Bhi02G000335 (gene) Wax gourd

NameBhi02G000335
Typegene
OrganismBenincasa hispida (Wax gourd)
DescriptionCASP-like protein
Locationchr2 : 8231088 .. 8234468 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CACAAATTCATTACAAAAACGACGTCGTAAGAAGGGAGTAAGAGCGCCACATTCAACCAGACGAAAGATTAGAAATTAAAATAAATAAATAAACGGAATCGTAGGCTTCGCTTGGCAGCTCACTTTACTTTGTCGGAGAGGCTCTAAAATACGGTCACATACACTGTTCTCAAAACTTTGTCATCAAATTACAATCTTCCACGTCACACTAAACATTACGTGTATCAGAGTCCTTGTGATTATTCCCATTCTGAACGTCAGATTATTTCACCTTGTTCAAAATTGTGTACACTGTACCACACTGGAATATATTCTCCTCCGACTTTAATCCCATTAATCAAAGGATGCCACGTGGCTTTAAATTTACAAACGCGAACTTAGAGACGAGTCCCACTAAAGTCCTCCGCTTTTCCTTTCTTTTTTTCCGACTTCCCAAACAGCCTTTCCTTTTTCCTTTTTCCTTTTTCTTTTTTTCCTTTTTCTTTTTCCAAAATAATTTCATTAAAATCCCTCAAATTTCTAACAAAAAATGATTGAAACTAAATTGAAAGCTCAAAGATCGAACGACTCGTCGTCCATGAAGAAATCGTCGTCAAGAAATTCCGAGTCATCGGTTCACAGTCATATGGAGTCTCCTCATTCACCATTGCGTTTCCATTCCCCGCTCCGATCCGACGCCGGTGACCCTCCGGAGACCCCTCCTTATGCCTCTCCCGATACCTCCCCTGAGAAGCTGCCGGATAACTCCAAGGCGATTGTTGTTGTTGATGTTGTTGATAAGAGTACTCAGTTCTCTCCCCTTCCGTCTCCTTATGCCGGCTCTCGGATGCCGCCGGAGAACGTGAACTTTACTGGAGATAGGTCGACTTCTTCTAAGGTTATGTTTAATCGAGCAATGAAAGAAGAAGTGCCGCAGTCGGTTACCAAAGTAGGGCCGGCTGGTGGTGGAGGTGAGGAGAATGGTGGTGGAGGTGGAGGTGGAGGTGGAAGATCGCCGGTGAAGCAAATGGCGCCGATGTCGAGGAGGTCGAAAGACGATGCGTTGACGAAGGCGGCTTTAGGTTTTAGAGTTTGTGAAGTGGCGGTTTGCTTGATTTCGTTTTCAGTTATGGCGTCTGATAAAACTCAGGGTTGGAGCGGCGACTCCTTTGATCGTTACAAGGAATACAGGTTTTTTTCTTCTTCTTTTGATTCTTGACTCCTGTTTTACTACCTAAGTGCTCTTAAACTAGAGAATTGGAGTTGCTTTTGGCTGGTGTGAAATTTACATTTTCATTTTGTTATCTTCTTCCTGAATTGATGAAACGTGTCTGTTTCTTCAATTCCTTCGGTTTAATCTATCTAGACTTAACTATTTTAGTTCACTGTGCACTTGGCGTCAACTACATATGCATACCTAATCTTGAACTTGTAGTACGGCCAGTGGGACCTCATAGGTTTTTACCGTACCAATTAGCTATCCATTCATAGGTTTTTACTATAATTTCATTCCAAGGAGAATCAGAGCCTGAAACAATTATTAGGATTAATGGTTTTGAGATCCTTCTGTTACTTGTATACGACATTGTCATCACATGATTTGGTGCTTTAATTGTAGTCTTCAATTTCAAGGTACGTCTTATTTTCAATGACGAATGCCATGTTAAAAATGGAGCTGTCACCTTCGTTCCACCCTATTGATTGTCAAGTTTCAATGATATACAGTCCAAATGAATCCTTTCACTACACTATTTAATTCAGTCTGACCCACAACTTTTTAAGACATGTTCCAAGTCTCTAACATAACAACTTTTATATGTCCTCCCCCGAGTCGTTTGCTGCTTTAAGTTACAAGTCTTTTGTTTTCAATTCTCATGTTGGTTTTTGATATAATTTCAAGTTTGTGTACTGTGAGTGAAGGATTGTGGTGGAAGCAATCTCACTTATCAGGGTTGTTCCTTGACTTCCATGCCTTTTGGTGATCAATCATGTGGCATTATTCTTAGCTTCACTTGTATTGCAAGAATACATCAATTTTTCATGCTATAACATGAAAGCCGTACTTAAGGGATATATTTCATATTCATTTTCTTCAAATTATTTGAAATGGTCTCTTTTTTGCAATTTTCTAGAAGTCTATATGTGTTTTCCTGTCTTATATTATCTAATCAAGTTTAATTGTCAACAGGTACTGCCTCACTGTGAATATAATTGCTTTCGTGTATGCGGCATTTCAAGCATTTGATCTTGTCTTTACTCTGATCAAGAAGAACCATATGATACGACATCAATTTCGTTGTTATTTTGACTTCTTCATGGATCAGGTTAGTTCCGCAAATATGCAAAGCTGAATGAATATTGTTTGACTCATGCTTTCACTGCTAGTCTGCTACAATGAATGTGATTCTATGGTCAAACTGAAAGGATTGCTTAGTACAATTCTGTTACCTGATGAATAGCATACTTGGGCTAGAAGAAACTCGCTGAATTGCATAGAGAAACCTTTTTCTGCTTCATAACTTGACTAAGCTTTACTTGATTTAAAACGATCATTCAAGTGGGGACGAACTTGATTTTACATCTGCTGATCACTTGTCTCTTTTAATGATTGATTGTGTCCTTGCGTGAGTGTAGATTTGGTTTCTCAAATATCGAGGGGATTATTGGGATGGACCTTTTTAAGGGCTTAAAAAGTGTTGCTAAATGAAGAAAAAAAATGATTTGAAGCACTTAGAAATCCAATCCAAACAGACGATCTTACTTGAACAAGATTTATTCTATAGGTTGTACAACTATGTTCTTGAGTTCTAAAGAAAGTCAATCCAGAGATGAAATCATAGTTGTTAATCATCAAGTCTTCACCCTCTCGAGTCTCAACCAGAAATTTCTTGATATCCCAAGATTGTTAGCTACTTTGCTTGCTTTGGTTTCTGTATTAACTAATGATGCAATCCTTGTTTTATTGATTGTAATGGCAGGTATTGGCATATCTTCTCATATCATCGTCTTCCTCGGCCGCAACGCGTGTTGATGACTGGCAATCGAATTGGGGAAAAGACGAGTTCACACAGTTGGCCAGTGCATCGGTCAGCATGTCCTTCCTTGCCTTTGTTGCTTTTGCTTTTAGTTCACTCATCTCTGGTTACAACCTTTGTACCCGCGATCCTGCATGATATTGAAATCTCAGGCCTGTTTGTTTTTACTTGAAAAGAGAAAATTTCATATTGATTGTCAACACAAAATTATTCTTTTCTTTATGTTGTACAGAGATATCATAGCTTAGCTTTGACCTTACAATAAAGAGGATACCAAATTGTAAAATTGTTGGATTGTTTTTATTGCATTTCTTTTATATATATATAAATGAAGCTTCTTTTGCTCTAGCAATAAATTCATCATAAA

mRNA sequence

CACAAATTCATTACAAAAACGACGTCGTAAGAAGGGAGTAAGAGCGCCACATTCAACCAGACGAAAGATTAGAAATTAAAATAAATAAATAAACGGAATCGTAGGCTTCGCTTGGCAGCTCACTTTACTTTGTCGGAGAGGCTCTAAAATACGGTCACATACACTGTTCTCAAAACTTTGTCATCAAATTACAATCTTCCACGTCACACTAAACATTACGTGTATCAGAGTCCTTGTGATTATTCCCATTCTGAACGTCAGATTATTTCACCTTGTTCAAAATTGTGTACACTGTACCACACTGGAATATATTCTCCTCCGACTTTAATCCCATTAATCAAAGGATGCCACGTGGCTTTAAATTTACAAACGCGAACTTAGAGACGAGTCCCACTAAAGTCCTCCGCTTTTCCTTTCTTTTTTTCCGACTTCCCAAACAGCCTTTCCTTTTTCCTTTTTCCTTTTTCTTTTTTTCCTTTTTCTTTTTCCAAAATAATTTCATTAAAATCCCTCAAATTTCTAACAAAAAATGATTGAAACTAAATTGAAAGCTCAAAGATCGAACGACTCGTCGTCCATGAAGAAATCGTCGTCAAGAAATTCCGAGTCATCGGTTCACAGTCATATGGAGTCTCCTCATTCACCATTGCGTTTCCATTCCCCGCTCCGATCCGACGCCGGTGACCCTCCGGAGACCCCTCCTTATGCCTCTCCCGATACCTCCCCTGAGAAGCTGCCGGATAACTCCAAGGCGATTGTTGTTGTTGATGTTGTTGATAAGAGTACTCAGTTCTCTCCCCTTCCGTCTCCTTATGCCGGCTCTCGGATGCCGCCGGAGAACGTGAACTTTACTGGAGATAGGTCGACTTCTTCTAAGGTTATGTTTAATCGAGCAATGAAAGAAGAAGTGCCGCAGTCGGTTACCAAAGTAGGGCCGGCTGGTGGTGGAGGTGAGGAGAATGGTGGTGGAGGTGGAGGTGGAGGTGGAAGATCGCCGGTGAAGCAAATGGCGCCGATGTCGAGGAGGTCGAAAGACGATGCGTTGACGAAGGCGGCTTTAGGTTTTAGAGTTTGTGAAGTGGCGGTTTGCTTGATTTCGTTTTCAGTTATGGCGTCTGATAAAACTCAGGGTTGGAGCGGCGACTCCTTTGATCGTTACAAGGAATACAGGTACTGCCTCACTGTGAATATAATTGCTTTCGTGTATGCGGCATTTCAAGCATTTGATCTTGTCTTTACTCTGATCAAGAAGAACCATATGATACGACATCAATTTCGTTGTTATTTTGACTTCTTCATGGATCAGGTATTGGCATATCTTCTCATATCATCGTCTTCCTCGGCCGCAACGCGTGTTGATGACTGGCAATCGAATTGGGGAAAAGACGAGTTCACACAGTTGGCCAGTGCATCGGTCAGCATGTCCTTCCTTGCCTTTGTTGCTTTTGCTTTTAGTTCACTCATCTCTGGTTACAACCTTTGTACCCGCGATCCTGCATGATATTGAAATCTCAGGCCTGTTTGTTTTTACTTGAAAAGAGAAAATTTCATATTGATTGTCAACACAAAATTATTCTTTTCTTTATGTTGTACAGAGATATCATAGCTTAGCTTTGACCTTACAATAAAGAGGATACCAAATTGTAAAATTGTTGGATTGTTTTTATTGCATTTCTTTTATATATATATAAATGAAGCTTCTTTTGCTCTAGCAATAAATTCATCATAAA

Coding sequence (CDS)

ATGATTGAAACTAAATTGAAAGCTCAAAGATCGAACGACTCGTCGTCCATGAAGAAATCGTCGTCAAGAAATTCCGAGTCATCGGTTCACAGTCATATGGAGTCTCCTCATTCACCATTGCGTTTCCATTCCCCGCTCCGATCCGACGCCGGTGACCCTCCGGAGACCCCTCCTTATGCCTCTCCCGATACCTCCCCTGAGAAGCTGCCGGATAACTCCAAGGCGATTGTTGTTGTTGATGTTGTTGATAAGAGTACTCAGTTCTCTCCCCTTCCGTCTCCTTATGCCGGCTCTCGGATGCCGCCGGAGAACGTGAACTTTACTGGAGATAGGTCGACTTCTTCTAAGGTTATGTTTAATCGAGCAATGAAAGAAGAAGTGCCGCAGTCGGTTACCAAAGTAGGGCCGGCTGGTGGTGGAGGTGAGGAGAATGGTGGTGGAGGTGGAGGTGGAGGTGGAAGATCGCCGGTGAAGCAAATGGCGCCGATGTCGAGGAGGTCGAAAGACGATGCGTTGACGAAGGCGGCTTTAGGTTTTAGAGTTTGTGAAGTGGCGGTTTGCTTGATTTCGTTTTCAGTTATGGCGTCTGATAAAACTCAGGGTTGGAGCGGCGACTCCTTTGATCGTTACAAGGAATACAGGTACTGCCTCACTGTGAATATAATTGCTTTCGTGTATGCGGCATTTCAAGCATTTGATCTTGTCTTTACTCTGATCAAGAAGAACCATATGATACGACATCAATTTCGTTGTTATTTTGACTTCTTCATGGATCAGGTATTGGCATATCTTCTCATATCATCGTCTTCCTCGGCCGCAACGCGTGTTGATGACTGGCAATCGAATTGGGGAAAAGACGAGTTCACACAGTTGGCCAGTGCATCGGTCAGCATGTCCTTCCTTGCCTTTGTTGCTTTTGCTTTTAGTTCACTCATCTCTGGTTACAACCTTTGTACCCGCGATCCTGCATGA

Protein sequence

MIETKLKAQRSNDSSSMKKSSSRNSESSVHSHMESPHSPLRFHSPLRSDAGDPPETPPYASPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYAGSRMPPENVNFTGDRSTSSKVMFNRAMKEEVPQSVTKVGPAGGGGEENGGGGGGGGGRSPVKQMAPMSRRSKDDALTKAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFDLVFTLIKKNHMIRHQFRCYFDFFMDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQLASASVSMSFLAFVAFAFSSLISGYNLCTRDPA
BLAST of Bhi02G000335 vs. Swiss-Prot
Match: sp|D7MMW4|CSPLJ_ARALL (CASP-like protein 4A2 OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALYDRAFT_919556 PE=3 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 2.6e-63
Identity = 147/303 (48.51%), Postives = 191/303 (63.04%), Query Frame = 0

Query: 30  HSHMESPHSPLRFHSPLRSDAGDPPETXXXXXXXXXXXXXXXNSKAIVVVDVVDKSTQFS 89
           +S+ ESPHSPLRFHSPL SDAGD PE+               N K+IV     +K TQFS
Sbjct: 14  YSYNESPHSPLRFHSPL-SDAGDLPES--RYVSPEGSPFKIENPKSIV---AGNKLTQFS 73

Query: 90  PLPS-------------PYAGSRMPPENVNFTGDRSTSSKVMFNRAMKEEVPQSVTKVGP 149
           PLPS                 +R+P   +N + D+S SS V+ N  ++E+  Q+ T+   
Sbjct: 74  PLPSXXXXXXXXXXXXXXXRNARVP---MNSSLDKSPSSMVVQNSWVREDGQQNTTRKAG 133

Query: 150 AXXXXXXXXXXXXXXXXXXPVKQMAPMSRRSKDDALTKAALGFRVCEVAVCLISFSVMAS 209
           A                         ++R  +DD ++  ALGFR+ EV +C+ISFS+MA+
Sbjct: 134 APMNGEE--------------SATTAVNRARRDDLVSVTALGFRITEVILCVISFSIMAA 193

Query: 210 DKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFDLVFTLIKKNHMIRHQFRCYFDFF 269
           DKTQGWSGDS+DRYKEYRYCL VN+IAFVY+AF+A D    + K+++M+   F   F F 
Sbjct: 194 DKTQGWSGDSYDRYKEYRYCLAVNVIAFVYSAFEACDAACYMAKESYMMNCGFHDLFVFS 253

Query: 270 MDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQLASASVSMSFLAFVAFAFSSLISGYN 320
           MDQ+LAYLL+S+SS AATRVDDW SNWGKDEFTQ+A+AS+++SFLAF AFA S+LIS Y 
Sbjct: 254 MDQLLAYLLMSASSCAATRVDDWVSNWGKDEFTQMATASIAVSFLAFGAFAVSALISSYR 293

BLAST of Bhi02G000335 vs. Swiss-Prot
Match: sp|Q501G6|CSPLY_ARATH (CASP-like protein 4A2 OS=Arabidopsis thaliana OX=3702 GN=At5g62820 PE=2 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 7.1e-61
Identity = 144/299 (48.16%), Postives = 180/299 (60.20%), Query Frame = 0

Query: 31  SHMESPHSPLRFHSPLRSDAGDPPETXXXXXXXXXXXXXXXNSKAIVVVDVVDKSTQFSP 90
           S+ ESPHSPLRFHSPL SDAGD PE+               N K+IV     +K TQFSP
Sbjct: 15  SYNESPHSPLRFHSPL-SDAGDLPES--RYVSPEGSPFKIENPKSIV---AGNKLTQFSP 74

Query: 91  LPSP----------YAGSRMPPENVNFTGDRSTSSKVMFNRAMKEEVPQSVTKVGPAXXX 150
           LPSP                             SS V+ N  ++E+  Q+ T+   A   
Sbjct: 75  LPSPIXXXXXXXXXXXXXXXXXXXXXXXXXXXPSSMVVHNSWVREDGGQTTTRKAGAPIN 134

Query: 151 XXXXXXXXXXXXXXXPVKQMAPMSRRSKDDALTKAALGFRVCEVAVCLISFSVMASDKTQ 210
                                 ++R   DD ++  ALGFR+ EV +C+ISFS+MA+DKTQ
Sbjct: 135 GEE--------------STRTTVNRARGDDLVSLTALGFRITEVILCVISFSIMAADKTQ 194

Query: 211 GWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFDLVFTLIKKNHMIRHQFRCYFDFFMDQV 270
           GWSGDS+DRYKEYRYCL VN+IAFVY+AF+A D    + K+++MI   F   F F MDQ+
Sbjct: 195 GWSGDSYDRYKEYRYCLAVNVIAFVYSAFEACDAACYIAKESYMINCGFHDLFVFSMDQL 254

Query: 271 LAYLLISSSSSAATRVDDWQSNWGKDEFTQLASASVSMSFLAFVAFAFSSLISGYNLCT 320
           LAYLL+S+SS AATRVDDW SNWGKDEFTQ+A+AS+++SFLAF AFA S+LIS Y L T
Sbjct: 255 LAYLLMSASSCAATRVDDWVSNWGKDEFTQMATASIAVSFLAFGAFAVSALISSYRLFT 293

BLAST of Bhi02G000335 vs. Swiss-Prot
Match: sp|Q84WP5|CSPL8_ARATH (CASP-like protein 4A3 OS=Arabidopsis thaliana OX=3702 GN=At2g36330 PE=2 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 4.0e-56
Identity = 105/154 (68.18%), Postives = 135/154 (87.66%), Query Frame = 0

Query: 165 RRSKDDALTK-AALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIA 224
           RRS+ + + K +ALGFR+ EV + LISFS+MA+DKT+GWSGDSFDRYKEYR+CL+VN++A
Sbjct: 124 RRSRREEVVKFSALGFRLSEVVLALISFSIMAADKTKGWSGDSFDRYKEYRFCLSVNVVA 183

Query: 225 FVYAAFQAFDLVFTLIKKNHMIRHQFRCYFDFFMDQVLAYLLISSSSSAATRVDDWQSNW 284
           FVY++FQA DL + L+K+ H+I H  R  F+F +DQVLAYLL+S+S++A TRVDDW SNW
Sbjct: 184 FVYSSFQACDLAYHLVKEKHLISHHLRPLFEFIIDQVLAYLLMSASTAAVTRVDDWVSNW 243

Query: 285 GKDEFTQLASASVSMSFLAFVAFAFSSLISGYNL 318
           GKDEFT++ASAS++MSFLAF+AFAFSSLISGYNL
Sbjct: 244 GKDEFTEMASASIAMSFLAFLAFAFSSLISGYNL 277

BLAST of Bhi02G000335 vs. Swiss-Prot
Match: sp|D7LIR2|CSPLI_ARALL (CASP-like protein 4A3 OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALYDRAFT_482607 PE=3 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 8.9e-56
Identity = 103/158 (65.19%), Postives = 134/158 (84.81%), Query Frame = 0

Query: 165 RRSKDDALTK-AALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIA 224
           RRS+ + + K  ALGFR+ EV + LISFS+MA+DKT+GWSGDSFDRYKEYR+CL+VN++A
Sbjct: 117 RRSRREEIVKFVALGFRLSEVVLALISFSIMAADKTKGWSGDSFDRYKEYRFCLSVNVVA 176

Query: 225 FVYAAFQAFDLVFTLIKKNHMIRHQFRCYFDFFMDQVLAYLLISSSSSAATRVDDWQSNW 284
           F+YA+FQA DL + L+K+ H+I H  R  F+F +DQVLAYLL+ +S++A TRVDDW SNW
Sbjct: 177 FIYASFQACDLAYHLVKEKHLISHHLRPLFEFIIDQVLAYLLMCASTAAVTRVDDWVSNW 236

Query: 285 GKDEFTQLASASVSMSFLAFVAFAFSSLISGYNLCTRD 322
           GKD+FT++ASAS++MSFL F+AFAFSSLISGYNL  +D
Sbjct: 237 GKDDFTEMASASIAMSFLTFLAFAFSSLISGYNLFNQD 274

BLAST of Bhi02G000335 vs. Swiss-Prot
Match: sp|Q9FNE8|CSPLV_ARATH (CASP-like protein 4A1 OS=Arabidopsis thaliana OX=3702 GN=At5g40300 PE=2 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 3.6e-41
Identity = 83/147 (56.46%), Postives = 106/147 (72.11%), Query Frame = 0

Query: 172 LTKAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQA 231
           + KA LGFRV     CL+SFSVM SD+ +GW+ DSF  YKE+R+CL  N+I FVY+ F  
Sbjct: 119 IRKALLGFRVIAFVSCLVSFSVMVSDRDKGWAHDSFYNYKEFRFCLAANVIGFVYSGFMI 178

Query: 232 FDLVFTLIKKNHMIRHQFRCYFDFFMDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQL 291
            DLV+ L       RH  R + +F +DQ+LAYLL S+S+SA+ RVDDWQSNWG D+F  L
Sbjct: 179 CDLVYLLSTSIRRSRHNLRHFLEFGLDQMLAYLLASASTSASIRVDDWQSNWGADKFPDL 238

Query: 292 ASASVSMSFLAFVAFAFSSLISGYNLC 319
           A ASV++S+++FVAFAF SL SGY LC
Sbjct: 239 ARASVALSYVSFVAFAFCSLASGYALC 265

BLAST of Bhi02G000335 vs. TAIR10
Match: AT5G62820.1 (Uncharacterised protein family (UPF0497))

HSP 1 Score: 235.7 bits (600), Expect = 3.9e-62
Identity = 144/299 (48.16%), Postives = 180/299 (60.20%), Query Frame = 0

Query: 31  SHMESPHSPLRFHSPLRSDAGDPPETXXXXXXXXXXXXXXXNSKAIVVVDVVDKSTQFSP 90
           S+ ESPHSPLRFHSPL SDAGD PE+               N K+IV     +K TQFSP
Sbjct: 15  SYNESPHSPLRFHSPL-SDAGDLPES--RYVSPEGSPFKIENPKSIV---AGNKLTQFSP 74

Query: 91  LPSP----------YAGSRMPPENVNFTGDRSTSSKVMFNRAMKEEVPQSVTKVGPAXXX 150
           LPSP                             SS V+ N  ++E+  Q+ T+   A   
Sbjct: 75  LPSPIXXXXXXXXXXXXXXXXXXXXXXXXXXXPSSMVVHNSWVREDGGQTTTRKAGAPIN 134

Query: 151 XXXXXXXXXXXXXXXPVKQMAPMSRRSKDDALTKAALGFRVCEVAVCLISFSVMASDKTQ 210
                                 ++R   DD ++  ALGFR+ EV +C+ISFS+MA+DKTQ
Sbjct: 135 GEE--------------STRTTVNRARGDDLVSLTALGFRITEVILCVISFSIMAADKTQ 194

Query: 211 GWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFDLVFTLIKKNHMIRHQFRCYFDFFMDQV 270
           GWSGDS+DRYKEYRYCL VN+IAFVY+AF+A D    + K+++MI   F   F F MDQ+
Sbjct: 195 GWSGDSYDRYKEYRYCLAVNVIAFVYSAFEACDAACYIAKESYMINCGFHDLFVFSMDQL 254

Query: 271 LAYLLISSSSSAATRVDDWQSNWGKDEFTQLASASVSMSFLAFVAFAFSSLISGYNLCT 320
           LAYLL+S+SS AATRVDDW SNWGKDEFTQ+A+AS+++SFLAF AFA S+LIS Y L T
Sbjct: 255 LAYLLMSASSCAATRVDDWVSNWGKDEFTQMATASIAVSFLAFGAFAVSALISSYRLFT 293

BLAST of Bhi02G000335 vs. TAIR10
Match: AT2G36330.1 (Uncharacterised protein family (UPF0497))

HSP 1 Score: 219.9 bits (559), Expect = 2.2e-57
Identity = 105/154 (68.18%), Postives = 135/154 (87.66%), Query Frame = 0

Query: 165 RRSKDDALTK-AALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIA 224
           RRS+ + + K +ALGFR+ EV + LISFS+MA+DKT+GWSGDSFDRYKEYR+CL+VN++A
Sbjct: 124 RRSRREEVVKFSALGFRLSEVVLALISFSIMAADKTKGWSGDSFDRYKEYRFCLSVNVVA 183

Query: 225 FVYAAFQAFDLVFTLIKKNHMIRHQFRCYFDFFMDQVLAYLLISSSSSAATRVDDWQSNW 284
           FVY++FQA DL + L+K+ H+I H  R  F+F +DQVLAYLL+S+S++A TRVDDW SNW
Sbjct: 184 FVYSSFQACDLAYHLVKEKHLISHHLRPLFEFIIDQVLAYLLMSASTAAVTRVDDWVSNW 243

Query: 285 GKDEFTQLASASVSMSFLAFVAFAFSSLISGYNL 318
           GKDEFT++ASAS++MSFLAF+AFAFSSLISGYNL
Sbjct: 244 GKDEFTEMASASIAMSFLAFLAFAFSSLISGYNL 277

BLAST of Bhi02G000335 vs. TAIR10
Match: AT5G40300.1 (Uncharacterised protein family (UPF0497))

HSP 1 Score: 170.2 bits (430), Expect = 2.0e-42
Identity = 83/147 (56.46%), Postives = 106/147 (72.11%), Query Frame = 0

Query: 172 LTKAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQA 231
           + KA LGFRV     CL+SFSVM SD+ +GW+ DSF  YKE+R+CL  N+I FVY+ F  
Sbjct: 119 IRKALLGFRVIAFVSCLVSFSVMVSDRDKGWAHDSFYNYKEFRFCLAANVIGFVYSGFMI 178

Query: 232 FDLVFTLIKKNHMIRHQFRCYFDFFMDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQL 291
            DLV+ L       RH  R + +F +DQ+LAYLL S+S+SA+ RVDDWQSNWG D+F  L
Sbjct: 179 CDLVYLLSTSIRRSRHNLRHFLEFGLDQMLAYLLASASTSASIRVDDWQSNWGADKFPDL 238

Query: 292 ASASVSMSFLAFVAFAFSSLISGYNLC 319
           A ASV++S+++FVAFAF SL SGY LC
Sbjct: 239 ARASVALSYVSFVAFAFCSLASGYALC 265

BLAST of Bhi02G000335 vs. TAIR10
Match: AT2G38480.1 (Uncharacterised protein family (UPF0497))

HSP 1 Score: 83.2 bits (204), Expect = 3.3e-16
Identity = 58/156 (37.18%), Postives = 92/156 (58.97%), Query Frame = 0

Query: 165 RRSKDDALTKAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAF 224
           R  ++D + KA+   R   +   LI+F +M S+K  G+ G +F+ Y+EYRY L ++II+ 
Sbjct: 37  RWKREDLIKKASPITRGICLLFSLIAFLIMVSNK-HGY-GRNFNDYEEYRYVLAISIIST 96

Query: 225 VYAAFQAFDLVFTLIKKNHMIRHQFRCYFDFFMDQVLAYLLISSSSSAATRVDDWQSNWG 284
           +Y A+Q     F    K  +   +     DF  DQ++AYLLIS++SSA    + ++   G
Sbjct: 97  LYTAWQ----TFAHFSKREIFDRRTSILVDFSGDQIVAYLLISAASSAIPLTNIFRE--G 156

Query: 285 KDE-FTQLASASVSMSFLAFVAFAFSSLISGYNLCT 320
           +D  FT  A++++SM+  AF+A A S+L SGY L T
Sbjct: 157 QDNIFTDSAASAISMAIFAFIALALSALFSGYKLST 184

BLAST of Bhi02G000335 vs. TAIR10
Match: AT4G11655.1 (Uncharacterised protein family (UPF0497))

HSP 1 Score: 75.5 bits (184), Expect = 6.8e-14
Identity = 51/144 (35.42%), Postives = 69/144 (47.92%), Query Frame = 0

Query: 181 VCEVAVCLISFSVMASDKTQ-GWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFDLVFTLI 240
           +C V+   ++ +V    K     +  SF  Y E  YC  V +I FVY + Q F  V  + 
Sbjct: 66  LCFVSALSLAVNVQRPSKRHLTQNSSSFASYPELLYCFGVAVIGFVYTSLQTFKGVCDIT 125

Query: 241 KKNHMIRHQFRCYFDFFMDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQLAS---ASV 300
            +  +I      Y  F  DQV+ YLL+SSSS A      W  +  +D    L +    SV
Sbjct: 126 HRGVLISEPLSDYISFIFDQVICYLLVSSSSVAIA----WIQHINEDAIKTLRNNSIVSV 185

Query: 301 SMSFLAFVAFAFSSLISGYNLCTR 321
           SMSF AF+    S L+SGY LC R
Sbjct: 186 SMSFSAFLVLTLSGLLSGYKLCKR 205

BLAST of Bhi02G000335 vs. TrEMBL
Match: tr|A0A1S3BWI9|A0A1S3BWI9_CUCME (CASP-like protein OS=Cucumis melo OX=3656 GN=LOC103493912 PE=3 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 2.5e-140
Identity = 298/330 (90.30%), Postives = 299/330 (90.61%), Query Frame = 0

Query: 1   MIETKLKAQRSND-SSSMKKXXXXXXXXXVHSHMESPHSPLRFHSPLRSDAGDPPETXXX 60
           MIETKL+AQRSND SSS   XXXXXXXX VHSHMESPHSPLRFHSPLRSDAGDP ET   
Sbjct: 1   MIETKLRAQRSNDSSSSXXXXXXXXXXXSVHSHMESPHSPLRFHSPLRSDAGDPMETPPY 60

Query: 61  XXXXXXXXXXXXNSKAIVVVDVVDKSTQFSPLPSPYAGSRMPPENVNFTGDRSTSSKVMF 120
                       NSKAIVVVDVVDKSTQFSPLPSPYAGSR P ENVNF GDRSTSSKVMF
Sbjct: 61  ASPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYAGSRKPLENVNFVGDRSTSSKVMF 120

Query: 121 NRAMKEEVPQSVTKVGPA------XXXXXXXXXXXXXXXXXXPVKQMAPMSRRSKDDALT 180
           NRAMKEEVPQSVTKVGPA      XXXXXXXXXXXXXXXXXXPVKQMAPMSRRSKDDAL 
Sbjct: 121 NRAMKEEVPQSVTKVGPAGVGGVXXXXXXXXXXXXXXXXXXXPVKQMAPMSRRSKDDALM 180

Query: 181 KAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFD 240
           KAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFD
Sbjct: 181 KAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFD 240

Query: 241 LVFTLIKKNHMIRHQFRCYFDFFMDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQLAS 300
           LVFTLIKKNHMIRHQFRCYFDFFMDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQLAS
Sbjct: 241 LVFTLIKKNHMIRHQFRCYFDFFMDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQLAS 300

Query: 301 ASVSMSFLAFVAFAFSSLISGYNLCTRDPA 324
           ASVSMSFLAFVAFAFSSLISGYNLCTRDPA
Sbjct: 301 ASVSMSFLAFVAFAFSSLISGYNLCTRDPA 330

BLAST of Bhi02G000335 vs. TrEMBL
Match: tr|A0A0A0LNX3|A0A0A0LNX3_CUCSA (CASP-like protein OS=Cucumis sativus OX=3659 GN=Csa_1G004970 PE=3 SV=1)

HSP 1 Score: 499.6 bits (1285), Expect = 5.3e-138
Identity = 294/333 (88.29%), Postives = 296/333 (88.89%), Query Frame = 0

Query: 1   MIETKLKAQRSND-SSSMKKXXXXXXXXXVHSHMESPHSPLRFHSPLRSDAGDPPETXXX 60
           MIETKL+AQRSND SSS   XXXXXXXX VHSHMESPHSPLRFHSPLRSDAGDP ET   
Sbjct: 1   MIETKLRAQRSNDSSSSXXXXXXXXXXXSVHSHMESPHSPLRFHSPLRSDAGDPMETPPY 60

Query: 61  XXXXXXXXXXXXNSKAIVVVDVVDKSTQFSPLPSPYAGSRMPPENVNFTGDRSTSSKVMF 120
                       NSKAIVVVDVVDKSTQFSPLPSPYA SR P ENVNF GDRSTSSK+MF
Sbjct: 61  ASPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYAASRKPLENVNFVGDRSTSSKIMF 120

Query: 121 NRAMKEEVPQSVTKVGPA---------XXXXXXXXXXXXXXXXXXPVKQMAPMSRRSKDD 180
           NRAMKEEVPQSVTKVGP          XXXXXXXXXXXXXXXXXXPVKQMAPMSRRSKDD
Sbjct: 121 NRAMKEEVPQSVTKVGPVGVGXXXXXXXXXXXXXXXXXXXXXXXXPVKQMAPMSRRSKDD 180

Query: 181 ALTKAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQ 240
           AL KAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQ
Sbjct: 181 ALVKAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQ 240

Query: 241 AFDLVFTLIKKNHMIRHQFRCYFDFFMDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQ 300
           AFDLVFTLIKKNHMIRHQFRCYFDFFMDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQ
Sbjct: 241 AFDLVFTLIKKNHMIRHQFRCYFDFFMDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQ 300

Query: 301 LASASVSMSFLAFVAFAFSSLISGYNLCTRDPA 324
           LASASVSMSFLAFVAFA SSLISGYNLCTRDPA
Sbjct: 301 LASASVSMSFLAFVAFAVSSLISGYNLCTRDPA 333

BLAST of Bhi02G000335 vs. TrEMBL
Match: tr|M5WBA7|M5WBA7_PRUPE (CASP-like protein OS=Prunus persica OX=3760 GN=PRUPE_5G174700 PE=3 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 3.5e-81
Identity = 177/286 (61.89%), Postives = 210/286 (73.43%), Query Frame = 0

Query: 38  SPLRFHSPLRSDAGDPPETXXXXXXXXXXXXXXXNSKAIVVVDVVDKSTQFSPLPSPYAG 97
           SPLRFHSP+RSDAGDPPET   XXXXXXXX    NSKAI+    VDK TQ+SPL      
Sbjct: 33  SPLRFHSPIRSDAGDPPETPPYXXXXXXXXRRVDNSKAIM---AVDKFTQYSPL-----R 92

Query: 98  SRMPPENVNFTGDRSTSSKVMFNRAMKEEVPQSVTKVGPAXXXXXXXXXXXXXXXXXXPV 157
            + PPEN      ++ S  V++NR+M+E++  SV+KVGP                     
Sbjct: 93  PQKPPENA-----KAPSPVVVYNRSMREDMAPSVSKVGPVVGANGGEDVVNGVIGGGR-- 152

Query: 158 KQMAPMSRRSKDDALTKAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCL 217
            +    +R  + D +  AALGFRV EV +CLISFSVMA+DKTQGWSGDSFDRYKEYRYCL
Sbjct: 153 SRGITSTRPKRGDMVKTAALGFRVSEVVLCLISFSVMAADKTQGWSGDSFDRYKEYRYCL 212

Query: 218 TVNIIAFVYAAFQAFDLVFTLIKKNHMIRHQFRCYFDFFMDQVLAYLLISSSSSAATRVD 277
            VN+IAFVYAAFQA++L + L+ + ++IRH  R +FDFFMDQVLAYLLIS+SSSAATRVD
Sbjct: 213 AVNVIAFVYAAFQAYNLSYHLVTRKYVIRHHLRRHFDFFMDQVLAYLLISASSSAATRVD 272

Query: 278 DWQSNWGKDEFTQLASASVSMSFLAFVAFAFSSLISGYNLCTRDPA 324
           DWQSNWGKDEFT++A+ASVSM+FLAFVAFA SSLISGYNLCT D A
Sbjct: 273 DWQSNWGKDEFTEMATASVSMAFLAFVAFALSSLISGYNLCTHDSA 303

BLAST of Bhi02G000335 vs. TrEMBL
Match: tr|A0A2I4EPG3|A0A2I4EPG3_9ROSI (CASP-like protein OS=Juglans regia OX=51240 GN=LOC108991478 PE=3 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 6.6e-80
Identity = 168/296 (56.76%), Postives = 208/296 (70.27%), Query Frame = 0

Query: 31  SHMESPHSPLRFHSPLRSDAGDPPETXXXXXXXXXXXXXXXNSKAIVVVDVVDKSTQFSP 90
           +HM+SPHSPLRFH+P+ SD+GDP +T               NS A+V   + DK T+ SP
Sbjct: 37  THMDSPHSPLRFHTPMPSDSGDPHDTPPYESPENSPGKPPDNSMAMV---IFDKFTRNSP 96

Query: 91  LPSPYAGSRMPPE----NVNFTGDRSTSSKVMFNRAMKEEVPQSVTKVGPAXXXXXXXXX 150
           L SP  G + PPE    N N     S S  ++ NRA++EE P SV+KVGP          
Sbjct: 97  LTSPSDGQK-PPESASKNRNALAVDSPSPGIVVNRAIREEPPPSVSKVGPVGSGDGRRSG 156

Query: 151 XXXXXXXXXPVKQMAPMSRRSKDDA-LTKAALGFRVCEVAVCLISFSVMASDKTQGWSGD 210
                        +A + +RSK +  L +AALGFR+ E  +CLISF+VMA+DKTQGWSGD
Sbjct: 157 ------------TVASILKRSKREVMLRRAALGFRLSEFVLCLISFAVMAADKTQGWSGD 216

Query: 211 SFDRYKEYRYCLTVNIIAFVYAAFQAFDLVFTLIKKNHMIRHQFRCYFDFFMDQVLAYLL 270
           SFDRYKEYRYCL++N+IAFVY++FQ +DL + L+  N++IRH  R +F FFMDQVLAYLL
Sbjct: 217 SFDRYKEYRYCLSINVIAFVYSSFQVYDLTYHLVTGNYVIRHYLRRHFVFFMDQVLAYLL 276

Query: 271 ISSSSSAATRVDDWQSNWGKDEFTQLASASVSMSFLAFVAFAFSSLISGYNLCTRD 322
           ISSSSSAATRVDDWQSNWGKDEFT++A+ASVSM+FLAF AFA SSLISGYNLCTR+
Sbjct: 277 ISSSSSAATRVDDWQSNWGKDEFTEMATASVSMAFLAFAAFAISSLISGYNLCTRE 316

BLAST of Bhi02G000335 vs. TrEMBL
Match: tr|A0A2I4G2D9|A0A2I4G2D9_9ROSI (CASP-like protein OS=Juglans regia OX=51240 GN=LOC109004096 PE=3 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 1.8e-77
Identity = 173/321 (53.89%), Postives = 208/321 (64.80%), Query Frame = 0

Query: 5   KLKAQRSNDSSSMKKXXXXXXXXXVHSHMESPHSPLRFHSPLRSDAGDPPETXXXXXXXX 64
           K  + R++DSSS               +MESPHSPLRFHSP  S+ GD PET        
Sbjct: 54  KKSSSRNSDSSS--------------RNMESPHSPLRFHSPQLSELGDLPETPPYVSPEN 113

Query: 65  XXXXXXXNSKAIVVVDVVDKSTQFSPLPSPYAGSR-MPPENV----NFTGDRSTSSKVMF 124
                   SKA+V     D+ TQ+SPLPSP  G +   PEN     +     S S  ++F
Sbjct: 114 FPDKPTEKSKAMV---SFDRFTQYSPLPSPLGGQKQQQPENEGKNRSVQAADSPSPVMVF 173

Query: 125 NRAMKEEVPQSVTKVGPAXXXXXXXXXXXXXXXXXXPVKQMAPMSRRSKDDALTKAALGF 184
           NRAM+EE P SV+++GP                    V  +   S+R  ++   +AAL F
Sbjct: 174 NRAMREEPPPSVSRLGPG---------SGGHGRRSGTVASIPQTSKR--EEIQERAALVF 233

Query: 185 RVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFDLVFTLI 244
           RV E+ +CLISFSVMA+DKTQGWSGDSFDRYKEYRYCL+VN+IA VY+ FQA DL + + 
Sbjct: 234 RVSEIVLCLISFSVMAADKTQGWSGDSFDRYKEYRYCLSVNVIACVYSGFQASDLTYHMF 293

Query: 245 KKNHMIRHQFRCYFDFFMDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQLASASVSMS 304
              H IRH  R +FDFFMDQ+LAYLLISSSSSAATRVDDWQSNWGKDEFT++ASASV M+
Sbjct: 294 TGKHAIRHHLRRHFDFFMDQILAYLLISSSSSAATRVDDWQSNWGKDEFTEMASASVGMA 346

Query: 305 FLAFVAFAFSSLISGYNLCTR 321
           FLAF AFAFSSLISGY+LCTR
Sbjct: 354 FLAFAAFAFSSLISGYSLCTR 346

BLAST of Bhi02G000335 vs. NCBI nr
Match: XP_008453094.1 (PREDICTED: CASP-like protein 4A2 [Cucumis melo])

HSP 1 Score: 507.3 bits (1305), Expect = 3.9e-140
Identity = 298/330 (90.30%), Postives = 299/330 (90.61%), Query Frame = 0

Query: 1   MIETKLKAQRSND-SSSMKKXXXXXXXXXVHSHMESPHSPLRFHSPLRSDAGDPPETXXX 60
           MIETKL+AQRSND SSS   XXXXXXXX VHSHMESPHSPLRFHSPLRSDAGDP ET   
Sbjct: 1   MIETKLRAQRSNDSSSSXXXXXXXXXXXSVHSHMESPHSPLRFHSPLRSDAGDPMETPPY 60

Query: 61  XXXXXXXXXXXXNSKAIVVVDVVDKSTQFSPLPSPYAGSRMPPENVNFTGDRSTSSKVMF 120
                       NSKAIVVVDVVDKSTQFSPLPSPYAGSR P ENVNF GDRSTSSKVMF
Sbjct: 61  ASPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYAGSRKPLENVNFVGDRSTSSKVMF 120

Query: 121 NRAMKEEVPQSVTKVGPA------XXXXXXXXXXXXXXXXXXPVKQMAPMSRRSKDDALT 180
           NRAMKEEVPQSVTKVGPA      XXXXXXXXXXXXXXXXXXPVKQMAPMSRRSKDDAL 
Sbjct: 121 NRAMKEEVPQSVTKVGPAGVGGVXXXXXXXXXXXXXXXXXXXPVKQMAPMSRRSKDDALM 180

Query: 181 KAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFD 240
           KAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFD
Sbjct: 181 KAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFD 240

Query: 241 LVFTLIKKNHMIRHQFRCYFDFFMDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQLAS 300
           LVFTLIKKNHMIRHQFRCYFDFFMDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQLAS
Sbjct: 241 LVFTLIKKNHMIRHQFRCYFDFFMDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQLAS 300

Query: 301 ASVSMSFLAFVAFAFSSLISGYNLCTRDPA 324
           ASVSMSFLAFVAFAFSSLISGYNLCTRDPA
Sbjct: 301 ASVSMSFLAFVAFAFSSLISGYNLCTRDPA 330

BLAST of Bhi02G000335 vs. NCBI nr
Match: XP_004138112.2 (PREDICTED: CASP-like protein 4A2 [Cucumis sativus] >KGN63585.1 hypothetical protein Csa_1G004970 [Cucumis sativus])

HSP 1 Score: 499.6 bits (1285), Expect = 8.0e-138
Identity = 294/333 (88.29%), Postives = 296/333 (88.89%), Query Frame = 0

Query: 1   MIETKLKAQRSND-SSSMKKXXXXXXXXXVHSHMESPHSPLRFHSPLRSDAGDPPETXXX 60
           MIETKL+AQRSND SSS   XXXXXXXX VHSHMESPHSPLRFHSPLRSDAGDP ET   
Sbjct: 1   MIETKLRAQRSNDSSSSXXXXXXXXXXXSVHSHMESPHSPLRFHSPLRSDAGDPMETPPY 60

Query: 61  XXXXXXXXXXXXNSKAIVVVDVVDKSTQFSPLPSPYAGSRMPPENVNFTGDRSTSSKVMF 120
                       NSKAIVVVDVVDKSTQFSPLPSPYA SR P ENVNF GDRSTSSK+MF
Sbjct: 61  ASPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYAASRKPLENVNFVGDRSTSSKIMF 120

Query: 121 NRAMKEEVPQSVTKVGPA---------XXXXXXXXXXXXXXXXXXPVKQMAPMSRRSKDD 180
           NRAMKEEVPQSVTKVGP          XXXXXXXXXXXXXXXXXXPVKQMAPMSRRSKDD
Sbjct: 121 NRAMKEEVPQSVTKVGPVGVGXXXXXXXXXXXXXXXXXXXXXXXXPVKQMAPMSRRSKDD 180

Query: 181 ALTKAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQ 240
           AL KAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQ
Sbjct: 181 ALVKAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQ 240

Query: 241 AFDLVFTLIKKNHMIRHQFRCYFDFFMDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQ 300
           AFDLVFTLIKKNHMIRHQFRCYFDFFMDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQ
Sbjct: 241 AFDLVFTLIKKNHMIRHQFRCYFDFFMDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQ 300

Query: 301 LASASVSMSFLAFVAFAFSSLISGYNLCTRDPA 324
           LASASVSMSFLAFVAFA SSLISGYNLCTRDPA
Sbjct: 301 LASASVSMSFLAFVAFAVSSLISGYNLCTRDPA 333

BLAST of Bhi02G000335 vs. NCBI nr
Match: XP_022135555.1 (CASP-like protein 4A2 [Momordica charantia])

HSP 1 Score: 484.6 bits (1246), Expect = 2.7e-133
Identity = 291/324 (89.81%), Postives = 301/324 (92.90%), Query Frame = 0

Query: 1   MIETKLKAQRSNDSSSMKKXXXXXXXXXVHSHMESPHSPLRFHSPLRSDAGDPPETXXXX 60
           MIETKLK QRSNDSSSM+K XXXXXXXX+HSHMESPHSPLRFHSPLRSDAGDPPETXXXX
Sbjct: 1   MIETKLKTQRSNDSSSMRKSXXXXXXXXLHSHMESPHSPLRFHSPLRSDAGDPPETXXXX 60

Query: 61  XXXXXXXXXXXNSKAIVVVDVVDKSTQFSPLPSPYAGSRMPP-ENVNFTGDRSTSSKVMF 120
           XXXXXXXXXXXNSKAIVVVDVVDKSTQFSPLPSPYA SR PP EN NF  DRSTSSKVMF
Sbjct: 61  XXXXXXXXXXXNSKAIVVVDVVDKSTQFSPLPSPYADSRKPPAENANFPADRSTSSKVMF 120

Query: 121 NRAMKEEVPQSVTKVGPAXXXXXXXXXXXXXXXXXXPVKQMAPMSRRSKDDALTKAALGF 180
           NRAM+EEVPQSVTKVGPA      XXXXXXXXXXXX  KQMAP  RRSK DALTKAALGF
Sbjct: 121 NRAMREEVPQSVTKVGPA---ATGXXXXXXXXXXXXXXKQMAPAPRRSKADALTKAALGF 180

Query: 181 RVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFDLVFTLI 240
           RVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVN+IAF+YAAFQAFDLV+TLI
Sbjct: 181 RVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNVIAFIYAAFQAFDLVYTLI 240

Query: 241 KKNHMIRHQFRCYFDFFMDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQLASASVSMS 300
           KK HMIRH  R +FDFF+DQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQ+A+ASVSMS
Sbjct: 241 KKKHMIRHYLRYHFDFFVDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQMATASVSMS 300

Query: 301 FLAFVAFAFSSLISGYNLCTRDPA 324
           FLAFVAFAFSSLISGYNLCTRDPA
Sbjct: 301 FLAFVAFAFSSLISGYNLCTRDPA 321

BLAST of Bhi02G000335 vs. NCBI nr
Match: XP_022925737.1 (CASP-like protein 4A2 [Cucurbita moschata])

HSP 1 Score: 442.2 bits (1136), Expect = 1.5e-120
Identity = 266/323 (82.35%), Postives = 276/323 (85.45%), Query Frame = 0

Query: 1   MIETKLKAQRSNDSSSMKKXXXXXXXXXVHSHMESPHSPLRFHSPLRSDAGDPPETXXXX 60
           MIETKLKA+RS DSSSMKK         +HSHMESPHSPLRFHSPLRSD GDPPETXXXX
Sbjct: 1   MIETKLKAERSYDSSSMKKSSSRNSESSIHSHMESPHSPLRFHSPLRSDVGDPPETXXXX 60

Query: 61  XXXXXXXXXXXNSKAIVVVDVVDKSTQFSPLPSPYAGSRMPPENVNFTGDRSTSSKVMFN 120
           XXXXXXXXXXXNSK+IVVVDV +KSTQFSPL SP AGSR  PE  NF GDR  SSKVMFN
Sbjct: 61  XXXXXXXXXXXNSKSIVVVDVDNKSTQFSPLRSPNAGSRKLPEYANFPGDRLASSKVMFN 120

Query: 121 RAMKEEVPQSVTKVGPAXXXXXXXXXXXXXXXXXXPVKQMAPMSRRSKDDALTKAALGFR 180
           RAMKEEVPQSVTKVGPA      XXXXXXXXXXXX               ALTKAALGFR
Sbjct: 121 RAMKEEVPQSVTKVGPA----ASXXXXXXXXXXXXXXXXXXXXXXXXXXXALTKAALGFR 180

Query: 181 VCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFDLVFTLIK 240
           V EVAVCLISFSVMASDKT+GWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFDLV+TLIK
Sbjct: 181 VSEVAVCLISFSVMASDKTKGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFDLVYTLIK 240

Query: 241 KNHMIRHQFRCYFDFFMDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQLASASVSMSF 300
           K HMIRH+ R YFDFFMDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQ+ASAS+SMSF
Sbjct: 241 KQHMIRHRVRSYFDFFMDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQMASASISMSF 300

Query: 301 LAFVAFAFSSLISGYNLCTRDPA 324
           LAFVAFA SSLISGYNLC+RDPA
Sbjct: 301 LAFVAFAVSSLISGYNLCSRDPA 319

BLAST of Bhi02G000335 vs. NCBI nr
Match: XP_023530616.1 (CASP-like protein 4A2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 441.4 bits (1134), Expect = 2.6e-120
Identity = 269/323 (83.28%), Postives = 278/323 (86.07%), Query Frame = 0

Query: 1   MIETKLKAQRSNDSSSMKKXXXXXXXXXVHSHMESPHSPLRFHSPLRSDAGDPPETXXXX 60
           MIE KLKA+RS DSSSMKK     X   +HSHMESPHSPLRFHSPLRSD GDPPETXXXX
Sbjct: 1   MIEMKLKAERSYDSSSMKKSSSRNXESSIHSHMESPHSPLRFHSPLRSDVGDPPETXXXX 60

Query: 61  XXXXXXXXXXXNSKAIVVVDVVDKSTQFSPLPSPYAGSRMPPENVNFTGDRSTSSKVMFN 120
           XXXXXXXXXXX  K+IVVVDV +KSTQFSPL SP AGSR  PE  NF GDRSTSSKVMFN
Sbjct: 61  XXXXXXXXXXXXFKSIVVVDVDNKSTQFSPLRSPNAGSRKLPEYANFPGDRSTSSKVMFN 120

Query: 121 RAMKEEVPQSVTKVGPAXXXXXXXXXXXXXXXXXXPVKQMAPMSRRSKDDALTKAALGFR 180
           RAMKEEVPQSVTKVGPA    XXXXXXXXXXXXXX                LTKAALGFR
Sbjct: 121 RAMKEEVPQSVTKVGPA----XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLTKAALGFR 180

Query: 181 VCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFDLVFTLIK 240
           VCEVAVCLISFSVMASDKT+GWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFDLV+TLIK
Sbjct: 181 VCEVAVCLISFSVMASDKTKGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFDLVYTLIK 240

Query: 241 KNHMIRHQFRCYFDFFMDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQLASASVSMSF 300
           K HMIRH+ R YFDFFMDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQ+ASASVSMSF
Sbjct: 241 KQHMIRHRVRSYFDFFMDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQMASASVSMSF 300

Query: 301 LAFVAFAFSSLISGYNLCTRDPA 324
           LAFVAFA SSLISGYNLC+RDPA
Sbjct: 301 LAFVAFAVSSLISGYNLCSRDPA 319

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
sp|D7MMW4|CSPLJ_ARALL2.6e-6348.51CASP-like protein 4A2 OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALYDRAFT... [more]
sp|Q501G6|CSPLY_ARATH7.1e-6148.16CASP-like protein 4A2 OS=Arabidopsis thaliana OX=3702 GN=At5g62820 PE=2 SV=1[more]
sp|Q84WP5|CSPL8_ARATH4.0e-5668.18CASP-like protein 4A3 OS=Arabidopsis thaliana OX=3702 GN=At2g36330 PE=2 SV=1[more]
sp|D7LIR2|CSPLI_ARALL8.9e-5665.19CASP-like protein 4A3 OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALYDRAFT... [more]
sp|Q9FNE8|CSPLV_ARATH3.6e-4156.46CASP-like protein 4A1 OS=Arabidopsis thaliana OX=3702 GN=At5g40300 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT5G62820.13.9e-6248.16Uncharacterised protein family (UPF0497)[more]
AT2G36330.12.2e-5768.18Uncharacterised protein family (UPF0497)[more]
AT5G40300.12.0e-4256.46Uncharacterised protein family (UPF0497)[more]
AT2G38480.13.3e-1637.18Uncharacterised protein family (UPF0497)[more]
AT4G11655.16.8e-1435.42Uncharacterised protein family (UPF0497)[more]
Match NameE-valueIdentityDescription
tr|A0A1S3BWI9|A0A1S3BWI9_CUCME2.5e-14090.30CASP-like protein OS=Cucumis melo OX=3656 GN=LOC103493912 PE=3 SV=1[more]
tr|A0A0A0LNX3|A0A0A0LNX3_CUCSA5.3e-13888.29CASP-like protein OS=Cucumis sativus OX=3659 GN=Csa_1G004970 PE=3 SV=1[more]
tr|M5WBA7|M5WBA7_PRUPE3.5e-8161.89CASP-like protein OS=Prunus persica OX=3760 GN=PRUPE_5G174700 PE=3 SV=1[more]
tr|A0A2I4EPG3|A0A2I4EPG3_9ROSI6.6e-8056.76CASP-like protein OS=Juglans regia OX=51240 GN=LOC108991478 PE=3 SV=1[more]
tr|A0A2I4G2D9|A0A2I4G2D9_9ROSI1.8e-7753.89CASP-like protein OS=Juglans regia OX=51240 GN=LOC109004096 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_008453094.13.9e-14090.30PREDICTED: CASP-like protein 4A2 [Cucumis melo][more]
XP_004138112.28.0e-13888.29PREDICTED: CASP-like protein 4A2 [Cucumis sativus] >KGN63585.1 hypothetical prot... [more]
XP_022135555.12.7e-13389.81CASP-like protein 4A2 [Momordica charantia][more]
XP_022925737.11.5e-12082.35CASP-like protein 4A2 [Cucurbita moschata][more]
XP_023530616.12.6e-12083.28CASP-like protein 4A2 [Cucurbita pepo subsp. pepo][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR006702CASP_dom
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
cellular_component GO:0005575 cellular_component
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi02M000335Bhi02M000335mRNA


Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006702Casparian strip membrane protein domainPFAMPF04535DUF588coord: 170..305
e-value: 4.1E-32
score: 111.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 127..161
NoneNo IPR availablePANTHERPTHR11615:SF147CASP-LIKE PROTEIN 4A2-RELATEDcoord: 6..321
NoneNo IPR availablePANTHERPTHR11615NITRATE, FROMATE, IRON DEHYDROGENASEcoord: 6..321