Cucsat.G6824.T5 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G6824.T5
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	eukaryotic translation initiation factor 4B2
Location	ctg1522: 443416 .. 445959 (-)
RNA-Seq Expression	Cucsat.G6824.T5
Synteny	Cucsat.G6824.T5

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGTGATGGACCCGGTAAGGATACCAGCACACACCGAGGGCCGATTCCCACCCTCTTATTCTTCCTTCCTCAAACATGCCTCCCTTTCTACGTTCTAAACTTTCTATACTTTCTCACTTTCTTCCTCCTTCTTCCTTTCTTCCCATCCAAACATGTCTTCAATAATCCCTGTGACTGCTTCTTCTCCACTTGGCTTCTTCCCTCAGTTTTCAGCTTCCTCCAACCTCCATTTTCAACAGTTTCGCTTTCCTCGTCTGCTCAACCGCACACTTGCGGCCTCCATATCTCATCATCTTAATCTCACTTCCTCTAACAAATTATGTAGTCCACGCCCTAACTGCGATCTCCATTCCATTGCACTTCCGGTTTATACGTCTGAATCAGTGGCCGATAAACTTCGTCGGCTTGTTTTGGAATTCGAGTCTCTCCTAGAGCCGATTGATCGGGTTAAACGACTGTTGCATTACGCGGCCATTCTGGACTCTTCGGACGAAGCCATTCGACTACCGGAGAATCGAGTGAAGGGATGCGCGGCGCAGGTGTGGTTGGATGTGAACGTTGACGAGTTCGGCCGGATGAGATTCAAAGCGGACAGTGATTCAGAGATCGCGAAAGGATATTGTTCGTGTTTGATTTGGATGCTGGAAGGTGCGGAGCCGTGGGAGGTTTTGAAGGTGAGGAGCGATGATTTGGAGGCTGTGAACGTTGGATTGCACGGAAAAGCGACATCTAGGGTTAATACATGGCAAAATGTGTTGATGAGTATGCAAATGAGGACCAACACTTTGGTTTCAAACAGTGAAATGAAACCGCCATTGGAGCCGTTTACCTCCCGTACTGTTCATCGTCGCCTCCATGGATGAAACGAAGCCTGAATTGGAGGATTTAATCTTCAGGAGCTGGTCGTTCATCATAACAAATTGAGAAAGCAACAGGGTAGATGAAATGAATCAAAATTTCATATGATCTGAAATGAGAAAAGCTTGTATTGTAATCATGCCATACCAACACTGAAACAGGTATCTTTTGATTGTAACATAAAATCATTATGTACTAATACCTCATGATATTGGATTAAGTTTTCCATTAGGTTGGAAGAGTTTTTCTGTATTAACTTCAACATCATCCATTAACCTTAAGACCCAAGTTCAGTTGTGCAACACATCCTCTCATCTATGTTGAGAATTTCACGTTTATGTTAGAAAATCTCACCTTGCAAAAACCAATTGATAACTTTAAAATGTTATCAAATAATCTATCTAACTCAAATCACACCTTTTAAATAAACTATAAATTATTAACGGGTGGCTTTTCTTCATCTTTCTATTTTTCTTGATGATAATTTCATAAAATTTTAAGACAGAATAAATTCATTTATGTTCTTTTAGTTGATTTTAAATTTTAAATGAAATGAAGTGAAAATACTAACATACTTAGGGATTAATTAACAAGTTGATATGATTGAATTAATCCTTTGATAGATGCATAATGACGTGTAAAAAACCAACTCACAAATAAAAAAGTCTTCCATTTAAAAACACAATAAAACTCTTCAAATCAACTTATCATTGGTCTTTAACATAGCTTTTTTCTTTTAAAATAACTATCTTACAACTTATTTTAAGAAATAACATAGCCTAATTTCACGAAAAGAAAGTAGCATAAACTATATATATATAACTTACGATCTGTTGATATTAGACATAAAGGTGAATATTATGAGTTTAGAAGCTAATAAGTTGGAAAGGTGATTGAAAAGAGGGAAACAAAGGAGGTCTTATCGCGGGTAGCTTTAGTCCTATAGAAAGAAACCTCTTTAATAAAAAAGAAAAAAGAGTCAAACCTAAGGTTGAAGATATAAATTGTTTTGCCTGCGATTTAGCTAATAGTGAACCACACCCTTATTTATTTCTCTTTTATGTGTTTGTGTTCTGTATTGGCTGATTTCTTAACTTTTTGTTTTTTGGTAAATAAAATTCTTACTCTTTAAAAAAAAAAGAACAATAGGTGCACTAAGTGGACAGAGTGTTGTATCTAAAAGGTCATTATTGGACTGATGAAAAGCAAAGAAGAAAGGGATTTAATAAGAAGAAAACAAAAATGGAAGACAGCAGTGAACGGAAGTTTAGTATAAATTATAATGAATTGTTATTGTTATTATTTATTACGATCGTGCAAGTAAATTGAAGTGAAGTGGATTATTATTATAAAATGGGATGAAATAAACTGATAACAGTTTCTCGAAAAGATAAAAAGAAAGCTGACAGATTATTCACAGGTAATAAAGAAATTTATAGGTAAAGAAACTTGTTTATGTATGTAATGAGTAAATAGTGTATGTAACCAAAGCATGTAAGAAGCAAGTATTTAAGTGTATGAACGAAACCTCAAAACTAATGAAGGTTATAGAAATTACCAGAAAAAGGACAATAATGTTTATACAAAATTGATAGATTAACGAAAAGTAATTGAGAAATTGATAGATTGATTTGATTTTGTAGAAGGGATTGGGTTGGTTTAGAAGTTAGAAGTACACCCGCACCC

Coding sequence (CDS)

ATGTCTTCAATAATCCCTGTGACTGCTTCTTCTCCACTTGGCTTCTTCCCTCAGTTTTCAGCTTCCTCCAACCTCCATTTTCAACAGTTTCGCTTTCCTCGTCTGCTCAACCGCACACTTGCGGCCTCCATATCTCATCATCTTAATCTCACTTCCTCTAACAAATTATGTAGTCCACGCCCTAACTGCGATCTCCATTCCATTGCACTTCCGGTTTATACGTCTGAATCAGTGGCCGATAAACTTCGTCGGCTTGTTTTGGAATTCGAGTCTCTCCTAGAGCCGATTGATCGGGTTAAACGACTGTTGCATTACGCGGCCATTCTGGACTCTTCGGACGAAGCCATTCGACTACCGGAGAATCGAGTGAAGGGATGCGCGGCGCAGGTGTGGTTGGATGTGAACGTTGACGAGTTCGGCCGGATGAGATTCAAAGCGGACAGTGATTCAGAGATCGCGAAAGGATATTGTTCGTGTTTGATTTGGATGCTGGAAGGTGCGGAGCCGTGGGAGGTTTTGAAGGTGAGGAGCGATGATTTGGAGGCTGTGAACGTTGGATTGCACGGAAAAGCGACATCTAGGGTTAATACATGGCAAAATGTGTTGATGAGTATGCAAATGAGGACCAACACTTTGGTTTCAAACAGTGAAATGAAACCGCCATTGGAGCCGTTTACCTCCCGTACTGTTCATCGTCGCCTCCATGGATGA

Protein sequence

MSSIIPVTASSPLGFFPQFSASSNLHFQQFRFPRLLNRTLAASISHHLNLTSSNKLCSPRPNCDLHSIALPVYTSESVADKLRRLVLEFESLLEPIDRVKRLLHYAAILDSSDEAIRLPENRVKGCAAQVWLDVNVDEFGRMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVLKVRSDDLEAVNVGLHGKATSRVNTWQNVLMSMQMRTNTLVSNSEMKPPLEPFTSRTVHRRLHG

Homology

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BLAST of Cucsat.G6824.T5 vs. ExPASy Swiss-Prot
Match: Q9FXE3 (SufE-like protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SUFE2 PE=2 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 9.9e-52
Identity = 111/201 (55.22%), Postives = 138/201 (68.66%), Query Frame = 0


Query: 17  PQFSASSNLHFQQFRFPRLLNRTLAASIS---HHLNLTSSNKLCSPRPNCDLHSIALPVY 76
           P   ++S    + F  PR  +R     I+     LNL S+ K  SP     L ++++   
Sbjct: 13  PPLISTSRPTTKSFPNPRFTSRFSPKPITCMRDSLNLGSNPKAPSP---FSLATVSVDAP 72

Query: 77  TSESVADKLRRLVLEFESLLEPIDRVKRLLHYAAILDSSDEAIRLPENRVKGCAAQVWLD 136
                +DKLR LV EF SL EPIDRVKRLL+YAA L   DE+ R+ ENRV GC  QVWL+
Sbjct: 73  LGTKTSDKLRILVSEFRSLTEPIDRVKRLLNYAATLAPLDESARISENRVTGCTTQVWLE 132

Query: 137 VNVDEFGRMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVLKVRSDDLEAVNVGLHGKATS 196
           + +DEFGRMRFKADSDSEI+KG+CSCLIW+L+GA+P EV+ VRS+DL  +NVG+HGK  S
Sbjct: 133 IKMDEFGRMRFKADSDSEISKGFCSCLIWILDGAKPEEVMGVRSEDLSEMNVGVHGKEQS 192

Query: 197 RVNTWQNVLMSMQMRTNTLVS 215
           RVNTW NVLMSMQ RT TLV+
Sbjct: 193 RVNTWHNVLMSMQKRTMTLVA 210

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BLAST of Cucsat.G6824.T5 vs. ExPASy Swiss-Prot
Match: Q9FGS4 (Quinolinate synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=QS PE=1 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 5.1e-40
Identity = 84/155 (54.19%), Postives = 112/155 (72.26%), Query Frame = 0


Query: 74  TSESVADKLRRLVLEFESLLEPIDRVKRLLHYAAILDSSDEAIRLPENRVKGCAAQVWLD 133
           T+E V  KL+RLV EF+SL EPIDR+K +LHYA++L    E+ +   NRV GC A+VWLD
Sbjct: 80  TTELVPYKLQRLVKEFKSLTEPIDRLKWVLHYASLLPQMPESSKTESNRVMGCTARVWLD 139

Query: 134 VNVDEFGRMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVLKVRSDDLEAVNVGLHGKATS 193
             + + G+MRF ADSDS+++KG CSCLI +L+ A P EV++++++DL  +NVGL G   S
Sbjct: 140 AELGQDGKMRFCADSDSDVSKGMCSCLIQVLDEASPVEVMELKTEDLAELNVGLLGGERS 199

Query: 194 RVNTWQNVLMSMQMRTNTLVSNSEMK-PPLEPFTS 228
           RVNTW NVL+SMQ +T  LV+  E K P  EPF S
Sbjct: 200 RVNTWYNVLVSMQKKTRRLVAEREGKVPSFEPFPS 234

[Close]

BLAST of Cucsat.G6824.T5 vs. ExPASy Swiss-Prot
Match: Q2QTL0 (Quinolinate synthase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0290150 PE=3 SV=2)

HSP 1 Score: 125.6 bits (314), Expect = 7.7e-28
Identity = 71/153 (46.41%), Postives = 89/153 (58.17%), Query Frame = 0


Query: 81  KLRRLVLEFESLLEPIDRVKRLLHYAAILDSSDEAIRLPENRVKGCAAQVWLDVNVDEFG 140
           ++ RL  EF    +  DR +RLL  AA L    EA R+  NRV GC AQVWL    D  G
Sbjct: 69  RVSRLAEEFRVSPDAADRARRLLARAAALPRLGEADRVAANRVMGCVAQVWLVGRCDGAG 128

Query: 141 RMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVLKVRSDDLEAVNVGLHGK-ATSRVNTWQ 200
           RMRF ADSDSE+++GYC+CL+  L+GA P EVL V   DL  +     G  A SR +TW 
Sbjct: 129 RMRFAADSDSELSRGYCACLVSALDGARPEEVLDVDPADLAPLGGAAAGTGARSRASTWH 188

Query: 201 NVLMSMQMRTNTLVSNSEMKPPLEPFTSRTVHR 233
           NVL+ MQ R    ++  E +P  EPF S  + R
Sbjct: 189 NVLIGMQKRARAAIAAREGRPAGEPFPSLIIGR 221

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BLAST of Cucsat.G6824.T5 vs. ExPASy Swiss-Prot
Match: Q84W65 (SufE-like protein 1, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SUFE1 PE=1 SV=2)

HSP 1 Score: 73.6 bits (179), Expect = 3.5e-12
Identity = 46/134 (34.33%), Postives = 73/134 (54.48%), Query Frame = 0


Query: 76  ESVADKLRRLVLEFESLLEPIDRVKRLLHYAAILDSSDEAIRLPENRVKGCAAQVWLDVN 135
           E +  KL+ +V  F+S+ EP  + ++L+ Y   L   D   +  EN+V+GC +QVW+   
Sbjct: 81  EELPPKLQEIVKLFQSVQEPKAKYEQLMFYGKNLTPLDSQFKTRENKVEGCVSQVWVRAF 140

Query: 136 VDEFGRMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVLKVRSDDLEAVNVGL-HGKATSR 195
            DE   + ++ADSDS + KG  + L+  L G    E+L++  D   AV +GL    + SR
Sbjct: 141 FDEERNVVYEADSDSVLTKGLAALLVKGLSGRPVPEILRITPD--FAVLLGLQQSLSPSR 200

Query: 196 VNTWQNVLMSMQMR 209
            N   N+L  MQ +
Sbjct: 201 NNGLLNMLKLMQKK 212

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BLAST of Cucsat.G6824.T5 vs. ExPASy Swiss-Prot
Match: Q52967 (Uncharacterized SufE-like protein R01000 OS=Rhizobium meliloti (strain 1021) OX=266834 GN=R01000 PE=3 SV=2)

HSP 1 Score: 50.8 bits (120), Expect = 2.4e-05
Identity = 28/102 (27.45%), Postives = 53/102 (51.96%), Query Frame = 0


Query: 82  LRRLVLEFESLLEPIDRVKRLLHYAAILDSSDEAIRLPENRVKGCAAQVWLDVNVD---E 141
           L +++ +F  L E  DR + ++     L    E  R  EN+V+GCA+QVWL  +     E
Sbjct: 4   LDQIIDDFAFLDEWEDRYRYVIELGKNLPEMPEVSRTSENKVQGCASQVWLVTHATGDAE 63

Query: 142 FGRMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVLKVRSDDL 181
              + F+ +SD+ I +G  + ++ +  G    E+ ++ + D+
Sbjct: 64  DPLLTFEGESDAHIVRGLVAIVLAIFSGKRASEITRIDALDI 105

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BLAST of Cucsat.G6824.T5 vs. NCBI nr
Match: XP_004143058.1 (sufE-like protein 2, chloroplastic [Cucumis sativus] >KGN62329.1 hypothetical protein Csa_018584 [Cucumis sativus])

HSP 1 Score: 470 bits (1210), Expect = 3.65e-167
Identity = 236/236 (100.00%), Postives = 236/236 (100.00%), Query Frame = 0


Query: 1   MSSIIPVTASSPLGFFPQFSASSNLHFQQFRFPRLLNRTLAASISHHLNLTSSNKLCSPR 60
           MSSIIPVTASSPLGFFPQFSASSNLHFQQFRFPRLLNRTLAASISHHLNLTSSNKLCSPR
Sbjct: 1   MSSIIPVTASSPLGFFPQFSASSNLHFQQFRFPRLLNRTLAASISHHLNLTSSNKLCSPR 60

Query: 61  PNCDLHSIALPVYTSESVADKLRRLVLEFESLLEPIDRVKRLLHYAAILDSSDEAIRLPE 120
           PNCDLHSIALPVYTSESVADKLRRLVLEFESLLEPIDRVKRLLHYAAILDSSDEAIRLPE
Sbjct: 61  PNCDLHSIALPVYTSESVADKLRRLVLEFESLLEPIDRVKRLLHYAAILDSSDEAIRLPE 120

Query: 121 NRVKGCAAQVWLDVNVDEFGRMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVLKVRSDDL 180
           NRVKGCAAQVWLDVNVDEFGRMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVLKVRSDDL
Sbjct: 121 NRVKGCAAQVWLDVNVDEFGRMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVLKVRSDDL 180

Query: 181 EAVNVGLHGKATSRVNTWQNVLMSMQMRTNTLVSNSEMKPPLEPFTSRTVHRRLHG 236
           EAVNVGLHGKATSRVNTWQNVLMSMQMRTNTLVSNSEMKPPLEPFTSRTVHRRLHG
Sbjct: 181 EAVNVGLHGKATSRVNTWQNVLMSMQMRTNTLVSNSEMKPPLEPFTSRTVHRRLHG 236

[Close]

BLAST of Cucsat.G6824.T5 vs. NCBI nr
Match: KAA0061053.1 (sufE-like protein 2 [Cucumis melo var. makuwa])

HSP 1 Score: 385 bits (990), Expect = 1.43e-133
Identity = 199/240 (82.92%), Postives = 211/240 (87.92%), Query Frame = 0


Query: 3   SIIPVTASSPLGFFPQFSASSNLHFQQFRFPRLLNRTLAAS------ISHHLNLTSSNKL 62
           S IP+ ASS + F   FS SS LHFQQFR P ++NR  AAS      ISH LNLTSSNKL
Sbjct: 2   SSIPMIASSRIAF-SDFSGSSKLHFQQFRSPHVVNRRFAASKSVRCAISHDLNLTSSNKL 61

Query: 63  CSPRPNCDLHSIALPVYTSESVADKLRRLVLEFESLLEPIDRVKRLLHYAAILDSSDEAI 122
           CSPRPNCDL SIA+PVY+SE VADKLRRLVLEFESLLEPIDRVKRLLHYAAIL SSD+AI
Sbjct: 62  CSPRPNCDLDSIAIPVYSSELVADKLRRLVLEFESLLEPIDRVKRLLHYAAILGSSDKAI 121

Query: 123 RLPENRVKGCAAQVWLDVNVDEFGRMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVLKVR 182
           R+PENRV+GCAAQVWLDV +DEF RMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVL VR
Sbjct: 122 RIPENRVRGCAAQVWLDVKIDEFDRMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVLNVR 181

Query: 183 SDDLEAVNVGLHGKATSRVNTWQNVLMSMQMRTNTLVSNSEMKPPLEPFTSRTVHRRLHG 236
           SDDLEAVNVGLHGKATSRVNTWQNVLMSMQMRTNTLVS +E+KP LEPFT RTVHRRLHG
Sbjct: 182 SDDLEAVNVGLHGKATSRVNTWQNVLMSMQMRTNTLVSKTEVKPLLEPFTFRTVHRRLHG 240

[Close]

BLAST of Cucsat.G6824.T5 vs. NCBI nr
Match: XP_008444379.1 (PREDICTED: sufE-like protein 2, chloroplastic [Cucumis melo])

HSP 1 Score: 377 bits (968), Expect = 2.59e-130
Identity = 197/240 (82.08%), Postives = 207/240 (86.25%), Query Frame = 0


Query: 3   SIIPVTASSPLGFFPQFSASSNLHFQQFRFPRLLNRTLAAS------ISHHLNLTSSNKL 62
           S IP+ ASS + F   FS SS LHFQQFR P ++NR  AAS      ISH LNLTSSNKL
Sbjct: 2   SSIPMIASSRIAF-SDFSGSSKLHFQQFRSPHVVNRRFAASKSVRCAISHDLNLTSSNKL 61

Query: 63  CSPRPNCDLHSIALPVYTSESVADKLRRLVLEFESLLEPIDRVKRLLHYAAILDSSDEAI 122
           CSPRPNCDL SIA+PVY+SE VADKLRRLVLEFESLLEPIDRVKRLLHYAAIL SSD+AI
Sbjct: 62  CSPRPNCDLDSIAIPVYSSELVADKLRRLVLEFESLLEPIDRVKRLLHYAAILGSSDKAI 121

Query: 123 RLPENRVKGCAAQVWLDVNVDEFGRMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVLKVR 182
           R+PENRV+GCAAQVWLDV +DEF RMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVL VR
Sbjct: 122 RIPENRVRGCAAQVWLDVKIDEFDRMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVLNVR 181

Query: 183 SDDLEAVNVGLHGKATSRVNTWQNVLMSMQMRTNTLVSNSEMKPPLEPFTSRTVHRRLHG 236
           SDDLEAVNVGLHGKATSRVNTWQNVLMSMQMRTNTL      KPPLEPFT RTVHRRLHG
Sbjct: 182 SDDLEAVNVGLHGKATSRVNTWQNVLMSMQMRTNTL------KPPLEPFTFRTVHRRLHG 234

[Close]

BLAST of Cucsat.G6824.T5 vs. NCBI nr
Match: XP_038886328.1 (sufE-like protein 2, chloroplastic [Benincasa hispida])

HSP 1 Score: 368 bits (945), Expect = 2.73e-126
Identity = 191/240 (79.58%), Postives = 202/240 (84.17%), Query Frame = 0


Query: 3   SIIPVTASSPLGFFPQFSASSNLHFQQFRFPRLLNRTLAAS------ISHHLNLTSSNKL 62
           S IP+  SS +GF  +FS SSNL  QQ   P +LNR   AS      ISH LNLTSSNKL
Sbjct: 30  SSIPMITSSGIGF-AKFSGSSNLCLQQIPSPHMLNRRFVASKSVTCTISHDLNLTSSNKL 89

Query: 63  CSPRPNCDLHSIALPVYTSESVADKLRRLVLEFESLLEPIDRVKRLLHYAAILDSSDEAI 122
           C+ RPNCDL SIA+P+YTSE VAD+L RLVLEFESLLEPIDRVKRLLHYAAILD SDE+I
Sbjct: 90  CTRRPNCDLDSIAIPLYTSELVADRLHRLVLEFESLLEPIDRVKRLLHYAAILDPSDESI 149

Query: 123 RLPENRVKGCAAQVWLDVNVDEFGRMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVLKVR 182
           RLPENRV+GCA QVWLDV +DEF RMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVLKVR
Sbjct: 150 RLPENRVRGCATQVWLDVKIDEFDRMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVLKVR 209

Query: 183 SDDLEAVNVGLHGKATSRVNTWQNVLMSMQMRTNTLVSNSEMKPPLEPFTSRTVHRRLHG 236
           SDDLEAVNVGLHGKA SRVNTWQNVLMSMQMRT TLVS  E KPPLEPFT RTVH RLHG
Sbjct: 210 SDDLEAVNVGLHGKAASRVNTWQNVLMSMQMRTKTLVSKIEAKPPLEPFTFRTVHHRLHG 268

[Close]

BLAST of Cucsat.G6824.T5 vs. NCBI nr
Match: XP_022131476.1 (sufE-like protein 2, chloroplastic [Momordica charantia])

HSP 1 Score: 332 bits (850), Expect = 2.57e-112
Identity = 176/237 (74.26%), Postives = 191/237 (80.59%), Query Frame = 0


Query: 3   SIIPVTASSPLGFFPQFSASSNLHFQQFRFPRLLNRTLAAS------ISHHLNLTSSNKL 62
           S IP+  S   GF  +FS SS L F++ RF ++ N    AS      ISH LN+TSSNKL
Sbjct: 2   SSIPIITSCGFGF-AEFSDSSKLSFERIRFRQVENTRFLASKSVRCAISHDLNVTSSNKL 61

Query: 63  CSPRPNCDLHSIALPVYTSESVADKLRRLVLEFESLLEPIDRVKRLLHYAAILDSSDEAI 122
           CS RP CDL +I +PVY SE VADK+ RLVLEFESLLEPIDRVKRLLHYAAILD SDE+I
Sbjct: 62  CSRRPKCDLDAITIPVYASELVADKIHRLVLEFESLLEPIDRVKRLLHYAAILDPSDESI 121

Query: 123 RLPENRVKGCAAQVWLDVNVDEFGRMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVLKVR 182
           RLPENRVKGC  QVWLDV +DEF RMRFKADSDSEIAKGYCSCLIWML+GAEP EVLKVR
Sbjct: 122 RLPENRVKGCXTQVWLDVKIDEFERMRFKADSDSEIAKGYCSCLIWMLDGAEPLEVLKVR 181

Query: 183 SDDLEAVNVGLHGKATSRVNTWQNVLMSMQMRTNTLVSNSEMKPPLEPFTSRTVHRR 233
           SDDL+AVNVGLHGKA SRVNTWQNV+MSMQMRTN LVS SE KPPLEPF   TVH R
Sbjct: 182 SDDLDAVNVGLHGKAISRVNTWQNVMMSMQMRTNALVSRSEPKPPLEPFII-TVHHR 236

[Close]

BLAST of Cucsat.G6824.T5 vs. ExPASy TrEMBL
Match: A0A0A0LMT7 (SufE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G349630 PE=4 SV=1)

HSP 1 Score: 470 bits (1210), Expect = 1.77e-167
Identity = 236/236 (100.00%), Postives = 236/236 (100.00%), Query Frame = 0


Query: 1   MSSIIPVTASSPLGFFPQFSASSNLHFQQFRFPRLLNRTLAASISHHLNLTSSNKLCSPR 60
           MSSIIPVTASSPLGFFPQFSASSNLHFQQFRFPRLLNRTLAASISHHLNLTSSNKLCSPR
Sbjct: 1   MSSIIPVTASSPLGFFPQFSASSNLHFQQFRFPRLLNRTLAASISHHLNLTSSNKLCSPR 60

Query: 61  PNCDLHSIALPVYTSESVADKLRRLVLEFESLLEPIDRVKRLLHYAAILDSSDEAIRLPE 120
           PNCDLHSIALPVYTSESVADKLRRLVLEFESLLEPIDRVKRLLHYAAILDSSDEAIRLPE
Sbjct: 61  PNCDLHSIALPVYTSESVADKLRRLVLEFESLLEPIDRVKRLLHYAAILDSSDEAIRLPE 120

Query: 121 NRVKGCAAQVWLDVNVDEFGRMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVLKVRSDDL 180
           NRVKGCAAQVWLDVNVDEFGRMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVLKVRSDDL
Sbjct: 121 NRVKGCAAQVWLDVNVDEFGRMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVLKVRSDDL 180

Query: 181 EAVNVGLHGKATSRVNTWQNVLMSMQMRTNTLVSNSEMKPPLEPFTSRTVHRRLHG 236
           EAVNVGLHGKATSRVNTWQNVLMSMQMRTNTLVSNSEMKPPLEPFTSRTVHRRLHG
Sbjct: 181 EAVNVGLHGKATSRVNTWQNVLMSMQMRTNTLVSNSEMKPPLEPFTSRTVHRRLHG 236

[Close]

BLAST of Cucsat.G6824.T5 vs. ExPASy TrEMBL
Match: A0A5A7UYK2 (SufE-like protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G001910 PE=4 SV=1)

HSP 1 Score: 385 bits (990), Expect = 6.91e-134
Identity = 199/240 (82.92%), Postives = 211/240 (87.92%), Query Frame = 0


Query: 3   SIIPVTASSPLGFFPQFSASSNLHFQQFRFPRLLNRTLAAS------ISHHLNLTSSNKL 62
           S IP+ ASS + F   FS SS LHFQQFR P ++NR  AAS      ISH LNLTSSNKL
Sbjct: 2   SSIPMIASSRIAF-SDFSGSSKLHFQQFRSPHVVNRRFAASKSVRCAISHDLNLTSSNKL 61

Query: 63  CSPRPNCDLHSIALPVYTSESVADKLRRLVLEFESLLEPIDRVKRLLHYAAILDSSDEAI 122
           CSPRPNCDL SIA+PVY+SE VADKLRRLVLEFESLLEPIDRVKRLLHYAAIL SSD+AI
Sbjct: 62  CSPRPNCDLDSIAIPVYSSELVADKLRRLVLEFESLLEPIDRVKRLLHYAAILGSSDKAI 121

Query: 123 RLPENRVKGCAAQVWLDVNVDEFGRMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVLKVR 182
           R+PENRV+GCAAQVWLDV +DEF RMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVL VR
Sbjct: 122 RIPENRVRGCAAQVWLDVKIDEFDRMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVLNVR 181

Query: 183 SDDLEAVNVGLHGKATSRVNTWQNVLMSMQMRTNTLVSNSEMKPPLEPFTSRTVHRRLHG 236
           SDDLEAVNVGLHGKATSRVNTWQNVLMSMQMRTNTLVS +E+KP LEPFT RTVHRRLHG
Sbjct: 182 SDDLEAVNVGLHGKATSRVNTWQNVLMSMQMRTNTLVSKTEVKPLLEPFTFRTVHRRLHG 240

[Close]

BLAST of Cucsat.G6824.T5 vs. ExPASy TrEMBL
Match: A0A1S3BAY5 (sufE-like protein 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487725 PE=4 SV=1)

HSP 1 Score: 377 bits (968), Expect = 1.25e-130
Identity = 197/240 (82.08%), Postives = 207/240 (86.25%), Query Frame = 0


Query: 3   SIIPVTASSPLGFFPQFSASSNLHFQQFRFPRLLNRTLAAS------ISHHLNLTSSNKL 62
           S IP+ ASS + F   FS SS LHFQQFR P ++NR  AAS      ISH LNLTSSNKL
Sbjct: 2   SSIPMIASSRIAF-SDFSGSSKLHFQQFRSPHVVNRRFAASKSVRCAISHDLNLTSSNKL 61

Query: 63  CSPRPNCDLHSIALPVYTSESVADKLRRLVLEFESLLEPIDRVKRLLHYAAILDSSDEAI 122
           CSPRPNCDL SIA+PVY+SE VADKLRRLVLEFESLLEPIDRVKRLLHYAAIL SSD+AI
Sbjct: 62  CSPRPNCDLDSIAIPVYSSELVADKLRRLVLEFESLLEPIDRVKRLLHYAAILGSSDKAI 121

Query: 123 RLPENRVKGCAAQVWLDVNVDEFGRMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVLKVR 182
           R+PENRV+GCAAQVWLDV +DEF RMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVL VR
Sbjct: 122 RIPENRVRGCAAQVWLDVKIDEFDRMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVLNVR 181

Query: 183 SDDLEAVNVGLHGKATSRVNTWQNVLMSMQMRTNTLVSNSEMKPPLEPFTSRTVHRRLHG 236
           SDDLEAVNVGLHGKATSRVNTWQNVLMSMQMRTNTL      KPPLEPFT RTVHRRLHG
Sbjct: 182 SDDLEAVNVGLHGKATSRVNTWQNVLMSMQMRTNTL------KPPLEPFTFRTVHRRLHG 234

[Close]

BLAST of Cucsat.G6824.T5 vs. ExPASy TrEMBL
Match: A0A6J1BPL7 (sufE-like protein 2, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111004673 PE=4 SV=1)

HSP 1 Score: 332 bits (850), Expect = 1.24e-112
Identity = 176/237 (74.26%), Postives = 191/237 (80.59%), Query Frame = 0


Query: 3   SIIPVTASSPLGFFPQFSASSNLHFQQFRFPRLLNRTLAAS------ISHHLNLTSSNKL 62
           S IP+  S   GF  +FS SS L F++ RF ++ N    AS      ISH LN+TSSNKL
Sbjct: 2   SSIPIITSCGFGF-AEFSDSSKLSFERIRFRQVENTRFLASKSVRCAISHDLNVTSSNKL 61

Query: 63  CSPRPNCDLHSIALPVYTSESVADKLRRLVLEFESLLEPIDRVKRLLHYAAILDSSDEAI 122
           CS RP CDL +I +PVY SE VADK+ RLVLEFESLLEPIDRVKRLLHYAAILD SDE+I
Sbjct: 62  CSRRPKCDLDAITIPVYASELVADKIHRLVLEFESLLEPIDRVKRLLHYAAILDPSDESI 121

Query: 123 RLPENRVKGCAAQVWLDVNVDEFGRMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVLKVR 182
           RLPENRVKGC  QVWLDV +DEF RMRFKADSDSEIAKGYCSCLIWML+GAEP EVLKVR
Sbjct: 122 RLPENRVKGCXTQVWLDVKIDEFERMRFKADSDSEIAKGYCSCLIWMLDGAEPLEVLKVR 181

Query: 183 SDDLEAVNVGLHGKATSRVNTWQNVLMSMQMRTNTLVSNSEMKPPLEPFTSRTVHRR 233
           SDDL+AVNVGLHGKA SRVNTWQNV+MSMQMRTN LVS SE KPPLEPF   TVH R
Sbjct: 182 SDDLDAVNVGLHGKAISRVNTWQNVMMSMQMRTNALVSRSEPKPPLEPFII-TVHHR 236

[Close]

BLAST of Cucsat.G6824.T5 vs. ExPASy TrEMBL
Match: A0A6J1KU63 (sufE-like protein 2, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111496439 PE=4 SV=1)

HSP 1 Score: 280 bits (715), Expect = 2.29e-92
Identity = 143/200 (71.50%), Postives = 163/200 (81.50%), Query Frame = 0


Query: 18  QFSASSNLHFQQFRFPRLLNRTLAASISHHLNLTSSNKLCSPRPNCDLHSIALPVYTSES 77
           +FS SS LH Q+ R   ++N+   AS S    ++SS++      NCDL + A+PVYTS+ 
Sbjct: 15  EFSGSSKLHLQKMRSRYVVNQRFLASKSLRRTISSSDRFHLRHSNCDLDAFAIPVYTSDL 74

Query: 78  VADKLRRLVLEFESLLEPIDRVKRLLHYAAILDSSDEAIRLPENRVKGCAAQVWLDVNVD 137
           V DKL RLVLEF+ LL+PIDRVKRLL YAAILD S E+IRLPENRV+GCA QVWLDV +D
Sbjct: 75  VVDKLHRLVLEFKPLLKPIDRVKRLLQYAAILDPSGESIRLPENRVRGCATQVWLDVRID 134

Query: 138 EFGRMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVLKVRSDDLEAVNVGLHGKATSRVNT 197
           EF RMRFKADSDSEIAKGYCSCLIWMLEGA+P EVLK+RSDDLEAVNVGLHGKA SRVNT
Sbjct: 135 EFDRMRFKADSDSEIAKGYCSCLIWMLEGAKPCEVLKMRSDDLEAVNVGLHGKAISRVNT 194

Query: 198 WQNVLMSMQMRTNTLVSNSE 217
           WQNVLMSMQMRTN LVS +E
Sbjct: 195 WQNVLMSMQMRTNALVSRNE 214

[Close]

BLAST of Cucsat.G6824.T5 vs. TAIR 10
Match: AT1G67810.1 (sulfur E2 )

HSP 1 Score: 204.9 bits (520), Expect = 7.1e-53
Identity = 111/201 (55.22%), Postives = 138/201 (68.66%), Query Frame = 0


Query: 17  PQFSASSNLHFQQFRFPRLLNRTLAASIS---HHLNLTSSNKLCSPRPNCDLHSIALPVY 76
           P   ++S    + F  PR  +R     I+     LNL S+ K  SP     L ++++   
Sbjct: 13  PPLISTSRPTTKSFPNPRFTSRFSPKPITCMRDSLNLGSNPKAPSP---FSLATVSVDAP 72

Query: 77  TSESVADKLRRLVLEFESLLEPIDRVKRLLHYAAILDSSDEAIRLPENRVKGCAAQVWLD 136
                +DKLR LV EF SL EPIDRVKRLL+YAA L   DE+ R+ ENRV GC  QVWL+
Sbjct: 73  LGTKTSDKLRILVSEFRSLTEPIDRVKRLLNYAATLAPLDESARISENRVTGCTTQVWLE 132

Query: 137 VNVDEFGRMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVLKVRSDDLEAVNVGLHGKATS 196
           + +DEFGRMRFKADSDSEI+KG+CSCLIW+L+GA+P EV+ VRS+DL  +NVG+HGK  S
Sbjct: 133 IKMDEFGRMRFKADSDSEISKGFCSCLIWILDGAKPEEVMGVRSEDLSEMNVGVHGKEQS 192

Query: 197 RVNTWQNVLMSMQMRTNTLVS 215
           RVNTW NVLMSMQ RT TLV+
Sbjct: 193 RVNTWHNVLMSMQKRTMTLVA 210

[Close]

BLAST of Cucsat.G6824.T5 vs. TAIR 10
Match: AT5G50210.1 (quinolinate synthase )

HSP 1 Score: 166.0 bits (419), Expect = 3.6e-41
Identity = 84/155 (54.19%), Postives = 112/155 (72.26%), Query Frame = 0


Query: 74  TSESVADKLRRLVLEFESLLEPIDRVKRLLHYAAILDSSDEAIRLPENRVKGCAAQVWLD 133
           T+E V  KL+RLV EF+SL EPIDR+K +LHYA++L    E+ +   NRV GC A+VWLD
Sbjct: 80  TTELVPYKLQRLVKEFKSLTEPIDRLKWVLHYASLLPQMPESSKTESNRVMGCTARVWLD 139

Query: 134 VNVDEFGRMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVLKVRSDDLEAVNVGLHGKATS 193
             + + G+MRF ADSDS+++KG CSCLI +L+ A P EV++++++DL  +NVGL G   S
Sbjct: 140 AELGQDGKMRFCADSDSDVSKGMCSCLIQVLDEASPVEVMELKTEDLAELNVGLLGGERS 199

Query: 194 RVNTWQNVLMSMQMRTNTLVSNSEMK-PPLEPFTS 228
           RVNTW NVL+SMQ +T  LV+  E K P  EPF S
Sbjct: 200 RVNTWYNVLVSMQKKTRRLVAEREGKVPSFEPFPS 234

[Close]

BLAST of Cucsat.G6824.T5 vs. TAIR 10
Match: AT4G26500.1 (chloroplast sulfur E )

HSP 1 Score: 73.6 bits (179), Expect = 2.5e-13
Identity = 46/134 (34.33%), Postives = 73/134 (54.48%), Query Frame = 0


Query: 76  ESVADKLRRLVLEFESLLEPIDRVKRLLHYAAILDSSDEAIRLPENRVKGCAAQVWLDVN 135
           E +  KL+ +V  F+S+ EP  + ++L+ Y   L   D   +  EN+V+GC +QVW+   
Sbjct: 81  EELPPKLQEIVKLFQSVQEPKAKYEQLMFYGKNLTPLDSQFKTRENKVEGCVSQVWVRAF 140

Query: 136 VDEFGRMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVLKVRSDDLEAVNVGL-HGKATSR 195
            DE   + ++ADSDS + KG  + L+  L G    E+L++  D   AV +GL    + SR
Sbjct: 141 FDEERNVVYEADSDSVLTKGLAALLVKGLSGRPVPEILRITPD--FAVLLGLQQSLSPSR 200

Query: 196 VNTWQNVLMSMQMR 209
            N   N+L  MQ +
Sbjct: 201 NNGLLNMLKLMQKK 212

The following BLAST results are available for this feature:

BLAST of Cucsat.G6824.T5 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FXE3	9.9e-52	55.22	SufE-like protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SUFE2 PE=2...	[more]
Q9FGS4	5.1e-40	54.19	Quinolinate synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=QS PE=1 S...	[more]
Q2QTL0	7.7e-28	46.41	Quinolinate synthase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=...	[more]
Q84W65	3.5e-12	34.33	SufE-like protein 1, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702...	[more]
Q52967	2.4e-05	27.45	Uncharacterized SufE-like protein R01000 OS=Rhizobium meliloti (strain 1021) OX=...	[more]

BLAST of Cucsat.G6824.T5 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004143058.1	3.65e-167	100.00	sufE-like protein 2, chloroplastic [Cucumis sativus] >KGN62329.1 hypothetical pr...	[more]
KAA0061053.1	1.43e-133	82.92	sufE-like protein 2 [Cucumis melo var. makuwa]	[more]
XP_008444379.1	2.59e-130	82.08	PREDICTED: sufE-like protein 2, chloroplastic [Cucumis melo]	[more]
XP_038886328.1	2.73e-126	79.58	sufE-like protein 2, chloroplastic [Benincasa hispida]	[more]
XP_022131476.1	2.57e-112	74.26	sufE-like protein 2, chloroplastic [Momordica charantia]	[more]

BLAST of Cucsat.G6824.T5 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LMT7	1.77e-167	100.00	SufE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G349630 PE=4 S...	[more]
A0A5A7UYK2	6.91e-134	82.92	SufE-like protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G...	[more]
A0A1S3BAY5	1.25e-130	82.08	sufE-like protein 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487725 PE=4 ...	[more]
A0A6J1BPL7	1.24e-112	74.26	sufE-like protein 2, chloroplastic OS=Momordica charantia OX=3673 GN=LOC11100467...	[more]
A0A6J1KU63	2.29e-92	71.50	sufE-like protein 2, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111496439 P...	[more]

BLAST of Cucsat.G6824.T5 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G67810.1	7.1e-53	55.22	sulfur E2	[more]
AT5G50210.1	3.6e-41	54.19	quinolinate synthase	[more]
AT4G26500.1	2.5e-13	34.33	chloroplast sulfur E	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003808	Fe-S metabolism associated domain, SufE-like	PFAM	PF02657	SufE	coord: 88..207 e-value: 3.8E-24 score: 84.9
IPR003808	Fe-S metabolism associated domain, SufE-like	PANTHER	PTHR43597	SULFUR ACCEPTOR PROTEIN CSDE	coord: 27..232
None	No IPR available	GENE3D	3.90.1010.10		coord: 78..214 e-value: 6.2E-37 score: 128.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 215..236
None	No IPR available	PANTHER	PTHR43597:SF5	SUFE-LIKE PROTEIN 2, CHLOROPLASTIC	coord: 27..232
None	No IPR available	SUPERFAMILY	82649	SufE/NifU	coord: 76..211

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G6824	Cucsat.G6824	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G6824.T5.E1	Cucsat.G6824.T5.E1	exon
Cucsat.G6824.T5.E2	Cucsat.G6824.T5.E2	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G6824.T5.C1	Cucsat.G6824.T5.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G6824.T5	Cucsat.G6824.T5-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006413	translational initiation
molecular_function	GO:0003729	mRNA binding
molecular_function	GO:0003743	translation initiation factor activity