MC03g0153 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC03g0153
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	RNase H domain-containing protein
Location	MC03: 2673931 .. 2674337 (-)
RNA-Seq Expression	MC03g0153
Synteny	MC03g0153

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TGGTCAATGCCTCCAAAAGGGGTCCTTAAATGTAACACTAATGCCACGTGTGACTCTAGAAGAATGAAATCAGGGATCGGGGTTGTCTTTCGAGATGATCAAAATTTCCTTCAGGCGGCGATGCTAGCAACTTTCCCCGAAATATCCCCTCTCTCGGCTAAAGTGCTCGCGACTCGGGAGGCAGTTAGGCTGGCAGCAAGGTTGGGGTGCCTCGGGTGTTGTTTGAATCAGATTCTTTAATGGTAATACAATTGATTCGAGGCGATTTAGAGTGGCAAGGAGAAATTGAAACTTTGGTTCTTAACATTAGGGCTTTGGCTTCTTCGTTCTCTTCGTTTTCCTTCTTGCATGTGCCAAGGGAAGCGAATGGAGTGGCTCACCTGGTGGCTAAGAGGTCTTTGGAGTGG

mRNA sequence

TGGTCAATGCCTCCAAAAGGGGTCCTTAAATGTAACACTAATGCCACGTGTGACTCTAGAAGAATGAAATCAGGGATCGGGGTTGTCTTTCGAGATGATCAAAATTTCCTTCAGGCGGCGATGCTAGCAACTTTCCCCGAAATATCCCCTCTCTCGGCTAAAGTGCTCGCGACTCGGGAGGCAGTTAGGCTGGCAGCAAGGTTGGTGCCTCGGGTGTTGTTTGAATCAGATTCTTTAATGGTAATACAATTGATTCGAGGCGATTTAGAGTGGCAAGGAGAAATTGAAACTTTGGTTCTTAACATTAGGGCTTTGGCTTCTTCGTTCTCTTCGTTTTCCTTCTTGCATGTGCCAAGGGAAGCGAATGGAGTGGCTCACCTGGTGGCTAAGAGGTCTTTGGAGTGG

Coding sequence (CDS)

TGGTCAATGCCTCCAAAAGGGGTCCTTAAATGTAACACTAATGCCACGTGTGACTCTAGAAGAATGAAATCAGGGATCGGGGTTGTCTTTCGAGATGATCAAAATTTCCTTCAGGCGGCGATGCTAGCAACTTTCCCCGAAATATCCCCTCTCTCGGCTAAAGTGCTCGCGACTCGGGAGGCAGTTAGGCTGGCAGCAAGGTTGGTGCCTCGGGTGTTGTTTGAATCAGATTCTTTAATGGTAATACAATTGATTCGAGGCGATTTAGAGTGGCAAGGAGAAATTGAAACTTTGGTTCTTAACATTAGGGCTTTGGCTTCTTCGTTCTCTTCGTTTTCCTTCTTGCATGTGCCAAGGGAAGCGAATGGAGTGGCTCACCTGGTGGCTAAGAGGTCTTTGGAGTGG

Protein sequence

WSMPPKGVLKCNTNATCDSRRMKSGIGVVFRDDQNFLQAAMLATFPEISPLSAKVLATREAVRLAARLVPRVLFESDSLMVIQLIRGDLEWQGEIETLVLNIRALASSFSSFSFLHVPREANGVAHLVAKRSLEW

Homology

[Close]

BLAST of MC03g0153 vs. NCBI nr
Match: XP_022158489.1 (uncharacterized protein LOC111024968 [Momordica charantia])

HSP 1 Score: 80.5 bits (197), Expect = 2.16e-15
Identity = 53/134 (39.55%), Postives = 75/134 (55.97%), Query Frame = 0


Query: 1   WSMPPKGVLKCNTNATCDSRRMKSGIGVVFRDDQNFLQAAMLATFPEISPLSAKVLATRE 60
           W  P  GV+K NT+A        SG+GV+ R+    +  AM+      +PL AK+LA RE
Sbjct: 67  WRPPLGGVVKMNTDAAVSEEG--SGVGVLLREGNAEIVGAMVDFHMVKTPLLAKILAIRE 126

Query: 61  AVRLAARL-VPRVLFESDSLMVIQLIRGDLEWQGEIETLVLNIRALASSFSSFSFLHVPR 120
            + LA RL V RV+ E+DSL  + LI     W+GE  + V +IRA A  F    F HV R
Sbjct: 127 GLSLATRLGVHRVVVETDSLEALNLIGDKSPWKGEAVSWVEDIRAFARDFQEICFQHVFR 186

Query: 121 EANGVAHLVAKRSL 133
           E+N VA+ + + ++
Sbjct: 187 ESNAVAYHLGRETI 198

[Close]

BLAST of MC03g0153 vs. NCBI nr
Match: XP_030970368.1 (uncharacterized protein LOC115990707 [Quercus lobata])

HSP 1 Score: 74.7 bits (182), Expect = 9.82e-14
Identity = 52/132 (39.39%), Postives = 72/132 (54.55%), Query Frame = 0


Query: 1   WSMPPKGVLKCNTNATCDSRRMKSGIGVVFRDDQNFLQAAMLATFP-EISPLSAKVLATR 60
           W+ PP G +K NT+A       K  IGVV RD+   +  A     P   S   ++ LA +
Sbjct: 27  WNAPPTGFIKINTDAVAFEDGRKLCIGVVIRDNMGEVLVASNKVLPASFSTEVSEALALQ 86

Query: 61  EAVRLAARL-VPRVLFESDSLMVIQLIRGDLEWQGEIETLVLNIRALASSFSSFSFLHVP 120
           + V LAA L V   +FESD+L +I  I  ++   GE+  ++ NIR L + FS  SF H+ 
Sbjct: 87  DGVLLAAELEVSHAIFESDALSIILAINDEIHG-GELGHIIRNIRELTAVFSWCSFKHLK 146

Query: 121 REANGVAHLVAK 130
           RE N VAH +AK
Sbjct: 147 REGNRVAHELAK 157

[Close]

BLAST of MC03g0153 vs. NCBI nr
Match: XP_042983235.1 (uncharacterized protein LOC122312641 [Carya illinoinensis])

HSP 1 Score: 75.9 bits (185), Expect = 1.09e-13
Identity = 45/135 (33.33%), Postives = 72/135 (53.33%), Query Frame = 0


Query: 1   WSMPPKGVLKCNTNATCDSRRMKSGIGVVFRDDQNFLQAAMLATFPEI-SPLSAKVLATR 60
           WS PP    K N +A  D    K GIGV+ RD +  + A++ ++      PL  +  A  
Sbjct: 70  WSAPPPAFFKANWDAAIDKGSSKVGIGVIVRDSEGLVTASLCSSITLTPDPLLGEAAAAL 129

Query: 61  EAVRLAARL-VPRVLFESDSLMVIQLIRGDLEWQGEIETLVLNIRALASSFSSFSFLHVP 120
            A  L A L +  ++ E D+L V+Q ++G  E    +  ++ ++++L S F  +S  HVP
Sbjct: 130 RATTLCAELGLTHIVLEGDALNVVQAVQGVEENWSAVGMVIRDVKSLLSRFQEWSIHHVP 189

Query: 121 REANGVAHLVAKRSL 133
           RE N +AH +AK +L
Sbjct: 190 RELNVIAHTLAKYAL 204

[Close]

BLAST of MC03g0153 vs. NCBI nr
Match: XP_012487873.1 (PREDICTED: uncharacterized protein LOC105801085 [Gossypium raimondii])

HSP 1 Score: 75.1 bits (183), Expect = 1.63e-13
Identity = 42/135 (31.11%), Postives = 76/135 (56.30%), Query Frame = 0


Query: 1   WSMPPKGVLKCNTNATCDSRRMKSGIGVVFRDDQNFLQAAMLATFPEI-SPLSAKVLATR 60
           W  PP   +K N +A  D +  +S  G+V RD++  +  +    +  + SP +A+ LA+R
Sbjct: 44  WKKPPDQFVKINFDAAYDGKSKQSAAGIVARDNEGKVLLSCSMIYKHVASPFAAEALASR 103

Query: 61  EAVRLAARLVPR-VLFESDSLMVIQLIRGDLEWQGEIETLVLNIRALASSFSSFSFLHVP 120
           +A++    +  R ++ E DSL +I+  R ++  + ++   +++I  L SSF    F HVP
Sbjct: 104 KAIKTGKEMKWRKIIIEGDSLSIIKKCRSNIPDKSKMCAYIIDIHKLQSSFQECRFEHVP 163

Query: 121 REANGVAHLVAKRSL 133
           R  N +AH++AK +L
Sbjct: 164 RAVNSLAHMIAKATL 178

[Close]

BLAST of MC03g0153 vs. NCBI nr
Match: XP_030938250.1 (uncharacterized protein LOC115963402 [Quercus lobata])

HSP 1 Score: 75.5 bits (184), Expect = 3.40e-13
Identity = 53/134 (39.55%), Postives = 72/134 (53.73%), Query Frame = 0


Query: 1   WSMPPKGVLKCNTNATCDSRRMKSGIGVVFRDDQNFLQAAMLATFP-EISPLSAKVLATR 60
           W  PP G++K N +    S    +G+GV+ RD    + AA+  + P   S  + +V+A R
Sbjct: 121 WKPPPHGLVKINFDGAIFSTYSSAGLGVIIRDHAGLVLAALSQSIPLPTSVETVEVMAAR 180

Query: 61  EAVRLAARL-VPRVLFESDSLMVIQLIRGDLEWQGEIETLVLNIRALASSFSSFSFLHVP 120
            A+ LA  L   RV+ E DS  VIQ IR           L+ +I AL+ SFS  SFLHV 
Sbjct: 181 RALLLAKELGFERVVVEGDSEGVIQAIREKTLLSSGWGHLLKDIHALSLSFSCISFLHVK 240

Query: 121 REANGVAHLVAKRS 132
           R  N VAH +A+RS
Sbjct: 241 RSGNRVAHSLARRS 254

[Close]

BLAST of MC03g0153 vs. ExPASy TrEMBL
Match: A0A6J1DZK3 (uncharacterized protein LOC111024968 OS=Momordica charantia OX=3673 GN=LOC111024968 PE=4 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 1.05e-15
Identity = 53/134 (39.55%), Postives = 75/134 (55.97%), Query Frame = 0


Query: 1   WSMPPKGVLKCNTNATCDSRRMKSGIGVVFRDDQNFLQAAMLATFPEISPLSAKVLATRE 60
           W  P  GV+K NT+A        SG+GV+ R+    +  AM+      +PL AK+LA RE
Sbjct: 67  WRPPLGGVVKMNTDAAVSEEG--SGVGVLLREGNAEIVGAMVDFHMVKTPLLAKILAIRE 126

Query: 61  AVRLAARL-VPRVLFESDSLMVIQLIRGDLEWQGEIETLVLNIRALASSFSSFSFLHVPR 120
            + LA RL V RV+ E+DSL  + LI     W+GE  + V +IRA A  F    F HV R
Sbjct: 127 GLSLATRLGVHRVVVETDSLEALNLIGDKSPWKGEAVSWVEDIRAFARDFQEICFQHVFR 186

Query: 121 EANGVAHLVAKRSL 133
           E+N VA+ + + ++
Sbjct: 187 ESNAVAYHLGRETI 198

[Close]

BLAST of MC03g0153 vs. ExPASy TrEMBL
Match: A0A7J8YQW2 (Uncharacterized protein OS=Gossypium aridum OX=34290 GN=Goari_022918 PE=4 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 1.97e-13
Identity = 42/135 (31.11%), Postives = 78/135 (57.78%), Query Frame = 0


Query: 1   WSMPPKGVLKCNTNATCDSRRMKSGIGVVFRDDQNFLQAAMLATFPEI-SPLSAKVLATR 60
           W  PP  ++K N +A  D +  +S  G+V R++++ +  +    +  + SP +A+ LA+R
Sbjct: 254 WKKPPDQIVKINFDAAYDEKSKESAAGIVARNNESKVLLSCSMIYKHVASPFAAETLASR 313

Query: 61  EAVRLAARLVPR-VLFESDSLMVIQLIRGDLEWQGEIETLVLNIRALASSFSSFSFLHVP 120
           +A++    +  R ++ E DSL +I+  R ++  + ++ T + +I  L SSF    F HVP
Sbjct: 314 KAIKTGKEMKWRKIIIEGDSLSIIKKCRSNIPVKSKVCTYISDIHKLQSSFQECRFEHVP 373

Query: 121 REANGVAHLVAKRSL 133
           R  N +AH++AK +L
Sbjct: 374 RAINSLAHMIAKATL 388

[Close]

BLAST of MC03g0153 vs. ExPASy TrEMBL
Match: A0A2Z6PPU2 (Reverse transcriptase domain-containing protein OS=Trifolium subterraneum OX=3900 GN=TSUD_238260 PE=4 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 2.15e-13
Identity = 48/135 (35.56%), Postives = 71/135 (52.59%), Query Frame = 0


Query: 1   WSMPPKGVLKCNTNATCDSRRMKSGIGVVFRDDQNFLQAAMLATFP-EISPLSAKVLATR 60
           W  PP   LKCN +        K GIG+ FRD+      A   TFP E++    +  A +
Sbjct: 198 WEKPPANWLKCNVDGAIFMTERKFGIGICFRDNSGSFVQAHTMTFPFEVTAAECEATAMK 257

Query: 61  EAVRLA-ARLVPRVLFESDSLMVIQLIRGDLEWQGEIETLVLNIRALASSFSSFSFLHVP 120
            A+ LA +    RVLFESD   V+  +R D  +  E+ TL+    +L  S ++++  +V 
Sbjct: 258 HALALALSNGFERVLFESDCQQVVNALRNDYLYANELGTLLSTCSSLLISNANYNVAYVR 317

Query: 121 REANGVAHLVAKRSL 133
           R+AN VAH +A+ SL
Sbjct: 318 RQANRVAHNLARASL 332

[Close]

BLAST of MC03g0153 vs. ExPASy TrEMBL
Match: A0A2Z6NX36 (Reverse transcriptase domain-containing protein OS=Trifolium subterraneum OX=3900 GN=TSUD_277000 PE=4 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 4.52e-13
Identity = 48/135 (35.56%), Postives = 71/135 (52.59%), Query Frame = 0


Query: 1   WSMPPKGVLKCNTNATCDSRRMKSGIGVVFRDDQNFLQAAMLATFP-EISPLSAKVLATR 60
           W  PP   LKCN +        K GIG+ FRD       A   TFP E++ +  +  A +
Sbjct: 799 WEKPPVNWLKCNVDGAIFMTEGKFGIGICFRDSSGSFVQARTMTFPFEVTDVECEATAMK 858

Query: 61  EAVRLA-ARLVPRVLFESDSLMVIQLIRGDLEWQGEIETLVLNIRALASSFSSFSFLHVP 120
            A+ LA +    RVLFESD   V+  +R D  +  E+ TL+    +L  S ++++  +V 
Sbjct: 859 HALALALSNGFERVLFESDCQQVVNALRNDYLYANELGTLLSTCSSLLISNANYNVAYVR 918

Query: 121 REANGVAHLVAKRSL 133
           R+AN VAH +A+ SL
Sbjct: 919 RQANRVAHNLARASL 933

[Close]

BLAST of MC03g0153 vs. ExPASy TrEMBL
Match: A0A251NT15 (RNase H domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_6G141500 PE=4 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 1.83e-12
Identity = 50/135 (37.04%), Postives = 70/135 (51.85%), Query Frame = 0


Query: 1   WSMPPKGVLKCNTNATCDSRRMKSGIGVVFRD-DQNFLQAAMLATFPEISPLSAKVLATR 60
           W+ PP G LK N +   D    +  +GVV RD D  F+ A   +    +S   A++LA R
Sbjct: 119 WAAPPSGRLKFNFDGAFDPTSGRGAVGVVARDADGGFVAAVAKSVGEVLSAEHAEILAAR 178

Query: 61  EAVRLAARL-VPRVLFESDSLMVIQLIRGDLEWQGEIETLVLNIRALASSFSSFSFLHVP 120
           E V LA  L     +FE DS +V+  I+   +    I T+V +++ L   F S  F   P
Sbjct: 179 EGVALALSLGTASPIFEGDSAVVVSAIKRAGQDYSNIGTIVEDVKHLQQQFPSSLFQFTP 238

Query: 121 REANGVAHLVAKRSL 133
           REANGVAH +A+  L
Sbjct: 239 REANGVAHRLARFGL 253

[Close]

BLAST of MC03g0153 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 72.0 bits (175), Expect = 4.1e-13
Identity = 45/137 (32.85%), Postives = 77/137 (56.20%), Query Frame = 0


Query: 1   WSMPPKGVLKCNTNATCDSRRMKSGIGVVFRDDQNFLQAAMLATFPEI-SPLSAKVLATR 60
           W  PP   +KCNT+AT +    + GIG V R+++  ++       P++ S L A++ A R
Sbjct: 420 WRPPPHQWVKCNTDATWNRDNERCGIGWVLRNEKGEVKWMGARALPKLKSVLEAELEAMR 479

Query: 61  EAVRLAARL-VPRVLFESDSLMVIQLIRGDLEWQGEIETLVLNIRALASSFSSFSFLHVP 120
            AV   +R     V+FESDS ++I+++  D  W   ++  + +++ L S F+   F+ +P
Sbjct: 480 WAVLSLSRFQYNYVIFESDSQVLIEILNNDEIWP-SLKPTIQDLQRLLSQFTEVKFVFIP 539

Query: 121 REANGVAHLVAKRSLEW 136
           RE N +A  VA+ SL +
Sbjct: 540 REGNTLAERVARESLSF 555

[Close]

BLAST of MC03g0153 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 59.3 bits (142), Expect = 2.7e-09
Identity = 40/137 (29.20%), Postives = 66/137 (48.18%), Query Frame = 0


Query: 1   WSMPPKGVLKCNTNATCDSRRMKSGIGVVFRDDQNFLQAAMLATFPEI-SPLSAKVLATR 60
           W  PP   +KCNT+AT      + GIG + R++   +        P   + L A++ A R
Sbjct: 137 WKAPPYQWVKCNTDATWQLENPRCGIGWILRNESGGVLWMGARALPRTKNVLEAELEALR 196

Query: 61  EAVRLAARL-VPRVLFESDSLMVIQLIRGDLEWQGEIETLVLNIRALASSFSSFSFLHVP 120
            AV   +R    R++FESD+  ++ L+  D  W   ++  + +I+ L   F    F   P
Sbjct: 197 WAVLTMSRFNYKRIIFESDAQALVNLLNSDDFWP-TLQPALEDIQQLLHHFEEVKFEFTP 256

Query: 121 REANGVAHLVAKRSLEW 136
           R  N VA  +A+ S+ +
Sbjct: 257 RGGNKVADRIARESISF 272

[Close]

BLAST of MC03g0153 vs. TAIR 10
Match: AT5G65005.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 47.0 bits (110), Expect = 1.4e-05
Identity = 35/139 (25.18%), Postives = 64/139 (46.04%), Query Frame = 0


Query: 1   WSMPPKGVLKCNTNATCDSRRMKSGIGVVFRDDQNFLQAAMLATF-----PEISPLSAKV 60
           WS P +  LKCN +A+   R   SG+G + R+ Q  +    +  F      E +  S  +
Sbjct: 105 WSPPGRDKLKCNYDASHHERNTVSGLGWILRNSQGTVIECGMGKFQGRMTTEEAECSTLI 164

Query: 61  LATREAVRLAARLVPRVLFESDSLMVIQLIRGDLEWQGEIETLVLNIRALASSFSSFSFL 120
            A + +     +   +V+FE D+  + ++I         ++  +  I++   SF S  F 
Sbjct: 165 WAIQASYGFGHK---KVIFEGDNQTITRMINTKSS-NPRLQHFLDTIQSWIPSFESIEFS 224

Query: 121 HVPREANGVAHLVAKRSLE 135
              RE NG A  +AK++++
Sbjct: 225 FKHREQNGCADFLAKQAIK 239

The following BLAST results are available for this feature:

BLAST of MC03g0153 vs.


Match Name	E-value	Identity	Description

BLAST of MC03g0153 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158489.1	2.16e-15	39.55	uncharacterized protein LOC111024968 [Momordica charantia]	[more]
XP_030970368.1	9.82e-14	39.39	uncharacterized protein LOC115990707 [Quercus lobata]	[more]
XP_042983235.1	1.09e-13	33.33	uncharacterized protein LOC122312641 [Carya illinoinensis]	[more]
XP_012487873.1	1.63e-13	31.11	PREDICTED: uncharacterized protein LOC105801085 [Gossypium raimondii]	[more]
XP_030938250.1	3.40e-13	39.55	uncharacterized protein LOC115963402 [Quercus lobata]	[more]

BLAST of MC03g0153 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DZK3	1.05e-15	39.55	uncharacterized protein LOC111024968 OS=Momordica charantia OX=3673 GN=LOC111024...	[more]
A0A7J8YQW2	1.97e-13	31.11	Uncharacterized protein OS=Gossypium aridum OX=34290 GN=Goari_022918 PE=4 SV=1	[more]
A0A2Z6PPU2	2.15e-13	35.56	Reverse transcriptase domain-containing protein OS=Trifolium subterraneum OX=390...	[more]
A0A2Z6NX36	4.52e-13	35.56	Reverse transcriptase domain-containing protein OS=Trifolium subterraneum OX=390...	[more]
A0A251NT15	1.83e-12	37.04	RNase H domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_6G141500 PE...	[more]

BLAST of MC03g0153 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G29090.1	4.1e-13	32.85	Ribonuclease H-like superfamily protein	[more]
AT2G34320.1	2.7e-09	29.20	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT5G65005.1	1.4e-05	25.18	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 9..135 e-value: 1.1E-14 score: 56.6
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 12..131 e-value: 2.4E-21 score: 75.8
None	No IPR available	PANTHER	PTHR33033:SF67	SUBFAMILY NOT NAMED	coord: 1..131
None	No IPR available	PANTHER	PTHR33033	POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATED	coord: 1..131
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 11..130 e-value: 8.4589E-15 score: 63.8724
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 9..130

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC03g0153.1	MC03g0153.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity