Sed0020275 (gene) Chayote v1

Overview
NameSed0020275
Typegene
OrganismSechium edule (Chayote v1)
DescriptionReplication protein A 32 kDa subunit B-like
LocationLG12: 32546121 .. 32549447 (-)
RNA-Seq ExpressionSed0020275
SyntenySed0020275
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTCTTCAGTTGTACCGTAACAGTCGATTCGACGGCAACGCTTCCTACTCCGGCGGTGGATTCCGCCGTCTCAGACCACTCAGGCCCCGATCATCCATTCTTTCCGGCGAAAGTACGTTCTATCTCGATATATAACATTAACTCTATATCGCTTTCCATTCATTTCTGTGTTGGTTTTTAGTCTCCGCGGTTTGGATGAATCACATGCGTTTTAACCTATTTTTCCTCGCTGAAATCGTTATAGCAGTGAAATGATTCGTGCTCTGATTGTGATTGCTAGTTTGATGGACCTAATTCATTTTTCTTTTGGATTTGATGTCTGATTCATTTAATTGAAGTATATGGTTGGTTTGATGACATTTTTGCCTCTTCGTTTTCGTTTTCCGCGGGATTTGATTGTTTTGATTTTGTTGGAATTGGTTATTCATGTTTTTACCTCTTGTGTTTCCTATTCTATCGATGAATTGAAGTGTTATATTTTTGTGTCTGGTTTGAAGAATCGAGATGTCCAGACATTGCTTCCATTAACAATGAAGCAGATAAACAGCGCATTTGCCGTCTAGTGATGATAAATCAAAGTGTGTTTTGTTGATTTTTATTCCTTTAGTTGCTCTATTTTCAGGTTAATGCTGTAGGGATGATGCGCAATAGAGCAGGGAGAGTTACTGATTTGACTTTGCACTGGATGATGGGATTTGGCTGATTGACTCTGGCAAATGGTTTGTTCTTTTGTTCCCTTGTTGATATTGATTTTGTTTGGTGTATCTATAGGGTCAATGAAGCTATAGACTTAAATGAAGTTGAGGGAATATTGTAATGTTCTTTATACCCTGTCTTGGTACAATTTAAAATATTCCCTCAAAACTGTATTCATATATAGGTGCATTGTTCTGCTTATACTATAGGTGTTTGTATTTATTTTTACCAGCCATGGGATGATTAAGTTTGACGGGCTAATTTGCATTTTACAGAAAAATGAATGATATACCTTTATTCTAGGATACTTTGGAGAACACAAATACCTCATATTTATCTCAATCGTAATGAGATAACAAGTATTGATGGGAAATGGAGAATTTCTATTTTATTTTCCTTCTTTCGTAATTTTTATTTAAACAACATTTTCTAATCAGGGCATGTGCATCTTAATTGGTGTTGTTCTATCCCCTCTATGATTTATCCACATTAATATCTTGACGGAATAGTATTTTGTAATGTTTCGATAGAATTGTAACTTTTCATACATCAATGAAGGTTTATGTTTCTTATAAAAAAATACATGGCCATCTTCATTAGTATTTGTTGAGCATGTAATTGTCAACTAGTGGATTGAACATATAACTTGGTGACTTGAATATTTTTTGGATTCTATTTTCAGCTCTCTTACTCACTTGAAATGTAAATTACCAATTTTAGTATGGATGATTAGTCTCTCTATGTGTATCATAATCATGACATTAATTTTAGCTAAAGGAGGAGAGATGGCAGGCTAGATTTTTGTTACTTCCTTAAAAATTTTCATCAAGTTGCAATTACCATTCACTAGAAAGTCTCTTTTAGCTGCGCTGGACTATCATTTGTGTTTGCCCTGAAATTACGTCTTGAGAGGTTTTGATCCTTTGGGAATGGCTCTGTTTATTTTGAATAAGTGCTGCATTGCATCATCTCGAAACTTTAACTCCCTTTAGCTTATGTCATTTAAATAAGATTCTTGTGTTTCGGGGATGGTATGTATGTCCGGGTCCATGGCCACATGAAAAGCTTCCAGGGCTGGGGTGACTTGGAATATCTTTTCGATCAGGTATAATTTACTATAGTTTTCCCTTAGTTCTTTTTGGGGGTTTTCTTGGTTTTGTTATTTCAAAAGGATTTGAGTTTTTTTTAAAAAATATATTTATTCAGTACAATAGGGGATTGGAAAATTTGAACCACATATCTCTTGGTCAGTAATACATCGTTATGCTAGCTGAACTATGTTCACTTTGGCTAGAGAGGATTTGAGTTATGTTAACTGTATTAATTCAATTCTTGAACTATGGTGGATGTGAATTTATGCTTATATTTTAGGTTTAAGCAATGGAATTGAGACGAGGCACATTATGGAATGAAAGTTTATTTATGAGTATTCTATTGTTAAAATTTTTGATTATTTTGTAACGTATCGTTGGAGGAGCATCTCTTTTCCACCACTTGTAGTCATTGTGGTGCTTGTTTTTGTTACTTGTGTGCGTAATTTTTGCCATATTGCTACGGGAGTTTAGATATAGAATATTTGGTTTCCAGGCCTGTGACCAACTATAATGAGATCACGATTCACTTCATCGAACGCATATATGTTAATTTTAAAACACCAGGTTACGGGAATGAATCTAGCCATTTCAAATTTTACTTAATATTTTGATTTGGTGAAAAGTAATATTTCATGCCTTACATATGGGGTCTACTTAGGATGGTATATCGTTTGAGAAGGTACTTAAAAATAGTATGTCTTTTGAGATGGAAAACAGAGAGTGAGACGTTTTGTGCAAGGAAGAGCTATGCTAAATCTCTCTTTCTACTATTGTGATTAATATAACGTATCTTTGAATTGAAAGGGTTCGTATAATTATGTTGGACCCTGAATATTTTGAGCGACGGCACCAACAGTCAGGCAAGTTTCCCTTGGGTTTGCGTCCAGGGTTTTTAATGGGAGCATTATTAGCATGACATGAAATTTCATTTGCCTAACATTCCATATTTCCTTTGTATAAACTGCCTTATTAGTCTACATTGATCACTTTCTGCAGTTGATGAGTGTTTTTTTTGGGGTGTAAAACAAAGTTTGGAATGCTCCCTTACTATTTTCTTCCCTGCTAAACTATTTCTTTAACTTTTAATTTTTTTTATTAGTATTGAGGATCTCACATTGGAAAGGTAGGGATAAGACTTTTGCATTTTTAATAAGATGCATGAGCTACTCTTCTCATCGCCAATTGGTTTTAAGATGGAATCTCATGTTTATCTAATATAGTATTTACCCATCAAACTCAAACGGGCATTTGGTAAAATGAAAAAAGAATTGGAATACAATCCAAAAATGGAACCAAAAGAGATGTCCTCTTGATGAGATATGTATATGTTTGAGGATCCCACATTGTAAAGATAGAAAGATGACCTACATTTTAAATTTGAATATAAGATGCATGGGTTATTTTGCAGTCTAGCAATGAAAAATCTAAAAGAGGAAAGCTTAACCTACTCAACAACTGATGATTACCACTTCAAATCAACTGCTAATGGATTAACATCTCTGTTATGGTGTACTAATTATTCAAGGCAAGTTTAG

mRNA sequence

ATGTTCTTCAGTTGTACCGTAACAGTCGATTCGACGGCAACGCTTCCTACTCCGGCGGTGGATTCCGCCGTCTCAGACCACTCAGGCCCCGATCATCCATTCTTTCCGGCGAAAGTTAATGCTGTAGGGATGATGCGCAATAGAGCAGGGAGAGTTACTGATTTGACTTTGGTCAATGAAGCTATAGACTTAAATGAAGTTGAGGGAATATTATTCTTGTGTTTCGGGGATGGTATGTATGTCCGGGTCCATGGCCACATGAAAAGCTTCCAGGGCTGGGAATATTTGGTTTCCAGGCCTGTGACCAACTATAATGAGATCACGATTCACTTCATCGAACGCATATATGTTAATTTTAAAACACCAGTCTACATTGATCACTTTCTGCAGTTGATGAGTGTTTTTTTTGGGGTAATTGGAATACAATCCAAAAATGGAACCAAAAGAGATGTCCTCTTGATGAGATATATGCATGGGTTATTTTGCAGTCTAGCAATGAAAAATCTAAAAGAGGAAAGCTTAACCTACTCAACAACTGATGATTACCACTTCAAATCAACTGCTAATGGATTAACATCTCTGTTATGGTGTACTAATTATTCAAGGCAAGTTTAG

Coding sequence (CDS)

ATGTTCTTCAGTTGTACCGTAACAGTCGATTCGACGGCAACGCTTCCTACTCCGGCGGTGGATTCCGCCGTCTCAGACCACTCAGGCCCCGATCATCCATTCTTTCCGGCGAAAGTTAATGCTGTAGGGATGATGCGCAATAGAGCAGGGAGAGTTACTGATTTGACTTTGGTCAATGAAGCTATAGACTTAAATGAAGTTGAGGGAATATTATTCTTGTGTTTCGGGGATGGTATGTATGTCCGGGTCCATGGCCACATGAAAAGCTTCCAGGGCTGGGAATATTTGGTTTCCAGGCCTGTGACCAACTATAATGAGATCACGATTCACTTCATCGAACGCATATATGTTAATTTTAAAACACCAGTCTACATTGATCACTTTCTGCAGTTGATGAGTGTTTTTTTTGGGGTAATTGGAATACAATCCAAAAATGGAACCAAAAGAGATGTCCTCTTGATGAGATATATGCATGGGTTATTTTGCAGTCTAGCAATGAAAAATCTAAAAGAGGAAAGCTTAACCTACTCAACAACTGATGATTACCACTTCAAATCAACTGCTAATGGATTAACATCTCTGTTATGGTGTACTAATTATTCAAGGCAAGTTTAG

Protein sequence

MFFSCTVTVDSTATLPTPAVDSAVSDHSGPDHPFFPAKVNAVGMMRNRAGRVTDLTLVNEAIDLNEVEGILFLCFGDGMYVRVHGHMKSFQGWEYLVSRPVTNYNEITIHFIERIYVNFKTPVYIDHFLQLMSVFFGVIGIQSKNGTKRDVLLMRYMHGLFCSLAMKNLKEESLTYSTTDDYHFKSTANGLTSLLWCTNYSRQV
Homology
[Close]
BLAST of Sed0020275 vs. NCBI nr
Match: XP_023516913.1 (replication protein A 32 kDa subunit B-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 128.3 bits (321), Expect = 7.8e-26
Identity = 90/210 (42.86%), Postives = 107/210 (50.95%), Query Frame = 0 

Query: 39  VNAVGMMRNRAGRVTDLTL--------------VNEAIDLNEVEGILFLCFGDGMYVRVH 98
           V  VGM+RNRAGR+TD+T               VNEA D NEVEGIL     DGMYVRVH
Sbjct: 72  VKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGIL-----DGMYVRVH 131

Query: 99  GHMKSFQGWEYL---VSRPVTNYNEITIHFIERIYVNF---------------------- 158
           GH+KSFQG   L     RPVT+YNEIT HFIE IYV+F                      
Sbjct: 132 GHLKSFQGKRTLNVFSIRPVTDYNEITSHFIESIYVHFYNTRLRQQSSSMTPQPQMANLS 191

Query: 159 -------KTPVYIDH-----------FLQLMSVFFGVIGIQSKNGTKRDVLLMRYMHGLF 191
                  +TP+   +             Q++  F  +    ++ G  RDV+       L 
Sbjct: 192 NTPVRGYQTPISNQYSGQAGGDGWKNLEQMVLDFLQLPSCDTERGAHRDVIAQHLKVPLE 251

[Close]
BLAST of Sed0020275 vs. NCBI nr
Match: XP_022922072.1 (replication protein A 32 kDa subunit B-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 127.9 bits (320), Expect = 1.0e-25
Identity = 90/210 (42.86%), Postives = 107/210 (50.95%), Query Frame = 0 

Query: 39  VNAVGMMRNRAGRVTDLTL--------------VNEAIDLNEVEGILFLCFGDGMYVRVH 98
           V  VGM+RNRAGR+TD+T               VNEA D NEVEGIL     DGMYVRVH
Sbjct: 72  VKLVGMVRNRAGRITDVTFALDDGTGQIDCSKWVNEAADSNEVEGIL-----DGMYVRVH 131

Query: 99  GHMKSFQGWEYL---VSRPVTNYNEITIHFIERIYVNF---------------------- 158
           GH+KSFQG   L     RPVT+YNEIT HFIE IYV+F                      
Sbjct: 132 GHLKSFQGKRTLNVFSIRPVTDYNEITSHFIESIYVHFYNTRLRQQSSSMTPQPQMANLS 191

Query: 159 -------KTPVYIDH-----------FLQLMSVFFGVIGIQSKNGTKRDVLLMRYMHGLF 191
                  +TP+   +             Q++  F  +    ++ G  RDV+       L 
Sbjct: 192 NTPVRGYQTPISNQYTGQAGGDGWKNLEQMVLDFLQLPSCDTERGAHRDVIAQHLNVPLE 251

[Close]
BLAST of Sed0020275 vs. NCBI nr
Match: XP_023516911.1 (replication protein A 32 kDa subunit B-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023516912.1 replication protein A 32 kDa subunit B-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 127.9 bits (320), Expect = 1.0e-25
Identity = 90/211 (42.65%), Postives = 107/211 (50.71%), Query Frame = 0 

Query: 39  VNAVGMMRNRAGRVTDLTL--------------VNEAIDLNEVEGILFLCFGDGMYVRVH 98
           V  VGM+RNRAGR+TD+T               VNEA D NEVEGIL     DGMYVRVH
Sbjct: 72  VKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGIL-----DGMYVRVH 131

Query: 99  GHMKSFQGWEYL---VSRPVTNYNEITIHFIERIYVNF---------------------- 158
           GH+KSFQG   L     RPVT+YNEIT HFIE IYV+F                      
Sbjct: 132 GHLKSFQGKRTLNVFSIRPVTDYNEITSHFIESIYVHFYNTRLRKQQSSSMTPQPQMANL 191

Query: 159 --------KTPVYIDH-----------FLQLMSVFFGVIGIQSKNGTKRDVLLMRYMHGL 191
                   +TP+   +             Q++  F  +    ++ G  RDV+       L
Sbjct: 192 SNTPVRGYQTPISNQYSGQAGGDGWKNLEQMVLDFLQLPSCDTERGAHRDVIAQHLKVPL 251

[Close]
BLAST of Sed0020275 vs. NCBI nr
Match: XP_008453602.1 (PREDICTED: replication protein A 32 kDa subunit B-like [Cucumis melo] >XP_008453603.1 PREDICTED: replication protein A 32 kDa subunit B-like [Cucumis melo] >XP_016901361.1 PREDICTED: replication protein A 32 kDa subunit B-like [Cucumis melo] >KAA0058271.1 replication protein A 32 kDa subunit B-like [Cucumis melo var. makuwa] >TYK11823.1 replication protein A 32 kDa subunit B-like [Cucumis melo var. makuwa])

HSP 1 Score: 127.9 bits (320), Expect = 1.0e-25
Identity = 93/211 (44.08%), Postives = 107/211 (50.71%), Query Frame = 0 

Query: 39  VNAVGMMRNRAGRVTDLTL--------------VNEAIDLNEVEGILFLCFGDGMYVRVH 98
           V  VGM+RNRAGR+TD+T               VNEA D NEVEGIL     DGMYVRVH
Sbjct: 72  VKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGIL-----DGMYVRVH 131

Query: 99  GHMKSFQGWEYL---VSRPVTNYNEITIHFIERIYVNF---------------------- 158
           GH+KSFQG   L     RPVT+YNEIT HFIE IYV+F                      
Sbjct: 132 GHLKSFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQPQMTNL 191

Query: 159 -KTPV----------YIDH--------FLQLMSVFFGVIGIQSKNGTKRDVLLMRYMHGL 191
             TPV          Y             Q++  F  +    S+ G  RDV+  +    L
Sbjct: 192 SNTPVKGYQAPIANQYTGQAGGDSWKSLEQMVLDFLQLPSCDSERGAHRDVIAQQLKVPL 251

[Close]
BLAST of Sed0020275 vs. NCBI nr
Match: KAG7023385.1 (Replication protein A 32 kDa subunit B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 127.5 bits (319), Expect = 1.3e-25
Identity = 90/211 (42.65%), Postives = 107/211 (50.71%), Query Frame = 0 

Query: 39  VNAVGMMRNRAGRVTDLTL--------------VNEAIDLNEVEGILFLCFGDGMYVRVH 98
           V  VGM+RNRAGR+TD+T               VNEA D NEVEGIL     DGMYVRVH
Sbjct: 72  VKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGIL-----DGMYVRVH 131

Query: 99  GHMKSFQGWEYL---VSRPVTNYNEITIHFIERIYVNF---------------------- 158
           GH+KSFQG   L     RPVT+YNEIT HFIE IYV+F                      
Sbjct: 132 GHLKSFQGKRTLNVFSIRPVTDYNEITSHFIESIYVHFYNTRLRKQQSSSMTPQPQMANL 191

Query: 159 --------KTPVYIDH-----------FLQLMSVFFGVIGIQSKNGTKRDVLLMRYMHGL 191
                   +TP+   +             Q++  F  +    ++ G  RDV+       L
Sbjct: 192 SNTPVRGYQTPISNQYTGQAGGDGWKNLEQMVLDFLQLPSCDTERGAHRDVIAQHLNVPL 251

[Close]
BLAST of Sed0020275 vs. ExPASy Swiss-Prot
Match: Q9ZQ19 (Replication protein A 32 kDa subunit A OS=Arabidopsis thaliana OX=3702 GN=RPA2A PE=1 SV=2)

HSP 1 Score: 57.0 bits (136), Expect = 2.9e-07
Identity = 47/182 (25.82%), Postives = 79/182 (43.41%), Query Frame = 0 

Query: 50  GRVTDLTLVNEAIDLNEVEGILFLCFGDGMYVRVHGHMKSFQGWEYLV---SRPVTNYNE 109
           GR+     V+E  D  E+E +      DG YVR+ GH+K+FQG   L+    RP+ ++NE
Sbjct: 99  GRIDCKRWVSETFDAREMESV-----RDGTYVRLSGHLKTFQGKTQLLVFSVRPIMDFNE 158

Query: 110 ITIHFIERIYVNFKTP----VYIDHFLQLMSVFF-------------GVIGIQSKNGTKR 169
           +T H+IE I+   +        +    Q ++  F              V+  Q+ +G  R
Sbjct: 159 VTFHYIECIHFYSQNSESQRQQVGDVTQSVNTTFQGGSNTNQATLLNPVVSSQNNDGNGR 218

Query: 170 ---DVLLMRYMHGLFCSL-----------------------AMKNLKEESLTYSTTDDYH 186
              D +++ Y+    C+                         +++L+ + L YST D+YH
Sbjct: 219 KNLDDMILDYLKQPACTARQQGIHIDEIAQQLKIPKNKLEGVVQSLEGDGLIYSTIDEYH 275

[Close]
BLAST of Sed0020275 vs. ExPASy Swiss-Prot
Match: Q8LFJ8 (Replication protein A 32 kDa subunit B OS=Arabidopsis thaliana OX=3702 GN=RPA2B PE=2 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 2.1e-05
Identity = 49/160 (30.63%), Postives = 65/160 (40.62%), Query Frame = 0 

Query: 78  GMYVRVHGHMKSFQG---WEYLVSRPVTNYNEITIHFIERIYVNF--------------- 137
           GMYVR+HGH+K FQG         RPVT++NEI  HF E +YV+                
Sbjct: 118 GMYVRLHGHLKIFQGKRSVNVFSVRPVTDFNEIVHHFTECMYVHMYNTKLRGGSITQDTA 177

Query: 138 ---------KTPVYIDHFLQLMSVFFGVIGIQSKNGTKRDVL--LMRYMH-----GLFCS 190
                      P     +    S  F      S +G K+ VL  L + MH     G+ C 
Sbjct: 178 TPRPQMPYSTMPTPAKPYQTGPSNQFPNQFNDSMHGVKQTVLNYLNQPMHIVSEAGVHCD 237

[Close]
BLAST of Sed0020275 vs. ExPASy Swiss-Prot
Match: Q6H7J5 (Replication protein A 32 kDa subunit B OS=Oryza sativa subsp. japonica OX=39947 GN=RPA2B PE=1 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 1.8e-04
Identity = 31/99 (31.31%), Postives = 51/99 (51.52%), Query Frame = 0 

Query: 39  VNAVGMMRNRAGRVTDLTLV--------------NEAIDLNEVEGILFLCFGDGMYVRVH 98
           V  VG M N+  RVTD++                N++ D  E+  I      +G YV V+
Sbjct: 89  VRLVGRMLNKLDRVTDVSFTLDDGTGRVPVNRWENDSTDTKEMADI-----QNGDYVIVN 148

Query: 99  GHMKSFQGWEYLVS---RPVTNYNEITIHFIERIYVNFK 121
           G +K FQG   +V+   R +TN+N++T HF+  ++V+ +
Sbjct: 149 GGLKGFQGKRQVVAYSVRRITNFNDVTHHFLHCVHVHLE 182

[Close]
BLAST of Sed0020275 vs. ExPASy Swiss-Prot
Match: Q6K9U2 (Replication protein A 32 kDa subunit A OS=Oryza sativa subsp. japonica OX=39947 GN=RPA2A PE=1 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 2.3e-04
Identity = 49/215 (22.79%), Postives = 86/215 (40.00%), Query Frame = 0 

Query: 37  AKVNAVGMMRNRAGRVTDLTL--------------VNEAIDLNEVEGILFLCFGDGMYVR 96
           A V  VG++  +  R TD++               VN+  D  E   +      +GMYV 
Sbjct: 69  ANVRLVGLVSGKTERNTDVSFTIDDGTGRLDFIRWVNDGADSAETAAV-----QNGMYVS 128

Query: 97  VHGHMKSFQGWEYLVS---RPVTNYNEITIHFIERIYVNFK------------------- 156
           V G +K  Q  +   +   RPVT+YNE+T+HFI+ + ++ +                   
Sbjct: 129 VIGSLKGLQERKRATAFAIRPVTDYNEVTLHFIQCVRMHLENTKSQIGSPAKTYSAMGSS 188

Query: 157 ---------TPVYI----------------DHFLQLMSVFFGVIGIQSKNGTKRDVLLMR 190
                    TP  +                D   ++++VF     ++S++G   D ++ R
Sbjct: 189 SSNGFSEMTTPTSVKSNPAPVLSVTNGSKTDLNTEVLNVFREPANVESEHGVHIDEIVKR 248

[Close]
BLAST of Sed0020275 vs. ExPASy TrEMBL
Match: A0A1S4DZE3 (replication protein A 32 kDa subunit B-like OS=Cucumis melo OX=3656 GN=LOC103494262 PE=3 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 4.9e-26
Identity = 93/211 (44.08%), Postives = 107/211 (50.71%), Query Frame = 0 

Query: 39  VNAVGMMRNRAGRVTDLTL--------------VNEAIDLNEVEGILFLCFGDGMYVRVH 98
           V  VGM+RNRAGR+TD+T               VNEA D NEVEGIL     DGMYVRVH
Sbjct: 72  VKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGIL-----DGMYVRVH 131

Query: 99  GHMKSFQGWEYL---VSRPVTNYNEITIHFIERIYVNF---------------------- 158
           GH+KSFQG   L     RPVT+YNEIT HFIE IYV+F                      
Sbjct: 132 GHLKSFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQPQMTNL 191

Query: 159 -KTPV----------YIDH--------FLQLMSVFFGVIGIQSKNGTKRDVLLMRYMHGL 191
             TPV          Y             Q++  F  +    S+ G  RDV+  +    L
Sbjct: 192 SNTPVKGYQAPIANQYTGQAGGDSWKSLEQMVLDFLQLPSCDSERGAHRDVIAQQLKVPL 251

[Close]
BLAST of Sed0020275 vs. ExPASy TrEMBL
Match: A0A5D3CIS3 (Replication protein A 32 kDa subunit B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold152G00360 PE=3 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 4.9e-26
Identity = 93/211 (44.08%), Postives = 107/211 (50.71%), Query Frame = 0 

Query: 39  VNAVGMMRNRAGRVTDLTL--------------VNEAIDLNEVEGILFLCFGDGMYVRVH 98
           V  VGM+RNRAGR+TD+T               VNEA D NEVEGIL     DGMYVRVH
Sbjct: 72  VKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGIL-----DGMYVRVH 131

Query: 99  GHMKSFQGWEYL---VSRPVTNYNEITIHFIERIYVNF---------------------- 158
           GH+KSFQG   L     RPVT+YNEIT HFIE IYV+F                      
Sbjct: 132 GHLKSFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQPQMTNL 191

Query: 159 -KTPV----------YIDH--------FLQLMSVFFGVIGIQSKNGTKRDVLLMRYMHGL 191
             TPV          Y             Q++  F  +    S+ G  RDV+  +    L
Sbjct: 192 SNTPVKGYQAPIANQYTGQAGGDSWKSLEQMVLDFLQLPSCDSERGAHRDVIAQQLKVPL 251

[Close]
BLAST of Sed0020275 vs. ExPASy TrEMBL
Match: A0A6J1E235 (replication protein A 32 kDa subunit B-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430133 PE=3 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 4.9e-26
Identity = 90/210 (42.86%), Postives = 107/210 (50.95%), Query Frame = 0 

Query: 39  VNAVGMMRNRAGRVTDLTL--------------VNEAIDLNEVEGILFLCFGDGMYVRVH 98
           V  VGM+RNRAGR+TD+T               VNEA D NEVEGIL     DGMYVRVH
Sbjct: 72  VKLVGMVRNRAGRITDVTFALDDGTGQIDCSKWVNEAADSNEVEGIL-----DGMYVRVH 131

Query: 99  GHMKSFQGWEYL---VSRPVTNYNEITIHFIERIYVNF---------------------- 158
           GH+KSFQG   L     RPVT+YNEIT HFIE IYV+F                      
Sbjct: 132 GHLKSFQGKRTLNVFSIRPVTDYNEITSHFIESIYVHFYNTRLRQQSSSMTPQPQMANLS 191

Query: 159 -------KTPVYIDH-----------FLQLMSVFFGVIGIQSKNGTKRDVLLMRYMHGLF 191
                  +TP+   +             Q++  F  +    ++ G  RDV+       L 
Sbjct: 192 NTPVRGYQTPISNQYTGQAGGDGWKNLEQMVLDFLQLPSCDTERGAHRDVIAQHLNVPLE 251

[Close]
BLAST of Sed0020275 vs. ExPASy TrEMBL
Match: A0A6J1E347 (replication protein A 32 kDa subunit B-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430133 PE=3 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 6.4e-26
Identity = 90/211 (42.65%), Postives = 107/211 (50.71%), Query Frame = 0 

Query: 39  VNAVGMMRNRAGRVTDLTL--------------VNEAIDLNEVEGILFLCFGDGMYVRVH 98
           V  VGM+RNRAGR+TD+T               VNEA D NEVEGIL     DGMYVRVH
Sbjct: 72  VKLVGMVRNRAGRITDVTFALDDGTGQIDCSKWVNEAADSNEVEGIL-----DGMYVRVH 131

Query: 99  GHMKSFQGWEYL---VSRPVTNYNEITIHFIERIYVNF---------------------- 158
           GH+KSFQG   L     RPVT+YNEIT HFIE IYV+F                      
Sbjct: 132 GHLKSFQGKRTLNVFSIRPVTDYNEITSHFIESIYVHFYNTRLRKQQSSSMTPQPQMANL 191

Query: 159 --------KTPVYIDH-----------FLQLMSVFFGVIGIQSKNGTKRDVLLMRYMHGL 191
                   +TP+   +             Q++  F  +    ++ G  RDV+       L
Sbjct: 192 SNTPVRGYQTPISNQYTGQAGGDGWKNLEQMVLDFLQLPSCDTERGAHRDVIAQHLNVPL 251

[Close]
BLAST of Sed0020275 vs. ExPASy TrEMBL
Match: A0A6J1JJ51 (replication protein A 32 kDa subunit B OS=Cucurbita maxima OX=3661 GN=LOC111485578 PE=3 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 8.4e-26
Identity = 91/211 (43.13%), Postives = 106/211 (50.24%), Query Frame = 0 

Query: 39  VNAVGMMRNRAGRVTDLTL--------------VNEAIDLNEVEGILFLCFGDGMYVRVH 98
           V  VGM+RNRAGR+TD+T               VNEA D NEVEGIL     DGMYVRVH
Sbjct: 72  VKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGIL-----DGMYVRVH 131

Query: 99  GHMKSFQGWEYL---VSRPVTNYNEITIHFIERIYVNF---------------------- 158
           GH+KSFQG   L     RPVT+YNEIT HFIE IYV+F                      
Sbjct: 132 GHLKSFQGKRTLNVFSIRPVTDYNEITSHFIESIYVHFYNTRLRKQQSSSMTPQPQMANL 191

Query: 159 -KTPVY------------------IDHFLQLMSVFFGVIGIQSKNGTKRDVLLMRYMHGL 191
             TPV                     +  Q++  F  +    ++ G  RDV+       L
Sbjct: 192 SNTPVRGYQTPMSNQYTGQAGGDGWKNLEQMVLDFLQLPSCDTERGAHRDVIAQHLKVPL 251

[Close]
BLAST of Sed0020275 vs. TAIR 10
Match: AT2G24490.1 (replicon protein A2 )

HSP 1 Score: 57.0 bits (136), Expect = 2.1e-08
Identity = 47/182 (25.82%), Postives = 79/182 (43.41%), Query Frame = 0 

Query: 50  GRVTDLTLVNEAIDLNEVEGILFLCFGDGMYVRVHGHMKSFQGWEYLV---SRPVTNYNE 109
           GR+     V+E  D  E+E +      DG YVR+ GH+K+FQG   L+    RP+ ++NE
Sbjct: 99  GRIDCKRWVSETFDAREMESV-----RDGTYVRLSGHLKTFQGKTQLLVFSVRPIMDFNE 158

Query: 110 ITIHFIERIYVNFKTP----VYIDHFLQLMSVFF-------------GVIGIQSKNGTKR 169
           +T H+IE I+   +        +    Q ++  F              V+  Q+ +G  R
Sbjct: 159 VTFHYIECIHFYSQNSESQRQQVGDVTQSVNTTFQGGSNTNQATLLNPVVSSQNNDGNGR 218

Query: 170 ---DVLLMRYMHGLFCSL-----------------------AMKNLKEESLTYSTTDDYH 186
              D +++ Y+    C+                         +++L+ + L YST D+YH
Sbjct: 219 KNLDDMILDYLKQPACTARQQGIHIDEIAQQLKIPKNKLEGVVQSLEGDGLIYSTIDEYH 275

[Close]
BLAST of Sed0020275 vs. TAIR 10
Match: AT2G24490.2 (replicon protein A2 )

HSP 1 Score: 57.0 bits (136), Expect = 2.1e-08
Identity = 47/182 (25.82%), Postives = 79/182 (43.41%), Query Frame = 0 

Query: 50  GRVTDLTLVNEAIDLNEVEGILFLCFGDGMYVRVHGHMKSFQGWEYLV---SRPVTNYNE 109
           GR+     V+E  D  E+E +      DG YVR+ GH+K+FQG   L+    RP+ ++NE
Sbjct: 99  GRIDCKRWVSETFDAREMESV-----RDGTYVRLSGHLKTFQGKTQLLVFSVRPIMDFNE 158

Query: 110 ITIHFIERIYVNFKTP----VYIDHFLQLMSVFF-------------GVIGIQSKNGTKR 169
           +T H+IE I+   +        +    Q ++  F              V+  Q+ +G  R
Sbjct: 159 VTFHYIECIHFYSQNSESQRQQVGDVTQSVNTTFQGGSNTNQATLLNPVVSSQNNDGNGR 218

Query: 170 ---DVLLMRYMHGLFCSL-----------------------AMKNLKEESLTYSTTDDYH 186
              D +++ Y+    C+                         +++L+ + L YST D+YH
Sbjct: 219 KNLDDMILDYLKQPACTARQQGIHIDEIAQQLKIPKNKLEGVVQSLEGDGLIYSTIDEYH 275

[Close]
BLAST of Sed0020275 vs. TAIR 10
Match: AT3G02920.1 (Replication protein A, subunit RPA32 )

HSP 1 Score: 50.8 bits (120), Expect = 1.5e-06
Identity = 49/160 (30.63%), Postives = 65/160 (40.62%), Query Frame = 0 

Query: 78  GMYVRVHGHMKSFQG---WEYLVSRPVTNYNEITIHFIERIYVNF--------------- 137
           GMYVR+HGH+K FQG         RPVT++NEI  HF E +YV+                
Sbjct: 118 GMYVRLHGHLKIFQGKRSVNVFSVRPVTDFNEIVHHFTECMYVHMYNTKLRGGSITQDTA 177

Query: 138 ---------KTPVYIDHFLQLMSVFFGVIGIQSKNGTKRDVL--LMRYMH-----GLFCS 190
                      P     +    S  F      S +G K+ VL  L + MH     G+ C 
Sbjct: 178 TPRPQMPYSTMPTPAKPYQTGPSNQFPNQFNDSMHGVKQTVLNYLNQPMHIVSEAGVHCD 237

[Close]
BLAST of Sed0020275 vs. TAIR 10
Match: AT3G02920.2 (Replication protein A, subunit RPA32 )

HSP 1 Score: 50.8 bits (120), Expect = 1.5e-06
Identity = 49/160 (30.63%), Postives = 65/160 (40.62%), Query Frame = 0 

Query: 78  GMYVRVHGHMKSFQG---WEYLVSRPVTNYNEITIHFIERIYVNF--------------- 137
           GMYVR+HGH+K FQG         RPVT++NEI  HF E +YV+                
Sbjct: 138 GMYVRLHGHLKIFQGKRSVNVFSVRPVTDFNEIVHHFTECMYVHMYNTKLRGGSITQDTA 197

Query: 138 ---------KTPVYIDHFLQLMSVFFGVIGIQSKNGTKRDVL--LMRYMH-----GLFCS 190
                      P     +    S  F      S +G K+ VL  L + MH     G+ C 
Sbjct: 198 TPRPQMPYSTMPTPAKPYQTGPSNQFPNQFNDSMHGVKQTVLNYLNQPMHIVSEAGVHCD 257

The following BLAST results are available for this feature:
BLAST of Sed0020275 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_023516913.17.8e-2642.86replication protein A 32 kDa subunit B-like isoform X2 [Cucurbita pepo subsp. pe... [more]
XP_022922072.11.0e-2542.86replication protein A 32 kDa subunit B-like isoform X2 [Cucurbita moschata][more]
XP_023516911.11.0e-2542.65replication protein A 32 kDa subunit B-like isoform X1 [Cucurbita pepo subsp. pe... [more]
XP_008453602.11.0e-2544.08PREDICTED: replication protein A 32 kDa subunit B-like [Cucumis melo] >XP_008453... [more]
KAG7023385.11.3e-2542.65Replication protein A 32 kDa subunit B [Cucurbita argyrosperma subsp. argyrosper... [more]
BLAST of Sed0020275 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9ZQ192.9e-0725.82Replication protein A 32 kDa subunit A OS=Arabidopsis thaliana OX=3702 GN=RPA2A ... [more]
Q8LFJ82.1e-0530.63Replication protein A 32 kDa subunit B OS=Arabidopsis thaliana OX=3702 GN=RPA2B ... [more]
Q6H7J51.8e-0431.31Replication protein A 32 kDa subunit B OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q6K9U22.3e-0422.79Replication protein A 32 kDa subunit A OS=Oryza sativa subsp. japonica OX=39947 ... [more]
BLAST of Sed0020275 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A1S4DZE34.9e-2644.08replication protein A 32 kDa subunit B-like OS=Cucumis melo OX=3656 GN=LOC103494... [more]
A0A5D3CIS34.9e-2644.08Replication protein A 32 kDa subunit B-like OS=Cucumis melo var. makuwa OX=11946... [more]
A0A6J1E2354.9e-2642.86replication protein A 32 kDa subunit B-like isoform X2 OS=Cucurbita moschata OX=... [more]
A0A6J1E3476.4e-2642.65replication protein A 32 kDa subunit B-like isoform X1 OS=Cucurbita moschata OX=... [more]
A0A6J1JJ518.4e-2643.13replication protein A 32 kDa subunit B OS=Cucurbita maxima OX=3661 GN=LOC1114855... [more]
BLAST of Sed0020275 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G24490.12.1e-0825.82replicon protein A2 [more]
AT2G24490.22.1e-0825.82replicon protein A2 [more]
AT3G02920.11.5e-0630.63Replication protein A, subunit RPA32 [more]
AT3G02920.21.5e-0630.63Replication protein A, subunit RPA32 [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D2.40.50.140coord: 38..121
e-value: 4.0E-7
score: 31.7
NoneNo IPR availablePANTHERPTHR13989:SF42REPLICATION PROTEIN A 32 KDA SUBUNIT A-LIKEcoord: 47..118
IPR040260Replication factor A protein-likePANTHERPTHR13989REPLICATION PROTEIN A-RELATEDcoord: 47..118
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 75..118

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0020275.1Sed0020275.1mRNA