Bhi02G000208 (gene) Wax gourd

NameBhi02G000208
Typegene
OrganismBenincasa hispida (Wax gourd)
DescriptionRibonuclease H superfamily polynucleotidyl transferase
Locationchr2 : 5649564 .. 5653869 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATAAATTTACGGGAGTTAATGATAATTCAAATTTTTTTTTTAATATAGTAGGATCAAACCTTTAATTTTTTTTTTTTTAAATGTCATGTCAATTATTGTTGAACAAATTTTAACAACTTAAAAGTAAAATTGGGCTTAATAAAAAAATATGAGCTTTAAATCTCAAAAAAAAAAGAAAAAAGAAAAAAAAGAGAAGAAAGTTTGCATAACCAAGTCTTGGATGATTTAAAAAGTTGAAAATGAGCATAATATGAATCTGGCCGATTATTCAATATAAACAACAACAACAATAATAATACTGATCATCATCGTCGTAAATTATTTTAGAGAGATTTTAAAAAATAGAAAATTTTAAAATATGGATTATTTAATTTAGAAATTGGAATGAGAATGAAGGGGATGATCCGTTGATTGGAAGTTCAGTGCAGGAAGCAAGCTCTTAAAGCCAGGGCGGTCGTTAACGTTATAATAATATCTTCACCTCTTCCCGCCTTTCTTCTTGTTCTTCTTGTTCTTCTTCTTCAATCTCGTATAAATCTACTAATCTTCTTTCTTTTCCCTCTGTTCTCTTCTTTGATTGAAAATTCCATTATGGGTTCAACGGAGGATCGATCGGAGATTGCCTTCTTTGACGTCGAGACCACCGTTCCCACCCGCCAAGGCCAGAGATTTTCAATTTTGGAATTTGGAGCCATTCTGGTGTGCCCTAGGAAGCTGGTTGAGTTGGAGAGCTATTCCACTCTCGTTCGACCTTCTGATCTCTCGCTCATTTCCTCCTTATCGGTGCGATGCAATGGCATTACTAGAGATGCCGTCATTTCTTCACCTACTTTCGCCCAAATTGCTGATAGGGTTTACGATATCCTTCACGGTGCTCACTCCTCTCTCTCTCTCTCTTTTGATTCCACACTAATTCTCTACTAGGGTATTTGAAAGGAAGCGGCGAAATGTTATCTATACATGTCCTTCTTTTTGATGTATTATGAATGTGGATTGATTGTTAAATTTATGTGGAAGTTGAATTGGGTGCCTTCTGATGGGACTGGGAGCTGGTTTCTGTTAAACAAGAGATTTTGGCCCAGAATTGTGTTCTTTTTTCTTCTGAACTTCAGACGACATTTCCTTAGTCAGGTTAGTTTAGTTGAAGGATGGTAACATTTTTAGGGACCCAAGTTTGTGTAAGGTTCGAAGAGACGAACATTGGTCGGGAGGCTTCTCAGACACTCTTCTTTGACTTCGCCCTTTTTTTCCCTACCAAATCTTTTTAGCAAGTGTGTATAGGTTTAAATTCAGATTACATTTCTCGGTTAGATGATCATTTCAGTTCTAAGATTGTACCATGCACTGTTGGGTTGAATGTTCCTGCTTCTTCTAGTTTAATTCTATTATATGCCTGTGTTTGAGTTTCCTCTGCTGCGATACTGTTTCCTGTAGGGCGGATATGGGCAGGCCATAACATACTCAGATTTGACTGTGCACGAATAAGGGAGGCTTTTGCAGAAATTGGGGTCCCAGCACCGGAGCCAAAAGGTACAATTGATTCATTGGCATTGTTGACTCAAAGGTTTGGAAGGAGAGCTGGCGACATGAAGGTAATGACAGAATTCTCTCTCTGTTGCATTCTTGCTATGTTTCTGCTTGTGTTCATGCAAAATTTCAGGGCTAGTCTCCATTAGACGGTACTTTTGTCAAATTTGTTTAGAGCTTTCAACATTCAGAACTCAAGCAGGAAATTGTAGAAGTAATTGAAGTTTTGGGATTTACCTCTCTAATTGAGACTTAAACCATGTTAACATTTGGCACTATGATAGGATTATATTACAAATGTAACATTTTACCAATTTAATATATCATGGTTAAATCTTATGCGAACAAGGGATGGAAAAACCTCTTTTTGGGGAAGATCTGTAATGATGAGGGATTAAGATTTGAAAAATTATGCATAAAGTGGTGAGAATAGGGTTCTTGTAATGATTTTTCTCTTCCTTGCGCTGAGTTTCTCTGGTAAATTCAACCCCATTGTCTTGGCTTTATATAGTTATCTTCATGCATATTCTGACTTTGGGTTTTGGTTTCTAGAAGTTATTGTAACTCTTTATTTATTTTATTTTATTTTTGTTTTTGACGTTCCTTTTCTCCCTGTAGATGGCTACTCTTGCTTCCTATTTTGGAATAGGACAACAAACACACAGGCAACCCTTCCAACTAGCTAGTAATTTTTTTAACTTAAATTAAAGGAGTGGTTCTAGTTATCTGACTTTGTCTGTGTAATTCTCCAGGAGTTTGGATGATGTCAGGATGAATCTTGAAGTTCTAAAGTATTGTGCAACTGTCTTGTTTCTGGTAATGTATAAATTTAAGATGTCGAACATAACTAATATGTTCATGTAAACTTGTAACTTTGTCTGTCTACAATTCATTAATTGTTTGGAGTTTAGACCATTGCCGATGATTACATGTATGGAATTTTTGTTGCATTATCTGAATATTGACCACTTGGATGTATGCCCGTGATAACCACAATTCTTTCTGAATTTAGATCATCAATAGGCTCCAAATGCTTCATTGTCAGTTTTTGCATCAATTGACAATACTTGTCTCCATTTTGCCTATCTGCCATGTGGAGACATTCTTCTTGTACTTTTTCTCTCTCTTTCTTTTTGAAGTAAAGTTTATTTGACAACAGCATAGAATTAGTTTTGCTCTTATAATGACCAGTTTATATATAATATAGTATGGTGGGTTAAGTTGAACTATTGTCTGGCTATAGGAATCAAGTCTACCCGAAGTTTTCCGAGAGAACAGTTGGGTTTCTCCAAATGCTGTTACTAGGCGCCGCACTGCAAAATCATCTCCACAGGGGAATAGTTCGAATAATGATACTCACTCATCAAGTTCAAGGATTGGTGGTAATCCCATATCTCTATTAGATCAAGGGGAAACTCATCCGATACTATCCCTCGTAACTTGCAGCTCTGAGGATGGAGCCTCAAATCTGGCTGAATCTAGAGCAACTGAATCAGATTCTTTTAACATGCGCACTCTCAGCGACCAAATAACTGGTGAATCGCTCGAAACAGATATCAGCATGGAAGAAGAACATGCCACAGTATCTACTGAAGCATCTCCCTCAGAGGATACTTCCACAAGTCTCAGCGCTACTACTGAATTTTTGGAGCCTGATCAGGTGTCTGTTTCATTCATCACTGCATCTTTCGTTCCATTTTTTCGTGGGAGCCAAAGAATACAATTATCGCACAAAGATGACTGTTTGCAGCTTCTTTGTAATAATCTGAGAGTTCGGTTTGGCATAAGCACAAAATTCACTGATTACGCTGGCCGTCCAAAGTTGAGTTTCGTGGTTGATGTACCACCAAGTTTATGTACGGTTCTTGAAGCATCTGATGGCGTGGCTCAGAGATTATTCTCTGATTCCGGGAGCGGGTCTGAATGGAGGCCTGTGGTGATGAGAAAGAATGATTATTTCAACTACCCGACAATGAGATTACAGTAAGTCATACTCCCATCTGATAGTTTGACATATTAGTTAACTACTATCTACAAATATTTTGATGAATTGAACTATCAAATGTGGGAAAAGGAAGAGAAAATAACAGAGAACATCTAGTCCAACGTATTATCCGCAAGCTTGCTTCTTTGCTTCAATCTTTGGTTTCTTGCCATTCATATCGATTCATCTGCTCTGTAAGTTCTTAATTTCCACTTCTTGGTCAGTTTCTAAAGGATATTAAATTTGTTTTTTCACAGCATTCCAACTGCAGTAAGTGGAGATGTGGCGATTTATGCTACAGAGATGTACCAAAAAGAATCATCAGGCGCTGCACAAAGGCTGATATTCAGTAAATTCGATGCTGCAGAGCTTGATAGCTTGATCAAACCTGGAGCCATCTTAGATGCATTCATTTCCTTGGATACATATGACTATCAACAGAGTGCAGGCATAAGATTGGTAGCTAAAAGGTTGATCATCCATTCCTAGTAATGGGTGATCAAGTTGTCCCATTATTGAAGTAGCAGTGAATACTGATGTTATGTTATATTATAGTTAGCTTGCTGACTAACATTCAAATTTCCAATTAGAATAATACTAAATTGATACAAGCTCCCGGTTCTTTGAAAGATCATTCAGTTATTTGTGTTTCTAATCAACTGTCTATACTACTTATGAACACATTAGACTTGATACTTTTCTAGAGACATTTCTGACACTTATAGGAGTGAAAAACATTTGAAATTTGTGGACAAACTATATTATTTTGGTTCTTGAAATAATATGTTTAGCAG

mRNA sequence

AATAAATTTACGGGAGTTAATGATAATTCAAATTTTTTTTTTAATATAGTAGGATCAAACCTTTAATTTTTTTTTTTTTAAATGTCATGTCAATTATTGTTGAACAAATTTTAACAACTTAAAAGTAAAATTGGGCTTAATAAAAAAATATGAGCTTTAAATCTCAAAAAAAAAAGAAAAAAGAAAAAAAAGAGAAGAAAGTTTGCATAACCAAGTCTTGGATGATTTAAAAAGTTGAAAATGAGCATAATATGAATCTGGCCGATTATTCAATATAAACAACAACAACAATAATAATACTGATCATCATCGTCGTAAATTATTTTAGAGAGATTTTAAAAAATAGAAAATTTTAAAATATGGATTATTTAATTTAGAAATTGGAATGAGAATGAAGGGGATGATCCGTTGATTGGAAGTTCAGTGCAGGAAGCAAGCTCTTAAAGCCAGGGCGGTCGTTAACGTTATAATAATATCTTCACCTCTTCCCGCCTTTCTTCTTGTTCTTCTTGTTCTTCTTCTTCAATCTCGTATAAATCTACTAATCTTCTTTCTTTTCCCTCTGTTCTCTTCTTTGATTGAAAATTCCATTATGGGTTCAACGGAGGATCGATCGGAGATTGCCTTCTTTGACGTCGAGACCACCGTTCCCACCCGCCAAGGCCAGAGATTTTCAATTTTGGAATTTGGAGCCATTCTGGTGTGCCCTAGGAAGCTGGTTGAGTTGGAGAGCTATTCCACTCTCGTTCGACCTTCTGATCTCTCGCTCATTTCCTCCTTATCGGTGCGATGCAATGGCATTACTAGAGATGCCGTCATTTCTTCACCTACTTTCGCCCAAATTGCTGATAGGGTTTACGATATCCTTCACGGGCGGATATGGGCAGGCCATAACATACTCAGATTTGACTGTGCACGAATAAGGGAGGCTTTTGCAGAAATTGGGGTCCCAGCACCGGAGCCAAAAGGTACAATTGATTCATTGGCATTGTTGACTCAAAGGTTTGGAAGGAGAGCTGGCGACATGAAGATGGCTACTCTTGCTTCCTATTTTGGAATAGGACAACAAACACACAGGAGTTTGGATGATGTCAGGATGAATCTTGAAGTTCTAAAGTATTGTGCAACTGTCTTGTTTCTGGAATCAAGTCTACCCGAAGTTTTCCGAGAGAACAGTTGGGTTTCTCCAAATGCTGTTACTAGGCGCCGCACTGCAAAATCATCTCCACAGGGGAATAGTTCGAATAATGATACTCACTCATCAAGTTCAAGGATTGGTGGTAATCCCATATCTCTATTAGATCAAGGGGAAACTCATCCGATACTATCCCTCGTAACTTGCAGCTCTGAGGATGGAGCCTCAAATCTGGCTGAATCTAGAGCAACTGAATCAGATTCTTTTAACATGCGCACTCTCAGCGACCAAATAACTGGTGAATCGCTCGAAACAGATATCAGCATGGAAGAAGAACATGCCACAGTATCTACTGAAGCATCTCCCTCAGAGGATACTTCCACAAGTCTCAGCGCTACTACTGAATTTTTGGAGCCTGATCAGGTGTCTGTTTCATTCATCACTGCATCTTTCGTTCCATTTTTTCGTGGGAGCCAAAGAATACAATTATCGCACAAAGATGACTGTTTGCAGCTTCTTTGTAATAATCTGAGAGTTCGGTTTGGCATAAGCACAAAATTCACTGATTACGCTGGCCGTCCAAAGTTGAGTTTCGTGGTTGATGTACCACCAAGTTTATGTACGGTTCTTGAAGCATCTGATGGCGTGGCTCAGAGATTATTCTCTGATTCCGGGAGCGGGTCTGAATGGAGGCCTGTGGTGATGAGAAAGAATGATTATTTCAACTACCCGACAATGAGATTACACATTCCAACTGCAGTAAGTGGAGATGTGGCGATTTATGCTACAGAGATGTACCAAAAAGAATCATCAGGCGCTGCACAAAGGCTGATATTCAGTAAATTCGATGCTGCAGAGCTTGATAGCTTGATCAAACCTGGAGCCATCTTAGATGCATTCATTTCCTTGGATACATATGACTATCAACAGAGTGCAGGCATAAGATTGGTAGCTAAAAGGTTGATCATCCATTCCTAGTAATGGGTGATCAAGTTGTCCCATTATTGAAGTAGCAGTGAATACTGATGTTATGTTATATTATAGTTAGCTTGCTGACTAACATTCAAATTTCCAATTAGAATAATACTAAATTGATACAAGCTCCCGGTTCTTTGAAAGATCATTCAGTTATTTGTGTTTCTAATCAACTGTCTATACTACTTATGAACACATTAGACTTGATACTTTTCTAGAGACATTTCTGACACTTATAGGAGTGAAAAACATTTGAAATTTGTGGACAAACTATATTATTTTGGTTCTTGAAATAATATGTTTAGCAG

Coding sequence (CDS)

ATGGGTTCAACGGAGGATCGATCGGAGATTGCCTTCTTTGACGTCGAGACCACCGTTCCCACCCGCCAAGGCCAGAGATTTTCAATTTTGGAATTTGGAGCCATTCTGGTGTGCCCTAGGAAGCTGGTTGAGTTGGAGAGCTATTCCACTCTCGTTCGACCTTCTGATCTCTCGCTCATTTCCTCCTTATCGGTGCGATGCAATGGCATTACTAGAGATGCCGTCATTTCTTCACCTACTTTCGCCCAAATTGCTGATAGGGTTTACGATATCCTTCACGGGCGGATATGGGCAGGCCATAACATACTCAGATTTGACTGTGCACGAATAAGGGAGGCTTTTGCAGAAATTGGGGTCCCAGCACCGGAGCCAAAAGGTACAATTGATTCATTGGCATTGTTGACTCAAAGGTTTGGAAGGAGAGCTGGCGACATGAAGATGGCTACTCTTGCTTCCTATTTTGGAATAGGACAACAAACACACAGGAGTTTGGATGATGTCAGGATGAATCTTGAAGTTCTAAAGTATTGTGCAACTGTCTTGTTTCTGGAATCAAGTCTACCCGAAGTTTTCCGAGAGAACAGTTGGGTTTCTCCAAATGCTGTTACTAGGCGCCGCACTGCAAAATCATCTCCACAGGGGAATAGTTCGAATAATGATACTCACTCATCAAGTTCAAGGATTGGTGGTAATCCCATATCTCTATTAGATCAAGGGGAAACTCATCCGATACTATCCCTCGTAACTTGCAGCTCTGAGGATGGAGCCTCAAATCTGGCTGAATCTAGAGCAACTGAATCAGATTCTTTTAACATGCGCACTCTCAGCGACCAAATAACTGGTGAATCGCTCGAAACAGATATCAGCATGGAAGAAGAACATGCCACAGTATCTACTGAAGCATCTCCCTCAGAGGATACTTCCACAAGTCTCAGCGCTACTACTGAATTTTTGGAGCCTGATCAGGTGTCTGTTTCATTCATCACTGCATCTTTCGTTCCATTTTTTCGTGGGAGCCAAAGAATACAATTATCGCACAAAGATGACTGTTTGCAGCTTCTTTGTAATAATCTGAGAGTTCGGTTTGGCATAAGCACAAAATTCACTGATTACGCTGGCCGTCCAAAGTTGAGTTTCGTGGTTGATGTACCACCAAGTTTATGTACGGTTCTTGAAGCATCTGATGGCGTGGCTCAGAGATTATTCTCTGATTCCGGGAGCGGGTCTGAATGGAGGCCTGTGGTGATGAGAAAGAATGATTATTTCAACTACCCGACAATGAGATTACACATTCCAACTGCAGTAAGTGGAGATGTGGCGATTTATGCTACAGAGATGTACCAAAAAGAATCATCAGGCGCTGCACAAAGGCTGATATTCAGTAAATTCGATGCTGCAGAGCTTGATAGCTTGATCAAACCTGGAGCCATCTTAGATGCATTCATTTCCTTGGATACATATGACTATCAACAGAGTGCAGGCATAAGATTGGTAGCTAAAAGGTTGATCATCCATTCCTAG

Protein sequence

MGSTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVPAPEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATVLFLESSLPEVFRENSWVSPNAVTRRRTAKSSPQGNSSNNDTHSSSSRIGGNPISLLDQGETHPILSLVTCSSEDGASNLAESRATESDSFNMRTLSDQITGESLETDISMEEEHATVSTEASPSEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQRIQLSHKDDCLQLLCNNLRVRFGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPVVMRKNDYFNYPTMRLHIPTAVSGDVAIYATEMYQKESSGAAQRLIFSKFDAAELDSLIKPGAILDAFISLDTYDYQQSAGIRLVAKRLIIHS
BLAST of Bhi02G000208 vs. Swiss-Prot
Match: sp|Q9FLR0|NEN1_ARATH (Protein NEN1 OS=Arabidopsis thaliana OX=3702 GN=NEN1 PE=2 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 2.4e-148
Identity = 293/505 (58.02%), Postives = 361/505 (71.49%), Query Frame = 0

Query: 5   EDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLS 64
           EDRSEIAFFDVETTVP R GQRF+ILEFG+ILVCP+KL EL SY+TLV+P+DLSLISSLS
Sbjct: 4   EDRSEIAFFDVETTVPKR-GQRFAILEFGSILVCPKKLTELRSYTTLVQPADLSLISSLS 63

Query: 65  VRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVPAPEP 124
           VRCNGI RD V+ +P FA IAD VYDILHGRIWAGHNILRFDCARIREAFAEIG   PEP
Sbjct: 64  VRCNGIKRDDVVLAPLFADIADTVYDILHGRIWAGHNILRFDCARIREAFAEIGRQPPEP 123

Query: 125 KGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATVLFLE 184
           KG IDSL LLTQ+FGRRAGDMKMATLA YFG+G QTHRSLDDVRMNLEVLKYCATVLFLE
Sbjct: 124 KGAIDSLGLLTQKFGRRAGDMKMATLARYFGLGNQTHRSLDDVRMNLEVLKYCATVLFLE 183

Query: 185 SSLPEVFRENSWVSPNAVTRRRTAKSSPQGNSSNNDTHSSSSRIGGNPISLLDQGETHPI 244
           SSLP    +NS VSP  ++ RR   +S +GN     T ++S R+                
Sbjct: 184 SSLPYAHVDNS-VSPETISSRRRIDASREGN-----TVTTSVRL---------------- 243

Query: 245 LSLVTCSSEDGASNLAESRATESDSFNMRTLSDQITGES-LETDISMEEEHATVSTEASP 304
                        +++E+ A + D FNM  L +++  ++ +++DI MEEE    S +   
Sbjct: 244 ------------PSISENSAAQPDPFNMSVLRNEMASDNHIQSDILMEEEQIEPS-DVVA 303

Query: 305 SEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQ--RIQLSHKDDCLQLLCNNLRVR 364
           SE+TS        FL PD +S+S I A   PF+ GSQ  +++L H D  LQL C+ L++R
Sbjct: 304 SENTSDH----EGFLTPDAMSLSNIKAMLFPFYPGSQMMKLKLLHGDSPLQLYCSYLKIR 363

Query: 365 FGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPVVMRKNDY 424
           FG++ KF D  GR +L+FVVD+ PSL ++LEA D  AQ+L  DSGS SEW PVV     +
Sbjct: 364 FGVNGKFLDNTGRRRLNFVVDLNPSLYSILEACDSNAQKLSVDSGSTSEWNPVVNPMKGF 423

Query: 425 FNYPTMRLHIPTAVSGDVAIYATEMYQKESSGAAQRLIFSKFDAAELDSLIKPGAILDAF 484
            NYP  R+HI T ++GD A YATE++Q+ESSGA Q+LIFS  +  EL+SL+  G+++DAF
Sbjct: 424 VNYPNARIHIATEINGDEARYATEIHQRESSGATQKLIFSNPNNEELESLLTTGSVVDAF 468

Query: 485 ISLDTYDYQQSAGIRLVAKRLIIHS 507
           +SL+ YDYQQ AGIRLVAK+L+I S
Sbjct: 484 LSLEPYDYQQKAGIRLVAKKLVIQS 468

BLAST of Bhi02G000208 vs. Swiss-Prot
Match: sp|Q9CA74|NEN3_ARATH (Protein NEN3 OS=Arabidopsis thaliana OX=3702 GN=NEN3 PE=2 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 5.7e-142
Identity = 273/510 (53.53%), Postives = 357/510 (70.00%), Query Frame = 0

Query: 5   EDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLS 64
           ++RSEIAFFD+ET VPT+ G+ F+ILEFGAILVCPR+L EL SYSTLVRP+DLSLIS+L+
Sbjct: 8   DERSEIAFFDLETAVPTKSGEPFAILEFGAILVCPRRLEELYSYSTLVRPTDLSLISTLT 67

Query: 65  VRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVPAPEP 124
            R +GITRD V+S+ TF++IAD+VYDILHGRIWAGHNI+RFDC RIREAFAEIG+  PEP
Sbjct: 68  KRRSGITRDGVLSAHTFSEIADKVYDILHGRIWAGHNIIRFDCVRIREAFAEIGLSPPEP 127

Query: 125 KGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATVLFLE 184
           K TIDSL+LL+Q+FG+RAGDMKMA+LA+YFG+G Q HRSLDDVRMNLEV+KYCATVLFLE
Sbjct: 128 KATIDSLSLLSQKFGKRAGDMKMASLATYFGLGDQAHRSLDDVRMNLEVVKYCATVLFLE 187

Query: 185 SSLPEVFRENSWVSPNAVTRRRT--------AKSSPQGNSSNNDTHSSSSRIGGNPISLL 244
           SS+P++  + SW SP    R R+         + S   +SS+  T  SSS +    +   
Sbjct: 188 SSVPDILTDMSWFSPRKSPRTRSNGKLVANGVRESSTSSSSSPKTDPSSSSVDATIV--- 247

Query: 245 DQGETHPILSLVT-CSSEDGASNLAESRATESDSFNMRTLSDQITGESLETDISMEEEHA 304
              + HPI+SL+T CS  D +S   E      D  ++ TL  ++   +L+TD + E +  
Sbjct: 248 ---KNHPIVSLLTECSESDASSYDIE------DPIDITTLIGKLRIGTLQTDAAEEAKTV 307

Query: 305 TVSTEASPSEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQRIQLSHKDDCLQLLC 364
               E+ PS D+    +    FL  ++VSVS I AS VPF+RGS R++L H D  L L  
Sbjct: 308 RQQDESPPSPDSD---AKDESFLGVNEVSVSSIRASLVPFYRGSLRMKLFHNDTPLHLCW 367

Query: 365 NNLRVRFGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPVV 424
           ++L+VRFGIS KF D+AGRPKL+ +VD P  LC +L+A D  A  L +DS + S+WRP V
Sbjct: 368 HSLKVRFGISRKFVDHAGRPKLNIIVDAPLDLCKILDAVDAAAHNLPTDSSTNSDWRPTV 427

Query: 425 MRKNDYFNYPTMRLHIPTAVSGDVAIYATEMYQKESS-GAAQRLIFSKFDAAELDSLIKP 484
           +RK  + NYPT RLHI +  +GD  +  T++YQKE   G  Q+L  S  +  +L+S + P
Sbjct: 428 IRKEGFANYPTARLHISSESNGDDTLCGTQVYQKEEPLGTNQKLDVSSDNLEKLESALLP 487

Query: 485 GAILDAFISLDTYDYQQSAGIRLVAKRLII 505
           G ++DAF SL+ Y YQQ AGIRL  K+L+I
Sbjct: 488 GTLVDAFFSLEPYSYQQMAGIRLAVKKLVI 502

BLAST of Bhi02G000208 vs. Swiss-Prot
Match: sp|Q0V842|NEN2_ARATH (Protein NEN2 OS=Arabidopsis thaliana OX=3702 GN=NEN2 PE=2 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 3.7e-141
Identity = 283/508 (55.71%), Postives = 351/508 (69.09%), Query Frame = 0

Query: 5   EDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLS 64
           EDRSEIAFFDVETT+P R GQ ++ILEFG+ILVCP+KLVEL++YS LVRP++L+LI+  S
Sbjct: 11  EDRSEIAFFDVETTIPFRVGQGYAILEFGSILVCPKKLVELKNYSVLVRPANLNLITPRS 70

Query: 65  VRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVPAPEP 124
           V+CNGI R+ V S+ TFA IAD VYDILHGRIWAGHNIL+FDC RIREAFAEIG   PEP
Sbjct: 71  VKCNGIKREDVESALTFADIADTVYDILHGRIWAGHNILKFDCPRIREAFAEIGRDPPEP 130

Query: 125 KGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATVLFLE 184
           KGTIDSLALLTQRFGRRAGDMKMATLA+YFG+G QTHRSLDDVRMN EVLKYCATVLFLE
Sbjct: 131 KGTIDSLALLTQRFGRRAGDMKMATLATYFGLGNQTHRSLDDVRMNFEVLKYCATVLFLE 190

Query: 185 SSLPEVFRENS---WVSPNAVTRRRTAKSSPQGNSSNNDTHSSSSRIGGNPISLLDQGET 244
           SSLP+   ENS                K SP  + ++  T  + + I             
Sbjct: 191 SSLPDELIENSVXXXXXXXXXXXXXXIKKSPLQSPTDQQTGENMTTI------------- 250

Query: 245 HPILSLVTCSSEDGASNLAESRATESDSFNMRTLSDQITGESLETDISMEEEHATVSTEA 304
            PILS V+            S   ++D F+M TL ++I  E L++D+ MEEE     +E 
Sbjct: 251 -PILSFVS------------SAEAQTDPFDMSTLRNEIAPEVLQSDVPMEEEQ-NQQSET 310

Query: 305 SPSEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQ--RIQLSHKDDCLQLLCNNLR 364
             SE T         F+E D++SVS I A+ VP + GSQ  ++QL   D  LQL C  L+
Sbjct: 311 VASEGTGDQ----EGFMELDKISVSSIRATHVPLYDGSQTMKLQLFLGDRPLQLHCPRLK 370

Query: 365 VRFGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPVVMRKN 424
           VRFGI+ KF D AGR +L+FV+D+ PSLC VL+  D  AQ +  DSGSGS+W P+V+   
Sbjct: 371 VRFGINGKFMDKAGRRRLNFVIDLYPSLCNVLQECDSAAQTISVDSGSGSDWNPLVIPMK 430

Query: 425 DYFNYPTMRLHIPTAVSGDVAIYATEMYQKESSG--AAQRLIFSKFDAAELDSLIKPGAI 484
            + N PT R+HIPT ++GD+  YA E++QKE SG  A Q+LI S   A E++SL+ P  +
Sbjct: 431 GFLNCPTARIHIPTELNGDIDRYAAEIHQKEFSGATATQKLISSNPKAEEIESLLNPRTV 487

Query: 485 LDAFISLDTYDYQQSAGIRLVAKRLIIH 506
           LDAF+SL+ YDYQQ AGIRLVA++L+IH
Sbjct: 491 LDAFLSLEPYDYQQRAGIRLVARKLVIH 487

BLAST of Bhi02G000208 vs. Swiss-Prot
Match: sp|F4JJ23|NEN4_ARATH (Protein NEN4 OS=Arabidopsis thaliana OX=3702 GN=NEN4 PE=2 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 5.0e-69
Identity = 125/199 (62.81%), Postives = 158/199 (79.40%), Query Frame = 0

Query: 8   SEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLSVRC 67
           +EI FFD+ET VP + GQ F ILEFGAI+VCP+KL ELES++TL++P DLS++S  S R 
Sbjct: 8   NEIVFFDLETNVPNKAGQHFHILEFGAIIVCPKKLEELESFTTLIQPKDLSVVSIRSSRS 67

Query: 68  NGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVPAPEPKGT 127
           +GITR  V ++P+F  +A++++ +L+GRIWAGHNI RFDC RI+EAFAEIG  APEP G 
Sbjct: 68  DGITRAKVTNAPSFEDVAEKIHGLLNGRIWAGHNIRRFDCVRIKEAFAEIGKAAPEPSGI 127

Query: 128 IDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATVLFLESSL 187
           IDSL LL+ +FG+RAG+MKMA+LA+YFG+G Q HRSLDDVRMNLEVLK+CATVLFLES+L
Sbjct: 128 IDSLGLLSDKFGKRAGNMKMASLAAYFGLGVQKHRSLDDVRMNLEVLKHCATVLFLESTL 187

Query: 188 PEVFRENSWVSPNAVTRRR 207
           P         S   +TR R
Sbjct: 188 PNHLEGKWHTSSKIMTRSR 206

BLAST of Bhi02G000208 vs. TAIR10
Match: AT5G07710.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein)

HSP 1 Score: 526.9 bits (1356), Expect = 1.3e-149
Identity = 293/505 (58.02%), Postives = 361/505 (71.49%), Query Frame = 0

Query: 5   EDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLS 64
           EDRSEIAFFDVETTVP R GQRF+ILEFG+ILVCP+KL EL SY+TLV+P+DLSLISSLS
Sbjct: 4   EDRSEIAFFDVETTVPKR-GQRFAILEFGSILVCPKKLTELRSYTTLVQPADLSLISSLS 63

Query: 65  VRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVPAPEP 124
           VRCNGI RD V+ +P FA IAD VYDILHGRIWAGHNILRFDCARIREAFAEIG   PEP
Sbjct: 64  VRCNGIKRDDVVLAPLFADIADTVYDILHGRIWAGHNILRFDCARIREAFAEIGRQPPEP 123

Query: 125 KGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATVLFLE 184
           KG IDSL LLTQ+FGRRAGDMKMATLA YFG+G QTHRSLDDVRMNLEVLKYCATVLFLE
Sbjct: 124 KGAIDSLGLLTQKFGRRAGDMKMATLARYFGLGNQTHRSLDDVRMNLEVLKYCATVLFLE 183

Query: 185 SSLPEVFRENSWVSPNAVTRRRTAKSSPQGNSSNNDTHSSSSRIGGNPISLLDQGETHPI 244
           SSLP    +NS VSP  ++ RR   +S +GN     T ++S R+                
Sbjct: 184 SSLPYAHVDNS-VSPETISSRRRIDASREGN-----TVTTSVRL---------------- 243

Query: 245 LSLVTCSSEDGASNLAESRATESDSFNMRTLSDQITGES-LETDISMEEEHATVSTEASP 304
                        +++E+ A + D FNM  L +++  ++ +++DI MEEE    S +   
Sbjct: 244 ------------PSISENSAAQPDPFNMSVLRNEMASDNHIQSDILMEEEQIEPS-DVVA 303

Query: 305 SEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQ--RIQLSHKDDCLQLLCNNLRVR 364
           SE+TS        FL PD +S+S I A   PF+ GSQ  +++L H D  LQL C+ L++R
Sbjct: 304 SENTSDH----EGFLTPDAMSLSNIKAMLFPFYPGSQMMKLKLLHGDSPLQLYCSYLKIR 363

Query: 365 FGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPVVMRKNDY 424
           FG++ KF D  GR +L+FVVD+ PSL ++LEA D  AQ+L  DSGS SEW PVV     +
Sbjct: 364 FGVNGKFLDNTGRRRLNFVVDLNPSLYSILEACDSNAQKLSVDSGSTSEWNPVVNPMKGF 423

Query: 425 FNYPTMRLHIPTAVSGDVAIYATEMYQKESSGAAQRLIFSKFDAAELDSLIKPGAILDAF 484
            NYP  R+HI T ++GD A YATE++Q+ESSGA Q+LIFS  +  EL+SL+  G+++DAF
Sbjct: 424 VNYPNARIHIATEINGDEARYATEIHQRESSGATQKLIFSNPNNEELESLLTTGSVVDAF 468

Query: 485 ISLDTYDYQQSAGIRLVAKRLIIHS 507
           +SL+ YDYQQ AGIRLVAK+L+I S
Sbjct: 484 LSLEPYDYQQKAGIRLVAKKLVIQS 468

BLAST of Bhi02G000208 vs. TAIR10
Match: AT1G74390.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein)

HSP 1 Score: 505.8 bits (1301), Expect = 3.2e-143
Identity = 273/510 (53.53%), Postives = 357/510 (70.00%), Query Frame = 0

Query: 5   EDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLS 64
           ++RSEIAFFD+ET VPT+ G+ F+ILEFGAILVCPR+L EL SYSTLVRP+DLSLIS+L+
Sbjct: 8   DERSEIAFFDLETAVPTKSGEPFAILEFGAILVCPRRLEELYSYSTLVRPTDLSLISTLT 67

Query: 65  VRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVPAPEP 124
            R +GITRD V+S+ TF++IAD+VYDILHGRIWAGHNI+RFDC RIREAFAEIG+  PEP
Sbjct: 68  KRRSGITRDGVLSAHTFSEIADKVYDILHGRIWAGHNIIRFDCVRIREAFAEIGLSPPEP 127

Query: 125 KGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATVLFLE 184
           K TIDSL+LL+Q+FG+RAGDMKMA+LA+YFG+G Q HRSLDDVRMNLEV+KYCATVLFLE
Sbjct: 128 KATIDSLSLLSQKFGKRAGDMKMASLATYFGLGDQAHRSLDDVRMNLEVVKYCATVLFLE 187

Query: 185 SSLPEVFRENSWVSPNAVTRRRT--------AKSSPQGNSSNNDTHSSSSRIGGNPISLL 244
           SS+P++  + SW SP    R R+         + S   +SS+  T  SSS +    +   
Sbjct: 188 SSVPDILTDMSWFSPRKSPRTRSNGKLVANGVRESSTSSSSSPKTDPSSSSVDATIV--- 247

Query: 245 DQGETHPILSLVT-CSSEDGASNLAESRATESDSFNMRTLSDQITGESLETDISMEEEHA 304
              + HPI+SL+T CS  D +S   E      D  ++ TL  ++   +L+TD + E +  
Sbjct: 248 ---KNHPIVSLLTECSESDASSYDIE------DPIDITTLIGKLRIGTLQTDAAEEAKTV 307

Query: 305 TVSTEASPSEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQRIQLSHKDDCLQLLC 364
               E+ PS D+    +    FL  ++VSVS I AS VPF+RGS R++L H D  L L  
Sbjct: 308 RQQDESPPSPDSD---AKDESFLGVNEVSVSSIRASLVPFYRGSLRMKLFHNDTPLHLCW 367

Query: 365 NNLRVRFGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPVV 424
           ++L+VRFGIS KF D+AGRPKL+ +VD P  LC +L+A D  A  L +DS + S+WRP V
Sbjct: 368 HSLKVRFGISRKFVDHAGRPKLNIIVDAPLDLCKILDAVDAAAHNLPTDSSTNSDWRPTV 427

Query: 425 MRKNDYFNYPTMRLHIPTAVSGDVAIYATEMYQKESS-GAAQRLIFSKFDAAELDSLIKP 484
           +RK  + NYPT RLHI +  +GD  +  T++YQKE   G  Q+L  S  +  +L+S + P
Sbjct: 428 IRKEGFANYPTARLHISSESNGDDTLCGTQVYQKEEPLGTNQKLDVSSDNLEKLESALLP 487

Query: 485 GAILDAFISLDTYDYQQSAGIRLVAKRLII 505
           G ++DAF SL+ Y YQQ AGIRL  K+L+I
Sbjct: 488 GTLVDAFFSLEPYSYQQMAGIRLAVKKLVI 502

BLAST of Bhi02G000208 vs. TAIR10
Match: AT5G61390.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein)

HSP 1 Score: 503.1 bits (1294), Expect = 2.1e-142
Identity = 283/508 (55.71%), Postives = 351/508 (69.09%), Query Frame = 0

Query: 5   EDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLS 64
           EDRSEIAFFDVETT+P R GQ ++ILEFG+ILVCP+KLVEL++YS LVRP++L+LI+  S
Sbjct: 11  EDRSEIAFFDVETTIPFRVGQGYAILEFGSILVCPKKLVELKNYSVLVRPANLNLITPRS 70

Query: 65  VRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVPAPEP 124
           V+CNGI R+ V S+ TFA IAD VYDILHGRIWAGHNIL+FDC RIREAFAEIG   PEP
Sbjct: 71  VKCNGIKREDVESALTFADIADTVYDILHGRIWAGHNILKFDCPRIREAFAEIGRDPPEP 130

Query: 125 KGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATVLFLE 184
           KGTIDSLALLTQRFGRRAGDMKMATLA+YFG+G QTHRSLDDVRMN EVLKYCATVLFLE
Sbjct: 131 KGTIDSLALLTQRFGRRAGDMKMATLATYFGLGNQTHRSLDDVRMNFEVLKYCATVLFLE 190

Query: 185 SSLPEVFRENS---WVSPNAVTRRRTAKSSPQGNSSNNDTHSSSSRIGGNPISLLDQGET 244
           SSLP+   ENS                K SP  + ++  T  + + I             
Sbjct: 191 SSLPDELIENSVXXXXXXXXXXXXXXIKKSPLQSPTDQQTGENMTTI------------- 250

Query: 245 HPILSLVTCSSEDGASNLAESRATESDSFNMRTLSDQITGESLETDISMEEEHATVSTEA 304
            PILS V+            S   ++D F+M TL ++I  E L++D+ MEEE     +E 
Sbjct: 251 -PILSFVS------------SAEAQTDPFDMSTLRNEIAPEVLQSDVPMEEEQ-NQQSET 310

Query: 305 SPSEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQ--RIQLSHKDDCLQLLCNNLR 364
             SE T         F+E D++SVS I A+ VP + GSQ  ++QL   D  LQL C  L+
Sbjct: 311 VASEGTGDQ----EGFMELDKISVSSIRATHVPLYDGSQTMKLQLFLGDRPLQLHCPRLK 370

Query: 365 VRFGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPVVMRKN 424
           VRFGI+ KF D AGR +L+FV+D+ PSLC VL+  D  AQ +  DSGSGS+W P+V+   
Sbjct: 371 VRFGINGKFMDKAGRRRLNFVIDLYPSLCNVLQECDSAAQTISVDSGSGSDWNPLVIPMK 430

Query: 425 DYFNYPTMRLHIPTAVSGDVAIYATEMYQKESSG--AAQRLIFSKFDAAELDSLIKPGAI 484
            + N PT R+HIPT ++GD+  YA E++QKE SG  A Q+LI S   A E++SL+ P  +
Sbjct: 431 GFLNCPTARIHIPTELNGDIDRYAAEIHQKEFSGATATQKLISSNPKAEEIESLLNPRTV 487

Query: 485 LDAFISLDTYDYQQSAGIRLVAKRLIIH 506
           LDAF+SL+ YDYQQ AGIRLVA++L+IH
Sbjct: 491 LDAFLSLEPYDYQQRAGIRLVARKLVIH 487

BLAST of Bhi02G000208 vs. TAIR10
Match: AT4G39810.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein)

HSP 1 Score: 263.5 bits (672), Expect = 2.8e-70
Identity = 125/199 (62.81%), Postives = 158/199 (79.40%), Query Frame = 0

Query: 8   SEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLSVRC 67
           +EI FFD+ET VP + GQ F ILEFGAI+VCP+KL ELES++TL++P DLS++S  S R 
Sbjct: 8   NEIVFFDLETNVPNKAGQHFHILEFGAIIVCPKKLEELESFTTLIQPKDLSVVSIRSSRS 67

Query: 68  NGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVPAPEPKGT 127
           +GITR  V ++P+F  +A++++ +L+GRIWAGHNI RFDC RI+EAFAEIG  APEP G 
Sbjct: 68  DGITRAKVTNAPSFEDVAEKIHGLLNGRIWAGHNIRRFDCVRIKEAFAEIGKAAPEPSGI 127

Query: 128 IDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATVLFLESSL 187
           IDSL LL+ +FG+RAG+MKMA+LA+YFG+G Q HRSLDDVRMNLEVLK+CATVLFLES+L
Sbjct: 128 IDSLGLLSDKFGKRAGNMKMASLAAYFGLGVQKHRSLDDVRMNLEVLKHCATVLFLESTL 187

Query: 188 PEVFRENSWVSPNAVTRRR 207
           P         S   +TR R
Sbjct: 188 PNHLEGKWHTSSKIMTRSR 206

BLAST of Bhi02G000208 vs. TrEMBL
Match: tr|A0A1S3BWV4|A0A1S3BWV4_CUCME (protein NEN1 OS=Cucumis melo OX=3656 GN=LOC103494007 PE=4 SV=1)

HSP 1 Score: 864.0 bits (2231), Expect = 1.7e-247
Identity = 451/507 (88.95%), Postives = 463/507 (91.32%), Query Frame = 0

Query: 1   MGSTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           MG TEDRSEIAFFDVETTVPTRQGQ+FSILEFGAILVCP+KLVELESYSTLV+PSDLSLI
Sbjct: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFA+IGVP
Sbjct: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFADIGVP 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFRENSWVSPNAVTRRRTAKSSPQGNSSNNDTHSSSSRIGGNPISLLD-QG 240
           LFLESSLPEVF ENSWVSPNAVT                        IGGNPISL D QG
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTXXXXXXXXXXXXXXXXXXXXXXXXIGGNPISLTDQQG 240

Query: 241 ETHPILSLVTCSSEDGASNLAESRATESDSFNMRTLSDQITGESLETDISMEEEHATVST 300
           E HPILSLVTCSSEDGA NLAE  ATESDSFNM T+SDQITG +LETDI+ME EHATVST
Sbjct: 241 EAHPILSLVTCSSEDGALNLAEPSATESDSFNMHTISDQITGATLETDINMEGEHATVST 300

Query: 301 EASPSEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQRIQLSHKDDCLQLLCNNLR 360
           EASPSEDTSTSL  TT+FLEPDQVSV FITASFVPFFRGSQRIQL HKDDCLQLLCNNLR
Sbjct: 301 EASPSEDTSTSLH-TTKFLEPDQVSVLFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLR 360

Query: 361 VRFGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPVVMRKN 420
           VRFGISTKFTDYAGRP+LSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRP V RKN
Sbjct: 361 VRFGISTKFTDYAGRPRLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKN 420

Query: 421 DYFNYPTMRLHIPTAVSGDVAIYATEMYQKESSGAAQRLIFSKFDAAELDSLIKPGAILD 480
            YFNYPTMRLHIPTAVSGDVA YATEMYQK++SGA QRLIFSKFDAAELDSLIKPGAILD
Sbjct: 421 GYFNYPTMRLHIPTAVSGDVANYATEMYQKDASGAVQRLIFSKFDAAELDSLIKPGAILD 480

Query: 481 AFISLDTYDYQQSAGIRLVAKRLIIHS 507
           AFISLDTYDYQQSAGIRLVAK+LIIHS
Sbjct: 481 AFISLDTYDYQQSAGIRLVAKKLIIHS 506

BLAST of Bhi02G000208 vs. TrEMBL
Match: tr|A0A0A0LSC3|A0A0A0LSC3_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G009910 PE=4 SV=1)

HSP 1 Score: 848.6 bits (2191), Expect = 7.3e-243
Identity = 448/507 (88.36%), Postives = 460/507 (90.73%), Query Frame = 0

Query: 1   MGSTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           MG TEDRSEIAFFDVETTVPTRQGQ+FSILEFGAILVCP+KLVELESYSTLV+PSDLSLI
Sbjct: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP
Sbjct: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFRENSWVSPNAVTRRRTAKSSPQGNSSNNDTHSSSSRIGGNPISL-LDQG 240
           LFLESSLPEVF ENSWVSPNAVT                        IGGNPISL + QG
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTXXXXXXXXXXXXXXXXXXXXXXXXIGGNPISLPVQQG 240

Query: 241 ETHPILSLVTCSSEDGASNLAESRATESDSFNMRTLSDQITGESLETDISMEEEHATVST 300
           ETHPILSLVT  SED AS LAE  ATESDSFNM T SDQITG +LETDI+M EEHATVST
Sbjct: 241 ETHPILSLVTICSEDRASILAEPSATESDSFNMHTTSDQITGATLETDINM-EEHATVST 300

Query: 301 EASPSEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQRIQLSHKDDCLQLLCNNLR 360
           EASPSEDTSTSL  TT+FLEPDQVSVSFITASFVPFFRGSQRIQL HKDDCLQLLCNNLR
Sbjct: 301 EASPSEDTSTSL-CTTKFLEPDQVSVSFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLR 360

Query: 361 VRFGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPVVMRKN 420
           VRFGISTKFTDYAGRP+LSFVVDVPP+LCTVLEASDGVAQRLFSDSGSGSEWRP V RKN
Sbjct: 361 VRFGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKN 420

Query: 421 DYFNYPTMRLHIPTAVSGDVAIYATEMYQKESSGAAQRLIFSKFDAAELDSLIKPGAILD 480
            YFNYPTMRLHIPTAVSGDVA YATEM+QKE+SGA QRLIFSKFDAAELDSLIKPGAILD
Sbjct: 421 GYFNYPTMRLHIPTAVSGDVANYATEMHQKEASGAVQRLIFSKFDAAELDSLIKPGAILD 480

Query: 481 AFISLDTYDYQQSAGIRLVAKRLIIHS 507
           AFISLDTYDYQQSAGIRLVAK+LII S
Sbjct: 481 AFISLDTYDYQQSAGIRLVAKKLIIRS 505

BLAST of Bhi02G000208 vs. TrEMBL
Match: tr|A0A1Q3BUU7|A0A1Q3BUU7_CEPFO (RNase_T domain-containing protein OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_15025 PE=4 SV=1)

HSP 1 Score: 659.4 bits (1700), Expect = 6.3e-186
Identity = 339/509 (66.60%), Postives = 405/509 (79.57%), Query Frame = 0

Query: 1   MGSTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           M  +E+RSEIAFFDVETTVPTR GQ F+ILEFG ILVCPRKL EL+SYSTL+RP DL+LI
Sbjct: 1   MDQSEERSEIAFFDVETTVPTRPGQGFAILEFGTILVCPRKLEELQSYSTLIRPGDLNLI 60

Query: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           S+LSVRCNGIT D+V S+P+F+QIA  V+DILHGRIWAGHNI+RFDCARIREAFAEIG+P
Sbjct: 61  SNLSVRCNGITPDSVSSAPSFSQIAPTVFDILHGRIWAGHNIVRFDCARIREAFAEIGMP 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           AP PKGTIDSLALLTQRFGRRAGDMKMA+LA+YFG+GQQTHRSLDDVRMNLEVLKYCATV
Sbjct: 121 APVPKGTIDSLALLTQRFGRRAGDMKMASLANYFGLGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFRENSWVSPNAVTRRRTAKSSPQGNSSNNDTHSSSSRIGGNPISLLDQ-- 240
           LFLESSLP +F + SWVSPNA TR R+   SP    +N  + ++S+    + I       
Sbjct: 181 LFLESSLPNIFTDKSWVSPNATTRSRSIVKSPPEGETNEISPATSTNAKNDSILFPTNER 240

Query: 241 -GETHPILSLVTCSSEDGASNLAESRATESDSFNMRTLSDQITGESLETDISMEEEHATV 300
            GE HPILSL+T SS +  ++L  S +  +DSF+M  L D++  ES + D+ M+E+    
Sbjct: 241 TGENHPILSLITHSSGEEVTDLDSSTSVGTDSFDMSPLRDEVNSESFQYDVHMDEKPEQE 300

Query: 301 STEASPSEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQRIQLSHKDDCLQLLCNN 360
           S++ SP+   S   S +  FL+PDQVS+  I AS VP F G QRIQL H+  CLQL C  
Sbjct: 301 SSKMSPAAAGSDGCSGSAGFLDPDQVSIPSIKASLVPLFPGIQRIQLLHEGVCLQLYCAC 360

Query: 361 LRVRFGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPVVMR 420
           LRVRFG+STKF D+AGRP+LSFVVD  PSLC VL+A + VAQ+L  DSGS S+WR VV R
Sbjct: 361 LRVRFGLSTKFVDHAGRPRLSFVVDTFPSLCKVLDACNSVAQKLSVDSGSSSDWRAVVSR 420

Query: 421 KNDYFNYPTMRLHIPTAVSGDVAIYATEMYQKESSGAAQRLIFSKFDAAELDSLIKPGAI 480
           K  YF+YPT R+HIPTAV+GD+A YATE+YQKE+SG AQ+L+FSKFDAAELD+L KPG  
Sbjct: 421 KPGYFSYPTARIHIPTAVNGDIAQYATEIYQKEASGTAQKLVFSKFDAAELDALFKPGIS 480

Query: 481 LDAFISLDTYDYQQSAGIRLVAKRLIIHS 507
           +DAF+SLD+YDYQQSAGIRLVAK+LIIHS
Sbjct: 481 VDAFLSLDSYDYQQSAGIRLVAKKLIIHS 509

BLAST of Bhi02G000208 vs. TrEMBL
Match: tr|A0A2P5DAP7|A0A2P5DAP7_9ROSA (Exonuclease/helicase-like OS=Trema orientalis OX=63057 GN=TorRG33x02_257180 PE=4 SV=1)

HSP 1 Score: 659.1 bits (1699), Expect = 8.2e-186
Identity = 346/511 (67.71%), Postives = 409/511 (80.04%), Query Frame = 0

Query: 1   MGSTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           MGS+EDR EIAFFDVETTVPTR G  F+ILEFG+ILVCPRKLVELESYSTLVRP+DLSLI
Sbjct: 1   MGSSEDRYEIAFFDVETTVPTRPGIGFAILEFGSILVCPRKLVELESYSTLVRPADLSLI 60

Query: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           S+LSVRCNGI+RDAV+++PTF  IADRVYDILHGRIWAGHNILRFDCARIREAFA+IG P
Sbjct: 61  STLSVRCNGISRDAVVAAPTFRDIADRVYDILHGRIWAGHNILRFDCARIREAFADIGRP 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           APEPKGTIDSLALLTQRFGRRAGDMKMATLA+YFG+GQQTHRSLDDVRMNLEVLKYCATV
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLATYFGLGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFRENSWVSPNAVTRRRT--AKSSPQGNSSNNDTHSSSSRIGGNP-ISLLD 240
           LFLESSLP++   NSWVSPNA+TR R+   KSSP+G S + +  S+S   G +  +S  +
Sbjct: 181 LFLESSLPDILTTNSWVSPNAITRSRSINGKSSPEGPSLSVNAPSASLNNGNSQVVSSTN 240

Query: 241 Q--GETHPILSLVTCSSEDGASNLAESRATESDSFNMRTLSDQITGESLETDISMEEEHA 300
           Q   E HPI SL+    E+   NL  S     D FNM  LS +I  ES  TD++MEE+  
Sbjct: 241 QITIENHPISSLMAPGREE-VPNLVGSNTPRPDPFNMAPLSSEIVIESDPTDVNMEEKSI 300

Query: 301 TVSTEASPSEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQRIQLSHKDDCLQLLC 360
            VS +   +       +++ EFLEPD VS+  I+AS  P + GSQ+I+L HKD+ LQL C
Sbjct: 301 PVSPDLLSTAAVYQCNNSSVEFLEPDDVSIPSISASLAPLYPGSQKIKLLHKDNSLQLQC 360

Query: 361 NNLRVRFGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPVV 420
             L+VRFGIS+KF D+AGRP+L+FVV   PSLC VL++ DG+AQ+L  +SGS SEW PVV
Sbjct: 361 ARLKVRFGISSKFVDHAGRPRLNFVVYASPSLCRVLDSCDGIAQKLSLESGSSSEWWPVV 420

Query: 421 MRKNDYFNYPTMRLHIPTAVSGDVAIYATEMYQKESSGAAQRLIFSKFDAAELDSLIKPG 480
            RK  + NYPT+RLHIPTAV G++AIYATE+YQKE+SG+ QRL+FSKFDA+EL+SL  PG
Sbjct: 421 TRKTGFVNYPTVRLHIPTAVCGEIAIYATEIYQKENSGSVQRLVFSKFDASELNSLFTPG 480

Query: 481 AILDAFISLDTYDYQQSAGIRLVAKRLIIHS 507
             +DAF SLDTYDYQQ+AGIRLVAK+LIIHS
Sbjct: 481 TFVDAFFSLDTYDYQQNAGIRLVAKKLIIHS 510

BLAST of Bhi02G000208 vs. TrEMBL
Match: tr|A0A067ENL7|A0A067ENL7_CITSI (Uncharacterized protein OS=Citrus sinensis OX=2711 GN=CISIN_1g010297mg PE=4 SV=1)

HSP 1 Score: 658.7 bits (1698), Expect = 1.1e-185
Identity = 350/512 (68.36%), Postives = 406/512 (79.30%), Query Frame = 0

Query: 1   MGSTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           MG T+DRSEIAFFDVETTVPTR GQRF+ILEFGAILVCP+KL EL +YSTLVRP+D  LI
Sbjct: 1   MGPTQDRSEIAFFDVETTVPTRPGQRFAILEFGAILVCPKKLEELHNYSTLVRPADPELI 60

Query: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           SSLSVRCNGIT DAV+SSPTFA IAD V+DILHGRIWAGHNILRFDCARIREAFAEIG P
Sbjct: 61  SSLSVRCNGITPDAVVSSPTFADIADTVFDILHGRIWAGHNILRFDCARIREAFAEIGRP 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           APEPKGTIDSLALLTQRFGRRAGDMKMA+LA+YFG+GQQTHRSLDDVRMNLEVLKYCATV
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKMASLATYFGLGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFRENSWVSPNAVTRRRT-AKSSPQGNSSNNDTHSSSSRI-GGNPISLLDQ 240
           LFLESSLP++F  NSWVSPNA TR R+ AKSSP+G S N  T SS  +    +  S  DQ
Sbjct: 181 LFLESSLPDIFTVNSWVSPNATTRSRSNAKSSPEGTSQNLSTPSSKFKFENASNASPPDQ 240

Query: 241 G--ETHPILSLVTCSSEDGASNLAESRATESDSFNMRTLSDQITGESLETDISMEEEHAT 300
           G  E HPI SLVT  + +  S LA + A + + F+M  LS+Q+  +SL+ D +ME++ A 
Sbjct: 241 GKEEIHPIFSLVTQGTAEVDSVLAVTTADQPNPFDMSQLSNQMETQSLQPDDTMEDKSAP 300

Query: 301 VSTEASPSEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGS--QRIQLSHKDDCLQLL 360
            S E   +   S   S    F+EPD+VS+S I+   VP F GS  Q+I+L H D  LQL 
Sbjct: 301 ESREMCSTASVSEGSSGYAGFMEPDEVSLSSISDGVVPLFPGSQIQKIKLLHNDVILQLC 360

Query: 361 CNNLRVRFGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPV 420
           C+ L++RFGISTKF D AGRP+LSFVVD+  SLCTVL+A + + ++L  DSGS SEWR V
Sbjct: 361 CSRLKIRFGISTKFVDQAGRPRLSFVVDISQSLCTVLDACEVIVRKLVEDSGSSSEWRHV 420

Query: 421 VMRKNDYFNYPTMRLHIPTAVSGDVAIYATEMYQKESSGAAQRLIFSKFDAAELDSLIKP 480
           V RK  +FN+PT RLHIPT V G+VA YATEMYQKE+SG  Q+L+F KFDAAELDSL+KP
Sbjct: 421 VTRKGGFFNHPTARLHIPTVVFGNVARYATEMYQKEASGTIQKLVFVKFDAAELDSLLKP 480

Query: 481 GAILDAFISLDTYDYQQSAGIRLVAKRLIIHS 507
           G  +DAF+SLD YDYQQSAGIRLVAK+LIIHS
Sbjct: 481 GTFVDAFLSLDPYDYQQSAGIRLVAKKLIIHS 512

BLAST of Bhi02G000208 vs. NCBI nr
Match: XP_022921575.1 (protein NEN1-like [Cucurbita moschata])

HSP 1 Score: 879.4 bits (2271), Expect = 5.8e-252
Identity = 459/522 (87.93%), Postives = 479/522 (91.76%), Query Frame = 0

Query: 1   MGSTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           MG T DRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKL ELESYSTLVRPSDLSLI
Sbjct: 1   MGPTGDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLDELESYSTLVRPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           SSLSVRCNGITRDAVIS+PTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIG+P
Sbjct: 61  SSLSVRCNGITRDAVISAPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           AP+PKGTIDSLALLTQ+FGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV
Sbjct: 121 APQPKGTIDSLALLTQKFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFRENSWVSPNAVTRRRTAKSSPQGNSSN--------------NDTHSSSS 240
           LFLESSLPEVF ENSWVSPNAVTRRR AKSSPQG ++N                     S
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGTNANXXXXXXXXXXXXXXXXXXXXXS 240

Query: 241 RIGGNPISLLD-QGETHPILSLVTCSSEDGASNL-AESRATESDSFNMRTLSDQITGESL 300
           RIGG+PISL D QGE HPILSLVTCSSEDGASNL A+S ATESDSFNM TLSDQI GESL
Sbjct: 241 RIGGSPISLTDQQGEAHPILSLVTCSSEDGASNLVADSDATESDSFNMGTLSDQIAGESL 300

Query: 301 ETDISMEEEHATVSTEASPSEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQRIQL 360
           ETD SMEEEHA+VSTEASP+E+TSTS+S+ TEFLEPDQVSVS ITASFVPFFRGSQRIQL
Sbjct: 301 ETDTSMEEEHASVSTEASPTENTSTSISSATEFLEPDQVSVSSITASFVPFFRGSQRIQL 360

Query: 361 SHKDDCLQLLCNNLRVRFGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSD 420
           SHKDDCLQLLCNNLRVRFGISTKFTDYAGRP+L+FVVDVPPSLC VLEASDGVAQRLFS+
Sbjct: 361 SHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLNFVVDVPPSLCRVLEASDGVAQRLFSE 420

Query: 421 SGSGSEWRPVVMRKNDYFNYPTMRLHIPTAVSGDVAIYATEMYQKESSGAAQRLIFSKFD 480
           SGSGSEWRPVVMRKN YFNYPTMRLHIPTAVSGD+AIYATEMYQKE+SG AQRLIFSKFD
Sbjct: 421 SGSGSEWRPVVMRKNGYFNYPTMRLHIPTAVSGDMAIYATEMYQKEASGGAQRLIFSKFD 480

Query: 481 AAELDSLIKPGAILDAFISLDTYDYQQSAGIRLVAKRLIIHS 507
           A ELDSLI PG I+DAFISLDTYDYQQSAGIRLVAK+LIIHS
Sbjct: 481 AVELDSLINPGGIIDAFISLDTYDYQQSAGIRLVAKKLIIHS 522

BLAST of Bhi02G000208 vs. NCBI nr
Match: XP_023515370.1 (protein NEN1-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 874.4 bits (2258), Expect = 1.9e-250
Identity = 459/522 (87.93%), Postives = 476/522 (91.19%), Query Frame = 0

Query: 1   MGSTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           MG T DRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKL ELESYSTLVRPSDLSLI
Sbjct: 1   MGPTGDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLDELESYSTLVRPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           SSLSVRCNGITRDAVIS+PTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIG+P
Sbjct: 61  SSLSVRCNGITRDAVISAPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           APEPKGTIDSLALLTQ+FGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV
Sbjct: 121 APEPKGTIDSLALLTQKFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFRENSWVSPNAVTRRRTAKSSPQGNSSN--------------NDTHSSSS 240
           LFLESSLPEVF ENSW+SPNAVTRRR AKSSPQG ++N                      
Sbjct: 181 LFLESSLPEVFPENSWISPNAVTRRRAAKSSPQGINANXXXXXXXXXXXXXXXXXXXXXX 240

Query: 241 RIGGNPISLLD-QGETHPILSLVTCSSEDGASNL-AESRATESDSFNMRTLSDQITGESL 300
           RIGG+PISL D QGE HPILSLVTCSSEDGASNL A+S ATESDSFNM TLSDQI GESL
Sbjct: 241 RIGGSPISLTDQQGEAHPILSLVTCSSEDGASNLVADSDATESDSFNMGTLSDQIAGESL 300

Query: 301 ETDISMEEEHATVSTEASPSEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQRIQL 360
           ET  SMEEEHA VSTEASP+E+TSTSLSA TEFLEPDQVSVS ITASFVPFFRGSQRIQL
Sbjct: 301 ETYSSMEEEHAPVSTEASPTENTSTSLSAATEFLEPDQVSVSSITASFVPFFRGSQRIQL 360

Query: 361 SHKDDCLQLLCNNLRVRFGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSD 420
           SHKDDCLQLLCNNLRVRFGISTKFTDYAGRP+L+FVVDVPPSLC VLEASDGVAQRLFS+
Sbjct: 361 SHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLNFVVDVPPSLCRVLEASDGVAQRLFSE 420

Query: 421 SGSGSEWRPVVMRKNDYFNYPTMRLHIPTAVSGDVAIYATEMYQKESSGAAQRLIFSKFD 480
           SGSGSEWRPVVMRKN YFNYPTMRLHIPTAVSGD+AIYATEMYQKE+SG AQRLIFSKFD
Sbjct: 421 SGSGSEWRPVVMRKNGYFNYPTMRLHIPTAVSGDMAIYATEMYQKEASGGAQRLIFSKFD 480

Query: 481 AAELDSLIKPGAILDAFISLDTYDYQQSAGIRLVAKRLIIHS 507
           A ELDSLI PG I+DAFISLDTYDYQQSAGIRLVAK+LIIHS
Sbjct: 481 AVELDSLINPGGIIDAFISLDTYDYQQSAGIRLVAKKLIIHS 522

BLAST of Bhi02G000208 vs. NCBI nr
Match: XP_022987423.1 (protein NEN1-like [Cucurbita maxima])

HSP 1 Score: 872.8 bits (2254), Expect = 5.5e-250
Identity = 458/522 (87.74%), Postives = 474/522 (90.80%), Query Frame = 0

Query: 1   MGSTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           MG T DRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKL ELESYSTLVRPSDLSLI
Sbjct: 1   MGPTGDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLDELESYSTLVRPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           SSLSVRCNGITRDAVIS+PTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIG+P
Sbjct: 61  SSLSVRCNGITRDAVISAPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           AP+PKGTIDSLALLTQ+FGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV
Sbjct: 121 APQPKGTIDSLALLTQKFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFRENSWVSPNAVTRRRTAKSSPQGNSSN--------------NDTHSSSS 240
           LFLESSLPEVF ENSWVSPNAVTRRR AKSSPQG + N                      
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGINENXXXXXXXXXXXXXXXXXXXXXX 240

Query: 241 RIGGNPISLLD-QGETHPILSLVTCSSEDGASNL-AESRATESDSFNMRTLSDQITGESL 300
            IGG+PISL D QGE HPILSLVTCSSEDGASNL A+S ATESDSFNM TLSDQI GESL
Sbjct: 241 XIGGSPISLTDQQGEAHPILSLVTCSSEDGASNLVADSDATESDSFNMGTLSDQIAGESL 300

Query: 301 ETDISMEEEHATVSTEASPSEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQRIQL 360
           ETD SMEEEHA VSTEASP+E+TSTSLSA TEFLEPDQVSVS ITASFVPFFRGSQRIQL
Sbjct: 301 ETDTSMEEEHAPVSTEASPTENTSTSLSAATEFLEPDQVSVSSITASFVPFFRGSQRIQL 360

Query: 361 SHKDDCLQLLCNNLRVRFGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSD 420
           SHKDDCLQLLCNNLRVRFGISTKFTDYAGRP+L+FVVDVPPSLC VLEASDGVAQRLFS+
Sbjct: 361 SHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLNFVVDVPPSLCRVLEASDGVAQRLFSE 420

Query: 421 SGSGSEWRPVVMRKNDYFNYPTMRLHIPTAVSGDVAIYATEMYQKESSGAAQRLIFSKFD 480
           SGSGSEWRPVVMRKN YFNYPTMRLHIPTAV GD+AIYATEMYQKE+SG AQRLIFSKFD
Sbjct: 421 SGSGSEWRPVVMRKNGYFNYPTMRLHIPTAVCGDMAIYATEMYQKEASGGAQRLIFSKFD 480

Query: 481 AAELDSLIKPGAILDAFISLDTYDYQQSAGIRLVAKRLIIHS 507
           A ELDSLI PG I+DAFISLDTYDYQQSAGIRLVAK+LIIHS
Sbjct: 481 AVELDSLINPGGIIDAFISLDTYDYQQSAGIRLVAKKLIIHS 522

BLAST of Bhi02G000208 vs. NCBI nr
Match: XP_023515369.1 (protein NEN1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 869.0 bits (2244), Expect = 7.9e-249
Identity = 459/536 (85.63%), Postives = 476/536 (88.81%), Query Frame = 0

Query: 1   MGSTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           MG T DRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKL ELESYSTLVRPSDLSLI
Sbjct: 1   MGPTGDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLDELESYSTLVRPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           SSLSVRCNGITRDAVIS+PTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIG+P
Sbjct: 61  SSLSVRCNGITRDAVISAPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           APEPKGTIDSLALLTQ+FGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV
Sbjct: 121 APEPKGTIDSLALLTQKFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFRENSWVSPNAVTRRRTAKSSPQGNSSN---------------------- 240
           LFLESSLPEVF ENSW+SPNAVTRRR AKSSPQG ++N                      
Sbjct: 181 LFLESSLPEVFPENSWISPNAVTRRRAAKSSPQGINANXXXXXXXXXXXXXXXXXXXXXX 240

Query: 241 ------NDTHSSSSRIGGNPISLLD-QGETHPILSLVTCSSEDGASNL-AESRATESDSF 300
                         RIGG+PISL D QGE HPILSLVTCSSEDGASNL A+S ATESDSF
Sbjct: 241 XXXXXXXXXXXXXXRIGGSPISLTDQQGEAHPILSLVTCSSEDGASNLVADSDATESDSF 300

Query: 301 NMRTLSDQITGESLETDISMEEEHATVSTEASPSEDTSTSLSATTEFLEPDQVSVSFITA 360
           NM TLSDQI GESLET  SMEEEHA VSTEASP+E+TSTSLSA TEFLEPDQVSVS ITA
Sbjct: 301 NMGTLSDQIAGESLETYSSMEEEHAPVSTEASPTENTSTSLSAATEFLEPDQVSVSSITA 360

Query: 361 SFVPFFRGSQRIQLSHKDDCLQLLCNNLRVRFGISTKFTDYAGRPKLSFVVDVPPSLCTV 420
           SFVPFFRGSQRIQLSHKDDCLQLLCNNLRVRFGISTKFTDYAGRP+L+FVVDVPPSLC V
Sbjct: 361 SFVPFFRGSQRIQLSHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLNFVVDVPPSLCRV 420

Query: 421 LEASDGVAQRLFSDSGSGSEWRPVVMRKNDYFNYPTMRLHIPTAVSGDVAIYATEMYQKE 480
           LEASDGVAQRLFS+SGSGSEWRPVVMRKN YFNYPTMRLHIPTAVSGD+AIYATEMYQKE
Sbjct: 421 LEASDGVAQRLFSESGSGSEWRPVVMRKNGYFNYPTMRLHIPTAVSGDMAIYATEMYQKE 480

Query: 481 SSGAAQRLIFSKFDAAELDSLIKPGAILDAFISLDTYDYQQSAGIRLVAKRLIIHS 507
           +SG AQRLIFSKFDA ELDSLI PG I+DAFISLDTYDYQQSAGIRLVAK+LIIHS
Sbjct: 481 ASGGAQRLIFSKFDAVELDSLINPGGIIDAFISLDTYDYQQSAGIRLVAKKLIIHS 536

BLAST of Bhi02G000208 vs. NCBI nr
Match: XP_008453220.1 (PREDICTED: protein NEN1 [Cucumis melo])

HSP 1 Score: 864.0 bits (2231), Expect = 2.5e-247
Identity = 451/507 (88.95%), Postives = 463/507 (91.32%), Query Frame = 0

Query: 1   MGSTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           MG TEDRSEIAFFDVETTVPTRQGQ+FSILEFGAILVCP+KLVELESYSTLV+PSDLSLI
Sbjct: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFA+IGVP
Sbjct: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFADIGVP 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFRENSWVSPNAVTRRRTAKSSPQGNSSNNDTHSSSSRIGGNPISLLD-QG 240
           LFLESSLPEVF ENSWVSPNAVT                        IGGNPISL D QG
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTXXXXXXXXXXXXXXXXXXXXXXXXIGGNPISLTDQQG 240

Query: 241 ETHPILSLVTCSSEDGASNLAESRATESDSFNMRTLSDQITGESLETDISMEEEHATVST 300
           E HPILSLVTCSSEDGA NLAE  ATESDSFNM T+SDQITG +LETDI+ME EHATVST
Sbjct: 241 EAHPILSLVTCSSEDGALNLAEPSATESDSFNMHTISDQITGATLETDINMEGEHATVST 300

Query: 301 EASPSEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQRIQLSHKDDCLQLLCNNLR 360
           EASPSEDTSTSL  TT+FLEPDQVSV FITASFVPFFRGSQRIQL HKDDCLQLLCNNLR
Sbjct: 301 EASPSEDTSTSLH-TTKFLEPDQVSVLFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLR 360

Query: 361 VRFGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPVVMRKN 420
           VRFGISTKFTDYAGRP+LSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRP V RKN
Sbjct: 361 VRFGISTKFTDYAGRPRLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKN 420

Query: 421 DYFNYPTMRLHIPTAVSGDVAIYATEMYQKESSGAAQRLIFSKFDAAELDSLIKPGAILD 480
            YFNYPTMRLHIPTAVSGDVA YATEMYQK++SGA QRLIFSKFDAAELDSLIKPGAILD
Sbjct: 421 GYFNYPTMRLHIPTAVSGDVANYATEMYQKDASGAVQRLIFSKFDAAELDSLIKPGAILD 480

Query: 481 AFISLDTYDYQQSAGIRLVAKRLIIHS 507
           AFISLDTYDYQQSAGIRLVAK+LIIHS
Sbjct: 481 AFISLDTYDYQQSAGIRLVAKKLIIHS 506

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
sp|Q9FLR0|NEN1_ARATH2.4e-14858.02Protein NEN1 OS=Arabidopsis thaliana OX=3702 GN=NEN1 PE=2 SV=1[more]
sp|Q9CA74|NEN3_ARATH5.7e-14253.53Protein NEN3 OS=Arabidopsis thaliana OX=3702 GN=NEN3 PE=2 SV=1[more]
sp|Q0V842|NEN2_ARATH3.7e-14155.71Protein NEN2 OS=Arabidopsis thaliana OX=3702 GN=NEN2 PE=2 SV=1[more]
sp|F4JJ23|NEN4_ARATH5.0e-6962.81Protein NEN4 OS=Arabidopsis thaliana OX=3702 GN=NEN4 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT5G07710.11.3e-14958.02Polynucleotidyl transferase, ribonuclease H-like superfamily protein[more]
AT1G74390.13.2e-14353.53Polynucleotidyl transferase, ribonuclease H-like superfamily protein[more]
AT5G61390.12.1e-14255.71Polynucleotidyl transferase, ribonuclease H-like superfamily protein[more]
AT4G39810.12.8e-7062.81Polynucleotidyl transferase, ribonuclease H-like superfamily protein[more]
Match NameE-valueIdentityDescription
tr|A0A1S3BWV4|A0A1S3BWV4_CUCME1.7e-24788.95protein NEN1 OS=Cucumis melo OX=3656 GN=LOC103494007 PE=4 SV=1[more]
tr|A0A0A0LSC3|A0A0A0LSC3_CUCSA7.3e-24388.36Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G009910 PE=4 SV=1[more]
tr|A0A1Q3BUU7|A0A1Q3BUU7_CEPFO6.3e-18666.60RNase_T domain-containing protein OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_... [more]
tr|A0A2P5DAP7|A0A2P5DAP7_9ROSA8.2e-18667.71Exonuclease/helicase-like OS=Trema orientalis OX=63057 GN=TorRG33x02_257180 PE=4... [more]
tr|A0A067ENL7|A0A067ENL7_CITSI1.1e-18568.36Uncharacterized protein OS=Citrus sinensis OX=2711 GN=CISIN_1g010297mg PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_022921575.15.8e-25287.93protein NEN1-like [Cucurbita moschata][more]
XP_023515370.11.9e-25087.93protein NEN1-like isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_022987423.15.5e-25087.74protein NEN1-like [Cucurbita maxima][more]
XP_023515369.17.9e-24985.63protein NEN1-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_008453220.12.5e-24788.95PREDICTED: protein NEN1 [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0003676nucleic acid binding
Vocabulary: INTERPRO
TermDefinition
IPR012337RNaseH-like_sf
IPR036397RNaseH_sf
IPR013520Exonuclease_RNaseT/DNA_pol3
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:0000160 phosphorelay signal transduction system
biological_process GO:0050789 regulation of biological process
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0008408 3'-5' exonuclease activity
molecular_function GO:0004386 helicase activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004527 exonuclease activity
molecular_function GO:0005515 protein binding
molecular_function GO:0003824 catalytic activity
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi02M000208Bhi02M000208mRNA


Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013520Exonuclease, RNase T/DNA polymerase IIISMARTSM00479exoiiienduscoord: 9..183
e-value: 4.2E-32
score: 122.6
IPR013520Exonuclease, RNase T/DNA polymerase IIIPFAMPF00929RNase_Tcoord: 12..174
e-value: 1.5E-23
score: 84.1
IPR036397Ribonuclease H superfamilyGENE3DG3DSA:3.30.420.10coord: 4..180
e-value: 1.4E-31
score: 111.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 295..311
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 292..311
NoneNo IPR availablePANTHERPTHR30231DNA POLYMERASE III SUBUNIT EPSILONcoord: 3..506
NoneNo IPR availablePANTHERPTHR30231:SF6PROTEIN NEN1-RELATEDcoord: 3..506
NoneNo IPR availableCDDcd06127DEDDhcoord: 11..175
e-value: 3.29494E-28
score: 108.927
IPR012337Ribonuclease H-like superfamilySUPERFAMILYSSF53098Ribonuclease H-likecoord: 9..179

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Bhi02G000208Wax gourdwgowgoB121
Bhi02G000208Wax gourdwgowgoB125
Bhi02G000208Cucumber (Gy14) v1cgywgoB188
Bhi02G000208Cucumber (Gy14) v1cgywgoB585
Bhi02G000208Cucumber (Gy14) v2cgybwgoB107
Bhi02G000208Cucurbita maxima (Rimu)cmawgoB0631
Bhi02G000208Cucurbita maxima (Rimu)cmawgoB0916
Bhi02G000208Cucurbita moschata (Rifu)cmowgoB0631
Bhi02G000208Cucurbita pepo (Zucchini)cpewgoB0737
Bhi02G000208Cucurbita moschata (Rifu)cmowgoB0917
Bhi02G000208Cucurbita pepo (Zucchini)cpewgoB0062
Bhi02G000208Cucurbita pepo (Zucchini)cpewgoB0581
Bhi02G000208Wild cucumber (PI 183967)cpiwgoB116
Bhi02G000208Wild cucumber (PI 183967)cpiwgoB528
Bhi02G000208Cucumber (Chinese Long) v3cucwgoB121
Bhi02G000208Cucumber (Chinese Long) v3cucwgoB125
Bhi02G000208Cucumber (Chinese Long) v3cucwgoB537
Bhi02G000208Cucumber (Chinese Long) v2cuwgoB115
Bhi02G000208Cucumber (Chinese Long) v2cuwgoB525
Bhi02G000208Bottle gourd (USVL1VR-Ls)lsiwgoB291
Bhi02G000208Bottle gourd (USVL1VR-Ls)lsiwgoB408
Bhi02G000208Melon (DHL92) v3.6.1medwgoB090
Bhi02G000208Melon (DHL92) v3.6.1medwgoB194
Bhi02G000208Melon (DHL92) v3.5.1mewgoB089
Bhi02G000208Melon (DHL92) v3.5.1mewgoB192
Bhi02G000208Watermelon (Charleston Gray)wcgwgoB407
Bhi02G000208Watermelon (Charleston Gray)wcgwgoB487
Bhi02G000208Watermelon (97103) v2wgowmbB644
Bhi02G000208Watermelon (97103) v2wgowmbB647
Bhi02G000208Watermelon (97103) v1wgowmB671
Bhi02G000208Watermelon (97103) v1wgowmB666
Bhi02G000208Silver-seed gourdcarwgoB0063