PI0002705.1 (mRNA) Melon (PI 482460) v1

Overview
NamePI0002705.1
TypemRNA
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionBOI-related E3 ubiquitin-protein ligase 1
Locationchr03: 110415 .. 113643 (-)
Sequence length1702
RNA-Seq ExpressionPI0002705.1
SyntenyPI0002705.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CACCGATTCATTCATTTCATTCTAATAAATTTTCCACTCCCACCGCACCACCTCCCTACAATTCCTCTTCCTCTCTCTCCTATAATTCTTCATCTTCATCTTCATCTTCATCGTCTTCTTCTTCTTCTTCTTCTTCCTCATCCTTTTCGTCGGTTACCTTACCTCTCCAGAACCCTCCATCCATCTTTTCCCTTTCCCCTCTCTCTCTCTCTCTCTCTCTCCGTGTATATATATTTCTAGATCCTATTCCTCCTCCTTCATCATTTGATTCTGCATATACAATCTTCAAGGTATTTCTATCTTTTCTTTCTCTCTCTCTCTCTCTATATATTTTTTTTCTTCTTCTTTTCGCTATTACTATGTGTATATATGTATTGCTTGTGAATTATGTGGATAAATCTGTGTGGATTGTCGAATGGCAGGATCTGGAGATGATATTTCTTCTCATCTCATGCTGCCCAAATGACTTCTTCTTAAGGTAATCCTTTTATTCCCTTACTTTTACGTTTGCATCGTTGCTAAAGTCGTGCCTCATAAGGAAAACTTCCTACCATGATCTCAACTAAACATTGTTCGTTGTCAAAATTGTTTGATTGCCTGTTCCTTCATTATGCGATTCCGGTCAAACCTGCTCATCAATCTCCCTAACTCTCCTCATTTAGATATCCTCACTTCTGATACGCTAAGCCTTTTCCCATTCCTTTTGCCGCGTCCCCATCCCTGCTGATGTTTTTTCTATCTCTATATGGTTTTATGATTGATTCAACTCCTGCTATTTCTTCTGATCTGATCTTCCTCTAGTGTTATTTCTGAATTCCTTCTGACTATCTCTATAGAATTCAAGGATTCCCTTGAATTTTATGCTTATGTTTATGTTTGACTTTCTAACGAAAACGTTATGGTGGGTAGCTTCTCGTATTTTCACCTAATTGGATGGTTTGTTACTCATAGTAAATGCTACGGATAACTGGATTTTATTGGAATATTGATTGCCCATTATGGGGTTTCAAATGCTGAATTCAGTCCTGGAATATCATACCCATGTTTCGTAATAGGATTTGTTGAAGATGAGCCCCCCTTTTGTTTTATTAACAATGAAGCTTTTGTGGCAATGCGAAGTCTGTTCAATAACTTAATTCATCCAAGATTTTTGACAGTTTTGGGAACCGACTCACAGAGTACTCTAAGCCTTGTTTCTTGTTTGTCTAGTGTAATGCTAAAGTAACCATAGCTCAAGGTTAATTGACATATGAATTTTTTCATTTGAGGTTGGAGCTTCATATCTTCATACTCCACTTGTACTTAAAAATTTATATTATTGTCCTTTTTCTTCCTCGTGGTTTTTTTCCATCAGTTAAATAGAATTATTAAGACTGCGGTCTCCTTTTGCAGGCAGTGAACCTTCCATGCTGTCGGTATTTTAATTTTCTCTTTATCTGAAGTTCATAGATTCAATTCTTTGGAAAATGTTGGGTGGCAACAATGGAAATGGTTTGCTTCCCGTTTTCCTTGATGATAATCGAACCCAGTATCAAAATAATGCTTCTGGTCAGCTGCAGTTATTTGGGAATTGTGAGTTTTTTTCTCTCTAAAATATCTGATTTCAACTTACTGCATTCCTTAATTTTTTATTTATTAGTATGTCTAGCCCAAGTAAATTGATGTGAATAACAATGCAAGTCTGATCGGTATTTGGAAGATTAATCAGATCTTGTTAAATGTTATTTGGTTGTCCCGTCTCATAGAATTCACAATTTATGTTGTAGTGCCCGTTAGTTGTGGAATCGAGCCTGTAAATTATTTTGGAAACGAGCAAATGGTTCATATGCTTCGCCCTAACAAAAGAAGTAGGGAAATAGAAGATTTCTCAAGACAGCAGAAGCTTCAGATTTCCCTAAATTATGCTTGTCAAGATGACGCTGATCGTTCAACAAGCATTAGAAACCCAAATGCTGTATCAACAGGCTTAAGGCTATCATATGATGATGATGAACACAATTCCTCTGTTACCACTGCCAGTGGAAGTATAACAGCTGCACCTTCAATCATTTTTTCACTTGGTGATAATATCAGGACTGAGATTGATAGACAAAAGGAAGAATTTGATCAATATATTAAACTTCAGGTACTTTAATCTCCTCAGAGCTTCTAGGACATTCTGCTCTTCTGGATTTTAATAAGAGTGCATGCTGCATAGGGATAAAACTAAAATAATTTGTGATATTATTTCCTGGAAGATGTTTGCGATTGTAGCCTAATTAGTTCATTAAGAGCTTCTTATTACTCTTTATAGCAAAATTACAATCACTTCACGCGATGCCTGTAAATGGGGTGCTCATGCTAAAGTATCCTTCAATGCGGTTGATTCAATTGCATCTAATGTGTTAGATTTTCCATCTGCCTGGCAAACAGGAAGAACATTTGGCAAAGGGTATTAGGGACATGAAGCAAAGACATATGGCTTCCTTTCTTAGTGCGATTGAGAAAGGCATCGGAAAGAAACTACATGAAAAGGATGTTGAAATTGAGAGCATGAACCGTAAAAACAGAGAGCTAGTGGAGAGAATAAAGCATGTAGCCACGGAAGCTCAAAATTGGCATTGCAGAGCAAAGTACAATGAGTCAGTCGTAAATGTCCTGAAAAACAATCTTCAGCATGCTATCTCGCAGGGTGCCGACCAAGCGAAGGAAGGGTTTGGAGATAGTGAAGTGGATGATGCTGCCTCAAACATTGATCCAAACAATTACCCACGTGTTCCAGGTGGGACAATAAAACCTTCAACCAGCATGGAACACATGATTTGCAGAGCATGCAAGGCAAAAGAGGTTTCCATCCTACTTATGCCTTGTAGACATTTATGTTTGTGTAAAGATTGCGATGGACTTGTAAATGTCTGTCCTGTCTGTCACTTGATCAAAACGGCTGGTATCCAAGTCTACTTGTCCTAAATTAAAAATCTTACCAAAGTGCAAAACAAAATCTCGTTTTCCTTCTGTCTGTATGATATATACCTGCATTTGTTTTTAATCACTAAGGTGAACGTTGAAGTTGCTCATTTGAAAGTTGGGTTTATCGTACTCGTCTTAGCATGAAGAACAATTACATTCTCTTTTCATGGCCCACGTTTCTGGTCTTCTGGTTGATAGATCATTTATTGATTGTTGTCGTTAAGATTTTTTGAACATGCATTTCGAGCAGAAGTTCCAATTCAATCCAACTCACAGTACT

mRNA sequence

CACCGATTCATTCATTTCATTCTAATAAATTTTCCACTCCCACCGCACCACCTCCCTACAATTCCTCTTCCTCTCTCTCCTATAATTCTTCATCTTCATCTTCATCTTCATCGTCTTCTTCTTCTTCTTCTTCTTCCTCATCCTTTTCGTCGGTTACCTTACCTCTCCAGAACCCTCCATCCATCTTTTCCCTTTCCCCTCTCTCTCTCTCTCTCTCTCTCCGTGTATATATATTTCTAGATCCTATTCCTCCTCCTTCATCATTTGATTCTGCATATACAATCTTCAAGGATCTGGAGATGATATTTCTTCTCATCTCATGCTGCCCAAATGACTTCTTCTTAAGGCAGTGAACCTTCCATGCTGTCGGTATTTTAATTTTCTCTTTATCTGAAGTTCATAGATTCAATTCTTTGGAAAATGTTGGGTGGCAACAATGGAAATGGTTTGCTTCCCGTTTTCCTTGATGATAATCGAACCCAGTATCAAAATAATGCTTCTGGTCAGCTGCAGTTATTTGGGAATTTGCCCGTTAGTTGTGGAATCGAGCCTGTAAATTATTTTGGAAACGAGCAAATGGTTCATATGCTTCGCCCTAACAAAAGAAGTAGGGAAATAGAAGATTTCTCAAGACAGCAGAAGCTTCAGATTTCCCTAAATTATGCTTGTCAAGATGACGCTGATCGTTCAACAAGCATTAGAAACCCAAATGCTGTATCAACAGGCTTAAGGCTATCATATGATGATGATGAACACAATTCCTCTGTTACCACTGCCAGTGGAAGTATAACAGCTGCACCTTCAATCATTTTTTCACTTGGTGATAATATCAGGACTGAGATTGATAGACAAAAGGAAGAATTTGATCAATATATTAAACTTCAGGAAGAACATTTGGCAAAGGGTATTAGGGACATGAAGCAAAGACATATGGCTTCCTTTCTTAGTGCGATTGAGAAAGGCATCGGAAAGAAACTACATGAAAAGGATGTTGAAATTGAGAGCATGAACCGTAAAAACAGAGAGCTAGTGGAGAGAATAAAGCATGTAGCCACGGAAGCTCAAAATTGGCATTGCAGAGCAAAGTACAATGAGTCAGTCGTAAATGTCCTGAAAAACAATCTTCAGCATGCTATCTCGCAGGGTGCCGACCAAGCGAAGGAAGGGTTTGGAGATAGTGAAGTGGATGATGCTGCCTCAAACATTGATCCAAACAATTACCCACGTGTTCCAGGTGGGACAATAAAACCTTCAACCAGCATGGAACACATGATTTGCAGAGCATGCAAGGCAAAAGAGGTTTCCATCCTACTTATGCCTTGTAGACATTTATGTTTGTGTAAAGATTGCGATGGACTTGTAAATGTCTGTCCTGTCTGTCACTTGATCAAAACGGCTGGTATCCAAGTCTACTTGTCCTAAATTAAAAATCTTACCAAAGTGCAAAACAAAATCTCGTTTTCCTTCTGTCTGTATGATATATACCTGCATTTGTTTTTAATCACTAAGGTGAACGTTGAAGTTGCTCATTTGAAAGTTGGGTTTATCGTACTCGTCTTAGCATGAAGAACAATTACATTCTCTTTTCATGGCCCACGTTTCTGGTCTTCTGGTTGATAGATCATTTATTGATTGTTGTCGTTAAGATTTTTTGAACATGCATTTCGAGCAGAAGTTCCAATTCAATCCAACTCACAGTACT

Coding sequence (CDS)

ATGTTGGGTGGCAACAATGGAAATGGTTTGCTTCCCGTTTTCCTTGATGATAATCGAACCCAGTATCAAAATAATGCTTCTGGTCAGCTGCAGTTATTTGGGAATTTGCCCGTTAGTTGTGGAATCGAGCCTGTAAATTATTTTGGAAACGAGCAAATGGTTCATATGCTTCGCCCTAACAAAAGAAGTAGGGAAATAGAAGATTTCTCAAGACAGCAGAAGCTTCAGATTTCCCTAAATTATGCTTGTCAAGATGACGCTGATCGTTCAACAAGCATTAGAAACCCAAATGCTGTATCAACAGGCTTAAGGCTATCATATGATGATGATGAACACAATTCCTCTGTTACCACTGCCAGTGGAAGTATAACAGCTGCACCTTCAATCATTTTTTCACTTGGTGATAATATCAGGACTGAGATTGATAGACAAAAGGAAGAATTTGATCAATATATTAAACTTCAGGAAGAACATTTGGCAAAGGGTATTAGGGACATGAAGCAAAGACATATGGCTTCCTTTCTTAGTGCGATTGAGAAAGGCATCGGAAAGAAACTACATGAAAAGGATGTTGAAATTGAGAGCATGAACCGTAAAAACAGAGAGCTAGTGGAGAGAATAAAGCATGTAGCCACGGAAGCTCAAAATTGGCATTGCAGAGCAAAGTACAATGAGTCAGTCGTAAATGTCCTGAAAAACAATCTTCAGCATGCTATCTCGCAGGGTGCCGACCAAGCGAAGGAAGGGTTTGGAGATAGTGAAGTGGATGATGCTGCCTCAAACATTGATCCAAACAATTACCCACGTGTTCCAGGTGGGACAATAAAACCTTCAACCAGCATGGAACACATGATTTGCAGAGCATGCAAGGCAAAAGAGGTTTCCATCCTACTTATGCCTTGTAGACATTTATGTTTGTGTAAAGATTGCGATGGACTTGTAAATGTCTGTCCTGTCTGTCACTTGATCAAAACGGCTGGTATCCAAGTCTACTTGTCCTAA

Protein sequence

MLGGNNGNGLLPVFLDDNRTQYQNNASGQLQLFGNLPVSCGIEPVNYFGNEQMVHMLRPNKRSREIEDFSRQQKLQISLNYACQDDADRSTSIRNPNAVSTGLRLSYDDDEHNSSVTTASGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKLQEEHLAKGIRDMKQRHMASFLSAIEKGIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESVVNVLKNNLQHAISQGADQAKEGFGDSEVDDAASNIDPNNYPRVPGGTIKPSTSMEHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCHLIKTAGIQVYLS
Homology
BLAST of PI0002705.1 vs. ExPASy Swiss-Prot
Match: F4IDI6 (Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=3702 GN=BRG2 PE=1 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 9.8e-21
Identity = 75/243 (30.86%), Postives = 124/243 (51.03%), Query Frame = 0

Query: 114 SSVTTASGSITAAPSIIFSLGD--NIRTEIDRQKEEFDQYIKL---QEEHLAKGIRDMKQ 173
           SS    + + T   ++   LG   +I + +++Q+ E D+++ L   Q E +   I + ++
Sbjct: 120 SSSCVNAATTTTTTTLFSFLGQDIDISSHMNQQQHEIDRFVSLHLYQMERVKYEIEEKRK 179

Query: 174 RHMASFLSAIEKGIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESVV 233
           R   + + AIE+G+ K+L  K+ E E + + N  L ER+K ++ E Q W   A+ NE+  
Sbjct: 180 RQARTIMEAIEQGLVKRLRVKEEERERIGKVNHALEERVKSLSIENQIWRDLAQTNEATA 239

Query: 234 NVLKNNLQHAISQGADQAKEGFG----DSEVDDAASNIDPNNYPRVPGG---TIKPSTSM 293
           N L+ NL+H ++Q  D ++ G G     +E DDA S    +      GG   T++    +
Sbjct: 240 NHLRTNLEHVLAQVKDVSR-GAGLEKNMNEEDDAESCCGSS----CGGGGEETVRRRVGL 299

Query: 294 E-----------HMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCHLIKTAGIQV 334
           E             +CR C  +E  +LL+PCRHLCLC  C   V+ CP+C   K A + V
Sbjct: 300 EREAQDKAERRRRRMCRNCGEEESCVLLLPCRHLCLCGVCGSSVHTCPICTSPKNASVHV 357

BLAST of PI0002705.1 vs. ExPASy Swiss-Prot
Match: Q9LDD1 (Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=3702 GN=BRG3 PE=1 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 2.4e-19
Identity = 63/210 (30.00%), Postives = 105/210 (50.00%), Query Frame = 0

Query: 133 LGDNIRTEIDRQKEEFDQYIKLQEEHLAKGIRDMKQRHMASFLSAIEKGIGKKLHEKDVE 192
           LG ++ + + +   + D+ I    E +   I + ++      + A+E+G+ K L  KD E
Sbjct: 126 LGQDLSSNVQQHHFDIDRLISNHVERMRMEIEEKRKTQGRRIVEAVEQGLMKTLRAKDDE 185

Query: 193 IESMNRKNRELVERIKHVATEAQNWHCRAKYNESVVNVLKNNLQHAISQGADQAKEGFGD 252
           I  + + N  L E++K +  E Q W   A+ NE+ VN L++NLQ  ++   ++ +     
Sbjct: 186 INHIGKLNLFLEEKVKSLCVENQIWRDVAQSNEATVNALRSNLQQVLA-AVERNRWEEPP 245

Query: 253 SEVDDAASNIDPNNYP-------RVPGGT--IKPSTSMEHMICRACKAKEVSILLMPCRH 312
           +  DDA S    N+         ++ G     K    +   +CR+C   E S+LL+PCRH
Sbjct: 246 TVADDAQSCCGSNDEGDSEEERWKLAGEAQDTKKMCRVGMSMCRSCGKGEASVLLLPCRH 305

Query: 313 LCLCKDCDGLVNVCPVCHLIKTAGIQVYLS 334
           +CLC  C   +N CP+C   KTA + V LS
Sbjct: 306 MCLCSVCGSSLNTCPICKSPKTASLHVNLS 334

BLAST of PI0002705.1 vs. ExPASy Swiss-Prot
Match: Q9FHE4 (BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG1 PE=1 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 2.0e-18
Identity = 77/250 (30.80%), Postives = 117/250 (46.80%), Query Frame = 0

Query: 88  DRSTSIRNPNAVSTGLRLSYDDDEHNSSVTTASGSITAAPSIIFSLGD-NIRTEIDRQKE 147
           D   S     +  T +     D   +S    AS    A      SL D  + ++I +Q  
Sbjct: 52  DPINSFNKSESELTAISKRQRDSTFDSDALIASQKRRAIAFSPASLIDAELVSQIQQQNS 111

Query: 148 EFDQYIKLQEEHLAKGIRDMKQRHMASFLSAIEKGIGKKLHEKDVEIESMNRKNRELVER 207
           E D+++  Q E L   +   ++       SA++  I KKL  KD EI  M + N  L ER
Sbjct: 112 EIDRFVAQQTETLRIELEARQRTQTRMLASAVQNAILKKLKAKDEEIIRMGKLNWVLQER 171

Query: 208 IKHVATEAQNWHCRAKYNESVVNVLKNNLQHAISQGAD-QAKEGFGDSEVDDAASNIDPN 267
           +K++  E Q W   A+ NE+  N L++NL+  ++Q  D  A       E DDA S+    
Sbjct: 172 VKNLYVENQIWRDLAQTNEATANNLRSNLEQVLAQVDDLDAFRRPLVEEADDAESSCG-- 231

Query: 268 NYPRVPGGTIKPSTSMEHMICRACKAKEVSILLMPCRHLCLCKDC--DGLVNVCPVCHLI 327
                 GG +   T++ +  C+ C     S+L++PCRHLCLC  C    L+  CPVC ++
Sbjct: 232 ---SCDGGDV---TAVVNGGCKRCGELTASVLVLPCRHLCLCTVCGSSALLRTCPVCDMV 291

Query: 328 KTAGIQVYLS 334
            TA + V +S
Sbjct: 292 MTASVHVNMS 293

BLAST of PI0002705.1 vs. ExPASy Swiss-Prot
Match: O81851 (E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 5.9e-18
Identity = 74/244 (30.33%), Postives = 114/244 (46.72%), Query Frame = 0

Query: 99  VSTGLRLSYDDDEHNSSVTTASGSI-TAAPSII-FSLGDNIRTEIDRQKEEFDQYIKLQE 158
           +ST  +  +   + N+ V   S +  +++PS+I   L   I+   ++Q+ E D+++  Q 
Sbjct: 75  LSTKRQRDFQFSDSNAPVKRRSVAFDSSSPSLINVELVSQIQ---NQQQSEIDRFVAQQT 134

Query: 159 EHLAKGIRDMKQRHMASFLSAIEKGIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQN 218
           E L   I   +Q       SA++  I KKL EKD EI  +   N  L ER+K +  E Q 
Sbjct: 135 EKLRIEIEARQQTQTRMLASAVQNVIAKKLKEKDDEIVRIRNLNWVLQERVKSLYVENQI 194

Query: 219 WHCRAKYNESVVNVLKNNLQHAISQ-----GADQAKEGFGDSEVDDAASNIDPNNYPRVP 278
           W   A+ NE+  N L+ NL   ++Q      A    E   +S       +        V 
Sbjct: 195 WRDIAQTNEANANTLRTNLDQVLAQLETFPTASAVVEDDAESSCGSCCGDGGGEAVTAVG 254

Query: 279 GGTIKPSTSMEHMICRACKAKEVSILLMPCRHLCLCKDCDG--LVNVCPVCHLIKTAGIQ 334
           GG            C+ C  +E S+L++PCRHLCLC  C G  L+  CPVC ++  A + 
Sbjct: 255 GG------------CKRCGEREASVLVLPCRHLCLCTVCGGSALLRTCPVCDMVMNASVH 303

BLAST of PI0002705.1 vs. ExPASy Swiss-Prot
Match: Q13489 (Baculoviral IAP repeat-containing protein 3 OS=Homo sapiens OX=9606 GN=BIRC3 PE=1 SV=2)

HSP 1 Score: 51.2 bits (121), Expect = 2.6e-05
Identity = 21/52 (40.38%), Postives = 30/52 (57.69%), Query Frame = 0

Query: 282 EHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCHLIKTAGIQVYLS 334
           E   C+ C  KEVSI+ +PC HL +CKDC   +  CP+C       ++ +LS
Sbjct: 553 EERTCKVCMDKEVSIVFIPCGHLVVCKDCAPSLRKCPICRSTIKGTVRTFLS 604

BLAST of PI0002705.1 vs. ExPASy TrEMBL
Match: A0A1S3B9M9 (BOI-related E3 ubiquitin-protein ligase 1 OS=Cucumis melo OX=3656 GN=LOC103487699 PE=4 SV=1)

HSP 1 Score: 642.5 bits (1656), Expect = 9.7e-181
Identity = 322/333 (96.70%), Postives = 325/333 (97.60%), Query Frame = 0

Query: 1   MLGGNNGNGLLPVFLDDNRTQYQNNASGQLQLFGNLPVSCGIEPVNYFGNEQMVHMLRPN 60
           MLGGNNGNGLLPVFLD+ RTQYQ NASGQLQLFGNLPVSCGIEPVNYFGNEQMVHMLRPN
Sbjct: 1   MLGGNNGNGLLPVFLDEKRTQYQTNASGQLQLFGNLPVSCGIEPVNYFGNEQMVHMLRPN 60

Query: 61  KRSREIEDFSRQQKLQISLNYACQDDADRSTSIRNPNAVSTGLRLSYDDDEHNSSVTTAS 120
           KRSREIEDFSRQQKLQISLNYACQDDADRST+IRNPN VSTGLRLSYDDDEHNSSVTTAS
Sbjct: 61  KRSREIEDFSRQQKLQISLNYACQDDADRSTTIRNPNPVSTGLRLSYDDDEHNSSVTTAS 120

Query: 121 GSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKLQEEHLAKGIRDMKQRHMASFLSAIEK 180
           GSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKLQEEHLAKGIRDMKQRH ASFLSAIEK
Sbjct: 121 GSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKLQEEHLAKGIRDMKQRHTASFLSAIEK 180

Query: 181 GIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESVVNVLKNNLQHAIS 240
           GIG KLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESVVNVLKNNLQHAIS
Sbjct: 181 GIGNKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESVVNVLKNNLQHAIS 240

Query: 241 QGADQAKEGFGDSEVDDAASNIDPNNYPRVPGGTIKPSTSMEHMICRACKAKEVSILLMP 300
           QGADQAKEGFGDSEVDDAASNIDPNNY  VPGGTIKPSTS E+MICRACKAKEVSILLMP
Sbjct: 241 QGADQAKEGFGDSEVDDAASNIDPNNYLSVPGGTIKPSTSKEYMICRACKAKEVSILLMP 300

Query: 301 CRHLCLCKDCDGLVNVCPVCHLIKTAGIQVYLS 334
           CRHLCLCKDCDGLVNVCPVCHLIKTAGIQVYLS
Sbjct: 301 CRHLCLCKDCDGLVNVCPVCHLIKTAGIQVYLS 333

BLAST of PI0002705.1 vs. ExPASy TrEMBL
Match: A0A0A0LNB0 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G348840 PE=4 SV=1)

HSP 1 Score: 640.6 bits (1651), Expect = 3.7e-180
Identity = 320/333 (96.10%), Postives = 326/333 (97.90%), Query Frame = 0

Query: 1   MLGGNNGNGLLPVFLDDNRTQYQNNASGQLQLFGNLPVSCGIEPVNYFGNEQMVHMLRPN 60
           MLGGNNGNGLLPVFLD+ RTQYQNNASGQLQLFGNLPVSCGIEPVNYFGNEQMV MLRPN
Sbjct: 1   MLGGNNGNGLLPVFLDEKRTQYQNNASGQLQLFGNLPVSCGIEPVNYFGNEQMVPMLRPN 60

Query: 61  KRSREIEDFSRQQKLQISLNYACQDDADRSTSIRNPNAVSTGLRLSYDDDEHNSSVTTAS 120
           KRSRE EDFSRQQKLQISLNYACQDDA+RSTSIRNPNAVSTGLRLSYDDDEHNSSVTTAS
Sbjct: 61  KRSRETEDFSRQQKLQISLNYACQDDANRSTSIRNPNAVSTGLRLSYDDDEHNSSVTTAS 120

Query: 121 GSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKLQEEHLAKGIRDMKQRHMASFLSAIEK 180
           GSITAAPSIIFSLGDNIRTE+DRQKEEFDQYIK+QEEHLAKGIRDMKQRHMASFLSA+EK
Sbjct: 121 GSITAAPSIIFSLGDNIRTEVDRQKEEFDQYIKIQEEHLAKGIRDMKQRHMASFLSAVEK 180

Query: 181 GIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESVVNVLKNNLQHAIS 240
           GI KKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESVVNVLKNNLQHAIS
Sbjct: 181 GIEKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESVVNVLKNNLQHAIS 240

Query: 241 QGADQAKEGFGDSEVDDAASNIDPNNYPRVPGGTIKPSTSMEHMICRACKAKEVSILLMP 300
           QGADQAKEGFGDSEVDDAASNIDPNNY  VPGGTIKPSTS E+MICRACKAKEVSILLMP
Sbjct: 241 QGADQAKEGFGDSEVDDAASNIDPNNYVNVPGGTIKPSTSKEYMICRACKAKEVSILLMP 300

Query: 301 CRHLCLCKDCDGLVNVCPVCHLIKTAGIQVYLS 334
           CRHLCLCKDCDGLVNVCPVCHLIKTAGIQVYLS
Sbjct: 301 CRHLCLCKDCDGLVNVCPVCHLIKTAGIQVYLS 333

BLAST of PI0002705.1 vs. ExPASy TrEMBL
Match: A0A5A7V171 (BOI-related E3 ubiquitin-protein ligase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G002200 PE=4 SV=1)

HSP 1 Score: 619.4 bits (1596), Expect = 8.8e-174
Identity = 322/382 (84.29%), Postives = 325/382 (85.08%), Query Frame = 0

Query: 1   MLGGNNGNGLLPVFLDDNRTQYQNNASGQLQLFGNLPVSCGIEPVNYFGNEQMVHMLRPN 60
           MLGGNNGNGLLPVFLD+ RTQYQ NASGQLQLFGNLPVSCGIEPVNYFGNEQMVHMLRPN
Sbjct: 1   MLGGNNGNGLLPVFLDEKRTQYQTNASGQLQLFGNLPVSCGIEPVNYFGNEQMVHMLRPN 60

Query: 61  KRSREIEDFSRQQKLQISLNYACQDDADRSTSIRNPNAVSTGLRLSYDDDEHNSSVTTAS 120
           KRSREIEDFSRQQKLQISLNYACQDDADRST+IRNPN VSTGLRLSYDDDEHNSSVTTAS
Sbjct: 61  KRSREIEDFSRQQKLQISLNYACQDDADRSTTIRNPNPVSTGLRLSYDDDEHNSSVTTAS 120

Query: 121 GSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKL-------------------------- 180
           GSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKL                          
Sbjct: 121 GSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKLQFIKSFLLLFIAITITSRDAVNGVLM 180

Query: 181 -----------------------QEEHLAKGIRDMKQRHMASFLSAIEKGIGKKLHEKDV 240
                                  QEEHLAKGIRDMKQRH ASFLSAIEKGIG KLHEKDV
Sbjct: 181 LKYPSMRLIQLGLMCWIFHLPGKQEEHLAKGIRDMKQRHTASFLSAIEKGIGNKLHEKDV 240

Query: 241 EIESMNRKNRELVERIKHVATEAQNWHCRAKYNESVVNVLKNNLQHAISQGADQAKEGFG 300
           EIESMNRKNRELVERIKHVATEAQNWHCRAKYNESVVNVLKNNLQHAISQGADQAKEGFG
Sbjct: 241 EIESMNRKNRELVERIKHVATEAQNWHCRAKYNESVVNVLKNNLQHAISQGADQAKEGFG 300

Query: 301 DSEVDDAASNIDPNNYPRVPGGTIKPSTSMEHMICRACKAKEVSILLMPCRHLCLCKDCD 334
           DSEVDDAASNIDPNNY  VPGGTIKPSTS E+MICRACKAKEVSILLMPCRHLCLCKDCD
Sbjct: 301 DSEVDDAASNIDPNNYLSVPGGTIKPSTSKEYMICRACKAKEVSILLMPCRHLCLCKDCD 360

BLAST of PI0002705.1 vs. ExPASy TrEMBL
Match: A0A6J1KC06 (BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111492498 PE=4 SV=1)

HSP 1 Score: 605.1 bits (1559), Expect = 1.7e-169
Identity = 308/339 (90.86%), Postives = 319/339 (94.10%), Query Frame = 0

Query: 1   MLGGNNGNGLLPVFLDDNRTQYQNNASGQLQLFGNLPVSCGIEPVNYFGNEQMVHMLRPN 60
           MLGGNNGNGLLPVF D+NR QYQ NASGQLQLFG++PVSCGIEPVNYFG+EQ+ HMLRPN
Sbjct: 1   MLGGNNGNGLLPVFQDENRNQYQTNASGQLQLFGSVPVSCGIEPVNYFGSEQLAHMLRPN 60

Query: 61  KRSREIEDFSRQQKLQISLNY-ACQDDADRSTSIRNPNAVSTGLRLSYDDDEHNSSVTTA 120
           KRSREIEDFS QQKLQISLNY ACQDDADRS SIRNPNAVSTGLRLSYDDDEHNSSVTTA
Sbjct: 61  KRSREIEDFSWQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTA 120

Query: 121 SGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKLQEEHLAKGIRDMKQRHMASFLSAIE 180
           SGS+ AAPSIIFSLGDNIRTEIDRQKEEFDQYIK+QEEHLAKGIRDMKQRHMASFLSAIE
Sbjct: 121 SGSMAAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHMASFLSAIE 180

Query: 181 KGIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESVVNVLKNNLQHAI 240
           KGIGKKLHEKDVEIESMNRKNRELVERIK VATEAQNWHCRAKYNESVVNVLKNNLQHAI
Sbjct: 181 KGIGKKLHEKDVEIESMNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKNNLQHAI 240

Query: 241 SQGADQAKEGFGDSEVDDAASNIDPNNYPRVPGGTIKPSTS-----MEHMICRACKAKEV 300
           SQGADQAKEGFGDSEVDDAAS+IDPNNY  V GGT+K STS      EHMIC+ACKAKEV
Sbjct: 241 SQGADQAKEGFGDSEVDDAASHIDPNNYLNVHGGTMKSSTSNLPGFKEHMICKACKAKEV 300

Query: 301 SILLMPCRHLCLCKDCDGLVNVCPVCHLIKTAGIQVYLS 334
           SILLMPCRHLCLC+DCDGLVNVCPVC LIKTAGIQVYLS
Sbjct: 301 SILLMPCRHLCLCRDCDGLVNVCPVCQLIKTAGIQVYLS 339

BLAST of PI0002705.1 vs. ExPASy TrEMBL
Match: A0A6J1HCD7 (BOI-related E3 ubiquitin-protein ligase 1 OS=Cucurbita moschata OX=3662 GN=LOC111462352 PE=4 SV=1)

HSP 1 Score: 602.1 bits (1551), Expect = 1.5e-168
Identity = 306/339 (90.27%), Postives = 318/339 (93.81%), Query Frame = 0

Query: 1   MLGGNNGNGLLPVFLDDNRTQYQNNASGQLQLFGNLPVSCGIEPVNYFGNEQMVHMLRPN 60
           MLGGNNGNGLLPVF D+NR QYQ NASGQLQLFG++PVSCGIEPVNYFG+EQ+ HMLRPN
Sbjct: 1   MLGGNNGNGLLPVFQDENRNQYQTNASGQLQLFGSVPVSCGIEPVNYFGSEQLAHMLRPN 60

Query: 61  KRSREIEDFSRQQKLQISLNY-ACQDDADRSTSIRNPNAVSTGLRLSYDDDEHNSSVTTA 120
           KRSREIEDFS QQKLQISLNY ACQDDADRS SIRNPNAVSTGLRLSYDDDEHNSSVTTA
Sbjct: 61  KRSREIEDFSWQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTA 120

Query: 121 SGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKLQEEHLAKGIRDMKQRHMASFLSAIE 180
           SGS+  APSIIFSLGDNIRTEIDRQKEEFDQYIK+QEEHLAKGIRDMKQRH+ASFLSAIE
Sbjct: 121 SGSMATAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHVASFLSAIE 180

Query: 181 KGIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESVVNVLKNNLQHAI 240
           KGIGKKLHEKDVEIESMNRKNRELVERIK VATEAQNWHCRAKYNESVVNVLKNNLQHAI
Sbjct: 181 KGIGKKLHEKDVEIESMNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKNNLQHAI 240

Query: 241 SQGADQAKEGFGDSEVDDAASNIDPNNYPRVPGGTIKPSTS-----MEHMICRACKAKEV 300
           SQGADQAKEGFGDSEVDDAAS+IDPNNY  V GGT+K STS      EHMIC+ACKAKEV
Sbjct: 241 SQGADQAKEGFGDSEVDDAASHIDPNNYLNVHGGTMKSSTSNLPGFKEHMICKACKAKEV 300

Query: 301 SILLMPCRHLCLCKDCDGLVNVCPVCHLIKTAGIQVYLS 334
           SILLMPCRHLCLC+DCDGLVNVCPVC LIKTAGIQVYLS
Sbjct: 301 SILLMPCRHLCLCRDCDGLVNVCPVCQLIKTAGIQVYLS 339

BLAST of PI0002705.1 vs. NCBI nr
Match: XP_008444346.1 (PREDICTED: BOI-related E3 ubiquitin-protein ligase 1 [Cucumis melo])

HSP 1 Score: 642.5 bits (1656), Expect = 2.0e-180
Identity = 322/333 (96.70%), Postives = 325/333 (97.60%), Query Frame = 0

Query: 1   MLGGNNGNGLLPVFLDDNRTQYQNNASGQLQLFGNLPVSCGIEPVNYFGNEQMVHMLRPN 60
           MLGGNNGNGLLPVFLD+ RTQYQ NASGQLQLFGNLPVSCGIEPVNYFGNEQMVHMLRPN
Sbjct: 1   MLGGNNGNGLLPVFLDEKRTQYQTNASGQLQLFGNLPVSCGIEPVNYFGNEQMVHMLRPN 60

Query: 61  KRSREIEDFSRQQKLQISLNYACQDDADRSTSIRNPNAVSTGLRLSYDDDEHNSSVTTAS 120
           KRSREIEDFSRQQKLQISLNYACQDDADRST+IRNPN VSTGLRLSYDDDEHNSSVTTAS
Sbjct: 61  KRSREIEDFSRQQKLQISLNYACQDDADRSTTIRNPNPVSTGLRLSYDDDEHNSSVTTAS 120

Query: 121 GSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKLQEEHLAKGIRDMKQRHMASFLSAIEK 180
           GSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKLQEEHLAKGIRDMKQRH ASFLSAIEK
Sbjct: 121 GSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKLQEEHLAKGIRDMKQRHTASFLSAIEK 180

Query: 181 GIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESVVNVLKNNLQHAIS 240
           GIG KLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESVVNVLKNNLQHAIS
Sbjct: 181 GIGNKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESVVNVLKNNLQHAIS 240

Query: 241 QGADQAKEGFGDSEVDDAASNIDPNNYPRVPGGTIKPSTSMEHMICRACKAKEVSILLMP 300
           QGADQAKEGFGDSEVDDAASNIDPNNY  VPGGTIKPSTS E+MICRACKAKEVSILLMP
Sbjct: 241 QGADQAKEGFGDSEVDDAASNIDPNNYLSVPGGTIKPSTSKEYMICRACKAKEVSILLMP 300

Query: 301 CRHLCLCKDCDGLVNVCPVCHLIKTAGIQVYLS 334
           CRHLCLCKDCDGLVNVCPVCHLIKTAGIQVYLS
Sbjct: 301 CRHLCLCKDCDGLVNVCPVCHLIKTAGIQVYLS 333

BLAST of PI0002705.1 vs. NCBI nr
Match: XP_011649486.1 (BOI-related E3 ubiquitin-protein ligase 1 [Cucumis sativus] >XP_011649487.1 BOI-related E3 ubiquitin-protein ligase 1 [Cucumis sativus] >KGN62297.1 hypothetical protein Csa_018673 [Cucumis sativus])

HSP 1 Score: 640.6 bits (1651), Expect = 7.6e-180
Identity = 320/333 (96.10%), Postives = 326/333 (97.90%), Query Frame = 0

Query: 1   MLGGNNGNGLLPVFLDDNRTQYQNNASGQLQLFGNLPVSCGIEPVNYFGNEQMVHMLRPN 60
           MLGGNNGNGLLPVFLD+ RTQYQNNASGQLQLFGNLPVSCGIEPVNYFGNEQMV MLRPN
Sbjct: 1   MLGGNNGNGLLPVFLDEKRTQYQNNASGQLQLFGNLPVSCGIEPVNYFGNEQMVPMLRPN 60

Query: 61  KRSREIEDFSRQQKLQISLNYACQDDADRSTSIRNPNAVSTGLRLSYDDDEHNSSVTTAS 120
           KRSRE EDFSRQQKLQISLNYACQDDA+RSTSIRNPNAVSTGLRLSYDDDEHNSSVTTAS
Sbjct: 61  KRSRETEDFSRQQKLQISLNYACQDDANRSTSIRNPNAVSTGLRLSYDDDEHNSSVTTAS 120

Query: 121 GSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKLQEEHLAKGIRDMKQRHMASFLSAIEK 180
           GSITAAPSIIFSLGDNIRTE+DRQKEEFDQYIK+QEEHLAKGIRDMKQRHMASFLSA+EK
Sbjct: 121 GSITAAPSIIFSLGDNIRTEVDRQKEEFDQYIKIQEEHLAKGIRDMKQRHMASFLSAVEK 180

Query: 181 GIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESVVNVLKNNLQHAIS 240
           GI KKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESVVNVLKNNLQHAIS
Sbjct: 181 GIEKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESVVNVLKNNLQHAIS 240

Query: 241 QGADQAKEGFGDSEVDDAASNIDPNNYPRVPGGTIKPSTSMEHMICRACKAKEVSILLMP 300
           QGADQAKEGFGDSEVDDAASNIDPNNY  VPGGTIKPSTS E+MICRACKAKEVSILLMP
Sbjct: 241 QGADQAKEGFGDSEVDDAASNIDPNNYVNVPGGTIKPSTSKEYMICRACKAKEVSILLMP 300

Query: 301 CRHLCLCKDCDGLVNVCPVCHLIKTAGIQVYLS 334
           CRHLCLCKDCDGLVNVCPVCHLIKTAGIQVYLS
Sbjct: 301 CRHLCLCKDCDGLVNVCPVCHLIKTAGIQVYLS 333

BLAST of PI0002705.1 vs. NCBI nr
Match: XP_038884634.1 (probable BOI-related E3 ubiquitin-protein ligase 2 [Benincasa hispida])

HSP 1 Score: 636.7 bits (1641), Expect = 1.1e-178
Identity = 322/338 (95.27%), Postives = 326/338 (96.45%), Query Frame = 0

Query: 1   MLGGNNGNGLLPVFLDDNRTQYQNNASGQLQLFGNLPVSCGIEPVNYFGNEQMVHMLRPN 60
           MLGGNNGNGLLPVFLD+NRTQYQ NASGQLQLFGNLPVSCGIEPVNYFGNEQ+VHMLRPN
Sbjct: 1   MLGGNNGNGLLPVFLDENRTQYQTNASGQLQLFGNLPVSCGIEPVNYFGNEQIVHMLRPN 60

Query: 61  KRSREIEDFSRQQKLQISLNYACQDDADRSTSIRNPNAVSTGLRLSYDDDEHNSSVTTAS 120
           KRSREIEDFSRQQKLQISLNYACQDDADRS SIRNPNAVSTGLRLSYDDDEHNSSVTTAS
Sbjct: 61  KRSREIEDFSRQQKLQISLNYACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTAS 120

Query: 121 GSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKLQEEHLAKGIRDMKQRHMASFLSAIEK 180
           GS+TAAPSII SLGDNIRTEIDRQKEEFDQYIKLQEEHLAKGIRDMKQRHMASFLSAIEK
Sbjct: 121 GSMTAAPSIILSLGDNIRTEIDRQKEEFDQYIKLQEEHLAKGIRDMKQRHMASFLSAIEK 180

Query: 181 GIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESVVNVLKNNLQHAIS 240
           GIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESVVNVLKNNLQHAIS
Sbjct: 181 GIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESVVNVLKNNLQHAIS 240

Query: 241 QGADQAKEGFGDSEVDDAASNIDPNNYPRVPGGTIKPSTS-----MEHMICRACKAKEVS 300
           QGADQAKEGFGDSEVDDAASNIDPNNY  VPGGTIK STS      EHMICRACKAKEVS
Sbjct: 241 QGADQAKEGFGDSEVDDAASNIDPNNYLSVPGGTIKSSTSNLPGFKEHMICRACKAKEVS 300

Query: 301 ILLMPCRHLCLCKDCDGLVNVCPVCHLIKTAGIQVYLS 334
           ILLMPCRHLCLCKDCDGLVNVCPVCHLIKTAGIQV+LS
Sbjct: 301 ILLMPCRHLCLCKDCDGLVNVCPVCHLIKTAGIQVFLS 338

BLAST of PI0002705.1 vs. NCBI nr
Match: KAA0061080.1 (BOI-related E3 ubiquitin-protein ligase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 619.4 bits (1596), Expect = 1.8e-173
Identity = 322/382 (84.29%), Postives = 325/382 (85.08%), Query Frame = 0

Query: 1   MLGGNNGNGLLPVFLDDNRTQYQNNASGQLQLFGNLPVSCGIEPVNYFGNEQMVHMLRPN 60
           MLGGNNGNGLLPVFLD+ RTQYQ NASGQLQLFGNLPVSCGIEPVNYFGNEQMVHMLRPN
Sbjct: 1   MLGGNNGNGLLPVFLDEKRTQYQTNASGQLQLFGNLPVSCGIEPVNYFGNEQMVHMLRPN 60

Query: 61  KRSREIEDFSRQQKLQISLNYACQDDADRSTSIRNPNAVSTGLRLSYDDDEHNSSVTTAS 120
           KRSREIEDFSRQQKLQISLNYACQDDADRST+IRNPN VSTGLRLSYDDDEHNSSVTTAS
Sbjct: 61  KRSREIEDFSRQQKLQISLNYACQDDADRSTTIRNPNPVSTGLRLSYDDDEHNSSVTTAS 120

Query: 121 GSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKL-------------------------- 180
           GSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKL                          
Sbjct: 121 GSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKLQFIKSFLLLFIAITITSRDAVNGVLM 180

Query: 181 -----------------------QEEHLAKGIRDMKQRHMASFLSAIEKGIGKKLHEKDV 240
                                  QEEHLAKGIRDMKQRH ASFLSAIEKGIG KLHEKDV
Sbjct: 181 LKYPSMRLIQLGLMCWIFHLPGKQEEHLAKGIRDMKQRHTASFLSAIEKGIGNKLHEKDV 240

Query: 241 EIESMNRKNRELVERIKHVATEAQNWHCRAKYNESVVNVLKNNLQHAISQGADQAKEGFG 300
           EIESMNRKNRELVERIKHVATEAQNWHCRAKYNESVVNVLKNNLQHAISQGADQAKEGFG
Sbjct: 241 EIESMNRKNRELVERIKHVATEAQNWHCRAKYNESVVNVLKNNLQHAISQGADQAKEGFG 300

Query: 301 DSEVDDAASNIDPNNYPRVPGGTIKPSTSMEHMICRACKAKEVSILLMPCRHLCLCKDCD 334
           DSEVDDAASNIDPNNY  VPGGTIKPSTS E+MICRACKAKEVSILLMPCRHLCLCKDCD
Sbjct: 301 DSEVDDAASNIDPNNYLSVPGGTIKPSTSKEYMICRACKAKEVSILLMPCRHLCLCKDCD 360

BLAST of PI0002705.1 vs. NCBI nr
Match: XP_022997618.1 (BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita maxima] >XP_022997619.1 BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita maxima] >XP_023545645.1 BOI-related E3 ubiquitin-protein ligase 1 [Cucurbita pepo subsp. pepo] >XP_023545646.1 BOI-related E3 ubiquitin-protein ligase 1 [Cucurbita pepo subsp. pepo] >XP_023545647.1 BOI-related E3 ubiquitin-protein ligase 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 605.1 bits (1559), Expect = 3.6e-169
Identity = 308/339 (90.86%), Postives = 319/339 (94.10%), Query Frame = 0

Query: 1   MLGGNNGNGLLPVFLDDNRTQYQNNASGQLQLFGNLPVSCGIEPVNYFGNEQMVHMLRPN 60
           MLGGNNGNGLLPVF D+NR QYQ NASGQLQLFG++PVSCGIEPVNYFG+EQ+ HMLRPN
Sbjct: 1   MLGGNNGNGLLPVFQDENRNQYQTNASGQLQLFGSVPVSCGIEPVNYFGSEQLAHMLRPN 60

Query: 61  KRSREIEDFSRQQKLQISLNY-ACQDDADRSTSIRNPNAVSTGLRLSYDDDEHNSSVTTA 120
           KRSREIEDFS QQKLQISLNY ACQDDADRS SIRNPNAVSTGLRLSYDDDEHNSSVTTA
Sbjct: 61  KRSREIEDFSWQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTA 120

Query: 121 SGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKLQEEHLAKGIRDMKQRHMASFLSAIE 180
           SGS+ AAPSIIFSLGDNIRTEIDRQKEEFDQYIK+QEEHLAKGIRDMKQRHMASFLSAIE
Sbjct: 121 SGSMAAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHMASFLSAIE 180

Query: 181 KGIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESVVNVLKNNLQHAI 240
           KGIGKKLHEKDVEIESMNRKNRELVERIK VATEAQNWHCRAKYNESVVNVLKNNLQHAI
Sbjct: 181 KGIGKKLHEKDVEIESMNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKNNLQHAI 240

Query: 241 SQGADQAKEGFGDSEVDDAASNIDPNNYPRVPGGTIKPSTS-----MEHMICRACKAKEV 300
           SQGADQAKEGFGDSEVDDAAS+IDPNNY  V GGT+K STS      EHMIC+ACKAKEV
Sbjct: 241 SQGADQAKEGFGDSEVDDAASHIDPNNYLNVHGGTMKSSTSNLPGFKEHMICKACKAKEV 300

Query: 301 SILLMPCRHLCLCKDCDGLVNVCPVCHLIKTAGIQVYLS 334
           SILLMPCRHLCLC+DCDGLVNVCPVC LIKTAGIQVYLS
Sbjct: 301 SILLMPCRHLCLCRDCDGLVNVCPVCQLIKTAGIQVYLS 339

BLAST of PI0002705.1 vs. TAIR 10
Match: AT1G10650.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 371.7 bits (953), Expect = 6.2e-103
Identity = 201/346 (58.09%), Postives = 247/346 (71.39%), Query Frame = 0

Query: 1   MLGGNNGNGLLPVFLDDNRTQYQNN-ASGQLQLFGNLPVSCGIEPVNYFGNEQMVHMLRP 60
           ML GNNGN   PVF+++N+ QYQ N  S QL L GN+   C ++PVNYF N+ +V M+RP
Sbjct: 1   MLSGNNGNTAPPVFMNENQLQYQTNLRSNQLHLLGNMGGGCTVDPVNYFANDNLVPMIRP 60

Query: 61  NKRSREIEDFS-----RQQKLQISLNYACQDDADRSTSIRNPNAVSTGLRLSYDDDEHNS 120
           NKR RE E  S     +QQKLQ+SLNY   + + R   +   N VSTGLRLSYDDDEHNS
Sbjct: 61  NKRGREAESISNNIQRQQQKLQMSLNYNYNNTSVRE-EVPKENLVSTGLRLSYDDDEHNS 120

Query: 121 SVTTASGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKLQEEHLAKGIRDMKQRHMASF 180
           SVT+ASGSI AA  I  SL D++R ++ RQK+EFDQ+IK+Q   +AKG+RDMKQRH+ASF
Sbjct: 121 SVTSASGSILAASPIFQSLDDSLRIDLHRQKDEFDQFIKIQAAQMAKGVRDMKQRHIASF 180

Query: 181 LSAIEKGIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESVVNVLKNN 240
           L+ +EKG+ KKL EKD EI  MN+KN+ELVERIK VA EAQNWH RAKYNESVVNVLK N
Sbjct: 181 LTTLEKGVSKKLQEKDHEINDMNKKNKELVERIKQVAMEAQNWHYRAKYNESVVNVLKAN 240

Query: 241 LQHAISQ------GADQAKEGFGDSEVDDAASN-IDPNNYPRVPGGTIKPSTSMEHMICR 300
           LQ A+S        ADQ KEGFGDSE+DDAAS+ IDPNN      G        + M C+
Sbjct: 241 LQQAMSHNNSVIAAADQGKEGFGDSEIDDAASSYIDPNNNNNNNMGI------HQRMRCK 300

Query: 301 ACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCHLIKTAGIQVYLS 334
            C  KEVS+L++PCRHL LCK+CD    +CPVC  +K++ +QV+ S
Sbjct: 301 MCNVKEVSVLIVPCRHLSLCKECDVFTKICPVCKSLKSSCVQVFFS 339

BLAST of PI0002705.1 vs. TAIR 10
Match: AT1G60610.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 334.7 bits (857), Expect = 8.3e-92
Identity = 187/349 (53.58%), Postives = 237/349 (67.91%), Query Frame = 0

Query: 1   MLGGNNGNGLLPVFLDDNRTQYQNNAS-GQLQLFGNLPVSCGIEPVNYFGNEQMVHMLRP 60
           MLGGNN N +  V ++D++ +YQ+N S  QL L G +   C I+PVNYF N+ +  M+R 
Sbjct: 1   MLGGNNDNPVPQVLMNDSQFRYQSNTSLNQLHLLGTMRAGCTIDPVNYFANDNLAPMMRL 60

Query: 61  NK-RSREIED---FSRQQKLQISLNYAC-QDDADRSTSIRNPNAVSTGLRLSYDDDEHNS 120
           N  R RE E+     RQQKLQISLNY    ++      +   N VSTGLRLSYDDDE NS
Sbjct: 61  NSTRGRETENNNIMQRQQKLQISLNYNYNNNNTVVQDEVPKQNLVSTGLRLSYDDDERNS 120

Query: 121 SVTTASGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKLQEEHLAKGIRDMKQRHMASF 180
           SVT+A+GSIT    +  SLGDNIR +++RQ +E DQ+IK + + +AKG+RD+KQRH+ SF
Sbjct: 121 SVTSANGSITT--PVYQSLGDNIRLDLNRQNDELDQFIKFRADQMAKGVRDIKQRHVTSF 180

Query: 181 LSAIEKGIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESVVNVLKNN 240
           ++A+EK + KKL EKD EIESMN+KNRELV++IK VA EAQNWH +AKYNESVVN LK N
Sbjct: 181 VTALEKDVSKKLQEKDHEIESMNKKNRELVDKIKQVAVEAQNWHYKAKYNESVVNALKVN 240

Query: 241 LQHAISQGAD------------QAKEGFGDSEVDDAASNIDPNNYPRVPGGTIKPSTSME 300
           LQ  +S G D            Q KEGFGDSE+DD A++    NY  +PG    PST M 
Sbjct: 241 LQQVMSHGNDNNAVGGGVADHHQMKEGFGDSEIDDEAASY---NYLNIPG---MPSTGMR 300

Query: 301 HMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCHLIKTAGIQVY 332
              C+ C  K VS+LL+PCRHL LCKDCD    VCPVC  +KT+ +QV+
Sbjct: 301 ---CKLCNVKNVSVLLVPCRHLSLCKDCDVFTGVCPVCQSLKTSSVQVF 338

BLAST of PI0002705.1 vs. TAIR 10
Match: AT1G60610.2 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 334.7 bits (857), Expect = 8.3e-92
Identity = 187/349 (53.58%), Postives = 237/349 (67.91%), Query Frame = 0

Query: 1   MLGGNNGNGLLPVFLDDNRTQYQNNAS-GQLQLFGNLPVSCGIEPVNYFGNEQMVHMLRP 60
           MLGGNN N +  V ++D++ +YQ+N S  QL L G +   C I+PVNYF N+ +  M+R 
Sbjct: 1   MLGGNNDNPVPQVLMNDSQFRYQSNTSLNQLHLLGTMRAGCTIDPVNYFANDNLAPMMRL 60

Query: 61  NK-RSREIED---FSRQQKLQISLNYAC-QDDADRSTSIRNPNAVSTGLRLSYDDDEHNS 120
           N  R RE E+     RQQKLQISLNY    ++      +   N VSTGLRLSYDDDE NS
Sbjct: 61  NSTRGRETENNNIMQRQQKLQISLNYNYNNNNTVVQDEVPKQNLVSTGLRLSYDDDERNS 120

Query: 121 SVTTASGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKLQEEHLAKGIRDMKQRHMASF 180
           SVT+A+GSIT    +  SLGDNIR +++RQ +E DQ+IK + + +AKG+RD+KQRH+ SF
Sbjct: 121 SVTSANGSITT--PVYQSLGDNIRLDLNRQNDELDQFIKFRADQMAKGVRDIKQRHVTSF 180

Query: 181 LSAIEKGIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESVVNVLKNN 240
           ++A+EK + KKL EKD EIESMN+KNRELV++IK VA EAQNWH +AKYNESVVN LK N
Sbjct: 181 VTALEKDVSKKLQEKDHEIESMNKKNRELVDKIKQVAVEAQNWHYKAKYNESVVNALKVN 240

Query: 241 LQHAISQGAD------------QAKEGFGDSEVDDAASNIDPNNYPRVPGGTIKPSTSME 300
           LQ  +S G D            Q KEGFGDSE+DD A++    NY  +PG    PST M 
Sbjct: 241 LQQVMSHGNDNNAVGGGVADHHQMKEGFGDSEIDDEAASY---NYLNIPG---MPSTGMR 300

Query: 301 HMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCHLIKTAGIQVY 332
              C+ C  K VS+LL+PCRHL LCKDCD    VCPVC  +KT+ +QV+
Sbjct: 301 ---CKLCNVKNVSVLLVPCRHLSLCKDCDVFTGVCPVCQSLKTSSVQVF 338

BLAST of PI0002705.1 vs. TAIR 10
Match: AT1G60610.3 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 334.7 bits (857), Expect = 8.3e-92
Identity = 187/349 (53.58%), Postives = 237/349 (67.91%), Query Frame = 0

Query: 1   MLGGNNGNGLLPVFLDDNRTQYQNNAS-GQLQLFGNLPVSCGIEPVNYFGNEQMVHMLRP 60
           MLGGNN N +  V ++D++ +YQ+N S  QL L G +   C I+PVNYF N+ +  M+R 
Sbjct: 1   MLGGNNDNPVPQVLMNDSQFRYQSNTSLNQLHLLGTMRAGCTIDPVNYFANDNLAPMMRL 60

Query: 61  NK-RSREIED---FSRQQKLQISLNYAC-QDDADRSTSIRNPNAVSTGLRLSYDDDEHNS 120
           N  R RE E+     RQQKLQISLNY    ++      +   N VSTGLRLSYDDDE NS
Sbjct: 61  NSTRGRETENNNIMQRQQKLQISLNYNYNNNNTVVQDEVPKQNLVSTGLRLSYDDDERNS 120

Query: 121 SVTTASGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKLQEEHLAKGIRDMKQRHMASF 180
           SVT+A+GSIT    +  SLGDNIR +++RQ +E DQ+IK + + +AKG+RD+KQRH+ SF
Sbjct: 121 SVTSANGSITT--PVYQSLGDNIRLDLNRQNDELDQFIKFRADQMAKGVRDIKQRHVTSF 180

Query: 181 LSAIEKGIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESVVNVLKNN 240
           ++A+EK + KKL EKD EIESMN+KNRELV++IK VA EAQNWH +AKYNESVVN LK N
Sbjct: 181 VTALEKDVSKKLQEKDHEIESMNKKNRELVDKIKQVAVEAQNWHYKAKYNESVVNALKVN 240

Query: 241 LQHAISQGAD------------QAKEGFGDSEVDDAASNIDPNNYPRVPGGTIKPSTSME 300
           LQ  +S G D            Q KEGFGDSE+DD A++    NY  +PG    PST M 
Sbjct: 241 LQQVMSHGNDNNAVGGGVADHHQMKEGFGDSEIDDEAASY---NYLNIPG---MPSTGMR 300

Query: 301 HMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCHLIKTAGIQVY 332
              C+ C  K VS+LL+PCRHL LCKDCD    VCPVC  +KT+ +QV+
Sbjct: 301 ---CKLCNVKNVSVLLVPCRHLSLCKDCDVFTGVCPVCQSLKTSSVQVF 338

BLAST of PI0002705.1 vs. TAIR 10
Match: AT1G10650.2 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 312.8 bits (800), Expect = 3.4e-85
Identity = 172/290 (59.31%), Postives = 209/290 (72.07%), Query Frame = 0

Query: 56  MLRPNKRSREIEDFS-----RQQKLQISLNYACQDDADRSTSIRNPNAVSTGLRLSYDDD 115
           M+RPNKR RE E  S     +QQKLQ+SLNY   + + R   +   N VSTGLRLSYDDD
Sbjct: 1   MIRPNKRGREAESISNNIQRQQQKLQMSLNYNYNNTSVRE-EVPKENLVSTGLRLSYDDD 60

Query: 116 EHNSSVTTASGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKLQEEHLAKGIRDMKQRH 175
           EHNSSVT+ASGSI AA  I  SL D++R ++ RQK+EFDQ+IK+Q   +AKG+RDMKQRH
Sbjct: 61  EHNSSVTSASGSILAASPIFQSLDDSLRIDLHRQKDEFDQFIKIQAAQMAKGVRDMKQRH 120

Query: 176 MASFLSAIEKGIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESVVNV 235
           +ASFL+ +EKG+ KKL EKD EI  MN+KN+ELVERIK VA EAQNWH RAKYNESVVNV
Sbjct: 121 IASFLTTLEKGVSKKLQEKDHEINDMNKKNKELVERIKQVAMEAQNWHYRAKYNESVVNV 180

Query: 236 LKNNLQHAISQ------GADQAKEGFGDSEVDDAASN-IDPNNYPRVPGGTIKPSTSMEH 295
           LK NLQ A+S        ADQ KEGFGDSE+DDAAS+ IDPNN      G        + 
Sbjct: 181 LKANLQQAMSHNNSVIAAADQGKEGFGDSEIDDAASSYIDPNNNNNNNMGI------HQR 240

Query: 296 MICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCHLIKTAGIQVYLS 334
           M C+ C  KEVS+L++PCRHL LCK+CD    +CPVC  +K++ +QV+ S
Sbjct: 241 MRCKMCNVKEVSVLIVPCRHLSLCKECDVFTKICPVCKSLKSSCVQVFFS 283

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4IDI69.8e-2130.86Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=37... [more]
Q9LDD12.4e-1930.00Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=37... [more]
Q9FHE42.0e-1830.80BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG... [more]
O818515.9e-1830.33E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1[more]
Q134892.6e-0540.38Baculoviral IAP repeat-containing protein 3 OS=Homo sapiens OX=9606 GN=BIRC3 PE=... [more]
Match NameE-valueIdentityDescription
A0A1S3B9M99.7e-18196.70BOI-related E3 ubiquitin-protein ligase 1 OS=Cucumis melo OX=3656 GN=LOC10348769... [more]
A0A0A0LNB03.7e-18096.10RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G348840 P... [more]
A0A5A7V1718.8e-17484.29BOI-related E3 ubiquitin-protein ligase 1 OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A6J1KC061.7e-16990.86BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1HCD71.5e-16890.27BOI-related E3 ubiquitin-protein ligase 1 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
Match NameE-valueIdentityDescription
XP_008444346.12.0e-18096.70PREDICTED: BOI-related E3 ubiquitin-protein ligase 1 [Cucumis melo][more]
XP_011649486.17.6e-18096.10BOI-related E3 ubiquitin-protein ligase 1 [Cucumis sativus] >XP_011649487.1 BOI-... [more]
XP_038884634.11.1e-17895.27probable BOI-related E3 ubiquitin-protein ligase 2 [Benincasa hispida][more]
KAA0061080.11.8e-17384.29BOI-related E3 ubiquitin-protein ligase 1 [Cucumis melo var. makuwa][more]
XP_022997618.13.6e-16990.86BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita maxima] >XP_022997619.... [more]
Match NameE-valueIdentityDescription
AT1G10650.16.2e-10358.09SBP (S-ribonuclease binding protein) family protein [more]
AT1G60610.18.3e-9253.58SBP (S-ribonuclease binding protein) family protein [more]
AT1G60610.28.3e-9253.58SBP (S-ribonuclease binding protein) family protein [more]
AT1G60610.38.3e-9253.58SBP (S-ribonuclease binding protein) family protein [more]
AT1G10650.23.4e-8559.31SBP (S-ribonuclease binding protein) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 186..213
NoneNo IPR availablePFAMPF13920zf-C3HC4_3coord: 284..321
e-value: 1.1E-6
score: 28.4
NoneNo IPR availablePANTHERPTHR42647:SF9S-RIBONUCLEASE BINDING PROTEIN SBP1-RELATEDcoord: 4..332
IPR017066E3 ubiquitin-protein ligase BOI-likePIRSFPIRSF036836SBP1_RNase_bindcoord: 1..333
e-value: 8.8E-99
score: 329.3
IPR017066E3 ubiquitin-protein ligase BOI-likePANTHERPTHR42647SBP (S-RIBONUCLEASE BINDING PROTEIN) FAMILY PROTEINcoord: 4..332
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 276..333
e-value: 4.7E-8
score: 34.4
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 286..321
score: 8.830534

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
PI0002705PI0002705gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
PI0002705.1-exonPI0002705.1-exon-chr03:110415..111231exon
PI0002705.1-exonPI0002705.1-exon-chr03:111519..111877exon
PI0002705.1-exonPI0002705.1-exon-chr03:112072..112251exon
PI0002705.1-exonPI0002705.1-exon-chr03:113166..113221exon
PI0002705.1-exonPI0002705.1-exon-chr03:113354..113643exon


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
PI0002705.1-three_prime_utrPI0002705.1-three_prime_utr-chr03:110415..110694three_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
PI0002705.1-cdsPI0002705.1-cds-chr03:110695..111231CDS
PI0002705.1-cdsPI0002705.1-cds-chr03:111519..111877CDS
PI0002705.1-cdsPI0002705.1-cds-chr03:112072..112177CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
PI0002705.1-five_prime_utrPI0002705.1-five_prime_utr-chr03:112178..112251five_prime_UTR
PI0002705.1-five_prime_utrPI0002705.1-five_prime_utr-chr03:113166..113221five_prime_UTR
PI0002705.1-five_prime_utrPI0002705.1-five_prime_utr-chr03:113354..113643five_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
PI0002705.1PI0002705.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004842 ubiquitin-protein transferase activity