Bhi02G001421 (gene) Wax gourd

BLAST of Bhi02G001421 vs. Swiss-Prot
Match: sp|P14133|ASO_CUCSA (L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1)

HSP 1 Score: 1009.6 bits (2609), Expect = 1.4e-293
Identity = 468/582 (80.41%), Postives = 517/582 (88.83%), Query Frame = 0

Query: 1   MAKAANQPLFPLPFLVIFLIFSMISGFGITLSEA-VNNTRYFKWEVEYMFWSPDCVENLV 60
           MAK A++P FP PFL  FL+ S+I GFGITLSEA     +++KW+VEYMFWSPDCVEN+V
Sbjct: 1   MAKVADKPFFPKPFL-SFLVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIV 60

Query: 61  MGINGQFPGPTIRANVGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAIN 120
           MGING+FPGPTIRAN GD ++VEL NKL TEGVVIHWHGILQR TPWADGTASISQCAIN
Sbjct: 61  MGINGEFPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAIN 120

Query: 121 PGETFIYQFKADKPGTYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYDEEINLLLSDW 180
           PGETF Y+F  DK GTYFYHGHLGMQRSAGLYGSLI+DP EG+ EPFHYDEEINLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDW 180

Query: 181 WHKSAHKQEVGLSSSPMRWIGEPETILINGRGQISCSIAAKYNKNLEQCKLKGGEQCAPF 240
           WH+S HKQEVGLSS PMRWIGEP++ILING+GQ  CSIAAKYN+ L+QC+L G E+CAPF
Sbjct: 181 WHQSVHKQEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPF 240

Query: 241 ILQVKPKKTYRIRIASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSV 300
           IL V+PKKTYRIRIASTT+L++LN AIGNH+LLVVEADGNYV+PFVTSDIDIYSGESYSV
Sbjct: 241 ILHVQPKKTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSV 300

Query: 301 LITTNQDPAQNYWVSVGVRGRLPKTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSK 360
           LITT+Q+P +NYWVS+GVR RLPKTPP LTLLNYLPNS +KLP+SPPPETP+WED+ RSK
Sbjct: 301 LITTDQNPLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSK 360

Query: 361 NFTYRILAAMGSPNPPIKFNRRIFLLNTQNRMNGGFTKWAINNVSLSLPSTPYLGAIKIG 420
           NFT+RI AAMGSP PP+++NRR+FLLNTQNR+N GF KWAINNVSL+LP TPYL A+K+ 
Sbjct: 361 NFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRIN-GFMKWAINNVSLALPPTPYLAAMKMR 420

Query: 421 LNNTFNQNPPPETFSEDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANTLNDKHSE 480
           LN  FNQNPPPETF  +YDI NPPPNP T T NGVY FN+GETVDVILQNAN LN   SE
Sbjct: 421 LNTAFNQNPPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSE 480

Query: 481 IHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
           IHPWHLHGHDFWVLGYGEGKF A  DEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540

Query: 541 WAFHCHIEPHLHMGMGVIFAEGVHKVGTIPTKALACGGTAKV 582
           WAFHCHIEPHLHMGMGV+FAEGVH VG IP KALACG TA V
Sbjct: 541 WAFHCHIEPHLHMGMGVVFAEGVHMVGMIPPKALACGSTALV 580

BLAST of Bhi02G001421 vs. Swiss-Prot
Match: sp|P24792|ASO_CUCMA (L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2)

HSP 1 Score: 961.8 bits (2485), Expect = 3.4e-279
Identity = 448/574 (78.05%), Postives = 492/574 (85.71%), Query Frame = 0

Query: 13  PFLVIFLIFSMISGFGITLSEAVNNTRYFKWEVEYMFWSPDCVENLVMGINGQFPGPTIR 72
           P  +I   F +I  FGI  S   +  R++KWEVEYMFW+PDC EN+VMGINGQFPGPTIR
Sbjct: 10  PNFLILFFFGLILAFGI--SSEGSQIRHYKWEVEYMFWAPDCNENIVMGINGQFPGPTIR 69

Query: 73  ANVGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAINPGETFIYQFKADK 132
           AN GDT++VEL+NKL TEGVVIHWHGILQR TPWADGTASISQCAINPGETF Y F  D 
Sbjct: 70  ANAGDTVVVELINKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFFYNFTVDN 129

Query: 133 PGTYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYDEEINLLLSDWWHKSAHKQEVGLS 192
           PGT+FYHGHLGMQRSAGLYGSLI+DP +GKKEPFHYD EINLLLSDWWH+S HKQEVGLS
Sbjct: 130 PGTFFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLS 189

Query: 193 SSPMRWIGEPETILINGRGQISCSIAAKYNKNLEQCKLKGGEQCAPFILQVKPKKTYRIR 252
           S P+RWIGEP+TIL+NGRGQ  CSIAAKY+ NLE CKLKG E CAP+I  V PKKTYRIR
Sbjct: 190 SKPIRWIGEPQTILLNGRGQFDCSIAAKYDSNLEPCKLKGSEPCAPYIFHVMPKKTYRIR 249

Query: 253 IASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSVLITTNQDPAQNYW 312
           IASTT+L+ALN AIGNH LLVVEADGNYV+PF TSDIDIYSGESYSVLITT+Q+P++NYW
Sbjct: 250 IASTTALAALNFAIGNHPLLVVEADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYW 309

Query: 313 VSVGVRGRLPKTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSKNFTYRILAAMGSP 372
           VSVG RGR P TPP LTLLNYLPNSV+KLP SPPPETP W+D+ RSKNFTYRI AAMGSP
Sbjct: 310 VSVGTRGRHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNFTYRITAAMGSP 369

Query: 373 NPPIKFNRRIFLLNTQNRMNGGFTKWAINNVSLSLPSTPYLGAIKIGLNNTFNQNPPPET 432
            PP+K NRRIFLLNTQN +N G+ KWAIN+VSL+LP TPYLGA+K  L + F+QNPPPE 
Sbjct: 370 KPPVKSNRRIFLLNTQNVIN-GYVKWAINDVSLALPPTPYLGAMKFNLLHAFDQNPPPEV 429

Query: 433 FSEDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANTLNDKHSEIHPWHLHGHDFWV 492
           F EDYDI  PP N  T   NGVY F +GE VDVILQNAN + +  SEIHPWHLHGHDFWV
Sbjct: 430 FPEDYDIDTPPTNEKTKIGNGVYQFKIGEIVDVILQNANMMKENLSEIHPWHLHGHDFWV 489

Query: 493 LGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHM 552
           LGYG+GKF+A  +E  LNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHM
Sbjct: 490 LGYGDGKFTAE-EESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHM 549

Query: 553 GMGVIFAEGVHKVGTIPTKALACGGTAKVLINNP 587
           GMGV+FAEGV KVG IPTKALACGGTAK LINNP
Sbjct: 550 GMGVVFAEGVEKVGRIPTKALACGGTAKSLINNP 579

BLAST of Bhi02G001421 vs. Swiss-Prot
Match: sp|P37064|ASO_CUCPM (L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1)

HSP 1 Score: 952.2 bits (2460), Expect = 2.7e-276
Identity = 438/548 (79.93%), Postives = 480/548 (87.59%), Query Frame = 0

Query: 39  RYFKWEVEYMFWSPDCVENLVMGINGQFPGPTIRANVGDTIIVELVNKLFTEGVVIHWHG 98
           R++KWEVEYMFW+P+C EN+VMGINGQFPGPTIRAN GD+++VEL NKL TEGVVIHWHG
Sbjct: 4   RHYKWEVEYMFWAPNCNENIVMGINGQFPGPTIRANAGDSVVVELTNKLHTEGVVIHWHG 63

Query: 99  ILQRETPWADGTASISQCAINPGETFIYQFKADKPGTYFYHGHLGMQRSAGLYGSLIIDP 158
           ILQR TPWADGTASISQCAINPGETF Y F  D PGT+FYHGHLGMQRSAGLYGSLI+DP
Sbjct: 64  ILQRGTPWADGTASISQCAINPGETFFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLIVDP 123

Query: 159 EEGKKEPFHYDEEINLLLSDWWHKSAHKQEVGLSSSPMRWIGEPETILINGRGQISCSIA 218
            +GKKEPFHYD EINLLLSDWWH+S HKQEVGLSS P+RWIGEP+TIL+NGRGQ  CSIA
Sbjct: 124 PQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSSKPIRWIGEPQTILLNGRGQFDCSIA 183

Query: 219 AKYNKNLEQCKLKGGEQCAPFILQVKPKKTYRIRIASTTSLSALNLAIGNHQLLVVEADG 278
           AKY+ NLE CKLKG E CAP+I  V PKKTYRIRIASTT+L+ALN AIGNHQLLVVEADG
Sbjct: 184 AKYDSNLEPCKLKGSESCAPYIFHVSPKKTYRIRIASTTALAALNFAIGNHQLLVVEADG 243

Query: 279 NYVEPFVTSDIDIYSGESYSVLITTNQDPAQNYWVSVGVRGRLPKTPPALTLLNYLPNSV 338
           NYV+PF TSDIDIYSGESYSVLITT+Q+P++NYWVSVG R R P TPP LTLLNYLPNSV
Sbjct: 244 NYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVSVGTRARHPNTPPGLTLLNYLPNSV 303

Query: 339 AKLPVSPPPETPNWEDYARSKNFTYRILAAMGSPNPPIKFNRRIFLLNTQNRMNGGFTKW 398
           +KLP SPPP+TP W+D+ RSKNFTYRI AAMGSP PP+KFNRRIFLLNTQN +N G+ KW
Sbjct: 304 SKLPTSPPPQTPAWDDFDRSKNFTYRITAAMGSPKPPVKFNRRIFLLNTQNVIN-GYVKW 363

Query: 399 AINNVSLSLPSTPYLGAIKIGLNNTFNQNPPPETFSEDYDIFNPPPNPNTITDNGVYMFN 458
           AIN+VSL+LP TPYLGA+K  L + F+QNPPPE F EDYDI  PP N  T   NGVY F 
Sbjct: 364 AINDVSLALPPTPYLGAMKYNLLHAFDQNPPPEVFPEDYDIDTPPTNEKTRIGNGVYQFK 423

Query: 459 LGETVDVILQNANTLNDKHSEIHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRN 518
           +GE VDVILQNAN + +  SE HPWHLHGHDFWVLGYG+GKFSA  +E  LNLKNPPLRN
Sbjct: 424 IGEVVDVILQNANMMKENLSETHPWHLHGHDFWVLGYGDGKFSAE-EESSLNLKNPPLRN 483

Query: 519 TVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVIFAEGVHKVGTIPTKALACGGT 578
           TVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGV+FAEGV KVG IPTKALACGGT
Sbjct: 484 TVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVEKVGRIPTKALACGGT 543

Query: 579 AKVLINNP 587
           AK LINNP
Sbjct: 544 AKSLINNP 549

BLAST of Bhi02G001421 vs. Swiss-Prot
Match: sp|Q40588|ASO_TOBAC (L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1)

HSP 1 Score: 829.3 bits (2141), Expect = 2.6e-239
Identity = 387/575 (67.30%), Postives = 459/575 (79.83%), Query Frame = 0

Query: 12  LPFLVIFLIFSMISGFGITLSEAVNNTRYFKWEVEYMFWSPDCVENLVMGINGQFPGPTI 71
           L FL  FL+  ++     + S     TR+FKW+VEY+ WSPD  E++VMGINGQFPGPTI
Sbjct: 4   LGFLFFFLLPLILLELSSSRSVMAAKTRHFKWDVEYIHWSPDGEESVVMGINGQFPGPTI 63

Query: 72  RANVGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAINPGETFIYQFKAD 131
           RA  GDT+ V L NKL TEGVVIHWHGI Q  TPWADGTA+ISQCAINPGETF+Y+FK D
Sbjct: 64  RAKAGDTVAVHLTNKLHTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGETFLYRFKVD 123

Query: 132 KPGTYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYDEEINLLLSDWWHKSAHKQEVGL 191
           K GTYFYHGH GMQRSAGLYGSLI++  EG+KEPFHYD E NLLLSDWWHK +H+QEV L
Sbjct: 124 KAGTYFYHGHYGMQRSAGLYGSLIVEVGEGEKEPFHYDGEFNLLLSDWWHKGSHEQEVDL 183

Query: 192 SSSPMRWIGEPETILINGRGQISCSIAAKYNK-NLEQCKLKGGEQCAPFILQVKPKKTYR 251
           SS+P+RWIGEP+T+L+NGRGQ +CS+AA+++K  L QCKL+GGEQ AP IL+V+P K YR
Sbjct: 184 SSNPLRWIGEPQTLLLNGRGQYNCSLAARFSKPPLPQCKLRGGEQYAPQILRVRPNKIYR 243

Query: 252 IRIASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSVLITTNQDPAQN 311
           +R+ASTT+L +L+LAIG H+++VVEADGNYV+PF   D+DIYSGESYSVL  T+QDP +N
Sbjct: 244 LRVASTTALGSLSLAIGGHKMVVVEADGNYVQPFSVQDMDIYSGESYSVLFKTDQDPTKN 303

Query: 312 YWVSVGVRGRLPKTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSKNFTYRILAAMG 371
           YW+S+ VRGR PKTP  LTLLNYLPNS +K P  PPP  P W DY  SK+F+ +I A MG
Sbjct: 304 YWISINVRGREPKTPQGLTLLNYLPNSASKFPTLPPPIAPLWNDYNHSKSFSNKIFALMG 363

Query: 372 SPNPPIKFNRRIFLLNTQNRMNGGFTKWAINNVSLSLPSTPYLGAIKIGLNNTFNQNPPP 431
           SP PP + +RRI LLNTQN+++ G+TKWAINNVSL LP+  YLG+I+ G+ N F+  PPP
Sbjct: 364 SPKPPPQNHRRIILLNTQNKID-GYTKWAINNVSLVLPTQLYLGSIRYGI-NAFDTKPPP 423

Query: 432 ETFSEDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANTLNDKHSEIHPWHLHGHDF 491
           + F +DYD+    PN N+   NGVYM     T+D+ILQNAN L    SEIHPWHLHGHDF
Sbjct: 424 DNFPKDYDVLKQAPNSNSTYGNGVYMLKFNTTIDIILQNANALAKDVSEIHPWHLHGHDF 483

Query: 492 WVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHL 551
           WVLGYGEGKFS   D KK NLKNPPLRNT VIFP+GWTA+RFV DNPGVWAFHCHIEPHL
Sbjct: 484 WVLGYGEGKFSEK-DVKKFNLKNPPLRNTAVIFPFGWTALRFVTDNPGVWAFHCHIEPHL 543

Query: 552 HMGMGVIFAEGVHKVGTIPTKALACGGTAKVLINN 586
           HMGMGVIFAEGVH V  IP +ALACG T K+L++N
Sbjct: 544 HMGMGVIFAEGVHLVKKIPKEALACGLTGKMLMSN 575

BLAST of Bhi02G001421 vs. Swiss-Prot
Match: sp|Q8VZA1|LAC11_ARATH (Laccase-11 OS=Arabidopsis thaliana OX=3702 GN=LAC11 PE=2 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 2.2e-68
Identity = 175/566 (30.92%), Postives = 279/566 (49.29%), Query Frame = 0

Query: 17  IFLIFSMISGFGITLSEAVNNTRYFKWEVEYMFWSPDCVENLVMGINGQFPGPTIRANVG 76
           +FL   +++  G +  +A    + ++++V+    S  C    ++ +NG FPGPT+ A  G
Sbjct: 6   LFLFCYLLAFLGYSPVDAA--VKKYQFDVQVKNISRICNAKPIVTVNGMFPGPTVYAREG 65

Query: 77  DTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAINPGETFIYQFK-ADKPGT 136
           D +I+ + N +    + IHWHG+ Q    WADG A I+QC I  G++++Y F    + GT
Sbjct: 66  DRVIINVTNHV-QYNMSIHWHGLKQYRNGWADGPAYITQCPIQTGQSYLYDFNVTGQRGT 125

Query: 137 YFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYD-EEINLLLSDWWHKSAHKQEVGLSSS 196
            ++H H+   R A +YG+++I P  GK  PF    +E N++L +WW+K     +V  + +
Sbjct: 126 LWWHAHILWLR-ATVYGAIVILPAPGKPYPFPQPYQESNIILGEWWNK-----DVETAVN 185

Query: 197 PMRWIGEP----ETILINGRGQISCSIAAKYNKNLEQCKLKGGEQCAPFILQVKPKKTYR 256
               +G P    +   ING+       + K+                 F+++ +  KTY 
Sbjct: 186 QANQLGAPPPMSDAHTINGKPGPLFPCSEKHT----------------FVIEAEAGKTYL 245

Query: 257 IRIASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSVLITTNQDPAQN 316
           +RI +      L   I  H + VVE D  Y +PF T  I +  G++ +VL+ T++ P   
Sbjct: 246 LRIINAALNDELFFGIAGHNMTVVEIDAVYTKPFTTKAILLGPGQTTNVLVKTDRSP-NR 305

Query: 317 YWVSVGVRGRLP-----KTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSKNFTYRI 376
           Y+++       P     KT  A+     +PN+V  LP+ P    PN   +A   N     
Sbjct: 306 YFMAASPFMDAPVSVDNKTVTAILQYKGVPNTV--LPILPKLPLPNDTSFALDYNGK--- 365

Query: 377 LAAMGSPN----PPIKFNRRIF------LLNTQNRMNGGFTKWAINNVSLSLPSTPYLGA 436
           L ++ +PN     P+K +RR+F      +      +NG     +INN++  +P T  L A
Sbjct: 366 LKSLNTPNFPALVPLKVDRRLFYTIGLGINACPTCVNGTNLAASINNITFIMPKTALLKA 425

Query: 437 IKIGLNNTFN---QNPPPETFSEDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANT 496
               ++  F     + PP+ F  +Y       N  T T   +       T++++LQ+ N 
Sbjct: 426 HYSNISGVFRTDFPDRPPKAF--NYTGVPLTANLGTSTGTRLSRVKFNTTIELVLQDTNL 485

Query: 497 LNDKHSEIHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRF 556
           L     E HP+HLHG++F+V+G G G F    D  K NL +PP RNTV +   GW AIRF
Sbjct: 486 LT---VESHPFHLHGYNFFVVGTGVGNFDPKKDPAKFNLVDPPERNTVGVPTGGWAAIRF 535

Query: 557 VADNPGVWAFHCHIEPHLHMGMGVIF 559
            ADNPGVW  HCH+E H   G+ + F
Sbjct: 546 RADNPGVWFMHCHLEVHTMWGLKMAF 535

BLAST of Bhi02G001421 vs. TAIR10
Match: AT5G21105.1 (Plant L-ascorbate oxidase)

HSP 1 Score: 803.5 bits (2074), Expect = 8.6e-233
Identity = 364/557 (65.35%), Postives = 442/557 (79.35%), Query Frame = 0

Query: 30  TLSEAVNNTRYFKWEVEYMFWSPDCVENLVMGINGQFPGPTIRANVGDTIIVELVNKLFT 89
           T S AV   R + WEVEY +WSPDC E  VM +NG+FPGPTI+A  GDTI+V L NKL T
Sbjct: 32  TASAAV---REYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGDTIVVNLTNKLTT 91

Query: 90  EGVVIHWHGILQRETPWADGTASISQCAINPGETFIYQFKADKPGTYFYHGHLGMQRSAG 149
           EG+VIHWHGI Q  +PWADG A ++QCAINPGETF Y F  +KPGT+FYHGH GMQRSAG
Sbjct: 92  EGLVIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTHFYHGHYGMQRSAG 151

Query: 150 LYGSLIIDPEEGKKEPFHYDEEINLLLSDWWHKSAHKQEVGLSSSPMRWIGEPETILING 209
           LYGSLI+D  +GK E   YD E NLLLSDWWH++   QE+GLSS PMRWIGE ++ILING
Sbjct: 152 LYGSLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSSKPMRWIGEAQSILING 211

Query: 210 RGQISCSIAAKYNKN--LEQCKLKGGEQCAPFILQVKPKKTYRIRIASTTSLSALNLAIG 269
           RGQ +CS+AA+++ N  L  C  K G+QCAP IL V+P KTYRIR++STT+L++LNLA+ 
Sbjct: 212 RGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALASLNLAVQ 271

Query: 270 NHQLLVVEADGNYVEPFVTSDIDIYSGESYSVLITTNQDPAQNYWVSVGVRGRLPKTPPA 329
            H+L+VVEADGNY+ PF T DIDIYSGESYSVL+TT+QDP+QNY++SVGVRGR P T  A
Sbjct: 272 GHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGRKPNTTQA 331

Query: 330 LTLLNYLPNSVAKLPVSPPPETPNWEDYARSKNFTYRILAAMGSPNPPIKFNRRIFLLNT 389
           LT+LNY+    +KLP SPPP TP W+D+ RSKNF+ +I +AMGSP+PP K+ +R+ LLNT
Sbjct: 332 LTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSPPKKYRKRLILLNT 391

Query: 390 QNRMNGGFTKWAINNVSLSLPSTPYLGAIKIGLNNTFNQNPPPETFSEDYDIFNPPPNPN 449
           QN ++ G+TKWAINNVSL  P+TPYLG++K  L   FN+  PP ++  DYDI NPPP PN
Sbjct: 392 QNLID-GYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDYDIMNPPPFPN 451

Query: 450 TITDNGVYMFNLGETVDVILQNANTLNDKHSEIHPWHLHGHDFWVLGYGEGKFSAAVDEK 509
           T T NG+Y+F    TVDVI+QNAN L    SEIHPWHLHGHDFWVLGYG+GKF   +DEK
Sbjct: 452 TTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKFKPGIDEK 511

Query: 510 KLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVIFAEGVHKVGT 569
             NLKNPPLRNT +++PYGWTAIRFV DNPGVW FHCHIEPHLHMGMGV+FAEG++++G 
Sbjct: 512 TYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGLNRIGK 571

Query: 570 IPTKALACGGTAKVLIN 585
           +P +AL CG T + L+N
Sbjct: 572 VPDEALGCGLTKQFLMN 584

BLAST of Bhi02G001421 vs. TAIR10
Match: AT5G21100.1 (Plant L-ascorbate oxidase)

HSP 1 Score: 737.3 bits (1902), Expect = 7.6e-213
Identity = 344/547 (62.89%), Postives = 418/547 (76.42%), Query Frame = 0

Query: 43  WEVEYMFWSPDCVENLVMGINGQFPGPTIRANVGDTIIVELVNKLFTEGVVIHWHGILQR 102
           WEVEY +W PDC E +VM INGQFPGPTI A  GDT+I+ +VNKL TEGVVIHWHGI Q+
Sbjct: 28  WEVEYKYWWPDCKEGIVMAINGQFPGPTIDAVAGDTVIIHVVNKLSTEGVVIHWHGIRQK 87

Query: 103 ETPWADGTASISQCAINPGETFIYQFKADKPGTYFYHGHLGMQRSAGLYGSLIIDPEEGK 162
            TPWADG A ++QC INPGETF Y+F  DK GT+FYHGH GMQRS+GLYG LI+      
Sbjct: 88  GTPWADGAAGVTQCPINPGETFTYKFIVDKAGTHFYHGHYGMQRSSGLYGMLIV---RSP 147

Query: 163 KEPFHYDEEINLLLSDWWHKSAHKQEVGLSSSPMRWIGEPETILINGRGQISCSIAAKYN 222
           KE   YD E NLLLSDWWH+S H QE+ LSS PMRWIGEP+++LINGRGQ +CS AA +N
Sbjct: 148 KERLIYDGEFNLLLSDWWHQSIHAQELALSSRPMRWIGEPQSLLINGRGQFNCSQAAYFN 207

Query: 223 KNLEQ--CKLKGGEQCAPFILQVKPKKTYRIRIASTTSLSALNLAIGNHQLLVVEADGNY 282
           K  E+  C  K  +QCAP  L+V+P + YR+RIASTT+L++LNLA+  HQL+VVEADGNY
Sbjct: 208 KGGEKDVCTFKENDQCAPQTLRVEPNRVYRLRIASTTALASLNLAVQGHQLVVVEADGNY 267

Query: 283 VEPFVTSDIDIYSGESYSVLITTNQDPAQNYWVSVGVRGRLPKTPPALTLLNYLPNSVAK 342
           V PF  +DID+YSGE+YSVL+ TN  P++ YW+SVGVRGR PKTP ALT++NY+ ++   
Sbjct: 268 VAPFTVNDIDVYSGETYSVLLKTNALPSKKYWISVGVRGREPKTPQALTVINYV-DATES 327

Query: 343 LPVSPPPETPNWEDYARSKNFTYRILAAMGSPNPPIKFNRRIFLLNTQNRMNGGFTKWAI 402
            P  PPP TP W D  RSK+F+ +I AA G P PP K + ++ LLNTQN +   +TKW+I
Sbjct: 328 RPSHPPPVTPIWNDTDRSKSFSKKIFAAKGYPKPPEKSHDQLILLNTQN-LYEDYTKWSI 387

Query: 403 NNVSLSLPSTPYLGAIKIGLNNTFN-QNPPPETFSEDYDIFNPPPNPNTITDNGVYMFNL 462
           NNVSLS+P TPYLG+I+ GL + ++ ++P  +   ++YDI  PPPNPNT   +G+Y F  
Sbjct: 388 NNVSLSVPVTPYLGSIRYGLKSAYDLKSPAKKLIMDNYDIMKPPPNPNTTKGSGIYNFAF 447

Query: 463 GETVDVILQNANTLNDKHSEIHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNT 522
           G  VDVILQNAN L    SEIHPWH+HGHDFWVLGYGEGKF   +DEK  NLKNPPLRNT
Sbjct: 448 GIVVDVILQNANVLKGVISEIHPWHIHGHDFWVLGYGEGKFKPGIDEKTFNLKNPPLRNT 507

Query: 523 VVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVIFAEGVHKVG--TIPTKALACGG 582
           VV++P+GWTAIRFV DNPGVW FHCHIEPHLHMGMGV+F EGV ++G   IP +AL CG 
Sbjct: 508 VVLYPFGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFVEGVDRIGKMEIPDEALGCGL 567

Query: 583 TAKVLIN 585
           T K L+N
Sbjct: 568 TRKWLMN 569

BLAST of Bhi02G001421 vs. TAIR10
Match: AT4G39830.1 (Cupredoxin superfamily protein)

HSP 1 Score: 580.5 bits (1495), Expect = 1.2e-165
Identity = 277/549 (50.46%), Postives = 361/549 (65.76%), Query Frame = 0

Query: 39  RYFKWEVEYMFWSPDCVENLVMGINGQFPGPTIRANVGDTIIVELVNKLFTEGVVIHWHG 98
           R FKWEV+Y F SPDC E LV+ ING+FPGPTI+A  GDTI+VEL N   TE V +HWHG
Sbjct: 36  RRFKWEVKYEFKSPDCFEKLVITINGKFPGPTIKAQQGDTIVVELKNSFMTENVAVHWHG 95

Query: 99  ILQRETPWADGTASISQCAINPGETFIYQFKADKPGTYFYHGHLGMQRSAGLYGSLIIDP 158
           I Q  TPW DG   ++QC I PGE FIYQF  D+PGTY YH H GMQR +GL G + + P
Sbjct: 96  IRQIGTPWFDGVEGVTQCPILPGEVFIYQFVVDRPGTYMYHSHYGMQRESGLIGMIQVSP 155

Query: 159 EEGKKEPFHYDEEINLLLSDWWHKSAHKQEVGLSSSPMRWIGEPETILINGRGQISCSIA 218
              + EPF YD + N LL+DW+HKS  ++  GL+S P +W+GEP++++I GRG+ +CS  
Sbjct: 156 PATEPEPFTYDYDRNFLLTDWYHKSMSEKATGLASIPFKWVGEPQSLMIQGRGRFNCSNN 215

Query: 219 AKYNKNLEQ--CKLKGGEQCAPFILQVKPKKTYRIRIASTTSLSALNLAIGNHQLLVVEA 278
                +L    C +   + C+ FIL V P KTYR+RI S T+LSAL+  I  H L VVEA
Sbjct: 216 LTTPPSLVSGVCNVSNAD-CSRFILTVIPGKTYRLRIGSLTALSALSFQIEGHNLTVVEA 275

Query: 279 DGNYVEPFVTSDIDIYSGESYSVLITTNQDPAQNYWVSVGVRGRLPKTPPALTLLNYLPN 338
           DG+YVEPF   ++ +YSGE+YSVL+  +Q+P +NYW++  +  R   TPPA  +LNY PN
Sbjct: 276 DGHYVEPFTVKNLFVYSGETYSVLLKADQNPRRNYWITSSIVSRPATTPPATAVLNYYPN 335

Query: 339 SVAKLPVSPPPE--TPNWEDYARSKNFTYRILAAMGSPNP-PIKFNRRIFLLNTQNRMNG 398
              + P +       P W D       +  I A  G  +  P   ++ I LLNTQN +N 
Sbjct: 336 HPRRRPPTSESSNIVPEWNDTRSRLAQSLAIKARRGFIHALPENSDKVIVLLNTQNEVN- 395

Query: 399 GFTKWAINNVSLSLPSTPYLGAIKIGLNNTFN-QNPPPETF-SEDYDIFNPPPNPNTITD 458
           G+ +W++NNVS   P TPYL A+K  L N F+ +   PE + S +YDIF  P N N  T 
Sbjct: 396 GYRRWSVNNVSYHHPKTPYLIALKQNLTNAFDWRFTAPENYDSRNYDIFAKPLNANATTS 455

Query: 459 NGVYMFNLGETVDVILQNANTLNDKHSEIHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNL 518
           +G+Y      TVDVILQNANT+N  +SE HPWHLHGHDFWVLGYGEGKF+ + D K+ N 
Sbjct: 456 DGIYRLRFNSTVDVILQNANTMNANNSETHPWHLHGHDFWVLGYGEGKFNESEDPKRYNR 515

Query: 519 KNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVIFAEGVHKVGTIPTK 578
            +P  +NTV + P+GWTA+RF ADNPGVW+FHCHIE H  MGMG++F  G+ KV ++P+ 
Sbjct: 516 VDPIKKNTVAVQPFGWTALRFRADNPGVWSFHCHIESHFFMGMGIVFESGIDKVSSLPSS 575

Query: 579 ALACGGTAK 581
            + CG T +
Sbjct: 576 IMGCGQTKR 582

BLAST of Bhi02G001421 vs. TAIR10
Match: AT5G03260.1 (laccase 11)

HSP 1 Score: 261.5 bits (667), Expect = 1.2e-69
Identity = 175/566 (30.92%), Postives = 279/566 (49.29%), Query Frame = 0

Query: 17  IFLIFSMISGFGITLSEAVNNTRYFKWEVEYMFWSPDCVENLVMGINGQFPGPTIRANVG 76
           +FL   +++  G +  +A    + ++++V+    S  C    ++ +NG FPGPT+ A  G
Sbjct: 6   LFLFCYLLAFLGYSPVDAA--VKKYQFDVQVKNISRICNAKPIVTVNGMFPGPTVYAREG 65

Query: 77  DTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAINPGETFIYQFK-ADKPGT 136
           D +I+ + N +    + IHWHG+ Q    WADG A I+QC I  G++++Y F    + GT
Sbjct: 66  DRVIINVTNHV-QYNMSIHWHGLKQYRNGWADGPAYITQCPIQTGQSYLYDFNVTGQRGT 125

Query: 137 YFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYD-EEINLLLSDWWHKSAHKQEVGLSSS 196
            ++H H+   R A +YG+++I P  GK  PF    +E N++L +WW+K     +V  + +
Sbjct: 126 LWWHAHILWLR-ATVYGAIVILPAPGKPYPFPQPYQESNIILGEWWNK-----DVETAVN 185

Query: 197 PMRWIGEP----ETILINGRGQISCSIAAKYNKNLEQCKLKGGEQCAPFILQVKPKKTYR 256
               +G P    +   ING+       + K+                 F+++ +  KTY 
Sbjct: 186 QANQLGAPPPMSDAHTINGKPGPLFPCSEKHT----------------FVIEAEAGKTYL 245

Query: 257 IRIASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSVLITTNQDPAQN 316
           +RI +      L   I  H + VVE D  Y +PF T  I +  G++ +VL+ T++ P   
Sbjct: 246 LRIINAALNDELFFGIAGHNMTVVEIDAVYTKPFTTKAILLGPGQTTNVLVKTDRSP-NR 305

Query: 317 YWVSVGVRGRLP-----KTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSKNFTYRI 376
           Y+++       P     KT  A+     +PN+V  LP+ P    PN   +A   N     
Sbjct: 306 YFMAASPFMDAPVSVDNKTVTAILQYKGVPNTV--LPILPKLPLPNDTSFALDYNGK--- 365

Query: 377 LAAMGSPN----PPIKFNRRIF------LLNTQNRMNGGFTKWAINNVSLSLPSTPYLGA 436
           L ++ +PN     P+K +RR+F      +      +NG     +INN++  +P T  L A
Sbjct: 366 LKSLNTPNFPALVPLKVDRRLFYTIGLGINACPTCVNGTNLAASINNITFIMPKTALLKA 425

Query: 437 IKIGLNNTFN---QNPPPETFSEDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANT 496
               ++  F     + PP+ F  +Y       N  T T   +       T++++LQ+ N 
Sbjct: 426 HYSNISGVFRTDFPDRPPKAF--NYTGVPLTANLGTSTGTRLSRVKFNTTIELVLQDTNL 485

Query: 497 LNDKHSEIHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRF 556
           L     E HP+HLHG++F+V+G G G F    D  K NL +PP RNTV +   GW AIRF
Sbjct: 486 LT---VESHPFHLHGYNFFVVGTGVGNFDPKKDPAKFNLVDPPERNTVGVPTGGWAAIRF 535

Query: 557 VADNPGVWAFHCHIEPHLHMGMGVIF 559
            ADNPGVW  HCH+E H   G+ + F
Sbjct: 546 RADNPGVWFMHCHLEVHTMWGLKMAF 535

BLAST of Bhi02G001421 vs. TAIR10
Match: AT2G46570.1 (laccase 6)

HSP 1 Score: 245.0 bits (624), Expect = 1.2e-64
Identity = 177/589 (30.05%), Postives = 275/589 (46.69%), Query Frame = 0

Query: 1   MAKAANQPLFPLPFLVIFLIFSMISGFGITLSEAVNNTRYFKWEVEYMFWSPDCVENLVM 60
           M  +A   LF L FL+  L    I   G         TR+++++V+ +  +  C  N ++
Sbjct: 1   MTSSAVPSLFRLSFLLFTLQVMNIGRIGAA-------TRFYQFKVQTIRLTRLCQTNEIV 60

Query: 61  GINGQFPGPTIRANVGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAINP 120
            +N +FPGP I A   D I+++++N +      IHWHGI Q+ + W DG + I+QC I  
Sbjct: 61  TVNKKFPGPAISAQEDDRIVIKVIN-MTPYNTTIHWHGIKQKRSCWYDGPSYITQCPIQS 120

Query: 121 GETFIYQFK-ADKPGTYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYD-EEINLLLSD 180
           G++F Y FK A + GT+ +H H    R A +YG LI+ P+     PF     E  +LL +
Sbjct: 121 GQSFTYNFKVAQQKGTFLWHAHFSWLR-ATVYGPLIVYPKASVPYPFKKPFNEHTILLGE 180

Query: 181 WWHKSAHKQEVGLSSSPMRWIGEP---ETILINGRGQISCSIAAKYNKNLEQCKLKGGEQ 240
           +W K+     V L    +   G P   +   ING+          YN     C  K    
Sbjct: 181 YWLKNV----VELEQHVLESGGPPPPADAFTINGQP------GPNYN-----CSSKD--- 240

Query: 241 CAPFILQVKPKKTYRIRIASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGE 300
              + +Q+ P+K Y +R+ +          I NH+L +VE DG Y +P+ T  + +  G+
Sbjct: 241 --VYEIQIVPRKIYLLRLINAGINMETFFTIANHRLTIVEVDGEYTKPYTTERVMLVPGQ 300

Query: 301 SYSVLITTNQDPAQNYWVSVG-------VRGRLPKTPPALTLLNYLPNSVAKLPVSPPPE 360
           + ++L+T +Q   + Y +++G       V+ +          +  LPN+     V+ P +
Sbjct: 301 TMNILVTADQTVGR-YSMAMGPYESAKNVKFQNTSAIANFQYIGALPNN-----VTVPAK 360

Query: 361 TPNWEDYARSKNFTYRILAAMGSPNPPIKFNRRIFLL-----------NTQNRMNG---G 420
            P + D    K      L ++ + + P   +  +F+            N  N+  G   G
Sbjct: 361 LPIFNDNIAVKT-VMDGLRSLNAVDVPRNIDAHLFITIGLNVNKCNSENPNNKCQGPRKG 420

Query: 421 FTKWAINNVSLSLPSTPYLGAIKIGLNNTFNQNPPPETFSEDYDIFNPPPNP---NTITD 480
               ++NN+S   P    L A    L   F  +  P T  + YD  N  PN    +T   
Sbjct: 421 RLAASMNNISFIEPKVSILEAYYKQLEGYFTLD-FPTTPEKAYDFVNGAPNDIANDTQAA 480

Query: 481 NG--VYMFNLGETVDVILQNANTLNDKHSEIHPWHLHGHDFWVLGYGEGKFSAAVDEKKL 540
           NG    +F  G  + +I QN  TL    +E HP HLHGH F+V+GYG G +       K 
Sbjct: 481 NGTRAIVFEYGSRIQIIFQNTGTLT---TENHPIHLHGHSFYVIGYGTGNYDQ--QTAKF 540

Query: 541 NLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVIF 559
           NL++PP  NT+ +   GW AIRFVA+NPG+W  HCH + H   GM  +F
Sbjct: 541 NLEDPPYLNTIGVPVGGWAAIRFVANNPGLWLLHCHFDIHQTWGMSTMF 547

BLAST of Bhi02G001421 vs. TrEMBL
Match: tr|Q9M5B5|Q9M5B5_CUCME (Ascorbate oxidase AO1 OS=Cucumis melo OX=3656 GN=2C07 PE=2 SV=1)

HSP 1 Score: 1064.3 bits (2751), Expect = 9.9e-308
Identity = 492/588 (83.67%), Postives = 535/588 (90.99%), Query Frame = 0

Query: 3   KAANQPLFPLPFLVIFLIFSMISGFGITLSEAVNNTRYFKWEVEYMFWSPDCVENLVMGI 62
           K ANQPLFP+PF  IF IFS+  GFGITLS+A + TR+FKWEVEYMF SPDC ENLVMGI
Sbjct: 4   KVANQPLFPMPFPAIFFIFSIFFGFGITLSQAASQTRHFKWEVEYMFRSPDCKENLVMGI 63

Query: 63  NGQFPGPTIRANVGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAINPGE 122
           N QFPGPTIRANVGDT++VEL+NKL TE VVIHWHGILQ+ TPWADGTASISQCAINPGE
Sbjct: 64  NHQFPGPTIRANVGDTVVVELINKLSTEDVVIHWHGILQKGTPWADGTASISQCAINPGE 123

Query: 123 TFIYQFKADKPGTYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYDEEINLLLSDWWHK 182
           +F YQF  DKPGTYFYHGHLGMQR+AGLYGSLI+D EEGKKEPFHYDEEINLLLSDWWH+
Sbjct: 124 SFTYQFVVDKPGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEEINLLLSDWWHE 183

Query: 183 SAHKQEVGLSSSPMRWIGEPETILINGRGQISCSIAAKYNKNLEQCKLKGGEQCAPFILQ 242
           S  KQEVGLSSSPMRWIGEP++ILINGRGQ +CSI AKY KN+EQCKL G E+CAPFIL+
Sbjct: 184 SVLKQEVGLSSSPMRWIGEPQSILINGRGQSNCSIGAKYMKNMEQCKLSGSEECAPFILK 243

Query: 243 VKPKKTYRIRIASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSVLIT 302
           VKP KTYRIRI STTSLSALN AIGNH+LLVVEADGNYV+PF+TSDIDIYSGESYSVLI 
Sbjct: 244 VKPNKTYRIRIISTTSLSALNFAIGNHKLLVVEADGNYVQPFLTSDIDIYSGESYSVLIN 303

Query: 303 TNQDPAQNYWVSVGVRGRLPKTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSKNFT 362
           T+Q+P+QNYWVS+GVRGR PKTPP LTLLNYLPNS +KLP+SPPPETP W DY RSKNFT
Sbjct: 304 TDQNPSQNYWVSLGVRGREPKTPPGLTLLNYLPNSFSKLPISPPPETPKWNDYDRSKNFT 363

Query: 363 YRILAAMGSPNPPIKFNRRIFLLNTQNRMNGGFTKWAINNVSLSLPSTPYLGAIKIGLNN 422
           YRILAAMGSP PP+K  RRIFLLNTQN+MNGG+TKWAINNVSL+LPSTPYLGAIKIG+N+
Sbjct: 364 YRILAAMGSPKPPVKSTRRIFLLNTQNKMNGGYTKWAINNVSLTLPSTPYLGAIKIGIND 423

Query: 423 TFNQNPPPETFSEDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANTLNDKHSEIHP 482
           TF+QNPPPET+ E+YDI  PPPN NTIT NGVY F LGE VDV+LQNANTLNDKHSEIHP
Sbjct: 424 TFDQNPPPETYPENYDIHKPPPNGNTITGNGVYRFQLGEIVDVVLQNANTLNDKHSEIHP 483

Query: 483 WHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAF 542
           WHLHGHDFWVLGYGEGKFSAAVDE KLNLK+PPLRNTVVIFP+GWT +RFVADNPGVWAF
Sbjct: 484 WHLHGHDFWVLGYGEGKFSAAVDETKLNLKDPPLRNTVVIFPFGWTVLRFVADNPGVWAF 543

Query: 543 HCHIEPHLHMGMGVIFAEGVHKVGTIPTKALACGGTAKVLINNPTQKP 591
           HCHIEPHLHMGMGV+FAEGV KVG IPT A+ACGGTAK LI NPTQKP
Sbjct: 544 HCHIEPHLHMGMGVVFAEGVDKVGRIPTHAMACGGTAKALIQNPTQKP 591

BLAST of Bhi02G001421 vs. TrEMBL
Match: tr|A0A0A0LXB9|A0A0A0LXB9_CUCSA (L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=Csa_1G604040 PE=3 SV=1)

HSP 1 Score: 1009.6 bits (2609), Expect = 2.9e-291
Identity = 468/582 (80.41%), Postives = 517/582 (88.83%), Query Frame = 0

Query: 1   MAKAANQPLFPLPFLVIFLIFSMISGFGITLSEA-VNNTRYFKWEVEYMFWSPDCVENLV 60
           MAK A++P FP PFL  FL+ S+I GFGITLSEA     +++KW+VEYMFWSPDCVEN+V
Sbjct: 1   MAKVADKPFFPKPFL-SFLVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIV 60

Query: 61  MGINGQFPGPTIRANVGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAIN 120
           MGING+FPGPTIRAN GD ++VEL NKL TEGVVIHWHGILQR TPWADGTASISQCAIN
Sbjct: 61  MGINGEFPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAIN 120

Query: 121 PGETFIYQFKADKPGTYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYDEEINLLLSDW 180
           PGETF Y+F  DK GTYFYHGHLGMQRSAGLYGSLI+DP EG+ EPFHYDEEINLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDW 180

Query: 181 WHKSAHKQEVGLSSSPMRWIGEPETILINGRGQISCSIAAKYNKNLEQCKLKGGEQCAPF 240
           WH+S HKQEVGLSS PMRWIGEP++ILING+GQ  CSIAAKYN+ L+QC+L G E+CAPF
Sbjct: 181 WHQSVHKQEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPF 240

Query: 241 ILQVKPKKTYRIRIASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSV 300
           IL V+PKKTYRIRIASTT+L++LN AIGNH+LLVVEADGNYV+PFVTSDIDIYSGESYSV
Sbjct: 241 ILHVQPKKTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSV 300

Query: 301 LITTNQDPAQNYWVSVGVRGRLPKTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSK 360
           LITT+Q+P +NYWVS+GVR RLPKTPP LTLLNYLPNS +KLP+SPPPETP+WED+ RSK
Sbjct: 301 LITTDQNPLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSK 360

Query: 361 NFTYRILAAMGSPNPPIKFNRRIFLLNTQNRMNGGFTKWAINNVSLSLPSTPYLGAIKIG 420
           NFT+RI AAMGSP PP+++NRR+FLLNTQNR+N GF KWAINNVSL+LP TPYL A+K+ 
Sbjct: 361 NFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRIN-GFMKWAINNVSLALPPTPYLAAMKMR 420

Query: 421 LNNTFNQNPPPETFSEDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANTLNDKHSE 480
           LN  FNQNPPPETF  +YDI NPPPNP T T NGVY FN+GETVDVILQNAN LN   SE
Sbjct: 421 LNTAFNQNPPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSE 480

Query: 481 IHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
           IHPWHLHGHDFWVLGYGEGKF A  DEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540

Query: 541 WAFHCHIEPHLHMGMGVIFAEGVHKVGTIPTKALACGGTAKV 582
           WAFHCHIEPHLHMGMGV+FAEGVH VG IP KALACG TA V
Sbjct: 541 WAFHCHIEPHLHMGMGVVFAEGVHMVGMIPPKALACGSTALV 580

BLAST of Bhi02G001421 vs. TrEMBL
Match: tr|Q9M5B4|Q9M5B4_CUCME (Ascorbate oxidase AO4 OS=Cucumis melo OX=3656 GN=LOC103492479 PE=2 SV=1)

HSP 1 Score: 1008.1 bits (2605), Expect = 8.4e-291
Identity = 472/591 (79.86%), Postives = 518/591 (87.65%), Query Frame = 0

Query: 1   MAKAANQPLFPLPFLVIFLIFSMISGFGITLSEA-VNNTRYFKWEVEYMFWSPDCVENLV 60
           MAK A++P FP PFLV F + S+I GFGITLSEA     R++KWEVEYMFWSPDCVEN+V
Sbjct: 1   MAKVADKPFFPKPFLV-FFVLSIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIV 60

Query: 61  MGINGQFPGPTIRANVGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAIN 120
           MGINGQFPGPTIRAN GD ++VEL NKL TEGVVIHWHGILQR TPWADGTASISQCAIN
Sbjct: 61  MGINGQFPGPTIRANAGDMVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAIN 120

Query: 121 PGETFIYQFKADKPGTYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYDEEINLLLSDW 180
           PGETF Y+F  DK GTYFYHGHLGMQRSAGLYGSLI+DP+EG  EPFHYDEEINLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPQEGTSEPFHYDEEINLLLSDW 180

Query: 181 WHKSAHKQEVGLSSSPMRWIGEPETILINGRGQISCSIAAKYNKNLEQCKLKGGEQCAPF 240
           WH+S HKQEVGLSS PMRWIGEP++ILINGRGQ  CSIAAKY   L+QC+L G EQCAPF
Sbjct: 181 WHQSVHKQEVGLSSKPMRWIGEPQSILINGRGQFDCSIAAKYKNGLKQCELSGKEQCAPF 240

Query: 241 ILQVKPKKTYRIRIASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSV 300
           IL V PKK YRIRIASTT+L++LN AIGNH+LLVVEADGNYV+PF TSDIDIYSGESYSV
Sbjct: 241 ILHVHPKKIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFSTSDIDIYSGESYSV 300

Query: 301 LITTNQDPAQNYWVSVGVRGRLPKTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSK 360
           LITT+Q+P++NYWVS+GVR RLPKTPP LT+LNYLPNSV+KLP+SPPPETP+WED+ RSK
Sbjct: 301 LITTDQNPSENYWVSIGVRARLPKTPPGLTVLNYLPNSVSKLPISPPPETPDWEDFDRSK 360

Query: 361 NFTYRILAAMGSPNPPIKFNRRIFLLNTQNRMNGGFTKWAINNVSLSLPSTPYLGAIKIG 420
           NFT+RI AAMGSP PP+++NRR+FLLNTQNR+N GF KWAINNVSL+LP TPYL A+K  
Sbjct: 361 NFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRIN-GFMKWAINNVSLALPPTPYLAAMKFR 420

Query: 421 LNNTFNQNPPPETFSEDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANTLNDKHSE 480
           L+  FNQNPPPETF  DYDI NPP NP T T NGVY FN+GETVDVILQNAN LN   SE
Sbjct: 421 LSTAFNQNPPPETFPIDYDINNPPRNPETATGNGVYKFNMGETVDVILQNANMLNANTSE 480

Query: 481 IHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
           IHPWHLHGHDFWVLGYGEGKFSAA D +KLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFSAAEDGRKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540

Query: 541 WAFHCHIEPHLHMGMGVIFAEGVHKVGTIPTKALACGGTAKVLINNPTQKP 591
           WAFHCHIEPHLHMGMGVIFAEGVHKVG IP  A+ACG TA  +I N  +KP
Sbjct: 541 WAFHCHIEPHLHMGMGVIFAEGVHKVGMIPPNAMACGSTA--MIKNYPRKP 587

BLAST of Bhi02G001421 vs. TrEMBL
Match: tr|E7BBM8|E7BBM8_CUCSA (Ascorbate oxidase OS=Cucumis sativus OX=3659 GN=aso PE=2 SV=1)

HSP 1 Score: 1007.7 bits (2604), Expect = 1.1e-290
Identity = 467/582 (80.24%), Postives = 517/582 (88.83%), Query Frame = 0

Query: 1   MAKAANQPLFPLPFLVIFLIFSMISGFGITLSEA-VNNTRYFKWEVEYMFWSPDCVENLV 60
           MAK A++P FP PFL  FL+ S+I GFGITLSEA     +++KW+VEYMFWSPDCVEN+V
Sbjct: 1   MAKVADKPFFPKPFL-SFLVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIV 60

Query: 61  MGINGQFPGPTIRANVGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAIN 120
           MGING+FPGPTIRAN GD ++VEL NKL TEGVVIHWHGILQR TPWADGTASISQCAIN
Sbjct: 61  MGINGEFPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAIN 120

Query: 121 PGETFIYQFKADKPGTYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYDEEINLLLSDW 180
           PGETF Y+F  DK GTYFYHGHLGMQRSAGLYGSLI+DP EG+ EPFHYDEEINLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDW 180

Query: 181 WHKSAHKQEVGLSSSPMRWIGEPETILINGRGQISCSIAAKYNKNLEQCKLKGGEQCAPF 240
           WH+S HKQEVGLSS PMRWIGEP++ILING+GQ  CSIAAKYN+ L+QC+L G E+CAPF
Sbjct: 181 WHQSVHKQEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPF 240

Query: 241 ILQVKPKKTYRIRIASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSV 300
           IL V+PKKTYRIRIASTT+L++LN AIGNH+LLVVEADGNYV+PFVTSDIDIYSGESYSV
Sbjct: 241 ILHVQPKKTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSV 300

Query: 301 LITTNQDPAQNYWVSVGVRGRLPKTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSK 360
           LITT+Q+P +NYWVS+GVR RLPKTPP LTLLNYLPNS +KLP+SPPPETP+WED+ RSK
Sbjct: 301 LITTDQNPLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSK 360

Query: 361 NFTYRILAAMGSPNPPIKFNRRIFLLNTQNRMNGGFTKWAINNVSLSLPSTPYLGAIKIG 420
           NFT+RI AAMGSP PP+++NRR+FLLNTQNR+N GF KWAINNVSL+LP TPYL A+K+ 
Sbjct: 361 NFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRIN-GFMKWAINNVSLALPPTPYLAAMKMR 420

Query: 421 LNNTFNQNPPPETFSEDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANTLNDKHSE 480
           LN  FNQ+PPPETF  +YDI NPPPNP T T NGVY FN+GETVDVILQNAN LN   SE
Sbjct: 421 LNTAFNQSPPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSE 480

Query: 481 IHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
           IHPWHLHGHDFWVLGYGEGKF A  DEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540

Query: 541 WAFHCHIEPHLHMGMGVIFAEGVHKVGTIPTKALACGGTAKV 582
           WAFHCHIEPHLHMGMGV+FAEGVH VG IP KALACG TA V
Sbjct: 541 WAFHCHIEPHLHMGMGVVFAEGVHMVGMIPPKALACGSTALV 580

BLAST of Bhi02G001421 vs. TrEMBL
Match: tr|A0A0A0LZW2|A0A0A0LZW2_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G602540 PE=4 SV=1)

HSP 1 Score: 949.9 bits (2454), Expect = 2.7e-273
Identity = 437/539 (81.08%), Postives = 485/539 (89.98%), Query Frame = 0

Query: 3   KAANQPLFPLPFLVIFLIFSMISGFGITLSEAVNNTRYFKWEVEYMFWSPDCVENLVMGI 62
           K ANQPLFP+PF  +F + S+I GFGITLS+AV+ TR++ WEVEYMFWSPDC ENLVMGI
Sbjct: 4   KVANQPLFPMPFPAMFFLLSIIFGFGITLSDAVSQTRHYTWEVEYMFWSPDCKENLVMGI 63

Query: 63  NGQFPGPTIRANVGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAINPGE 122
           NGQFPGPTIRANVGDT++VE++NKL TE VVIHWHGILQ+ TPWADGTASISQCA NPGE
Sbjct: 64  NGQFPGPTIRANVGDTVVVEMINKLSTEDVVIHWHGILQKGTPWADGTASISQCATNPGE 123

Query: 123 TFIYQFKADKPGTYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYDEEINLLLSDWWHK 182
           +F YQF  DKPGTYFYHGHLGMQR+AGLYGSLI+DPEEG+KEPFHYDEEINLLLSDWWH+
Sbjct: 124 SFTYQFVVDKPGTYFYHGHLGMQRAAGLYGSLIVDPEEGEKEPFHYDEEINLLLSDWWHE 183

Query: 183 SAHKQEVGLSSSPMRWIGEPETILINGRGQISCSIAAKY--NKNLEQCKLKGGEQCAPFI 242
           S  KQE+GLSSSPMRWIGEP++ILINGRGQ +CSIAAKY   K +EQCKL G EQCAPFI
Sbjct: 184 SILKQEIGLSSSPMRWIGEPQSILINGRGQSNCSIAAKYMNMKKMEQCKLSGSEQCAPFI 243

Query: 243 LQVKPKKTYRIRIASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSVL 302
           L+VKP K YRIRI STTSLSALN AIG H+LLVVEADGNYV+PF+TSDIDIYSGESYSVL
Sbjct: 244 LEVKPNKIYRIRITSTTSLSALNFAIGKHKLLVVEADGNYVQPFLTSDIDIYSGESYSVL 303

Query: 303 ITTNQDPAQNYWVSVGVRGRLPKTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSKN 362
           I T+Q P++NYWVS+G+RGR PKTPP LT+LNYLPN V+KLP SPPPETP W DY RSKN
Sbjct: 304 INTDQKPSENYWVSLGIRGRDPKTPPGLTILNYLPNPVSKLPTSPPPETPKWNDYDRSKN 363

Query: 363 FTYRILAAMGSPNPPIKFNRRIFLLNTQNRMNGGFTKWAINNVSLSLPSTPYLGAIKIGL 422
           FT++ILAAMGSP PP+K  RRIFLLNTQN+MNGG+TKWAINNVSL+LPSTPYLGAIKIGL
Sbjct: 364 FTFQILAAMGSPKPPLKSTRRIFLLNTQNKMNGGYTKWAINNVSLALPSTPYLGAIKIGL 423

Query: 423 NNTFNQNPPPETFSEDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANTLNDKHSEI 482
           N+TF+QNPPPET+ EDYDI  PPPN NTIT NGVY F +GE VDV+LQNANTLNDKHSEI
Sbjct: 424 NHTFDQNPPPETYPEDYDINKPPPNRNTITGNGVYRFQIGEIVDVVLQNANTLNDKHSEI 483

Query: 483 HPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
           HPWHL GHDFWVLGYGEGKFS+A DE KLNLKNPPLRNTVVIFP+GWT +RFVADNPGV
Sbjct: 484 HPWHLPGHDFWVLGYGEGKFSSAADETKLNLKNPPLRNTVVIFPFGWTVLRFVADNPGV 542

BLAST of Bhi02G001421 vs. NCBI nr
Match: NP_001284440.1 (L-ascorbate oxidase-like [Cucumis melo] >AAF35910.1 ascorbate oxidase AO1 [Cucumis melo])

HSP 1 Score: 1064.3 bits (2751), Expect = 1.5e-307
Identity = 492/588 (83.67%), Postives = 535/588 (90.99%), Query Frame = 0

Query: 3   KAANQPLFPLPFLVIFLIFSMISGFGITLSEAVNNTRYFKWEVEYMFWSPDCVENLVMGI 62
           K ANQPLFP+PF  IF IFS+  GFGITLS+A + TR+FKWEVEYMF SPDC ENLVMGI
Sbjct: 4   KVANQPLFPMPFPAIFFIFSIFFGFGITLSQAASQTRHFKWEVEYMFRSPDCKENLVMGI 63

Query: 63  NGQFPGPTIRANVGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAINPGE 122
           N QFPGPTIRANVGDT++VEL+NKL TE VVIHWHGILQ+ TPWADGTASISQCAINPGE
Sbjct: 64  NHQFPGPTIRANVGDTVVVELINKLSTEDVVIHWHGILQKGTPWADGTASISQCAINPGE 123

Query: 123 TFIYQFKADKPGTYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYDEEINLLLSDWWHK 182
           +F YQF  DKPGTYFYHGHLGMQR+AGLYGSLI+D EEGKKEPFHYDEEINLLLSDWWH+
Sbjct: 124 SFTYQFVVDKPGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEEINLLLSDWWHE 183

Query: 183 SAHKQEVGLSSSPMRWIGEPETILINGRGQISCSIAAKYNKNLEQCKLKGGEQCAPFILQ 242
           S  KQEVGLSSSPMRWIGEP++ILINGRGQ +CSI AKY KN+EQCKL G E+CAPFIL+
Sbjct: 184 SVLKQEVGLSSSPMRWIGEPQSILINGRGQSNCSIGAKYMKNMEQCKLSGSEECAPFILK 243

Query: 243 VKPKKTYRIRIASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSVLIT 302
           VKP KTYRIRI STTSLSALN AIGNH+LLVVEADGNYV+PF+TSDIDIYSGESYSVLI 
Sbjct: 244 VKPNKTYRIRIISTTSLSALNFAIGNHKLLVVEADGNYVQPFLTSDIDIYSGESYSVLIN 303

Query: 303 TNQDPAQNYWVSVGVRGRLPKTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSKNFT 362
           T+Q+P+QNYWVS+GVRGR PKTPP LTLLNYLPNS +KLP+SPPPETP W DY RSKNFT
Sbjct: 304 TDQNPSQNYWVSLGVRGREPKTPPGLTLLNYLPNSFSKLPISPPPETPKWNDYDRSKNFT 363

Query: 363 YRILAAMGSPNPPIKFNRRIFLLNTQNRMNGGFTKWAINNVSLSLPSTPYLGAIKIGLNN 422
           YRILAAMGSP PP+K  RRIFLLNTQN+MNGG+TKWAINNVSL+LPSTPYLGAIKIG+N+
Sbjct: 364 YRILAAMGSPKPPVKSTRRIFLLNTQNKMNGGYTKWAINNVSLTLPSTPYLGAIKIGIND 423

Query: 423 TFNQNPPPETFSEDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANTLNDKHSEIHP 482
           TF+QNPPPET+ E+YDI  PPPN NTIT NGVY F LGE VDV+LQNANTLNDKHSEIHP
Sbjct: 424 TFDQNPPPETYPENYDIHKPPPNGNTITGNGVYRFQLGEIVDVVLQNANTLNDKHSEIHP 483

Query: 483 WHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAF 542
           WHLHGHDFWVLGYGEGKFSAAVDE KLNLK+PPLRNTVVIFP+GWT +RFVADNPGVWAF
Sbjct: 484 WHLHGHDFWVLGYGEGKFSAAVDETKLNLKDPPLRNTVVIFPFGWTVLRFVADNPGVWAF 543

Query: 543 HCHIEPHLHMGMGVIFAEGVHKVGTIPTKALACGGTAKVLINNPTQKP 591
           HCHIEPHLHMGMGV+FAEGV KVG IPT A+ACGGTAK LI NPTQKP
Sbjct: 544 HCHIEPHLHMGMGVVFAEGVDKVGRIPTHAMACGGTAKALIQNPTQKP 591

BLAST of Bhi02G001421 vs. NCBI nr
Match: XP_011660095.1 (PREDICTED: LOW QUALITY PROTEIN: L-ascorbate oxidase-like [Cucumis sativus])

HSP 1 Score: 1046.6 bits (2705), Expect = 3.2e-302
Identity = 482/590 (81.69%), Postives = 531/590 (90.00%), Query Frame = 0

Query: 3   KAANQPLFPLPFLVIFLIFSMISGFGITLSEAVNNTRYFKWEVEYMFWSPDCVENLVMGI 62
           K ANQPLFP+PF  +F + S+I GFGITLS+AV+ TR++ WEVEYMFWSPDC ENLVMGI
Sbjct: 4   KVANQPLFPMPFPAMFFLLSIIFGFGITLSDAVSQTRHYTWEVEYMFWSPDCKENLVMGI 63

Query: 63  NGQFPGPTIRANVGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAINPGE 122
           NGQFPGPTIRANVGDT++VE++NKL TE VVIHWHGILQ+ TPWADGTASISQCA NPGE
Sbjct: 64  NGQFPGPTIRANVGDTVVVEMINKLSTEDVVIHWHGILQKGTPWADGTASISQCATNPGE 123

Query: 123 TFIYQFKADKPGTYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYDEEINLLLSDWWHK 182
           +F YQF  DKPGTYFYHGHLGMQR+AGLYGSLI+DPEEG+KEPFHYDEEINLLLSDWWH+
Sbjct: 124 SFTYQFVVDKPGTYFYHGHLGMQRAAGLYGSLIVDPEEGEKEPFHYDEEINLLLSDWWHE 183

Query: 183 SAHKQEVGLSSSPMRWIGEPETILINGRGQISCSIAAKY--NKNLEQCKLKGGEQCAPFI 242
           S  KQE+GLSSSPMRWIGEP++ILINGRGQ +CSIAAKY   K +EQCKL G EQCAPFI
Sbjct: 184 SILKQEIGLSSSPMRWIGEPQSILINGRGQSNCSIAAKYMNMKKMEQCKLSGSEQCAPFI 243

Query: 243 LQVKPKKTYRIRIASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSVL 302
           L+VKP K YRIRI STTSLSALN AIG H+LLVVEADGNYV+PF+TSDIDIYSGESYSVL
Sbjct: 244 LEVKPNKIYRIRITSTTSLSALNFAIGKHKLLVVEADGNYVQPFLTSDIDIYSGESYSVL 303

Query: 303 ITTNQDPAQNYWVSVGVRGRLPKTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSKN 362
           I T+Q P++NYWVS+G+RGR PKTPP LT+LNYLPN V+KLP SPPPETP W DY RSKN
Sbjct: 304 INTDQKPSENYWVSLGIRGRDPKTPPGLTILNYLPNPVSKLPTSPPPETPKWNDYDRSKN 363

Query: 363 FTYRILAAMGSPNPPIKFNRRIFLLNTQNRMNGGFTKWAINNVSLSLPSTPYLGAIKIGL 422
           FT++ILAAMGSP PP+K  RRIFLLNTQN+MNGG+TKWAINNVSL+LPSTPYLGAIKIGL
Sbjct: 364 FTFQILAAMGSPKPPLKSTRRIFLLNTQNKMNGGYTKWAINNVSLALPSTPYLGAIKIGL 423

Query: 423 NNTFNQNPPPETFSEDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANTLNDKHSEI 482
           N+TF+QNPPPET+ EDYDI  PPPN NTIT NGVY F +GE VDV+LQNANTLNDKHSEI
Sbjct: 424 NHTFDQNPPPETYPEDYDINKPPPNRNTITGNGVYRFQIGEIVDVVLQNANTLNDKHSEI 483

Query: 483 HPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVW 542
           HPWHL GHDFWVLGYGEGKFS+A DE KLNLKNPPLRNTVVIFP+GWT +RFVADNPGV 
Sbjct: 484 HPWHLPGHDFWVLGYGEGKFSSAADETKLNLKNPPLRNTVVIFPFGWTVLRFVADNPGVX 543

Query: 543 AFHCHIEPHLHMGMGVIFAEGVHKVGTIPTKALACGGTAKVLINNPTQKP 591
           AFHCHIEPHLHMGMGVIFAEGVHKVG IPT A+ACGGTAK LI NPTQKP
Sbjct: 544 AFHCHIEPHLHMGMGVIFAEGVHKVGRIPTNAMACGGTAKALIRNPTQKP 593

BLAST of Bhi02G001421 vs. NCBI nr
Match: XP_004149015.1 (PREDICTED: L-ascorbate oxidase [Cucumis sativus] >P14133.1 RecName: Full=L-ascorbate oxidase; Short=ASO; Short=Ascorbase; Flags: Precursor >AAA33119.1 ascorbate oxidase precursor (EC 1.10.3.3) [Cucumis sativus] >KGN66418.1 L-ascorbate oxidase [Cucumis sativus])

HSP 1 Score: 1009.6 bits (2609), Expect = 4.4e-291
Identity = 468/582 (80.41%), Postives = 517/582 (88.83%), Query Frame = 0

Query: 1   MAKAANQPLFPLPFLVIFLIFSMISGFGITLSEA-VNNTRYFKWEVEYMFWSPDCVENLV 60
           MAK A++P FP PFL  FL+ S+I GFGITLSEA     +++KW+VEYMFWSPDCVEN+V
Sbjct: 1   MAKVADKPFFPKPFL-SFLVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIV 60

Query: 61  MGINGQFPGPTIRANVGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAIN 120
           MGING+FPGPTIRAN GD ++VEL NKL TEGVVIHWHGILQR TPWADGTASISQCAIN
Sbjct: 61  MGINGEFPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAIN 120

Query: 121 PGETFIYQFKADKPGTYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYDEEINLLLSDW 180
           PGETF Y+F  DK GTYFYHGHLGMQRSAGLYGSLI+DP EG+ EPFHYDEEINLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDW 180

Query: 181 WHKSAHKQEVGLSSSPMRWIGEPETILINGRGQISCSIAAKYNKNLEQCKLKGGEQCAPF 240
           WH+S HKQEVGLSS PMRWIGEP++ILING+GQ  CSIAAKYN+ L+QC+L G E+CAPF
Sbjct: 181 WHQSVHKQEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPF 240

Query: 241 ILQVKPKKTYRIRIASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSV 300
           IL V+PKKTYRIRIASTT+L++LN AIGNH+LLVVEADGNYV+PFVTSDIDIYSGESYSV
Sbjct: 241 ILHVQPKKTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSV 300

Query: 301 LITTNQDPAQNYWVSVGVRGRLPKTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSK 360
           LITT+Q+P +NYWVS+GVR RLPKTPP LTLLNYLPNS +KLP+SPPPETP+WED+ RSK
Sbjct: 301 LITTDQNPLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSK 360

Query: 361 NFTYRILAAMGSPNPPIKFNRRIFLLNTQNRMNGGFTKWAINNVSLSLPSTPYLGAIKIG 420
           NFT+RI AAMGSP PP+++NRR+FLLNTQNR+N GF KWAINNVSL+LP TPYL A+K+ 
Sbjct: 361 NFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRIN-GFMKWAINNVSLALPPTPYLAAMKMR 420

Query: 421 LNNTFNQNPPPETFSEDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANTLNDKHSE 480
           LN  FNQNPPPETF  +YDI NPPPNP T T NGVY FN+GETVDVILQNAN LN   SE
Sbjct: 421 LNTAFNQNPPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSE 480

Query: 481 IHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
           IHPWHLHGHDFWVLGYGEGKF A  DEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540

Query: 541 WAFHCHIEPHLHMGMGVIFAEGVHKVGTIPTKALACGGTAKV 582
           WAFHCHIEPHLHMGMGV+FAEGVH VG IP KALACG TA V
Sbjct: 541 WAFHCHIEPHLHMGMGVVFAEGVHMVGMIPPKALACGSTALV 580

BLAST of Bhi02G001421 vs. NCBI nr
Match: NP_001284441.1 (L-ascorbate oxidase [Cucumis melo] >AAF35911.2 ascorbate oxidase AO4 [Cucumis melo])

HSP 1 Score: 1008.1 bits (2605), Expect = 1.3e-290
Identity = 472/591 (79.86%), Postives = 518/591 (87.65%), Query Frame = 0

Query: 1   MAKAANQPLFPLPFLVIFLIFSMISGFGITLSEA-VNNTRYFKWEVEYMFWSPDCVENLV 60
           MAK A++P FP PFLV F + S+I GFGITLSEA     R++KWEVEYMFWSPDCVEN+V
Sbjct: 1   MAKVADKPFFPKPFLV-FFVLSIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIV 60

Query: 61  MGINGQFPGPTIRANVGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAIN 120
           MGINGQFPGPTIRAN GD ++VEL NKL TEGVVIHWHGILQR TPWADGTASISQCAIN
Sbjct: 61  MGINGQFPGPTIRANAGDMVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAIN 120

Query: 121 PGETFIYQFKADKPGTYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYDEEINLLLSDW 180
           PGETF Y+F  DK GTYFYHGHLGMQRSAGLYGSLI+DP+EG  EPFHYDEEINLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPQEGTSEPFHYDEEINLLLSDW 180

Query: 181 WHKSAHKQEVGLSSSPMRWIGEPETILINGRGQISCSIAAKYNKNLEQCKLKGGEQCAPF 240
           WH+S HKQEVGLSS PMRWIGEP++ILINGRGQ  CSIAAKY   L+QC+L G EQCAPF
Sbjct: 181 WHQSVHKQEVGLSSKPMRWIGEPQSILINGRGQFDCSIAAKYKNGLKQCELSGKEQCAPF 240

Query: 241 ILQVKPKKTYRIRIASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSV 300
           IL V PKK YRIRIASTT+L++LN AIGNH+LLVVEADGNYV+PF TSDIDIYSGESYSV
Sbjct: 241 ILHVHPKKIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFSTSDIDIYSGESYSV 300

Query: 301 LITTNQDPAQNYWVSVGVRGRLPKTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSK 360
           LITT+Q+P++NYWVS+GVR RLPKTPP LT+LNYLPNSV+KLP+SPPPETP+WED+ RSK
Sbjct: 301 LITTDQNPSENYWVSIGVRARLPKTPPGLTVLNYLPNSVSKLPISPPPETPDWEDFDRSK 360

Query: 361 NFTYRILAAMGSPNPPIKFNRRIFLLNTQNRMNGGFTKWAINNVSLSLPSTPYLGAIKIG 420
           NFT+RI AAMGSP PP+++NRR+FLLNTQNR+N GF KWAINNVSL+LP TPYL A+K  
Sbjct: 361 NFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRIN-GFMKWAINNVSLALPPTPYLAAMKFR 420

Query: 421 LNNTFNQNPPPETFSEDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANTLNDKHSE 480
           L+  FNQNPPPETF  DYDI NPP NP T T NGVY FN+GETVDVILQNAN LN   SE
Sbjct: 421 LSTAFNQNPPPETFPIDYDINNPPRNPETATGNGVYKFNMGETVDVILQNANMLNANTSE 480

Query: 481 IHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
           IHPWHLHGHDFWVLGYGEGKFSAA D +KLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFSAAEDGRKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540

Query: 541 WAFHCHIEPHLHMGMGVIFAEGVHKVGTIPTKALACGGTAKVLINNPTQKP 591
           WAFHCHIEPHLHMGMGVIFAEGVHKVG IP  A+ACG TA  +I N  +KP
Sbjct: 541 WAFHCHIEPHLHMGMGVIFAEGVHKVGMIPPNAMACGSTA--MIKNYPRKP 587

BLAST of Bhi02G001421 vs. NCBI nr
Match: CBY84386.1 (ascorbate oxidase [Cucumis sativus])

HSP 1 Score: 1007.7 bits (2604), Expect = 1.7e-290
Identity = 467/582 (80.24%), Postives = 517/582 (88.83%), Query Frame = 0

Query: 1   MAKAANQPLFPLPFLVIFLIFSMISGFGITLSEA-VNNTRYFKWEVEYMFWSPDCVENLV 60
           MAK A++P FP PFL  FL+ S+I GFGITLSEA     +++KW+VEYMFWSPDCVEN+V
Sbjct: 1   MAKVADKPFFPKPFL-SFLVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIV 60

Query: 61  MGINGQFPGPTIRANVGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAIN 120
           MGING+FPGPTIRAN GD ++VEL NKL TEGVVIHWHGILQR TPWADGTASISQCAIN
Sbjct: 61  MGINGEFPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAIN 120

Query: 121 PGETFIYQFKADKPGTYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYDEEINLLLSDW 180
           PGETF Y+F  DK GTYFYHGHLGMQRSAGLYGSLI+DP EG+ EPFHYDEEINLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDW 180

Query: 181 WHKSAHKQEVGLSSSPMRWIGEPETILINGRGQISCSIAAKYNKNLEQCKLKGGEQCAPF 240
           WH+S HKQEVGLSS PMRWIGEP++ILING+GQ  CSIAAKYN+ L+QC+L G E+CAPF
Sbjct: 181 WHQSVHKQEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPF 240

Query: 241 ILQVKPKKTYRIRIASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSV 300
           IL V+PKKTYRIRIASTT+L++LN AIGNH+LLVVEADGNYV+PFVTSDIDIYSGESYSV
Sbjct: 241 ILHVQPKKTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSV 300

Query: 301 LITTNQDPAQNYWVSVGVRGRLPKTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSK 360
           LITT+Q+P +NYWVS+GVR RLPKTPP LTLLNYLPNS +KLP+SPPPETP+WED+ RSK
Sbjct: 301 LITTDQNPLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSK 360

Query: 361 NFTYRILAAMGSPNPPIKFNRRIFLLNTQNRMNGGFTKWAINNVSLSLPSTPYLGAIKIG 420
           NFT+RI AAMGSP PP+++NRR+FLLNTQNR+N GF KWAINNVSL+LP TPYL A+K+ 
Sbjct: 361 NFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRIN-GFMKWAINNVSLALPPTPYLAAMKMR 420

Query: 421 LNNTFNQNPPPETFSEDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANTLNDKHSE 480
           LN  FNQ+PPPETF  +YDI NPPPNP T T NGVY FN+GETVDVILQNAN LN   SE
Sbjct: 421 LNTAFNQSPPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSE 480

Query: 481 IHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
           IHPWHLHGHDFWVLGYGEGKF A  DEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540

Query: 541 WAFHCHIEPHLHMGMGVIFAEGVHKVGTIPTKALACGGTAKV 582
           WAFHCHIEPHLHMGMGV+FAEGVH VG IP KALACG TA V
Sbjct: 541 WAFHCHIEPHLHMGMGVVFAEGVHMVGMIPPKALACGSTALV 580