MS014918 (gene) Bitter gourd (TR) v1

Overview


Name	MS014918
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	serine/threonine-protein kinase-like protein At5g23170
Location	scaffold2: 22257 .. 22727 (+)
RNA-Seq Expression	MS014918
Synteny	MS014918

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTTATTTGAGTACCAGCAGCTTTTATACGCCACAGATGGGTTCTCTCCTGCCAGGCTCATAGGCAAAGGATCGCATGGATGGGTTTACAAAGCAATACTGGAGGATGATACGGTGGTGGCCGTGAAGAAGCCATTAGAGAGCAACTCCAAGAAGCTGGAGAACGAAGCGCGAGTGTTGTGTGGTCTAGGCCACAACCCGCTGGTGGTTAACTTTCTTGGAACCAGCGTTGAGAAGAAGAGGCGGCTTCAGCTTCTTGTTATGGAGTTCATGCCCAACGGGAGCCTTCACGATTTGGTTGCGGCGCCCAATTCCGTGACTTGGCCCAAACGCCTCCAAATCATCACGCAGATTGCGCGTGCCGTCCAATTCCTTCACGAGGACCTGGTCATCCACCGAGACATCAAGCCCCAAAACATCCTCTTCGATTCCCATTGGAATCCCAAATTGGCGGATTTCGGGCTCGCC

mRNA sequence

ATGGAGTTATTTGAGTACCAGCAGCTTTTATACGCCACAGATGGGTTCTCTCCTGCCAGGCTCATAGGCAAAGGATCGCATGGATGGGTTTACAAAGCAATACTGGAGGATGATACGGTGGTGGCCGTGAAGAAGCCATTAGAGAGCAACTCCAAGAAGCTGGAGAACGAAGCGCGAGTGTTGTGTGGTCTAGGCCACAACCCGCTGGTGGTTAACTTTCTTGGAACCAGCGTTGAGAAGAAGAGGCGGCTTCAGCTTCTTGTTATGGAGTTCATGCCCAACGGGAGCCTTCACGATTTGGTTGCGGCGCCCAATTCCGTGACTTGGCCCAAACGCCTCCAAATCATCACGCAGATTGCGCGTGCCGTCCAATTCCTTCACGAGGACCTGGTCATCCACCGAGACATCAAGCCCCAAAACATCCTCTTCGATTCCCATTGGAATCCCAAATTGGCGGATTTCGGGCTCGCC

Coding sequence (CDS)

ATGGAGTTATTTGAGTACCAGCAGCTTTTATACGCCACAGATGGGTTCTCTCCTGCCAGGCTCATAGGCAAAGGATCGCATGGATGGGTTTACAAAGCAATACTGGAGGATGATACGGTGGTGGCCGTGAAGAAGCCATTAGAGAGCAACTCCAAGAAGCTGGAGAACGAAGCGCGAGTGTTGTGTGGTCTAGGCCACAACCCGCTGGTGGTTAACTTTCTTGGAACCAGCGTTGAGAAGAAGAGGCGGCTTCAGCTTCTTGTTATGGAGTTCATGCCCAACGGGAGCCTTCACGATTTGGTTGCGGCGCCCAATTCCGTGACTTGGCCCAAACGCCTCCAAATCATCACGCAGATTGCGCGTGCCGTCCAATTCCTTCACGAGGACCTGGTCATCCACCGAGACATCAAGCCCCAAAACATCCTCTTCGATTCCCATTGGAATCCCAAATTGGCGGATTTCGGGCTCGCC

Protein sequence

MELFEYQQLLYATDGFSPARLIGKGSHGWVYKAILEDDTVVAVKKPLESNSKKLENEARVLCGLGHNPLVVNFLGTSVEKKRRLQLLVMEFMPNGSLHDLVAAPNSVTWPKRLQIITQIARAVQFLHEDLVIHRDIKPQNILFDSHWNPKLADFGLA

Homology

[Close]

BLAST of MS014918 vs. NCBI nr
Match: XP_022151785.1 (serine/threonine-protein kinase-like protein At5g23170 [Momordica charantia])

HSP 1 Score: 325.1 bits (832), Expect = 3.3e-85
Identity = 157/157 (100.00%), Postives = 157/157 (100.00%), Query Frame = 0


Query: 1   MELFEYQQLLYATDGFSPARLIGKGSHGWVYKAILEDDTVVAVKKPLESNSKKLENEARV 60
           MELFEYQQLLYATDGFSPARLIGKGSHGWVYKAILEDDTVVAVKKPLESNSKKLENEARV
Sbjct: 1   MELFEYQQLLYATDGFSPARLIGKGSHGWVYKAILEDDTVVAVKKPLESNSKKLENEARV 60

Query: 61  LCGLGHNPLVVNFLGTSVEKKRRLQLLVMEFMPNGSLHDLVAAPNSVTWPKRLQIITQIA 120
           LCGLGHNPLVVNFLGTSVEKKRRLQLLVMEFMPNGSLHDLVAAPNSVTWPKRLQIITQIA
Sbjct: 61  LCGLGHNPLVVNFLGTSVEKKRRLQLLVMEFMPNGSLHDLVAAPNSVTWPKRLQIITQIA 120

Query: 121 RAVQFLHEDLVIHRDIKPQNILFDSHWNPKLADFGLA 158
           RAVQFLHEDLVIHRDIKPQNILFDSHWNPKLADFGLA
Sbjct: 121 RAVQFLHEDLVIHRDIKPQNILFDSHWNPKLADFGLA 157

[Close]

BLAST of MS014918 vs. NCBI nr
Match: XP_022998265.1 (serine/threonine-protein kinase-like protein At5g23170 [Cucurbita maxima])

HSP 1 Score: 227.6 bits (579), Expect = 7.3e-56
Identity = 121/160 (75.62%), Postives = 134/160 (83.75%), Query Frame = 0


Query: 1   MELFEYQQLLYATDGFSPARLIGKGSHGWVYKAILEDDTVVAVKKPLESNS-KKLENEAR 60
           +E FEYQQLL ATDGFS A LIGKGSHG VYKAIL+D+TVVAVKKPL++N   KLENEAR
Sbjct: 2   IEFFEYQQLLCATDGFSAASLIGKGSHGSVYKAILKDNTVVAVKKPLQTNDYSKLENEAR 61

Query: 61  VLCGLGHNPLVVNFLGTSVEKKRRLQLLVMEFMPNGSLHDLVAAPNSVTWPKRLQIITQI 120
           VL  L   PLVVNFLGTS++     +LLVMEFMPNGSLHDL+ AP  VTWPKRLQIITQI
Sbjct: 62  VLWSLRQTPLVVNFLGTSLDSANN-KLLVMEFMPNGSLHDLLLAP--VTWPKRLQIITQI 121

Query: 121 ARAVQFLHE--DLVIHRDIKPQNILFDSHWNPKLADFGLA 158
           ARAV+FLH+   LVIHRDIK +NILFDS+WN KLADFGLA
Sbjct: 122 ARAVEFLHQANPLVIHRDIKAENILFDSNWNLKLADFGLA 158

[Close]

BLAST of MS014918 vs. NCBI nr
Match: XP_038877861.1 (serine/threonine-protein kinase-like protein At5g23170 [Benincasa hispida])

HSP 1 Score: 226.1 bits (575), Expect = 2.1e-55
Identity = 117/163 (71.78%), Postives = 136/163 (83.44%), Query Frame = 0


Query: 1   MELFEYQQLLYATDGFSPARLIGKGSHGWVYKAILEDDTVVAVKKP-LESNSKKLENEAR 60
           +ELFEYQQLLYATDGFSPAR+IGKGSHGWVYKAILED+ VVAVKKP LES+  K+ENEAR
Sbjct: 2   IELFEYQQLLYATDGFSPARIIGKGSHGWVYKAILEDNRVVAVKKPLLESSYLKVENEAR 61

Query: 61  VLCGLGHNPLVVNFLGTSVEKKR-RLQLLVMEFMPNGSLHDLVAAPN--SVTWPKRLQII 120
           VL  L  +PLVVNFLGT+++    + +LLVMEF+PNGSLHDL+ AP    + W +RL II
Sbjct: 62  VLWSLRQSPLVVNFLGTTLDSPTYKYKLLVMEFLPNGSLHDLLLAPEPAPIPWTRRLHII 121

Query: 121 TQIARAVQFLHE--DLVIHRDIKPQNILFDSHWNPKLADFGLA 158
           TQIARAV FLH+   LVIHRDIK +NILFDS+W+ KLADFGLA
Sbjct: 122 TQIARAVHFLHQANPLVIHRDIKAENILFDSNWDLKLADFGLA 164

[Close]

BLAST of MS014918 vs. NCBI nr
Match: KAG6601619.1 (Zinc finger transcription factor YY1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 225.7 bits (574), Expect = 2.8e-55
Identity = 120/160 (75.00%), Postives = 133/160 (83.12%), Query Frame = 0


Query: 1   MELFEYQQLLYATDGFSPARLIGKGSHGWVYKAILEDDTVVAVKKPLESNS-KKLENEAR 60
           +E F+YQ LL ATDGFS A LIGKGSHG VYKAIL+D+TVVAVKKPL+SN   KLENEAR
Sbjct: 2   IEFFDYQHLLCATDGFSAASLIGKGSHGSVYKAILKDNTVVAVKKPLQSNDYSKLENEAR 61

Query: 61  VLCGLGHNPLVVNFLGTSVEKKRRLQLLVMEFMPNGSLHDLVAAPNSVTWPKRLQIITQI 120
           VL  L   PLVVNFLGTS++     +LLVMEFMPNGSLHDL+ AP  VTWPKRLQIITQI
Sbjct: 62  VLWSLRQTPLVVNFLGTSLDSANN-KLLVMEFMPNGSLHDLLLAP--VTWPKRLQIITQI 121

Query: 121 ARAVQFLHE--DLVIHRDIKPQNILFDSHWNPKLADFGLA 158
           ARAV+FLH+   LVIHRDIK +NILFDS+WN KLADFGLA
Sbjct: 122 ARAVEFLHQANPLVIHRDIKAENILFDSNWNLKLADFGLA 158

[Close]

BLAST of MS014918 vs. NCBI nr
Match: KAG7032378.1 (Serine/threonine-protein kinase-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 225.7 bits (574), Expect = 2.8e-55
Identity = 120/160 (75.00%), Postives = 133/160 (83.12%), Query Frame = 0


Query: 1   MELFEYQQLLYATDGFSPARLIGKGSHGWVYKAILEDDTVVAVKKPLESNS-KKLENEAR 60
           +E F+YQ LL ATDGFS A LIGKGSHG VYKAIL+D+TVVAVKKPL+SN   KLENEAR
Sbjct: 2   IEFFDYQHLLCATDGFSAASLIGKGSHGSVYKAILKDNTVVAVKKPLQSNDYSKLENEAR 61

Query: 61  VLCGLGHNPLVVNFLGTSVEKKRRLQLLVMEFMPNGSLHDLVAAPNSVTWPKRLQIITQI 120
           VL  L   PLVVNFLGTS++     +LLVMEFMPNGSLHDL+ AP  VTWPKRLQIITQI
Sbjct: 62  VLWSLRQTPLVVNFLGTSLDSANN-KLLVMEFMPNGSLHDLLLAP--VTWPKRLQIITQI 121

Query: 121 ARAVQFLHE--DLVIHRDIKPQNILFDSHWNPKLADFGLA 158
           ARAV+FLH+   LVIHRDIK +NILFDS+WN KLADFGLA
Sbjct: 122 ARAVEFLHQANPLVIHRDIKAENILFDSNWNLKLADFGLA 158

[Close]

BLAST of MS014918 vs. ExPASy Swiss-Prot
Match: Q9FMY3 (Serine/threonine-protein kinase-like protein At5g23170 OS=Arabidopsis thaliana OX=3702 GN=At5g23170 PE=2 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 3.0e-36
Identity = 90/180 (50.00%), Postives = 116/180 (64.44%), Query Frame = 0


Query: 1   MELFEYQQLLYATDGFSPARLIGKGSHGWVYKAILE----DDT---VVAVKKP------- 60
           M+ F+Y +L+ A D FSP++LIGKGSHG+VYKA+L     D+T   VVA+K P       
Sbjct: 1   MKEFDYDKLVTAIDRFSPSKLIGKGSHGYVYKALLHHQDVDETRQRVVAIKTPSSLSPSS 60

Query: 61  -------LESNSKKLENEARVLCGLGHNPLVVNFLGTSVEKKRRLQLLVMEFMPNGSLHD 120
                      +KKLENE  V+  L ++P V++FLG   EKK    L+V+E+MPN SL+ 
Sbjct: 61  PSSSSSSKSEQTKKLENEIDVMSSLPYHPHVLSFLG-HAEKK----LMVVEYMPNESLYQ 120

Query: 121 L--VAAPNSVTWPKRLQIITQIARAVQFLHEDLVIHRDIKPQNILFDSHWNPKLADFGLA 158
           L  V+     TW KR++I  QIA AV FLHE  +IHRDIK +NILFDS+W  KLADFGLA
Sbjct: 121 LLHVSTDPLPTWLKRIEIALQIASAVHFLHEHGIIHRDIKSENILFDSNWEAKLADFGLA 175

[Close]

BLAST of MS014918 vs. ExPASy Swiss-Prot
Match: Q9SGN7 (Serine/threonine-protein kinase-like protein At1g28390 OS=Arabidopsis thaliana OX=3702 GN=At1g28390 PE=2 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 1.6e-29
Identity = 72/173 (41.62%), Postives = 106/173 (61.27%), Query Frame = 0


Query: 1   MELFEYQQLLYATDGFSPARLIGKGSHGWVYKAILEDDTVVAVKKPL---------ESNS 60
           + +F Y +L  AT+GFS    +GKGSHG VYKA+L+D  ++A  K            +N 
Sbjct: 37  LRVFNYDELAVATNGFSANNFLGKGSHGRVYKAVLDDGKLLAAVKRTTITTTVGNNNNNV 96

Query: 61  KKLENEARVLCGLGHNPLVVNFLGTSVEKKRRLQLLVMEFMPNGSLHDLVAAPNSV---- 120
            +++NE  +L  + H   +VN +G  V+ +R+ +LLV+E+MPNG+LHD + + +S+    
Sbjct: 97  SQVDNEIEILSRVRHR-WMVNLIGYCVDHRRKTKLLVVEYMPNGTLHDQLHSRSSLDSRL 156

Query: 121 -TWPKRLQIITQIARAVQFLH--EDLVIHRDIKPQNILFDSHWNPKLADFGLA 158
            +W +R++   QIA AV  LH  E  VIHRDIK  N+L D   N +LADFGLA
Sbjct: 157 SSWNRRIKHALQIAIAVHALHTAETQVIHRDIKSCNVLIDGDGNARLADFGLA 208

[Close]

BLAST of MS014918 vs. ExPASy Swiss-Prot
Match: Q9S7D9 (Serine/threonine-protein kinase-like protein CCR1 OS=Arabidopsis thaliana OX=3702 GN=CCR1 PE=1 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 1.1e-27
Identity = 66/164 (40.24%), Postives = 102/164 (62.20%), Query Frame = 0


Query: 2   ELFEYQQLLYATDGFSPARLIGKGSHGWVYKAILEDDTVVAVKKP-----LESNSKKLEN 61
           ++F   +L  AT+GF     +G+GS+G+VYKA+L D   VAVK+      + +N+++ E 
Sbjct: 506 QVFRLSELKDATNGFKEFNELGRGSYGFVYKAVLADGRQVAVKRANAATIIHTNTREFET 565

Query: 62  EARVLCGLGHNPLVVNFLGTSVEKKRRLQLLVMEFMPNGSLHD-LVAAPNSVTWPKRLQI 121
           E  +LC + H   +VN LG S E   R  LLV E+MP+G+LHD L +  + ++W  R++I
Sbjct: 566 ELEILCNIRHCN-IVNLLGYSTEMGER--LLVYEYMPHGTLHDHLHSGFSPLSWSLRIKI 625

Query: 122 ITQIARAVQFLH---EDLVIHRDIKPQNILFDSHWNPKLADFGL 157
             Q A+ +++LH   E  +IH D+K  N+L DS W  ++ADFGL
Sbjct: 626 AMQTAKGLEYLHNEAEPRIIHGDVKSSNVLLDSEWVARVADFGL 666

[Close]

BLAST of MS014918 vs. ExPASy Swiss-Prot
Match: P0C5E2 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 OS=Arabidopsis thaliana OX=3702 GN=LRK10L-1.2 PE=2 SV=3)

HSP 1 Score: 123.2 bits (308), Expect = 2.5e-27
Identity = 68/164 (41.46%), Postives = 101/164 (61.59%), Query Frame = 0


Query: 1   MELFEYQQLLYATDGFSPARLIGKGSHGWVYKAILEDDTVVAVKKPLESNSKKLE---NE 60
           + +F Y++L  AT+ F P++ +G G  G VY   L+D   VAVK+  ++N K+ E   NE
Sbjct: 326 VHIFSYEELEEATNNFDPSKELGDGGFGTVYYGKLKDGRSVAVKRLYDNNFKRAEQFRNE 385

Query: 61  ARVLCGLGHNPLVVNFLGTSVEKKRRLQLLVMEFMPNGS----LHDLVAAPNSVTWPKRL 120
             +L GL H P +V   G S ++ R L LLV E++ NG+    LH   A P+S+ W  RL
Sbjct: 386 VEILTGLRH-PNLVALFGCSSKQSRDL-LLVYEYVANGTLADHLHGPQANPSSLPWSIRL 445

Query: 121 QIITQIARAVQFLHEDLVIHRDIKPQNILFDSHWNPKLADFGLA 158
           +I  + A A+++LH   +IHRD+K  NIL D ++N K+ADFGL+
Sbjct: 446 KIAVETASALKYLHASKIIHRDVKSNNILLDQNFNVKVADFGLS 487

[Close]

BLAST of MS014918 vs. ExPASy Swiss-Prot
Match: O80963 (Serine/threonine-protein kinase-like protein CCR2 OS=Arabidopsis thaliana OX=3702 GN=CCR2 PE=1 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 7.4e-27
Identity = 68/164 (41.46%), Postives = 98/164 (59.76%), Query Frame = 0


Query: 2   ELFEYQQLLYATDGFSPARLIGKGSHGWVYKAILEDDTVVAVKKP-----LESNSKKLEN 61
           ++F   +L  AT GF     +G+GS G+VYKA+L D   VAVK+      + SN++  E+
Sbjct: 505 KIFRLSELKDATHGFKEFNELGRGSFGFVYKAVLSDGIHVAVKRANAATIIHSNNRGFES 564

Query: 62  EARVLCGLGHNPLVVNFLGTSVEKKRRLQLLVMEFMPNGSLHD-LVAAPNSVTWPKRLQI 121
           E  +LC + HN  +VN LG   E   R  LLV E+MP+G+LHD L    + + W  RL+I
Sbjct: 565 ELEILCKIRHNN-IVNLLGYCSEMGER--LLVYEYMPHGTLHDHLHGDLSQLDWSMRLKI 624

Query: 122 ITQIARAVQFLHEDL---VIHRDIKPQNILFDSHWNPKLADFGL 157
           + Q AR + +LH ++   +IHRD+K  NIL D     ++ADFGL
Sbjct: 625 MLQAARGLDYLHNEVDPPIIHRDVKTSNILLDGEMCARIADFGL 665

[Close]

BLAST of MS014918 vs. ExPASy TrEMBL
Match: A0A6J1DC59 (serine/threonine-protein kinase-like protein At5g23170 OS=Momordica charantia OX=3673 GN=LOC111019684 PE=4 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 1.6e-85
Identity = 157/157 (100.00%), Postives = 157/157 (100.00%), Query Frame = 0


Query: 1   MELFEYQQLLYATDGFSPARLIGKGSHGWVYKAILEDDTVVAVKKPLESNSKKLENEARV 60
           MELFEYQQLLYATDGFSPARLIGKGSHGWVYKAILEDDTVVAVKKPLESNSKKLENEARV
Sbjct: 1   MELFEYQQLLYATDGFSPARLIGKGSHGWVYKAILEDDTVVAVKKPLESNSKKLENEARV 60

Query: 61  LCGLGHNPLVVNFLGTSVEKKRRLQLLVMEFMPNGSLHDLVAAPNSVTWPKRLQIITQIA 120
           LCGLGHNPLVVNFLGTSVEKKRRLQLLVMEFMPNGSLHDLVAAPNSVTWPKRLQIITQIA
Sbjct: 61  LCGLGHNPLVVNFLGTSVEKKRRLQLLVMEFMPNGSLHDLVAAPNSVTWPKRLQIITQIA 120

Query: 121 RAVQFLHEDLVIHRDIKPQNILFDSHWNPKLADFGLA 158
           RAVQFLHEDLVIHRDIKPQNILFDSHWNPKLADFGLA
Sbjct: 121 RAVQFLHEDLVIHRDIKPQNILFDSHWNPKLADFGLA 157

[Close]

BLAST of MS014918 vs. ExPASy TrEMBL
Match: A0A6J1KC22 (serine/threonine-protein kinase-like protein At5g23170 OS=Cucurbita maxima OX=3661 GN=LOC111492953 PE=3 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 3.5e-56
Identity = 121/160 (75.62%), Postives = 134/160 (83.75%), Query Frame = 0


Query: 1   MELFEYQQLLYATDGFSPARLIGKGSHGWVYKAILEDDTVVAVKKPLESNS-KKLENEAR 60
           +E FEYQQLL ATDGFS A LIGKGSHG VYKAIL+D+TVVAVKKPL++N   KLENEAR
Sbjct: 2   IEFFEYQQLLCATDGFSAASLIGKGSHGSVYKAILKDNTVVAVKKPLQTNDYSKLENEAR 61

Query: 61  VLCGLGHNPLVVNFLGTSVEKKRRLQLLVMEFMPNGSLHDLVAAPNSVTWPKRLQIITQI 120
           VL  L   PLVVNFLGTS++     +LLVMEFMPNGSLHDL+ AP  VTWPKRLQIITQI
Sbjct: 62  VLWSLRQTPLVVNFLGTSLDSANN-KLLVMEFMPNGSLHDLLLAP--VTWPKRLQIITQI 121

Query: 121 ARAVQFLHE--DLVIHRDIKPQNILFDSHWNPKLADFGLA 158
           ARAV+FLH+   LVIHRDIK +NILFDS+WN KLADFGLA
Sbjct: 122 ARAVEFLHQANPLVIHRDIKAENILFDSNWNLKLADFGLA 158

[Close]

BLAST of MS014918 vs. ExPASy TrEMBL
Match: A0A6J1GY00 (serine/threonine-protein kinase-like protein At5g23170 OS=Cucurbita moschata OX=3662 GN=LOC111458232 PE=3 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 3.0e-55
Identity = 119/160 (74.38%), Postives = 133/160 (83.12%), Query Frame = 0


Query: 1   MELFEYQQLLYATDGFSPARLIGKGSHGWVYKAILEDDTVVAVKKPLESNS-KKLENEAR 60
           +E F+YQ LL ATDGFS A LIGKGSHG VYKAIL+D+TVVAVKKPL++N   KLENEAR
Sbjct: 2   IEFFDYQHLLCATDGFSAASLIGKGSHGSVYKAILKDNTVVAVKKPLQTNDYSKLENEAR 61

Query: 61  VLCGLGHNPLVVNFLGTSVEKKRRLQLLVMEFMPNGSLHDLVAAPNSVTWPKRLQIITQI 120
           VL  L   PLVVNFLGTS++     +LLVMEFMPNGSLHDL+ AP  VTWPKRLQIITQI
Sbjct: 62  VLWSLRQTPLVVNFLGTSLDSANN-KLLVMEFMPNGSLHDLLLAP--VTWPKRLQIITQI 121

Query: 121 ARAVQFLHE--DLVIHRDIKPQNILFDSHWNPKLADFGLA 158
           ARAV+FLH+   LVIHRDIK +NILFDS+WN KLADFGLA
Sbjct: 122 ARAVEFLHQANPLVIHRDIKAENILFDSNWNLKLADFGLA 158

[Close]

BLAST of MS014918 vs. ExPASy TrEMBL
Match: A0A5A7SUM7 (Serine/threonine-protein kinase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G002600 PE=4 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 2.0e-51
Identity = 112/166 (67.47%), Postives = 133/166 (80.12%), Query Frame = 0


Query: 1   MELFEYQQLLYATDGFSPARLIGKGSHGWVYKAILEDDTVVAVKKPL-ESNSKKLENEAR 60
           +ELFEYQQLLYATDGFS  RLIGKGSHGWVYKAIL+D+ VVAVKKPL E++  K++NEAR
Sbjct: 2   IELFEYQQLLYATDGFSSTRLIGKGSHGWVYKAILDDNRVVAVKKPLSETHYSKVDNEAR 61

Query: 61  VLCGLGHNPLVVNFLGTSVEK-KRRLQLLVMEFMPNGSLHDLVAAP-----NSVTWPKRL 120
           VL  L  +PLVVNFLGT+++    + +LLVMEFMPNGSL DL+  P      + +W KRL
Sbjct: 62  VLLSLRPSPLVVNFLGTTLDSPSYKNKLLVMEFMPNGSLRDLLLEPMLAPVPTTSWHKRL 121

Query: 121 QIITQIARAVQFLHE--DLVIHRDIKPQNILFDSHWNPKLADFGLA 158
            IITQIARAV FLH+   +VIHRDIK +NILFDS+W+ KLADFGLA
Sbjct: 122 HIITQIARAVCFLHQANPIVIHRDIKAENILFDSNWDLKLADFGLA 167

[Close]

BLAST of MS014918 vs. ExPASy TrEMBL
Match: A0A1S3BEU9 (serine/threonine-protein kinase-like protein At5g23170 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103488857 PE=4 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 2.0e-51
Identity = 112/166 (67.47%), Postives = 133/166 (80.12%), Query Frame = 0


Query: 1   MELFEYQQLLYATDGFSPARLIGKGSHGWVYKAILEDDTVVAVKKPL-ESNSKKLENEAR 60
           +ELFEYQQLLYATDGFS  RLIGKGSHGWVYKAIL+D+ VVAVKKPL E++  K++NEAR
Sbjct: 2   IELFEYQQLLYATDGFSSTRLIGKGSHGWVYKAILDDNRVVAVKKPLSETHYSKVDNEAR 61

Query: 61  VLCGLGHNPLVVNFLGTSVEK-KRRLQLLVMEFMPNGSLHDLVAAP-----NSVTWPKRL 120
           VL  L  +PLVVNFLGT+++    + +LLVMEFMPNGSL DL+  P      + +W KRL
Sbjct: 62  VLLSLRPSPLVVNFLGTTLDSPSYKNKLLVMEFMPNGSLRDLLLEPMLAPVPTTSWHKRL 121

Query: 121 QIITQIARAVQFLHE--DLVIHRDIKPQNILFDSHWNPKLADFGLA 158
            IITQIARAV FLH+   +VIHRDIK +NILFDS+W+ KLADFGLA
Sbjct: 122 HIITQIARAVCFLHQANPIVIHRDIKAENILFDSNWDLKLADFGLA 167

[Close]

BLAST of MS014918 vs. TAIR 10
Match: AT5G23170.1 (Protein kinase superfamily protein )

HSP 1 Score: 152.9 bits (385), Expect = 2.1e-37
Identity = 90/180 (50.00%), Postives = 116/180 (64.44%), Query Frame = 0


Query: 1   MELFEYQQLLYATDGFSPARLIGKGSHGWVYKAILE----DDT---VVAVKKP------- 60
           M+ F+Y +L+ A D FSP++LIGKGSHG+VYKA+L     D+T   VVA+K P       
Sbjct: 1   MKEFDYDKLVTAIDRFSPSKLIGKGSHGYVYKALLHHQDVDETRQRVVAIKTPSSLSPSS 60

Query: 61  -------LESNSKKLENEARVLCGLGHNPLVVNFLGTSVEKKRRLQLLVMEFMPNGSLHD 120
                      +KKLENE  V+  L ++P V++FLG   EKK    L+V+E+MPN SL+ 
Sbjct: 61  PSSSSSSKSEQTKKLENEIDVMSSLPYHPHVLSFLG-HAEKK----LMVVEYMPNESLYQ 120

Query: 121 L--VAAPNSVTWPKRLQIITQIARAVQFLHEDLVIHRDIKPQNILFDSHWNPKLADFGLA 158
           L  V+     TW KR++I  QIA AV FLHE  +IHRDIK +NILFDS+W  KLADFGLA
Sbjct: 121 LLHVSTDPLPTWLKRIEIALQIASAVHFLHEHGIIHRDIKSENILFDSNWEAKLADFGLA 175

[Close]

BLAST of MS014918 vs. TAIR 10
Match: AT1G28390.1 (Protein kinase superfamily protein )

HSP 1 Score: 130.6 bits (327), Expect = 1.1e-30
Identity = 72/173 (41.62%), Postives = 106/173 (61.27%), Query Frame = 0


Query: 1   MELFEYQQLLYATDGFSPARLIGKGSHGWVYKAILEDDTVVAVKKPL---------ESNS 60
           + +F Y +L  AT+GFS    +GKGSHG VYKA+L+D  ++A  K            +N 
Sbjct: 37  LRVFNYDELAVATNGFSANNFLGKGSHGRVYKAVLDDGKLLAAVKRTTITTTVGNNNNNV 96

Query: 61  KKLENEARVLCGLGHNPLVVNFLGTSVEKKRRLQLLVMEFMPNGSLHDLVAAPNSV---- 120
            +++NE  +L  + H   +VN +G  V+ +R+ +LLV+E+MPNG+LHD + + +S+    
Sbjct: 97  SQVDNEIEILSRVRHR-WMVNLIGYCVDHRRKTKLLVVEYMPNGTLHDQLHSRSSLDSRL 156

Query: 121 -TWPKRLQIITQIARAVQFLH--EDLVIHRDIKPQNILFDSHWNPKLADFGLA 158
            +W +R++   QIA AV  LH  E  VIHRDIK  N+L D   N +LADFGLA
Sbjct: 157 SSWNRRIKHALQIAIAVHALHTAETQVIHRDIKSCNVLIDGDGNARLADFGLA 208

[Close]

BLAST of MS014918 vs. TAIR 10
Match: AT1G28390.2 (Protein kinase superfamily protein )

HSP 1 Score: 130.6 bits (327), Expect = 1.1e-30
Identity = 72/173 (41.62%), Postives = 106/173 (61.27%), Query Frame = 0


Query: 1   MELFEYQQLLYATDGFSPARLIGKGSHGWVYKAILEDDTVVAVKKPL---------ESNS 60
           + +F Y +L  AT+GFS    +GKGSHG VYKA+L+D  ++A  K            +N 
Sbjct: 37  LRVFNYDELAVATNGFSANNFLGKGSHGRVYKAVLDDGKLLAAVKRTTITTTVGNNNNNV 96

Query: 61  KKLENEARVLCGLGHNPLVVNFLGTSVEKKRRLQLLVMEFMPNGSLHDLVAAPNSV---- 120
            +++NE  +L  + H   +VN +G  V+ +R+ +LLV+E+MPNG+LHD + + +S+    
Sbjct: 97  SQVDNEIEILSRVRHR-WMVNLIGYCVDHRRKTKLLVVEYMPNGTLHDQLHSRSSLDSRL 156

Query: 121 -TWPKRLQIITQIARAVQFLH--EDLVIHRDIKPQNILFDSHWNPKLADFGLA 158
            +W +R++   QIA AV  LH  E  VIHRDIK  N+L D   N +LADFGLA
Sbjct: 157 SSWNRRIKHALQIAIAVHALHTAETQVIHRDIKSCNVLIDGDGNARLADFGLA 208

[Close]

BLAST of MS014918 vs. TAIR 10
Match: AT3G09780.1 (CRINKLY4 related 1 )

HSP 1 Score: 124.4 bits (311), Expect = 8.1e-29
Identity = 66/164 (40.24%), Postives = 102/164 (62.20%), Query Frame = 0


Query: 2   ELFEYQQLLYATDGFSPARLIGKGSHGWVYKAILEDDTVVAVKKP-----LESNSKKLEN 61
           ++F   +L  AT+GF     +G+GS+G+VYKA+L D   VAVK+      + +N+++ E 
Sbjct: 506 QVFRLSELKDATNGFKEFNELGRGSYGFVYKAVLADGRQVAVKRANAATIIHTNTREFET 565

Query: 62  EARVLCGLGHNPLVVNFLGTSVEKKRRLQLLVMEFMPNGSLHD-LVAAPNSVTWPKRLQI 121
           E  +LC + H   +VN LG S E   R  LLV E+MP+G+LHD L +  + ++W  R++I
Sbjct: 566 ELEILCNIRHCN-IVNLLGYSTEMGER--LLVYEYMPHGTLHDHLHSGFSPLSWSLRIKI 625

Query: 122 ITQIARAVQFLH---EDLVIHRDIKPQNILFDSHWNPKLADFGL 157
             Q A+ +++LH   E  +IH D+K  N+L DS W  ++ADFGL
Sbjct: 626 AMQTAKGLEYLHNEAEPRIIHGDVKSSNVLLDSEWVARVADFGL 666

[Close]

BLAST of MS014918 vs. TAIR 10
Match: AT1G18390.1 (Protein kinase superfamily protein )

HSP 1 Score: 123.2 bits (308), Expect = 1.8e-28
Identity = 68/164 (41.46%), Postives = 101/164 (61.59%), Query Frame = 0


Query: 1   MELFEYQQLLYATDGFSPARLIGKGSHGWVYKAILEDDTVVAVKKPLESNSKKLE---NE 60
           + +F Y++L  AT+ F P++ +G G  G VY   L+D   VAVK+  ++N K+ E   NE
Sbjct: 323 VHIFSYEELEEATNNFDPSKELGDGGFGTVYYGKLKDGRSVAVKRLYDNNFKRAEQFRNE 382

Query: 61  ARVLCGLGHNPLVVNFLGTSVEKKRRLQLLVMEFMPNGS----LHDLVAAPNSVTWPKRL 120
             +L GL H P +V   G S ++ R L LLV E++ NG+    LH   A P+S+ W  RL
Sbjct: 383 VEILTGLRH-PNLVALFGCSSKQSRDL-LLVYEYVANGTLADHLHGPQANPSSLPWSIRL 442

Query: 121 QIITQIARAVQFLHEDLVIHRDIKPQNILFDSHWNPKLADFGLA 158
           +I  + A A+++LH   +IHRD+K  NIL D ++N K+ADFGL+
Sbjct: 443 KIAVETASALKYLHASKIIHRDVKSNNILLDQNFNVKVADFGLS 484

The following BLAST results are available for this feature:

BLAST of MS014918 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022151785.1	3.3e-85	100.00	serine/threonine-protein kinase-like protein At5g23170 [Momordica charantia]	[more]
XP_022998265.1	7.3e-56	75.63	serine/threonine-protein kinase-like protein At5g23170 [Cucurbita maxima]	[more]
XP_038877861.1	2.1e-55	71.78	serine/threonine-protein kinase-like protein At5g23170 [Benincasa hispida]	[more]
KAG6601619.1	2.8e-55	75.00	Zinc finger transcription factor YY1, partial [Cucurbita argyrosperma subsp. sor...	[more]
KAG7032378.1	2.8e-55	75.00	Serine/threonine-protein kinase-like protein, partial [Cucurbita argyrosperma su...	[more]

BLAST of MS014918 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FMY3	3.0e-36	50.00	Serine/threonine-protein kinase-like protein At5g23170 OS=Arabidopsis thaliana O...	[more]
Q9SGN7	1.6e-29	41.62	Serine/threonine-protein kinase-like protein At1g28390 OS=Arabidopsis thaliana O...	[more]
Q9S7D9	1.1e-27	40.24	Serine/threonine-protein kinase-like protein CCR1 OS=Arabidopsis thaliana OX=370...	[more]
P0C5E2	2.5e-27	41.46	LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 OS=A...	[more]
O80963	7.4e-27	41.46	Serine/threonine-protein kinase-like protein CCR2 OS=Arabidopsis thaliana OX=370...	[more]

BLAST of MS014918 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DC59	1.6e-85	100.00	serine/threonine-protein kinase-like protein At5g23170 OS=Momordica charantia OX...	[more]
A0A6J1KC22	3.5e-56	75.63	serine/threonine-protein kinase-like protein At5g23170 OS=Cucurbita maxima OX=36...	[more]
A0A6J1GY00	3.0e-55	74.38	serine/threonine-protein kinase-like protein At5g23170 OS=Cucurbita moschata OX=...	[more]
A0A5A7SUM7	2.0e-51	67.47	Serine/threonine-protein kinase-like protein OS=Cucumis melo var. makuwa OX=1194...	[more]
A0A1S3BEU9	2.0e-51	67.47	serine/threonine-protein kinase-like protein At5g23170 isoform X1 OS=Cucumis mel...	[more]

BLAST of MS014918 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G23170.1	2.1e-37	50.00	Protein kinase superfamily protein	[more]
AT1G28390.1	1.1e-30	41.62	Protein kinase superfamily protein	[more]
AT1G28390.2	1.1e-30	41.62	Protein kinase superfamily protein	[more]
AT3G09780.1	8.1e-29	40.24	CRINKLY4 related 1	[more]
AT1G18390.1	1.8e-28	41.46	Protein kinase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 16..157 e-value: 5.5E-7 score: -35.8
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 19..157 e-value: 4.9E-30 score: 104.8
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 16..157 score: 28.230558
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 1..157 e-value: 2.2E-44 score: 153.6
None	No IPR available	PIRSR	PIRSR000620-1	PIRSR000620-1	coord: 19..156 e-value: 1.2E-7 score: 28.8
None	No IPR available	PANTHER	PTHR47989	OS01G0750732 PROTEIN	coord: 1..157
None	No IPR available	PANTHER	PTHR47989:SF39	SUBFAMILY NOT NAMED	coord: 1..157
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 131..143
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 3..157

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS014918.1	MS014918.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity