MELO3C020224 (gene) Melon (DHL92) v4

BLAST of MELO3C020224 vs. NCBI nr
Match: XP_008457023.1 (PREDICTED: GDSL esterase/lipase At5g37690 [Cucumis melo])

HSP 1 Score: 753.1 bits (1943), Expect = 1.2e-213
Identity = 375/375 (100.00%), Postives = 375/375 (100.00%), Query Frame = 0

Query: 1   MMRTIRIVGHGFAASMLIGGVMLLFVGFKVCEAKKSSELVTYVFGDSLTEVGNNNYLNSL 60
           MMRTIRIVGHGFAASMLIGGVMLLFVGFKVCEAKKSSELVTYVFGDSLTEVGNNNYLNSL
Sbjct: 1   MMRTIRIVGHGFAASMLIGGVMLLFVGFKVCEAKKSSELVTYVFGDSLTEVGNNNYLNSL 60

Query: 61  ARSDYPWYGVDYNGGQPTGRFTNGRTIGDIISEKLGIEAPPPYLSLSKDDDKLIHGVNYA 120
           ARSDYPWYGVDYNGGQPTGRFTNGRTIGDIISEKLGIEAPPPYLSLSKDDDKLIHGVNYA
Sbjct: 61  ARSDYPWYGVDYNGGQPTGRFTNGRTIGDIISEKLGIEAPPPYLSLSKDDDKLIHGVNYA 120

Query: 121 SGGAGILNDTGLYFIQRLTFDDQIESFNQTKKGIARKIGEEAALKHCNEAIYFIGIGSND 180
           SGGAGILNDTGLYFIQRLTFDDQIESFNQTKKGIARKIGEEAALKHCNEAIYFIGIGSND
Sbjct: 121 SGGAGILNDTGLYFIQRLTFDDQIESFNQTKKGIARKIGEEAALKHCNEAIYFIGIGSND 180

Query: 181 YVNNFLQPFLADGQQYTHEEFLDLLLSTFQQQLTRLYELGARKMVVHGLGPLGCIPSQRV 240
           YVNNFLQPFLADGQQYTHEEFLDLLLSTFQQQLTRLYELGARKMVVHGLGPLGCIPSQRV
Sbjct: 181 YVNNFLQPFLADGQQYTHEEFLDLLLSTFQQQLTRLYELGARKMVVHGLGPLGCIPSQRV 240

Query: 241 KSRKGQCLKRVNQWVQDFNSKVKTLTTTLNKSLPNSHLLFADTYPLVLDLITNPSAYGLK 300
           KSRKGQCLKRVNQWVQDFNSKVKTLTTTLNKSLPNSHLLFADTYPLVLDLITNPSAYGLK
Sbjct: 241 KSRKGQCLKRVNQWVQDFNSKVKTLTTTLNKSLPNSHLLFADTYPLVLDLITNPSAYGLK 300

Query: 301 VSNTSCCNVDTSIGGLCLPNSKICKNRSEYVFWDAFHPSDAANSVLAHQLFSTLFSSSSS 360
           VSNTSCCNVDTSIGGLCLPNSKICKNRSEYVFWDAFHPSDAANSVLAHQLFSTLFSSSSS
Sbjct: 301 VSNTSCCNVDTSIGGLCLPNSKICKNRSEYVFWDAFHPSDAANSVLAHQLFSTLFSSSSS 360

Query: 361 SSSSPSPTPSPFPSP 376
           SSSSPSPTPSPFPSP
Sbjct: 361 SSSSPSPTPSPFPSP 375

BLAST of MELO3C020224 vs. NCBI nr
Match: XP_004145849.1 (GDSL esterase/lipase At5g37690 [Cucumis sativus] >KGN57789.1 hypothetical protein Csa_010024 [Cucumis sativus])

HSP 1 Score: 700.7 bits (1807), Expect = 7.0e-198
Identity = 348/375 (92.80%), Postives = 362/375 (96.53%), Query Frame = 0

Query: 1   MMRTIRIVGHGFAASMLIGGVMLLFVGFKVCEAKKSSELVTYVFGDSLTEVGNNNYLNSL 60
           MMR++  +GHGFAASMLIGGVM+LFVGFKVCEA KSSELVTYVFGDSLTEVGNNN+LNSL
Sbjct: 4   MMRSMITIGHGFAASMLIGGVMVLFVGFKVCEA-KSSELVTYVFGDSLTEVGNNNFLNSL 63

Query: 61  ARSDYPWYGVDYNGGQPTGRFTNGRTIGDIISEKLGIEAPPPYLSLSKDDDKLIHGVNYA 120
           ARSDYPWYGVDYNGGQPTGRFTNGRTIGDIISEKLGIEAPPPYLSL+KDDDKLIHGVNYA
Sbjct: 64  ARSDYPWYGVDYNGGQPTGRFTNGRTIGDIISEKLGIEAPPPYLSLTKDDDKLIHGVNYA 123

Query: 121 SGGAGILNDTGLYFIQRLTFDDQIESFNQTKKGIARKIGEEAALKHCNEAIYFIGIGSND 180
           SGGAGILNDTGLYFIQR+T DDQI+SF QTKK IARKIGEEAAL+HCN+AIYFIGIGSND
Sbjct: 124 SGGAGILNDTGLYFIQRMTLDDQIQSFYQTKKAIARKIGEEAALQHCNQAIYFIGIGSND 183

Query: 181 YVNNFLQPFLADGQQYTHEEFLDLLLSTFQQQLTRLYELGARKMVVHGLGPLGCIPSQRV 240
           YVNNFLQPFLADGQQYTHE+FLDLLLSTFQQQLTRLYELGARKMV+HGLGPLGCIPSQRV
Sbjct: 184 YVNNFLQPFLADGQQYTHEDFLDLLLSTFQQQLTRLYELGARKMVIHGLGPLGCIPSQRV 243

Query: 241 KSRKGQCLKRVNQWVQDFNSKVKTLTTTLNKSLPNSHLLFADTYPLVLDLITNPSAYGLK 300
           KSRKGQCLKRVNQWVQDFNSKVKTLTTTLNK+LPNSHLLFADTYPLVLDLITNPSAYG K
Sbjct: 244 KSRKGQCLKRVNQWVQDFNSKVKTLTTTLNKNLPNSHLLFADTYPLVLDLITNPSAYGFK 303

Query: 301 VSNTSCCNVDTSIGGLCLPNSKICKNRSEYVFWDAFHPSDAANSVLAHQLFSTLFSSSSS 360
           VSNTSCCNVDTSIGGLCLPNSK+CKNRSEYVFWDAFHPSDAANSVLAHQLFSTLFSS SS
Sbjct: 304 VSNTSCCNVDTSIGGLCLPNSKVCKNRSEYVFWDAFHPSDAANSVLAHQLFSTLFSSFSS 363

Query: 361 SSSSPSPTPSPFPSP 376
           SS    PTPSP PSP
Sbjct: 364 SS---PPTPSPSPSP 374

BLAST of MELO3C020224 vs. NCBI nr
Match: XP_038887323.1 (GDSL esterase/lipase At5g37690 [Benincasa hispida])

HSP 1 Score: 625.9 bits (1613), Expect = 2.2e-175
Identity = 314/374 (83.96%), Postives = 341/374 (91.18%), Query Frame = 0

Query: 2   MRTIRIVGHGFAASMLIGGVMLLFVGFKVCEAKKSSELVTYVFGDSLTEVGNNNYLNSLA 61
           M++I I  HGF + ++I  VMLL +GFK CEA  +S+LVTYVFGDSLTEVGNNN+LNSLA
Sbjct: 3   MKSITI--HGFGSIIVIVVVMLL-IGFKPCEA--TSQLVTYVFGDSLTEVGNNNFLNSLA 62

Query: 62  RSDYPWYGVDYNGGQPTGRFTNGRTIGDIISEKLGIEAPPPYLSLSKDDDKLIHGVNYAS 121
           RSDYPWYGVDYNGGQ TGRFTNGRTIGDIIS KLGI +PP YLS++KD+DKL+ GVNYAS
Sbjct: 63  RSDYPWYGVDYNGGQATGRFTNGRTIGDIISSKLGIASPPAYLSVAKDEDKLVQGVNYAS 122

Query: 122 GGAGILNDTGLYFIQRLTFDDQIESFNQTKKGIARKIGEEAALKHCNEAIYFIGIGSNDY 181
           GGAGILNDTGLYFIQRL+FDDQI+SFNQTKK I  K+GEEA  KHCNEA+YFIGIGSNDY
Sbjct: 123 GGAGILNDTGLYFIQRLSFDDQIQSFNQTKKVIENKLGEEATNKHCNEAMYFIGIGSNDY 182

Query: 182 VNNFLQPFLADGQQYTHEEFLDLLLSTFQQQLTRLYELGARKMVVHGLGPLGCIPSQRVK 241
           VNNFLQPFLADGQQYTHEEFLDLLLSTF+QQLTRLY+LGARKMVVHGLGPLGCIPSQRVK
Sbjct: 183 VNNFLQPFLADGQQYTHEEFLDLLLSTFEQQLTRLYQLGARKMVVHGLGPLGCIPSQRVK 242

Query: 242 SRKGQCLKRVNQWVQDFNSKVKTLTTTLNKSLPNSHLLFADTYPLVLDLITNPSAYGLKV 301
           SRKGQCLKRVNQWVQ FNSKV+ LTTTLNK LPNSHLLFADTYPLVLDLI NPS YG KV
Sbjct: 243 SRKGQCLKRVNQWVQHFNSKVQILTTTLNKKLPNSHLLFADTYPLVLDLINNPSVYGFKV 302

Query: 302 SNTSCCNVDTSIGGLCLPNSKICKNRSEYVFWDAFHPSDAANSVLAHQLFSTLFSSSSSS 361
           SNTSCCNVDTSIGGLCLPNSK+CKNRSEYVFWDAFHPSDAAN+V+A++LFSTLF+S  SS
Sbjct: 303 SNTSCCNVDTSIGGLCLPNSKLCKNRSEYVFWDAFHPSDAANAVMANKLFSTLFTSFPSS 362

Query: 362 SSSPSPTPSPFPSP 376
           S   SPTPSP PSP
Sbjct: 363 S---SPTPSPLPSP 368

BLAST of MELO3C020224 vs. NCBI nr
Match: KAG7017931.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 602.8 bits (1553), Expect = 2.0e-168
Identity = 297/366 (81.15%), Postives = 328/366 (89.62%), Query Frame = 0

Query: 10  HGFAASMLIGGVMLLFVGFKVCEAKKSSELVTYVFGDSLTEVGNNNYLNSLARSDYPWYG 69
           H FA+   I    + F G     A+ +S L+TYVFGDSLTEVGNNN+LNSLARSDYPWYG
Sbjct: 7   HAFASIFAI----IFFAG----AAEAASPLITYVFGDSLTEVGNNNFLNSLARSDYPWYG 66

Query: 70  VDYNGGQPTGRFTNGRTIGDIISEKLGIEAPPPYLSLSKDDDKLIHGVNYASGGAGILND 129
           +DYNGGQPTGRFTNG+TIGDIIS KLGI +PPPYLSLS+DDD+L+ GVNYASGGAGILND
Sbjct: 67  LDYNGGQPTGRFTNGQTIGDIISSKLGIPSPPPYLSLSQDDDRLLQGVNYASGGAGILND 126

Query: 130 TGLYFIQRLTFDDQIESFNQTKKGIARKIGEEAALKHCNEAIYFIGIGSNDYVNNFLQPF 189
           TGLYFIQRL+FDDQI++FN+TK  I RKIG+E A  HCNEA+YFIGIGSNDYVNNFLQPF
Sbjct: 127 TGLYFIQRLSFDDQIQNFNKTKMAIRRKIGDEKANIHCNEALYFIGIGSNDYVNNFLQPF 186

Query: 190 LADGQQYTHEEFLDLLLSTFQQQLTRLYELGARKMVVHGLGPLGCIPSQRVKSRKGQCLK 249
           LADGQQYTH+EFLDLLLST +QQ TRLY+LGARKMVVHGLGPLGCIPSQRVKSRKGQCLK
Sbjct: 187 LADGQQYTHDEFLDLLLSTLEQQFTRLYQLGARKMVVHGLGPLGCIPSQRVKSRKGQCLK 246

Query: 250 RVNQWVQDFNSKVKTLTTTLNKSLPNSHLLFADTYPLVLDLITNPSAYGLKVSNTSCCNV 309
           RVNQWVQ FNSK++ L  TLNK+LPN+H LFADTYP+VLDLITNPS YG KVSNTSCCNV
Sbjct: 247 RVNQWVQHFNSKLQILAITLNKTLPNAHFLFADTYPVVLDLITNPSTYGFKVSNTSCCNV 306

Query: 310 DTSIGGLCLPNSKICKNRSEYVFWDAFHPSDAANSVLAHQLFSTLF-SSSSSSSSSPSPT 369
           DTSIGGLCLPNSK+CKNR+EYVFWDAFHPSDAAN+VLAH+LFS+LF SSSSSSSSS SPT
Sbjct: 307 DTSIGGLCLPNSKVCKNRNEYVFWDAFHPSDAANAVLAHKLFSSLFPSSSSSSSSSSSPT 364

Query: 370 PSPFPS 375
           PSP PS
Sbjct: 367 PSPLPS 364

BLAST of MELO3C020224 vs. NCBI nr
Match: XP_022982648.1 (GDSL esterase/lipase At5g37690 [Cucurbita maxima])

HSP 1 Score: 602.4 bits (1552), Expect = 2.6e-168
Identity = 293/361 (81.16%), Postives = 322/361 (89.20%), Query Frame = 0

Query: 10  HGFAASMLIGGVMLLFVGFKVCEAKKSSELVTYVFGDSLTEVGNNNYLNSLARSDYPWYG 69
           H FA+   I     +F       A+ +S LVTYVFGDSLTEVGNNN+LNSLARSDYPWYG
Sbjct: 5   HAFASIFAI-----IFFAGAAGAAEAASPLVTYVFGDSLTEVGNNNFLNSLARSDYPWYG 64

Query: 70  VDYNGGQPTGRFTNGRTIGDIISEKLGIEAPPPYLSLSKDDDKLIHGVNYASGGAGILND 129
           +DYNGGQPTGRFTNGRTIGDIIS KLGI +PPPYLSLS+DDD+L+ GVNYASGGAGILND
Sbjct: 65  LDYNGGQPTGRFTNGRTIGDIISSKLGIPSPPPYLSLSQDDDRLLQGVNYASGGAGILND 124

Query: 130 TGLYFIQRLTFDDQIESFNQTKKGIARKIGEEAALKHCNEAIYFIGIGSNDYVNNFLQPF 189
           TGLYFIQRL+FDDQI++FN+TK  I RKIG+E A KHCNEA+YFIGIGSNDYVNNFLQPF
Sbjct: 125 TGLYFIQRLSFDDQIQNFNKTKMAIRRKIGDEKANKHCNEALYFIGIGSNDYVNNFLQPF 184

Query: 190 LADGQQYTHEEFLDLLLSTFQQQLTRLYELGARKMVVHGLGPLGCIPSQRVKSRKGQCLK 249
           LADGQQYTH+EFLDLLLST +QQ  RLY+LGARKMVVHGLGPLGCIPSQRVKSRKGQCLK
Sbjct: 185 LADGQQYTHDEFLDLLLSTLEQQFKRLYQLGARKMVVHGLGPLGCIPSQRVKSRKGQCLK 244

Query: 250 RVNQWVQDFNSKVKTLTTTLNKSLPNSHLLFADTYPLVLDLITNPSAYGLKVSNTSCCNV 309
           RVNQWVQ FNSK++TL  TLNK+LPN+H LFADTYP+VLDLITNPS YG KVSNTSCCNV
Sbjct: 245 RVNQWVQHFNSKLQTLAITLNKTLPNAHFLFADTYPVVLDLITNPSTYGFKVSNTSCCNV 304

Query: 310 DTSIGGLCLPNSKICKNRSEYVFWDAFHPSDAANSVLAHQLFSTLFSSSSSSSSSPSPTP 369
           DTSIGGLCLPNSK+CKNR EYVFWDAFHPSDAAN+VLAH+LFS+LF SSSSSS +PSP P
Sbjct: 305 DTSIGGLCLPNSKVCKNRREYVFWDAFHPSDAANAVLAHKLFSSLFPSSSSSSPTPSPLP 360

Query: 370 S 371
           S
Sbjct: 365 S 360

BLAST of MELO3C020224 vs. ExPASy Swiss-Prot
Match: Q9FHQ1 (GDSL esterase/lipase At5g37690 OS=Arabidopsis thaliana OX=3702 GN=At5g37690 PE=2 SV=1)

HSP 1 Score: 496.9 bits (1278), Expect = 2.0e-139
Identity = 236/345 (68.41%), Postives = 284/345 (82.32%), Query Frame = 0

Query: 28  FKVCEAKKSSELVTYVFGDSLTEVGNNNYLN-SLARSDYPWYGVDYNGGQPTGRFTNGRT 87
           +   +   +S LVTY+FGDSLTEVGNNN+L  SLAR+D+P+YGVD++GG+ TGRFTNGRT
Sbjct: 15  YATAQPASTSSLVTYIFGDSLTEVGNNNFLQYSLARADFPYYGVDFSGGKATGRFTNGRT 74

Query: 88  IGDIISEKLGIEAPPPYLSLSKDDDKLIHGVNYASGGAGILNDTGLYFIQRLTFDDQIES 147
           IGDIIS KLGI +PPPYLSLS++DD  + G+NYASGGAGILN+TG+YFIQRLTF+DQI  
Sbjct: 75  IGDIISTKLGILSPPPYLSLSQNDDAFLSGINYASGGAGILNETGIYFIQRLTFNDQINC 134

Query: 148 FNQTKKGIARKIGEEAALKHCNEAIYFIGIGSNDYVNNFLQPFLADGQQYTHEEFLDLLL 207
           F +TK+ I  KIG+ AA KH N+A+YFIG+GSNDYVNNFLQPF+ADGQQYTH+EF++LL 
Sbjct: 135 FKKTKEVIRAKIGDGAANKHVNDAMYFIGLGSNDYVNNFLQPFMADGQQYTHDEFVELLT 194

Query: 208 STFQQQLTRLYELGARKMVVHGLGPLGCIPSQRVKSRKGQCLKRVNQWVQDFNSKVKTLT 267
           ST   QLT +Y+LGARK++ HGLGPLGCIPSQRVKS+   CL RVN+WV +FNS+ K L 
Sbjct: 195 STLHNQLTTIYKLGARKVIFHGLGPLGCIPSQRVKSKTRMCLNRVNEWVLEFNSRTKKLL 254

Query: 268 TTLNKSLPNSHLLFADTYPLVLDLITNPSAYGLKVSNTSCCNVDTSIGGLCLPNSKICKN 327
             LNK LP +   FADTYP VLDLI NP+ YG K++NTSCCNVDTS+GGLCLPNSK+CKN
Sbjct: 255 IDLNKRLPGAKFSFADTYPAVLDLINNPTHYGFKIANTSCCNVDTSVGGLCLPNSKMCKN 314

Query: 328 RSEYVFWDAFHPSDAANSVLAHQLFSTLFSSSSSSSSSPSPTPSP 372
           R ++VFWDAFHPSD+AN +LA  LFS+L      SSSSPSP P P
Sbjct: 315 RQDFVFWDAFHPSDSANQILADHLFSSLL-----SSSSPSPAPKP 354

BLAST of MELO3C020224 vs. ExPASy Swiss-Prot
Match: Q9CA68 (GDSL esterase/lipase At1g74460 OS=Arabidopsis thaliana OX=3702 GN=At1g74460 PE=2 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 6.8e-79
Identity = 151/341 (44.28%), Postives = 217/341 (63.64%), Query Frame = 0

Query: 38  ELVTYVFGDSLTEVGNN-NYLNSLARSDYPWYGVDYNGGQPTGRFTNGRTIGDIISEKLG 97
           ++V ++FGDSL++VGNN N   SLA ++ P+YG+D+  G P GRFTNGRT+ DII +K+G
Sbjct: 21  KIVQFIFGDSLSDVGNNKNLPRSLATANLPFYGIDFGNGLPNGRFTNGRTVSDIIGDKIG 80

Query: 98  IEAPPPYLSLSKDDDKLI-HGVNYASGGAGILNDTGLYFIQRLTFDDQIESFNQTKKGIA 157
           +  P  +L  S ++D ++ +GVNYASGG GILN+TG YFIQR +   QIE F  T+  + 
Sbjct: 81  LPRPVAFLDPSMNEDVILENGVNYASGGGGILNETGGYFIQRFSLWKQIELFQGTQDVVV 140

Query: 158 RKIGEEAALKHCNEAIYFIGIGSNDYVNNFLQPFLADGQQYTHEEFLDLLLSTFQQQLTR 217
            KIG++ A K   +A Y + +GSND++NN+L P  +D  +Y  + F+D L+ T + QL  
Sbjct: 141 AKIGKKEADKFFQDARYVVALGSNDFINNYLMPVYSDSWKYNDQTFVDYLMETLESQLKV 200

Query: 218 LYELGARKMVVHGLGPLGCIPSQRVKSRKGQCLKRVNQWVQDFNSKVKTLTTTLNKSLPN 277
           L+ LGARK++V GLGP+GCIP QR  S  G C  + +   + FN    T+   L   LPN
Sbjct: 201 LHSLGARKLMVFGLGPMGCIPLQRALSLDGNCQNKASNLAKRFNKAATTMLLDLETKLPN 260

Query: 278 SHLLFADTYPLVLDLITNPSAYGLKVSNTSCCNVDTSIGGL-CLPNSKICKNRSEYVFWD 337
           +   F + Y LV D+ITNP  YG   S++ CC+       L C+P S +CK+RS+YVFWD
Sbjct: 261 ASYRFGEAYDLVNDVITNPKKYGFDNSDSPCCSFYRIRPALTCIPASTLCKDRSKYVFWD 320

Query: 338 AFHPSDAANSVLAHQLFSTLFSSSSSSSSSPSPTPSPFPSP 376
            +HP+D AN ++A+ L        +    S +P+P+P  SP
Sbjct: 321 EYHPTDKANELVANILIKRFDFMRADDGISHAPSPAPDISP 361

BLAST of MELO3C020224 vs. ExPASy Swiss-Prot
Match: O23470 (GDSL esterase/lipase At4g16230 OS=Arabidopsis thaliana OX=3702 GN=At4g16230 PE=3 SV=2)

HSP 1 Score: 284.3 bits (726), Expect = 2.0e-75
Identity = 149/331 (45.02%), Postives = 214/331 (64.65%), Query Frame = 0

Query: 29  KVCEAKKSSELVTYVFGDSLTEVGNNNYLNSLARSDYPWYGVDYNGGQPTGRFTNGRTIG 88
           +VC A K      +VFGDSL + GNNNYL +L++++Y   G+D+  G PTGRFTNGRTI 
Sbjct: 20  EVCLAGKKIP-ANFVFGDSLVDAGNNNYLATLSKANYVPNGIDF--GSPTGRFTNGRTIV 79

Query: 89  DIISEKLGI-EAPPPYLSLSKDDDKLIHGVNYASGGAGILNDTGLYFIQRLTFDDQIESF 148
           DI+ + LG  E  PPYL+ +     +++GVNYASGG+GILN TG  F +R+  D Q+++F
Sbjct: 80  DIVYQALGSDELTPPYLAPTTSGSLILNGVNYASGGSGILNSTGKLFGERINVDAQLDNF 139

Query: 149 NQTKKGIARKIGEEAALKHCNEAIYFIGIGSNDYVNNFLQPFLADGQQ--YTHEEFLDLL 208
             T++ I   IGE  A K    AI+ +  GSND +NN+  P ++  Q+     E F+D +
Sbjct: 140 ATTRQDIISWIGESEAAKLFRSAIFSVTTGSNDLINNYFTPVISTLQRKVVAPEVFVDTM 199

Query: 209 LSTFQQQLTRLYELGARKMVVHGLGPLGCIPSQRVK--SRKGQCLKRVNQWVQDFNSKVK 268
           +S F+ QLTRLY+LGARK+VV  +GP+GCIP +R    +    CL   N+  Q +N K+K
Sbjct: 200 ISKFRLQLTRLYQLGARKIVVINIGPIGCIPFERESDPAAGNNCLAEPNEVAQMYNLKLK 259

Query: 269 TLTTTLNKSLPNSHLLFADTYPLVLDLITNPSAYGLKVSNTSCCNVDTSIGGL--CLPNS 328
           TL   LNK+L  S  ++ D + +V D+I N S+YG +     CC++   +GGL  C P S
Sbjct: 260 TLVEELNKNLQGSRFVYGDVFRIVDDIIQNYSSYGFESEKIPCCSLVGKVGGLIPCGPPS 319

Query: 329 KICKNRSEYVFWDAFHPSDAANSVLAHQLFS 353
           K+C +RS+YVFWD +HP++AAN ++A +L S
Sbjct: 320 KVCMDRSKYVFWDPYHPTEAANIIIARRLLS 347

BLAST of MELO3C020224 vs. ExPASy Swiss-Prot
Match: Q8LFJ9 (GDSL esterase/lipase 7 OS=Arabidopsis thaliana OX=3702 GN=GLIP7 PE=2 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 1.1e-73
Identity = 147/318 (46.23%), Postives = 202/318 (63.52%), Query Frame = 0

Query: 42  YVFGDSLTEVGNNNYLNSLARSDYPWYGVDYNGGQPTGRFTNGRTIGDIISEKLGIEAPP 101
           +VFGDSL + GNNNY+ +LAR++Y  YG+D+  G PTGRF NGRT+ D  +  LG+   P
Sbjct: 32  FVFGDSLVDSGNNNYIPTLARANYFPYGIDF--GFPTGRFCNGRTVVDYGATYLGLPLVP 91

Query: 102 PYLSLSKDDDKLIHGVNYASGGAGILNDTGLYFIQRLTFDDQIESFNQTKKGIARKIGEE 161
           PYLS        + GVNYAS  AGIL++TG ++  R TF+ QI  F  T +   R+  + 
Sbjct: 92  PYLSPLSIGQNALRGVNYASAAAGILDETGRHYGARTTFNGQISQFEITIELRLRRFFQN 151

Query: 162 AA--LKHCNEAIYFIGIGSNDYVNNFLQP-FLADGQQYTHEEFLDLLLSTFQQQLTRLYE 221
            A   K+  ++I  I IGSNDY+NN+L P   +  Q Y+ E++ DLL+ T   Q++RLY 
Sbjct: 152 PADLRKYLAKSIIGINIGSNDYINNYLMPERYSTSQTYSGEDYADLLIKTLSAQISRLYN 211

Query: 222 LGARKMVVHGLGPLGCIPSQR---VKSRKGQCLKRVNQWVQDFNSKVKTLTTTLNKSLPN 281
           LGARKMV+ G GPLGCIPSQ      +    C+ ++N  V  FNS++K L  TLN +LP 
Sbjct: 212 LGARKMVLAGSGPLGCIPSQLSMVTGNNTSGCVTKINNMVSMFNSRLKDLANTLNTTLPG 271

Query: 282 SHLLFADTYPLVLDLITNPSAYGLKVSNTSCCNVDTSIGGL-CLPNSKICKNRSEYVFWD 341
           S  ++ + + L  D++ NPS YGL VSN +CC      G L CLP  + C +R++YVFWD
Sbjct: 272 SFFVYQNVFDLFHDMVVNPSRYGLVVSNEACCGNGRYGGALTCLPLQQPCLDRNQYVFWD 331

Query: 342 AFHPSDAANSVLAHQLFS 353
           AFHP++ AN ++AH  FS
Sbjct: 332 AFHPTETANKIIAHNTFS 347

BLAST of MELO3C020224 vs. ExPASy Swiss-Prot
Match: Q9SF78 (GDSL esterase/lipase At1g71691 OS=Arabidopsis thaliana OX=3702 GN=At1g71691 PE=2 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 3.3e-73
Identity = 140/313 (44.73%), Postives = 199/313 (63.58%), Query Frame = 0

Query: 42  YVFGDSLTEVGNNNYLNSLARSDYPWYGVDYNGGQPTGRFTNGRTIGDIISEKLGIEAPP 101
           +VFGDSL + GNNN + S A+++Y  YG+D+NGG PTGRF NG T+ D I++ LG+   P
Sbjct: 57  FVFGDSLIDNGNNNNIPSFAKANYFPYGIDFNGG-PTGRFCNGLTMVDGIAQLLGLPLIP 116

Query: 102 PYLSLSKDDDKLIHGVNYASGGAGILNDTGLYFIQRLTFDDQIESFNQTKKGIARKIGEE 161
            Y   +   D+++ GVNYAS  AGIL DTG  F+ R+ FD QI +F  T   +A K G  
Sbjct: 117 AYSEAT--GDQVLRGVNYASAAAGILPDTGGNFVGRIPFDQQIHNFETTLDQVASKSGGA 176

Query: 162 AAL-KHCNEAIYFIGIGSNDYVNNFLQPFLADGQQYTHEEFLDLLLSTFQQQLTRLYELG 221
            A+      +++FIG+GSNDY+NN+L P      QY  ++F DLL+  +  QLTRLY LG
Sbjct: 177 VAIADSVTRSLFFIGMGSNDYLNNYLMPNFPTRNQYNSQQFGDLLVQHYTDQLTRLYNLG 236

Query: 222 ARKMVVHGLGPLGCIPSQRVKSRKGQCLKRVNQWVQDFNSKVKTLTTTLNKSLPNSHLLF 281
            RK VV GLG +GCIPS   +   G+C + VNQ V  FN+ VKT+ + LN++LP++  ++
Sbjct: 237 GRKFVVAGLGRMGCIPSILAQGNDGKCSEEVNQLVLPFNTNVKTMISNLNQNLPDAKFIY 296

Query: 282 ADTYPLVLDLITNPSAYGLKVSNTSCCNVDTSIGGL-CLPNSKICKNRSEYVFWDAFHPS 341
            D   +  D++ N +AYGL   +  CC +  + G + CLP    C NR +YVFWDAFHP+
Sbjct: 297 LDIAHMFEDIVANQAAYGLTTMDKGCCGIGKNRGQITCLPFETPCPNRDQYVFWDAFHPT 356

Query: 342 DAANSVLAHQLFS 353
           +  N ++A + F+
Sbjct: 357 EKVNLIMAKKAFA 366

BLAST of MELO3C020224 vs. ExPASy TrEMBL
Match: A0A1S3C4J0 (GDSL esterase/lipase At5g37690 OS=Cucumis melo OX=3656 GN=LOC103496804 PE=3 SV=1)

HSP 1 Score: 753.1 bits (1943), Expect = 5.8e-214
Identity = 375/375 (100.00%), Postives = 375/375 (100.00%), Query Frame = 0

Query: 1   MMRTIRIVGHGFAASMLIGGVMLLFVGFKVCEAKKSSELVTYVFGDSLTEVGNNNYLNSL 60
           MMRTIRIVGHGFAASMLIGGVMLLFVGFKVCEAKKSSELVTYVFGDSLTEVGNNNYLNSL
Sbjct: 1   MMRTIRIVGHGFAASMLIGGVMLLFVGFKVCEAKKSSELVTYVFGDSLTEVGNNNYLNSL 60

Query: 61  ARSDYPWYGVDYNGGQPTGRFTNGRTIGDIISEKLGIEAPPPYLSLSKDDDKLIHGVNYA 120
           ARSDYPWYGVDYNGGQPTGRFTNGRTIGDIISEKLGIEAPPPYLSLSKDDDKLIHGVNYA
Sbjct: 61  ARSDYPWYGVDYNGGQPTGRFTNGRTIGDIISEKLGIEAPPPYLSLSKDDDKLIHGVNYA 120

Query: 121 SGGAGILNDTGLYFIQRLTFDDQIESFNQTKKGIARKIGEEAALKHCNEAIYFIGIGSND 180
           SGGAGILNDTGLYFIQRLTFDDQIESFNQTKKGIARKIGEEAALKHCNEAIYFIGIGSND
Sbjct: 121 SGGAGILNDTGLYFIQRLTFDDQIESFNQTKKGIARKIGEEAALKHCNEAIYFIGIGSND 180

Query: 181 YVNNFLQPFLADGQQYTHEEFLDLLLSTFQQQLTRLYELGARKMVVHGLGPLGCIPSQRV 240
           YVNNFLQPFLADGQQYTHEEFLDLLLSTFQQQLTRLYELGARKMVVHGLGPLGCIPSQRV
Sbjct: 181 YVNNFLQPFLADGQQYTHEEFLDLLLSTFQQQLTRLYELGARKMVVHGLGPLGCIPSQRV 240

Query: 241 KSRKGQCLKRVNQWVQDFNSKVKTLTTTLNKSLPNSHLLFADTYPLVLDLITNPSAYGLK 300
           KSRKGQCLKRVNQWVQDFNSKVKTLTTTLNKSLPNSHLLFADTYPLVLDLITNPSAYGLK
Sbjct: 241 KSRKGQCLKRVNQWVQDFNSKVKTLTTTLNKSLPNSHLLFADTYPLVLDLITNPSAYGLK 300

Query: 301 VSNTSCCNVDTSIGGLCLPNSKICKNRSEYVFWDAFHPSDAANSVLAHQLFSTLFSSSSS 360
           VSNTSCCNVDTSIGGLCLPNSKICKNRSEYVFWDAFHPSDAANSVLAHQLFSTLFSSSSS
Sbjct: 301 VSNTSCCNVDTSIGGLCLPNSKICKNRSEYVFWDAFHPSDAANSVLAHQLFSTLFSSSSS 360

Query: 361 SSSSPSPTPSPFPSP 376
           SSSSPSPTPSPFPSP
Sbjct: 361 SSSSPSPTPSPFPSP 375

BLAST of MELO3C020224 vs. ExPASy TrEMBL
Match: A0A0A0L9Y6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G303130 PE=3 SV=1)

HSP 1 Score: 700.7 bits (1807), Expect = 3.4e-198
Identity = 348/375 (92.80%), Postives = 362/375 (96.53%), Query Frame = 0

Query: 1   MMRTIRIVGHGFAASMLIGGVMLLFVGFKVCEAKKSSELVTYVFGDSLTEVGNNNYLNSL 60
           MMR++  +GHGFAASMLIGGVM+LFVGFKVCEA KSSELVTYVFGDSLTEVGNNN+LNSL
Sbjct: 4   MMRSMITIGHGFAASMLIGGVMVLFVGFKVCEA-KSSELVTYVFGDSLTEVGNNNFLNSL 63

Query: 61  ARSDYPWYGVDYNGGQPTGRFTNGRTIGDIISEKLGIEAPPPYLSLSKDDDKLIHGVNYA 120
           ARSDYPWYGVDYNGGQPTGRFTNGRTIGDIISEKLGIEAPPPYLSL+KDDDKLIHGVNYA
Sbjct: 64  ARSDYPWYGVDYNGGQPTGRFTNGRTIGDIISEKLGIEAPPPYLSLTKDDDKLIHGVNYA 123

Query: 121 SGGAGILNDTGLYFIQRLTFDDQIESFNQTKKGIARKIGEEAALKHCNEAIYFIGIGSND 180
           SGGAGILNDTGLYFIQR+T DDQI+SF QTKK IARKIGEEAAL+HCN+AIYFIGIGSND
Sbjct: 124 SGGAGILNDTGLYFIQRMTLDDQIQSFYQTKKAIARKIGEEAALQHCNQAIYFIGIGSND 183

Query: 181 YVNNFLQPFLADGQQYTHEEFLDLLLSTFQQQLTRLYELGARKMVVHGLGPLGCIPSQRV 240
           YVNNFLQPFLADGQQYTHE+FLDLLLSTFQQQLTRLYELGARKMV+HGLGPLGCIPSQRV
Sbjct: 184 YVNNFLQPFLADGQQYTHEDFLDLLLSTFQQQLTRLYELGARKMVIHGLGPLGCIPSQRV 243

Query: 241 KSRKGQCLKRVNQWVQDFNSKVKTLTTTLNKSLPNSHLLFADTYPLVLDLITNPSAYGLK 300
           KSRKGQCLKRVNQWVQDFNSKVKTLTTTLNK+LPNSHLLFADTYPLVLDLITNPSAYG K
Sbjct: 244 KSRKGQCLKRVNQWVQDFNSKVKTLTTTLNKNLPNSHLLFADTYPLVLDLITNPSAYGFK 303

Query: 301 VSNTSCCNVDTSIGGLCLPNSKICKNRSEYVFWDAFHPSDAANSVLAHQLFSTLFSSSSS 360
           VSNTSCCNVDTSIGGLCLPNSK+CKNRSEYVFWDAFHPSDAANSVLAHQLFSTLFSS SS
Sbjct: 304 VSNTSCCNVDTSIGGLCLPNSKVCKNRSEYVFWDAFHPSDAANSVLAHQLFSTLFSSFSS 363

Query: 361 SSSSPSPTPSPFPSP 376
           SS    PTPSP PSP
Sbjct: 364 SS---PPTPSPSPSP 374

BLAST of MELO3C020224 vs. ExPASy TrEMBL
Match: A0A6J1J5D8 (GDSL esterase/lipase At5g37690 OS=Cucurbita maxima OX=3661 GN=LOC111481462 PE=3 SV=1)

HSP 1 Score: 602.4 bits (1552), Expect = 1.3e-168
Identity = 293/361 (81.16%), Postives = 322/361 (89.20%), Query Frame = 0

Query: 10  HGFAASMLIGGVMLLFVGFKVCEAKKSSELVTYVFGDSLTEVGNNNYLNSLARSDYPWYG 69
           H FA+   I     +F       A+ +S LVTYVFGDSLTEVGNNN+LNSLARSDYPWYG
Sbjct: 5   HAFASIFAI-----IFFAGAAGAAEAASPLVTYVFGDSLTEVGNNNFLNSLARSDYPWYG 64

Query: 70  VDYNGGQPTGRFTNGRTIGDIISEKLGIEAPPPYLSLSKDDDKLIHGVNYASGGAGILND 129
           +DYNGGQPTGRFTNGRTIGDIIS KLGI +PPPYLSLS+DDD+L+ GVNYASGGAGILND
Sbjct: 65  LDYNGGQPTGRFTNGRTIGDIISSKLGIPSPPPYLSLSQDDDRLLQGVNYASGGAGILND 124

Query: 130 TGLYFIQRLTFDDQIESFNQTKKGIARKIGEEAALKHCNEAIYFIGIGSNDYVNNFLQPF 189
           TGLYFIQRL+FDDQI++FN+TK  I RKIG+E A KHCNEA+YFIGIGSNDYVNNFLQPF
Sbjct: 125 TGLYFIQRLSFDDQIQNFNKTKMAIRRKIGDEKANKHCNEALYFIGIGSNDYVNNFLQPF 184

Query: 190 LADGQQYTHEEFLDLLLSTFQQQLTRLYELGARKMVVHGLGPLGCIPSQRVKSRKGQCLK 249
           LADGQQYTH+EFLDLLLST +QQ  RLY+LGARKMVVHGLGPLGCIPSQRVKSRKGQCLK
Sbjct: 185 LADGQQYTHDEFLDLLLSTLEQQFKRLYQLGARKMVVHGLGPLGCIPSQRVKSRKGQCLK 244

Query: 250 RVNQWVQDFNSKVKTLTTTLNKSLPNSHLLFADTYPLVLDLITNPSAYGLKVSNTSCCNV 309
           RVNQWVQ FNSK++TL  TLNK+LPN+H LFADTYP+VLDLITNPS YG KVSNTSCCNV
Sbjct: 245 RVNQWVQHFNSKLQTLAITLNKTLPNAHFLFADTYPVVLDLITNPSTYGFKVSNTSCCNV 304

Query: 310 DTSIGGLCLPNSKICKNRSEYVFWDAFHPSDAANSVLAHQLFSTLFSSSSSSSSSPSPTP 369
           DTSIGGLCLPNSK+CKNR EYVFWDAFHPSDAAN+VLAH+LFS+LF SSSSSS +PSP P
Sbjct: 305 DTSIGGLCLPNSKVCKNRREYVFWDAFHPSDAANAVLAHKLFSSLFPSSSSSSPTPSPLP 360

Query: 370 S 371
           S
Sbjct: 365 S 360

BLAST of MELO3C020224 vs. ExPASy TrEMBL
Match: A0A6J1F2C7 (GDSL esterase/lipase At5g37690 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111441552 PE=3 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 2.8e-168
Identity = 297/365 (81.37%), Postives = 326/365 (89.32%), Query Frame = 0

Query: 10  HGFAASMLIGGVMLLFVGFKVCEAKKSSELVTYVFGDSLTEVGNNNYLNSLARSDYPWYG 69
           H FA+   I    + F G     A+ +S LVTYVFGDSLTEVGNNN+LNSLARSDYPWYG
Sbjct: 5   HAFASIFAI----IFFAG----AAEAASPLVTYVFGDSLTEVGNNNFLNSLARSDYPWYG 64

Query: 70  VDYNGGQPTGRFTNGRTIGDIISEKLGIEAPPPYLSLSKDDDKLIHGVNYASGGAGILND 129
           +DYNGGQPTGRFTNGRTIGDIIS KLGI +PPPYLSLS+DDD+L+ GVNYASGGAGILND
Sbjct: 65  LDYNGGQPTGRFTNGRTIGDIISSKLGIPSPPPYLSLSQDDDRLLQGVNYASGGAGILND 124

Query: 130 TGLYFIQRLTFDDQIESFNQTKKGIARKIGEEAALKHCNEAIYFIGIGSNDYVNNFLQPF 189
           TGLYFIQRL+FDDQI++FN+TK  I RKIG+E A  HCNEA+YFIGIGSNDYVNNFLQPF
Sbjct: 125 TGLYFIQRLSFDDQIQNFNRTKMAIRRKIGDEKANIHCNEALYFIGIGSNDYVNNFLQPF 184

Query: 190 LADGQQYTHEEFLDLLLSTFQQQLTRLYELGARKMVVHGLGPLGCIPSQRVKSRKGQCLK 249
           LADGQQYTH+EFL LLLST +QQ TRLY+LGARKMVVHGLGPLGCIPSQRVKSRKGQCLK
Sbjct: 185 LADGQQYTHDEFLSLLLSTLEQQFTRLYQLGARKMVVHGLGPLGCIPSQRVKSRKGQCLK 244

Query: 250 RVNQWVQDFNSKVKTLTTTLNKSLPNSHLLFADTYPLVLDLITNPSAYGLKVSNTSCCNV 309
           RVNQWVQ FNSK++ L  TLNK+LPN+H LFADTYP+VLDLITNPS YG KVSNTSCCNV
Sbjct: 245 RVNQWVQHFNSKLQILAITLNKTLPNAHFLFADTYPVVLDLITNPSTYGFKVSNTSCCNV 304

Query: 310 DTSIGGLCLPNSKICKNRSEYVFWDAFHPSDAANSVLAHQLFSTLFSSSSSSSSSPSPTP 369
           DTSIGGLCLPNSK+CKNR+EYVFWDAFHPSDAAN+VLAH+LFS+LF SSSSSSSS SPTP
Sbjct: 305 DTSIGGLCLPNSKVCKNRNEYVFWDAFHPSDAANAVLAHKLFSSLFPSSSSSSSS-SPTP 360

Query: 370 SPFPS 375
           SP PS
Sbjct: 365 SPLPS 360

BLAST of MELO3C020224 vs. ExPASy TrEMBL
Match: A0A6J1CQN2 (GDSL esterase/lipase At5g37690 OS=Momordica charantia OX=3673 GN=LOC111013293 PE=3 SV=1)

HSP 1 Score: 577.4 bits (1487), Expect = 4.3e-161
Identity = 281/354 (79.38%), Postives = 318/354 (89.83%), Query Frame = 0

Query: 12  FAASMLIGGVMLLFVGFKVCEAKKSSELVTYVFGDSLTEVGNNNYLNSLARSDYPWYGVD 71
           F +S ++  V+L+F GF    ++++S +VTYVFGDSLT+VGNNNYLNSLARS+YPWYGVD
Sbjct: 10  FVSSFMV-VVILMFFGF----SEEASHVVTYVFGDSLTDVGNNNYLNSLARSNYPWYGVD 69

Query: 72  YNGGQPTGRFTNGRTIGDIISEKLGIEAPPPYLSLSKDDDKLIHGVNYASGGAGILNDTG 131
           YNGG+ TGRF+NGRTIGDIIS KLGI  PPPYLSLS+DDD+L+ GVNYASGGAGILNDTG
Sbjct: 70  YNGGRATGRFSNGRTIGDIISSKLGIPPPPPYLSLSQDDDRLLQGVNYASGGAGILNDTG 129

Query: 132 LYFIQRLTFDDQIESFNQTKKGIARKIGEEAALKHCNEAIYFIGIGSNDYVNNFLQPFLA 191
           LYFIQRL+FDDQI+SFN+TK+ I  KIGEEAA KHCNEA+YFIGIGSNDYVNNFLQPFLA
Sbjct: 130 LYFIQRLSFDDQIQSFNKTKRAIRGKIGEEAAKKHCNEAMYFIGIGSNDYVNNFLQPFLA 189

Query: 192 DGQQYTHEEFLDLLLSTFQQQLTRLYELGARKMVVHGLGPLGCIPSQRVKSRKGQCLKRV 251
           DGQQYTH+EFL+LL+ST   QL RLY+LGARKMV+HGLGPLGCIPSQRVKSRKGQCLKRV
Sbjct: 190 DGQQYTHDEFLELLVSTLDHQLLRLYQLGARKMVLHGLGPLGCIPSQRVKSRKGQCLKRV 249

Query: 252 NQWVQDFNSKVKTLTTTLNKSLPNSHLLFADTYPLVLDLITNPSAYGLKVSNTSCCNVDT 311
           NQWVQ+FNSKV+TLT TLNK LPN+ LLFADTY  VLDLITNP AYG KVSNTSCCNVDT
Sbjct: 250 NQWVQEFNSKVQTLTATLNKHLPNAQLLFADTYSTVLDLITNPFAYGFKVSNTSCCNVDT 309

Query: 312 SIGGLCLPNSKICKNRSEYVFWDAFHPSDAANSVLAHQLFSTLFSSSSSSSSSP 366
           SIGGLCLPNSK+C NRSEYVFWDAFHPSDAAN++LA +LF+TLF+S S S + P
Sbjct: 310 SIGGLCLPNSKLCSNRSEYVFWDAFHPSDAANAILADKLFATLFTSPSPSPALP 358

BLAST of MELO3C020224 vs. TAIR 10
Match: AT5G37690.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 496.9 bits (1278), Expect = 1.4e-140
Identity = 236/345 (68.41%), Postives = 284/345 (82.32%), Query Frame = 0

Query: 28  FKVCEAKKSSELVTYVFGDSLTEVGNNNYLN-SLARSDYPWYGVDYNGGQPTGRFTNGRT 87
           +   +   +S LVTY+FGDSLTEVGNNN+L  SLAR+D+P+YGVD++GG+ TGRFTNGRT
Sbjct: 15  YATAQPASTSSLVTYIFGDSLTEVGNNNFLQYSLARADFPYYGVDFSGGKATGRFTNGRT 74

Query: 88  IGDIISEKLGIEAPPPYLSLSKDDDKLIHGVNYASGGAGILNDTGLYFIQRLTFDDQIES 147
           IGDIIS KLGI +PPPYLSLS++DD  + G+NYASGGAGILN+TG+YFIQRLTF+DQI  
Sbjct: 75  IGDIISTKLGILSPPPYLSLSQNDDAFLSGINYASGGAGILNETGIYFIQRLTFNDQINC 134

Query: 148 FNQTKKGIARKIGEEAALKHCNEAIYFIGIGSNDYVNNFLQPFLADGQQYTHEEFLDLLL 207
           F +TK+ I  KIG+ AA KH N+A+YFIG+GSNDYVNNFLQPF+ADGQQYTH+EF++LL 
Sbjct: 135 FKKTKEVIRAKIGDGAANKHVNDAMYFIGLGSNDYVNNFLQPFMADGQQYTHDEFVELLT 194

Query: 208 STFQQQLTRLYELGARKMVVHGLGPLGCIPSQRVKSRKGQCLKRVNQWVQDFNSKVKTLT 267
           ST   QLT +Y+LGARK++ HGLGPLGCIPSQRVKS+   CL RVN+WV +FNS+ K L 
Sbjct: 195 STLHNQLTTIYKLGARKVIFHGLGPLGCIPSQRVKSKTRMCLNRVNEWVLEFNSRTKKLL 254

Query: 268 TTLNKSLPNSHLLFADTYPLVLDLITNPSAYGLKVSNTSCCNVDTSIGGLCLPNSKICKN 327
             LNK LP +   FADTYP VLDLI NP+ YG K++NTSCCNVDTS+GGLCLPNSK+CKN
Sbjct: 255 IDLNKRLPGAKFSFADTYPAVLDLINNPTHYGFKIANTSCCNVDTSVGGLCLPNSKMCKN 314

Query: 328 RSEYVFWDAFHPSDAANSVLAHQLFSTLFSSSSSSSSSPSPTPSP 372
           R ++VFWDAFHPSD+AN +LA  LFS+L      SSSSPSP P P
Sbjct: 315 RQDFVFWDAFHPSDSANQILADHLFSSLL-----SSSSPSPAPKP 354

BLAST of MELO3C020224 vs. TAIR 10
Match: AT1G74460.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 295.8 bits (756), Expect = 4.8e-80
Identity = 151/341 (44.28%), Postives = 217/341 (63.64%), Query Frame = 0

Query: 38  ELVTYVFGDSLTEVGNN-NYLNSLARSDYPWYGVDYNGGQPTGRFTNGRTIGDIISEKLG 97
           ++V ++FGDSL++VGNN N   SLA ++ P+YG+D+  G P GRFTNGRT+ DII +K+G
Sbjct: 21  KIVQFIFGDSLSDVGNNKNLPRSLATANLPFYGIDFGNGLPNGRFTNGRTVSDIIGDKIG 80

Query: 98  IEAPPPYLSLSKDDDKLI-HGVNYASGGAGILNDTGLYFIQRLTFDDQIESFNQTKKGIA 157
           +  P  +L  S ++D ++ +GVNYASGG GILN+TG YFIQR +   QIE F  T+  + 
Sbjct: 81  LPRPVAFLDPSMNEDVILENGVNYASGGGGILNETGGYFIQRFSLWKQIELFQGTQDVVV 140

Query: 158 RKIGEEAALKHCNEAIYFIGIGSNDYVNNFLQPFLADGQQYTHEEFLDLLLSTFQQQLTR 217
            KIG++ A K   +A Y + +GSND++NN+L P  +D  +Y  + F+D L+ T + QL  
Sbjct: 141 AKIGKKEADKFFQDARYVVALGSNDFINNYLMPVYSDSWKYNDQTFVDYLMETLESQLKV 200

Query: 218 LYELGARKMVVHGLGPLGCIPSQRVKSRKGQCLKRVNQWVQDFNSKVKTLTTTLNKSLPN 277
           L+ LGARK++V GLGP+GCIP QR  S  G C  + +   + FN    T+   L   LPN
Sbjct: 201 LHSLGARKLMVFGLGPMGCIPLQRALSLDGNCQNKASNLAKRFNKAATTMLLDLETKLPN 260

Query: 278 SHLLFADTYPLVLDLITNPSAYGLKVSNTSCCNVDTSIGGL-CLPNSKICKNRSEYVFWD 337
           +   F + Y LV D+ITNP  YG   S++ CC+       L C+P S +CK+RS+YVFWD
Sbjct: 261 ASYRFGEAYDLVNDVITNPKKYGFDNSDSPCCSFYRIRPALTCIPASTLCKDRSKYVFWD 320

Query: 338 AFHPSDAANSVLAHQLFSTLFSSSSSSSSSPSPTPSPFPSP 376
            +HP+D AN ++A+ L        +    S +P+P+P  SP
Sbjct: 321 EYHPTDKANELVANILIKRFDFMRADDGISHAPSPAPDISP 361

BLAST of MELO3C020224 vs. TAIR 10
Match: AT5G15720.1 (GDSL-motif lipase 7 )

HSP 1 Score: 278.5 bits (711), Expect = 8.0e-75
Identity = 147/318 (46.23%), Postives = 202/318 (63.52%), Query Frame = 0

Query: 42  YVFGDSLTEVGNNNYLNSLARSDYPWYGVDYNGGQPTGRFTNGRTIGDIISEKLGIEAPP 101
           +VFGDSL + GNNNY+ +LAR++Y  YG+D+  G PTGRF NGRT+ D  +  LG+   P
Sbjct: 32  FVFGDSLVDSGNNNYIPTLARANYFPYGIDF--GFPTGRFCNGRTVVDYGATYLGLPLVP 91

Query: 102 PYLSLSKDDDKLIHGVNYASGGAGILNDTGLYFIQRLTFDDQIESFNQTKKGIARKIGEE 161
           PYLS        + GVNYAS  AGIL++TG ++  R TF+ QI  F  T +   R+  + 
Sbjct: 92  PYLSPLSIGQNALRGVNYASAAAGILDETGRHYGARTTFNGQISQFEITIELRLRRFFQN 151

Query: 162 AA--LKHCNEAIYFIGIGSNDYVNNFLQP-FLADGQQYTHEEFLDLLLSTFQQQLTRLYE 221
            A   K+  ++I  I IGSNDY+NN+L P   +  Q Y+ E++ DLL+ T   Q++RLY 
Sbjct: 152 PADLRKYLAKSIIGINIGSNDYINNYLMPERYSTSQTYSGEDYADLLIKTLSAQISRLYN 211

Query: 222 LGARKMVVHGLGPLGCIPSQR---VKSRKGQCLKRVNQWVQDFNSKVKTLTTTLNKSLPN 281
           LGARKMV+ G GPLGCIPSQ      +    C+ ++N  V  FNS++K L  TLN +LP 
Sbjct: 212 LGARKMVLAGSGPLGCIPSQLSMVTGNNTSGCVTKINNMVSMFNSRLKDLANTLNTTLPG 271

Query: 282 SHLLFADTYPLVLDLITNPSAYGLKVSNTSCCNVDTSIGGL-CLPNSKICKNRSEYVFWD 341
           S  ++ + + L  D++ NPS YGL VSN +CC      G L CLP  + C +R++YVFWD
Sbjct: 272 SFFVYQNVFDLFHDMVVNPSRYGLVVSNEACCGNGRYGGALTCLPLQQPCLDRNQYVFWD 331

Query: 342 AFHPSDAANSVLAHQLFS 353
           AFHP++ AN ++AH  FS
Sbjct: 332 AFHPTETANKIIAHNTFS 347

BLAST of MELO3C020224 vs. TAIR 10
Match: AT1G71691.2 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 276.9 bits (707), Expect = 2.3e-74
Identity = 140/313 (44.73%), Postives = 199/313 (63.58%), Query Frame = 0

Query: 42  YVFGDSLTEVGNNNYLNSLARSDYPWYGVDYNGGQPTGRFTNGRTIGDIISEKLGIEAPP 101
           +VFGDSL + GNNN + S A+++Y  YG+D+NGG PTGRF NG T+ D I++ LG+   P
Sbjct: 57  FVFGDSLIDNGNNNNIPSFAKANYFPYGIDFNGG-PTGRFCNGLTMVDGIAQLLGLPLIP 116

Query: 102 PYLSLSKDDDKLIHGVNYASGGAGILNDTGLYFIQRLTFDDQIESFNQTKKGIARKIGEE 161
            Y   +   D+++ GVNYAS  AGIL DTG  F+ R+ FD QI +F  T   +A K G  
Sbjct: 117 AYSEAT--GDQVLRGVNYASAAAGILPDTGGNFVGRIPFDQQIHNFETTLDQVASKSGGA 176

Query: 162 AAL-KHCNEAIYFIGIGSNDYVNNFLQPFLADGQQYTHEEFLDLLLSTFQQQLTRLYELG 221
            A+      +++FIG+GSNDY+NN+L P      QY  ++F DLL+  +  QLTRLY LG
Sbjct: 177 VAIADSVTRSLFFIGMGSNDYLNNYLMPNFPTRNQYNSQQFGDLLVQHYTDQLTRLYNLG 236

Query: 222 ARKMVVHGLGPLGCIPSQRVKSRKGQCLKRVNQWVQDFNSKVKTLTTTLNKSLPNSHLLF 281
            RK VV GLG +GCIPS   +   G+C + VNQ V  FN+ VKT+ + LN++LP++  ++
Sbjct: 237 GRKFVVAGLGRMGCIPSILAQGNDGKCSEEVNQLVLPFNTNVKTMISNLNQNLPDAKFIY 296

Query: 282 ADTYPLVLDLITNPSAYGLKVSNTSCCNVDTSIGGL-CLPNSKICKNRSEYVFWDAFHPS 341
            D   +  D++ N +AYGL   +  CC +  + G + CLP    C NR +YVFWDAFHP+
Sbjct: 297 LDIAHMFEDIVANQAAYGLTTMDKGCCGIGKNRGQITCLPFETPCPNRDQYVFWDAFHPT 356

Query: 342 DAANSVLAHQLFS 353
           +  N ++A + F+
Sbjct: 357 EKVNLIMAKKAFA 366

BLAST of MELO3C020224 vs. TAIR 10
Match: AT2G23540.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 272.3 bits (695), Expect = 5.7e-73
Identity = 138/320 (43.12%), Postives = 208/320 (65.00%), Query Frame = 0

Query: 41  TYVFGDSLTEVGNNNYLNSLARSDYPWYGVDY--NGGQPTGRFTNGRTIGDIISEKLG-I 100
           +++FGDSL + GNNNYL++L+R++    G+D+  +GG PTGRFTNGRTIGDI+ E+LG  
Sbjct: 49  SFIFGDSLVDAGNNNYLSTLSRANMKPNGIDFKASGGTPTGRFTNGRTIGDIVGEELGSA 108

Query: 101 EAPPPYLSLSKDDDKLIHGVNYASGGAGILNDTGLYFIQRLTFDDQIESFNQTKKGIARK 160
               P+L+       L+ GVNYASGG GI+N TG  F+ RL  D Q++ FN T+K     
Sbjct: 109 NYAIPFLAPDAKGKALLAGVNYASGGGGIMNATGRIFVNRLGMDVQVDFFNTTRKQFDDL 168

Query: 161 IGEEAALKH-CNEAIYFIGIGSNDYVNNFLQPFLADGQQYTH--EEFLDLLLSTFQQQLT 220
           +G+E A  +   ++I+ I IG+ND++NN+L P L+ G ++T   ++F+  +L   + QLT
Sbjct: 169 LGKEKAKDYIAKKSIFSITIGANDFLNNYLFPLLSVGTRFTQTPDDFIGDMLEHLRDQLT 228

Query: 221 RLYELGARKMVVHGLGPLGCIPSQRVKSR--KGQCLKRVNQWVQDFNSKVKTLTTTLNKS 280
           RLY+L ARK V+  +GP+GCIP Q+  ++  + +C+   N+    +N ++K+L   LNK 
Sbjct: 229 RLYQLDARKFVIGNVGPIGCIPYQKTINQLDENECVDLANKLANQYNVRLKSLLEELNKK 288

Query: 281 LPNSHLLFADTYPLVLDLITNPSAYGLKVSNTSCCNVDTSIGGL--CLPNSKICKNRSEY 340
           LP +  + A+ Y LV++LITN   YG K +  +CC       G+  C P S +C+ R +Y
Sbjct: 289 LPGAMFVHANVYDLVMELITNYDKYGFKSATKACCGNGGQYAGIIPCGPTSSLCEERDKY 348

Query: 341 VFWDAFHPSDAANSVLAHQL 351
           VFWD +HPS+AAN ++A QL
Sbjct: 349 VFWDPYHPSEAANVIIAKQL 368