKAYNDIKTWVSAAMSDAATCDQGFKDRQGYQSPVAQMTAVFDQICSIIL 180

Query: 181 SMTQLLS 184
           ++T LL+
Sbjct: 181 AVTHLLA 187

[Close]

BLAST of Bhi02G001708 vs. NCBI nr
Match: XP_022970023.1 (pectinesterase inhibitor 7-like [Cucurbita maxima])

HSP 1 Score: 225.7 bits (574), Expect = 1.3e-55
Identity = 115/189 (60.85%), Postives = 145/189 (76.72%), Query Frame = 0


Query: 1   MKPISI-VMVVLVLSLCQFQILSQTPQTPSTSGGNDLITKTCSSTPYNDMCKSILLSSPN 60
           MKPIS+ V V++V+SLC FQ            G N+LITK CS T YND+CK+IL ++PN
Sbjct: 1   MKPISVAVAVLVVVSLCSFQ----------AQGDNELITKACSRTAYNDLCKAILQAAPN 60

Query: 61  SNGADLYGLAQIVIQIAAENVSNIYESINQLQNGTSVDSFLXXXXXXXXESYQDAIDQIE 120
           SN ADLYGL Q  + IAA+NVSNIY++I++LQN +SVDSF+        E+YQDA DQIE
Sbjct: 61  SNDADLYGLTQFALSIAAQNVSNIYQTIDKLQNDSSVDSFMDECLTDCLENYQDATDQIE 120

Query: 121 DSVTALEFKAYNDVKTWVSAAMSDAATCDMGFKEKPGYQSPIAQMTAVFDQICNIILSMT 180
           DSVTALEFKA+NDV+TWV+AAMSD ATCD+GFK+KPGYQSP+ QM+AVFDQIC+I+L+  
Sbjct: 121 DSVTALEFKAFNDVRTWVAAAMSDVATCDLGFKDKPGYQSPVHQMSAVFDQICSIVLTFN 179

Query: 181 QLLSEEKIN 189
             L E  ++
Sbjct: 181 HCLIEGNVH 179

[Close]

BLAST of Bhi02G001708 vs. NCBI nr
Match: XP_022968792.1 (pectinesterase inhibitor-like [Cucurbita maxima])

HSP 1 Score: 221.9 bits (564), Expect = 1.9e-54
Identity = 116/189 (61.38%), Postives = 145/189 (76.72%), Query Frame = 0


Query: 1   MKPISI-VMVVLVLSLCQFQILSQTPQTPSTSGGNDLITKTCSSTPYNDMCKSILLSSPN 60
           MK ISI  M + VLS+  FQIL+  PQ P  +   DLITKTCSSTPYND+C SIL SSPN
Sbjct: 1   MKLISIAAMAITVLSMTHFQILADNPQKPFLTNA-DLITKTCSSTPYNDLCLSILESSPN 60

Query: 61  SNGADLYGLAQIVIQIAAENVSNIYESINQLQNGTSVDSFLXXXXXXXXESYQDAIDQIE 120
           SNG DLYGL +I++ IAAENVS I+E + +LQN T +D+F+        E+YQDA DQIE
Sbjct: 61  SNGTDLYGLTEIMLYIAAENVSLIHEHLMELQNNTEMDTFMDECLTDCLENYQDATDQIE 120

Query: 121 DSVTALEFKAYNDVKTWVSAAMSDAATCDMGFKEKPGYQSPIAQMTAVFDQICNIILSMT 180
           DS+TALEFKAY+D+KTWV+AAM+D A C+ GFKEKP ++SPI +M+ VFDQIC+IIL+MT
Sbjct: 121 DSITALEFKAYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAMT 180

Query: 181 QLLSEEKIN 189
            +LS+   N
Sbjct: 181 NILSQGNRN 188

The following BLAST results are available for this feature:

BLAST of Bhi02G001708 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q8GT41\|PLA1_PLAAC	1.5e-10	29.87	Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1	[more]
sp\|Q9LUV1\|PMEI2_ARATH	3.5e-04	25.50	Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1	[more]

BLAST of Bhi02G001708 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G47670.1	2.6e-10	28.14	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G70720.1	1.9e-08	28.04	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G02250.1	9.4e-08	24.63	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46940.1	9.4e-08	26.01	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G38610.1	1.0e-06	32.59	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

BLAST of Bhi02G001708 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0M1H8\|A0A0A0M1H8_CUCSA	1.6e-73	77.13	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G569235 PE=4 SV=1	[more]
tr\|A0A1S3BQL1\|A0A1S3BQL1_CUCME	1.5e-60	81.70	putative invertase inhibitor OS=Cucumis melo OX=3656 GN=LOC103492143 PE=4 SV=1	[more]
tr\|A0A200PWM0\|A0A200PWM0_9MAGN	2.4e-29	41.85	Pectinesterase inhibitor domain OS=Macleaya cordata OX=56857 GN=BVC80_9091g128 P...	[more]
tr\|A0A2N9GTR2\|A0A2N9GTR2_FAGSY	2.2e-27	38.80	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS30954 PE=4 SV=1	[more]
tr\|B9RFD8\|B9RFD8_RICCO	1.1e-26	40.11	Pectinesterase inhibitor, putative OS=Ricinus communis OX=3988 GN=RCOM_1433910 P...	[more]

BLAST of Bhi02G001708 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011659828.1	2.4e-73	77.13	PREDICTED: putative invertase inhibitor [Cucumis sativus] >KGN66046.1 hypothetic...	[more]
XP_008450606.1	2.3e-60	81.70	PREDICTED: putative invertase inhibitor [Cucumis melo]	[more]
XP_022135660.1	1.5e-59	67.38	putative invertase inhibitor [Momordica charantia]	[more]
XP_022970023.1	1.3e-55	60.85	pectinesterase inhibitor 7-like [Cucurbita maxima]	[more]
XP_022968792.1	1.9e-54	61.38	pectinesterase inhibitor-like [Cucurbita maxima]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004857	enzyme inhibitor activity

Vocabulary: **INTERPRO**
Term	Definition
IPR035513	Invertase/methylesterase_inhib
IPR006501	Pectinesterase_inhib_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi02M001708	Bhi02M001708	mRNA

InterPro

Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 108..128
None	No IPR available	PANTHER	PTHR31080	FAMILY NOT NAMED	coord: 3..183
None	No IPR available	CDD	cd15801	PMEI-like_1	coord: 36..182 e-value: 1.68957E-54 score: 171.36
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 32..178 e-value: 8.8E-33 score: 124.9
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 35..178 e-value: 8.1E-34 score: 117.1
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 6..181 e-value: 5.2E-42 score: 141.1
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	G3DSA:1.20.140.40		coord: 33..184 e-value: 2.9E-46 score: 159.1
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	SSF101148	Plant invertase/pectin methylesterase inhibitor	coord: 34..183

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Bhi02G001708	MELO3C015291	Melon (DHL92) v3.5.1	mewgoB294
Bhi02G001708	ClCG03G017200	Watermelon (Charleston Gray)	wcgwgoB364
Bhi02G001708	CSPI01G26290	Wild cucumber (PI 183967)	cpiwgoB084
Bhi02G001708	Cucsa.181740	Cucumber (Gy14) v1	cgywgoB372
Bhi02G001708	CmoCh11G012440	Cucurbita moschata (Rifu)	cmowgoB0164
Bhi02G001708	Lsi06G000910	Bottle gourd (USVL1VR-Ls)	lsiwgoB507
Bhi02G001708	CsGy1G025550	Cucumber (Gy14) v2	cgybwgoB080
Bhi02G001708	MELO3C015291.2	Melon (DHL92) v3.6.1	medwgoB295
Bhi02G001708	Carg16992	Silver-seed gourd	carwgoB0571
Bhi02G001708	CsaV3_1G038650	Cucumber (Chinese Long) v3	cucwgoB090
Bhi02G001708	Cla005211	Watermelon (97103) v1	wgowmB656
Bhi02G001708	Cla97C03G067560	Watermelon (97103) v2	wgowmbB632

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Bhi02G001708	Cucurbita maxima (Rimu)	cmawgoB0090
Bhi02G001708	Cucurbita maxima (Rimu)	cmawgoB0157
Bhi02G001708	Cucurbita maxima (Rimu)	cmawgoB0236
Bhi02G001708	Cucurbita moschata (Rifu)	cmowgoB0084
Bhi02G001708	Cucurbita moschata (Rifu)	cmowgoB0229
Bhi02G001708	Cucurbita pepo (Zucchini)	cpewgoB0462
Bhi02G001708	Cucurbita pepo (Zucchini)	cpewgoB0472
Bhi02G001708	Cucurbita pepo (Zucchini)	cpewgoB0894
Bhi02G001708	Cucurbita pepo (Zucchini)	cpewgoB1072
Bhi02G001708	Cucumber (Chinese Long) v2	cuwgoB082