Bhi02G000578 (gene) Wax gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Bhi02G000578
Type	gene
Organism	Benincasa hispida (Wax gourd)
Description	4-coumarate-CoA ligase
Location	chr2 : 15509336 .. 15512809 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACGGAATCGGGGTCAGCGATGACAATGTCGTTAGCGTTTGCAAAAGAGGGGTTCGAGATAAAATCAGGAGGTTGTGGGACGATAATGAGAAATACAGAGATGAAGATTATAAACCCTCAAACAGGAGCATCTCTTCCTAGGAATCAAGCTGGGGAGATATGTATTAAAAGTCTTCAGATAATGAAAGGTACACAATGCATTCTTTTATTTATTTAATTTCTAATTTAATGTGATATGATTGGAATTTATGTCTAAGAAAGATACTTCATTATTTTTATTTTTATTTTTTTATTTTTTATCTTATGTGTGTTGGAAAAATCAAATATTTTAACTTTGAGGTTGATATTAGTAGAATTTTTAGTACAATAAGGATAGGTGGGGAGAGGATTTTAAACTTCTTGTAGCAATCAACCCATTATGTTAGTATATTGTGATATCAATACAATTGATATTCCAATAATATTGATATCGATATCAATATTTTAATCATTGATTTCGAGTGGTCATTAATATTGATGTCAACCTTGTCATATTTTAATATTTTAATATAGGTTGAAACTTGACATCAACTTTATCTTAAATTCTAAATGTTTTTTTTCTTAATTATTTTTTATTTTACTTTTATATTTTAGTTCAATTCATTTGATCAATTTTCTTTCTGAATTTAAAATTTCATTGGATATACTAAAGTAAGTATAACTTAGTGGTAATTGACATGTATATTTTTTCTTAAGGTGTCAAATGTTTGAAATCTCCATATTCTTAATCGTTGTATTACAATATGTATATATATCATTGGATGCTACTTTAGTGTGTGTATATATATAGGCAAATAGATAAATAGGAAAATTTATGCAAACTTACCCTTGAGAATGCCAAATTTTATTGTACACCCACATTTTTTCGAATACATTAACTAATAAAAAAAGGATTTATCGAAACACTTAGGGGGCATCTTGGGAAAAATGTTGGTTATTATAAATTTAGGTTATTATAGTTGGATTATAATAGTTTATGTTAGCAGTGTAGATTATTTTAGGTTGAGTTAGAATAGTATGTGTTTGAGATATACACTATTTCAGTTTAAGAAGGAAATAGTAAACATTGTATCAAAGAATTAAAAAATGATAAATGTAACATAGTAAAAACTATAGTAAATATGGATTTAAAAATAGTGTTGATCGTTGCTAATTGAAAAATACAAAATAGTATCTACTATAATAAGAGGTGGTTAATCTTATGATAGTTCTTGCCGCAAACACATTCTTAATAAATAAACTAAATTTATTGGTGTAATAGTAGGAAACCTACCACTTTTAAGTTAGTGATGGATCAAATTAGAAGTTAATAGGTATAAGCGAGTTCCATTTAGATCAAGGGTGGTTTGCCATTTTCAAATTATTATTATTATTTGACTTTATTTAAACATGAATTTTGTTTGTGAATTTTCTTTTCACATTCACATCTTTAAAACATTTTGGTCTACTTTTTATTATAGTTCATGTTTTTTATTGATGTAAATATATACTATGAGTAAACTTGACGTATATGCTATTTAGTTAATAAGTTACATCACACATTTTATCTTTTAAATATAGAAATTAACCTGAAAATAATTTAAAAAAACTTGTGACGAAGATTTGGACAAATACTATGGTTGTATACTTCAACCTCTTAATTAATAATTGGTCAATTCATATATAAATTAAATATCTATGATACGCATATCAAAAGTACATCAATTAAATTAAAAATCGGTAGAAATAGAAATTAAAACTAGCATTTGGGTTAAAGTACTAGAAATATTTTGGTGAACAAAATAGGTTTTTATAATTAAAAAAAATTAGTTGAGAAATTAGTAGAATATGTTCATTAGTCCAATTTCATAATTCTTAATTTTTTTTAAAACTACGTTAACTCTTGTTTTTTTTAGTATAACAAGTAGAGTAGGAAAATGTTAACCTCTAACTCTAAGAAAGCAACAACATGTAAGTGATATTCTCGTTTGGATTATATTAATATTTTTTTACTACAATAATTAGGAACGATAAATACATATTAATTATCATTGAGTAAAACTCACTTTAATTTGTATTAACATTAATCCATTACCATTCACGGATGCATTGAAATAGTGAATATAAACAAAATATCTATTACCTTAATTGAAGTGAGTGTTCATTCCGAAACCCCTACAAAGGGGAAATCAAATTTTTACGAATAAAAGATTTAAATAATTAACATTTAACGGTGGAGTGTTGACAGCTGATGTCACTAATTATTGTCCTAATGAATGGTCGCCTGTCCGTTCCGAAATAAAAGAGCCAGGAGACGTCATTTAATTTGTTTTGATATGGAAGTACCAATTTGGGCCCAGCCCAGAGTTATTGGGCCTAATTGGGTTATTCCCAAGACTAAACCAAGCCCGATAAAATCGAGAGAGTCCATTACATTACTGTGTTGTGTGTTGCGTAAGTTTTGGTTGGTTCAACTGAACAGGATACCTCAACGATGAAGAGGCGACGAAGGGCATAATCGATAAAGACGGATGGCTGCACACTGGCGACGTTGGTTTTGTCGACGACGACGACGAGGTCTTTATCGTCGATCGTCTCAAGGAACTGATCAAATATAAAGGCTTCCAGGTCGCGCCGGCGGAGTTGGAAGCCCTGCTTATATCCCACGGCCACATCGCCGACGCCGCGGTTATACCGTACGTCAGCCTTCTCTGTTAGATTCCTTCACCCACTCTATTTTTAAATTAATTGTGCGTTTTCTTTTACACTAATTTGGTGACATTTCAGTATGAAAGATGAAGTTGCTGGAGAGGTTCCGGTTGCGTTCATTGCCCGATCCGATGGTACCAACATTACTGAGGATGAAATTAAGCAATACATCTCCAAACAGGTAAAACCTCCAATTTTGGCATTTATTTCAAGCTTTCGTGTCTGAAATCTTATTAGCTTTTGTTTATTGATGGAGATTTTAGAATCGCTTTCACGGTAAAATGAGGTAACTTTCCTTTTATGATATAACTCATCGTAGGCCCGTCTATAATTTCTTAACCTTTCACCATGTCTGTGCCTACCTTGACAAGTTGTGTCTATTTGATCAATACGCTTTACATAATTCTAACAACTGGTTGAACTTTTAATTCTGTGATTGATTTTAAGTTTTTATGCATAACATCGAAGGCTTAGAGATGGATGGCAAACATCAGAAATTTGTCTTTTTTATCTTTCAAAGTTCAAAAGCCTAATAAAACCAAACTTAATAGTTTAATAATCTTGATTGAAATCTGGGAGATCTAATGCATGCATGGATTGTAACTATTGAGGATGTGGGATGCAGGTTGTGTTTTATAAGAGGATTAATCGTGTCTTCTTCGTGGATTCCATTCCTAAAAGTCCTTCTGGCAAAATCTTGCGGAGACAACTTAGAGCTTTGCTCGCAGCCAGTATTCCTAATTAG

mRNA sequence

ATGACGGAATCGGGGTCAGCGATGACAATGTCGTTAGCGTTTGCAAAAGAGGGGTTCGAGATAAAATCAGGAGGTTGTGGGACGATAATGAGAAATACAGAGATGAAGATTATAAACCCTCAAACAGGAGCATCTCTTCCTAGGAATCAAGCTGGGGAGATATGTATTAAAAGTCTTCAGATAATGAAAGGATACCTCAACGATGAAGAGGCGACGAAGGGCATAATCGATAAAGACGGATGGCTGCACACTGGCGACGTTGGTTTTGTCGACGACGACGACGAGGTCTTTATCGTCGATCGTCTCAAGGAACTGATCAAATATAAAGGCTTCCAGGTCGCGCCGGCGGAGTTGGAAGCCCTGCTTATATCCCACGGCCACATCGCCGACGCCGCGGTTATACCTATGAAAGATGAAGTTGCTGGAGAGGTTCCGGTTGCGTTCATTGCCCGATCCGATGGTACCAACATTACTGAGGATGAAATTAAGCAATACATCTCCAAACAGGTTGTGTTTTATAAGAGGATTAATCGTGTCTTCTTCGTGGATTCCATTCCTAAAAGTCCTTCTGGCAAAATCTTGCGGAGACAACTTAGAGCTTTGCTCGCAGCCAGTATTCCTAATTAG

Coding sequence (CDS)

ATGACGGAATCGGGGTCAGCGATGACAATGTCGTTAGCGTTTGCAAAAGAGGGGTTCGAGATAAAATCAGGAGGTTGTGGGACGATAATGAGAAATACAGAGATGAAGATTATAAACCCTCAAACAGGAGCATCTCTTCCTAGGAATCAAGCTGGGGAGATATGTATTAAAAGTCTTCAGATAATGAAAGGATACCTCAACGATGAAGAGGCGACGAAGGGCATAATCGATAAAGACGGATGGCTGCACACTGGCGACGTTGGTTTTGTCGACGACGACGACGAGGTCTTTATCGTCGATCGTCTCAAGGAACTGATCAAATATAAAGGCTTCCAGGTCGCGCCGGCGGAGTTGGAAGCCCTGCTTATATCCCACGGCCACATCGCCGACGCCGCGGTTATACCTATGAAAGATGAAGTTGCTGGAGAGGTTCCGGTTGCGTTCATTGCCCGATCCGATGGTACCAACATTACTGAGGATGAAATTAAGCAATACATCTCCAAACAGGTTGTGTTTTATAAGAGGATTAATCGTGTCTTCTTCGTGGATTCCATTCCTAAAAGTCCTTCTGGCAAAATCTTGCGGAGACAACTTAGAGCTTTGCTCGCAGCCAGTATTCCTAATTAG

Protein sequence

MTESGSAMTMSLAFAKEGFEIKSGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIKSLQIMKGYLNDEEATKGIIDKDGWLHTGDVGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPMKDEVAGEVPVAFIARSDGTNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLAASIPN

Homology

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BLAST of Bhi02G000578 vs. Swiss-Prot
Match: sp|O24146|4CL2_TOBAC (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 8.8e-89
Identity = 156/208 (75.00%), Postives = 185/208 (88.94%), Query Frame = 0


Query: 1   MTESGSAMTMSLAFAKEGFEIKSGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIKSLQ 60
           MTE+G  + M LAFAKE FEIKSG CGT++RN EMKI++P+TG SLPRNQ+GEICI+  Q
Sbjct: 335 MTEAGPVLAMCLAFAKEPFEIKSGACGTVVRNAEMKIVDPKTGNSLPRNQSGEICIRGDQ 394

Query: 61  IMKGYLNDEEATKGIIDKDGWLHTGDVGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEA 120
           IMKGYLND EAT   IDK+GWL+TGD+G++DDDDE+FIVDRLKELIKYKGFQVAPAELEA
Sbjct: 395 IMKGYLNDPEATARTIDKEGWLYTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEA 454

Query: 121 LLISHGHIADAAVIPMKDEVAGEVPVAFIARSDGTNITEDEIKQYISKQVVFYKRINRVF 180
           LL++H +I+DAAV+PMKDE AGEVPVAF+ RS+G+ ITEDE+K +ISKQV+FYKRI RVF
Sbjct: 455 LLLNHPNISDAAVVPMKDEQAGEVPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVF 514

Query: 181 FVDSIPKSPSGKILRRQLRALLAASIPN 209
           FVD+IPKSPSGKILR+ LRA LAA +PN
Sbjct: 515 FVDAIPKSPSGKILRKDLRAKLAAGLPN 542

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BLAST of Bhi02G000578 vs. Swiss-Prot
Match: sp|P31686|4CL1_SOYBN (4-coumarate--CoA ligase 1 (Fragment) OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 2.6e-88
Identity = 157/208 (75.48%), Postives = 186/208 (89.42%), Query Frame = 0


Query: 1   MTESGSAMTMSLAFAKEGFEIKSGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIKSLQ 60
           MTE+G  +TMSLAFAKE  ++K G CGT++RN EMKI++P+TG SLPRNQ+GEICI+  Q
Sbjct: 85  MTEAGPVLTMSLAFAKEPIDVKPGACGTVVRNAEMKIVDPETGHSLPRNQSGEICIRGDQ 144

Query: 61  IMKGYLNDEEATKGIIDKDGWLHTGDVGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEA 120
           IMKGYLND EAT+  IDKDGWLHTGD+G++DDDDE+FIVDRLKELIKYKGFQVAPAELEA
Sbjct: 145 IMKGYLNDGEATERTIDKDGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEA 204

Query: 121 LLISHGHIADAAVIPMKDEVAGEVPVAFIARSDG-TNITEDEIKQYISKQVVFYKRINRV 180
           LL++H  I+DAAV+PMKDE AGEVPVAF+  S+G T+ TEDEIKQ+ISKQVVFYKRINRV
Sbjct: 205 LLLTHPKISDAAVVPMKDEAAGEVPVAFVVISNGYTDTTEDEIKQFISKQVVFYKRINRV 264

Query: 181 FFVDSIPKSPSGKILRRQLRALLAASIP 208
           FF+D+IPKSPSGKILR+ LRA +AAS+P
Sbjct: 265 FFIDAIPKSPSGKILRKDLRAKIAASVP 292

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BLAST of Bhi02G000578 vs. Swiss-Prot
Match: sp|O24145|4CL1_TOBAC (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 7.5e-88
Identity = 152/208 (73.08%), Postives = 183/208 (87.98%), Query Frame = 0


Query: 1   MTESGSAMTMSLAFAKEGFEIKSGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIKSLQ 60
           MTE+G  + M LAFAKE F+IKSG CGT++RN EMKI++P TG SLPRNQ GEICI+  Q
Sbjct: 340 MTEAGPVLAMCLAFAKEPFDIKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQ 399

Query: 61  IMKGYLNDEEATKGIIDKDGWLHTGDVGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEA 120
           IMKGYLND EAT   IDK+GWLHTGD+GF+D+DDE+FIVDRLKELIKYKGFQVAPAE+EA
Sbjct: 400 IMKGYLNDPEATTRTIDKEGWLHTGDIGFIDEDDELFIVDRLKELIKYKGFQVAPAEIEA 459

Query: 121 LLISHGHIADAAVIPMKDEVAGEVPVAFIARSDGTNITEDEIKQYISKQVVFYKRINRVF 180
           LL++H +I+DAAV+PMKDE AGEVPVAF+ RS+G+ ITEDE+K +ISKQV+FYKR+ RVF
Sbjct: 460 LLLNHPNISDAAVVPMKDEQAGEVPVAFVVRSNGSAITEDEVKDFISKQVIFYKRVKRVF 519

Query: 181 FVDSIPKSPSGKILRRQLRALLAASIPN 209
           FV+++PKSPSGKILR+ LRA LAA +PN
Sbjct: 520 FVETVPKSPSGKILRKDLRARLAAGVPN 547

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BLAST of Bhi02G000578 vs. Swiss-Prot
Match: sp|O24540|4CL_VANPL (4-coumarate--CoA ligase OS=Vanilla planifolia OX=51239 GN=4CL PE=3 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 1.7e-87
Identity = 154/205 (75.12%), Postives = 183/205 (89.27%), Query Frame = 0


Query: 1   MTESGSAMTMSLAFAKEGFEIKSGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIKSLQ 60
           MTE+G  + M LAFAKE F+IKSG CGT++RN EMKI++P+TG+SLPRN  GEICI+  Q
Sbjct: 344 MTEAGPVLAMCLAFAKEPFDIKSGACGTVVRNAEMKIVDPETGSSLPRNHPGEICIRGDQ 403

Query: 61  IMKGYLNDEEATKGIIDKDGWLHTGDVGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEA 120
           IMKGYLND EAT   IDK+GWLHTGD+G++DDDDE+FIVDRLKELIKYKGFQVAPAELEA
Sbjct: 404 IMKGYLNDPEATARTIDKEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEA 463

Query: 121 LLISHGHIADAAVIPMKDEVAGEVPVAFIARSDGTNITEDEIKQYISKQVVFYKRINRVF 180
           LL++H  I+DAAV+PMKDE AGEVPVAF+ +S+G NITEDEIKQ+ISKQV+FYKRINRVF
Sbjct: 464 LLLTHPCISDAAVVPMKDEAAGEVPVAFVVKSNGHNITEDEIKQFISKQVIFYKRINRVF 523

Query: 181 FVDSIPKSPSGKILRRQLRALLAAS 206
           FV++IPK+PSGKILR+ LRA LAA+
Sbjct: 524 FVEAIPKAPSGKILRKDLRARLAAA 548

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BLAST of Bhi02G000578 vs. Swiss-Prot
Match: sp|P31684|4CL1_SOLTU (4-coumarate--CoA ligase 1 OS=Solanum tuberosum OX=4113 GN=4CL1 PE=3 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 1.1e-86
Identity = 154/208 (74.04%), Postives = 180/208 (86.54%), Query Frame = 0


Query: 1   MTESGSAMTMSLAFAKEGFEIKSGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIKSLQ 60
           MTE+G  + M LAFAKE F+IKSG CGT++RN EMKI++P TG SLPRNQ GEICI+  Q
Sbjct: 338 MTEAGPVLAMCLAFAKEPFDIKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQ 397

Query: 61  IMKGYLNDEEATKGIIDKDGWLHTGDVGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEA 120
           IMKGYLND EAT   I+K+GWLHTGD+GF+DDDDE+FIVDRLKELIKYKGFQVAPAELEA
Sbjct: 398 IMKGYLNDPEATARTIEKEGWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEA 457

Query: 121 LLISHGHIADAAVIPMKDEVAGEVPVAFIARSDGTNITEDEIKQYISKQVVFYKRINRVF 180
           LLI+H  I+DAAV+PM DE AGEVPVAF+ RS+G+ ITEDE+K +ISKQV+FYKRI RVF
Sbjct: 458 LLINHPDISDAAVVPMIDEQAGEVPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVF 517

Query: 181 FVDSIPKSPSGKILRRQLRALLAASIPN 209
           FV+++PKSPSGKILR+ LRA LAA I N
Sbjct: 518 FVETVPKSPSGKILRKDLRARLAAGISN 545

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BLAST of Bhi02G000578 vs. TAIR10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2)

HSP 1 Score: 310.5 bits (794), Expect = 8.1e-85
Identity = 151/208 (72.60%), Postives = 174/208 (83.65%), Query Frame = 0


Query: 1   MTESGSAMTMSLAFAKEGFEIKSGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIKSLQ 60
           MTE+G  + MSL FAKE F +KSG CGT++RN EMKI++P TG SLPRN+ GEICI+  Q
Sbjct: 349 MTEAGPVLAMSLGFAKEPFPVKSGACGTVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQ 408

Query: 61  IMKGYLNDEEATKGIIDKDGWLHTGDVGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEA 120
           IMKGYLND  AT   IDKDGWLHTGDVGF+DDDDE+FIVDRLKELIKYKGFQVAPAELE+
Sbjct: 409 IMKGYLNDPLATASTIDKDGWLHTGDVGFIDDDDELFIVDRLKELIKYKGFQVAPAELES 468

Query: 121 LLISHGHIADAAVIPMKDEVAGEVPVAFIARSDGTNITEDEIKQYISKQVVFYKRINRVF 180
           LLI H  I D AV+ MK+E AGEVPVAF+ RS  +NI+EDEIKQ++SKQVVFYKRIN+VF
Sbjct: 469 LLIGHPEINDVAVVAMKEEDAGEVPVAFVVRSKDSNISEDEIKQFVSKQVVFYKRINKVF 528

Query: 181 FVDSIPKSPSGKILRRQLRALLAASIPN 209
           F DSIPK+PSGKILR+ LRA LA  + N
Sbjct: 529 FTDSIPKAPSGKILRKDLRARLANGLMN 556

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BLAST of Bhi02G000578 vs. TAIR10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1)

HSP 1 Score: 298.5 bits (763), Expect = 3.2e-81
Identity = 142/203 (69.95%), Postives = 169/203 (83.25%), Query Frame = 0


Query: 1   MTESGSAMTMSLAFAKEGFEIKSGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIKSLQ 60
           MTE+G  + MSL FAKE F +KSG CGT++RN EMKI++P TG SL RNQ GEICI+  Q
Sbjct: 356 MTEAGPVLAMSLGFAKEPFPVKSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQ 415

Query: 61  IMKGYLNDEEATKGIIDKDGWLHTGDVGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEA 120
           IMKGYLN+  AT   IDKDGWLHTGD+G +DDDDE+FIVDRLKELIKYKGFQVAPAELEA
Sbjct: 416 IMKGYLNNPAATAETIDKDGWLHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEA 475

Query: 121 LLISHGHIADAAVIPMKDEVAGEVPVAFIARSDGTNITEDEIKQYISKQVVFYKRINRVF 180
           LLI H  I D AV+ MK+E AGEVPVAF+ +S  + ++ED++KQ++SKQVVFYKRIN+VF
Sbjct: 476 LLIGHPDITDVAVVAMKEEAAGEVPVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVF 535

Query: 181 FVDSIPKSPSGKILRRQLRALLA 204
           F +SIPK+PSGKILR+ LRA LA
Sbjct: 536 FTESIPKAPSGKILRKDLRAKLA 558

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BLAST of Bhi02G000578 vs. TAIR10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5)

HSP 1 Score: 292.0 bits (746), Expect = 3.0e-79
Identity = 141/202 (69.80%), Postives = 171/202 (84.65%), Query Frame = 0


Query: 1   MTESGSAMTMSLAFAKEGFEIKSGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIKSLQ 60
           MTESG+ +  SLAFAK  F+ KSG CGT++RN EMK+++ +TG SLPRN++GEIC++  Q
Sbjct: 364 MTESGT-VAKSLAFAKNPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQ 423

Query: 61  IMKGYLNDEEATKGIIDKDGWLHTGDVGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEA 120
           +MKGYLND EAT   IDKDGWLHTGD+GFVDDDDE+FIVDRLKELIK+KG+QVAPAELEA
Sbjct: 424 LMKGYLNDPEATARTIDKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEA 483

Query: 121 LLISHGHIADAAVIPMKDEVAGEVPVAFIARSDGTNITEDEIKQYISKQVVFYKRINRVF 180
           LLISH  I DAAV+ MKDEVA EVPVAF+ARS G+ +TED++K Y++KQVV YKRI  VF
Sbjct: 484 LLISHPSIDDAAVVAMKDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVF 543

Query: 181 FVDSIPKSPSGKILRRQLRALL 203
           F++ IPK+ SGKILR+ LRA L
Sbjct: 544 FIEVIPKAVSGKILRKDLRAKL 564

[Close]

BLAST of Bhi02G000578 vs. TAIR10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3)

HSP 1 Score: 285.4 bits (729), Expect = 2.8e-77
Identity = 134/202 (66.34%), Postives = 170/202 (84.16%), Query Frame = 0


Query: 1   MTESGSAMTMSLAFAKEGFEIKSGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIKSLQ 60
           MTE+G  ++MSL FAKE    KSG CGT++RN E+K+++ +T  SL  NQ GEICI+  Q
Sbjct: 359 MTEAGPVLSMSLGFAKEPIPTKSGSCGTVVRNAELKVVHLETRLSLGYNQPGEICIRGQQ 418

Query: 61  IMKGYLNDEEATKGIIDKDGWLHTGDVGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEA 120
           IMK YLND EAT   ID++GWLHTGD+G+VD+DDE+FIVDRLKE+IK+KGFQV PAELE+
Sbjct: 419 IMKEYLNDPEATSATIDEEGWLHTGDIGYVDEDDEIFIVDRLKEVIKFKGFQVPPAELES 478

Query: 121 LLISHGHIADAAVIPMKDEVAGEVPVAFIARSDGTNITEDEIKQYISKQVVFYKRINRVF 180
           LLI+H  IADAAV+P  DEVAGEVPVAF+ RS+G +ITE+++K+Y++KQVVFYKR+++VF
Sbjct: 479 LLINHHSIADAAVVPQNDEVAGEVPVAFVVRSNGNDITEEDVKEYVAKQVVFYKRLHKVF 538

Query: 181 FVDSIPKSPSGKILRRQLRALL 203
           FV SIPKSPSGKILR+ L+A L
Sbjct: 539 FVASIPKSPSGKILRKDLKAKL 560

[Close]

BLAST of Bhi02G000578 vs. TAIR10
Match: AT4G05160.1 (AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 209.1 bits (531), Expect = 2.5e-54
Identity = 98/176 (55.68%), Postives = 132/176 (75.00%), Query Frame = 0


Query: 23  SGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIKSLQIMKGYLNDEEATKGIIDKDGWL 82
           SG  G +    E +I++ +TG S P NQ GEI ++   +MKGYLN+ +ATK  IDK  W+
Sbjct: 361 SGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKSWV 420

Query: 83  HTGDVGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPMKDEVAG 142
           HTGD+G+ ++D  +++VDR+KELIKYKGFQVAPAELE LL+SH  I DA VIP  DE AG
Sbjct: 421 HTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEEAG 480

Query: 143 EVPVAFIARSDGTNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQL 199
           EVP+AF+ RS  ++ITE +I+++I+KQV  YKR+ RV F+  +PKS +GKILRR+L
Sbjct: 481 EVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRREL 536

[Close]

BLAST of Bhi02G000578 vs. TrEMBL
Match: tr|A0A1S3BWP0|A0A1S3BWP0_CUCME (4-coumarate--CoA ligase 1-like OS=Cucumis melo OX=3656 GN=LOC103494274 PE=4 SV=1)

HSP 1 Score: 372.9 bits (956), Expect = 4.8e-100
Identity = 185/206 (89.81%), Postives = 195/206 (94.66%), Query Frame = 0


Query: 1   MTESGSAMTMSLAFAKEGFEIKSGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIKSLQ 60
           MTE+GS MTMSLAFAKEGF IKSGGCGTIMRN+EMKI+ PQTG SLPRN+ GEICI+S Q
Sbjct: 345 MTEAGSVMTMSLAFAKEGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQ 404

Query: 61  IMKGYLNDEEATKGIIDKDGWLHTGDVGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEA 120
           +MKGYLNDEEATK IIDKDGWLHTGD+G+VDDDDE+FIVDRLKELIKYKGFQVAPAELEA
Sbjct: 405 MMKGYLNDEEATKAIIDKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEA 464

Query: 121 LLISHGHIADAAVIPMKDEVAGEVPVAFIARSDGTNITEDEIKQYISKQVVFYKRINRVF 180
           LLISHGHIADAAVIPM DEVAGEVPV FI R DG+NITEDEIKQYISKQVVFYKRINRVF
Sbjct: 465 LLISHGHIADAAVIPMNDEVAGEVPVTFIVRFDGSNITEDEIKQYISKQVVFYKRINRVF 524

Query: 181 FVDSIPKSPSGKILRRQLRALLAASI 207
           FVDSIPKSPSGKILRRQLRALLAASI
Sbjct: 525 FVDSIPKSPSGKILRRQLRALLAASI 550

[Close]

BLAST of Bhi02G000578 vs. TrEMBL
Match: tr|A0A0A0LWT6|A0A0A0LWT6_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G423020 PE=4 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 2.6e-98
Identity = 183/206 (88.83%), Postives = 194/206 (94.17%), Query Frame = 0


Query: 1   MTESGSAMTMSLAFAKEGFEIKSGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIKSLQ 60
           MTE+GS MTMSLAF KEGF IKSGGCGTIMRN+EMKI+N QTG SLPRNQ GEICI+S Q
Sbjct: 340 MTEAGSVMTMSLAFVKEGFGIKSGGCGTIMRNSEMKILNLQTGESLPRNQTGEICIRSSQ 399

Query: 61  IMKGYLNDEEATKGIIDKDGWLHTGDVGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEA 120
           +MKGYLNDE+ATK IID+DGWLHTGD+GFVDDDDE+FIVDRLKELIKYKGFQVAPAELEA
Sbjct: 400 MMKGYLNDEDATKAIIDEDGWLHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEA 459

Query: 121 LLISHGHIADAAVIPMKDEVAGEVPVAFIARSDGTNITEDEIKQYISKQVVFYKRINRVF 180
           LLISH HIADAAVIPM DEVAGEVPVAFI R DG+NITEDEIKQ+ISKQVVFYKRINRVF
Sbjct: 460 LLISHAHIADAAVIPMNDEVAGEVPVAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVF 519

Query: 181 FVDSIPKSPSGKILRRQLRALLAASI 207
           FVDSIPKSPSGKILRRQLRALLAASI
Sbjct: 520 FVDSIPKSPSGKILRRQLRALLAASI 545

[Close]

BLAST of Bhi02G000578 vs. TrEMBL
Match: tr|A0A068FPP7|A0A068FPP7_GARMA (4-coumarate coenzymeA ligase (Fragment) OS=Garcinia mangostana OX=58228 GN=4CL PE=2 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 7.7e-90
Identity = 161/208 (77.40%), Postives = 186/208 (89.42%), Query Frame = 0


Query: 1   MTESGSAMTMSLAFAKEGFEIKSGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIKSLQ 60
           MTE+G  + M LAFAKE FEIKSG CGT++RN EMKI++P+TG SLPRNQ GEICI+  Q
Sbjct: 139 MTEAGPVLAMCLAFAKEPFEIKSGACGTVVRNAEMKIVDPETGFSLPRNQPGEICIRGNQ 198

Query: 61  IMKGYLNDEEATKGIIDKDGWLHTGDVGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEA 120
           IMKGY+ND EAT+  IDK GWLHTGD+G++DDDDE+FIVDRLKELIKYKGFQVAPAELEA
Sbjct: 199 IMKGYVNDPEATERTIDKQGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEA 258

Query: 121 LLISHGHIADAAVIPMKDEVAGEVPVAFIARSDGTNITEDEIKQYISKQVVFYKRINRVF 180
           +LI+H HI+DAAVIPMKDE AGEVPVAF+ RS+G+NITEDEIKQYISKQV+FYKRINRVF
Sbjct: 259 MLIAHPHISDAAVIPMKDEAAGEVPVAFVVRSNGSNITEDEIKQYISKQVIFYKRINRVF 318

Query: 181 FVDSIPKSPSGKILRRQLRALLAASIPN 209
           F D+IPK+PSGKILR+ LRA LAA +PN
Sbjct: 319 FTDTIPKAPSGKILRKDLRARLAAGVPN 346

[Close]

BLAST of Bhi02G000578 vs. TrEMBL
Match: tr|I6PD12|I6PD12_9MAGN (4-coumarate-CoA ligase OS=Cinnamomum osmophloeum OX=258907 GN=4Cl1 PE=2 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 1.7e-89
Identity = 160/208 (76.92%), Postives = 190/208 (91.35%), Query Frame = 0


Query: 1   MTESGSAMTMSLAFAKEGFEIKSGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIKSLQ 60
           MTE+G  ++M LAFAKE FEIKSG CGT++RN E+KI++P+TGASLPRNQAGEICI+  Q
Sbjct: 332 MTEAGPVLSMCLAFAKEPFEIKSGSCGTVVRNAELKIVDPETGASLPRNQAGEICIRGSQ 391

Query: 61  IMKGYLNDEEATKGIIDKDGWLHTGDVGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEA 120
           IMKGYLND EATK  IDK+GWLHTGD+GFVDDDDE+FIVDRLKELIKYKGFQVAPAELEA
Sbjct: 392 IMKGYLNDPEATKMTIDKEGWLHTGDIGFVDDDDEIFIVDRLKELIKYKGFQVAPAELEA 451

Query: 121 LLISHGHIADAAVIPMKDEVAGEVPVAFIARSDGTNITEDEIKQYISKQVVFYKRINRVF 180
           +LI+H ++ADAAV+PMKD +AGEVPVAF+ +S+ +NITE+EIKQ+++KQVVFYKRINRVF
Sbjct: 452 MLITHPNVADAAVVPMKDVLAGEVPVAFVVKSNSSNITEEEIKQFVAKQVVFYKRINRVF 511

Query: 181 FVDSIPKSPSGKILRRQLRALLAASIPN 209
           FVDSIPK+PSGKILR+ LRA LAA +PN
Sbjct: 512 FVDSIPKAPSGKILRKDLRARLAAGMPN 539

[Close]

BLAST of Bhi02G000578 vs. TrEMBL
Match: tr|A0A1R3G3Z5|A0A1R3G3Z5_COCAP (AMP-dependent synthetase/ligase OS=Corchorus capsularis OX=210143 GN=CCACVL1_29084 PE=4 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 2.9e-89
Identity = 160/208 (76.92%), Postives = 187/208 (89.90%), Query Frame = 0


Query: 1   MTESGSAMTMSLAFAKEGFEIKSGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIKSLQ 60
           MTE+G  + M+LAFAKE FE KSG CGT++RN EMKI+NP+TGASLPRNQ+GEICI+  Q
Sbjct: 337 MTEAGPVLAMNLAFAKEAFETKSGACGTVVRNAEMKIVNPETGASLPRNQSGEICIRGSQ 396

Query: 61  IMKGYLNDEEATKGIIDKDGWLHTGDVGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEA 120
           IMKGYLND EAT+  IDKDGWLHTGD+G++D+DDE+FIVDRLKELIKYKGFQVAPAELEA
Sbjct: 397 IMKGYLNDPEATERTIDKDGWLHTGDIGYIDEDDELFIVDRLKELIKYKGFQVAPAELEA 456

Query: 121 LLISHGHIADAAVIPMKDEVAGEVPVAFIARSDGTNITEDEIKQYISKQVVFYKRINRVF 180
           +LI+H +I+DAAV+PMKDE AGEVPVAF+ RS+G+ ITEDEIKQ+ISKQVVFYKR+ RVF
Sbjct: 457 MLIAHPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKITEDEIKQFISKQVVFYKRLARVF 516

Query: 181 FVDSIPKSPSGKILRRQLRALLAASIPN 209
           F DSIPK+PSGKILR+ LRA LAA IPN
Sbjct: 517 FTDSIPKAPSGKILRKDLRARLAAEIPN 544

[Close]

BLAST of Bhi02G000578 vs. NCBI nr
Match: XP_008453615.1 (PREDICTED: 4-coumarate--CoA ligase 1-like [Cucumis melo])

HSP 1 Score: 372.9 bits (956), Expect = 7.3e-100
Identity = 185/206 (89.81%), Postives = 195/206 (94.66%), Query Frame = 0


Query: 1   MTESGSAMTMSLAFAKEGFEIKSGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIKSLQ 60
           MTE+GS MTMSLAFAKEGF IKSGGCGTIMRN+EMKI+ PQTG SLPRN+ GEICI+S Q
Sbjct: 345 MTEAGSVMTMSLAFAKEGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQ 404

Query: 61  IMKGYLNDEEATKGIIDKDGWLHTGDVGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEA 120
           +MKGYLNDEEATK IIDKDGWLHTGD+G+VDDDDE+FIVDRLKELIKYKGFQVAPAELEA
Sbjct: 405 MMKGYLNDEEATKAIIDKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEA 464

Query: 121 LLISHGHIADAAVIPMKDEVAGEVPVAFIARSDGTNITEDEIKQYISKQVVFYKRINRVF 180
           LLISHGHIADAAVIPM DEVAGEVPV FI R DG+NITEDEIKQYISKQVVFYKRINRVF
Sbjct: 465 LLISHGHIADAAVIPMNDEVAGEVPVTFIVRFDGSNITEDEIKQYISKQVVFYKRINRVF 524

Query: 181 FVDSIPKSPSGKILRRQLRALLAASI 207
           FVDSIPKSPSGKILRRQLRALLAASI
Sbjct: 525 FVDSIPKSPSGKILRRQLRALLAASI 550

[Close]

BLAST of Bhi02G000578 vs. NCBI nr
Match: XP_004146311.1 (PREDICTED: 4-coumarate--CoA ligase 1-like [Cucumis sativus] >KGN65444.1 hypothetical protein Csa_1G423020 [Cucumis sativus])

HSP 1 Score: 367.1 bits (941), Expect = 4.0e-98
Identity = 183/206 (88.83%), Postives = 194/206 (94.17%), Query Frame = 0


Query: 1   MTESGSAMTMSLAFAKEGFEIKSGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIKSLQ 60
           MTE+GS MTMSLAF KEGF IKSGGCGTIMRN+EMKI+N QTG SLPRNQ GEICI+S Q
Sbjct: 340 MTEAGSVMTMSLAFVKEGFGIKSGGCGTIMRNSEMKILNLQTGESLPRNQTGEICIRSSQ 399

Query: 61  IMKGYLNDEEATKGIIDKDGWLHTGDVGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEA 120
           +MKGYLNDE+ATK IID+DGWLHTGD+GFVDDDDE+FIVDRLKELIKYKGFQVAPAELEA
Sbjct: 400 MMKGYLNDEDATKAIIDEDGWLHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEA 459

Query: 121 LLISHGHIADAAVIPMKDEVAGEVPVAFIARSDGTNITEDEIKQYISKQVVFYKRINRVF 180
           LLISH HIADAAVIPM DEVAGEVPVAFI R DG+NITEDEIKQ+ISKQVVFYKRINRVF
Sbjct: 460 LLISHAHIADAAVIPMNDEVAGEVPVAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVF 519

Query: 181 FVDSIPKSPSGKILRRQLRALLAASI 207
           FVDSIPKSPSGKILRRQLRALLAASI
Sbjct: 520 FVDSIPKSPSGKILRRQLRALLAASI 545

[Close]

BLAST of Bhi02G000578 vs. NCBI nr
Match: XP_022135247.1 (4-coumarate--CoA ligase 1-like, partial [Momordica charantia])

HSP 1 Score: 366.7 bits (940), Expect = 5.2e-98
Identity = 184/208 (88.46%), Postives = 194/208 (93.27%), Query Frame = 0


Query: 1   MTESGSAMTMSLAFAKEGFEIKSGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIKSLQ 60
           MTE G   TMSLAFAKE FE+KSG CGT+MRN+EMKIINPQTGASLPRNQ GEICI+S Q
Sbjct: 99  MTE-GLVFTMSLAFAKERFEVKSGACGTLMRNSEMKIINPQTGASLPRNQPGEICIRSTQ 158

Query: 61  IMKGYLNDEEATKGIIDKDGWLHTGDVGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEA 120
            MKGYL+DEEATKGIIDK+GWLH+GD+GFVDDDDEVFIVDRLKELIKYKGFQVAPAELEA
Sbjct: 159 FMKGYLHDEEATKGIIDKNGWLHSGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEA 218

Query: 121 LLISHGHIADAAVIPMKDEVAGEVPVAFIARSDGTNITEDEIKQYISKQVVFYKRINRVF 180
           LLISHGHIADAAV+PMKDEVAGEVP AFI RSD +NI EDEIKQYISKQVVFYKRINRVF
Sbjct: 219 LLISHGHIADAAVVPMKDEVAGEVPAAFIVRSDSSNIAEDEIKQYISKQVVFYKRINRVF 278

Query: 181 FVDSIPKSPSGKILRRQLRALLAASIPN 209
           FVDSIPKS SGKILRRQLRALLAASIPN
Sbjct: 279 FVDSIPKSSSGKILRRQLRALLAASIPN 305

[Close]

BLAST of Bhi02G000578 vs. NCBI nr
Match: XP_022933955.1 (4-coumarate--CoA ligase 2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 358.6 bits (919), Expect = 1.4e-95
Identity = 179/208 (86.06%), Postives = 195/208 (93.75%), Query Frame = 0


Query: 1   MTESGSAMTMSLAFAKEGFEIKSGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIKSLQ 60
           MTE+GSA+T+SLAFAKE F IKSG CGT+MRN++MKI+NPQTGASLPRNQAGEI I+S  
Sbjct: 338 MTEAGSALTLSLAFAKERFPIKSGACGTLMRNSDMKIVNPQTGASLPRNQAGEIWIRSPH 397

Query: 61  IMKGYLNDEEATKGIIDKDGWLHTGDVGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEA 120
           +MKGYLN+EEATK IID+ GWLHTGD+GFVD+DDEVFIVDRLKELIKYKGFQVAPAELEA
Sbjct: 398 VMKGYLNNEEATKAIIDEHGWLHTGDIGFVDEDDEVFIVDRLKELIKYKGFQVAPAELEA 457

Query: 121 LLISHGHIADAAVIPMKDEVAGEVPVAFIARSDGTNITEDEIKQYISKQVVFYKRINRVF 180
           LLISH HIADAAVIPMK+EVAGEVPVAFI RS G+NITEDEIKQYISKQVVFYKRI+RVF
Sbjct: 458 LLISHTHIADAAVIPMKNEVAGEVPVAFIVRSGGSNITEDEIKQYISKQVVFYKRIHRVF 517

Query: 181 FVDSIPKSPSGKILRRQLRALLAASIPN 209
           FVDSIPKSPSGKILRRQLR LLAA IPN
Sbjct: 518 FVDSIPKSPSGKILRRQLRELLAARIPN 545

[Close]

BLAST of Bhi02G000578 vs. NCBI nr
Match: XP_023531498.1 (4-coumarate--CoA ligase 1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 356.7 bits (914), Expect = 5.4e-95
Identity = 178/208 (85.58%), Postives = 194/208 (93.27%), Query Frame = 0


Query: 1   MTESGSAMTMSLAFAKEGFEIKSGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIKSLQ 60
           MTE+GSA+T+SLAFAKE F IKSG CGT+MRN++MKI+NPQTGASLPRNQ GEI I+S  
Sbjct: 338 MTEAGSALTLSLAFAKERFPIKSGACGTLMRNSDMKIVNPQTGASLPRNQPGEIWIRSPH 397

Query: 61  IMKGYLNDEEATKGIIDKDGWLHTGDVGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEA 120
           +MKGYLN+EEATK IID+ GWLHTGD+GFVD+DDEVFIVDRLKELIKYKGFQVAPAELEA
Sbjct: 398 VMKGYLNNEEATKAIIDEHGWLHTGDIGFVDEDDEVFIVDRLKELIKYKGFQVAPAELEA 457

Query: 121 LLISHGHIADAAVIPMKDEVAGEVPVAFIARSDGTNITEDEIKQYISKQVVFYKRINRVF 180
           LLISH HIADAAVIPMK+EVAGEVPVAFI RS G+NITEDEIKQYISKQVVFYKRI+RVF
Sbjct: 458 LLISHTHIADAAVIPMKNEVAGEVPVAFIVRSGGSNITEDEIKQYISKQVVFYKRIHRVF 517

Query: 181 FVDSIPKSPSGKILRRQLRALLAASIPN 209
           FVDSIPKSPSGKILRRQLR LLAA IPN
Sbjct: 518 FVDSIPKSPSGKILRRQLRELLAARIPN 545

The following BLAST results are available for this feature:

BLAST of Bhi02G000578 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|O24146\|4CL2_TOBAC	8.8e-89	75.00	4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1	[more]
sp\|P31686\|4CL1_SOYBN	2.6e-88	75.48	4-coumarate--CoA ligase 1 (Fragment) OS=Glycine max OX=3847 PE=2 SV=1	[more]
sp\|O24145\|4CL1_TOBAC	7.5e-88	73.08	4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1	[more]
sp\|O24540\|4CL_VANPL	1.7e-87	75.12	4-coumarate--CoA ligase OS=Vanilla planifolia OX=51239 GN=4CL PE=3 SV=1	[more]
sp\|P31684\|4CL1_SOLTU	1.1e-86	74.04	4-coumarate--CoA ligase 1 OS=Solanum tuberosum OX=4113 GN=4CL1 PE=3 SV=1	[more]

BLAST of Bhi02G000578 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G21240.1	8.1e-85	72.60	4-coumarate:CoA ligase 2	[more]
AT1G51680.1	3.2e-81	69.95	4-coumarate:CoA ligase 1	[more]
AT3G21230.1	3.0e-79	69.80	4-coumarate:CoA ligase 5	[more]
AT1G65060.1	2.8e-77	66.34	4-coumarate:CoA ligase 3	[more]
AT4G05160.1	2.5e-54	55.68	AMP-dependent synthetase and ligase family protein	[more]

BLAST of Bhi02G000578 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3BWP0\|A0A1S3BWP0_CUCME	4.8e-100	89.81	4-coumarate--CoA ligase 1-like OS=Cucumis melo OX=3656 GN=LOC103494274 PE=4 SV=1	[more]
tr\|A0A0A0LWT6\|A0A0A0LWT6_CUCSA	2.6e-98	88.83	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G423020 PE=4 SV=1	[more]
tr\|A0A068FPP7\|A0A068FPP7_GARMA	7.7e-90	77.40	4-coumarate coenzymeA ligase (Fragment) OS=Garcinia mangostana OX=58228 GN=4CL P...	[more]
tr\|I6PD12\|I6PD12_9MAGN	1.7e-89	76.92	4-coumarate-CoA ligase OS=Cinnamomum osmophloeum OX=258907 GN=4Cl1 PE=2 SV=1	[more]
tr\|A0A1R3G3Z5\|A0A1R3G3Z5_COCAP	2.9e-89	76.92	AMP-dependent synthetase/ligase OS=Corchorus capsularis OX=210143 GN=CCACVL1_290...	[more]

BLAST of Bhi02G000578 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008453615.1	7.3e-100	89.81	PREDICTED: 4-coumarate--CoA ligase 1-like [Cucumis melo]	[more]
XP_004146311.1	4.0e-98	88.83	PREDICTED: 4-coumarate--CoA ligase 1-like [Cucumis sativus] >KGN65444.1 hypothet...	[more]
XP_022135247.1	5.2e-98	88.46	4-coumarate--CoA ligase 1-like, partial [Momordica charantia]	[more]
XP_022933955.1	1.4e-95	86.06	4-coumarate--CoA ligase 2-like isoform X1 [Cucurbita moschata]	[more]
XP_023531498.1	5.4e-95	85.58	4-coumarate--CoA ligase 1-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity

Vocabulary: **INTERPRO**
Term	Definition
IPR000873	AMP-dep_Synth/Lig
IPR025110	AMP-bd_C

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
biological_process	GO:0008150	biological_process
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016874	ligase activity
molecular_function	GO:0003674	molecular_function
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0016207	4-coumarate-CoA ligase activity
molecular_function	GO:0016491	oxidoreductase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi02M000578	Bhi02M000578	mRNA

InterPro

Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR025110	AMP-binding enzyme, C-terminal domain	PFAM	PF13193	AMP-binding_C	coord: 117..192 e-value: 2.6E-16 score: 60.2
IPR000873	AMP-dependent synthetase/ligase	PFAM	PF00501	AMP-binding	coord: 1..108 e-value: 1.3E-30 score: 106.3
None	No IPR available	GENE3D	G3DSA:3.30.300.30		coord: 104..205 e-value: 5.1E-30 score: 106.1
None	No IPR available	GENE3D	G3DSA:3.40.50.12780		coord: 1..103 e-value: 3.1E-39 score: 136.9
None	No IPR available	PANTHER	PTHR24096	FAMILY NOT NAMED	coord: 1..205
None	No IPR available	PANTHER	PTHR24096:SF207	4-COUMARATE--COA LIGASE 1-RELATED	coord: 1..205
None	No IPR available	SUPERFAMILY	SSF56801	Acetyl-CoA synthetase-like	coord: 1..204

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Bhi02G000578	Carg15247	Silver-seed gourd	carwgoB0925

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Bhi02G000578	Bhi06G001173	Wax gourd	wgowgoB052

The following block(s) are covering this gene:


Gene	Organism	Block
Bhi02G000578	Cucurbita pepo (Zucchini)	cpewgoB0466
Bhi02G000578	Cucumber (Gy14) v1	cgywgoB120
Bhi02G000578	Cucumber (Gy14) v1	cgywgoB123
Bhi02G000578	Cucumber (Gy14) v2	cgybwgoB095
Bhi02G000578	Cucumber (Gy14) v2	cgybwgoB204
Bhi02G000578	Cucurbita maxima (Rimu)	cmawgoB0076
Bhi02G000578	Cucurbita maxima (Rimu)	cmawgoB0150
Bhi02G000578	Cucurbita maxima (Rimu)	cmawgoB0973
Bhi02G000578	Cucurbita moschata (Rifu)	cmowgoB0070
Bhi02G000578	Cucurbita moschata (Rifu)	cmowgoB0145
Bhi02G000578	Cucurbita moschata (Rifu)	cmowgoB0969
Bhi02G000578	Cucurbita pepo (Zucchini)	cpewgoB0896
Bhi02G000578	Wild cucumber (PI 183967)	cpiwgoB101
Bhi02G000578	Wild cucumber (PI 183967)	cpiwgoB212
Bhi02G000578	Cucumber (Chinese Long) v3	cucwgoB106
Bhi02G000578	Cucumber (Chinese Long) v3	cucwgoB218
Bhi02G000578	Cucumber (Chinese Long) v2	cuwgoB097
Bhi02G000578	Cucumber (Chinese Long) v2	cuwgoB212
Bhi02G000578	Bottle gourd (USVL1VR-Ls)	lsiwgoB141
Bhi02G000578	Bottle gourd (USVL1VR-Ls)	lsiwgoB523
Bhi02G000578	Melon (DHL92) v3.6.1	medwgoB306
Bhi02G000578	Melon (DHL92) v3.5.1	mewgoB307
Bhi02G000578	Watermelon (Charleston Gray)	wcgwgoB547
Bhi02G000578	Watermelon (97103) v2	wgowmbB655
Bhi02G000578	Watermelon (97103) v1	wgowmB684
Bhi02G000578	Silver-seed gourd	carwgoB0074
Bhi02G000578	Silver-seed gourd	carwgoB0711