Bhi02G000142 (gene) Wax gourd

BLAST of Bhi02G000142 vs. Swiss-Prot
Match: sp|Q6H501|HKT6_ORYSJ (Probable cation transporter HKT6 OS=Oryza sativa subsp. japonica OX=39947 GN=HKT6 PE=2 SV=2)

HSP 1 Score: 411.0 bits (1055), Expect = 1.9e-113
Identity = 236/521 (45.30%), Postives = 333/521 (63.92%), Query Frame = 0

Query: 2   SSTKIIMLRIQRLSCFLIWMNPFWIQLLYFLFISSFGFFILVVLNPKTTSNNSFNPKKLD 61
           S TK     I+    F+   NP ++ + YF  IS  G+  L VL P+  SN     K LD
Sbjct: 25  SFTKSAQKSIKYSFQFIYQNNPLFVHVAYFALISFAGYGSLKVLKPRDKSNTL---KDLD 84

Query: 62  LFFTSVSASTVSSMSTVEMEVFSNSQLIVLTILMFIGGEVFTSMVELQLRKFKLKFSE-- 121
           + FTSVSASTVSSM+TVEME FS++QL VLTILM IGGEVFTSM+ +   + +    E  
Sbjct: 85  VLFTSVSASTVSSMATVEMEDFSSAQLWVLTILMLIGGEVFTSMLGIHFMRAEFGTKESV 144

Query: 122 -----QKIACVSSDLSVELGIVRNNNTLDIII----------LKYNSIKFLGFVVLGYLL 181
                     + S  S + G       + +            ++  +IKFLGFVV+GYLL
Sbjct: 145 STRDHSPCIDIESITSTKFGPSTQGTKVTVSFSELRMENGGHVEPKTIKFLGFVVMGYLL 204

Query: 182 IFNFLGIGMVLIYFKFVSSARQVLEQKGLKLSTFSIFLIVSTFASCGFVPTNENMIVFKK 241
           I N  G  ++ +Y   V SA ++L++KG+ +  FS+F  +S+  +CGF P NENMI+F+K
Sbjct: 205 ITNLGGSLLIYLYLNLVPSAHKILKRKGIGIIVFSVFTAISSVGNCGFTPVNENMIIFQK 264

Query: 242 NLGLLIILIPQALIGNTLFPSCLRICIWSLGKFMKKKKEIVGFLLKNSEEIGYLHLLPKL 301
           N  LL++++PQ L GNTLF  CLR+ +WSL K   KK     ++L+  + IGY HL+   
Sbjct: 265 NSILLLLILPQILAGNTLFAPCLRLMVWSLEKITGKKD--CRYILEYPKAIGYKHLMSTR 324

Query: 302 HSLLLVGSVLGFVLMQFVAIGAMEWRSNALSGLNCVEKLIGILFLSVNSRHSGESIVDLS 361
            S+ L  +V+  +++Q V   ++EW S AL G++  +K++  LF SVN+RH+GES+ DLS
Sbjct: 325 ESVYLTLTVVSLIILQTVLFLSLEWSSVALDGMSNYQKIVSALFQSVNARHAGESVTDLS 384

Query: 362 RLSSAILLLFVAMMYLPPYTSFMPIKIDEGEEEENQLLEANKRSRKIKRAKFMDNFVFSQ 421
            LSSAIL+L+  MMYLP YTSF+P    +GE+ + + +         KR   ++N++FS 
Sbjct: 385 NLSSAILVLYTIMMYLPGYTSFLP--RHDGEDSKTEKIN--------KRKGLLENWIFSH 444

Query: 422 LFYLIIFIFIICITERHNIKEDPLNFNIFNIIFEVISAYGNVGFTMGYNCKRQIHPQSNC 481
           + YL IF+ +ICITER ++  DPLNFN+F+I+FEV+SAYGNVGF++GY+CKR ++  + C
Sbjct: 445 MSYLAIFVMLICITERDSMATDPLNFNVFSILFEVVSAYGNVGFSVGYSCKRLLNHDARC 504

Query: 482 ADKSFGFSGKWSDNGKVVLIVVMIFGRLKKFNMNKQRPWKL 506
            D S+GF+GKWSDNGK +LI+VM+FGRLK FNM   R WKL
Sbjct: 505 KDASYGFAGKWSDNGKAILIIVMLFGRLKTFNMKGGRAWKL 530

BLAST of Bhi02G000142 vs. Swiss-Prot
Match: sp|Q84TI7|HKT1_ARATH (Sodium transporter HKT1 OS=Arabidopsis thaliana OX=3702 GN=HKT1 PE=1 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 1.7e-109
Identity = 235/471 (49.89%), Postives = 311/471 (66.03%), Query Frame = 0

Query: 44  VLNPKTTSNNSFNPKKLDLFFTSVSASTVSSMSTVEMEVFSNSQLIVLTILMFIGGEVFT 103
           +  P+TTS     P   DLFFTSVSA TVSSMSTV+MEVFSN+QLI LTILMF+GGE+FT
Sbjct: 42  ITKPRTTS----RPHDFDLFFTSVSAITVSSMSTVDMEVFSNTQLIFLTILMFLGGEIFT 101

Query: 104 SMVELQLRKF-KLKFSEQKIACV----SSDLSVELGIVRNNNT---LDIIILKYNSIKFL 163
           S + L +  F K  F   KI  +    +SD S+E        T     +I +   + K L
Sbjct: 102 SFLNLYVSYFTKFVFPHNKIRHILGSYNSDSSIEDRCDVETVTDYREGLIKIDERASKCL 161

Query: 164 GFVVLGYLLIFNFLGIGMVLIYFKFVSSARQVLEQKGLKLSTFSIFLIVSTFASCGFVPT 223
             VVL Y L+ N +G  ++L+Y  FV +AR VL  K +   TFS+F  VSTFA+CGFVPT
Sbjct: 162 YSVVLSYHLVTNLVGSVLLLVYVNFVKTARDVLSSKEISPLTFSVFTTVSTFANCGFVPT 221

Query: 224 NENMIVFKKNLGLLIILIPQALIGNTLFPSCLRICIWSLGKFMKKKKEIVGFLLKNSEEI 283
           NENMI+F+KN GL+ +LIPQ L+GNTLFP  L + IW L K  K+ +   G++LKN  ++
Sbjct: 222 NENMIIFRKNSGLIWLLIPQVLMGNTLFPCFLVLLIWGLYKITKRDE--YGYILKNHNKM 281

Query: 284 GYLHLLPKLHSLLLVGSVLGFVLMQFVAIGAMEWRSNALSGLNCVEKLIGILFLSVNSRH 343
           GY HLL     +LL  +VLGF+++Q +   A EW S +L G++  EKL+G LF  VNSRH
Sbjct: 282 GYSHLLSVRLCVLLGVTVLGFLIIQLLFFCAFEWTSESLEGMSSYEKLVGSLFQVVNSRH 341

Query: 344 SGESIVDLSRLSSAILLLFVAMMYLPPYTSFMPIKIDEGEEEENQLLEANKRSRKIKRAK 403
           +GE+IVDLS LS AIL+LF+ MMYLPPYT FMP+   +  E+E    + ++  +K+K++ 
Sbjct: 342 TGETIVDLSTLSPAILVLFILMMYLPPYTLFMPLTEQKTIEKEGG-DDDSENGKKVKKS- 401

Query: 404 FMDNFVFSQLFYLIIFIFIICITERHNIKEDPLNFNIFNIIFEVISAYGNVGFTMGYNCK 463
                + SQL +L I IF+I ITER N++ DP+NFN+ NI  EVISAYGNVGFT GY+C+
Sbjct: 402 ---GLIVSQLSFLTICIFLISITERQNLQRDPINFNVLNITLEVISAYGNVGFTTGYSCE 461

Query: 464 RQIH-PQSNCADKSFGFSGKWSDNGKVVLIVVMIFGRLKKFNMNKQRPWKL 506
           R++      C D S+GF+G+WS  GK VLI+VM +GR K+F     R W L
Sbjct: 462 RRVDISDGGCKDASYGFAGRWSPMGKFVLIIVMFYGRFKQFTAKSGRAWIL 501

BLAST of Bhi02G000142 vs. Swiss-Prot
Match: sp|Q7XPF7|HKT7_ORYSJ (Probable cation transporter HKT7 OS=Oryza sativa subsp. japonica OX=39947 GN=HKT7 PE=2 SV=2)

HSP 1 Score: 366.7 bits (940), Expect = 4.2e-100
Identity = 220/499 (44.09%), Postives = 311/499 (62.32%), Query Frame = 0

Query: 26  IQLLYFLFISSFGFFILVVLNPKTTSNNSFNPKKLDLFFTSVSASTVSSMSTVEMEVFSN 85
           + + YFL IS  G+ +L VL  +        P+++D FFT+VSA+TVSSMSTVEMEVFSN
Sbjct: 15  MHVAYFLAISCLGYGLLGVLKVR---EPGAAPRRIDRFFTAVSAATVSSMSTVEMEVFSN 74

Query: 86  SQLIVLTILMFIGGEVFTSMVELQLRKFKLKF-SEQKIACVSSDLSVEL-----GIVRN- 145
            QL+VLT+LM +GGEVF S+V L  +  KL+  +  +   V S   V L     G V N 
Sbjct: 75  GQLVVLTVLMLLGGEVFVSLVGLASKWSKLRSDAMDRSRRVESHGDVALADIDGGDVENP 134

Query: 146 ----------NNTLDIIILKYNSIKFLGFVVLGYLLIFNFLGIGMVLIYFKFVSSARQVL 205
                        +D   L++N+++ L ++VL    + + +G   V  Y      AR+ L
Sbjct: 135 TSSGEEAASRRRPMDADTLRHNAVRALFYIVLAIFAVVHVVGAVAVAAYVLASPGARRTL 194

Query: 206 EQKGLKLSTFSIFLIVSTFASCGFVPTNENMIVFKKNLGLLIILIPQALIGNTLFPSCLR 265
             K L   TF++F  VSTF++CGF+PTNENM+VFK++  L ++L+PQ L GNTLF   L 
Sbjct: 195 GDKSLNTWTFAVFTTVSTFSNCGFMPTNENMVVFKRDAPLQLLLVPQVLAGNTLFAPLLA 254

Query: 266 ICIWSLGKFMKKKKEIVGFLLK--NSEEIGYLHLLPKLHSLLLVGSVLGFVLMQFVAIGA 325
            C+W+      +++E+V    +   +   GY HL+P     +L  +V  FV +    +  
Sbjct: 255 ACVWAAAA-ATRREELVEMAREGGRAAAAGYAHLMPARRCWMLAATVAAFVAVLMALVCG 314

Query: 326 MEWRSNALSGLNCVEKLIGILFLSVNSRHSGESIVDLSRLSSAILLLFVAMMYLPPYTSF 385
           MEW   AL G++  EK++  LFL+VN+RH+GES VDLS L+ AIL+LFV MMYLPPYT++
Sbjct: 315 MEW-GGALQGMSPWEKVVNALFLAVNARHTGESTVDLSILAPAILVLFVLMMYLPPYTTW 374

Query: 386 MPIKIDEGEEEENQLLEANKRSRKIKRAKFMDNFVFSQLFYLIIFIFIICITERHNIKED 445
            P       EE +   ++N  ++ I   + +++ + SQL YL IF+  ICITER  +KED
Sbjct: 375 FPF------EENSTTKDSNAENQGI---RLLESTLLSQLSYLTIFVIAICITERRKLKED 434

Query: 446 PLNFNIFNIIFEVISAYGNVGFTMGYNCKRQIHPQSNCADKSFGFSGKWSDNGKVVLIVV 505
           PLNF++ +I+ EV+SAYGNVGF+MGY+C RQI+P   C DK  GF G+WSD+GK++LI V
Sbjct: 435 PLNFSVLSIVVEVVSAYGNVGFSMGYSCSRQINPDHLCTDKWTGFVGRWSDSGKLILIFV 494

BLAST of Bhi02G000142 vs. Swiss-Prot
Match: sp|Q93XI5|HKT2_ORYSI (Cation transporter HKT2 OS=Oryza sativa subsp. indica OX=39946 GN=HKT2 PE=1 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 5.4e-100
Identity = 218/510 (42.75%), Postives = 314/510 (61.57%), Query Frame = 0

Query: 21  MNPFWIQLLYFLFISSFGFFILVVLNPKTTSNNSFNPKKLDLFFTSVSASTVSSMSTVEM 80
           ++PFWIQL YFL IS  G  +L+ L P   S+  F P  +D+ F S SA TVS +ST+EM
Sbjct: 38  VHPFWIQLSYFLLISILGSVLLMFLKP---SSPEFKPGYIDMLFLSTSAMTVSGLSTIEM 97

Query: 81  EVFSNSQLIVLTILMFIGGEVFTSMVELQLR---KFKLKFSEQKIACVSSDLSVELGIVR 140
           EV S+SQ++VLT+LM +GGEVF S + L LR   K   +FS  ++    S + +EL  + 
Sbjct: 98  EVLSSSQIVVLTLLMLVGGEVFVSFLGLMLRLKHKHNPEFSGDRV----SSVPIELDTIE 157

Query: 141 NNNTL-------------DIIILKYNSIK-------FLGFVVLGYLLIFNFLGIGMVLIY 200
              T+             D+       +K       FLGFVV  Y ++ + +G  +VL Y
Sbjct: 158 PTRTVMSSEELQIEAAAPDVPSSTIKDLKRSKRLRWFLGFVVFSYFVVIHVVGFLLVLWY 217

Query: 201 FKFVSSARQVLEQKGLKLSTFSIFLIVSTFASCGFVPTNENMIVFKKNLGLLIILIPQAL 260
              VSSA+  L++KG+ ++ FS  + VS+FA+ G VPTNENM +F KN GLL++ I Q L
Sbjct: 218 ISRVSSAKAPLKKKGINIALFSFSVTVSSFANGGLVPTNENMAIFSKNPGLLLLFIGQIL 277

Query: 261 IGNTLFPSCLRICIWSLGKFMKKKKEIVGFLLKNSEEIGYLHLLPKLHSLLLVGSVLGFV 320
            GNTL+P  LRI IW LGK  K K   +  ++KNS+E+ Y +LLPKL +  L  +V+G +
Sbjct: 278 AGNTLYPLFLRILIWFLGKVTKLKD--LKLMIKNSDELQYDYLLPKLPTAFLASTVIGLM 337

Query: 321 LMQFVAIGAMEWRSNALSGLNCVEKLIGILFLSVNSRHSGESIVDLSRLSSAILLLFVAM 380
                  G+++W S+   GL+  +K+I  LF++VN+RHSGE+ +D S ++ A+L+LF+ +
Sbjct: 338 ASLVTLFGSVDWNSSVFDGLSSYQKIINALFMAVNARHSGENSIDCSLIAPAVLVLFIIL 397

Query: 381 MYLPPYTSFMPIKIDEGEEEENQLLEANKRSRKIKRAKFMDNFVFSQLFYLIIFIFIICI 440
           MYLPP T+F    +  G+E+      ANK++++ K    + N  FSQL    +F+ +  I
Sbjct: 398 MYLPPSTTF---ALSNGDEK-----TANKKAKR-KLGLVVRNLAFSQLACNAVFVIVALI 457

Query: 441 TERHNIKEDPLNFNIFNIIFEVISAYGNVGFTMGYNCKR--QIHPQSNCADKSFGFSGKW 500
           TER  ++ DPLNF+  N+IFEVISAYGNVG T GY+C R  ++HP S C DK +  SG W
Sbjct: 458 TERSRLRNDPLNFSALNMIFEVISAYGNVGLTTGYSCSRLQKLHPGSICQDKPYSLSGWW 517

Query: 501 SDNGKVVLIVVMIFGRLKKFNMNKQRPWKL 506
           SD GK++L+ VM++GRLK F       W+L
Sbjct: 518 SDEGKLLLVSVMLYGRLKAFTKGTGEYWRL 529

BLAST of Bhi02G000142 vs. Swiss-Prot
Match: sp|A2YGP9|HKT1_ORYSI (Cation transporter HKT1 OS=Oryza sativa subsp. indica OX=39946 GN=HKT1 PE=1 SV=2)

HSP 1 Score: 365.5 bits (937), Expect = 9.3e-100
Identity = 216/510 (42.35%), Postives = 318/510 (62.35%), Query Frame = 0

Query: 21  MNPFWIQLLYFLFISSFGFFILVVLNPKTTSNNSFNPKKLDLFFTSVSASTVSSMSTVEM 80
           ++PFWIQL YFL IS  G  +L+ L P   SN  F P  +D+ F S SA T+SS+ T+EM
Sbjct: 38  IHPFWIQLSYFLLISILGSVLLMFLKP---SNPEFRPGYIDMLFLSTSALTLSSLITIEM 97

Query: 81  EVFSNSQLIVLTILMFIGGEVFTSMVELQLR---KFKLKFSEQKIACVSSDLSVELGIVR 140
           EV S+SQ++V+T+LM +GGEVF S + L LR   K   +FS  K+    S + +EL  + 
Sbjct: 98  EVLSSSQIVVITLLMLLGGEVFVSFLGLMLRLNHKHNPEFSGDKV----SSVPIELDTIN 157

Query: 141 NNNTL----------DIIILKYNSIK----------FLGFVVLGYLLIFNFLGIGMVLIY 200
           + +T+           I  +  ++IK          FLGFVV  Y ++ +  G  +VL Y
Sbjct: 158 SASTVISCEELQLEAAIPEVPSSTIKDLKRSKRLRWFLGFVVFSYFVVIHVAGFLLVLWY 217

Query: 201 FKFVSSARQVLEQKGLKLSTFSIFLIVSTFASCGFVPTNENMIVFKKNLGLLIILIPQAL 260
              VSSA+  L++KG+ ++ FS  + VS+FA+ G VPTNENM +F KN GLL++ I Q L
Sbjct: 218 ISRVSSAKAPLKKKGINIALFSFSVTVSSFANVGLVPTNENMAIFSKNPGLLLLFIGQIL 277

Query: 261 IGNTLFPSCLRICIWSLGKFMKKKKEIVGFLLKNSEEIGYLHLLPKLHSLLLVGSVLGFV 320
            GNTL+P  LR+ IW LGK  K ++  +  ++KN EE+ Y +LLPKL +  L  +V+G +
Sbjct: 278 AGNTLYPLFLRLLIWFLGKVTKLRE--LKLMIKNPEELQYDYLLPKLPTAFLASTVIGLM 337

Query: 321 LMQFVAIGAMEWRSNALSGLNCVEKLIGILFLSVNSRHSGESIVDLSRLSSAILLLFVAM 380
                  GA++W S+   GL+  +K+I  LF++VN+RHSGE+ +D S ++ A+L+LF+ +
Sbjct: 338 ASLVTLFGAVDWNSSVFDGLSSYQKIINALFMAVNARHSGENSIDCSLIAPAVLVLFIIL 397

Query: 381 MYLPPYTSFMPIKIDEGEEEENQLLEANKRSRKIKRAKFMDNFVFSQLFYLIIFIFIICI 440
           MYLPP T+F    +  G+E+      ANK++++ K    + N  FSQL  + +F+ +  I
Sbjct: 398 MYLPPSTTF---ALSNGDEK-----TANKKAKR-KLGLVVQNLAFSQLACISVFVIVAFI 457

Query: 441 TERHNIKEDPLNFNIFNIIFEVISAYGNVGFTMGYNCKR--QIHPQSNCADKSFGFSGKW 500
           TER  ++ DPLNF+  N+IFE+ISAYGNVG + GY+C R  ++HP S C DK +  SG W
Sbjct: 458 TERSRLRNDPLNFSALNMIFEIISAYGNVGLSTGYSCSRLQKLHPGSICQDKPYSLSGWW 517

Query: 501 SDNGKVVLIVVMIFGRLKKFNMNKQRPWKL 506
           SD GK++L+ VM++GRLK F       W+L
Sbjct: 518 SDEGKLLLVFVMLYGRLKAFTKGTGEYWRL 529

BLAST of Bhi02G000142 vs. TAIR10
Match: AT4G10310.1 (high-affinity K+ transporter 1)

HSP 1 Score: 397.9 bits (1021), Expect = 9.3e-111
Identity = 235/471 (49.89%), Postives = 311/471 (66.03%), Query Frame = 0

Query: 44  VLNPKTTSNNSFNPKKLDLFFTSVSASTVSSMSTVEMEVFSNSQLIVLTILMFIGGEVFT 103
           +  P+TTS     P   DLFFTSVSA TVSSMSTV+MEVFSN+QLI LTILMF+GGE+FT
Sbjct: 42  ITKPRTTS----RPHDFDLFFTSVSAITVSSMSTVDMEVFSNTQLIFLTILMFLGGEIFT 101

Query: 104 SMVELQLRKF-KLKFSEQKIACV----SSDLSVELGIVRNNNT---LDIIILKYNSIKFL 163
           S + L +  F K  F   KI  +    +SD S+E        T     +I +   + K L
Sbjct: 102 SFLNLYVSYFTKFVFPHNKIRHILGSYNSDSSIEDRCDVETVTDYREGLIKIDERASKCL 161

Query: 164 GFVVLGYLLIFNFLGIGMVLIYFKFVSSARQVLEQKGLKLSTFSIFLIVSTFASCGFVPT 223
             VVL Y L+ N +G  ++L+Y  FV +AR VL  K +   TFS+F  VSTFA+CGFVPT
Sbjct: 162 YSVVLSYHLVTNLVGSVLLLVYVNFVKTARDVLSSKEISPLTFSVFTTVSTFANCGFVPT 221

Query: 224 NENMIVFKKNLGLLIILIPQALIGNTLFPSCLRICIWSLGKFMKKKKEIVGFLLKNSEEI 283
           NENMI+F+KN GL+ +LIPQ L+GNTLFP  L + IW L K  K+ +   G++LKN  ++
Sbjct: 222 NENMIIFRKNSGLIWLLIPQVLMGNTLFPCFLVLLIWGLYKITKRDE--YGYILKNHNKM 281

Query: 284 GYLHLLPKLHSLLLVGSVLGFVLMQFVAIGAMEWRSNALSGLNCVEKLIGILFLSVNSRH 343
           GY HLL     +LL  +VLGF+++Q +   A EW S +L G++  EKL+G LF  VNSRH
Sbjct: 282 GYSHLLSVRLCVLLGVTVLGFLIIQLLFFCAFEWTSESLEGMSSYEKLVGSLFQVVNSRH 341

Query: 344 SGESIVDLSRLSSAILLLFVAMMYLPPYTSFMPIKIDEGEEEENQLLEANKRSRKIKRAK 403
           +GE+IVDLS LS AIL+LF+ MMYLPPYT FMP+   +  E+E    + ++  +K+K++ 
Sbjct: 342 TGETIVDLSTLSPAILVLFILMMYLPPYTLFMPLTEQKTIEKEGG-DDDSENGKKVKKS- 401

Query: 404 FMDNFVFSQLFYLIIFIFIICITERHNIKEDPLNFNIFNIIFEVISAYGNVGFTMGYNCK 463
                + SQL +L I IF+I ITER N++ DP+NFN+ NI  EVISAYGNVGFT GY+C+
Sbjct: 402 ---GLIVSQLSFLTICIFLISITERQNLQRDPINFNVLNITLEVISAYGNVGFTTGYSCE 461

Query: 464 RQIH-PQSNCADKSFGFSGKWSDNGKVVLIVVMIFGRLKKFNMNKQRPWKL 506
           R++      C D S+GF+G+WS  GK VLI+VM +GR K+F     R W L
Sbjct: 462 RRVDISDGGCKDASYGFAGRWSPMGKFVLIIVMFYGRFKQFTAKSGRAWIL 501

BLAST of Bhi02G000142 vs. TrEMBL
Match: tr|A0A1S3BWY9|A0A1S3BWY9_CUCME (cation transporter HKT8-like OS=Cucumis melo OX=3656 GN=LOC103494343 PE=4 SV=1)

HSP 1 Score: 774.2 bits (1998), Expect = 1.8e-220
Identity = 416/509 (81.73%), Postives = 455/509 (89.39%), Query Frame = 0

Query: 8   MLRIQRLSCFLIWMNPFWIQLLYFLFISSFGFFILVVLNPKTTSNNSFNPKKLDLFFTSV 67
           M RIQRLSCFLI +NPFWI +LYFLFISSFGF++L++LNPK T      PKKLDLFFTSV
Sbjct: 1   MSRIQRLSCFLILLNPFWIHVLYFLFISSFGFWVLLILNPKLT------PKKLDLFFTSV 60

Query: 68  SASTVSSMSTVEMEVFSNSQLIVLTILMFIGGEVFTSMVELQLRKFKLKFSEQKIACVSS 127
           SASTVSSMST+EMEVFSNSQLIVLTILMFIGGEVFTSMVELQLRK KLKFSE+KIACV +
Sbjct: 61  SASTVSSMSTMEMEVFSNSQLIVLTILMFIGGEVFTSMVELQLRKLKLKFSEKKIACVEN 120

Query: 128 DL--SVELGIVRNNNTLDIIILKYNSIKFLGFVVLGYLLIFNFLGIGMVLIYFKFVSS-A 187
           DL  S+ELG + ++N  D  ILKYNSIKFLG+VVLGYLLIFNFLGI MVL+Y K VSS A
Sbjct: 121 DLNSSLELGDIDDHNNFD--ILKYNSIKFLGYVVLGYLLIFNFLGIVMVLVYLKCVSSTA 180

Query: 188 RQVLEQKGLKLSTFSIFLIVSTFASCGFVPTNENMIVFKKNLGLLIILIPQALIGNTLFP 247
            +VL+ KGLK STFS+F+IVSTFASCGFVPTNENMIVF+KNLGLLIILIPQALIGNTLFP
Sbjct: 181 TEVLKSKGLKTSTFSVFVIVSTFASCGFVPTNENMIVFRKNLGLLIILIPQALIGNTLFP 240

Query: 248 SCLRICIWSLGKFMKKKKEIVGFLLKNSEEIGYLHLLPKLHSLLLVGSVLGFVLMQFVAI 307
           SCLR CIWSLGK MKKKKE+VGFLL+NSEEIGYLHLLPKL+SL+LV SV GFVL+Q VAI
Sbjct: 241 SCLRFCIWSLGKIMKKKKEVVGFLLENSEEIGYLHLLPKLYSLVLVASVFGFVLIQSVAI 300

Query: 308 GAMEWRS---NALSGLNCVEKLIGILFLSVNSRHSGESIVDLSRLSSAILLLFVAMMYLP 367
           GAMEWRS   N  SGLN VEKLIGILFLSVNSRHSGESIVDLS LSSAIL+LFVAMMYLP
Sbjct: 301 GAMEWRSSSNNGFSGLNWVEKLIGILFLSVNSRHSGESIVDLSTLSSAILVLFVAMMYLP 360

Query: 368 PYTSFMPIKIDEGEE----EENQLLEANKRSRKIKRAKFMDNFVFSQLFYLIIFIFIICI 427
           PYTSFMPIKI+EGEE       +++E NK+ RK KRAKF+DNFVFSQL YLIIFIFIICI
Sbjct: 361 PYTSFMPIKIEEGEEGXXXXXXEIMEGNKKRRKTKRAKFVDNFVFSQLSYLIIFIFIICI 420

Query: 428 TERHNIKEDPLNFNIFNIIFEVISAYGNVGFTMGYNCKRQIHPQSNCADKSFGFSGKWSD 487
           TERHNIK+DPLNFNIFNI+FEVISAYGNVGF+MGY+C RQ+HPQSNC D SFGFSGKWSD
Sbjct: 421 TERHNIKQDPLNFNIFNIVFEVISAYGNVGFSMGYSCGRQVHPQSNCVDMSFGFSGKWSD 480

Query: 488 NGKVVLIVVMIFGRLKKFNMNKQRPWKLL 507
           NGK+VLIVVMIFGRLKKFNMN+QR WKLL
Sbjct: 481 NGKLVLIVVMIFGRLKKFNMNRQRAWKLL 501

BLAST of Bhi02G000142 vs. TrEMBL
Match: tr|M5XNP4|M5XNP4_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_1G066800 PE=4 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 4.7e-157
Identity = 308/517 (59.57%), Postives = 383/517 (74.08%), Query Frame = 0

Query: 23  PFWIQLLYFLFISSFGFFILVVLNPKTTSNNSFNPKKLDLFFTSVSASTVSSMSTVEMEV 82
           PFWI+L+YF+ +S  GF++L  L P+T  + SF P+ LDLFFTSVSASTVSSMSTVEMEV
Sbjct: 46  PFWIELVYFVTLSFLGFWVLKALKPRT--DPSFRPRNLDLFFTSVSASTVSSMSTVEMEV 105

Query: 83  FSNSQLIVLTILMFIGGEVFTSMVELQLRKFKLK-FSEQKIACVSSDLS----------- 142
           FSN+QLI+LTILMFIGGEVFTSMV LQL K KL   +++K+A  SSD+S           
Sbjct: 106 FSNAQLIILTILMFIGGEVFTSMVGLQLWKLKLMLLTKEKVASASSDISPSSTPTNNIVF 165

Query: 143 --VELGIVRN-------------------NNTLDIIILKYNSIKFLGFVVLGYLLIFNFL 202
             VELG + +                   +  LD   LK+NSIKFLG VV+GYLL+ + L
Sbjct: 166 DHVELGSLASKPEFQDLNLGVEVYDQDQIDKPLDGESLKFNSIKFLGCVVVGYLLVIHGL 225

Query: 203 GIGMVLIYFKFVSSARQVLEQKGLKLSTFSIFLIVSTFASCGFVPTNENMIVFKKNLGLL 262
           G+ MV +Y   +SSAR +L+ K L + TFS F  VSTF+SCGFVPTNENM+VF KN GLL
Sbjct: 226 GVLMVSLYLAVISSARNILKNKELNMHTFSFFTTVSTFSSCGFVPTNENMMVFSKNTGLL 285

Query: 263 IILIPQALIGNTLFPSCLRICIWSLGKFMKKKKEIVGFLLKNSEEIGYLHLLPKLHSLLL 322
           +ILIPQ L+GNTL PSCLR  IW LGK  KKK+ +  +LLKN+ ++G+LHLLP LHS LL
Sbjct: 286 LILIPQVLLGNTLLPSCLRFSIWVLGKIFKKKEHLCNYLLKNTRKLGFLHLLPSLHSSLL 345

Query: 323 VGSVLGFVLMQFVAIGAMEWRSNALSGLNCVEKLIGILFLSVNSRHSGESIVDLSRLSSA 382
           V +VLGF+++Q VA  ++EW S++LSGLN  +K+IG LF SVNSRH+GE+IVDLS +S A
Sbjct: 346 VVTVLGFIMVQLVAFLSLEWSSDSLSGLNSYQKIIGALFQSVNSRHTGETIVDLSIISPA 405

Query: 383 ILLLFVAMMYLPPYTSFMPIKIDEGEEEENQLLEANKRSRKIKRAKFMDNFVFSQLFYLI 442
           IL+LFV MMYLPPYTSF+PIK    ++++N L    +  RK +R K ++N +FSQL YL 
Sbjct: 406 ILVLFVVMMYLPPYTSFLPIK----DDDQNPLQPQER--RKTRRGKVVENLIFSQLSYLA 465

Query: 443 IFIFIICITERHNIKEDPLNFNIFNIIFEVISAYGNVGFTMGYNCKRQIHPQSNCADKSF 502
           IFI ++CITER  +KEDPLNFN+ NI+ EVISAYGNVGFT GY+CKRQIHP  +C DK +
Sbjct: 466 IFIVLVCITERKQMKEDPLNFNVLNIVIEVISAYGNVGFTTGYSCKRQIHPNGDCQDKWY 525

Query: 503 GFSGKWSDNGKVVLIVVMIFGRLKKFNMNKQRPWKLL 507
           GFSG+WSD GK+VLI VM FGRLKKFNM+  R WKLL
Sbjct: 526 GFSGRWSDQGKLVLIAVMFFGRLKKFNMDGGRAWKLL 554

BLAST of Bhi02G000142 vs. TrEMBL
Match: tr|A0A2P6QS71|A0A2P6QS71_ROSCH (Putative cation transporter OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr4g0398141 PE=4 SV=1)

HSP 1 Score: 557.8 bits (1436), Expect = 2.6e-155
Identity = 297/515 (57.67%), Postives = 383/515 (74.37%), Query Frame = 0

Query: 18  LIWMNPFWIQLLYFLFISSFGFFILVVLNPKTTSNNSFNPKKLDLFFTSVSASTVSSMST 77
           L+   PFW++L+YF+ +S  G+++L VL  +TT  +SF P+ LDLFFTSVSA+TVSSMST
Sbjct: 41  LLRAKPFWVELVYFVTLSFLGYWVLKVLPVRTT--DSFTPRNLDLFFTSVSATTVSSMST 100

Query: 78  VEMEVFSNSQLIVLTILMFIGGEVFTSMVELQLRKFKLKF---SEQKIACVSSDLS---- 137
           VEMEVFSN QLI++TILMFIGGEVFTSMV L  +KFKL     +++KI  V+SD+S    
Sbjct: 101 VEMEVFSNGQLILMTILMFIGGEVFTSMVGLHFKKFKLMMMMSTKEKITSVNSDVSSSSS 160

Query: 138 --------VELGIVRN----NNTLDII-------ILKYNSIKFLGFVVLGYLLIFNFLGI 197
                   +ELG+       +  +DII       +   NSI  LG+VVL YLL+ +FLG+
Sbjct: 161 PAKAIDENIELGLAIKPEFPHEQIDIIAAVDDQSLTLQNSINLLGYVVLAYLLVVHFLGV 220

Query: 198 GMVLIYFKFVSSARQVLEQKGLKLSTFSIFLIVSTFASCGFVPTNENMIVFKKNLGLLII 257
            MVL+Y  F SSAR +++ KG+ + TFS F  VSTF+SCGFVPTNENMIVF KN GLL+I
Sbjct: 221 AMVLVYLAFTSSARNIVKNKGINVHTFSFFTTVSTFSSCGFVPTNENMIVFSKNSGLLLI 280

Query: 258 LIPQALIGNTLFPSCLRICIWSLGKFMKKKKEIVGFLLKNSEEIGYLHLLPKLHSLLLVG 317
           LIPQ L+GNTL+PSCLR  IW LGK  KK+++   +LLKN++E+GYLHLLP LHS LLV 
Sbjct: 281 LIPQVLLGNTLYPSCLRFSIWLLGKNFKKQRDSCNYLLKNTKEVGYLHLLPSLHSSLLVV 340

Query: 318 SVLGFVLMQFVAIGAMEWRSNALSGLNCVEKLIGILFLSVNSRHSGESIVDLSRLSSAIL 377
           +V GF+L+Q +   +MEW S +LSGLN  +K+IG LF +VNSRH+GE+IVDLS +S AIL
Sbjct: 341 TVFGFILVQLLPFASMEWSSESLSGLNSYQKIIGSLFQTVNSRHTGETIVDLSIISPAIL 400

Query: 378 LLFVAMMYLPPYTSFMPIKIDEGEEEENQLLEANKRSRKIKRAKFMDNFVFSQLFYLIIF 437
           +LF+ MMYLPPYTSF+P       E +   L+++ + +K +R K MDN +FSQL YL IF
Sbjct: 401 VLFIVMMYLPPYTSFLP-----AIENDRYDLQSHDQRKKKRRGKLMDNLIFSQLSYLTIF 460

Query: 438 IFIICITERHNIKEDPLNFNIFNIIFEVISAYGNVGFTMGYNCKRQIHPQSNCADKSFGF 497
           I ++CITER ++KEDPLNFN+ NI+ EVISAYGNVGFT GY+CKRQ++P  +C D+ +GF
Sbjct: 461 IILVCITERKSLKEDPLNFNVLNIVLEVISAYGNVGFTTGYSCKRQLNPNGDCQDRLYGF 520

Query: 498 SGKWSDNGKVVLIVVMIFGRLKKFNMNKQRPWKLL 507
           SG+WSD GK+VLI VM FGRLKKFNM+  + WKLL
Sbjct: 521 SGRWSDEGKIVLIAVMFFGRLKKFNMDGGKGWKLL 548

BLAST of Bhi02G000142 vs. TrEMBL
Match: tr|A0A059CQU6|A0A059CQU6_EUCGR (Uncharacterized protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_C02202 PE=4 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 1.4e-148
Identity = 293/527 (55.60%), Postives = 381/527 (72.30%), Query Frame = 0

Query: 15  SCF----LIWMNPFWIQLLYFLFISSFGFFILVVLNPKTTSNNSFNPKKLDLFFTSVSAS 74
           SCF    L+ +N F IQ+ YF+F+S  GF++L  L P+T   NS+ P+ LDLFFTSVSA+
Sbjct: 34  SCFLRFLLLQVNSFCIQVFYFVFLSFLGFWVLKALRPRT---NSYRPRDLDLFFTSVSAT 93

Query: 75  TVSSMSTVEMEVFSNSQLIVLTILMFIGGEVFTSMVELQLRKFKLKF---SEQKIACVSS 134
           TVSSMSTVEMEVFSNSQL+V+T+LMFIGGEVF S+V L LRK KL++   +E+++ CV+S
Sbjct: 94  TVSSMSTVEMEVFSNSQLVVMTVLMFIGGEVFVSLVGLHLRKSKLRWHIKTEEQVTCVNS 153

Query: 135 DL-----------SVELGIV----------------RNNNTLDIIILKYNSIKFLGFVVL 194
           DL            +E+G+V                +   +LD+  L+Y S++FL FVVL
Sbjct: 154 DLCPSNPTNDKVEHIEIGVVEKLDCLNSQVEPQLYSQQGESLDLDYLQYCSVRFLCFVVL 213

Query: 195 GYLLIFNFLGIGMVLIYFKFVSSARQVLEQKGLKLSTFSIFLIVSTFASCGFVPTNENMI 254
           GYLL  + LG+  V +Y  F+SSAR VL++KGLK+ TFS+F +VSTFASCGFVPTNENMI
Sbjct: 214 GYLLGVHVLGVATVSLYMAFISSARDVLKKKGLKMVTFSVFTVVSTFASCGFVPTNENMI 273

Query: 255 VFKKNLGLLIILIPQALIGNTLFPSCLRICIWSLGKFMKKKKEIVGFLLKNSEEIGYLHL 314
           VF KN GLL++LIPQ L+GNTLFPS LR  +W +G+F KK +  + +LL  + E+GY HL
Sbjct: 274 VFSKNSGLLLLLIPQVLLGNTLFPSTLRFMLWLMGRFTKKAE--IKYLLSRTSEVGYKHL 333

Query: 315 LPKLHSLLLVGSVLGFVLMQFVAIGAMEWRSNALSGLNCVEKLIGILFLSVNSRHSGESI 374
           LP LHS LLV +VLGFV +QF+    M+W S +L+GL+  EK++G++FL VN+RH+GE+I
Sbjct: 334 LPSLHSSLLVVTVLGFVGVQFIMFCLMQWHSESLNGLSSYEKIVGVIFLCVNTRHTGETI 393

Query: 375 VDLSRLSSAILLLFVAMMYLPPYTSFMPIKIDEGEEEENQLLEANKRSRKIKRA--KFMD 434
           VDLS ++ AIL++FV MMYLPPYTSF+P+K         + L  N   RK KR+    ++
Sbjct: 394 VDLSTVAPAILVVFVVMMYLPPYTSFLPVK-------GGERLPGNGDRRKPKRSYKLLLE 453

Query: 435 NFVFSQLFYLIIFIFIICITERHNIKEDPLNFNIFNIIFEVISAYGNVGFTMGYNCKRQI 494
           N   SQL YL IFI +IC+TER  +KEDPLNFN+ NI+ EVISAYGNVGFTMGY+C+RQ+
Sbjct: 454 NLKLSQLSYLAIFIVMICVTERKKMKEDPLNFNVLNIVVEVISAYGNVGFTMGYSCQRQL 513

Query: 495 HPQSNCADKSFGFSGKWSDNGKVVLIVVMIFGRLKKFNMNKQRPWKL 506
            P   C DK +GFSGKWSD GK++LI+VMIFGRLKKFNM   + W L
Sbjct: 514 KPVEGCEDKWYGFSGKWSDQGKIILIIVMIFGRLKKFNMKGGQAWVL 548

BLAST of Bhi02G000142 vs. TrEMBL
Match: tr|A0A218XPL5|A0A218XPL5_PUNGR (Uncharacterized protein OS=Punica granatum OX=22663 GN=CDL15_Pgr015963 PE=4 SV=1)

HSP 1 Score: 533.9 bits (1374), Expect = 4.0e-148
Identity = 290/499 (58.12%), Postives = 366/499 (73.35%), Query Frame = 0

Query: 22  NPFWIQLLYFLFISSFGFFILVVLNPKTTSNNSFNPKKLDLFFTSVSASTVSSMSTVEME 81
           N F +Q+LYF+ +SS G+++L  LNP+T    SF+P+ LDLFFTSVSA+TVSSMSTVEME
Sbjct: 35  NSFLLQVLYFVCLSSLGYWVLKCLNPRTP--GSFDPRSLDLFFTSVSAATVSSMSTVEME 94

Query: 82  VFSNSQLIVLTILMFIGGEVFTSMVELQLRKFKLKF---SEQKIACVSSDLS-------- 141
           VFSN+QLIVLT+LMF+GGEVF SMV L LRK +++    ++ K+A V SDLS        
Sbjct: 95  VFSNTQLIVLTLLMFVGGEVFASMVGLHLRKHRMRMVLKTDDKVASVKSDLSIHSMGSMD 154

Query: 142 ----VELGIVRNNNTL---DIIILKYNSIKFLGFVVLGYLLIFNFLGIGMVLIYFKFVSS 201
               +ELG+     +    D   L+Y S++FLG VVL YL++   LG+ +V IY + VS+
Sbjct: 155 IIDGIELGLTEKTQSFGPDDCEYLRYRSVRFLGVVVLCYLVVVQALGVAVVSIYLRVVST 214

Query: 202 ARQVLEQKGLKLSTFSIFLIVSTFASCGFVPTNENMIVFKKNLGLLIILIPQALIGNTLF 261
           AR VL +KGL   TFS+F IVSTFASCGFVPTNENMIVF KN  LL+ILIPQ L+GNTLF
Sbjct: 215 ARDVLRKKGLNTLTFSVFTIVSTFASCGFVPTNENMIVFSKNSALLLILIPQVLLGNTLF 274

Query: 262 PSCLRICIWSLGKFMKKKKEIVGFLLKNSEEIGYLHLLPKLHSLLLVGSVLGFVLMQFVA 321
           PSCLR C+W + KF  K +    +LL+NS EI YLHLL  LHS LLV +V G + +QFV 
Sbjct: 275 PSCLRFCVWLVHKFANKAE--TSYLLRNSSEIRYLHLLSGLHSRLLVATVFGLIGIQFVV 334

Query: 322 IGAMEWRSNALSGLNCVEKLIGILFLSVNSRHSGESIVDLSRLSSAILLLFVAMMYLPPY 381
             AM+W +  L+GL   EK++G+LF  VNSRH+GE+IVDLS ++ A+L+LFV MMYLPPY
Sbjct: 335 FSAMQWNTEVLAGLGQHEKVVGVLFQCVNSRHTGETIVDLSTIAPAVLVLFVIMMYLPPY 394

Query: 382 TSFMPIK-IDEGEEEENQLLEANKRSRKIKRAKFMDNFVFSQLFYLIIFIFIICITERHN 441
            SF+P+K IDEG         A +R+R   R K ++N +FSQL YL IFI +ICITER  
Sbjct: 395 ASFLPVKDIDEGRP------RAVERNRPEIRRKIIENLIFSQLSYLAIFIIMICITEREK 454

Query: 442 IKEDPLNFNIFNIIFEVISAYGNVGFTMGYNCKRQIHPQSNCADKSFGFSGKWSDNGKVV 501
           +KEDPLNFN+  I+ EVISAYGNVGFT GY+CKRQ+ P++ C DK FGFSG+WSD GK++
Sbjct: 455 MKEDPLNFNVLTIVVEVISAYGNVGFTTGYSCKRQLKPEAGCRDKWFGFSGRWSDQGKII 514

BLAST of Bhi02G000142 vs. NCBI nr
Match: XP_008453695.1 (PREDICTED: cation transporter HKT8-like [Cucumis melo])

HSP 1 Score: 774.2 bits (1998), Expect = 2.6e-220
Identity = 416/509 (81.73%), Postives = 455/509 (89.39%), Query Frame = 0

Query: 8   MLRIQRLSCFLIWMNPFWIQLLYFLFISSFGFFILVVLNPKTTSNNSFNPKKLDLFFTSV 67
           M RIQRLSCFLI +NPFWI +LYFLFISSFGF++L++LNPK T      PKKLDLFFTSV
Sbjct: 1   MSRIQRLSCFLILLNPFWIHVLYFLFISSFGFWVLLILNPKLT------PKKLDLFFTSV 60

Query: 68  SASTVSSMSTVEMEVFSNSQLIVLTILMFIGGEVFTSMVELQLRKFKLKFSEQKIACVSS 127
           SASTVSSMST+EMEVFSNSQLIVLTILMFIGGEVFTSMVELQLRK KLKFSE+KIACV +
Sbjct: 61  SASTVSSMSTMEMEVFSNSQLIVLTILMFIGGEVFTSMVELQLRKLKLKFSEKKIACVEN 120

Query: 128 DL--SVELGIVRNNNTLDIIILKYNSIKFLGFVVLGYLLIFNFLGIGMVLIYFKFVSS-A 187
           DL  S+ELG + ++N  D  ILKYNSIKFLG+VVLGYLLIFNFLGI MVL+Y K VSS A
Sbjct: 121 DLNSSLELGDIDDHNNFD--ILKYNSIKFLGYVVLGYLLIFNFLGIVMVLVYLKCVSSTA 180

Query: 188 RQVLEQKGLKLSTFSIFLIVSTFASCGFVPTNENMIVFKKNLGLLIILIPQALIGNTLFP 247
            +VL+ KGLK STFS+F+IVSTFASCGFVPTNENMIVF+KNLGLLIILIPQALIGNTLFP
Sbjct: 181 TEVLKSKGLKTSTFSVFVIVSTFASCGFVPTNENMIVFRKNLGLLIILIPQALIGNTLFP 240

Query: 248 SCLRICIWSLGKFMKKKKEIVGFLLKNSEEIGYLHLLPKLHSLLLVGSVLGFVLMQFVAI 307
           SCLR CIWSLGK MKKKKE+VGFLL+NSEEIGYLHLLPKL+SL+LV SV GFVL+Q VAI
Sbjct: 241 SCLRFCIWSLGKIMKKKKEVVGFLLENSEEIGYLHLLPKLYSLVLVASVFGFVLIQSVAI 300

Query: 308 GAMEWRS---NALSGLNCVEKLIGILFLSVNSRHSGESIVDLSRLSSAILLLFVAMMYLP 367
           GAMEWRS   N  SGLN VEKLIGILFLSVNSRHSGESIVDLS LSSAIL+LFVAMMYLP
Sbjct: 301 GAMEWRSSSNNGFSGLNWVEKLIGILFLSVNSRHSGESIVDLSTLSSAILVLFVAMMYLP 360

Query: 368 PYTSFMPIKIDEGEE----EENQLLEANKRSRKIKRAKFMDNFVFSQLFYLIIFIFIICI 427
           PYTSFMPIKI+EGEE       +++E NK+ RK KRAKF+DNFVFSQL YLIIFIFIICI
Sbjct: 361 PYTSFMPIKIEEGEEGXXXXXXEIMEGNKKRRKTKRAKFVDNFVFSQLSYLIIFIFIICI 420

Query: 428 TERHNIKEDPLNFNIFNIIFEVISAYGNVGFTMGYNCKRQIHPQSNCADKSFGFSGKWSD 487
           TERHNIK+DPLNFNIFNI+FEVISAYGNVGF+MGY+C RQ+HPQSNC D SFGFSGKWSD
Sbjct: 421 TERHNIKQDPLNFNIFNIVFEVISAYGNVGFSMGYSCGRQVHPQSNCVDMSFGFSGKWSD 480

Query: 488 NGKVVLIVVMIFGRLKKFNMNKQRPWKLL 507
           NGK+VLIVVMIFGRLKKFNMN+QR WKLL
Sbjct: 481 NGKLVLIVVMIFGRLKKFNMNRQRAWKLL 501

BLAST of Bhi02G000142 vs. NCBI nr
Match: XP_023516746.1 (cation transporter HKT8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 695.7 bits (1794), Expect = 1.2e-196
Identity = 370/499 (74.15%), Postives = 417/499 (83.57%), Query Frame = 0

Query: 8   MLRIQRLSCFLIWMNPFWIQLLYFLFISSFGFFILVVLNPKTTSNNSFNPKKLDLFFTSV 67
           M R+QRLSCFLI ++PFWI L YFLFISS GF  L+VL PKT S + F+P+ LDLFFTSV
Sbjct: 1   MSRMQRLSCFLIRVHPFWIHLFYFLFISSLGFLALLVLKPKTGSYHPFHPQNLDLFFTSV 60

Query: 68  SASTVSSMSTVEMEVFSNSQLIVLTILMFIGGEVFTSMVELQLRKFKLKFSEQKIACVSS 127
           SA+TVSSMS VEME FS+ QLIVL++LMFIGGEVFTSMV LQ RKF LKF + KIA V  
Sbjct: 61  SAATVSSMSAVEMEAFSDPQLIVLSVLMFIGGEVFTSMVALQFRKFNLKFPDLKIASVPI 120

Query: 128 DLSVELGIVRNNNTLDIIILKYNSIKFLGFVVLGYLLIFNFLGIGMVLIYFKFVSSARQV 187
           DL  +              LKY SIKFLGFVV GYL++ NFLGI MVLIY K   +AR V
Sbjct: 121 DLEAKFDPPPPER------LKYKSIKFLGFVVFGYLILSNFLGIVMVLIYLKLAPTARDV 180

Query: 188 LEQKGLKLSTFSIFLIVSTFASCGFVPTNENMIVFKKNLGLLIILIPQALIGNTLFPSCL 247
           L+QKGLK +TFS+F++VSTFASCGFVPTNENMIVF+KNLGLL++LIPQAL+GNTLFPSCL
Sbjct: 181 LKQKGLKTTTFSVFVVVSTFASCGFVPTNENMIVFRKNLGLLLVLIPQALLGNTLFPSCL 240

Query: 248 RICIWSLGKFMKKKK-EIVGFLLKNSEEIGYLHLLPKLHSLLLVGSVLGFVLMQFVAIGA 307
           R  IW LGK +KKKK +IVGFLL+NSEEIG+LHLLPKL S LLVGSV GFV++Q VAIGA
Sbjct: 241 RASIWGLGKVVKKKKLKIVGFLLENSEEIGFLHLLPKLDSWLLVGSVCGFVVVQVVAIGA 300

Query: 308 MEWRSNALSGLNCVEKLIGILFLSVNSRHSGESIVDLSRLSSAILLLFVAMMYLPPYTSF 367
           MEW S ALSGLNC EKL+GILFLSVNSRHSGE+I+DLS LS AIL+LF AMMYLPPYTSF
Sbjct: 301 MEWSSGALSGLNCGEKLVGILFLSVNSRHSGEAILDLSILSPAILVLFAAMMYLPPYTSF 360

Query: 368 MPIKIDEGEEEENQLLEANKRSRKIKRAKFMDNFVFSQLFYLIIFIFIICITERHNIKED 427
           MPIKIDEGE             R+IKRAKFMDNF+FSQLFYLIIF+FIIC+TERHNIK D
Sbjct: 361 MPIKIDEGE-------------RRIKRAKFMDNFLFSQLFYLIIFVFIICVTERHNIKHD 420

Query: 428 PLNFNIFNIIFEVISAYGNVGFTMGYNCKRQIHPQSNCADKSFGFSGKWSDNGKVVLIVV 487
           PLNF++FN+IFEVISAYGNVGF+MGY+CKRQ++P S C DKSFGFSGKWSDNGK+VLIVV
Sbjct: 421 PLNFSLFNVIFEVISAYGNVGFSMGYSCKRQLYPSSVCVDKSFGFSGKWSDNGKLVLIVV 480

Query: 488 MIFGRLKKFNMNKQRPWKL 506
           MIFGRLKKFNMNKQR WKL
Sbjct: 481 MIFGRLKKFNMNKQRAWKL 480

BLAST of Bhi02G000142 vs. NCBI nr
Match: XP_022921389.1 (probable cation transporter HKT6 [Cucurbita moschata])

HSP 1 Score: 688.7 bits (1776), Expect = 1.5e-194
Identity = 366/499 (73.35%), Postives = 416/499 (83.37%), Query Frame = 0

Query: 8   MLRIQRLSCFLIWMNPFWIQLLYFLFISSFGFFILVVLNPKTTSNNSFNPKKLDLFFTSV 67
           M R+QRLSCFLI ++PFWI L YFL IS FGF  L+VL PKT S + F+P+ LDLFFTSV
Sbjct: 1   MSRMQRLSCFLIRVHPFWIHLFYFLSISCFGFLALLVLKPKTASYDHFHPQNLDLFFTSV 60

Query: 68  SASTVSSMSTVEMEVFSNSQLIVLTILMFIGGEVFTSMVELQLRKFKLKFSEQKIACVSS 127
           SA+TVSSMS VEME FS+ QLIVL++LMFIGGEVFTSMV LQ RKF LKF + KIA V S
Sbjct: 61  SAATVSSMSAVEMEAFSDPQLIVLSVLMFIGGEVFTSMVALQFRKFNLKFPDHKIASVPS 120

Query: 128 DLSVELGIVRNNNTLDIIILKYNSIKFLGFVVLGYLLIFNFLGIGMVLIYFKFVSSARQV 187
           DL  +              LKY SIKFLGFVV GYL++FNFLGI MVLIY K   +AR V
Sbjct: 121 DLEAKFDPPPPER------LKYKSIKFLGFVVFGYLILFNFLGIVMVLIYLKLAPTARDV 180

Query: 188 LEQKGLKLSTFSIFLIVSTFASCGFVPTNENMIVFKKNLGLLIILIPQALIGNTLFPSCL 247
           L+QKGLK +TFS+F++VSTFASCGFVPTNENMIVF+KNLGLL++LIPQAL+GNTLFPSCL
Sbjct: 181 LKQKGLKTTTFSVFVVVSTFASCGFVPTNENMIVFRKNLGLLLVLIPQALLGNTLFPSCL 240

Query: 248 RICIWSLGKFMKKKK-EIVGFLLKNSEEIGYLHLLPKLHSLLLVGSVLGFVLMQFVAIGA 307
           R  IW LGK +KKKK +IVGFLL+NSEEIG+LHLLPKL S LLVGSV GFV++Q VAIGA
Sbjct: 241 RASIWGLGKVVKKKKLKIVGFLLENSEEIGFLHLLPKLDSWLLVGSVCGFVVVQVVAIGA 300

Query: 308 MEWRSNALSGLNCVEKLIGILFLSVNSRHSGESIVDLSRLSSAILLLFVAMMYLPPYTSF 367
           MEW S ALSGLNC EKL+GILFLSVNSRHSGE+IVDLS LS AIL+LF AMMYLPPYTSF
Sbjct: 301 MEWSSGALSGLNCGEKLVGILFLSVNSRHSGEAIVDLSILSPAILVLFAAMMYLPPYTSF 360

Query: 368 MPIKIDEGEEEENQLLEANKRSRKIKRAKFMDNFVFSQLFYLIIFIFIICITERHNIKED 427
           MPIKIDEG+              +IKRAKFMDNF+FSQLFYLI+F+FIIC+TER NIK+D
Sbjct: 361 MPIKIDEGD-------------GRIKRAKFMDNFLFSQLFYLIMFVFIICVTERRNIKDD 420

Query: 428 PLNFNIFNIIFEVISAYGNVGFTMGYNCKRQIHPQSNCADKSFGFSGKWSDNGKVVLIVV 487
            LNF++FNIIFEVISAYGNVGF++GY+CKRQ++P S C DKSFGF+GKWSDNGK+VLIVV
Sbjct: 421 SLNFSLFNIIFEVISAYGNVGFSLGYSCKRQLYPSSVCVDKSFGFAGKWSDNGKLVLIVV 480

Query: 488 MIFGRLKKFNMNKQRPWKL 506
           MIFGRLKKFNMNKQR WKL
Sbjct: 481 MIFGRLKKFNMNKQRAWKL 480

BLAST of Bhi02G000142 vs. NCBI nr
Match: XP_022134789.1 (sodium transporter HKT1-like [Momordica charantia])

HSP 1 Score: 683.3 bits (1762), Expect = 6.1e-193
Identity = 373/502 (74.30%), Postives = 415/502 (82.67%), Query Frame = 0

Query: 17  FLIWMNPFWIQLLYFLFISSFGFFILVVLNPKTTSNNSFNPKKLDLFFTSVSASTVSSMS 76
           FL+ MNPFWIQL YFLFIS+FG ++L VL PKT   +S+ P  LDL FTSVSASTVSSMS
Sbjct: 42  FLLLMNPFWIQLFYFLFISAFGLWVLAVLKPKT--YDSYRPANLDLLFTSVSASTVSSMS 101

Query: 77  TVEMEVFSNSQLIVLTILMFIGGEVFTSMVELQLRKFKLKFSEQKIACVSSDLS------ 136
           +VEMEVFS+SQLIVL +LMF+GGEVFTSMV +QLRK KLK SE KI  V S LS      
Sbjct: 102 SVEMEVFSDSQLIVLIVLMFVGGEVFTSMVGIQLRKLKLKLSEDKITSVPSVLSTISSNP 161

Query: 137 ------VELGIVRNNNTLDIIILKYNSIKFLGFVVLGYLLIFNFLGIGMVLIYFKFVSSA 196
                 +ELG+VR  +      LK+NSIK LG VVL YLL  + LGIGMVL+Y + VSSA
Sbjct: 162 QNILSQIELGVVRKLDHQPERQLKHNSIKLLGIVVLSYLLTIHLLGIGMVLVYMETVSSA 221

Query: 197 RQVLEQKGLKLSTFSIFLIVSTFASCGFVPTNENMIVFKKNLGLLIILIPQALIGNTLFP 256
           R+VL +KGLK STF+IFL VSTFASCGFVPTNENMIVF KNLGLL +LIPQAL+GNTLFP
Sbjct: 222 REVLSKKGLKTSTFAIFLTVSTFASCGFVPTNENMIVFSKNLGLLAMLIPQALLGNTLFP 281

Query: 257 SCLRICIWSLGKFMKKKKEIVGFLLKNSEEIGYLHLLPKLHSLLLVGSVLGFVLMQFVAI 316
            CLR  IWSLGKF KK +    FLLKNSEEIGYLHLLP+LHSLLL  +V GFVL+Q VAI
Sbjct: 282 CCLRFSIWSLGKFTKKSE--TRFLLKNSEEIGYLHLLPRLHSLLLASTVFGFVLIQSVAI 341

Query: 317 GAMEWRSNALSGLNCVEKLIGILFLSVNSRHSGESIVDLSRLSSAILLLFVAMMYLPPYT 376
             MEW S ALSGLN +EKL+G+LFLSVNSRHSGESIVDLS LS AIL LFVAMMYLPPYT
Sbjct: 342 WFMEWSSEALSGLNSIEKLLGVLFLSVNSRHSGESIVDLSMLSPAILFLFVAMMYLPPYT 401

Query: 377 SFMPIKIDEGEEEENQLLEANKRSRKIKRAKFMDNFVFSQLFYLIIFIFIICITERHNIK 436
           SFMPIK D GEE ENQ LEANK  +K KRAKF+DNF+FSQ+ YL+IFIF+ICITERHNIK
Sbjct: 402 SFMPIKGD-GEEGENQ-LEANKIIKKNKRAKFVDNFIFSQISYLLIFIFVICITERHNIK 461

Query: 437 EDPLNFNIFNIIFEVISAYGNVGFTMGYNCKRQIHPQSNCADKSFGFSGKWSDNGKVVLI 496
           EDPLNFN+FNIIFEVISAYGNVGFTMGY+CKRQ+HPQSNC DKSFGFSGKWS  GK+VLI
Sbjct: 462 EDPLNFNLFNIIFEVISAYGNVGFTMGYSCKRQLHPQSNCVDKSFGFSGKWSSKGKIVLI 521

Query: 497 VVMIFGRLKKFNMNKQRPWKLL 507
           VVMIFGRLKKFNMN++R WKLL
Sbjct: 522 VVMIFGRLKKFNMNRERAWKLL 537

BLAST of Bhi02G000142 vs. NCBI nr
Match: XP_004150749.2 (PREDICTED: sodium transporter HKT1-like [Cucumis sativus])

HSP 1 Score: 667.9 bits (1722), Expect = 2.7e-188
Identity = 356/436 (81.65%), Postives = 394/436 (90.37%), Query Frame = 0

Query: 78  VEMEVFSNSQLIVLTILMFIGGEVFTSMVELQLRKFKLKFSEQKIACVSSDL---SVELG 137
           +EMEVFSNSQLIVLT+LMFIGGEVFTSMVELQLRK KLKFSEQKIACV +D    ++ELG
Sbjct: 1   MEMEVFSNSQLIVLTVLMFIGGEVFTSMVELQLRKLKLKFSEQKIACVENDFNNNNLELG 60

Query: 138 IVRNNNTLDIIILKYNSIKFLGFVVLGYLLIFNFLGIGMVLIYFKFVSS-ARQVLEQKGL 197
           ++  NN+ D IILKY+SIKFLG+VVLGYLLIFNFLGI +VLIY K VSS AR+VL+ KGL
Sbjct: 61  VI--NNSFD-IILKYDSIKFLGYVVLGYLLIFNFLGIALVLIYLKRVSSTAREVLKSKGL 120

Query: 198 KLSTFSIFLIVSTFASCGFVPTNENMIVFKKNLGLLIILIPQALIGNTLFPSCLRICIWS 257
           + STFS+F++VSTFASCGFVPTNENMIVF+KNLGLL+ILIPQALIGNTLFPSCLR CIWS
Sbjct: 121 RTSTFSLFVVVSTFASCGFVPTNENMIVFRKNLGLLLILIPQALIGNTLFPSCLRFCIWS 180

Query: 258 LGKFMKKKKEIVGFLLKNSEEIGYLHLLPKLHSLLLVGSVLGFVLMQFVAIGAMEWRS-- 317
           LGK MKKKKE++GFLL+NSEEIGYLHLLPKL+SL+LVGSVLGFVL+QFVAIGAMEWRS  
Sbjct: 181 LGKIMKKKKEVIGFLLENSEEIGYLHLLPKLYSLVLVGSVLGFVLIQFVAIGAMEWRSSN 240

Query: 318 NALSGLNCVEKLIGILFLSVNSRHSGESIVDLSRLSSAILLLFVAMMYLPPYTSFMPIKI 377
           N  SGLN VEKLIGILFLSVNSRHSGES VDLS LSSAIL+LFVAMMYLPPYTSFMPIK 
Sbjct: 241 NGFSGLNWVEKLIGILFLSVNSRHSGESTVDLSTLSSAILVLFVAMMYLPPYTSFMPIK- 300

Query: 378 DEGEEEENQLLEANKRSRKIKRAKFMDNFVFSQLFYLIIFIFIICITERHNIKEDPLNFN 437
              EEEEN++    KR  + KRAKFMDNFVFSQL YLIIF FIICITERHN+K+DPLNFN
Sbjct: 301 -SIEEEENEMEGNRKRRSRTKRAKFMDNFVFSQLSYLIIFTFIICITERHNMKQDPLNFN 360

Query: 438 IFNIIFEVISAYGNVGFTMGYNCKRQIHPQSNCADKSFGFSGKWSDNGKVVLIVVMIFGR 497
           IFNI+FEVISAYGNVGF+MGY+C R++HPQSNC DKSFGFSGKWSDNGK+VLIVVMIFGR
Sbjct: 361 IFNIVFEVISAYGNVGFSMGYSCGRRVHPQSNCVDKSFGFSGKWSDNGKLVLIVVMIFGR 420

Query: 498 LKKFNMNK-QRPWKLL 507
           LKKFNMN+ QR WKLL
Sbjct: 421 LKKFNMNRSQRAWKLL 431