MS015000 (gene) Bitter gourd (TR) v1

Overview


Name	MS015000
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	ABC transporter B family member 15-like
Location	scaffold2: 462954 .. 463148 (+)
RNA-Seq Expression	MS015000
Synteny	MS015000

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCATGAAGAAAAGTGGCCTTTTCAGATATGCTGATGGGGTTGATAAGTTTCTGCTTTTATTGGGTTCTTTGGGGAGCATTGGAGATGGGCTTACAACCCCACTCACTATGCTTGTTCTTAGTGGTATGATCAATCAGTATTCTCGCTCTAATCCTAACACTTTTTCCAATCAAGTGGTGGACAAGGTACTT

mRNA sequence

ATGGGCATGAAGAAAAGTGGCCTTTTCAGATATGCTGATGGGGTTGATAAGTTTCTGCTTTTATTGGGTTCTTTGGGGAGCATTGGAGATGGGCTTACAACCCCACTCACTATGCTTGTTCTTAGTGGTATGATCAATCAGTATTCTCGCTCTAATCCTAACACTTTTTCCAATCAAGTGGTGGACAAGGTACTT

Coding sequence (CDS)

ATGGGCATGAAGAAAAGTGGCCTTTTCAGATATGCTGATGGGGTTGATAAGTTTCTGCTTTTATTGGGTTCTTTGGGGAGCATTGGAGATGGGCTTACAACCCCACTCACTATGCTTGTTCTTAGTGGTATGATCAATCAGTATTCTCGCTCTAATCCTAACACTTTTTCCAATCAAGTGGTGGACAAGGTACTT

Protein sequence

MGMKKSGLFRYADGVDKFLLLLGSLGSIGDGLTTPLTMLVLSGMINQYSRSNPNTFSNQVVDKVL

Homology

[Close]

BLAST of MS015000 vs. NCBI nr
Match: XP_022151783.1 (ABC transporter B family member 15-like [Momordica charantia])

HSP 1 Score: 122.9 bits (307), Expect = 1.0e-24
Identity = 62/63 (98.41%), Postives = 62/63 (98.41%), Query Frame = 0


Query: 1  MGMKKSGLFRYADGVDKFLLLLGSLGSIGDGLTTPLTMLVLSGMINQYSRSNPNTFSNQV 60
          MGMKKSGLFRYADGVDKFLLLLGSLGSIGDGLTTPLTMLVLSGMINQYSRSNPNT SNQV
Sbjct: 1  MGMKKSGLFRYADGVDKFLLLLGSLGSIGDGLTTPLTMLVLSGMINQYSRSNPNTSSNQV 60

Query: 61 VDK 64
          VDK
Sbjct: 61 VDK 63

[Close]

BLAST of MS015000 vs. NCBI nr
Match: TYK15677.1 (putative multidrug resistance protein [Cucumis melo var. makuwa])

HSP 1 Score: 96.3 bits (238), Expect = 1.0e-16
Identity = 48/63 (76.19%), Postives = 54/63 (85.71%), Query Frame = 0


Query: 1  MGMKKSGLFRYADGVDKFLLLLGSLGSIGDGLTTPLTMLVLSGMINQYSRSNPNTFSNQV 60
          MG KK+G+FRYAD VD  L++LG LGSIGDGLTTPLTMLVLSGMIN YS S+ N+FSN V
Sbjct: 1  MGKKKNGVFRYADWVDMLLMVLGCLGSIGDGLTTPLTMLVLSGMINHYSVSDSNSFSNHV 60

Query: 61 VDK 64
          VDK
Sbjct: 61 VDK 63

[Close]

BLAST of MS015000 vs. NCBI nr
Match: KAA0034243.1 (putative multidrug resistance protein [Cucumis melo var. makuwa])

HSP 1 Score: 96.3 bits (238), Expect = 1.0e-16
Identity = 48/63 (76.19%), Postives = 54/63 (85.71%), Query Frame = 0


Query: 1  MGMKKSGLFRYADGVDKFLLLLGSLGSIGDGLTTPLTMLVLSGMINQYSRSNPNTFSNQV 60
          MG KK+G+FRYAD VD  L++LG LGSIGDGLTTPLTMLVLSGMIN YS S+ N+FSN V
Sbjct: 1  MGKKKNGVFRYADWVDMLLMVLGCLGSIGDGLTTPLTMLVLSGMINHYSVSDSNSFSNHV 60

Query: 61 VDK 64
          VDK
Sbjct: 61 VDK 63

[Close]

BLAST of MS015000 vs. NCBI nr
Match: XP_008446126.1 (PREDICTED: putative multidrug resistance protein [Cucumis melo])

HSP 1 Score: 96.3 bits (238), Expect = 1.0e-16
Identity = 48/63 (76.19%), Postives = 54/63 (85.71%), Query Frame = 0


Query: 1  MGMKKSGLFRYADGVDKFLLLLGSLGSIGDGLTTPLTMLVLSGMINQYSRSNPNTFSNQV 60
          MG KK+G+FRYAD VD  L++LG LGSIGDGLTTPLTMLVLSGMIN YS S+ N+FSN V
Sbjct: 1  MGKKKNGVFRYADWVDMLLMVLGCLGSIGDGLTTPLTMLVLSGMINHYSVSDSNSFSNHV 60

Query: 61 VDK 64
          VDK
Sbjct: 61 VDK 63

[Close]

BLAST of MS015000 vs. NCBI nr
Match: XP_004135503.1 (ABC transporter B family member 15 [Cucumis sativus] >KAE8648690.1 hypothetical protein Csa_008119 [Cucumis sativus])

HSP 1 Score: 95.5 bits (236), Expect = 1.8e-16
Identity = 47/63 (74.60%), Postives = 54/63 (85.71%), Query Frame = 0


Query: 1  MGMKKSGLFRYADGVDKFLLLLGSLGSIGDGLTTPLTMLVLSGMINQYSRSNPNTFSNQV 60
          MG KK+G+FRYAD +D+ L+ LG LGSIGDGLTTPLTMLVLSGMIN YS S+ N+FSN V
Sbjct: 1  MGKKKTGVFRYADWLDQLLMFLGCLGSIGDGLTTPLTMLVLSGMINHYSVSDSNSFSNHV 60

Query: 61 VDK 64
          VDK
Sbjct: 61 VDK 63

[Close]

BLAST of MS015000 vs. ExPASy Swiss-Prot
Match: Q9LHD1 (ABC transporter B family member 15 OS=Arabidopsis thaliana OX=3702 GN=ABCB15 PE=3 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 1.5e-05
Identity = 23/48 (47.92%), Postives = 34/48 (70.83%), Query Frame = 0


Query: 8  LFRYADGVDKFLLLLGSLGSIGDGLTTPLTMLVLSGMINQYSRSNPNT 56
          +F +ADGVD  L+ LG +G++GDG TTPL +L+ S ++N    S+ NT
Sbjct: 23 IFMHADGVDWLLMGLGLIGAVGDGFTTPLVLLITSKLMNNIGGSSFNT 70

[Close]

BLAST of MS015000 vs. ExPASy Swiss-Prot
Match: Q9FWX8 (ABC transporter B family member 12 OS=Arabidopsis thaliana OX=3702 GN=ABCB12 PE=2 SV=2)

HSP 1 Score: 47.0 bits (110), Expect = 9.5e-05
Identity = 23/57 (40.35%), Postives = 35/57 (61.40%), Query Frame = 0


Query: 8  LFRYADGVDKFLLLLGSLGSIGDGLTTPLTMLVLSGMINQYSRSNPNTFSNQVVDKV 65
          LF +AD  D FL++ GSLG+IG+G+  PL  L+   +I+ + ++  N     VV KV
Sbjct: 33 LFAFADSFDVFLMICGSLGAIGNGVCLPLMTLLFGDLIDSFGKNQNNKDIVDVVSKV 89

[Close]

BLAST of MS015000 vs. ExPASy Swiss-Prot
Match: Q9LHK4 (Putative ABC transporter B family member 8 OS=Arabidopsis thaliana OX=3702 GN=ABCB8 PE=5 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 1.2e-04
Identity = 21/50 (42.00%), Postives = 40/50 (80.00%), Query Frame = 0


Query: 8  LFRYADGVDKFLLLLGSLGSIGDGLTTPLTMLVLSGMINQ--YSRSNPNT 56
          +FR+AD +D  L++LGS+G+IGDG++T ++++ +S ++N   YS+ NP++
Sbjct: 21 IFRFADWIDIVLMVLGSVGAIGDGMSTNVSLVFVSRIMNTLGYSQHNPSS 70

[Close]

BLAST of MS015000 vs. ExPASy Swiss-Prot
Match: Q9LSJ6 (ABC transporter B family member 17 OS=Arabidopsis thaliana OX=3702 GN=ABCB17 PE=3 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 1.6e-04
Identity = 22/60 (36.67%), Postives = 36/60 (60.00%), Query Frame = 0


Query: 8  LFRYADGVDKFLLLLGSLGSIGDGLTTPLTMLVLSGMINQY--SRSNPNTFSNQVVDKVL 66
          +F +ADGVD  L+ LG +G++GDG  TP+ + + + ++N    S SN  TF   +   V+
Sbjct: 23 IFMHADGVDWILMALGLIGAVGDGFITPVVVFIFNTLLNNLGTSSSNNKTFMQTISKNVV 82

[Close]

BLAST of MS015000 vs. ExPASy Swiss-Prot
Match: O80725 (ABC transporter B family member 4 OS=Arabidopsis thaliana OX=3702 GN=ABCB4 PE=1 SV=1)

HSP 1 Score: 44.3 bits (103), Expect = 6.2e-04
Identity = 21/48 (43.75%), Postives = 31/48 (64.58%), Query Frame = 0


Query: 8  LFRYADGVDKFLLLLGSLGSIGDGLTTPLTMLVLSGMINQYSRSNPNT 56
          LF +AD  D  L++LG+LGSIG+GL  PL  L+   +I+ +  +  NT
Sbjct: 51 LFAFADSFDFLLMILGTLGSIGNGLGFPLMTLLFGDLIDAFGENQTNT 98

[Close]

BLAST of MS015000 vs. ExPASy TrEMBL
Match: A0A6J1DD49 (ABC transporter B family member 15-like OS=Momordica charantia OX=3673 GN=LOC111019682 PE=4 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 5.1e-25
Identity = 62/63 (98.41%), Postives = 62/63 (98.41%), Query Frame = 0


Query: 1  MGMKKSGLFRYADGVDKFLLLLGSLGSIGDGLTTPLTMLVLSGMINQYSRSNPNTFSNQV 60
          MGMKKSGLFRYADGVDKFLLLLGSLGSIGDGLTTPLTMLVLSGMINQYSRSNPNT SNQV
Sbjct: 1  MGMKKSGLFRYADGVDKFLLLLGSLGSIGDGLTTPLTMLVLSGMINQYSRSNPNTSSNQV 60

Query: 61 VDK 64
          VDK
Sbjct: 61 VDK 63

[Close]

BLAST of MS015000 vs. ExPASy TrEMBL
Match: A0A0A0KS41 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G593370 PE=4 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 1.7e-17
Identity = 48/65 (73.85%), Postives = 56/65 (86.15%), Query Frame = 0


Query: 1  MGMKKSGLFRYADGVDKFLLLLGSLGSIGDGLTTPLTMLVLSGMINQYSRSNPNTFSNQV 60
          MG KK+G+FRYAD +D+ L+ LG LGSIGDGLTTPLTMLVLSGMIN YS S+ N+FSN V
Sbjct: 1  MGKKKTGVFRYADWLDQLLMFLGCLGSIGDGLTTPLTMLVLSGMINHYSVSDSNSFSNHV 60

Query: 61 VDKVL 66
          VDKV+
Sbjct: 61 VDKVI 65

[Close]

BLAST of MS015000 vs. ExPASy TrEMBL
Match: A0A5A7SUU5 (Putative multidrug resistance protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G002420 PE=4 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 5.1e-17
Identity = 48/63 (76.19%), Postives = 54/63 (85.71%), Query Frame = 0


Query: 1  MGMKKSGLFRYADGVDKFLLLLGSLGSIGDGLTTPLTMLVLSGMINQYSRSNPNTFSNQV 60
          MG KK+G+FRYAD VD  L++LG LGSIGDGLTTPLTMLVLSGMIN YS S+ N+FSN V
Sbjct: 1  MGKKKNGVFRYADWVDMLLMVLGCLGSIGDGLTTPLTMLVLSGMINHYSVSDSNSFSNHV 60

Query: 61 VDK 64
          VDK
Sbjct: 61 VDK 63

[Close]

BLAST of MS015000 vs. ExPASy TrEMBL
Match: A0A5D3CUV1 (Putative multidrug resistance protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001740 PE=4 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 5.1e-17
Identity = 48/63 (76.19%), Postives = 54/63 (85.71%), Query Frame = 0


Query: 1  MGMKKSGLFRYADGVDKFLLLLGSLGSIGDGLTTPLTMLVLSGMINQYSRSNPNTFSNQV 60
          MG KK+G+FRYAD VD  L++LG LGSIGDGLTTPLTMLVLSGMIN YS S+ N+FSN V
Sbjct: 1  MGKKKNGVFRYADWVDMLLMVLGCLGSIGDGLTTPLTMLVLSGMINHYSVSDSNSFSNHV 60

Query: 61 VDK 64
          VDK
Sbjct: 61 VDK 63

[Close]

BLAST of MS015000 vs. ExPASy TrEMBL
Match: A0A1S3BEB7 (putative multidrug resistance protein OS=Cucumis melo OX=3656 GN=LOC103488944 PE=4 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 5.1e-17
Identity = 48/63 (76.19%), Postives = 54/63 (85.71%), Query Frame = 0


Query: 1  MGMKKSGLFRYADGVDKFLLLLGSLGSIGDGLTTPLTMLVLSGMINQYSRSNPNTFSNQV 60
          MG KK+G+FRYAD VD  L++LG LGSIGDGLTTPLTMLVLSGMIN YS S+ N+FSN V
Sbjct: 1  MGKKKNGVFRYADWVDMLLMVLGCLGSIGDGLTTPLTMLVLSGMINHYSVSDSNSFSNHV 60

Query: 61 VDK 64
          VDK
Sbjct: 61 VDK 63

[Close]

BLAST of MS015000 vs. TAIR 10
Match: AT3G28345.1 (ABC transporter family protein )

HSP 1 Score: 49.7 bits (117), Expect = 1.0e-06
Identity = 23/48 (47.92%), Postives = 34/48 (70.83%), Query Frame = 0


Query: 8  LFRYADGVDKFLLLLGSLGSIGDGLTTPLTMLVLSGMINQYSRSNPNT 56
          +F +ADGVD  L+ LG +G++GDG TTPL +L+ S ++N    S+ NT
Sbjct: 23 IFMHADGVDWLLMGLGLIGAVGDGFTTPLVLLITSKLMNNIGGSSFNT 70

[Close]

BLAST of MS015000 vs. TAIR 10
Match: AT1G02530.1 (P-glycoprotein 12 )

HSP 1 Score: 47.0 bits (110), Expect = 6.8e-06
Identity = 23/57 (40.35%), Postives = 35/57 (61.40%), Query Frame = 0


Query: 8  LFRYADGVDKFLLLLGSLGSIGDGLTTPLTMLVLSGMINQYSRSNPNTFSNQVVDKV 65
          LF +AD  D FL++ GSLG+IG+G+  PL  L+   +I+ + ++  N     VV KV
Sbjct: 33 LFAFADSFDVFLMICGSLGAIGNGVCLPLMTLLFGDLIDSFGKNQNNKDIVDVVSKV 89

[Close]

BLAST of MS015000 vs. TAIR 10
Match: AT3G28380.1 (P-glycoprotein 17 )

HSP 1 Score: 46.2 bits (108), Expect = 1.2e-05
Identity = 22/60 (36.67%), Postives = 36/60 (60.00%), Query Frame = 0


Query: 8  LFRYADGVDKFLLLLGSLGSIGDGLTTPLTMLVLSGMINQY--SRSNPNTFSNQVVDKVL 66
          +F +ADGVD  L+ LG +G++GDG  TP+ + + + ++N    S SN  TF   +   V+
Sbjct: 23 IFMHADGVDWILMALGLIGAVGDGFITPVVVFIFNTLLNNLGTSSSNNKTFMQTISKNVV 82

[Close]

BLAST of MS015000 vs. TAIR 10
Match: AT2G47000.1 (ATP binding cassette subfamily B4 )

HSP 1 Score: 44.3 bits (103), Expect = 4.4e-05
Identity = 21/48 (43.75%), Postives = 31/48 (64.58%), Query Frame = 0


Query: 8  LFRYADGVDKFLLLLGSLGSIGDGLTTPLTMLVLSGMINQYSRSNPNT 56
          LF +AD  D  L++LG+LGSIG+GL  PL  L+   +I+ +  +  NT
Sbjct: 51 LFAFADSFDFLLMILGTLGSIGNGLGFPLMTLLFGDLIDAFGENQTNT 98

[Close]

BLAST of MS015000 vs. TAIR 10
Match: AT1G02520.1 (P-glycoprotein 11 )

HSP 1 Score: 43.1 bits (100), Expect = 9.8e-05
Identity = 20/57 (35.09%), Postives = 34/57 (59.65%), Query Frame = 0


Query: 8   LFRYADGVDKFLLLLGSLGSIGDGLTTPLTMLVLSGMINQYSRSNPNTFSNQVVDKV 65
           LF +AD  D  L++ GS+G+IG+G++ P   L+   +I+ + ++  N     VV KV
Sbjct: 46  LFAFADSSDVLLMICGSIGAIGNGMSLPFMTLLFGDLIDSFGKNQNNKDIVDVVSKV 102

The following BLAST results are available for this feature:

BLAST of MS015000 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022151783.1	1.0e-24	98.41	ABC transporter B family member 15-like [Momordica charantia]	[more]
TYK15677.1	1.0e-16	76.19	putative multidrug resistance protein [Cucumis melo var. makuwa]	[more]
KAA0034243.1	1.0e-16	76.19	putative multidrug resistance protein [Cucumis melo var. makuwa]	[more]
XP_008446126.1	1.0e-16	76.19	PREDICTED: putative multidrug resistance protein [Cucumis melo]	[more]
XP_004135503.1	1.8e-16	74.60	ABC transporter B family member 15 [Cucumis sativus] >KAE8648690.1 hypothetical ...	[more]

BLAST of MS015000 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LHD1	1.5e-05	47.92	ABC transporter B family member 15 OS=Arabidopsis thaliana OX=3702 GN=ABCB15 PE=...	[more]
Q9FWX8	9.5e-05	40.35	ABC transporter B family member 12 OS=Arabidopsis thaliana OX=3702 GN=ABCB12 PE=...	[more]
Q9LHK4	1.2e-04	42.00	Putative ABC transporter B family member 8 OS=Arabidopsis thaliana OX=3702 GN=AB...	[more]
Q9LSJ6	1.6e-04	36.67	ABC transporter B family member 17 OS=Arabidopsis thaliana OX=3702 GN=ABCB17 PE=...	[more]
O80725	6.2e-04	43.75	ABC transporter B family member 4 OS=Arabidopsis thaliana OX=3702 GN=ABCB4 PE=1 ...	[more]

BLAST of MS015000 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DD49	5.1e-25	98.41	ABC transporter B family member 15-like OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A0A0KS41	1.7e-17	73.85	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G593370 PE=4 SV=1	[more]
A0A5A7SUU5	5.1e-17	76.19	Putative multidrug resistance protein OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A5D3CUV1	5.1e-17	76.19	Putative multidrug resistance protein OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A1S3BEB7	5.1e-17	76.19	putative multidrug resistance protein OS=Cucumis melo OX=3656 GN=LOC103488944 PE...	[more]

BLAST of MS015000 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G28345.1	1.0e-06	47.92	ABC transporter family protein	[more]
AT1G02530.1	6.8e-06	40.35	P-glycoprotein 12	[more]
AT3G28380.1	1.2e-05	36.67	P-glycoprotein 17	[more]
AT2G47000.1	4.4e-05	43.75	ATP binding cassette subfamily B4	[more]
AT1G02520.1	9.8e-05	35.09	P-glycoprotein 11	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036640	ABC transporter type 1, transmembrane domain superfamily	GENE3D	1.20.1560.10	ABC transporter type 1, transmembrane domain	coord: 3..64 e-value: 5.7E-9 score: 36.6
None	No IPR available	PANTHER	PTHR24222:SF48	ABC TRANSPORTER B FAMILY MEMBER 15	coord: 5..64
None	No IPR available	PANTHER	PTHR24222	ABC TRANSPORTER B FAMILY	coord: 5..64

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS015000.1	MS015000.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005524	ATP binding