Spg028170 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg028170
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionprotein AUXIN RESPONSE 4
Locationscaffold2: 41695223 .. 41697857 (+)
RNA-Seq ExpressionSpg028170
SyntenySpg028170
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGACCATTACCGAAGAACCAGACGAGCCAGTGCCGAATCCATCAAAGCCCAAAACCAACAAATCAAGTTCATCCTCAATGCCACCGCCGAGCACCGCCGCCGCAACCCCCAAAACCCCTTCCTCTCCGACCCCAAATCCGTTCACCTTCTGGTTCTATTTCACTTTGATCGTCTCTATCATAACCTTTTTCTTCATCTCTCTCCCTTCTCTCTCTCCCCAAGACCCCAAATCCTGGTTCTTAAGCTTACCCAACAGCCTCCGTCACCATTACTCAAAGGGCCGTTTTCTTAAGGTACAAATTAGCCCTAATTTATCTCCAATTGAAGTTTTCGCCGTTGAAAATGGCGCAAAAGGGAACGAAAATGTGGTCATTGTTCATGGGCTGGGGCTGAGCTCTTACTCCTTCCGCAAGGTTTTGGACTCATTAGGCTCCAAAGGAGTCCGGGCTGTGGCGTTTGATCTGCCCGGCAATGGCTTTTCTGACAAATCCACGGTTGAGATCGACGAGAGCACAAATGGGGTTTTAGGGAGGCTTTTGGATGTTTATAATTTGATCCATGAAAAGGGTATTTTCTGGGCGTTTGATCAGATCATTGAAACTGGGCAGATTCCTTATGAAGAAATTCAGAAACATGTTCCTAAGAGGAAGATTTTGAAGCCAATTGGGTTAGGTCCTGAAGAGATTGGCAGTATTTTGGGGCAAATCATTGATACTATTGGTTTGGCTCCTGTGCATTTGGTTCTTCATGATTCAGCTTTATCAATGGCGGGATATTGGGTTGCAGAGAATTCAGGATTTGTGAGGAGTTTAACTCTTATTGATACCTTATCGAAGCCTTCTCTTCCTTTATGGCTTTTGGAGTTGCCTGTTGTGAGAGAGGTTATTTTGGGATCTAATTTTGTGTATTCAAGGTTGATCAACTTCTGTTGTTCTGAAGGGAATGATGCTGCCTTGGATGTGGAGGCTCATAGAGTTCTTTTGAAGGGCCGGGGTGGGCGGAGGGCGGTTGTTAGCATGGGGAAGAAGTTGAACAATAGCTTTGACATTGCAGAATGGGGTGGTTTGGATGATCTGAAAAGTGTGCCGATTCAAGTTATTTGGTCCAACGGTTGGTCAAATGAATGGAGTGCGGAGGGACGCCGGGTTGCTGATGTGCTTCCACAGGCAGCTTTTGTTAAACATTCCGGTGGACGGTGGGCTCAGGTAAGGATTCATTACTTTGATTGAGAAGTTGTAATGAGTTTGGCTTGTATTTGATGTAATGAATTTGCAGTCTATATTGTGTTTTGCTGAGTTCAGAAGTATCAATAGTCCTTTTGAGGATGAGGATTTTCATGGATTTAAATGAAACATGAATGATATAGTTAGGCCTGCCTACAGAAGCTGTTTTAGCATTCGAGTTCGAATTTTCATATCTTAGAACCATCACTGTTTTTCTCTAAAGTACAATGTGAAAAGTTTGAGTGTCGTAGGGTGTATAACATGCAGCTTAACAAAATTAAAAGAAATCTGTTACTTCGAAATTTAACATTATCAAGCAGGACATGTCAAGCATGAAGGTTGTTCAAAATTTCAACAAACATCAAATTGCTAAATAGGAAGTTGGAGTCCTTGGGATTCAATTCTGCTAGTCTAGGTGAAAGTAAAACTTAGAGAGGGGCTAGGGAGGGGAGAGAAATGAGTGATGTGACACCGGATAACTTAGTGAGGAAGCATAGAGTCGGCTAATGAGGAGTTTGAGATGATGTTTCTGTCAACCAGCAGTCGAGATGGTGGAAAAAGTTTTTTTTTCTAGTTTTACAGAGAAAGTTACATAGACATATAGACTCACAGTTGTGCTTGCTGAACTGGAGATAGTAGAGAGTCGGGAAGAGAAGCTCAAGACATCAAGCCCCTGTTTAGATCATATGAGAGTCAAAACAGAAGGAAATGAAAAATGTTGACTTGTACACATATGAACACTATATTTGAGGCATCATGAGTGCTTAAGCGTTGATTGTAAGAGAATACTTAGGCTCTCGGTGCCAAGAGGGTCACTTTTTGGCTTGGAGAGAACCTACTAAAACTCTCCCTAATACATTCAGTTTGTCATCATGATTGTTTGACTTGTCCATGGTAACAAGTTGATTTCATCTGTCTTTGTGGATTGGCTCCTTGATTTTCATTTGTTTCCATAAATGGCCTCTCTTAGTGAAAGTTTGTCATCTCCTTATTTTGGTACTTGGATGTTTGATATCACATTTTGCTCGATATCTTTGTCAATGTTTCTTTAGTGGGAATCATGACTATTGATCGAACATAAGTGGGACTATTTTCATGCTAGAATTGCTACCCAGAAAGCTTGAATATATGTCTCTCTTTGAAGTTCAAATAAGCTCTTTATCTTTTTTGACCTTAGGAAGATGCAGCCAATGTCGTAACTGACAGTATAGCTCAGTTTGTGTCCTCATTACCCCCCACGGTCAGGAAAACTGTTGAAGAGCCCGAACACATTCAGAAAGTTTTTGATGTATTGGGGAACAGCGATGACCACCACCACGATCACCATCACCACCACGACCATGGCAGCCACGGCATTCATGGAGCTGGCTACATGGACGGCTATGGGCTCGGCGGTCATGCATGGTGA

mRNA sequence

ATGGTGACCATTACCGAAGAACCAGACGAGCCAGTGCCGAATCCATCAAAGCCCAAAACCAACAAATCAAGTTCATCCTCAATGCCACCGCCGAGCACCGCCGCCGCAACCCCCAAAACCCCTTCCTCTCCGACCCCAAATCCGTTCACCTTCTGGTTCTATTTCACTTTGATCGTCTCTATCATAACCTTTTTCTTCATCTCTCTCCCTTCTCTCTCTCCCCAAGACCCCAAATCCTGGTTCTTAAGCTTACCCAACAGCCTCCGTCACCATTACTCAAAGGGCCGTTTTCTTAAGGTACAAATTAGCCCTAATTTATCTCCAATTGAAGTTTTCGCCGTTGAAAATGGCGCAAAAGGGAACGAAAATGTGGTCATTGTTCATGGGCTGGGGCTGAGCTCTTACTCCTTCCGCAAGGTTTTGGACTCATTAGGCTCCAAAGGAGTCCGGGCTGTGGCGTTTGATCTGCCCGGCAATGGCTTTTCTGACAAATCCACGGTTGAGATCGACGAGAGCACAAATGGGGTTTTAGGGAGGCTTTTGGATGTTTATAATTTGATCCATGAAAAGGGTATTTTCTGGGCGTTTGATCAGATCATTGAAACTGGGCAGATTCCTTATGAAGAAATTCAGAAACATGTTCCTAAGAGGAAGATTTTGAAGCCAATTGGGTTAGGTCCTGAAGAGATTGGCAGTATTTTGGGGCAAATCATTGATACTATTGGTTTGGCTCCTGTGCATTTGGTTCTTCATGATTCAGCTTTATCAATGGCGGGATATTGGGTTGCAGAGAATTCAGGATTTGTGAGGAGTTTAACTCTTATTGATACCTTATCGAAGCCTTCTCTTCCTTTATGGCTTTTGGAGTTGCCTGTTGTGAGAGAGGTTATTTTGGGATCTAATTTTGTGTATTCAAGGTTGATCAACTTCTGTTGTTCTGAAGGGAATGATGCTGCCTTGGATGTGGAGGCTCATAGAGTTCTTTTGAAGGGCCGGGGTGGGCGGAGGGCGGTTGTTAGCATGGGGAAGAAGTTGAACAATAGCTTTGACATTGCAGAATGGGGTGGTTTGGATGATCTGAAAAGTGTGCCGATTCAAGTTATTTGGTCCAACGGTTGGTCAAATGAATGGAGTGCGGAGGGACGCCGGGTTGCTGATGTGCTTCCACAGGCAGCTTTTGTTAAACATTCCGGTGGACGGTGGGCTCAGGAAGATGCAGCCAATGTCGTAACTGACAGTATAGCTCAGTTTGTGTCCTCATTACCCCCCACGGTCAGGAAAACTGTTGAAGAGCCCGAACACATTCAGAAAGTTTTTGATGTATTGGGGAACAGCGATGACCACCACCACGATCACCATCACCACCACGACCATGGCAGCCACGGCATTCATGGAGCTGGCTACATGGACGGCTATGGGCTCGGCGGTCATGCATGGTGA

Coding sequence (CDS)

ATGGTGACCATTACCGAAGAACCAGACGAGCCAGTGCCGAATCCATCAAAGCCCAAAACCAACAAATCAAGTTCATCCTCAATGCCACCGCCGAGCACCGCCGCCGCAACCCCCAAAACCCCTTCCTCTCCGACCCCAAATCCGTTCACCTTCTGGTTCTATTTCACTTTGATCGTCTCTATCATAACCTTTTTCTTCATCTCTCTCCCTTCTCTCTCTCCCCAAGACCCCAAATCCTGGTTCTTAAGCTTACCCAACAGCCTCCGTCACCATTACTCAAAGGGCCGTTTTCTTAAGGTACAAATTAGCCCTAATTTATCTCCAATTGAAGTTTTCGCCGTTGAAAATGGCGCAAAAGGGAACGAAAATGTGGTCATTGTTCATGGGCTGGGGCTGAGCTCTTACTCCTTCCGCAAGGTTTTGGACTCATTAGGCTCCAAAGGAGTCCGGGCTGTGGCGTTTGATCTGCCCGGCAATGGCTTTTCTGACAAATCCACGGTTGAGATCGACGAGAGCACAAATGGGGTTTTAGGGAGGCTTTTGGATGTTTATAATTTGATCCATGAAAAGGGTATTTTCTGGGCGTTTGATCAGATCATTGAAACTGGGCAGATTCCTTATGAAGAAATTCAGAAACATGTTCCTAAGAGGAAGATTTTGAAGCCAATTGGGTTAGGTCCTGAAGAGATTGGCAGTATTTTGGGGCAAATCATTGATACTATTGGTTTGGCTCCTGTGCATTTGGTTCTTCATGATTCAGCTTTATCAATGGCGGGATATTGGGTTGCAGAGAATTCAGGATTTGTGAGGAGTTTAACTCTTATTGATACCTTATCGAAGCCTTCTCTTCCTTTATGGCTTTTGGAGTTGCCTGTTGTGAGAGAGGTTATTTTGGGATCTAATTTTGTGTATTCAAGGTTGATCAACTTCTGTTGTTCTGAAGGGAATGATGCTGCCTTGGATGTGGAGGCTCATAGAGTTCTTTTGAAGGGCCGGGGTGGGCGGAGGGCGGTTGTTAGCATGGGGAAGAAGTTGAACAATAGCTTTGACATTGCAGAATGGGGTGGTTTGGATGATCTGAAAAGTGTGCCGATTCAAGTTATTTGGTCCAACGGTTGGTCAAATGAATGGAGTGCGGAGGGACGCCGGGTTGCTGATGTGCTTCCACAGGCAGCTTTTGTTAAACATTCCGGTGGACGGTGGGCTCAGGAAGATGCAGCCAATGTCGTAACTGACAGTATAGCTCAGTTTGTGTCCTCATTACCCCCCACGGTCAGGAAAACTGTTGAAGAGCCCGAACACATTCAGAAAGTTTTTGATGTATTGGGGAACAGCGATGACCACCACCACGATCACCATCACCACCACGACCATGGCAGCCACGGCATTCATGGAGCTGGCTACATGGACGGCTATGGGCTCGGCGGTCATGCATGGTGA

Protein sequence

MVTITEEPDEPVPNPSKPKTNKSSSSSMPPPSTAAATPKTPSSPTPNPFTFWFYFTLIVSIITFFFISLPSLSPQDPKSWFLSLPNSLRHHYSKGRFLKVQISPNLSPIEVFAVENGAKGNENVVIVHGLGLSSYSFRKVLDSLGSKGVRAVAFDLPGNGFSDKSTVEIDESTNGVLGRLLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPKRKILKPIGLGPEEIGSILGQIIDTIGLAPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVILGSNFVYSRLINFCCSEGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNNSFDIAEWGGLDDLKSVPIQVIWSNGWSNEWSAEGRRVADVLPQAAFVKHSGGRWAQEDAANVVTDSIAQFVSSLPPTVRKTVEEPEHIQKVFDVLGNSDDHHHDHHHHHDHGSHGIHGAGYMDGYGLGGHAW
Homology
BLAST of Spg028170 vs. NCBI nr
Match: XP_038880139.1 (protein AUXIN RESPONSE 4 [Benincasa hispida])

HSP 1 Score: 873.6 bits (2256), Expect = 7.7e-250
Identity = 439/481 (91.27%), Postives = 453/481 (94.18%), Query Frame = 0

Query: 1   MVTITEEPDEPVPNPSKPKTNKSSSSSMPPPSTAAATPKTPSSPTPNPFTFWFYFTLIVS 60
           MVTITEEPDE VP PSKPK+NKSSSSS PPPS+  A PKTP SPTPNPFTFWFYFTLIVS
Sbjct: 1   MVTITEEPDESVPKPSKPKSNKSSSSSKPPPSSTTAPPKTPPSPTPNPFTFWFYFTLIVS 60

Query: 61  IITFFFISLPSLSPQDPKSWFLSLPNSLRHHYSKGRFLKVQISPNLSPIEVFAVENGAKG 120
            +TFFFISLPSLSPQDPKSWFLSLP SLR HYSKGR LKVQISPNLSPIEVFAVENG KG
Sbjct: 61  FVTFFFISLPSLSPQDPKSWFLSLPTSLRQHYSKGRLLKVQISPNLSPIEVFAVENGVKG 120

Query: 121 NENVVIVHGLGLSSYSFRKVLDSLGSKGVRAVAFDLPGNGFSDKSTVEIDESTNGVLGRL 180
           NENVVIVHGLGLSSYSFRKVLDSLGSKGVRA+AFDLPGNGFSDKST EIDES+NGVLGRL
Sbjct: 121 NENVVIVHGLGLSSYSFRKVLDSLGSKGVRALAFDLPGNGFSDKSTAEIDESSNGVLGRL 180

Query: 181 LDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPKRKILKPIGLGPEEIGSILGQIIDT 240
           LDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPKRKILKPIGLGPEEIGSILGQI+DT
Sbjct: 181 LDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPKRKILKPIGLGPEEIGSILGQIVDT 240

Query: 241 IGLAPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVILGS 300
           IGLAPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVILGS
Sbjct: 241 IGLAPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVILGS 300

Query: 301 NFVYSRLINFCCSEGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNNSFDIAEWGGLDDL 360
           NFVYSRLIN CCS+GNDA +DVEAHRVLLKGRGGRRAVVSMGKKLN+SFDI+EWGGLDDL
Sbjct: 301 NFVYSRLINLCCSKGNDAPIDVEAHRVLLKGRGGRRAVVSMGKKLNDSFDISEWGGLDDL 360

Query: 361 KSVPIQVIWSNGWSNEWSAEGRRVADVLPQAAFVKHSGGRWAQEDAANVVTDSIAQFVSS 420
           KSVP+QVIWSNGWSNEWSAEGRRVADVLPQA+FVKHSGGRWAQED A+VV DSIAQFVSS
Sbjct: 361 KSVPMQVIWSNGWSNEWSAEGRRVADVLPQASFVKHSGGRWAQEDVADVVADSIAQFVSS 420

Query: 421 LPPTVRKTVEE--PEHIQKVFDVLGNSDDHHHDHHHHHDHGSHGIHGAGYMDGYGLGGHA 480
           LPPTVRK+ EE  PEHIQKVFD  GNS     DHHHHHDHGSHGIHGAGYMDGYGLG HA
Sbjct: 421 LPPTVRKSAEETTPEHIQKVFDESGNS-----DHHHHHDHGSHGIHGAGYMDGYGLGSHA 476

BLAST of Spg028170 vs. NCBI nr
Match: XP_022969547.1 (protein AUXIN RESPONSE 4 [Cucurbita maxima])

HSP 1 Score: 855.9 bits (2210), Expect = 1.7e-244
Identity = 434/480 (90.42%), Postives = 446/480 (92.92%), Query Frame = 0

Query: 1   MVTITEEPDEPVPNPSKPKTNKSSSSSMPPP-STAAATPKTPSSPTPNPFTFWFYFTLIV 60
           MVTITEE DEPVP  SKPK NKSSSSS PPP STAAAT KT  SP PNPFTFWFYFTLIV
Sbjct: 1   MVTITEESDEPVPKASKPKPNKSSSSSKPPPSSTAAATSKTLRSPNPNPFTFWFYFTLIV 60

Query: 61  SIITFFFISLPSLSPQDPKSWFLSLPNSLRHHYSKGRFLKVQISPNLSPIEVFAVENGAK 120
           SIITFFFIS PSLSPQDPKSWFLSLP SLRHHYSKGR LKVQ+SPNLSPIEVFAVENGAK
Sbjct: 61  SIITFFFISFPSLSPQDPKSWFLSLPKSLRHHYSKGRLLKVQVSPNLSPIEVFAVENGAK 120

Query: 121 GNENVVIVHGLGLSSYSFRKVLDSLGSKGVRAVAFDLPGNGFSDKSTVEIDESTNGVLGR 180
           GNENVV+VHGLGLSSYSFRKVLDSLGSKGVRAVAFDLPGNGFSDKST EIDES+NGV GR
Sbjct: 121 GNENVVVVHGLGLSSYSFRKVLDSLGSKGVRAVAFDLPGNGFSDKSTAEIDESSNGVFGR 180

Query: 181 LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPKRKILKPIGLGPEEIGSILGQIID 240
           LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVP+RKILKPIGLGPEEIGSILGQIID
Sbjct: 181 LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPQRKILKPIGLGPEEIGSILGQIID 240

Query: 241 TIGLAPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVILG 300
           T+GLAPVHLVLHDSALSMAGYWVAENS  VRSLTLIDTLS PSLPLWLLELPV REV+LG
Sbjct: 241 TVGLAPVHLVLHDSALSMAGYWVAENSQSVRSLTLIDTLSNPSLPLWLLELPVAREVVLG 300

Query: 301 SNFVYSRLINFCCSEGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNNSFDIAEWGGLDD 360
           SNFVYS+LINFCCS+GNDAALDVEAHRVLLKGRGGRRAVVSMGKKLN+SFDIAEWGGLDD
Sbjct: 301 SNFVYSKLINFCCSKGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNHSFDIAEWGGLDD 360

Query: 361 LKSVPIQVIWSNGWSNEWSAEGRRVADVLPQAAFVKHSGGRWAQEDAANVVTDSIAQFVS 420
           LKSVPIQVIWSN WS+EWSAEG RVAD+LPQAAFVKHSGGRWAQEDAAN V DSIAQFVS
Sbjct: 361 LKSVPIQVIWSNNWSDEWSAEGSRVADLLPQAAFVKHSGGRWAQEDAANAVADSIAQFVS 420

Query: 421 SLPPTVRKTVEEPEHIQKVFDVLGNSDDHHHDHHHHHDHGSHGIHGAGYMDGYGLGGHAW 480
           SLPPTVRK VEEPEH QKVFD LGNSD     HHHHHDHGSH IHGAGYM+GYGLG  AW
Sbjct: 421 SLPPTVRKDVEEPEHTQKVFDELGNSD----QHHHHHDHGSHDIHGAGYMEGYGLGSQAW 476

BLAST of Spg028170 vs. NCBI nr
Match: KAG6588477.1 (Protein AUXIN RESPONSE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 853.6 bits (2204), Expect = 8.2e-244
Identity = 430/480 (89.58%), Postives = 447/480 (93.12%), Query Frame = 0

Query: 1   MVTITEEPDEPVPNPSKPKTNKSSSSSMPPP-STAAATPKTPSSPTPNPFTFWFYFTLIV 60
           MVTITEE DEPVP  SKPK NKSSSS+ PPP STAAAT KT  SP P+PFTFWFYFTLIV
Sbjct: 1   MVTITEESDEPVPKASKPKPNKSSSSTKPPPLSTAAATSKTLRSPNPHPFTFWFYFTLIV 60

Query: 61  SIITFFFISLPSLSPQDPKSWFLSLPNSLRHHYSKGRFLKVQISPNLSPIEVFAVENGAK 120
           SIITFFFIS+PSLSPQDPKSWFLSLP SLRHHYSKGR LKVQISPNLSPIEVFAVENGAK
Sbjct: 61  SIITFFFISVPSLSPQDPKSWFLSLPKSLRHHYSKGRLLKVQISPNLSPIEVFAVENGAK 120

Query: 121 GNENVVIVHGLGLSSYSFRKVLDSLGSKGVRAVAFDLPGNGFSDKSTVEIDESTNGVLGR 180
           GNENVV+VHGLGLSSYSFRKVLDSLGSKGVRAVAFDLPGNGFSDKST EIDE++NGV GR
Sbjct: 121 GNENVVVVHGLGLSSYSFRKVLDSLGSKGVRAVAFDLPGNGFSDKSTAEIDENSNGVFGR 180

Query: 181 LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPKRKILKPIGLGPEEIGSILGQIID 240
           LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVP+RKILKPIGLGPEEIGSILGQIID
Sbjct: 181 LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPQRKILKPIGLGPEEIGSILGQIID 240

Query: 241 TIGLAPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVILG 300
           T+GLAPVHLVLHDSALSMAGYWVAENS  VRSLTLIDTLS PSLPLWLLELPV RE++LG
Sbjct: 241 TVGLAPVHLVLHDSALSMAGYWVAENSQSVRSLTLIDTLSNPSLPLWLLELPVAREIVLG 300

Query: 301 SNFVYSRLINFCCSEGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNNSFDIAEWGGLDD 360
           SNFVYS+LINFCCS+GNDAALDVEAHRVLLKGRGGRRAVVSMGKKLN+SFDIAEWGGLDD
Sbjct: 301 SNFVYSKLINFCCSKGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNHSFDIAEWGGLDD 360

Query: 361 LKSVPIQVIWSNGWSNEWSAEGRRVADVLPQAAFVKHSGGRWAQEDAANVVTDSIAQFVS 420
           LKSVPIQVIWSN WS+EWSAEG RVAD+LPQAAFVKHSGGRWAQEDAAN + DSIAQFVS
Sbjct: 361 LKSVPIQVIWSNNWSDEWSAEGSRVADLLPQAAFVKHSGGRWAQEDAANAIVDSIAQFVS 420

Query: 421 SLPPTVRKTVEEPEHIQKVFDVLGNSDDHHHDHHHHHDHGSHGIHGAGYMDGYGLGGHAW 480
           SLPPTVRK VEEPEH QKVFD LGNSD     HHHHHDHG H IHGAGYM+GYGLG HAW
Sbjct: 421 SLPPTVRKAVEEPEHTQKVFDELGNSD----QHHHHHDHGGHDIHGAGYMEGYGLGSHAW 476

BLAST of Spg028170 vs. NCBI nr
Match: XP_022933511.1 (protein AUXIN RESPONSE 4 [Cucurbita moschata])

HSP 1 Score: 852.0 bits (2200), Expect = 2.4e-243
Identity = 430/480 (89.58%), Postives = 445/480 (92.71%), Query Frame = 0

Query: 1   MVTITEEPDEPVPNPSKPKTNKSSSSSMPPP-STAAATPKTPSSPTPNPFTFWFYFTLIV 60
           MVTITEE DEPVP  SKPK NKSSSSS PPP STAAAT KT  SP PNPFTFWFYFTLIV
Sbjct: 1   MVTITEESDEPVPKASKPKPNKSSSSSKPPPSSTAAATSKTLRSPNPNPFTFWFYFTLIV 60

Query: 61  SIITFFFISLPSLSPQDPKSWFLSLPNSLRHHYSKGRFLKVQISPNLSPIEVFAVENGAK 120
           SIITFFFIS+PSLSPQDPKSWFLSLP SLRHHYSKGR LKVQISPNLSPIEVFAVENGAK
Sbjct: 61  SIITFFFISVPSLSPQDPKSWFLSLPKSLRHHYSKGRLLKVQISPNLSPIEVFAVENGAK 120

Query: 121 GNENVVIVHGLGLSSYSFRKVLDSLGSKGVRAVAFDLPGNGFSDKSTVEIDESTNGVLGR 180
           GNENVV+VHGLGLSSYSFRKVLDSLGSKGV AVAFDLPGNGFSDKST EIDE++NGV GR
Sbjct: 121 GNENVVVVHGLGLSSYSFRKVLDSLGSKGVHAVAFDLPGNGFSDKSTAEIDENSNGVFGR 180

Query: 181 LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPKRKILKPIGLGPEEIGSILGQIID 240
           LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVP+RKILKPIGLGPEEIGSILGQIID
Sbjct: 181 LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPQRKILKPIGLGPEEIGSILGQIID 240

Query: 241 TIGLAPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVILG 300
           T+GL+PVHLVLHDSALSMAGYWVAENS  VRSLTLIDTLS PSLPLWLLELPV RE++LG
Sbjct: 241 TVGLSPVHLVLHDSALSMAGYWVAENSQSVRSLTLIDTLSNPSLPLWLLELPVAREIVLG 300

Query: 301 SNFVYSRLINFCCSEGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNNSFDIAEWGGLDD 360
           SNFVYS+LINFCCS+GNDAALDVEAHRVLLKGRGGRRAVVSMGKKLN+SFDIAEWGGLDD
Sbjct: 301 SNFVYSKLINFCCSKGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNHSFDIAEWGGLDD 360

Query: 361 LKSVPIQVIWSNGWSNEWSAEGRRVADVLPQAAFVKHSGGRWAQEDAANVVTDSIAQFVS 420
           LK VPIQVIWSN WS+EWSAEG RVAD+LPQAAFVKHSGGRWAQEDAAN V DSIAQFVS
Sbjct: 361 LKRVPIQVIWSNNWSDEWSAEGSRVADLLPQAAFVKHSGGRWAQEDAANAVADSIAQFVS 420

Query: 421 SLPPTVRKTVEEPEHIQKVFDVLGNSDDHHHDHHHHHDHGSHGIHGAGYMDGYGLGGHAW 480
           SLPPTVRK VEEPEH QKVFD LGNSD     HHHHHDHG H IHGAGYM+GYGLG HAW
Sbjct: 421 SLPPTVRKAVEEPEHTQKVFDELGNSD----QHHHHHDHGGHDIHGAGYMEGYGLGSHAW 476

BLAST of Spg028170 vs. NCBI nr
Match: XP_023530238.1 (protein AUXIN RESPONSE 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 846.7 bits (2186), Expect = 1.0e-241
Identity = 427/480 (88.96%), Postives = 444/480 (92.50%), Query Frame = 0

Query: 1   MVTITEEPDEPVPNPSKPKTNKSSSSSM-PPPSTAAATPKTPSSPTPNPFTFWFYFTLIV 60
           MVTITEE D+PVP  SKPK NKSSSS+  PPPSTAAAT KT  SP PNPFTFWFYFTLIV
Sbjct: 1   MVTITEESDDPVPKASKPKPNKSSSSTKPPPPSTAAATSKTLRSPNPNPFTFWFYFTLIV 60

Query: 61  SIITFFFISLPSLSPQDPKSWFLSLPNSLRHHYSKGRFLKVQISPNLSPIEVFAVENGAK 120
           SIITFFFIS PSLSPQDPKSWFLSLP SLRHHYSKGR LKVQ+SPNLSPIEVFAVENGAK
Sbjct: 61  SIITFFFISFPSLSPQDPKSWFLSLPKSLRHHYSKGRLLKVQVSPNLSPIEVFAVENGAK 120

Query: 121 GNENVVIVHGLGLSSYSFRKVLDSLGSKGVRAVAFDLPGNGFSDKSTVEIDESTNGVLGR 180
           GNENVV+VHGLGLSSYSFRKVLDSLGSKGVRAVAFDLPGNGFSDKST EIDE++NGV GR
Sbjct: 121 GNENVVVVHGLGLSSYSFRKVLDSLGSKGVRAVAFDLPGNGFSDKSTAEIDENSNGVFGR 180

Query: 181 LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPKRKILKPIGLGPEEIGSILGQIID 240
           LLDVYNLIH+KGIFWAFDQIIETGQIPYEEIQKHVP+RKILKPIGLGPEEIGSILGQIID
Sbjct: 181 LLDVYNLIHDKGIFWAFDQIIETGQIPYEEIQKHVPQRKILKPIGLGPEEIGSILGQIID 240

Query: 241 TIGLAPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVILG 300
           T+GLAPVHLVLHDSALSMAGYWVAENS  VRSLTLIDTLS PSLPLWLLELPV REV+LG
Sbjct: 241 TVGLAPVHLVLHDSALSMAGYWVAENSQSVRSLTLIDTLSNPSLPLWLLELPVAREVVLG 300

Query: 301 SNFVYSRLINFCCSEGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNNSFDIAEWGGLDD 360
           SNFVYS+LINFCCS+GNDAALDVEAHRVLLKGRGGRR+VVSMGKKLN+SFDIAEWGGLDD
Sbjct: 301 SNFVYSKLINFCCSKGNDAALDVEAHRVLLKGRGGRRSVVSMGKKLNHSFDIAEWGGLDD 360

Query: 361 LKSVPIQVIWSNGWSNEWSAEGRRVADVLPQAAFVKHSGGRWAQEDAANVVTDSIAQFVS 420
           LKSVPIQVIWSN WSNEWSAEG RVAD+LPQAAFVKHSGGRWAQEDAAN + DSIAQFVS
Sbjct: 361 LKSVPIQVIWSNNWSNEWSAEGSRVADLLPQAAFVKHSGGRWAQEDAANAIADSIAQFVS 420

Query: 421 SLPPTVRKTVEEPEHIQKVFDVLGNSDDHHHDHHHHHDHGSHGIHGAGYMDGYGLGGHAW 480
           SLP TVRK VEEPEH QKVFD LGNSD     HHHHHDHG H IHGAGYM+GYGLG  AW
Sbjct: 421 SLPLTVRKAVEEPEHTQKVFDELGNSD----QHHHHHDHGGHDIHGAGYMEGYGLGSQAW 476

BLAST of Spg028170 vs. ExPASy Swiss-Prot
Match: Q9FZ33 (Protein AUXIN RESPONSE 4 OS=Arabidopsis thaliana OX=3702 GN=AXR4 PE=1 SV=1)

HSP 1 Score: 475.3 bits (1222), Expect = 8.0e-133
Identity = 256/480 (53.33%), Postives = 329/480 (68.54%), Query Frame = 0

Query: 1   MVTITEEPDEPVPNPSKPKTNKSSSSSMPPPSTAAATPKTPSSPTPNPFTFWFYFTLIVS 60
           M  ITEE ++  P    P  NK   S     + + +T K P   + NPF FWFYFT++VS
Sbjct: 1   MAIITEEEED--PKTLNPPKNKPKDSDF---TKSESTMKNPKPQSQNPFPFWFYFTVVVS 60

Query: 61  IITFFFISLPSLSPQ-DPKSWFLSLPNSLRHHYSKGRFLKVQISPNLSPIEVFAVENGAK 120
           + T  FISL   S Q DP+SWFLSLP +LR HYS GR +KVQ++ N SPIEVF  E+G+ 
Sbjct: 61  LATIIFISLSLFSSQNDPRSWFLSLPPALRQHYSNGRTIKVQVNSNESPIEVFVAESGSI 120

Query: 121 GNENVVIVHGLGLSSYSFRKVLDSLGSKGVRAVAFDLPGNGFSDKSTVEI-DESTNGVLG 180
             E VVIVHGLGLSS++F++++ SLGSKG+ +VA DLPGNGFSDKS V I  +   G + 
Sbjct: 121 HTETVVIVHGLGLSSFAFKEMIQSLGSKGIHSVAIDLPGNGFSDKSMVVIGGDREIGFVA 180

Query: 181 RLLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQK-HVPKRKILKPIGLGPEEIGSILGQI 240
           R+ +VY LI EKG+FWAFDQ+IETG +PYEEI K    KR+  K I LG EE   +LGQ+
Sbjct: 181 RVKEVYGLIQEKGVFWAFDQMIETGDLPYEEIIKLQNSKRRSFKAIELGSEETARVLGQV 240

Query: 241 IDTIGLAPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVI 300
           IDT+GLAPVHLVLHDSAL +A  WV+EN   VRS+TLID+   P+LPLW+L +P +RE++
Sbjct: 241 IDTLGLAPVHLVLHDSALGLASNWVSENWQSVRSVTLIDSSISPALPLWVLNVPGIREIL 300

Query: 301 LGSNFVYSRLINFCCSEGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNNSFDIAEWGGL 360
           L  +F + +L++F CS+    + D++AHR+LLKGR GR AVV+   KLN+SFDIA+WG  
Sbjct: 301 LAFSFGFEKLVSFRCSKEMTLS-DIDAHRILLKGRNGREAVVASLNKLNHSFDIAQWGNS 360

Query: 361 DDLKSVPIQVIWSNGWSNEWSAEGRRVADVLPQAAFVKHSGGRWAQEDAANVVTDSIAQF 420
           D +  +P+QVIWS+  S EWS EG+RVA  LP+A FV HSG RW QE  +  + D I++F
Sbjct: 361 DGINGIPMQVIWSSEASKEWSDEGQRVAKALPKAKFVTHSGSRWPQESKSGELADYISEF 420

Query: 421 VSSLPPTVRKTVEE--PEHIQKVFDVLGNSDDHHHDHHHHHDHGSHGIHGAGYMDGYGLG 476
           VS LP ++R+  EE  PE +QKV +     DDH H H H H H       AGY D YGLG
Sbjct: 421 VSLLPKSIRRVAEEPIPEEVQKVLEEAKAGDDHDHHHGHGHAH-------AGYSDAYGLG 467

BLAST of Spg028170 vs. ExPASy TrEMBL
Match: A0A6J1HWL6 (protein AUXIN RESPONSE 4 OS=Cucurbita maxima OX=3661 GN=LOC111468529 PE=4 SV=1)

HSP 1 Score: 855.9 bits (2210), Expect = 8.0e-245
Identity = 434/480 (90.42%), Postives = 446/480 (92.92%), Query Frame = 0

Query: 1   MVTITEEPDEPVPNPSKPKTNKSSSSSMPPP-STAAATPKTPSSPTPNPFTFWFYFTLIV 60
           MVTITEE DEPVP  SKPK NKSSSSS PPP STAAAT KT  SP PNPFTFWFYFTLIV
Sbjct: 1   MVTITEESDEPVPKASKPKPNKSSSSSKPPPSSTAAATSKTLRSPNPNPFTFWFYFTLIV 60

Query: 61  SIITFFFISLPSLSPQDPKSWFLSLPNSLRHHYSKGRFLKVQISPNLSPIEVFAVENGAK 120
           SIITFFFIS PSLSPQDPKSWFLSLP SLRHHYSKGR LKVQ+SPNLSPIEVFAVENGAK
Sbjct: 61  SIITFFFISFPSLSPQDPKSWFLSLPKSLRHHYSKGRLLKVQVSPNLSPIEVFAVENGAK 120

Query: 121 GNENVVIVHGLGLSSYSFRKVLDSLGSKGVRAVAFDLPGNGFSDKSTVEIDESTNGVLGR 180
           GNENVV+VHGLGLSSYSFRKVLDSLGSKGVRAVAFDLPGNGFSDKST EIDES+NGV GR
Sbjct: 121 GNENVVVVHGLGLSSYSFRKVLDSLGSKGVRAVAFDLPGNGFSDKSTAEIDESSNGVFGR 180

Query: 181 LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPKRKILKPIGLGPEEIGSILGQIID 240
           LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVP+RKILKPIGLGPEEIGSILGQIID
Sbjct: 181 LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPQRKILKPIGLGPEEIGSILGQIID 240

Query: 241 TIGLAPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVILG 300
           T+GLAPVHLVLHDSALSMAGYWVAENS  VRSLTLIDTLS PSLPLWLLELPV REV+LG
Sbjct: 241 TVGLAPVHLVLHDSALSMAGYWVAENSQSVRSLTLIDTLSNPSLPLWLLELPVAREVVLG 300

Query: 301 SNFVYSRLINFCCSEGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNNSFDIAEWGGLDD 360
           SNFVYS+LINFCCS+GNDAALDVEAHRVLLKGRGGRRAVVSMGKKLN+SFDIAEWGGLDD
Sbjct: 301 SNFVYSKLINFCCSKGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNHSFDIAEWGGLDD 360

Query: 361 LKSVPIQVIWSNGWSNEWSAEGRRVADVLPQAAFVKHSGGRWAQEDAANVVTDSIAQFVS 420
           LKSVPIQVIWSN WS+EWSAEG RVAD+LPQAAFVKHSGGRWAQEDAAN V DSIAQFVS
Sbjct: 361 LKSVPIQVIWSNNWSDEWSAEGSRVADLLPQAAFVKHSGGRWAQEDAANAVADSIAQFVS 420

Query: 421 SLPPTVRKTVEEPEHIQKVFDVLGNSDDHHHDHHHHHDHGSHGIHGAGYMDGYGLGGHAW 480
           SLPPTVRK VEEPEH QKVFD LGNSD     HHHHHDHGSH IHGAGYM+GYGLG  AW
Sbjct: 421 SLPPTVRKDVEEPEHTQKVFDELGNSD----QHHHHHDHGSHDIHGAGYMEGYGLGSQAW 476

BLAST of Spg028170 vs. ExPASy TrEMBL
Match: A0A6J1EZ91 (protein AUXIN RESPONSE 4 OS=Cucurbita moschata OX=3662 GN=LOC111440912 PE=4 SV=1)

HSP 1 Score: 852.0 bits (2200), Expect = 1.2e-243
Identity = 430/480 (89.58%), Postives = 445/480 (92.71%), Query Frame = 0

Query: 1   MVTITEEPDEPVPNPSKPKTNKSSSSSMPPP-STAAATPKTPSSPTPNPFTFWFYFTLIV 60
           MVTITEE DEPVP  SKPK NKSSSSS PPP STAAAT KT  SP PNPFTFWFYFTLIV
Sbjct: 1   MVTITEESDEPVPKASKPKPNKSSSSSKPPPSSTAAATSKTLRSPNPNPFTFWFYFTLIV 60

Query: 61  SIITFFFISLPSLSPQDPKSWFLSLPNSLRHHYSKGRFLKVQISPNLSPIEVFAVENGAK 120
           SIITFFFIS+PSLSPQDPKSWFLSLP SLRHHYSKGR LKVQISPNLSPIEVFAVENGAK
Sbjct: 61  SIITFFFISVPSLSPQDPKSWFLSLPKSLRHHYSKGRLLKVQISPNLSPIEVFAVENGAK 120

Query: 121 GNENVVIVHGLGLSSYSFRKVLDSLGSKGVRAVAFDLPGNGFSDKSTVEIDESTNGVLGR 180
           GNENVV+VHGLGLSSYSFRKVLDSLGSKGV AVAFDLPGNGFSDKST EIDE++NGV GR
Sbjct: 121 GNENVVVVHGLGLSSYSFRKVLDSLGSKGVHAVAFDLPGNGFSDKSTAEIDENSNGVFGR 180

Query: 181 LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPKRKILKPIGLGPEEIGSILGQIID 240
           LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVP+RKILKPIGLGPEEIGSILGQIID
Sbjct: 181 LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPQRKILKPIGLGPEEIGSILGQIID 240

Query: 241 TIGLAPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVILG 300
           T+GL+PVHLVLHDSALSMAGYWVAENS  VRSLTLIDTLS PSLPLWLLELPV RE++LG
Sbjct: 241 TVGLSPVHLVLHDSALSMAGYWVAENSQSVRSLTLIDTLSNPSLPLWLLELPVAREIVLG 300

Query: 301 SNFVYSRLINFCCSEGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNNSFDIAEWGGLDD 360
           SNFVYS+LINFCCS+GNDAALDVEAHRVLLKGRGGRRAVVSMGKKLN+SFDIAEWGGLDD
Sbjct: 301 SNFVYSKLINFCCSKGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNHSFDIAEWGGLDD 360

Query: 361 LKSVPIQVIWSNGWSNEWSAEGRRVADVLPQAAFVKHSGGRWAQEDAANVVTDSIAQFVS 420
           LK VPIQVIWSN WS+EWSAEG RVAD+LPQAAFVKHSGGRWAQEDAAN V DSIAQFVS
Sbjct: 361 LKRVPIQVIWSNNWSDEWSAEGSRVADLLPQAAFVKHSGGRWAQEDAANAVADSIAQFVS 420

Query: 421 SLPPTVRKTVEEPEHIQKVFDVLGNSDDHHHDHHHHHDHGSHGIHGAGYMDGYGLGGHAW 480
           SLPPTVRK VEEPEH QKVFD LGNSD     HHHHHDHG H IHGAGYM+GYGLG HAW
Sbjct: 421 SLPPTVRKAVEEPEHTQKVFDELGNSD----QHHHHHDHGGHDIHGAGYMEGYGLGSHAW 476

BLAST of Spg028170 vs. ExPASy TrEMBL
Match: A0A5D3CG86 (Protein AUXIN RESPONSE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G003850 PE=3 SV=1)

HSP 1 Score: 838.6 bits (2165), Expect = 1.3e-239
Identity = 429/481 (89.19%), Postives = 446/481 (92.72%), Query Frame = 0

Query: 1   MVTITEEPDEPVPNPSKPKTNK-SSSSSMPPPSTAAATPKTPSSPTPNPFTFWFYFTLIV 60
           MVTITEEPDEPV  PS+P+ NK +SSSS P  S+A AT KTP SPTPNPFTFWFYF LIV
Sbjct: 1   MVTITEEPDEPVRKPSEPQPNKPTSSSSKPLSSSATATHKTPPSPTPNPFTFWFYFILIV 60

Query: 61  SIITFFFISLPSLSPQDPKSWFLSLPNSLRHHYSKGRFLKVQISPNLSPIEVFAVENGAK 120
           SIIT+FFISLPSLSP DPKSWFLSLPNSLRHHYSKGR LKVQIS NLSPIEVFAVENGAK
Sbjct: 61  SIITYFFISLPSLSPPDPKSWFLSLPNSLRHHYSKGRLLKVQISGNLSPIEVFAVENGAK 120

Query: 121 GNENVVIVHGLGLSSYSFRKVLDSLGSKGVRAVAFDLPGNGFSDKSTVEIDESTNGVLGR 180
           GNENVVIVHGLGLSSYSFRKVLDSLGSKGVRA+AFDLPGNGFSDKST EIDES+NGVLGR
Sbjct: 121 GNENVVIVHGLGLSSYSFRKVLDSLGSKGVRALAFDLPGNGFSDKSTAEIDESSNGVLGR 180

Query: 181 LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPKRKILKPIGLGPEEIGSILGQIID 240
           LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPKRKILKPIGLGPEEIGSILGQIID
Sbjct: 181 LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPKRKILKPIGLGPEEIGSILGQIID 240

Query: 241 TIGLAPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVILG 300
           TIGLAPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVILG
Sbjct: 241 TIGLAPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVILG 300

Query: 301 SNFVYSRLINFCCSEGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNNSFDIAEWGGLDD 360
           SNFVYSRLIN CCS+GNDA LDVEAHRVLLKG GGRRAVVSMGKKLN+SFDIAEWGGLDD
Sbjct: 301 SNFVYSRLINLCCSKGNDAPLDVEAHRVLLKGLGGRRAVVSMGKKLNDSFDIAEWGGLDD 360

Query: 361 LKSVPIQVIWSNGWSNEWSAEGRRVADVLPQAAFVKHSGGRWAQEDAANVVTDSIAQFVS 420
           LKSVP+QVIWSNGWSNEWS EGRRVA+VLPQA+FV+HSGGRWAQED A+VV DSI+QF+S
Sbjct: 361 LKSVPMQVIWSNGWSNEWSTEGRRVAEVLPQASFVEHSGGRWAQEDVADVVADSISQFIS 420

Query: 421 SLPPTVRKTVE-EPEHIQKVFDVLGNSDDHHHDHHHHHDHGSHGIHGAGYMDGYGLGGHA 480
           SLPPTVRKT E  PEHI +VFD   NSD     HHHHHDHGSHGIHGAGYMDGYGLG  A
Sbjct: 421 SLPPTVRKTEEPTPEHIHEVFDQSMNSD----HHHHHHDHGSHGIHGAGYMDGYGLGSQA 477

BLAST of Spg028170 vs. ExPASy TrEMBL
Match: A0A1S3BP26 (protein AUXIN RESPONSE 4 OS=Cucumis melo OX=3656 GN=LOC103492173 PE=4 SV=1)

HSP 1 Score: 838.6 bits (2165), Expect = 1.3e-239
Identity = 429/481 (89.19%), Postives = 446/481 (92.72%), Query Frame = 0

Query: 1   MVTITEEPDEPVPNPSKPKTNK-SSSSSMPPPSTAAATPKTPSSPTPNPFTFWFYFTLIV 60
           MVTITEEPDEPV  PS+P+ NK +SSSS P  S+A AT KTP SPTPNPFTFWFYF LIV
Sbjct: 1   MVTITEEPDEPVRKPSEPQPNKPTSSSSKPLSSSATATHKTPPSPTPNPFTFWFYFILIV 60

Query: 61  SIITFFFISLPSLSPQDPKSWFLSLPNSLRHHYSKGRFLKVQISPNLSPIEVFAVENGAK 120
           SIIT+FFISLPSLSP DPKSWFLSLPNSLRHHYSKGR LKVQIS NLSPIEVFAVENGAK
Sbjct: 61  SIITYFFISLPSLSPPDPKSWFLSLPNSLRHHYSKGRLLKVQISGNLSPIEVFAVENGAK 120

Query: 121 GNENVVIVHGLGLSSYSFRKVLDSLGSKGVRAVAFDLPGNGFSDKSTVEIDESTNGVLGR 180
           GNENVVIVHGLGLSSYSFRKVLDSLGSKGVRA+AFDLPGNGFSDKST EIDES+NGVLGR
Sbjct: 121 GNENVVIVHGLGLSSYSFRKVLDSLGSKGVRALAFDLPGNGFSDKSTAEIDESSNGVLGR 180

Query: 181 LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPKRKILKPIGLGPEEIGSILGQIID 240
           LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPKRKILKPIGLGPEEIGSILGQIID
Sbjct: 181 LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPKRKILKPIGLGPEEIGSILGQIID 240

Query: 241 TIGLAPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVILG 300
           TIGLAPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVILG
Sbjct: 241 TIGLAPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVILG 300

Query: 301 SNFVYSRLINFCCSEGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNNSFDIAEWGGLDD 360
           SNFVYSRLIN CCS+GNDA LDVEAHRVLLKG GGRRAVVSMGKKLN+SFDIAEWGGLDD
Sbjct: 301 SNFVYSRLINLCCSKGNDAPLDVEAHRVLLKGLGGRRAVVSMGKKLNDSFDIAEWGGLDD 360

Query: 361 LKSVPIQVIWSNGWSNEWSAEGRRVADVLPQAAFVKHSGGRWAQEDAANVVTDSIAQFVS 420
           LKSVP+QVIWSNGWSNEWS EGRRVA+VLPQA+FV+HSGGRWAQED A+VV DSI+QF+S
Sbjct: 361 LKSVPMQVIWSNGWSNEWSTEGRRVAEVLPQASFVEHSGGRWAQEDVADVVADSISQFIS 420

Query: 421 SLPPTVRKTVE-EPEHIQKVFDVLGNSDDHHHDHHHHHDHGSHGIHGAGYMDGYGLGGHA 480
           SLPPTVRKT E  PEHI +VFD   NSD     HHHHHDHGSHGIHGAGYMDGYGLG  A
Sbjct: 421 SLPPTVRKTEEPTPEHIHEVFDQSMNSD----HHHHHHDHGSHGIHGAGYMDGYGLGSQA 477

BLAST of Spg028170 vs. ExPASy TrEMBL
Match: A0A5A7U9L3 (Protein AUXIN RESPONSE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold761G00110 PE=3 SV=1)

HSP 1 Score: 833.6 bits (2152), Expect = 4.3e-238
Identity = 427/481 (88.77%), Postives = 443/481 (92.10%), Query Frame = 0

Query: 1   MVTITEEPDEPVPNPSKPKTNK-SSSSSMPPPSTAAATPKTPSSPTPNPFTFWFYFTLIV 60
           MVTITEEPDEPV  PS P+ NK +SSSS P  S+A AT KTP SPTPNPFTFWFYF LIV
Sbjct: 1   MVTITEEPDEPVRKPSGPQPNKPTSSSSKPLSSSATATHKTPPSPTPNPFTFWFYFILIV 60

Query: 61  SIITFFFISLPSLSPQDPKSWFLSLPNSLRHHYSKGRFLKVQISPNLSPIEVFAVENGAK 120
           SIIT+FFISLPSLSP DPKSWFLSLPNSLRHHYSKGR LKVQIS NLSPIEVFAVENGAK
Sbjct: 61  SIITYFFISLPSLSPPDPKSWFLSLPNSLRHHYSKGRLLKVQISGNLSPIEVFAVENGAK 120

Query: 121 GNENVVIVHGLGLSSYSFRKVLDSLGSKGVRAVAFDLPGNGFSDKSTVEIDESTNGVLGR 180
           GNENVVIVHGLGLSSYSFRKVLDSLGSKGVRA+AFDLPGNGFSDKST EIDES+NGVLGR
Sbjct: 121 GNENVVIVHGLGLSSYSFRKVLDSLGSKGVRALAFDLPGNGFSDKSTAEIDESSNGVLGR 180

Query: 181 LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPKRKILKPIGLGPEEIGSILGQIID 240
           LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPKRKILKPIGLGPEEIGSILGQIID
Sbjct: 181 LLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQKHVPKRKILKPIGLGPEEIGSILGQIID 240

Query: 241 TIGLAPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVILG 300
           TIGL PVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVILG
Sbjct: 241 TIGLTPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVILG 300

Query: 301 SNFVYSRLINFCCSEGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNNSFDIAEWGGLDD 360
           SNFVYSRLI  CCS+GNDA LDVEAHRVLLKG GGRRAVVSMGKKLN+SFDIAEWGGLDD
Sbjct: 301 SNFVYSRLIKLCCSKGNDAPLDVEAHRVLLKGLGGRRAVVSMGKKLNDSFDIAEWGGLDD 360

Query: 361 LKSVPIQVIWSNGWSNEWSAEGRRVADVLPQAAFVKHSGGRWAQEDAANVVTDSIAQFVS 420
           LKSVP+QVIWSNGWSNEWS EGRRVA+VLPQA+FV+HSGGRWAQED A+VV DSI+QF+S
Sbjct: 361 LKSVPMQVIWSNGWSNEWSTEGRRVAEVLPQASFVEHSGGRWAQEDVADVVADSISQFIS 420

Query: 421 SLPPTVRKTVE-EPEHIQKVFDVLGNSDDHHHDHHHHHDHGSHGIHGAGYMDGYGLGGHA 480
           SLPPTVRKT E  PEHI +VFD   NSD     HHHHHDHGSHGIHGAGYMDGYGLG  A
Sbjct: 421 SLPPTVRKTEEPTPEHIHEVFDQSMNSD----HHHHHHDHGSHGIHGAGYMDGYGLGSQA 477

BLAST of Spg028170 vs. TAIR 10
Match: AT1G54990.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 475.3 bits (1222), Expect = 5.7e-134
Identity = 256/480 (53.33%), Postives = 329/480 (68.54%), Query Frame = 0

Query: 1   MVTITEEPDEPVPNPSKPKTNKSSSSSMPPPSTAAATPKTPSSPTPNPFTFWFYFTLIVS 60
           M  ITEE ++  P    P  NK   S     + + +T K P   + NPF FWFYFT++VS
Sbjct: 1   MAIITEEEED--PKTLNPPKNKPKDSDF---TKSESTMKNPKPQSQNPFPFWFYFTVVVS 60

Query: 61  IITFFFISLPSLSPQ-DPKSWFLSLPNSLRHHYSKGRFLKVQISPNLSPIEVFAVENGAK 120
           + T  FISL   S Q DP+SWFLSLP +LR HYS GR +KVQ++ N SPIEVF  E+G+ 
Sbjct: 61  LATIIFISLSLFSSQNDPRSWFLSLPPALRQHYSNGRTIKVQVNSNESPIEVFVAESGSI 120

Query: 121 GNENVVIVHGLGLSSYSFRKVLDSLGSKGVRAVAFDLPGNGFSDKSTVEI-DESTNGVLG 180
             E VVIVHGLGLSS++F++++ SLGSKG+ +VA DLPGNGFSDKS V I  +   G + 
Sbjct: 121 HTETVVIVHGLGLSSFAFKEMIQSLGSKGIHSVAIDLPGNGFSDKSMVVIGGDREIGFVA 180

Query: 181 RLLDVYNLIHEKGIFWAFDQIIETGQIPYEEIQK-HVPKRKILKPIGLGPEEIGSILGQI 240
           R+ +VY LI EKG+FWAFDQ+IETG +PYEEI K    KR+  K I LG EE   +LGQ+
Sbjct: 181 RVKEVYGLIQEKGVFWAFDQMIETGDLPYEEIIKLQNSKRRSFKAIELGSEETARVLGQV 240

Query: 241 IDTIGLAPVHLVLHDSALSMAGYWVAENSGFVRSLTLIDTLSKPSLPLWLLELPVVREVI 300
           IDT+GLAPVHLVLHDSAL +A  WV+EN   VRS+TLID+   P+LPLW+L +P +RE++
Sbjct: 241 IDTLGLAPVHLVLHDSALGLASNWVSENWQSVRSVTLIDSSISPALPLWVLNVPGIREIL 300

Query: 301 LGSNFVYSRLINFCCSEGNDAALDVEAHRVLLKGRGGRRAVVSMGKKLNNSFDIAEWGGL 360
           L  +F + +L++F CS+    + D++AHR+LLKGR GR AVV+   KLN+SFDIA+WG  
Sbjct: 301 LAFSFGFEKLVSFRCSKEMTLS-DIDAHRILLKGRNGREAVVASLNKLNHSFDIAQWGNS 360

Query: 361 DDLKSVPIQVIWSNGWSNEWSAEGRRVADVLPQAAFVKHSGGRWAQEDAANVVTDSIAQF 420
           D +  +P+QVIWS+  S EWS EG+RVA  LP+A FV HSG RW QE  +  + D I++F
Sbjct: 361 DGINGIPMQVIWSSEASKEWSDEGQRVAKALPKAKFVTHSGSRWPQESKSGELADYISEF 420

Query: 421 VSSLPPTVRKTVEE--PEHIQKVFDVLGNSDDHHHDHHHHHDHGSHGIHGAGYMDGYGLG 476
           VS LP ++R+  EE  PE +QKV +     DDH H H H H H       AGY D YGLG
Sbjct: 421 VSLLPKSIRRVAEEPIPEEVQKVLEEAKAGDDHDHHHGHGHAH-------AGYSDAYGLG 467

BLAST of Spg028170 vs. TAIR 10
Match: AT4G12830.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 50.4 bits (119), Expect = 4.5e-06
Identity = 25/53 (47.17%), Postives = 36/53 (67.92%), Query Frame = 0

Query: 112 FAVENGAKGNENVVIVHGLGLSSYSFRKVLDSLGSKGVRAVAFDLPGNGFSDK 165
           F+VE+G+  +  V+++HG    +YS+RK +  L SK  RA+AFD  G GFSDK
Sbjct: 124 FSVESGSVDSPPVILIHGFPSQAYSYRKTIPVL-SKNYRAIAFDWLGFGFSDK 175

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038880139.17.7e-25091.27protein AUXIN RESPONSE 4 [Benincasa hispida][more]
XP_022969547.11.7e-24490.42protein AUXIN RESPONSE 4 [Cucurbita maxima][more]
KAG6588477.18.2e-24489.58Protein AUXIN RESPONSE 4, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022933511.12.4e-24389.58protein AUXIN RESPONSE 4 [Cucurbita moschata][more]
XP_023530238.11.0e-24188.96protein AUXIN RESPONSE 4 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9FZ338.0e-13353.33Protein AUXIN RESPONSE 4 OS=Arabidopsis thaliana OX=3702 GN=AXR4 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1HWL68.0e-24590.42protein AUXIN RESPONSE 4 OS=Cucurbita maxima OX=3661 GN=LOC111468529 PE=4 SV=1[more]
A0A6J1EZ911.2e-24389.58protein AUXIN RESPONSE 4 OS=Cucurbita moschata OX=3662 GN=LOC111440912 PE=4 SV=1[more]
A0A5D3CG861.3e-23989.19Protein AUXIN RESPONSE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A1S3BP261.3e-23989.19protein AUXIN RESPONSE 4 OS=Cucumis melo OX=3656 GN=LOC103492173 PE=4 SV=1[more]
A0A5A7U9L34.3e-23888.77Protein AUXIN RESPONSE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol... [more]
Match NameE-valueIdentityDescription
AT1G54990.15.7e-13453.33alpha/beta-Hydrolases superfamily protein [more]
AT4G12830.14.5e-0647.17alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 105..420
e-value: 3.5E-24
score: 88.1
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 108..419
IPR000073Alpha/beta hydrolase fold-1PFAMPF00561Abhydrolase_1coord: 124..280
e-value: 3.6E-8
score: 33.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..43
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 443..463
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 16..33
NoneNo IPR availablePANTHERPTHR43329:SF81PROTEIN AUXIN RESPONSE 4coord: 37..459
NoneNo IPR availablePANTHERPTHR43329EPOXIDE HYDROLASEcoord: 37..459

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg028170.1Spg028170.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009926 auxin polar transport
biological_process GO:0007049 cell cycle
biological_process GO:0051301 cell division
biological_process GO:0009733 response to auxin
biological_process GO:0009612 response to mechanical stimulus
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016787 hydrolase activity