Pay0021495 (gene) Melon (Payzawat) v1

BLAST of Pay0021495 vs. ExPASy Swiss-Prot
Match: Q9M0S5 (Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2)

HSP 1 Score: 1124.0 bits (2906), Expect = 0.0e+00
Identity = 525/711 (73.84%), Postives = 596/711 (83.83%), Query Frame = 0

Query: 58  TVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHATSVT 117
           T+    RR  +RV+E+EA    +T  + K+  G+  PLGVS+VD GINFA+FSQ+ATSVT
Sbjct: 57  TLKVTCRRAHERVVEEEASTMTET-KLFKVSSGEVSPLGVSQVDKGINFALFSQNATSVT 116

Query: 118 ICLSL-----DGRIDDGMLELKLDPDENRTGDIWHICIQDLQRKNVLYGYRIDGPQGWHH 177
           +CLSL     D   DDGM+EL LDP  N+TGD WHIC++DL   NVLYGYR+DGP  W  
Sbjct: 117 LCLSLSQSGKDDTDDDGMIELVLDPSVNKTGDTWHICVEDLPLNNVLYGYRVDGPGEWQQ 176

Query: 178 GHRYDDGTMLLDPYAKFVEGRRFFG-GENKSSGFLGTYDFESLPFDWGNDYKLPNIPEKD 237
           GHR+D   +LLDPYAK V+G   FG    K + F GTYDFES PFDWG+DYK PNIPEKD
Sbjct: 177 GHRFDRSILLLDPYAKLVKGHSSFGDSSQKFAQFYGTYDFESSPFDWGDDYKFPNIPEKD 236

Query: 238 LVIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQR 297
           LVIYEMNVR FTADESSG+  +  GSYLG IEKIPHL +LG+NAVELLPVFEFDELE QR
Sbjct: 237 LVIYEMNVRAFTADESSGMDPAIGGSYLGFIEKIPHLQDLGINAVELLPVFEFDELELQR 296

Query: 298 HPNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVY 357
             NPRDHM+NTWGYST+NFFAPMSRYAS  G P+ AS+EFK+MVKALH+AGIEVILDVVY
Sbjct: 297 RSNPRDHMVNTWGYSTVNFFAPMSRYASGEGDPIKASKEFKEMVKALHSAGIEVILDVVY 356

Query: 358 NHTNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESL 417
           NHTNE DD  PYTTSFRGIDNKVYYMLD   N Q  NFSGCGNTLNCNHPVVMELIL+SL
Sbjct: 357 NHTNEADDKYPYTTSFRGIDNKVYYMLD--PNNQLLNFSGCGNTLNCNHPVVMELILDSL 416

Query: 418 RHWVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLY 477
           RHWV EYHVDGFRFDLASVLCR TDG+PLSAPPLIRAI+KD++LSRCKIIAEPWDCGGLY
Sbjct: 417 RHWVTEYHVDGFRFDLASVLCRATDGSPLSAPPLIRAIAKDSVLSRCKIIAEPWDCGGLY 476

Query: 478 LVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYNVNKRKPYHGINF 537
           LVG+FPNWDRWAEWNG+YRDD+R+FIKGD GMKGSFATRV+GSSDLY VN+RKPYHG+NF
Sbjct: 477 LVGKFPNWDRWAEWNGMYRDDVRRFIKGDSGMKGSFATRVSGSSDLYQVNQRKPYHGVNF 536

Query: 538 VIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRSRQMKN 597
           VIAHDGFTLRDLVSYN KHN+ANGEGGNDGCNDN SWNCGFEGET D  IK+LR+RQMKN
Sbjct: 537 VIAHDGFTLRDLVSYNFKHNEANGEGGNDGCNDNHSWNCGFEGETGDAHIKSLRTRQMKN 596

Query: 598 FHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVI 657
           FHLALM SQGTPMMLMGDEYGHTRYGNNNSYGHD +LN+F W++L+A+K++HFRFFSEVI
Sbjct: 597 FHLALMISQGTPMMLMGDEYGHTRYGNNNSYGHDTSLNNFQWKELDAKKQNHFRFFSEVI 656

Query: 658 KFRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDD-NGEDVYLAFNAQDYFV 717
           KFR  H H+   ENFL + +ITWHE NWDN ESKFLA+TLHD   G D+Y+AFNA DYFV
Sbjct: 657 KFRHSH-HVLKHENFLTQGEITWHEDNWDNSESKFLAFTLHDGIGGRDIYVAFNAHDYFV 716

Query: 718 NVSLPSPPTNRRWFRVVDTNLESPHDFVLDGLPGVGSSYNLAPYSSILLKA 762
              +P PP  ++WFRV DTNLESP DFV +G+ GV  +YN+AP+SSILL++
Sbjct: 717 KALIPQPPPGKQWFRVADTNLESPDDFVREGVAGVADTYNVAPFSSILLQS 763

BLAST of Pay0021495 vs. ExPASy Swiss-Prot
Match: B9G434 (Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=2 SV=1)

HSP 1 Score: 1100.9 bits (2846), Expect = 0.0e+00
Identity = 510/689 (74.02%), Postives = 582/689 (84.47%), Query Frame = 0

Query: 80  DTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHATSVTICLSLDGRIDD-----GMLELK 139
           DT    K   G+AFPLGVS+V+ G+NFA+FSQHA+SV +CL L GR  +      ++E  
Sbjct: 93  DTEMPFKYSSGKAFPLGVSQVEGGLNFALFSQHASSVILCLKLPGRGTEDEKGADVVEFV 152

Query: 140 LDPDENRTGDIWHICIQDLQRKNVLYGYRIDGPQGWHHGHRYDDGTMLLDPYAKFVEGRR 199
           LD  +N+TGDIWH+ ++ L    VLYGYR+ GPQGW  GHR+D  T+LLDPYAK V GR+
Sbjct: 153 LDQQKNKTGDIWHVIVEGLPASGVLYGYRVGGPQGWDQGHRFDSSTVLLDPYAKLVSGRK 212

Query: 200 FFG-GENKSSGFLGTYDFESLPFDWGNDYKLPNIPEKDLVIYEMNVRGFTADESSGLPSS 259
           +FG  E KSS   GTYDF+S PFDWG+DY+LPN+PE DLVIYEMNVR FTADESSGL S+
Sbjct: 213 YFGVAEEKSSQHFGTYDFDSSPFDWGDDYRLPNLPEADLVIYEMNVRAFTADESSGLDST 272

Query: 260 TRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPRDHMINTWGYSTINFFAP 319
           +RGSYLG+I+KIPHLLELGVNAVELLPVFE+DELEF+R+PNPRDHM+NTWGYSTINFFAP
Sbjct: 273 SRGSYLGLIDKIPHLLELGVNAVELLPVFEYDELEFKRYPNPRDHMVNTWGYSTINFFAP 332

Query: 320 MSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYNHTNEGDDVNPYTTSFRGIDNK 379
           MSRYASAGGGP+ AS+E KQMVK LH AGIEVILDVVYNHTNE DD +PY TSFRGIDNK
Sbjct: 333 MSRYASAGGGPVAASKELKQMVKELHKAGIEVILDVVYNHTNEADDAHPYMTSFRGIDNK 392

Query: 380 VYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRHWVVEYHVDGFRFDLASVLCR 439
           VYYMLDL  N +  NFSGCGNTLNCNHPVV ELIL+SLRHWV EYH+DGFRFDLASVLCR
Sbjct: 393 VYYMLDLNKNAELLNFSGCGNTLNCNHPVVKELILDSLRHWVEEYHIDGFRFDLASVLCR 452

Query: 440 GTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDDI 499
           G DG PL APPLI+ I+KDA+LSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNG YRDD+
Sbjct: 453 GPDGCPLDAPPLIKEIAKDAVLSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGKYRDDL 512

Query: 500 RKFIKGDCGMKGSFATRVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDA 559
           R+FIKGD GMKG FATRV+GS+DLY VN+RKPYHG+NFVIAHDGFTL DLVSYN+KHNDA
Sbjct: 513 RRFIKGDPGMKGVFATRVSGSADLYQVNERKPYHGVNFVIAHDGFTLCDLVSYNLKHNDA 572

Query: 560 NGEGGNDGCNDNFSWNCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGH 619
           NGEGG DGCNDNFSWNCG EGET D ++ +LRSRQMKNFH+ALM SQGTPMMLMGDEYGH
Sbjct: 573 NGEGGCDGCNDNFSWNCGVEGETNDLNVLSLRSRQMKNFHVALMISQGTPMMLMGDEYGH 632

Query: 620 TRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLNKNDIT 679
           TRYGNNNSYGHD  +N+F WEQLE R+  HFRFFSE+IKFR  +P +  R+ FLNKND+T
Sbjct: 633 TRYGNNNSYGHDTCINNFQWEQLEQRRDGHFRFFSEMIKFRHSNP-ILRRDRFLNKNDVT 692

Query: 680 WHESNWDNPESKFLAYTLHDDN-GEDVYLAFNAQDYFVNVSLPSPPTNRRWFRVVDTNLE 739
           WHE  W+N ESKFLA+T+HD N G D+YLAFNA DYFV+  +P PP ++ W RVVDTNLE
Sbjct: 693 WHEDCWENQESKFLAFTVHDHNSGGDIYLAFNAHDYFVDAVIPPPPHHKCWNRVVDTNLE 752

Query: 740 SPHDFVLDGLPGVGSSYNLAPYSSILLKA 762
           SP+D V +G+P  G  Y +APYSSILLKA
Sbjct: 753 SPNDIVPEGVPFTGPKYRIAPYSSILLKA 780

BLAST of Pay0021495 vs. ExPASy Swiss-Prot
Match: O04196 (Isoamylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 620.5 bits (1599), Expect = 2.5e-176
Identity = 348/749 (46.46%), Postives = 450/749 (60.08%), Query Frame = 0

Query: 58  TVSAFSRR--DQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHATS 117
           ++SA  RR  + + +   E P   D      I  G   P G +  D+G+NF+++S ++ S
Sbjct: 41  SISAKDRRSNEAENIAVVEKPLKSD---RFFISDGLPSPFGPTVRDDGVNFSVYSTNSVS 100

Query: 118 VTICL-SL-DGRIDDGMLELKLDPDENRTGDIWHICIQDLQRKNVLYGYRIDGPQGWHHG 177
            TICL SL D R +    E++LDP  NRTG +WH+ ++    K++LYGYR DG      G
Sbjct: 101 ATICLISLSDLRQNKVTEEIQLDPSRNRTGHVWHVFLRG-DFKDMLYGYRFDGKFSPEEG 160

Query: 178 HRYDDGTMLLDPYAKFVEGRRFFG----GENKSSGFLGTYDFESLPFDWGNDYKLPNIPE 237
           H YD   +LLDPYAK +  R  FG     +N               FDW  D  L  +P+
Sbjct: 161 HYYDSSNILLDPYAKAIISRDEFGVLGPDDNCWPQMACMVPTREEEFDWEGDMHL-KLPQ 220

Query: 238 KDLVIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEF 297
           KDLVIYEM+VRGFT  ESS +     G+Y GV EK+ HL ELG+N +EL+P  EF+ELE+
Sbjct: 221 KDLVIYEMHVRGFTRHESSKI--EFPGTYQGVAEKLDHLKELGINCIELMPCHEFNELEY 280

Query: 298 QRHPN-PRDHMINTWGYSTINFFAPMSRYASAGGGPL--NASQEFKQMVKALHAAGIEVI 357
             +     DH +N WGYSTI FF+PM RYASA        A  EFK +VK  H  GIEVI
Sbjct: 281 YSYNTILGDHRVNFWGYSTIGFFSPMIRYASASSNNFAGRAINEFKILVKEAHKRGIEVI 340

Query: 358 LDVVYNHTNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMEL 417
           +DVV NHT EG++  P   SFRG+DN VYYML  K  G+++N+SGCGNT NCNHPVV + 
Sbjct: 341 MDVVLNHTAEGNEKGP-IFSFRGVDNSVYYMLAPK--GEFYNYSGCGNTFNCNHPVVRQF 400

Query: 418 ILESLRHWVVEYHVDGFRFDLASVLCRG-------------------TDGAPLSAPPLIR 477
           IL+ LR+WV E HVDGFRFDL S++ R                    T G P+S PP+I 
Sbjct: 401 ILDCLRYWVTEMHVDGFRFDLGSIMSRSSSLWDAANVYGADVEGDLLTTGTPISCPPVID 460

Query: 478 AISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSF 537
            IS D IL   K+IAE WD GGLY VG FP+W  W+EWNG +RD +R+FIKG  G  G+F
Sbjct: 461 MISNDPILRGVKLIAEAWDAGGLYQVGMFPHWGIWSEWNGKFRDVVRQFIKGTDGFSGAF 520

Query: 538 ATRVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFS 597
           A  + GS +LY    RKP+H INF+ AHDGFTL DLV+YN K+N ANGE  NDG N N+S
Sbjct: 521 AECLCGSPNLYQ-GGRKPWHSINFICAHDGFTLADLVTYNNKNNLANGEENNDGENHNYS 580

Query: 598 WNCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNA 657
           WNCG EG+    S+K LR RQM+NF ++LM SQG PM+ MGDEYGHT+ GNNN+Y HDN 
Sbjct: 581 WNCGEEGDFASISVKRLRKRQMRNFFVSLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNY 640

Query: 658 LNHFLWEQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLNKNDITWHE-----SNWDNP 717
           +N+F W++ E    D FRF   +IKFR +   L    +F     + WH       NW + 
Sbjct: 641 MNYFRWDKKEEAHSDFFRFCRILIKFRDECESL-GLNDFPTAKRLQWHGLAPEIPNW-SE 700

Query: 718 ESKFLAYTLHDDNGEDVYLAFNAQDYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGL 760
            S+F+A++L D   +++Y+AFN       VSLP+ P   RW   VDT+  SP+D +   L
Sbjct: 701 TSRFVAFSLVDSVKKEIYVAFNTSHLATLVSLPNRP-GYRWEPFVDTSKPSPYDCITPDL 760

BLAST of Pay0021495 vs. ExPASy Swiss-Prot
Match: D0TZF0 (Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 615.1 bits (1585), Expect = 1.0e-174
Identity = 337/738 (45.66%), Postives = 440/738 (59.62%), Query Frame = 0

Query: 66  DQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHATSVTICLSL--D 125
           +++ V     PE    G   ++  G   PLG + +D G+NFA++S  A++ ++CL    D
Sbjct: 75  EEEEVEAVVMPERYALGGACRVLAGMPAPLGATALDGGVNFAVYSAGASAASLCLFTPDD 134

Query: 126 GRIDDGMLELKLDPDENRTGDIWHICIQDLQRKNVLYGYRIDGPQGWHHGHRYDDGTMLL 185
              D+   E+ LDP  NRTG++WH+ I+  +  N+LYGYR DG    H G  +D   +++
Sbjct: 135 LEADEVTEEVPLDPLFNRTGNVWHVFIEG-ELHNMLYGYRFDGMFAPHCGQYFDVSNVVV 194

Query: 186 DPYAKFVEGRRFFG----GENKSSGFLGTYDFESLPFDWGNDYKLPNIPEKDLVIYEMNV 245
           DPYAK V  R  +G    G +      G        FDW  D  L   P+KDLVIYEM++
Sbjct: 195 DPYAKAVISRGEYGVPGPGGDCWPQMAGMIPLPYSTFDWQGDLPL-RYPQKDLVIYEMHL 254

Query: 246 RGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPRDHM 305
           RGFT   SS +     G+Y+G I K+ +L ELGVN VEL+P  EF+ELE+    +     
Sbjct: 255 RGFTKHSSSNVEHP--GTYIGAISKLDYLKELGVNCVELMPCHEFNELEYFSCSS----K 314

Query: 306 INTWGYSTINFFAPMSRYASAG--GGPLNASQEFKQMVKALHAAGIEVILDVVYNHTNEG 365
           +N WGYSTINFF+PM RY+S G      +A  EFK  V+  H  GIEVI+DVV+NHT EG
Sbjct: 315 MNFWGYSTINFFSPMIRYSSGGIRNCGRDAINEFKTFVREAHKRGIEVIMDVVFNHTAEG 374

Query: 366 DDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRHWVVE 425
           ++  P   SFRGIDN  YYML  K  G+++N+SGCGNT NCNHPVV E I++ LR+WV E
Sbjct: 375 NEKGP-ILSFRGIDNSTYYMLAPK--GEFYNYSGCGNTFNCNHPVVREFIVDCLRYWVTE 434

Query: 426 YHVDGFRFDLASVLCRG-------------------TDGAPLSAPPLIRAISKDAILSRC 485
            HVDGFRFDLAS++ RG                   T G PL+ PPLI  IS D IL   
Sbjct: 435 MHVDGFRFDLASIMTRGCSLWDPVNVYGSPVEGDMTTTGTPLATPPLIDMISNDPILGDV 494

Query: 486 KIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLY 545
           K+IAE WD GGLY VG+FP+W  W+EWNG YRD +R+FIKG  G  G FA  + GS  LY
Sbjct: 495 KLIAEAWDAGGLYQVGQFPHWKIWSEWNGKYRDIVRQFIKGTDGFAGGFAECLCGSPHLY 554

Query: 546 NVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETED 605
               RKP+H INFV AHDGFTL DLV+YN K+N +NGE   DG N N SWNCG EGE   
Sbjct: 555 QAGGRKPWHSINFVCAHDGFTLADLVTYNKKYNSSNGEDNRDGENHNLSWNCGEEGEFAG 614

Query: 606 TSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEA 665
            S+K LR RQM+NF ++LM SQG PM  MGDEYGHT+ GNNN+Y HD+ +N+F W++ E 
Sbjct: 615 LSVKRLRKRQMRNFFVSLMVSQGVPMFYMGDEYGHTKGGNNNTYCHDHYVNYFRWDKKE- 674

Query: 666 RKRDHFRFFSEVIKFRRKHPHLFSRENFLNKNDITWHESNWDNPE----SKFLAYTLHDD 725
              D  RF S + KFR++   L    +F     + WH      P+    S+F+A++  D+
Sbjct: 675 ESSDLQRFCSLMTKFRKQCESL-GLADFPTAQRLHWHGHQPGKPDWSETSRFVAFSTKDE 734

Query: 726 NGEDVYLAFNAQDYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGLPGVGSS------ 761
              ++Y+AFNA      V LP  P   RW  +VDT   +P+DF+ D LP    +      
Sbjct: 735 TKGEIYVAFNASHLPAVVGLPERP-GYRWEPLVDTGKPAPYDFLTDDLPDRAHAVHLFSH 794

BLAST of Pay0021495 vs. ExPASy Swiss-Prot
Match: P0A4Y5 (Glycogen operon protein GlgX homolog OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=glgX PE=3 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 2.4e-131
Identity = 302/708 (42.66%), Postives = 389/708 (54.94%), Query Frame = 0

Query: 75  APENLDTGPML-KIFPGQAFPLGVSEVDNGINFAIFSQHATSVTICLSLDGRIDDGMLEL 134
           A E+  TGP L  ++PG A+PLG +    G NF++FS+ A  V +CL     ID+  +E 
Sbjct: 6   AGESDGTGPALPTVWPGNAYPLGATYDGAGTNFSLFSEIAEKVELCL-----IDEDGVES 65

Query: 135 KLDPDENRTGDIWHICIQDLQRKNVLYGYRIDGPQGWHHGHRYDDGTMLLDPYAKFVEGR 194
           ++  DE   G +WH  + ++      YG+R+ GP     GHR D   +LLDPY K   G 
Sbjct: 66  RIPLDE-VDGYVWHAYLPNI-TPGQRYGFRVHGPFDPAAGHRCDPSKLLLDPYGKSFHG- 125

Query: 195 RFFGGENKSSGFLGTYDFESLP------------------FDWGNDYKLPNIPEKDLVIY 254
            F  G+   S  +   D +S P                  FDW  D + P  P  + VIY
Sbjct: 126 DFTFGQALYSYDVNAVDPDSTPPMVDSLGHTMTSVVINPFFDWAYD-RSPRTPYHETVIY 185

Query: 255 EMNVRGFTADESSGLPSSTRGSYLGVIEK--IPHLLELGVNAVELLPVFEFDELEFQRHP 314
           E +V+G T    S +P   RG+Y G+     I HL EL V AVEL+PV +F  L   R  
Sbjct: 186 EAHVKGMTQTHPS-IPPELRGTYAGLAHPVIIDHLNELNVTAVELMPVHQF--LHDSRLL 245

Query: 315 NPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYNH 374
           +    + N WGY+T  FFAP  +YAS      +A  EFK MV++LH AGIEVILDVVYNH
Sbjct: 246 DL--GLRNYWGYNTFGFFAPHHQYASTRQAG-SAVAEFKTMVRSLHEAGIEVILDVVYNH 305

Query: 375 TNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRH 434
           T EG+ + P T +FRGIDN  YY L   +   Y +F+G GN+LN  HP  ++LI++SLR+
Sbjct: 306 TAEGNHLGP-TINFRGIDNTAYYRLMDHDLRFYKDFTGTGNSLNARHPHTLQLIMDSLRY 365

Query: 435 WVVEYHVDGFRFDLASVLCRGT-DGAPLSAPPLIRAISKDAILSRCKIIAEPWDCG-GLY 494
           WV+E HVDGFRFDLAS L R   D   LSA      + +D ++S+ K+IAEPWD G G Y
Sbjct: 366 WVIEMHVDGFRFDLASTLARELHDVDRLSA--FFDLVQQDPVVSQVKLIAEPWDVGEGGY 425

Query: 495 LVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYNVNKRKPYHGINF 554
            VG FP    W EWNG YRD +R + +G+    G FA+R+ GSSDLY    R+P   INF
Sbjct: 426 QVGNFPG--LWTEWNGKYRDTVRDYWRGEPATLGEFASRLTGSSDLYEATGRRPSASINF 485

Query: 555 VIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRSRQMKN 614
           V AHDGFTL DLVSYN KHN+ANGE   DG + N SWNCG EG T+D  I ALR+RQM+N
Sbjct: 486 VTAHDGFTLNDLVSYNDKHNEANGENNRDGESYNRSWNCGVEGPTDDPDILALRARQMRN 545

Query: 615 FHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVI 674
               LM SQGTPM+  GDE G T+YGNNN Y  D+ L+   W  ++ +  D   F  +  
Sbjct: 546 MWATLMVSQGTPMIAHGDEIGRTQYGNNNVYCQDSELSWMDWSLVD-KNADLLAFARKAT 605

Query: 675 KFRRKHPHLFSRENFL---------NKNDITW---------HES---NWDNPESKFL--- 731
             R+ H  +F R  F             DI W         HE     +D   + FL   
Sbjct: 606 TLRKNH-KVFRRRRFFEGEPIRSGDEVRDIAWLTPSGREMTHEDWGRGFDRCVAVFLNGE 665

BLAST of Pay0021495 vs. ExPASy TrEMBL
Match: A0A1S3CS22 (isoamylase 3, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503684 PE=4 SV=1)

HSP 1 Score: 1512.3 bits (3914), Expect = 0.0e+00
Identity = 711/716 (99.30%), Postives = 714/716 (99.72%), Query Frame = 0

Query: 48  KTKQGGVKLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFA 107
           +TKQGGVKLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFA
Sbjct: 54  ETKQGGVKLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFA 113

Query: 108 IFSQHATSVTICLSLDGRIDDGMLELKLDPDENRTGDIWHICIQDLQRKNVLYGYRIDGP 167
           IFSQHATSVTICLSLDGRIDDGMLE KLDPD+NRTGDIWHICIQDLQRKNVLYGYRIDGP
Sbjct: 114 IFSQHATSVTICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLQRKNVLYGYRIDGP 173

Query: 168 QGWHHGHRYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNI 227
           QGWHHGHRYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNI
Sbjct: 174 QGWHHGHRYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNI 233

Query: 228 PEKDLVIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDEL 287
           PEKDLVIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDEL
Sbjct: 234 PEKDLVIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDEL 293

Query: 288 EFQRHPNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVIL 347
           EFQRHPNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVIL
Sbjct: 294 EFQRHPNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVIL 353

Query: 348 DVVYNHTNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELI 407
           DVVYNHTNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELI
Sbjct: 354 DVVYNHTNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELI 413

Query: 408 LESLRHWVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDC 467
           LESLRHWVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDC
Sbjct: 414 LESLRHWVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDC 473

Query: 468 GGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYNVNKRKPYH 527
           GGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYNVNKRKPYH
Sbjct: 474 GGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYNVNKRKPYH 533

Query: 528 GINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRSR 587
           GINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRSR
Sbjct: 534 GINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRSR 593

Query: 588 QMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFF 647
           QMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFF
Sbjct: 594 QMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFF 653

Query: 648 SEVIKFRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAQD 707
           SEVIKFRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNA D
Sbjct: 654 SEVIKFRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAHD 713

Query: 708 YFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGLPGVGSSYNLAPYSSILLKANL 764
           YFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDG+PGVGSSYNLAPYSSILLKANL
Sbjct: 714 YFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGIPGVGSSYNLAPYSSILLKANL 769

BLAST of Pay0021495 vs. ExPASy TrEMBL
Match: A0A5D3E5B0 (Isoamylase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G005950 PE=4 SV=1)

HSP 1 Score: 1512.3 bits (3914), Expect = 0.0e+00
Identity = 711/716 (99.30%), Postives = 714/716 (99.72%), Query Frame = 0

Query: 48  KTKQGGVKLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFA 107
           +TKQGGVKLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFA
Sbjct: 54  ETKQGGVKLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFA 113

Query: 108 IFSQHATSVTICLSLDGRIDDGMLELKLDPDENRTGDIWHICIQDLQRKNVLYGYRIDGP 167
           IFSQHATSVTICLSLDGRIDDGMLE KLDPD+NRTGDIWHICIQDLQRKNVLYGYRIDGP
Sbjct: 114 IFSQHATSVTICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLQRKNVLYGYRIDGP 173

Query: 168 QGWHHGHRYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNI 227
           QGWHHGHRYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNI
Sbjct: 174 QGWHHGHRYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNI 233

Query: 228 PEKDLVIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDEL 287
           PEKDLVIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDEL
Sbjct: 234 PEKDLVIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDEL 293

Query: 288 EFQRHPNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVIL 347
           EFQRHPNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVIL
Sbjct: 294 EFQRHPNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVIL 353

Query: 348 DVVYNHTNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELI 407
           DVVYNHTNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELI
Sbjct: 354 DVVYNHTNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELI 413

Query: 408 LESLRHWVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDC 467
           LESLRHWVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDC
Sbjct: 414 LESLRHWVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDC 473

Query: 468 GGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYNVNKRKPYH 527
           GGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYNVNKRKPYH
Sbjct: 474 GGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYNVNKRKPYH 533

Query: 528 GINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRSR 587
           GINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRSR
Sbjct: 534 GINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRSR 593

Query: 588 QMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFF 647
           QMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFF
Sbjct: 594 QMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFF 653

Query: 648 SEVIKFRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAQD 707
           SEVIKFRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNA D
Sbjct: 654 SEVIKFRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAHD 713

Query: 708 YFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGLPGVGSSYNLAPYSSILLKANL 764
           YFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDG+PGVGSSYNLAPYSSILLKANL
Sbjct: 714 YFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGIPGVGSSYNLAPYSSILLKANL 769

BLAST of Pay0021495 vs. ExPASy TrEMBL
Match: A0A5A7TAT5 (Isoamylase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold92G002290 PE=4 SV=1)

HSP 1 Score: 1507.7 bits (3902), Expect = 0.0e+00
Identity = 711/717 (99.16%), Postives = 714/717 (99.58%), Query Frame = 0

Query: 48  KTKQGGVKLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFA 107
           +TKQGGVKLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFA
Sbjct: 54  ETKQGGVKLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFA 113

Query: 108 IFSQHATSVTICLSLDGRIDDGMLELKLDPDENRTGDIWHICI-QDLQRKNVLYGYRIDG 167
           IFSQHATSVTICLSLDGRIDDGMLE KLDPD+NRTGDIWHICI QDLQRKNVLYGYRIDG
Sbjct: 114 IFSQHATSVTICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQQDLQRKNVLYGYRIDG 173

Query: 168 PQGWHHGHRYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPN 227
           PQGWHHGHRYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPN
Sbjct: 174 PQGWHHGHRYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPN 233

Query: 228 IPEKDLVIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDE 287
           IPEKDLVIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDE
Sbjct: 234 IPEKDLVIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDE 293

Query: 288 LEFQRHPNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVI 347
           LEFQRHPNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVI
Sbjct: 294 LEFQRHPNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVI 353

Query: 348 LDVVYNHTNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMEL 407
           LDVVYNHTNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMEL
Sbjct: 354 LDVVYNHTNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMEL 413

Query: 408 ILESLRHWVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWD 467
           ILESLRHWVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWD
Sbjct: 414 ILESLRHWVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWD 473

Query: 468 CGGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYNVNKRKPY 527
           CGGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYNVNKRKPY
Sbjct: 474 CGGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYNVNKRKPY 533

Query: 528 HGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRS 587
           HGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRS
Sbjct: 534 HGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRS 593

Query: 588 RQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRF 647
           RQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRF
Sbjct: 594 RQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRF 653

Query: 648 FSEVIKFRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAQ 707
           FSEVIKFRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNA 
Sbjct: 654 FSEVIKFRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAH 713

Query: 708 DYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGLPGVGSSYNLAPYSSILLKANL 764
           DYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDG+PGVGSSYNLAPYSSILLKANL
Sbjct: 714 DYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGIPGVGSSYNLAPYSSILLKANL 770

BLAST of Pay0021495 vs. ExPASy TrEMBL
Match: A0A0A0LE02 (Aamy domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G889910 PE=4 SV=1)

HSP 1 Score: 1473.4 bits (3813), Expect = 0.0e+00
Identity = 691/716 (96.51%), Postives = 704/716 (98.32%), Query Frame = 0

Query: 48  KTKQGGVKLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFA 107
           +TKQGGVKLWTVSA S RDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFA
Sbjct: 55  ETKQGGVKLWTVSALSSRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFA 114

Query: 108 IFSQHATSVTICLSLDGRIDDGMLELKLDPDENRTGDIWHICIQDLQRKNVLYGYRIDGP 167
           IFSQHATSVT+CLSLDGRIDDGMLE KLDPD+NRTGDIWHICIQDL+RKNVLYGYRIDGP
Sbjct: 115 IFSQHATSVTLCLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLRRKNVLYGYRIDGP 174

Query: 168 QGWHHGHRYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNI 227
           QGWHHGHRYD GT+LLDPYAKFVEGRR+FGGENKS+GFLGTYDF+SLPFDWGNDYKLPNI
Sbjct: 175 QGWHHGHRYDAGTVLLDPYAKFVEGRRYFGGENKSTGFLGTYDFDSLPFDWGNDYKLPNI 234

Query: 228 PEKDLVIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDEL 287
           PEKDLVIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDEL
Sbjct: 235 PEKDLVIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDEL 294

Query: 288 EFQRHPNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVIL 347
           EFQRHPNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNAS EFKQMVKALHAAGIEVIL
Sbjct: 295 EFQRHPNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASLEFKQMVKALHAAGIEVIL 354

Query: 348 DVVYNHTNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELI 407
           DVVYNHTNE DDV PY TSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELI
Sbjct: 355 DVVYNHTNEADDVIPYITSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELI 414

Query: 408 LESLRHWVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDC 467
           LESLRHWVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDC
Sbjct: 415 LESLRHWVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDC 474

Query: 468 GGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYNVNKRKPYH 527
           GGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLY VNKRKP H
Sbjct: 475 GGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPCH 534

Query: 528 GINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRSR 587
           GINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALR+R
Sbjct: 535 GINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRTR 594

Query: 588 QMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFF 647
           QMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFF
Sbjct: 595 QMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFF 654

Query: 648 SEVIKFRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAQD 707
           SEVIKFRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNA +
Sbjct: 655 SEVIKFRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAHE 714

Query: 708 YFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGLPGVGSSYNLAPYSSILLKANL 764
           YFVNVSLPSPPT R+WFRVVDTNLESPHDFVLDG+PGVGSSYN+APYSSILLKANL
Sbjct: 715 YFVNVSLPSPPTKRKWFRVVDTNLESPHDFVLDGIPGVGSSYNVAPYSSILLKANL 770

BLAST of Pay0021495 vs. ExPASy TrEMBL
Match: A0A1S3CQN6 (isoamylase 3, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LOC103503684 PE=4 SV=1)

HSP 1 Score: 1440.6 bits (3728), Expect = 0.0e+00
Identity = 676/680 (99.41%), Postives = 678/680 (99.71%), Query Frame = 0

Query: 84  MLKIFPGQAFPLGVSEVDNGINFAIFSQHATSVTICLSLDGRIDDGMLELKLDPDENRTG 143
           MLKIFPGQAFPLGVSEVDNGINFAIFSQHATSVTICLSLDGRIDDGMLE KLDPD+NRTG
Sbjct: 1   MLKIFPGQAFPLGVSEVDNGINFAIFSQHATSVTICLSLDGRIDDGMLEFKLDPDDNRTG 60

Query: 144 DIWHICIQDLQRKNVLYGYRIDGPQGWHHGHRYDDGTMLLDPYAKFVEGRRFFGGENKSS 203
           DIWHICIQDLQRKNVLYGYRIDGPQGWHHGHRYDDGTMLLDPYAKFVEGRRFFGGENKSS
Sbjct: 61  DIWHICIQDLQRKNVLYGYRIDGPQGWHHGHRYDDGTMLLDPYAKFVEGRRFFGGENKSS 120

Query: 204 GFLGTYDFESLPFDWGNDYKLPNIPEKDLVIYEMNVRGFTADESSGLPSSTRGSYLGVIE 263
           GFLGTYDFESLPFDWGNDYKLPNIPEKDLVIYEMNVRGFTADESSGLPSSTRGSYLGVIE
Sbjct: 121 GFLGTYDFESLPFDWGNDYKLPNIPEKDLVIYEMNVRGFTADESSGLPSSTRGSYLGVIE 180

Query: 264 KIPHLLELGVNAVELLPVFEFDELEFQRHPNPRDHMINTWGYSTINFFAPMSRYASAGGG 323
           KIPHLLELGVNAVELLPVFEFDELEFQRHPNPRDHMINTWGYSTINFFAPMSRYASAGGG
Sbjct: 181 KIPHLLELGVNAVELLPVFEFDELEFQRHPNPRDHMINTWGYSTINFFAPMSRYASAGGG 240

Query: 324 PLNASQEFKQMVKALHAAGIEVILDVVYNHTNEGDDVNPYTTSFRGIDNKVYYMLDLKNN 383
           PLNASQEFKQMVKALHAAGIEVILDVVYNHTNEGDDVNPYTTSFRGIDNKVYYMLDLKNN
Sbjct: 241 PLNASQEFKQMVKALHAAGIEVILDVVYNHTNEGDDVNPYTTSFRGIDNKVYYMLDLKNN 300

Query: 384 GQYFNFSGCGNTLNCNHPVVMELILESLRHWVVEYHVDGFRFDLASVLCRGTDGAPLSAP 443
           GQYFNFSGCGNTLNCNHPVVMELILESLRHWVVEYHVDGFRFDLASVLCRGTDGAPLSAP
Sbjct: 301 GQYFNFSGCGNTLNCNHPVVMELILESLRHWVVEYHVDGFRFDLASVLCRGTDGAPLSAP 360

Query: 444 PLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGM 503
           PLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGM
Sbjct: 361 PLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGM 420

Query: 504 KGSFATRVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCN 563
           KGSFATRVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCN
Sbjct: 421 KGSFATRVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCN 480

Query: 564 DNFSWNCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYG 623
           DNFSWNCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYG
Sbjct: 481 DNFSWNCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYG 540

Query: 624 HDNALNHFLWEQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLNKNDITWHESNWDNPE 683
           HDNALNHFLWEQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLNKNDITWHESNWDNPE
Sbjct: 541 HDNALNHFLWEQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLNKNDITWHESNWDNPE 600

Query: 684 SKFLAYTLHDDNGEDVYLAFNAQDYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGLP 743
           SKFLAYTLHDDNGEDVYLAFNA DYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDG+P
Sbjct: 601 SKFLAYTLHDDNGEDVYLAFNAHDYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGIP 660

Query: 744 GVGSSYNLAPYSSILLKANL 764
           GVGSSYNLAPYSSILLKANL
Sbjct: 661 GVGSSYNLAPYSSILLKANL 680

BLAST of Pay0021495 vs. NCBI nr
Match: XP_008466194.1 (PREDICTED: isoamylase 3, chloroplastic isoform X1 [Cucumis melo] >XP_008466195.1 PREDICTED: isoamylase 3, chloroplastic isoform X1 [Cucumis melo] >TYK31297.1 isoamylase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 1512.3 bits (3914), Expect = 0.0e+00
Identity = 711/716 (99.30%), Postives = 714/716 (99.72%), Query Frame = 0

Query: 48  KTKQGGVKLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFA 107
           +TKQGGVKLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFA
Sbjct: 54  ETKQGGVKLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFA 113

Query: 108 IFSQHATSVTICLSLDGRIDDGMLELKLDPDENRTGDIWHICIQDLQRKNVLYGYRIDGP 167
           IFSQHATSVTICLSLDGRIDDGMLE KLDPD+NRTGDIWHICIQDLQRKNVLYGYRIDGP
Sbjct: 114 IFSQHATSVTICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLQRKNVLYGYRIDGP 173

Query: 168 QGWHHGHRYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNI 227
           QGWHHGHRYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNI
Sbjct: 174 QGWHHGHRYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNI 233

Query: 228 PEKDLVIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDEL 287
           PEKDLVIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDEL
Sbjct: 234 PEKDLVIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDEL 293

Query: 288 EFQRHPNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVIL 347
           EFQRHPNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVIL
Sbjct: 294 EFQRHPNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVIL 353

Query: 348 DVVYNHTNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELI 407
           DVVYNHTNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELI
Sbjct: 354 DVVYNHTNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELI 413

Query: 408 LESLRHWVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDC 467
           LESLRHWVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDC
Sbjct: 414 LESLRHWVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDC 473

Query: 468 GGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYNVNKRKPYH 527
           GGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYNVNKRKPYH
Sbjct: 474 GGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYNVNKRKPYH 533

Query: 528 GINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRSR 587
           GINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRSR
Sbjct: 534 GINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRSR 593

Query: 588 QMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFF 647
           QMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFF
Sbjct: 594 QMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFF 653

Query: 648 SEVIKFRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAQD 707
           SEVIKFRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNA D
Sbjct: 654 SEVIKFRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAHD 713

Query: 708 YFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGLPGVGSSYNLAPYSSILLKANL 764
           YFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDG+PGVGSSYNLAPYSSILLKANL
Sbjct: 714 YFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGIPGVGSSYNLAPYSSILLKANL 769

BLAST of Pay0021495 vs. NCBI nr
Match: KAA0038687.1 (isoamylase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 1507.7 bits (3902), Expect = 0.0e+00
Identity = 711/717 (99.16%), Postives = 714/717 (99.58%), Query Frame = 0

Query: 48  KTKQGGVKLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFA 107
           +TKQGGVKLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFA
Sbjct: 54  ETKQGGVKLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFA 113

Query: 108 IFSQHATSVTICLSLDGRIDDGMLELKLDPDENRTGDIWHICI-QDLQRKNVLYGYRIDG 167
           IFSQHATSVTICLSLDGRIDDGMLE KLDPD+NRTGDIWHICI QDLQRKNVLYGYRIDG
Sbjct: 114 IFSQHATSVTICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQQDLQRKNVLYGYRIDG 173

Query: 168 PQGWHHGHRYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPN 227
           PQGWHHGHRYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPN
Sbjct: 174 PQGWHHGHRYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPN 233

Query: 228 IPEKDLVIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDE 287
           IPEKDLVIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDE
Sbjct: 234 IPEKDLVIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDE 293

Query: 288 LEFQRHPNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVI 347
           LEFQRHPNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVI
Sbjct: 294 LEFQRHPNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVI 353

Query: 348 LDVVYNHTNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMEL 407
           LDVVYNHTNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMEL
Sbjct: 354 LDVVYNHTNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMEL 413

Query: 408 ILESLRHWVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWD 467
           ILESLRHWVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWD
Sbjct: 414 ILESLRHWVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWD 473

Query: 468 CGGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYNVNKRKPY 527
           CGGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYNVNKRKPY
Sbjct: 474 CGGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYNVNKRKPY 533

Query: 528 HGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRS 587
           HGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRS
Sbjct: 534 HGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRS 593

Query: 588 RQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRF 647
           RQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRF
Sbjct: 594 RQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRF 653

Query: 648 FSEVIKFRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAQ 707
           FSEVIKFRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNA 
Sbjct: 654 FSEVIKFRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAH 713

Query: 708 DYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGLPGVGSSYNLAPYSSILLKANL 764
           DYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDG+PGVGSSYNLAPYSSILLKANL
Sbjct: 714 DYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGIPGVGSSYNLAPYSSILLKANL 770

BLAST of Pay0021495 vs. NCBI nr
Match: XP_011652565.1 (isoamylase 3, chloroplastic isoform X1 [Cucumis sativus] >KGN60235.1 hypothetical protein Csa_002537 [Cucumis sativus])

HSP 1 Score: 1473.4 bits (3813), Expect = 0.0e+00
Identity = 691/716 (96.51%), Postives = 704/716 (98.32%), Query Frame = 0

Query: 48  KTKQGGVKLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFA 107
           +TKQGGVKLWTVSA S RDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFA
Sbjct: 55  ETKQGGVKLWTVSALSSRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFA 114

Query: 108 IFSQHATSVTICLSLDGRIDDGMLELKLDPDENRTGDIWHICIQDLQRKNVLYGYRIDGP 167
           IFSQHATSVT+CLSLDGRIDDGMLE KLDPD+NRTGDIWHICIQDL+RKNVLYGYRIDGP
Sbjct: 115 IFSQHATSVTLCLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLRRKNVLYGYRIDGP 174

Query: 168 QGWHHGHRYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNI 227
           QGWHHGHRYD GT+LLDPYAKFVEGRR+FGGENKS+GFLGTYDF+SLPFDWGNDYKLPNI
Sbjct: 175 QGWHHGHRYDAGTVLLDPYAKFVEGRRYFGGENKSTGFLGTYDFDSLPFDWGNDYKLPNI 234

Query: 228 PEKDLVIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDEL 287
           PEKDLVIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDEL
Sbjct: 235 PEKDLVIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDEL 294

Query: 288 EFQRHPNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVIL 347
           EFQRHPNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNAS EFKQMVKALHAAGIEVIL
Sbjct: 295 EFQRHPNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASLEFKQMVKALHAAGIEVIL 354

Query: 348 DVVYNHTNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELI 407
           DVVYNHTNE DDV PY TSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELI
Sbjct: 355 DVVYNHTNEADDVIPYITSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELI 414

Query: 408 LESLRHWVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDC 467
           LESLRHWVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDC
Sbjct: 415 LESLRHWVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDC 474

Query: 468 GGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYNVNKRKPYH 527
           GGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLY VNKRKP H
Sbjct: 475 GGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPCH 534

Query: 528 GINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRSR 587
           GINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALR+R
Sbjct: 535 GINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRTR 594

Query: 588 QMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFF 647
           QMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFF
Sbjct: 595 QMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFF 654

Query: 648 SEVIKFRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAQD 707
           SEVIKFRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNA +
Sbjct: 655 SEVIKFRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAHE 714

Query: 708 YFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGLPGVGSSYNLAPYSSILLKANL 764
           YFVNVSLPSPPT R+WFRVVDTNLESPHDFVLDG+PGVGSSYN+APYSSILLKANL
Sbjct: 715 YFVNVSLPSPPTKRKWFRVVDTNLESPHDFVLDGIPGVGSSYNVAPYSSILLKANL 770

BLAST of Pay0021495 vs. NCBI nr
Match: XP_008466196.1 (PREDICTED: isoamylase 3, chloroplastic isoform X2 [Cucumis melo])

HSP 1 Score: 1440.6 bits (3728), Expect = 0.0e+00
Identity = 676/680 (99.41%), Postives = 678/680 (99.71%), Query Frame = 0

Query: 84  MLKIFPGQAFPLGVSEVDNGINFAIFSQHATSVTICLSLDGRIDDGMLELKLDPDENRTG 143
           MLKIFPGQAFPLGVSEVDNGINFAIFSQHATSVTICLSLDGRIDDGMLE KLDPD+NRTG
Sbjct: 1   MLKIFPGQAFPLGVSEVDNGINFAIFSQHATSVTICLSLDGRIDDGMLEFKLDPDDNRTG 60

Query: 144 DIWHICIQDLQRKNVLYGYRIDGPQGWHHGHRYDDGTMLLDPYAKFVEGRRFFGGENKSS 203
           DIWHICIQDLQRKNVLYGYRIDGPQGWHHGHRYDDGTMLLDPYAKFVEGRRFFGGENKSS
Sbjct: 61  DIWHICIQDLQRKNVLYGYRIDGPQGWHHGHRYDDGTMLLDPYAKFVEGRRFFGGENKSS 120

Query: 204 GFLGTYDFESLPFDWGNDYKLPNIPEKDLVIYEMNVRGFTADESSGLPSSTRGSYLGVIE 263
           GFLGTYDFESLPFDWGNDYKLPNIPEKDLVIYEMNVRGFTADESSGLPSSTRGSYLGVIE
Sbjct: 121 GFLGTYDFESLPFDWGNDYKLPNIPEKDLVIYEMNVRGFTADESSGLPSSTRGSYLGVIE 180

Query: 264 KIPHLLELGVNAVELLPVFEFDELEFQRHPNPRDHMINTWGYSTINFFAPMSRYASAGGG 323
           KIPHLLELGVNAVELLPVFEFDELEFQRHPNPRDHMINTWGYSTINFFAPMSRYASAGGG
Sbjct: 181 KIPHLLELGVNAVELLPVFEFDELEFQRHPNPRDHMINTWGYSTINFFAPMSRYASAGGG 240

Query: 324 PLNASQEFKQMVKALHAAGIEVILDVVYNHTNEGDDVNPYTTSFRGIDNKVYYMLDLKNN 383
           PLNASQEFKQMVKALHAAGIEVILDVVYNHTNEGDDVNPYTTSFRGIDNKVYYMLDLKNN
Sbjct: 241 PLNASQEFKQMVKALHAAGIEVILDVVYNHTNEGDDVNPYTTSFRGIDNKVYYMLDLKNN 300

Query: 384 GQYFNFSGCGNTLNCNHPVVMELILESLRHWVVEYHVDGFRFDLASVLCRGTDGAPLSAP 443
           GQYFNFSGCGNTLNCNHPVVMELILESLRHWVVEYHVDGFRFDLASVLCRGTDGAPLSAP
Sbjct: 301 GQYFNFSGCGNTLNCNHPVVMELILESLRHWVVEYHVDGFRFDLASVLCRGTDGAPLSAP 360

Query: 444 PLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGM 503
           PLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGM
Sbjct: 361 PLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGM 420

Query: 504 KGSFATRVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCN 563
           KGSFATRVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCN
Sbjct: 421 KGSFATRVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCN 480

Query: 564 DNFSWNCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYG 623
           DNFSWNCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYG
Sbjct: 481 DNFSWNCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYG 540

Query: 624 HDNALNHFLWEQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLNKNDITWHESNWDNPE 683
           HDNALNHFLWEQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLNKNDITWHESNWDNPE
Sbjct: 541 HDNALNHFLWEQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLNKNDITWHESNWDNPE 600

Query: 684 SKFLAYTLHDDNGEDVYLAFNAQDYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGLP 743
           SKFLAYTLHDDNGEDVYLAFNA DYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDG+P
Sbjct: 601 SKFLAYTLHDDNGEDVYLAFNAHDYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGIP 660

Query: 744 GVGSSYNLAPYSSILLKANL 764
           GVGSSYNLAPYSSILLKANL
Sbjct: 661 GVGSSYNLAPYSSILLKANL 680

BLAST of Pay0021495 vs. NCBI nr
Match: XP_038896292.1 (isoamylase 3, chloroplastic isoform X1 [Benincasa hispida] >XP_038896293.1 isoamylase 3, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 1422.5 bits (3681), Expect = 0.0e+00
Identity = 669/714 (93.70%), Postives = 688/714 (96.36%), Query Frame = 0

Query: 50  KQGGVKLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIF 109
           KQGGVKL TV AF RRDQ+RVLEQEAPENLDTGPMLKIFPGQAFPLGVSEV +GINFAIF
Sbjct: 55  KQGGVKLCTVCAFGRRDQERVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVADGINFAIF 114

Query: 110 SQHATSVTICLSLDGRIDDGMLELKLDPDENRTGDIWHICIQDLQRKNVLYGYRIDGPQG 169
           SQHATSVTICLSL+ RIDD MLE KLDPD NRTGDIWHIC QDL RK+VLYGYRIDGPQG
Sbjct: 115 SQHATSVTICLSLNERIDDRMLEFKLDPDNNRTGDIWHICFQDLPRKSVLYGYRIDGPQG 174

Query: 170 WHHGHRYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNIPE 229
           WHHGHR+D  T+LLDPYAKFVEGRR+FGGENKSSGFLGTYDF+SLPFDWGNDYKLPNI E
Sbjct: 175 WHHGHRFDGSTVLLDPYAKFVEGRRYFGGENKSSGFLGTYDFDSLPFDWGNDYKLPNILE 234

Query: 230 KDLVIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEF 289
           KDLVIYEMNVRGFTADESSGL SSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEF
Sbjct: 235 KDLVIYEMNVRGFTADESSGLASSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEF 294

Query: 290 QRHPNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDV 349
           QRHPNPRDHMIN WGYSTINFFAPMSRYASAGGGPLNAS+EFK+MVKALHAAGIEVILDV
Sbjct: 295 QRHPNPRDHMINMWGYSTINFFAPMSRYASAGGGPLNASREFKEMVKALHAAGIEVILDV 354

Query: 350 VYNHTNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILE 409
           VYNHTNE DDVNPYTTSFRGIDNKVYYMLDL+NNGQY NFSGCGNTLNCNHPVVMELIL+
Sbjct: 355 VYNHTNEADDVNPYTTSFRGIDNKVYYMLDLRNNGQYLNFSGCGNTLNCNHPVVMELILD 414

Query: 410 SLRHWVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGG 469
           SLRHWVV+YHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGG
Sbjct: 415 SLRHWVVDYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGG 474

Query: 470 LYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYNVNKRKPYHGI 529
           LYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLY VNKRKPYHGI
Sbjct: 475 LYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGI 534

Query: 530 NFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRSRQM 589
           NFVIAHDGFTLRDLVSYNVKHND NGEGGNDG NDNFSWNCGFEGETEDTSIKALR+RQM
Sbjct: 535 NFVIAHDGFTLRDLVSYNVKHNDVNGEGGNDGSNDNFSWNCGFEGETEDTSIKALRTRQM 594

Query: 590 KNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSE 649
           KNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLW+QLEARK DHFRFFSE
Sbjct: 595 KNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWDQLEARKTDHFRFFSE 654

Query: 650 VIKFRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAQDYF 709
           VIKFRRKHPHLFSRENFL+KNDI+WHESNWDNPESKFLAYTLHDDNGEDVYLAFNA DY 
Sbjct: 655 VIKFRRKHPHLFSRENFLSKNDISWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAHDYS 714

Query: 710 VNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGLPGVGSSYNLAPYSSILLKANL 764
           VNVSLPSPPT RRWFRVVDTNLESPHDFVLDG+PGV S+YNLAPYSSILLKA L
Sbjct: 715 VNVSLPSPPTKRRWFRVVDTNLESPHDFVLDGIPGVESTYNLAPYSSILLKAKL 768

BLAST of Pay0021495 vs. TAIR 10
Match: AT4G09020.1 (isoamylase 3 )

HSP 1 Score: 1124.0 bits (2906), Expect = 0.0e+00
Identity = 525/711 (73.84%), Postives = 596/711 (83.83%), Query Frame = 0

Query: 58  TVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHATSVT 117
           T+    RR  +RV+E+EA    +T  + K+  G+  PLGVS+VD GINFA+FSQ+ATSVT
Sbjct: 57  TLKVTCRRAHERVVEEEASTMTET-KLFKVSSGEVSPLGVSQVDKGINFALFSQNATSVT 116

Query: 118 ICLSL-----DGRIDDGMLELKLDPDENRTGDIWHICIQDLQRKNVLYGYRIDGPQGWHH 177
           +CLSL     D   DDGM+EL LDP  N+TGD WHIC++DL   NVLYGYR+DGP  W  
Sbjct: 117 LCLSLSQSGKDDTDDDGMIELVLDPSVNKTGDTWHICVEDLPLNNVLYGYRVDGPGEWQQ 176

Query: 178 GHRYDDGTMLLDPYAKFVEGRRFFG-GENKSSGFLGTYDFESLPFDWGNDYKLPNIPEKD 237
           GHR+D   +LLDPYAK V+G   FG    K + F GTYDFES PFDWG+DYK PNIPEKD
Sbjct: 177 GHRFDRSILLLDPYAKLVKGHSSFGDSSQKFAQFYGTYDFESSPFDWGDDYKFPNIPEKD 236

Query: 238 LVIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQR 297
           LVIYEMNVR FTADESSG+  +  GSYLG IEKIPHL +LG+NAVELLPVFEFDELE QR
Sbjct: 237 LVIYEMNVRAFTADESSGMDPAIGGSYLGFIEKIPHLQDLGINAVELLPVFEFDELELQR 296

Query: 298 HPNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVY 357
             NPRDHM+NTWGYST+NFFAPMSRYAS  G P+ AS+EFK+MVKALH+AGIEVILDVVY
Sbjct: 297 RSNPRDHMVNTWGYSTVNFFAPMSRYASGEGDPIKASKEFKEMVKALHSAGIEVILDVVY 356

Query: 358 NHTNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESL 417
           NHTNE DD  PYTTSFRGIDNKVYYMLD   N Q  NFSGCGNTLNCNHPVVMELIL+SL
Sbjct: 357 NHTNEADDKYPYTTSFRGIDNKVYYMLD--PNNQLLNFSGCGNTLNCNHPVVMELILDSL 416

Query: 418 RHWVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLY 477
           RHWV EYHVDGFRFDLASVLCR TDG+PLSAPPLIRAI+KD++LSRCKIIAEPWDCGGLY
Sbjct: 417 RHWVTEYHVDGFRFDLASVLCRATDGSPLSAPPLIRAIAKDSVLSRCKIIAEPWDCGGLY 476

Query: 478 LVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYNVNKRKPYHGINF 537
           LVG+FPNWDRWAEWNG+YRDD+R+FIKGD GMKGSFATRV+GSSDLY VN+RKPYHG+NF
Sbjct: 477 LVGKFPNWDRWAEWNGMYRDDVRRFIKGDSGMKGSFATRVSGSSDLYQVNQRKPYHGVNF 536

Query: 538 VIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRSRQMKN 597
           VIAHDGFTLRDLVSYN KHN+ANGEGGNDGCNDN SWNCGFEGET D  IK+LR+RQMKN
Sbjct: 537 VIAHDGFTLRDLVSYNFKHNEANGEGGNDGCNDNHSWNCGFEGETGDAHIKSLRTRQMKN 596

Query: 598 FHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVI 657
           FHLALM SQGTPMMLMGDEYGHTRYGNNNSYGHD +LN+F W++L+A+K++HFRFFSEVI
Sbjct: 597 FHLALMISQGTPMMLMGDEYGHTRYGNNNSYGHDTSLNNFQWKELDAKKQNHFRFFSEVI 656

Query: 658 KFRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDD-NGEDVYLAFNAQDYFV 717
           KFR  H H+   ENFL + +ITWHE NWDN ESKFLA+TLHD   G D+Y+AFNA DYFV
Sbjct: 657 KFRHSH-HVLKHENFLTQGEITWHEDNWDNSESKFLAFTLHDGIGGRDIYVAFNAHDYFV 716

Query: 718 NVSLPSPPTNRRWFRVVDTNLESPHDFVLDGLPGVGSSYNLAPYSSILLKA 762
              +P PP  ++WFRV DTNLESP DFV +G+ GV  +YN+AP+SSILL++
Sbjct: 717 KALIPQPPPGKQWFRVADTNLESPDDFVREGVAGVADTYNVAPFSSILLQS 763

BLAST of Pay0021495 vs. TAIR 10
Match: AT2G39930.1 (isoamylase 1 )

HSP 1 Score: 620.5 bits (1599), Expect = 1.7e-177
Identity = 348/749 (46.46%), Postives = 450/749 (60.08%), Query Frame = 0

Query: 58  TVSAFSRR--DQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHATS 117
           ++SA  RR  + + +   E P   D      I  G   P G +  D+G+NF+++S ++ S
Sbjct: 41  SISAKDRRSNEAENIAVVEKPLKSD---RFFISDGLPSPFGPTVRDDGVNFSVYSTNSVS 100

Query: 118 VTICL-SL-DGRIDDGMLELKLDPDENRTGDIWHICIQDLQRKNVLYGYRIDGPQGWHHG 177
            TICL SL D R +    E++LDP  NRTG +WH+ ++    K++LYGYR DG      G
Sbjct: 101 ATICLISLSDLRQNKVTEEIQLDPSRNRTGHVWHVFLRG-DFKDMLYGYRFDGKFSPEEG 160

Query: 178 HRYDDGTMLLDPYAKFVEGRRFFG----GENKSSGFLGTYDFESLPFDWGNDYKLPNIPE 237
           H YD   +LLDPYAK +  R  FG     +N               FDW  D  L  +P+
Sbjct: 161 HYYDSSNILLDPYAKAIISRDEFGVLGPDDNCWPQMACMVPTREEEFDWEGDMHL-KLPQ 220

Query: 238 KDLVIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEF 297
           KDLVIYEM+VRGFT  ESS +     G+Y GV EK+ HL ELG+N +EL+P  EF+ELE+
Sbjct: 221 KDLVIYEMHVRGFTRHESSKI--EFPGTYQGVAEKLDHLKELGINCIELMPCHEFNELEY 280

Query: 298 QRHPN-PRDHMINTWGYSTINFFAPMSRYASAGGGPL--NASQEFKQMVKALHAAGIEVI 357
             +     DH +N WGYSTI FF+PM RYASA        A  EFK +VK  H  GIEVI
Sbjct: 281 YSYNTILGDHRVNFWGYSTIGFFSPMIRYASASSNNFAGRAINEFKILVKEAHKRGIEVI 340

Query: 358 LDVVYNHTNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMEL 417
           +DVV NHT EG++  P   SFRG+DN VYYML  K  G+++N+SGCGNT NCNHPVV + 
Sbjct: 341 MDVVLNHTAEGNEKGP-IFSFRGVDNSVYYMLAPK--GEFYNYSGCGNTFNCNHPVVRQF 400

Query: 418 ILESLRHWVVEYHVDGFRFDLASVLCRG-------------------TDGAPLSAPPLIR 477
           IL+ LR+WV E HVDGFRFDL S++ R                    T G P+S PP+I 
Sbjct: 401 ILDCLRYWVTEMHVDGFRFDLGSIMSRSSSLWDAANVYGADVEGDLLTTGTPISCPPVID 460

Query: 478 AISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSF 537
            IS D IL   K+IAE WD GGLY VG FP+W  W+EWNG +RD +R+FIKG  G  G+F
Sbjct: 461 MISNDPILRGVKLIAEAWDAGGLYQVGMFPHWGIWSEWNGKFRDVVRQFIKGTDGFSGAF 520

Query: 538 ATRVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFS 597
           A  + GS +LY    RKP+H INF+ AHDGFTL DLV+YN K+N ANGE  NDG N N+S
Sbjct: 521 AECLCGSPNLYQ-GGRKPWHSINFICAHDGFTLADLVTYNNKNNLANGEENNDGENHNYS 580

Query: 598 WNCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNA 657
           WNCG EG+    S+K LR RQM+NF ++LM SQG PM+ MGDEYGHT+ GNNN+Y HDN 
Sbjct: 581 WNCGEEGDFASISVKRLRKRQMRNFFVSLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNY 640

Query: 658 LNHFLWEQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLNKNDITWHE-----SNWDNP 717
           +N+F W++ E    D FRF   +IKFR +   L    +F     + WH       NW + 
Sbjct: 641 MNYFRWDKKEEAHSDFFRFCRILIKFRDECESL-GLNDFPTAKRLQWHGLAPEIPNW-SE 700

Query: 718 ESKFLAYTLHDDNGEDVYLAFNAQDYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGL 760
            S+F+A++L D   +++Y+AFN       VSLP+ P   RW   VDT+  SP+D +   L
Sbjct: 701 TSRFVAFSLVDSVKKEIYVAFNTSHLATLVSLPNRP-GYRWEPFVDTSKPSPYDCITPDL 760

BLAST of Pay0021495 vs. TAIR 10
Match: AT1G03310.2 (debranching enzyme 1 )

HSP 1 Score: 386.0 bits (990), Expect = 7.2e-107
Identity = 255/706 (36.12%), Postives = 361/706 (51.13%), Query Frame = 0

Query: 90  GQAFPLGVSE--VDNGINFAIFSQHATSVTICLSLDGRIDDGMLELKLDPDENRTGDIWH 149
           G   PLG+S    D+  NF+ FS+ +T+V +CL  D   D   LEL LDP  NRTGD+WH
Sbjct: 230 GHPLPLGLSSGPDDDSWNFSFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWH 289

Query: 150 ICIQDLQRKNVLYGYRIDGPQGWHHGHRYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLG 209
             + D     V YGYR             +   ++LDPYA  V       G++ S  +LG
Sbjct: 290 ASV-DNTWDFVRYGYRCKETAHSKEDVDVEGEPIVLDPYATVV-------GKSVSQKYLG 349

Query: 210 TYDFESLPFDWGNDYKLPNIPEKDLVIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPH 269
           +   +S  FDWG D   PNIP + L++Y +NV+GFT   SS LPS+  G++ GV EK+ H
Sbjct: 350 SLS-KSPSFDWGEDVS-PNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSH 409

Query: 270 LLELGVNAVELLPVFEFDELEFQRHPNPRDHMINTWGYSTINFFAPMSRYASAGGGPLN- 329
           L  LG NAV L P+F F E   Q+ P           Y   +FF+PM  Y     GP N 
Sbjct: 410 LKTLGTNAVLLEPIFSFSE---QKGP-----------YFPFHFFSPMDIY-----GPSNS 469

Query: 330 ---ASQEFKQMVKALHAAGIEVILDVVYNHTNEGDDVNPYTTSFRGIDNKVYYMLDLKNN 389
              A    K MVK LH+ GIEV+L+VV+ HT +       + + RGID+  YY     N+
Sbjct: 470 LESAVNSMKVMVKKLHSEGIEVLLEVVFTHTAD-------SGALRGIDDSSYYYKGRAND 529

Query: 390 GQYFNFSGCGNTLNCNHPVVMELILESLRHWVVEYHVDGFRFDLASVLCRGTDGAPLSAP 449
               ++      LNCN+PVV +L+LESLR+WV E+HVDGF F  AS L RG  G  LS P
Sbjct: 530 LDSKSY------LNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSLLRGVHGEQLSRP 589

Query: 450 PLIRAISKDAILSRCKIIAEPWD-CGGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCG 509
           PL+ AI+ D +L+  K+IA+ WD    +    RFP+W RWAE N  Y  ++R F++G  G
Sbjct: 590 PLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNTRYCRNVRNFLRGR-G 649

Query: 510 MKGSFATRVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGC 569
           +    ATR+ GS D++  + R P    N++  + G +L D+VS++               
Sbjct: 650 VLSDLATRICGSGDVF-TDGRGPAFSFNYISRNSGLSLVDIVSFS-----------GPEL 709

Query: 570 NDNFSWNCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSY 629
               SWNCG EG T  +++   R +Q++NF      S G P++ MGDE G +  G+    
Sbjct: 710 ASELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLL- 769

Query: 630 GHDNALNHFLWEQL-EARKRDHFRFFSEVIKFRRKHPHLFSRENFLNKNDITWHESN--- 689
               +   F W  L  A      +F S +   R +   +F R +FL   +I W+ ++   
Sbjct: 770 ---ESRKPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTT 829

Query: 690 --WDNPESKFLAYTLHDDNGE---------------DVYLAFNAQDYFVNVSLPSPPTNR 749
             W++P SKFLA  +  ++ E               D+++ FNA D+  +V LPS P   
Sbjct: 830 PKWEDPASKFLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGS 876

Query: 750 RWFRVVDTNLESPHDFVLDGLPGVGSS-------YNLAPYSSILLK 761
           +W R+VDT L  P  F ++G   V          Y + PYS  L +
Sbjct: 890 KWRRLVDTALPFPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFE 876

BLAST of Pay0021495 vs. TAIR 10
Match: AT1G03310.1 (debranching enzyme 1 )

HSP 1 Score: 386.0 bits (990), Expect = 7.2e-107
Identity = 255/706 (36.12%), Postives = 361/706 (51.13%), Query Frame = 0

Query: 90  GQAFPLGVSE--VDNGINFAIFSQHATSVTICLSLDGRIDDGMLELKLDPDENRTGDIWH 149
           G   PLG+S    D+  NF+ FS+ +T+V +CL  D   D   LEL LDP  NRTGD+WH
Sbjct: 230 GHPLPLGLSSGPDDDSWNFSFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWH 289

Query: 150 ICIQDLQRKNVLYGYRIDGPQGWHHGHRYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLG 209
             + D     V YGYR             +   ++LDPYA  V       G++ S  +LG
Sbjct: 290 ASV-DNTWDFVRYGYRCKETAHSKEDVDVEGEPIVLDPYATVV-------GKSVSQKYLG 349

Query: 210 TYDFESLPFDWGNDYKLPNIPEKDLVIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPH 269
           +   +S  FDWG D   PNIP + L++Y +NV+GFT   SS LPS+  G++ GV EK+ H
Sbjct: 350 SLS-KSPSFDWGEDVS-PNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSH 409

Query: 270 LLELGVNAVELLPVFEFDELEFQRHPNPRDHMINTWGYSTINFFAPMSRYASAGGGPLN- 329
           L  LG NAV L P+F F E   Q+ P           Y   +FF+PM  Y     GP N 
Sbjct: 410 LKTLGTNAVLLEPIFSFSE---QKGP-----------YFPFHFFSPMDIY-----GPSNS 469

Query: 330 ---ASQEFKQMVKALHAAGIEVILDVVYNHTNEGDDVNPYTTSFRGIDNKVYYMLDLKNN 389
              A    K MVK LH+ GIEV+L+VV+ HT +       + + RGID+  YY     N+
Sbjct: 470 LESAVNSMKVMVKKLHSEGIEVLLEVVFTHTAD-------SGALRGIDDSSYYYKGRAND 529

Query: 390 GQYFNFSGCGNTLNCNHPVVMELILESLRHWVVEYHVDGFRFDLASVLCRGTDGAPLSAP 449
               ++      LNCN+PVV +L+LESLR+WV E+HVDGF F  AS L RG  G  LS P
Sbjct: 530 LDSKSY------LNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSLLRGVHGEQLSRP 589

Query: 450 PLIRAISKDAILSRCKIIAEPWD-CGGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCG 509
           PL+ AI+ D +L+  K+IA+ WD    +    RFP+W RWAE N  Y  ++R F++G  G
Sbjct: 590 PLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNTRYCRNVRNFLRGR-G 649

Query: 510 MKGSFATRVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGC 569
           +    ATR+ GS D++  + R P    N++  + G +L D+VS++               
Sbjct: 650 VLSDLATRICGSGDVF-TDGRGPAFSFNYISRNSGLSLVDIVSFS-----------GPEL 709

Query: 570 NDNFSWNCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSY 629
               SWNCG EG T  +++   R +Q++NF      S G P++ MGDE G +  G+    
Sbjct: 710 ASELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLL- 769

Query: 630 GHDNALNHFLWEQL-EARKRDHFRFFSEVIKFRRKHPHLFSRENFLNKNDITWHESN--- 689
               +   F W  L  A      +F S +   R +   +F R +FL   +I W+ ++   
Sbjct: 770 ---ESRKPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTT 829

Query: 690 --WDNPESKFLAYTLHDDNGE---------------DVYLAFNAQDYFVNVSLPSPPTNR 749
             W++P SKFLA  +  ++ E               D+++ FNA D+  +V LPS P   
Sbjct: 830 PKWEDPASKFLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGS 876

Query: 750 RWFRVVDTNLESPHDFVLDGLPGVGSS-------YNLAPYSSILLK 761
           +W R+VDT L  P  F ++G   V          Y + PYS  L +
Sbjct: 890 KWRRLVDTALPFPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFE 876

BLAST of Pay0021495 vs. TAIR 10
Match: AT5G04360.1 (limit dextrinase )

HSP 1 Score: 122.9 bits (307), Expect = 1.1e-27
Identity = 112/424 (26.42%), Postives = 180/424 (42.45%), Query Frame = 0

Query: 94  PLGVSEVDNGINFAIFSQHATSVTICLSLDGRIDDGMLELKLDPDENRTGDIWHICIQDL 153
           PLG      G++  +++  A +V++C+     +D   +E+    + N        C    
Sbjct: 207 PLGAHFTPEGVSLHLWAPTAQAVSVCI-YKNPLDKSPMEICPLKEANGVWSTEGAC---- 266

Query: 154 QRKNVLYGYRIDGPQGWHHGHRYDDGTMLL------DPYAK--FVEGRRFFGGENKSSGF 213
                 Y Y++           Y   TM L      DPYA+    +GR+          F
Sbjct: 267 SWGGCYYVYKVS---------VYHPSTMKLETCYANDPYARGLSADGRK---------TF 326

Query: 214 LGTYDFESL-PFDWGN--DYKLPNIPEKDLVIYEMNVRGFTADESSGLPSSTRGSYLGVI 273
           L   D + L P  W N  D K       D+ IYE++VR F+A++ +  P + RG YL   
Sbjct: 327 LVNLDSDDLKPEGWDNLADKKPCLRSFSDISIYELHVRDFSANDETVEPEN-RGGYLAFT 386

Query: 274 EK-------IPHLLELGVNAVELLPVFEFDELEFQR--------------HPNPRDHMIN 333
            K       +  L++ G+  + LLP F+F +++ ++               P+  +    
Sbjct: 387 SKDSAGVKHLQKLVDAGLTHLHLLPTFQFGDVDDEKENWKSVDTSLLEGLRPDSTEAQAR 446

Query: 334 ----------TWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVY 393
                      WGY+ + +  P   YAS   GP     EF++MV+AL+  G+ V+LDVVY
Sbjct: 447 ITEIQNDDGYNWGYNPVLWGVPKGSYASDPTGPCRII-EFRKMVQALNCTGLNVVLDVVY 506

Query: 394 NHTNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESL 453
           NH +     +  +   + +    YY+   +N+  +   S C N     H +V  LI + L
Sbjct: 507 NHLHASGPHDKESVLDKIVPG--YYL--RRNSDGFIENSTCVNNTASEHYMVDRLIRDDL 566

Query: 454 RHWVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLY 476
            +WVV Y VDGFRFDL   + + T     SA   +R  +     SR  +  E W+ G + 
Sbjct: 567 LNWVVNYKVDGFRFDLMGHIMKATIVNAKSAIGSLRKETDGVDGSRIYLYGEGWNFGEVA 601