MC01g1546 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC01g1546
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	NAD(P)-binding domain containing protein
Location	MC01: 19666724 .. 19670439 (+)
RNA-Seq Expression	MC01g1546
Synteny	MC01g1546

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

AAATTTGAAAACAGAAACAGAAAAGATGGTTCAGTCAGATTTATAAAAATTATATAGACTAAGGTTACGAGTTATCTAGTGCATTTATGAGTACGTATAGGAGCATTGTTTGTAGAACGAGACTCTACCAATTAGAAAATGCAAAATTTGAAATTAATAATAATTACCAATTGTATTTATCTGAAAGAAAAAAAAGAAAAAAAAATCAGACAGAAAAGAAGAAAGAAAACGGAGGAACTGGAAAGCGGGGCAGCGAGTAAAATAGAAATGGCGAATGGGTGGTTACATGGCGCCATTAGAGCCGCAGGAGAGAGATCCGTGGCTCTGCAATGGCCTTTCTTTTCCTTCCCACCGCCACCACTTCCTCCTTCTGCCAATTCAACTCTCAACGTCTCCATTTTTATCCTCTCTGCAAATCCTTTCTCGCCCTTTCCTACTTCTCCTCCAAGAAATCCCCCTCCCTCTCCATTCTCAACTCCAACAAGGTAATTCTTATTACTATTCTATCTCTGGTTGCCTCTGCGAAGTTGTTCTACCACCATTCACTGTTTAGTTACGAGAAAATGCTGGATTTCCATAACTTAACGAGTTGTTTAGGGCGTAATCTTACTCGCACTCTCAAGTGGGCTATTAGACTCGAGTTTCCTCTCCGCCGATCGCGCATATGATGTTGTGCAAAGGATCGGGAGGCCCCGAGTTTTAAATTTATGTTCATAATGTTCTTCGCGAGGTTTTTATGATCTCTAGTAATCTATTCTAAGCTCGATTTCCTGTTGGCAGATGGAAGGAAGTGAAATTACGGAAGAAGTTGCAGAGAAACAAAATGAGGACAATTTAAACGTGAAGAAAAAGATATTTGTGGCCGGTGCATCGGGAAGTACCGGAAAAAGGATTGTGGAGCAGTTGCTGGCAAGGGGTTTTGAAGTCAAGGCCGGGGTTCGAGACGTAAGCAAGGCGAAAACTACTCTTTCCCCTGGCAATCCTGATCTTCAAATTGTAAGTAAGTTTGTCCATTTTAAAAATTCCGATTCCATGGTAATTTGTTAGGTTGCGGAATATCTTGATTTCTTTCAGTTCTTAATATGTTAATAACATTTCAGGTGAAAGCTGATGTGACTGAAGGCTCTGCTAAGTTAGCAGAAGCCATAGGTTCCGATTCAGAAGCGGTAATCTGTGCTACAGGGTTTCGCCCAGGCTGGGATTTATTTGCGCCATGGAAGGTTTGCTTCTTACCATGAAACTATATTTGAAACATCAAACATAATTGTGAAAGTAGGAGCATCATCAAGTTAATGACCAGCTTGCTAATGCCAAGGGTTGAGTAGAGTAGTAAATTTACTCTTGGGTTGGACCTTGTCTCATGGATTTTAGACCCCTTTTGGATCCATTTAGATTAACCATCTGTGATAAAGATTGCTTGAGTTTAAAGTCTCTGTTCAAAGGTTTTGAAGTGCCAAAGAAAAAGTTGGAAAATATCTTGATGTGAATTGTGAAATTACCAGTAACTGCACACAGATTTCAAACAATTTCAGAATTCACATCAGTGAACCATAATATCCAAAGATTTCAAAACCCCAGGAACTTGTGTATGCTGAATATTCAACGTGGTGCTATCGTTACGCCATATTCTTCAAAGGTATAATCTAGAAACGATTACATTGCTGCAGGTTGACAACTATGGCACTGTAAACCTGGTTGAAGCATGTCGTCAGCTGGGCATAAACAGATTCATTCTCATCAGTTCAATTTTAGTCAATGGAGCTGCAATGGGGCAGATTCTCAACCCAGCTTACATCTTTCTGAATGTTTTTGGACTCACCTTGATGGCCAAGCTTCAGGCGGAGCAGCATATCAGGAAATCGGGTATTAACTACACGATTATTAGGCCTGGAGGTTTGAAAAATGATCCTCCCACTGGAAATTTAGTCATGACAGCAGAAGTAATCACATTCTCCCTTCTTGATCATTGCTTCTCTATGATATTTATGTGGCTAACTGAATCGTGGTTGTTTGTAGGATACTCTTTATGAAGGAAGCATATCAAGAGATTTAGTTGCAGAAGTAGCTGTCGAGGCATTGGTCCATTCTGAAGCCTCTTACAAGGTCGTTGAAATAGTTTCTCGTGCTGATGCGCCGAAGCGTACGTATGAGGATCTTTTTGGGTCCATCAAGCAGCGTTAGTTGTCTTTAGAGACCATCATATTTTCAGAATTATAGTGTTTGATATCTTGATTCAGCAGCAGTGCCTCATTTTCCAAGACCCCTTGGGGTCTTTAGCCTGTAATTCATAGCACTACGAATATACATGCATTTGCTAATGATGATGTGAAGCTTACATATTCTATCCTCTTTCTCAATAGGAAGATTAAGAATTACAAGAATTGAGGCATAAAAGACATCCTCCTCTTCCATGACATTAATTTTATAATACAAAGTGAAAATGAAGAAGTTTTGACAATGGAAAGGATTGATGGAAAGAACAAACAAAATCCATCATCTTTCCCCCCACAAGGATATATTGCTGTCAATGGAGGAAATCTTGTACTTGGTGGCAAGCTACACGCTATAATTCTATTCCTCAGTTAGGTTTGTCCAGAATTTGACGAAGCCTTCAACTTCTCTATTTGTTTCCGGAGATATGCCTGCCTCAGTTTCTCGCGCCGTGCTGTTGCCTGCTCTTGCCTGCGTAATTTCACCTCAGATGCCTTCTCTCTTGCTTGATTTACTTCTTCATGAACTTCCCTGAAGCAAACAAAGATCTCAACTTAAATCATTGAATGGAGGCCTCATTTCAGAACAAAGTTACAATGAAATCAGTGGCCAAGGGCTTGTTTGATACTTGATGTTCAAAAAAGTTTTCTACGTTAATCCTTAAAATTACAAAAAGTAACTATTTTCGTCCCTAAATTCTCATCACATTTGTTTTAGTTTCAAACTTTCATAACATAAATAGATTCTTAAACTTTCTAAACGAAACCATTTTGATACTTGCTGTATCATTTTTCCACCCAATATCTGTTTACCCCATACTCAGACTTCAACTTATGCAAATGTACTCACATAAACACATTAATACTCAAATCTACAAGATCAATTTATTAAAATCTAACCCATTAATACACACACTCAAATCTACAATATCAATTTGTTAAAATCAAAATACAGGTAAAGAAATAAAATGACTGTTTTCTTTTAAGTTCAAGGAGTGAAATAGATATTTTGAAAGTTCAGGACCAAAATAGATCGAACTTGAAAGTTCTGGAGGCAAAATACATGCTTTGAAAGTTTAGAAACCAAAACAAGATTTAAACCAATTCTATAAAAACATATTTGGATTACTGTCTTCTAAAAGTTGCTCTTTCTGGTTGTCTAATTAAATAGGGAAAGAGAAGTTCTACCTCACAAAGCGCTTGTGCTCATTGTTGTCCATTGTTCCAGACGTCCTCCCAAGTCCCGTTGTTTTTGGGTTAAACAAGAATGATGACTCAGTCTCATCTTCTGGAATGTCCATTTCTCCAGGTGCAGTAGCATCACGATCTTGCGATAGCTGTGATTCATCCTGCTCAAGATTCTTGCGGAAAAAATCGACACAATACTTCTCCTTATCTTCCTCGTCTGAAAGTTCCCCTTTCACTAGCTTGTCATATATCGCAGCCTTCTTCTCTAATGCCGCATAGCTTACCGATCCATCATTAATGGCCTTCAGCTCAAGCTTG

mRNA sequence

AAATTTGAAAACAGAAACAGAAAAGATGGTTCAGTCAGATTTATAAAAATTATATAGACTAAGGTTACGAGTTATCTAGTGCATTTATGAGTACGTATAGGAGCATTGTTTGTAGAACGAGACTCTACCAATTAGAAAATGCAAAATTTGAAATTAATAATAATTACCAATTGTATTTATCTGAAAGAAAAAAAAGAAAAAAAAATCAGACAGAAAAGAAGAAAGAAAACGGAGGAACTGGAAAGCGGGGCAGCGAGTAAAATAGAAATGGCGAATGGGTGGTTACATGGCGCCATTAGAGCCGCAGGAGAGAGATCCGTGGCTCTGCAATGGCCTTTCTTTTCCTTCCCACCGCCACCACTTCCTCCTTCTGCCAATTCAACTCTCAACGTCTCCATTTTTATCCTCTCTGCAAATCCTTTCTCGCCCTTTCCTACTTCTCCTCCAAGAAATCCCCCTCCCTCTCCATTCTCAACTCCAACAAGATGGAAGGAAGTGAAATTACGGAAGAAGTTGCAGAGAAACAAAATGAGGACAATTTAAACGTGAAGAAAAAGATATTTGTGGCCGGTGCATCGGGAAGTACCGGAAAAAGGATTGTGGAGCAGTTGCTGGCAAGGGGTTTTGAAGTCAAGGCCGGGGTTCGAGACGTAAGCAAGGCGAAAACTACTCTTTCCCCTGGCAATCCTGATCTTCAAATTGTGAAAGCTGATGTGACTGAAGGCTCTGCTAAGTTAGCAGAAGCCATAGGTTCCGATTCAGAAGCGGTAATCTGTGCTACAGGGTTTCGCCCAGGCTGGGATTTATTTGCGCCATGGAAGGTTGACAACTATGGCACTGTAAACCTGGTTGAAGCATGTCGTCAGCTGGGCATAAACAGATTCATTCTCATCAGTTCAATTTTAGTCAATGGAGCTGCAATGGGGCAGATTCTCAACCCAGCTTACATCTTTCTGAATGTTTTTGGACTCACCTTGATGGCCAAGCTTCAGGCGGAGCAGCATATCAGGAAATCGGGTATTAACTACACGATTATTAGGCCTGGAGGTTTGAAAAATGATCCTCCCACTGGAAATTTAGTCATGACAGCAGAAGATACTCTTTATGAAGGAAGCATATCAAGAGATTTAGTTGCAGAAGTAGCTGTCGAGGCATTGGTCCATTCTGAAGCCTCTTACAAGGTCGTTGAAATAGTTTCTCGTGCTGATGCGCCGAAGCGTACGTATGAGGATCTTTTTGGGTCCATCAAGCAGCGTTAGTTGTCTTTAGAGACCATCATATTTTCAGAATTATAGTGTTTGATATCTTGATTCAGCAGCAGTGCCTCATTTTCCAAGACCCCTTGGGGTCTTTAGCCTGTAATTCATAGCACTACGAATATACATGCATTTGCTAATGATGATGTGAAGCTTACATATTCTATCCTCTTTCTCAATAGGAAGATTAAGAATTACAAGAATTGAGGCATAAAAGACATCCTCCTCTTCCATGACATTAATTTTATAATACAAAGTGAAAATGAAGAAGTTTTGACAATGGAAAGGATTGATGGAAAGAACAAACAAAATCCATCATCTTTCCCCCCACAAGGATATATTGCTGTCAATGGAGGAAATCTTGTACTTGGTGGCAAGCTACACGCTATAATTCTATTCCTCAGTTAGGTTTGTCCAGAATTTGACGAAGCCTTCAACTTCTCTATTTGTTTCCGGAGATATGCCTGCCTCAGTTTCTCGCGCCGTGCTGTTGCCTGCTCTTGCCTGCGTAATTTCACCTCAGATGCCTTCTCTCTTGCTTGATTTACTTCTTCATGAACTTCCCTGAAGCAAACAAAGATCTCAACTTAAATCATTGAATGGAGGCCTCATTTCAGAACAAAGTTACAATGAAATCAGTGGCCAAGGGCTTGTTTGATACTTGATGTTCAAAAAAGTTTTCTACGTTAATCCTTAAAATTACAAAAAGTAACTATTTTCGTCCCTAAATTCTCATCACATTTGTTTTAGTTTCAAACTTTCATAACATAAATAGATTCTTAAACTTTCTAAACGAAACCATTTTGATACTTGCTGTATCATTTTTCCACCCAATATCTGTTTACCCCATACTCAGACTTCAACTTATGCAAATGTACTCACATAAACACATTAATACTCAAATCTACAAGATCAATTTATTAAAATCTAACCCATTAATACACACACTCAAATCTACAATATCAATTTGTTAAAATCAAAATACAGGTAAAGAAATAAAATGACTGTTTTCTTTTAAGTTCAAGGAGTGAAATAGATATTTTGAAAGTTCAGGACCAAAATAGATCGAACTTGAAAGTTCTGGAGGCAAAATACATGCTTTGAAAGTTTAGAAACCAAAACAAGATTTAAACCAATTCTATAAAAACATATTTGGATTACTGTCTTCTAAAAGTTGCTCTTTCTGGTTGTCTAATTAAATAGGGAAAGAGAAGTTCTACCTCACAAAGCGCTTGTGCTCATTGTTGTCCATTGTTCCAGACGTCCTCCCAAGTCCCGTTGTTTTTGGGTTAAACAAGAATGATGACTCAGTCTCATCTTCTGGAATGTCCATTTCTCCAGGTGCAGTAGCATCACGATCTTGCGATAGCTGTGATTCATCCTGCTCAAGATTCTTGCGGAAAAAATCGACACAATACTTCTCCTTATCTTCCTCGTCTGAAAGTTCCCCTTTCACTAGCTTGTCATATATCGCAGCCTTCTTCTCTAATGCCGCATAGCTTACCGATCCATCATTAATGGCCTTCAGCTCAAGCTTG

Coding sequence (CDS)

ATGGCCTTTCTTTTCCTTCCCACCGCCACCACTTCCTCCTTCTGCCAATTCAACTCTCAACGTCTCCATTTTTATCCTCTCTGCAAATCCTTTCTCGCCCTTTCCTACTTCTCCTCCAAGAAATCCCCCTCCCTCTCCATTCTCAACTCCAACAAGATGGAAGGAAGTGAAATTACGGAAGAAGTTGCAGAGAAACAAAATGAGGACAATTTAAACGTGAAGAAAAAGATATTTGTGGCCGGTGCATCGGGAAGTACCGGAAAAAGGATTGTGGAGCAGTTGCTGGCAAGGGGTTTTGAAGTCAAGGCCGGGGTTCGAGACGTAAGCAAGGCGAAAACTACTCTTTCCCCTGGCAATCCTGATCTTCAAATTGTGAAAGCTGATGTGACTGAAGGCTCTGCTAAGTTAGCAGAAGCCATAGGTTCCGATTCAGAAGCGGTAATCTGTGCTACAGGGTTTCGCCCAGGCTGGGATTTATTTGCGCCATGGAAGGTTGACAACTATGGCACTGTAAACCTGGTTGAAGCATGTCGTCAGCTGGGCATAAACAGATTCATTCTCATCAGTTCAATTTTAGTCAATGGAGCTGCAATGGGGCAGATTCTCAACCCAGCTTACATCTTTCTGAATGTTTTTGGACTCACCTTGATGGCCAAGCTTCAGGCGGAGCAGCATATCAGGAAATCGGGTATTAACTACACGATTATTAGGCCTGGAGGTTTGAAAAATGATCCTCCCACTGGAAATTTAGTCATGACAGCAGAAGATACTCTTTATGAAGGAAGCATATCAAGAGATTTAGTTGCAGAAGTAGCTGTCGAGGCATTGGTCCATTCTGAAGCCTCTTACAAGGTCGTTGAAATAGTTTCTCGTGCTGATGCGCCGAAGCGTACGTATGAGGATCTTTTTGGGTCCATCAAGCAGCGTTAG

Protein sequence

MAFLFLPTATTSSFCQFNSQRLHFYPLCKSFLALSYFSSKKSPSLSILNSNKMEGSEITEEVAEKQNEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFEVKAGVRDVSKAKTTLSPGNPDLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRPGWDLFAPWKVDNYGTVNLVEACRQLGINRFILISSILVNGAAMGQILNPAYIFLNVFGLTLMAKLQAEQHIRKSGINYTIIRPGGLKNDPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSEASYKVVEIVSRADAPKRTYEDLFGSIKQR

Homology

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BLAST of MC01g1546 vs. ExPASy Swiss-Prot
Match: Q8H124 (Uncharacterized protein At2g34460, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g34460 PE=1 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 3.1e-101
Identity = 192/276 (69.57%), Postives = 227/276 (82.25%), Query Frame = 0


Query: 32  LALSYFSSKKSPSLSILNSNKMEGSEITEEVAEKQNEDNLNVKKKIFVAGASGSTGKRIV 91
           L L + S+      S    NK   S  T    EK   +N    KK+FVAGA+G TGKRIV
Sbjct: 5   LLLRHSSAVFFSQSSFFTKNKSFRS-FTSIKMEKGEAENAVKTKKVFVAGATGQTGKRIV 64

Query: 92  EQLLARGFEVKAGVRDVSKAKTTLSPGNPDLQIVKADVTEGSAKLAEAIGSDSEAVICAT 151
           EQLL+RGF VKAGVRDV KAKT+    +P LQIV+ADVTEG  KLAE IG DS+AVICAT
Sbjct: 65  EQLLSRGFAVKAGVRDVEKAKTSFK-DDPSLQIVRADVTEGPDKLAEVIGDDSQAVICAT 124

Query: 152 GFRPGWDLFAPWKVDNYGTVNLVEACRQLGINRFILISSILVNGAAMGQILNPAYIFLNV 211
           GFRPG+D+F PWKVDN+GTVNLV+ACR+ G+ +F+L+SSILVNGAAMGQILNPAY+FLN+
Sbjct: 125 GFRPGFDIFTPWKVDNFGTVNLVDACRKQGVEKFVLVSSILVNGAAMGQILNPAYLFLNL 184

Query: 212 FGLTLMAKLQAEQHIRKSGINYTIIRPGGLKNDPPTGNLVMTAEDTLYEGSISRDLVAEV 271
           FGLTL+AKLQAE++I+KSGINYTI+RPGGLKNDPPTGN+VM  EDTLYEGSISRDLVAEV
Sbjct: 185 FGLTLVAKLQAEKYIKKSGINYTIVRPGGLKNDPPTGNVVMEPEDTLYEGSISRDLVAEV 244

Query: 272 AVEALVHSEASYKVVEIVSRADAPKRTYEDLFGSIK 308
           AVEAL+  E+S+KVVEIV+RA+APKR+Y+DLF S+K
Sbjct: 245 AVEALLQEESSFKVVEIVARAEAPKRSYKDLFASVK 278

[Close]

BLAST of MC01g1546 vs. ExPASy Swiss-Prot
Match: Q8SKU2 (Protein TIC 62, chloroplastic OS=Pisum sativum OX=3888 GN=TIC62 PE=1 SV=2)

HSP 1 Score: 122.5 bits (306), Expect = 8.5e-27
Identity = 102/310 (32.90%), Postives = 157/310 (50.65%), Query Frame = 0


Query: 19  SQRLHF--YPLCKSFLALSYFSSKKSPSLSILNSNKMEGSEITEEVAEKQNEDNLNVKKK 78
           S+  HF  YPL  +       SS  S S     ++    S   E + EK +  + N+   
Sbjct: 34  SKYSHFMRYPLTTTLTNNRIRSSSSSSSSIRAQASGSTKSSTAEGIPEKTDSKDDNL--- 93

Query: 79  IFVAGASGSTGKRIVEQLLARGFEVKAGVRDVSKA----------KTTLSPGNPDLQIVK 138
           +FVAGA+G  G R V +L+  GF+V+AGVR+  KA          K   + G  +  + K
Sbjct: 94  VFVAGATGKVGSRTVRELIKLGFKVRAGVRNAQKAGALVQSVKQLKLDGASGGGE-AVEK 153

Query: 139 ADVTEGSAKLAEAIGS---DSEAVICATGF--RPGWDLFAPWKVDNYGTVNLVEACRQLG 198
            ++ E   + A+ IGS   ++  VICA G   +  +D+  P ++D   T NLV+A     
Sbjct: 154 LEIVECDLEKADQIGSALGNASTVICAIGASEKEIFDITGPCRIDYRATKNLVDAATVAK 213

Query: 199 INRFILISSILVNGAAMGQILNPAYIFLNVFGLTLMAKLQAEQHIRKSGINYTIIRPGGL 258
           +N FIL++S+  N   +     PA I LN+F   L+ K +AE+ +  SGI YTI+RPGG+
Sbjct: 214 VNHFILVTSLGTNKFGL-----PAAI-LNLFWGVLIWKRKAEEALLASGIPYTIVRPGGM 273

Query: 259 KND----PPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSEASY-KVVEIVSRADAPK 307
           +        T N+ ++ EDTL+ G +S   VAE+      + + SY K+VE+++   AP 
Sbjct: 274 ERPTDAYKETHNVTLSTEDTLFGGQVSNLQVAELMAIMAKNPDLSYCKIVEVIAETTAPL 333

[Close]

BLAST of MC01g1546 vs. ExPASy Swiss-Prot
Match: Q8H0U5 (Protein TIC 62, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC62 PE=1 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 2.7e-25
Identity = 97/298 (32.55%), Postives = 149/298 (50.00%), Query Frame = 0


Query: 35  SYFSSKKSPSLSILNSNKMEGSEITEEVAEKQNEDNLNVKKK--IFVAGASGSTGKRIVE 94
           S     +S  LS+  S  +  S +  E     +  NLN K++  +FVAGA+G  G R V 
Sbjct: 44  SVSGKSRSFDLSLRASGPIRASSVVTEA----SPTNLNSKEEDLVFVAGATGKVGSRTVR 103

Query: 95  QLLARGFEVKAGVRDVSKAKTTLSP------GNPD--------LQIVKADVTEGSAKLAE 154
           +LL  GF V+AGVR   +A + +         N D        L+IV+ D+ E    +  
Sbjct: 104 ELLKLGFRVRAGVRSAQRAGSLVQSVKEMKLQNTDEGTQPVEKLEIVECDL-EKKDSIQP 163

Query: 155 AIGSDSEAVICATGF--RPGWDLFAPWKVDNYGTVNLVEACRQLGINRFILISSILVNGA 214
           A+G+ S  +IC  G   +   D+  P+++D   T NLV+A     +N FIL++S+  N  
Sbjct: 164 ALGNAS-VIICCIGASEKEISDITGPYRIDYLATKNLVDAATSAKVNNFILVTSLGTNKF 223

Query: 215 AMGQILNPAYIFLNVFGLTLMAKLQAEQHIRKSGINYTIIRPGGLKND----PPTGNLVM 274
                  PA I LN+F   L  K +AE+ + +SG+NY I+RPGG++        T NL +
Sbjct: 224 GF-----PAAI-LNLFWGVLCWKRKAEEALIESGLNYAIVRPGGMERPTDAYKETHNLTL 283

Query: 275 TAEDTLYEGSISRDLVAEVAVEALVHSEASY-KVVEIVSRADAPKRTYEDLFGSIKQR 310
             +DTL+ G +S   VAE+      + + S+ K+VE+V+   AP    E L   I  +
Sbjct: 284 ALDDTLFGGQVSNLQVAELLACMAKNPQLSFSKIVEVVAETTAPLTPIEKLLEKIPSK 329

[Close]

BLAST of MC01g1546 vs. ExPASy Swiss-Prot
Match: Q10A77 (Protein TIC 62, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=TIC62 PE=1 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 1.3e-22
Identity = 84/254 (33.07%), Postives = 131/254 (51.57%), Query Frame = 0


Query: 77  IFVAGASGSTGKRIVEQLLARGFEVKAGVRDVSKAKTTL-------------SPGNPDLQ 136
           +F+AGA+G  G R V + +  GF V+AGVR   +A + +             SP    L+
Sbjct: 75  VFIAGATGKVGSRAVREFIKLGFRVRAGVRSAQRASSLVQSVEQLKVDDDATSPAE-RLE 134

Query: 137 IVKADV-TEGSAKLAEAIGSDSEAVICATGF--RPGWDLFAPWKVDNYGTVNLVEACRQL 196
           IV+ D+  +  + +  AIG ++  V+C+ G   +   D+  P+++D   T NLV+A    
Sbjct: 135 IVECDLEKQAQSDIVSAIG-NAAIVVCSIGASEKDILDVTGPYRIDYMATNNLVQAATAA 194

Query: 197 GINRFILISSILVNGAAMGQILNPAYIFLNVFGLTLMAKLQAEQHIRKSGINYTIIRPGG 256
            +  FIL++S+  N     +I  PA++ LN+F   L  K +AE+ +  SG+ YTI+RPGG
Sbjct: 195 KVEHFILVTSLGTN-----RIGFPAFL-LNLFWGVLCWKRRAEEALIGSGLPYTIVRPGG 254

Query: 257 LKND----PPTGNLVMTAEDTLYEGSISRDLVAE-VAVEALVHSEASYKVVEIVSRADAP 310
           ++        T NLV+  EDT   G +S   VAE +A  A     A  KVVE ++   AP
Sbjct: 255 MERPTDAFKETHNLVVAVEDTYVGGLVSNLQVAELIACIASNRRTAYCKVVEAIAETTAP 314

[Close]

BLAST of MC01g1546 vs. ExPASy Swiss-Prot
Match: O07609 (Uncharacterized sugar epimerase YhfK OS=Bacillus subtilis (strain 168) OX=224308 GN=yhfK PE=2 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 3.7e-14
Identity = 65/222 (29.28%), Postives = 101/222 (45.50%), Query Frame = 0


Query: 76  KIFVAGASGSTGKRIVEQLLAR-GFEVKAGVRDVSKAKTTLSPGNPDLQIVKADVTEGSA 135
           K+F+ GA+G  G+R+V          ++A VR   + K +L     +  +      EGS 
Sbjct: 2   KVFLIGANGQIGQRLVSLFQDNPDHSIRAMVRK-EEQKASLEAAGAEAVLAN---LEGSP 61

Query: 136 KLAEAIGSDSEAVICATGFRPGWDLFAPWKVDNYGTVNLVEACRQLGINRFILISSILV- 195
           +   A     +A+I   G            VD  G    +EA    GI RFI++S++   
Sbjct: 62  EEIAAAAKGCDAIIFTAGSGGSTGYDKTLLVDLDGAAKAIEAAAIAGIKRFIMVSALQAH 121

Query: 196 NGAAMGQILNPAYIFLNVFGLTLMAKLQAEQHIRKSGINYTIIRPGGLKNDPPTGNLVMT 255
           N     + L P Y+          AK  A++ +  SG+ YTIIRPGGL+N+P TG   ++
Sbjct: 122 NRENWNEALKPYYV----------AKHYADKILEASGLTYTIIRPGGLRNEPGTG--TVS 181

Query: 256 AEDTLYEGSISRDLVAEVAVEALVHSEASYKVVEIVSRADAP 296
           A   L  G ISRD VA+  + +L       +  ++ +  D P
Sbjct: 182 AAKDLERGFISRDDVAKTVIASLDEKNTENRAFDL-TEGDTP 206

[Close]

BLAST of MC01g1546 vs. NCBI nr
Match: XP_022146656.1 (uncharacterized protein At2g34460, chloroplastic [Momordica charantia])

HSP 1 Score: 597 bits (1539), Expect = 7.22e-215
Identity = 309/309 (100.00%), Postives = 309/309 (100.00%), Query Frame = 0


Query: 1   MAFLFLPTATTSSFCQFNSQRLHFYPLCKSFLALSYFSSKKSPSLSILNSNKMEGSEITE 60
           MAFLFLPTATTSSFCQFNSQRLHFYPLCKSFLALSYFSSKKSPSLSILNSNKMEGSEITE
Sbjct: 1   MAFLFLPTATTSSFCQFNSQRLHFYPLCKSFLALSYFSSKKSPSLSILNSNKMEGSEITE 60

Query: 61  EVAEKQNEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFEVKAGVRDVSKAKTTLSPGNP 120
           EVAEKQNEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFEVKAGVRDVSKAKTTLSPGNP
Sbjct: 61  EVAEKQNEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFEVKAGVRDVSKAKTTLSPGNP 120

Query: 121 DLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRPGWDLFAPWKVDNYGTVNLVEACRQL 180
           DLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRPGWDLFAPWKVDNYGTVNLVEACRQL
Sbjct: 121 DLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRPGWDLFAPWKVDNYGTVNLVEACRQL 180

Query: 181 GINRFILISSILVNGAAMGQILNPAYIFLNVFGLTLMAKLQAEQHIRKSGINYTIIRPGG 240
           GINRFILISSILVNGAAMGQILNPAYIFLNVFGLTLMAKLQAEQHIRKSGINYTIIRPGG
Sbjct: 181 GINRFILISSILVNGAAMGQILNPAYIFLNVFGLTLMAKLQAEQHIRKSGINYTIIRPGG 240

Query: 241 LKNDPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSEASYKVVEIVSRADAPKRTYE 300
           LKNDPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSEASYKVVEIVSRADAPKRTYE
Sbjct: 241 LKNDPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSEASYKVVEIVSRADAPKRTYE 300

Query: 301 DLFGSIKQR 309
           DLFGSIKQR
Sbjct: 301 DLFGSIKQR 309

[Close]

BLAST of MC01g1546 vs. NCBI nr
Match: XP_022977850.1 (uncharacterized protein At2g34460, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 536 bits (1380), Expect = 1.14e-190
Identity = 283/309 (91.59%), Postives = 294/309 (95.15%), Query Frame = 0


Query: 1   MAFLFLPTATTSSFCQFNSQRLHFYPLCKSFLALSYFSSKKSPSLSILNSNKMEGSEITE 60
           MAFL LP A TSSF Q NSQRLHF PLCKSFLA+S  SSKK  S+SILNS KMEG+EITE
Sbjct: 1   MAFLSLPNAITSSFFQSNSQRLHFCPLCKSFLAVS--SSKKFASISILNSYKMEGNEITE 60

Query: 61  EVAEKQNEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFEVKAGVRDVSKAKTTLSPGNP 120
           EVAEKQ+EDNLNVKKKIFVAGASGSTGKRIVEQLLARGF VKAGVRDVSKAKTTLSPGNP
Sbjct: 61  EVAEKQSEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFAVKAGVRDVSKAKTTLSPGNP 120

Query: 121 DLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRPGWDLFAPWKVDNYGTVNLVEACRQL 180
           DLQIVKADVTEGSAKLAEAIGSDSEAVICATGFR GWDLFAPWKVDN+GTVNLV+ACR+L
Sbjct: 121 DLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRRGWDLFAPWKVDNFGTVNLVQACREL 180

Query: 181 GINRFILISSILVNGAAMGQILNPAYIFLNVFGLTLMAKLQAEQHIRKSGINYTIIRPGG 240
           GINRFILISSILVNGAAMGQILNPAYIFLNV GLTL+AKLQAEQHIRKSGINYTIIRPGG
Sbjct: 181 GINRFILISSILVNGAAMGQILNPAYIFLNVLGLTLIAKLQAEQHIRKSGINYTIIRPGG 240

Query: 241 LKNDPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSEASYKVVEIVSRADAPKRTYE 300
           LKN+PPTGNLVM AEDTLYEGSISRDLVAEVAVEALVHS+ASYKVVEIVSRADAPKR+YE
Sbjct: 241 LKNEPPTGNLVMAAEDTLYEGSISRDLVAEVAVEALVHSQASYKVVEIVSRADAPKRSYE 300

Query: 301 DLFGSIKQR 309
           DLFGSIKQR
Sbjct: 301 DLFGSIKQR 307

[Close]

BLAST of MC01g1546 vs. NCBI nr
Match: XP_022925949.1 (uncharacterized protein At2g34460, chloroplastic [Cucurbita moschata] >KAG6581421.1 putative protein, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034702.1 putative protein, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 536 bits (1380), Expect = 1.18e-190
Identity = 283/309 (91.59%), Postives = 294/309 (95.15%), Query Frame = 0


Query: 1   MAFLFLPTATTSSFCQFNSQRLHFYPLCKSFLALSYFSSKKSPSLSILNSNKMEGSEITE 60
           MAFL LP   TSSF Q NSQRLHF PLCKSFLA+S  SSKK  S+SILNS KMEG+EITE
Sbjct: 1   MAFLSLPNTITSSFFQSNSQRLHFCPLCKSFLAVSS-SSKKFASISILNSYKMEGNEITE 60

Query: 61  EVAEKQNEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFEVKAGVRDVSKAKTTLSPGNP 120
           EVAEKQ+EDNLNVKKKIFVAGASGSTGKRIVEQLLARGF VKAGVRDVSKAKTTLSPGNP
Sbjct: 61  EVAEKQSEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFAVKAGVRDVSKAKTTLSPGNP 120

Query: 121 DLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRPGWDLFAPWKVDNYGTVNLVEACRQL 180
           DLQIVKADVTEGSAKLAEAIGSDSEAVICATGFR GWDLFAPWKVDN+GTVNLV+ACR+L
Sbjct: 121 DLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRRGWDLFAPWKVDNFGTVNLVQACREL 180

Query: 181 GINRFILISSILVNGAAMGQILNPAYIFLNVFGLTLMAKLQAEQHIRKSGINYTIIRPGG 240
           GINRFILISSILVNGAAMGQILNPAYIFLNV GLTL+AKLQAEQHIRKSGINYTIIRPGG
Sbjct: 181 GINRFILISSILVNGAAMGQILNPAYIFLNVLGLTLIAKLQAEQHIRKSGINYTIIRPGG 240

Query: 241 LKNDPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSEASYKVVEIVSRADAPKRTYE 300
           LKN+PPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHS+ASYKVVEIVSRADAPKR+YE
Sbjct: 241 LKNEPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSQASYKVVEIVSRADAPKRSYE 300

Query: 301 DLFGSIKQR 309
           DLFGSIKQR
Sbjct: 301 DLFGSIKQR 308

[Close]

BLAST of MC01g1546 vs. NCBI nr
Match: XP_023544344.1 (uncharacterized protein At2g34460, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 533 bits (1373), Expect = 1.38e-189
Identity = 281/309 (90.94%), Postives = 294/309 (95.15%), Query Frame = 0


Query: 1   MAFLFLPTATTSSFCQFNSQRLHFYPLCKSFLALSYFSSKKSPSLSILNSNKMEGSEITE 60
           MAFL LP   TSSF Q NSQRLHF PLCKSFLA+S  SSKK  S+SILNS KMEG+EITE
Sbjct: 1   MAFLSLPNTITSSFFQSNSQRLHFCPLCKSFLAVSS-SSKKFASISILNSYKMEGNEITE 60

Query: 61  EVAEKQNEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFEVKAGVRDVSKAKTTLSPGNP 120
           EVAEKQ+EDNLNVKKKIFVAGASGSTGKRIVEQLLARGF VKAGVRDVSKAKTTLSPGNP
Sbjct: 61  EVAEKQSEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFAVKAGVRDVSKAKTTLSPGNP 120

Query: 121 DLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRPGWDLFAPWKVDNYGTVNLVEACRQL 180
           DLQIV+ADVTEGSAKLAEAIGSDSEAVICATGFR GWDLFAPWKVDN+GTVNLV+ACR+L
Sbjct: 121 DLQIVRADVTEGSAKLAEAIGSDSEAVICATGFRRGWDLFAPWKVDNFGTVNLVQACREL 180

Query: 181 GINRFILISSILVNGAAMGQILNPAYIFLNVFGLTLMAKLQAEQHIRKSGINYTIIRPGG 240
           GINRFILISSILVNGAAMGQILNPAYIFLNV GLTL+AKLQAE+HIRKSGINYTIIRPGG
Sbjct: 181 GINRFILISSILVNGAAMGQILNPAYIFLNVLGLTLIAKLQAEKHIRKSGINYTIIRPGG 240

Query: 241 LKNDPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSEASYKVVEIVSRADAPKRTYE 300
           LKN+PPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHS+ASYKVVEIVSRADAPKR+YE
Sbjct: 241 LKNEPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSQASYKVVEIVSRADAPKRSYE 300

Query: 301 DLFGSIKQR 309
           DLFGSIKQR
Sbjct: 301 DLFGSIKQR 308

[Close]

BLAST of MC01g1546 vs. NCBI nr
Match: XP_038881265.1 (uncharacterized protein At2g34460, chloroplastic [Benincasa hispida])

HSP 1 Score: 518 bits (1333), Expect = 1.48e-183
Identity = 274/309 (88.67%), Postives = 289/309 (93.53%), Query Frame = 0


Query: 1   MAFLFLPTATTSSFCQFNSQRLHFYPLCKSFLALSYFSSKKSPSLSILNSNKMEGSEITE 60
           MAFL LP A TSSF    SQRLHF PLCKSFL +S FSS + PS+SIL+S KMEG+EITE
Sbjct: 1   MAFLSLPNAITSSF----SQRLHFCPLCKSFLPVS-FSSNRFPSISILSSTKMEGNEITE 60

Query: 61  EVAEKQNEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFEVKAGVRDVSKAKTTLSPGNP 120
           EVAEKQ EDNLN+K+KIFVAGA+GSTGKRIVEQLLARGF VKAGVRDVSKAKTTLSPGN 
Sbjct: 61  EVAEKQIEDNLNMKRKIFVAGATGSTGKRIVEQLLARGFAVKAGVRDVSKAKTTLSPGNS 120

Query: 121 DLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRPGWDLFAPWKVDNYGTVNLVEACRQL 180
            LQIVKADVTEGSAKLAEAIGSDSEAVICATGFRPGWDLFAPWKVDN+GTVNLVEACR+L
Sbjct: 121 ALQIVKADVTEGSAKLAEAIGSDSEAVICATGFRPGWDLFAPWKVDNFGTVNLVEACREL 180

Query: 181 GINRFILISSILVNGAAMGQILNPAYIFLNVFGLTLMAKLQAEQHIRKSGINYTIIRPGG 240
           GINRFILISSILVNGAAMGQILNPAYIFLNV GLTL+AKLQAEQHIRKSGINYTIIRPGG
Sbjct: 181 GINRFILISSILVNGAAMGQILNPAYIFLNVLGLTLIAKLQAEQHIRKSGINYTIIRPGG 240

Query: 241 LKNDPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSEASYKVVEIVSRADAPKRTYE 300
           LKN+PPTGNLVMT EDTLYEGSISRDLV EVAVEALVHS+ASYKVVEIVSRADAPKR+Y+
Sbjct: 241 LKNEPPTGNLVMTPEDTLYEGSISRDLVGEVAVEALVHSQASYKVVEIVSRADAPKRSYD 300

Query: 301 DLFGSIKQR 309
           DLFGSIKQR
Sbjct: 301 DLFGSIKQR 304

[Close]

BLAST of MC01g1546 vs. ExPASy TrEMBL
Match: A0A6J1D008 (uncharacterized protein At2g34460, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111015805 PE=4 SV=1)

HSP 1 Score: 597 bits (1539), Expect = 3.50e-215
Identity = 309/309 (100.00%), Postives = 309/309 (100.00%), Query Frame = 0


Query: 1   MAFLFLPTATTSSFCQFNSQRLHFYPLCKSFLALSYFSSKKSPSLSILNSNKMEGSEITE 60
           MAFLFLPTATTSSFCQFNSQRLHFYPLCKSFLALSYFSSKKSPSLSILNSNKMEGSEITE
Sbjct: 1   MAFLFLPTATTSSFCQFNSQRLHFYPLCKSFLALSYFSSKKSPSLSILNSNKMEGSEITE 60

Query: 61  EVAEKQNEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFEVKAGVRDVSKAKTTLSPGNP 120
           EVAEKQNEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFEVKAGVRDVSKAKTTLSPGNP
Sbjct: 61  EVAEKQNEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFEVKAGVRDVSKAKTTLSPGNP 120

Query: 121 DLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRPGWDLFAPWKVDNYGTVNLVEACRQL 180
           DLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRPGWDLFAPWKVDNYGTVNLVEACRQL
Sbjct: 121 DLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRPGWDLFAPWKVDNYGTVNLVEACRQL 180

Query: 181 GINRFILISSILVNGAAMGQILNPAYIFLNVFGLTLMAKLQAEQHIRKSGINYTIIRPGG 240
           GINRFILISSILVNGAAMGQILNPAYIFLNVFGLTLMAKLQAEQHIRKSGINYTIIRPGG
Sbjct: 181 GINRFILISSILVNGAAMGQILNPAYIFLNVFGLTLMAKLQAEQHIRKSGINYTIIRPGG 240

Query: 241 LKNDPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSEASYKVVEIVSRADAPKRTYE 300
           LKNDPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSEASYKVVEIVSRADAPKRTYE
Sbjct: 241 LKNDPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSEASYKVVEIVSRADAPKRTYE 300

Query: 301 DLFGSIKQR 309
           DLFGSIKQR
Sbjct: 301 DLFGSIKQR 309

[Close]

BLAST of MC01g1546 vs. ExPASy TrEMBL
Match: A0A6J1INH1 (uncharacterized protein At2g34460, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111478024 PE=4 SV=1)

HSP 1 Score: 536 bits (1380), Expect = 5.53e-191
Identity = 283/309 (91.59%), Postives = 294/309 (95.15%), Query Frame = 0


Query: 1   MAFLFLPTATTSSFCQFNSQRLHFYPLCKSFLALSYFSSKKSPSLSILNSNKMEGSEITE 60
           MAFL LP A TSSF Q NSQRLHF PLCKSFLA+S  SSKK  S+SILNS KMEG+EITE
Sbjct: 1   MAFLSLPNAITSSFFQSNSQRLHFCPLCKSFLAVS--SSKKFASISILNSYKMEGNEITE 60

Query: 61  EVAEKQNEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFEVKAGVRDVSKAKTTLSPGNP 120
           EVAEKQ+EDNLNVKKKIFVAGASGSTGKRIVEQLLARGF VKAGVRDVSKAKTTLSPGNP
Sbjct: 61  EVAEKQSEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFAVKAGVRDVSKAKTTLSPGNP 120

Query: 121 DLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRPGWDLFAPWKVDNYGTVNLVEACRQL 180
           DLQIVKADVTEGSAKLAEAIGSDSEAVICATGFR GWDLFAPWKVDN+GTVNLV+ACR+L
Sbjct: 121 DLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRRGWDLFAPWKVDNFGTVNLVQACREL 180

Query: 181 GINRFILISSILVNGAAMGQILNPAYIFLNVFGLTLMAKLQAEQHIRKSGINYTIIRPGG 240
           GINRFILISSILVNGAAMGQILNPAYIFLNV GLTL+AKLQAEQHIRKSGINYTIIRPGG
Sbjct: 181 GINRFILISSILVNGAAMGQILNPAYIFLNVLGLTLIAKLQAEQHIRKSGINYTIIRPGG 240

Query: 241 LKNDPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSEASYKVVEIVSRADAPKRTYE 300
           LKN+PPTGNLVM AEDTLYEGSISRDLVAEVAVEALVHS+ASYKVVEIVSRADAPKR+YE
Sbjct: 241 LKNEPPTGNLVMAAEDTLYEGSISRDLVAEVAVEALVHSQASYKVVEIVSRADAPKRSYE 300

Query: 301 DLFGSIKQR 309
           DLFGSIKQR
Sbjct: 301 DLFGSIKQR 307

[Close]

BLAST of MC01g1546 vs. ExPASy TrEMBL
Match: A0A6J1EDM3 (uncharacterized protein At2g34460, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111433212 PE=4 SV=1)

HSP 1 Score: 536 bits (1380), Expect = 5.74e-191
Identity = 283/309 (91.59%), Postives = 294/309 (95.15%), Query Frame = 0


Query: 1   MAFLFLPTATTSSFCQFNSQRLHFYPLCKSFLALSYFSSKKSPSLSILNSNKMEGSEITE 60
           MAFL LP   TSSF Q NSQRLHF PLCKSFLA+S  SSKK  S+SILNS KMEG+EITE
Sbjct: 1   MAFLSLPNTITSSFFQSNSQRLHFCPLCKSFLAVSS-SSKKFASISILNSYKMEGNEITE 60

Query: 61  EVAEKQNEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFEVKAGVRDVSKAKTTLSPGNP 120
           EVAEKQ+EDNLNVKKKIFVAGASGSTGKRIVEQLLARGF VKAGVRDVSKAKTTLSPGNP
Sbjct: 61  EVAEKQSEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFAVKAGVRDVSKAKTTLSPGNP 120

Query: 121 DLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRPGWDLFAPWKVDNYGTVNLVEACRQL 180
           DLQIVKADVTEGSAKLAEAIGSDSEAVICATGFR GWDLFAPWKVDN+GTVNLV+ACR+L
Sbjct: 121 DLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRRGWDLFAPWKVDNFGTVNLVQACREL 180

Query: 181 GINRFILISSILVNGAAMGQILNPAYIFLNVFGLTLMAKLQAEQHIRKSGINYTIIRPGG 240
           GINRFILISSILVNGAAMGQILNPAYIFLNV GLTL+AKLQAEQHIRKSGINYTIIRPGG
Sbjct: 181 GINRFILISSILVNGAAMGQILNPAYIFLNVLGLTLIAKLQAEQHIRKSGINYTIIRPGG 240

Query: 241 LKNDPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSEASYKVVEIVSRADAPKRTYE 300
           LKN+PPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHS+ASYKVVEIVSRADAPKR+YE
Sbjct: 241 LKNEPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSQASYKVVEIVSRADAPKRSYE 300

Query: 301 DLFGSIKQR 309
           DLFGSIKQR
Sbjct: 301 DLFGSIKQR 308

[Close]

BLAST of MC01g1546 vs. ExPASy TrEMBL
Match: A0A5A7U9X6 (NAD(P)-binding Rossmann-fold superfamily protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G001550 PE=4 SV=1)

HSP 1 Score: 508 bits (1307), Expect = 7.57e-180
Identity = 266/308 (86.36%), Postives = 284/308 (92.21%), Query Frame = 0


Query: 1   MAFLFLPTATTSSFCQFNSQRLHFYPLCKSFLALSYFSSKKSPSLSILNSNKMEGSEITE 60
           MA LFLP A TSSF +FNSQRLHF PLC SFL+LS FSS +  S+SILNSNKMEG+EITE
Sbjct: 1   MASLFLPNAITSSFSKFNSQRLHFCPLCNSFLSLS-FSSNRFSSISILNSNKMEGNEITE 60

Query: 61  EVAEKQNEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFEVKAGVRDVSKAKTTLSPGNP 120
           EVA+ Q+E+N NVK+KIFVAGASGSTGK+IVEQLLARGF VKAGVRDVSKAKTTLSP NP
Sbjct: 61  EVAQTQSEENSNVKRKIFVAGASGSTGKKIVEQLLARGFAVKAGVRDVSKAKTTLSPANP 120

Query: 121 DLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRPGWDLFAPWKVDNYGTVNLVEACRQL 180
            LQIV+ADVTEGSAKLAEAIGSDSEAVICATGFR GWDLFAPWKVDN GTVNLVEACR L
Sbjct: 121 ALQIVRADVTEGSAKLAEAIGSDSEAVICATGFRRGWDLFAPWKVDNLGTVNLVEACRGL 180

Query: 181 GINRFILISSILVNGAAMGQILNPAYIFLNVFGLTLMAKLQAEQHIRKSGINYTIIRPGG 240
           GINRFILISSILVNGAAMGQILNPAYI LN  GL L+AKLQAE+HIR SGINYTIIRPGG
Sbjct: 181 GINRFILISSILVNGAAMGQILNPAYIILNALGLVLIAKLQAEKHIRNSGINYTIIRPGG 240

Query: 241 LKNDPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSEASYKVVEIVSRADAPKRTYE 300
           LKN+PPTGNLVMT EDTLYEGSISRDLVAEVAVEAL+HS+ASYKVVEIVSRADAPKR+YE
Sbjct: 241 LKNEPPTGNLVMTPEDTLYEGSISRDLVAEVAVEALLHSQASYKVVEIVSRADAPKRSYE 300

Query: 301 DLFGSIKQ 308
           DLFGS+KQ
Sbjct: 301 DLFGSVKQ 307

[Close]

BLAST of MC01g1546 vs. ExPASy TrEMBL
Match: A0A1S3AZV7 (uncharacterized protein At2g34460, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103484377 PE=4 SV=1)

HSP 1 Score: 508 bits (1307), Expect = 7.57e-180
Identity = 266/308 (86.36%), Postives = 284/308 (92.21%), Query Frame = 0


Query: 1   MAFLFLPTATTSSFCQFNSQRLHFYPLCKSFLALSYFSSKKSPSLSILNSNKMEGSEITE 60
           MA LFLP A TSSF +FNSQRLHF PLC SFL+LS FSS +  S+SILNSNKMEG+EITE
Sbjct: 1   MASLFLPNAITSSFSKFNSQRLHFCPLCNSFLSLS-FSSNRFSSISILNSNKMEGNEITE 60

Query: 61  EVAEKQNEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFEVKAGVRDVSKAKTTLSPGNP 120
           EVA+ Q+E+N NVK+KIFVAGASGSTGK+IVEQLLARGF VKAGVRDVSKAKTTLSP NP
Sbjct: 61  EVAQTQSEENSNVKRKIFVAGASGSTGKKIVEQLLARGFAVKAGVRDVSKAKTTLSPANP 120

Query: 121 DLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRPGWDLFAPWKVDNYGTVNLVEACRQL 180
            LQIV+ADVTEGSAKLAEAIGSDSEAVICATGFR GWDLFAPWKVDN GTVNLVEACR L
Sbjct: 121 ALQIVRADVTEGSAKLAEAIGSDSEAVICATGFRRGWDLFAPWKVDNLGTVNLVEACRGL 180

Query: 181 GINRFILISSILVNGAAMGQILNPAYIFLNVFGLTLMAKLQAEQHIRKSGINYTIIRPGG 240
           GINRFILISSILVNGAAMGQILNPAYI LN  GL L+AKLQAE+HIR SGINYTIIRPGG
Sbjct: 181 GINRFILISSILVNGAAMGQILNPAYIILNALGLVLIAKLQAEKHIRNSGINYTIIRPGG 240

Query: 241 LKNDPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSEASYKVVEIVSRADAPKRTYE 300
           LKN+PPTGNLVMT EDTLYEGSISRDLVAEVAVEAL+HS+ASYKVVEIVSRADAPKR+YE
Sbjct: 241 LKNEPPTGNLVMTPEDTLYEGSISRDLVAEVAVEALLHSQASYKVVEIVSRADAPKRSYE 300

Query: 301 DLFGSIKQ 308
           DLFGS+KQ
Sbjct: 301 DLFGSVKQ 307

[Close]

BLAST of MC01g1546 vs. TAIR 10
Match: AT2G34460.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 369.8 bits (948), Expect = 2.2e-102
Identity = 192/276 (69.57%), Postives = 227/276 (82.25%), Query Frame = 0


Query: 32  LALSYFSSKKSPSLSILNSNKMEGSEITEEVAEKQNEDNLNVKKKIFVAGASGSTGKRIV 91
           L L + S+      S    NK   S  T    EK   +N    KK+FVAGA+G TGKRIV
Sbjct: 5   LLLRHSSAVFFSQSSFFTKNKSFRS-FTSIKMEKGEAENAVKTKKVFVAGATGQTGKRIV 64

Query: 92  EQLLARGFEVKAGVRDVSKAKTTLSPGNPDLQIVKADVTEGSAKLAEAIGSDSEAVICAT 151
           EQLL+RGF VKAGVRDV KAKT+    +P LQIV+ADVTEG  KLAE IG DS+AVICAT
Sbjct: 65  EQLLSRGFAVKAGVRDVEKAKTSFK-DDPSLQIVRADVTEGPDKLAEVIGDDSQAVICAT 124

Query: 152 GFRPGWDLFAPWKVDNYGTVNLVEACRQLGINRFILISSILVNGAAMGQILNPAYIFLNV 211
           GFRPG+D+F PWKVDN+GTVNLV+ACR+ G+ +F+L+SSILVNGAAMGQILNPAY+FLN+
Sbjct: 125 GFRPGFDIFTPWKVDNFGTVNLVDACRKQGVEKFVLVSSILVNGAAMGQILNPAYLFLNL 184

Query: 212 FGLTLMAKLQAEQHIRKSGINYTIIRPGGLKNDPPTGNLVMTAEDTLYEGSISRDLVAEV 271
           FGLTL+AKLQAE++I+KSGINYTI+RPGGLKNDPPTGN+VM  EDTLYEGSISRDLVAEV
Sbjct: 185 FGLTLVAKLQAEKYIKKSGINYTIVRPGGLKNDPPTGNVVMEPEDTLYEGSISRDLVAEV 244

Query: 272 AVEALVHSEASYKVVEIVSRADAPKRTYEDLFGSIK 308
           AVEAL+  E+S+KVVEIV+RA+APKR+Y+DLF S+K
Sbjct: 245 AVEALLQEESSFKVVEIVARAEAPKRSYKDLFASVK 278

[Close]

BLAST of MC01g1546 vs. TAIR 10
Match: AT3G18890.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 117.5 bits (293), Expect = 1.9e-26
Identity = 97/298 (32.55%), Postives = 149/298 (50.00%), Query Frame = 0


Query: 35  SYFSSKKSPSLSILNSNKMEGSEITEEVAEKQNEDNLNVKKK--IFVAGASGSTGKRIVE 94
           S     +S  LS+  S  +  S +  E     +  NLN K++  +FVAGA+G  G R V 
Sbjct: 44  SVSGKSRSFDLSLRASGPIRASSVVTEA----SPTNLNSKEEDLVFVAGATGKVGSRTVR 103

Query: 95  QLLARGFEVKAGVRDVSKAKTTLSP------GNPD--------LQIVKADVTEGSAKLAE 154
           +LL  GF V+AGVR   +A + +         N D        L+IV+ D+ E    +  
Sbjct: 104 ELLKLGFRVRAGVRSAQRAGSLVQSVKEMKLQNTDEGTQPVEKLEIVECDL-EKKDSIQP 163

Query: 155 AIGSDSEAVICATGF--RPGWDLFAPWKVDNYGTVNLVEACRQLGINRFILISSILVNGA 214
           A+G+ S  +IC  G   +   D+  P+++D   T NLV+A     +N FIL++S+  N  
Sbjct: 164 ALGNAS-VIICCIGASEKEISDITGPYRIDYLATKNLVDAATSAKVNNFILVTSLGTNKF 223

Query: 215 AMGQILNPAYIFLNVFGLTLMAKLQAEQHIRKSGINYTIIRPGGLKND----PPTGNLVM 274
                  PA I LN+F   L  K +AE+ + +SG+NY I+RPGG++        T NL +
Sbjct: 224 GF-----PAAI-LNLFWGVLCWKRKAEEALIESGLNYAIVRPGGMERPTDAYKETHNLTL 283

Query: 275 TAEDTLYEGSISRDLVAEVAVEALVHSEASY-KVVEIVSRADAPKRTYEDLFGSIKQR 310
             +DTL+ G +S   VAE+      + + S+ K+VE+V+   AP    E L   I  +
Sbjct: 284 ALDDTLFGGQVSNLQVAELLACMAKNPQLSFSKIVEVVAETTAPLTPIEKLLEKIPSK 329

[Close]

BLAST of MC01g1546 vs. TAIR 10
Match: AT2G37660.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 75.1 bits (183), Expect = 1.1e-13
Identity = 82/295 (27.80%), Postives = 140/295 (47.46%), Query Frame = 0


Query: 37  FSSKKSPSLSILNSNKMEGS--EITEEVAEKQNEDNLNVKKKIFVAGASGSTGKRIVEQL 96
           F S  S S SI   +K  G    ++  V+     + L V     V GA G TG+ + ++L
Sbjct: 41  FRSLVSDSTSICGPSKFTGKNRRVSVTVSAAATTEPLTV----LVTGAGGRTGQIVYKKL 100

Query: 97  LARGFEVKAGVRDVSKAKTTLSPGNPDLQIVKADVTEGSAKLAEAIGSDSEAVICATG-- 156
             R  +  A  R + + K +    N + ++   D+ + +A +A A+      VI  +   
Sbjct: 101 KERSEQFVA--RGLVRTKESKEKINGEDEVFIGDIRD-TASIAPAVEGIDALVILTSAVP 160

Query: 157 -FRPGWDLFA-------------PWKVDNYGTVNLVEACRQLGINRFILISSILVNGAAM 216
             +PG+D                P +VD  G  N ++A +  G+ + +L+ S  + G  +
Sbjct: 161 QMKPGFDPSKGGRPEFFFDDGAYPEQVDWIGQKNQIDAAKAAGVKQIVLVGS--MGGTNI 220

Query: 217 GQILNPAYIFLNVFGLTLMAKLQAEQHIRKSGINYTIIRPGGLKN-DPPTGNLVMTAEDT 276
              LN +    N+    L+ K +AEQ++  SGI YTIIR GGL++ D     L++  +D 
Sbjct: 221 NHPLN-SIGNANI----LVWKRKAEQYLADSGIPYTIIRAGGLQDKDGGIRELLVGKDDE 280

Query: 277 LYE---GSISRDLVAEVAVEALVHSEASYKVVEIVSRAD---APKRTYEDLFGSI 307
           L E    +I+R  VAEV V+AL   EA +K +++ S+ +    P + ++ LF  +
Sbjct: 281 LLETETRTIARADVAEVCVQALQLEEAKFKALDLASKPEGTGTPTKDFKALFTQV 321

[Close]

BLAST of MC01g1546 vs. TAIR 10
Match: AT4G31530.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 70.9 bits (172), Expect = 2.1e-12
Identity = 87/293 (29.69%), Postives = 127/293 (43.34%), Query Frame = 0


Query: 26  PLCKSFLAL--SYFSSKKSPSLSILNSNKMEGSEITEEVAEKQNEDNLNVKKKIFVAGAS 85
           PL  S L L  S+F  +   SLS   S+       TEE  +  +  + +  K + V G +
Sbjct: 27  PLFTSPLPLPSSFFLVRNEASLS---SSITPVQAFTEEAVDTSDLAS-SSSKLVLVVGGT 86

Query: 86  GSTGKRIVEQLLARGFEVKAGVRDVSKAKTTLSPGNP-DLQIVKADVTEGSAKLAEAIGS 145
           G  G+ +V  LL R    +  +RD+ KA       +   LQ+VK D T  +  L  ++  
Sbjct: 87  GGVGQLVVASLLKRNIRSRLLLRDLDKATKLFGKQDEYSLQVVKGD-TRNAEDLDPSMFE 146

Query: 146 DSEAVICATGF----RPGW-DLFAPWKVDNYGTVNLVEACRQLGINRFILISSILVNGAA 205
               VIC TG        W +   P KVD  G  NL+ A     + R +L+SS+   G  
Sbjct: 147 GVTHVICTTGTTAFPSKRWNEENTPEKVDWEGVKNLISALPS-SVKRVVLVSSV---GVT 206

Query: 206 MGQILNPAYIFLNVFGLTLMAKLQAEQHIRKSGINYTIIRPGGLKNDPPTG--------- 265
               L   +  +N+FG+ L  K   E  +R SG+ +TIIRPG L + P T          
Sbjct: 207 KSNEL--PWSIMNLFGV-LKYKKMGEDFLRDSGLPFTIIRPGRLTDGPYTSYDLNTLLKA 266

Query: 266 -----NLVMTAEDTLYEGSISRDLVAEVAVEALVHSEASYKVVEIVS-RADAP 296
                  V+  +     G +SR +VAE  ++AL       K  EI S + D P
Sbjct: 267 TAGERRAVVIGQGDNLVGEVSRLVVAEACIQALDIEFTQGKAYEINSVKGDGP 307

[Close]

BLAST of MC01g1546 vs. TAIR 10
Match: AT4G31530.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 69.7 bits (169), Expect = 4.6e-12
Identity = 85/287 (29.62%), Postives = 124/287 (43.21%), Query Frame = 0


Query: 26  PLCKSFLAL--SYFSSKKSPSLSILNSNKMEGSEITEEVAEKQNEDNLNVKKKIFVAGAS 85
           PL  S L L  S+F  +   SLS   S+       TEE  +  +  + +  K + V G +
Sbjct: 27  PLFTSPLPLPSSFFLVRNEASLS---SSITPVQAFTEEAVDTSDLAS-SSSKLVLVVGGT 86

Query: 86  GSTGKRIVEQLLARGFEVKAGVRDVSKAKTTLSPGNP-DLQIVKADVTEGSAKLAEAIGS 145
           G  G+ +V  LL R    +  +RD+ KA       +   LQ+VK D T  +  L  ++  
Sbjct: 87  GGVGQLVVASLLKRNIRSRLLLRDLDKATKLFGKQDEYSLQVVKGD-TRNAEDLDPSMFE 146

Query: 146 DSEAVICATGF----RPGW-DLFAPWKVDNYGTVNLVEACRQLGINRFILISSILVNGAA 205
               VIC TG        W +   P KVD  G  NL+ A     + R +L+SS+   G  
Sbjct: 147 GVTHVICTTGTTAFPSKRWNEENTPEKVDWEGVKNLISALPS-SVKRVVLVSSV---GVT 206

Query: 206 MGQILNPAYIFLNVFGLTLMAKLQAEQHIRKSGINYTIIRPGGLKNDPPTG--------- 265
               L   +  +N+FG+ L  K   E  +R SG+ +TIIRPG L + P T          
Sbjct: 207 KSNEL--PWSIMNLFGV-LKYKKMGEDFLRDSGLPFTIIRPGRLTDGPYTSYDLNTLLKA 266

Query: 266 -----NLVMTAEDTLYEGSISRDLVAEVAVEALVHSEASYKVVEIVS 291
                  V+  +     G +SR +VAE  ++AL       K  EI S
Sbjct: 267 TAGERRAVVIGQGDNLVGEVSRLVVAEACIQALDIEFTQGKAYEINS 301

The following BLAST results are available for this feature:

BLAST of MC01g1546 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8H124	3.1e-101	69.57	Uncharacterized protein At2g34460, chloroplastic OS=Arabidopsis thaliana OX=3702...	[more]
Q8SKU2	8.5e-27	32.90	Protein TIC 62, chloroplastic OS=Pisum sativum OX=3888 GN=TIC62 PE=1 SV=2	[more]
Q8H0U5	2.7e-25	32.55	Protein TIC 62, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC62 PE=1 SV=1	[more]
Q10A77	1.3e-22	33.07	Protein TIC 62, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=TIC62 ...	[more]
O07609	3.7e-14	29.28	Uncharacterized sugar epimerase YhfK OS=Bacillus subtilis (strain 168) OX=224308...	[more]

BLAST of MC01g1546 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022146656.1	7.22e-215	100.00	uncharacterized protein At2g34460, chloroplastic [Momordica charantia]	[more]
XP_022977850.1	1.14e-190	91.59	uncharacterized protein At2g34460, chloroplastic-like [Cucurbita maxima]	[more]
XP_022925949.1	1.18e-190	91.59	uncharacterized protein At2g34460, chloroplastic [Cucurbita moschata] >KAG658142...	[more]
XP_023544344.1	1.38e-189	90.94	uncharacterized protein At2g34460, chloroplastic-like [Cucurbita pepo subsp. pep...	[more]
XP_038881265.1	1.48e-183	88.67	uncharacterized protein At2g34460, chloroplastic [Benincasa hispida]	[more]

BLAST of MC01g1546 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D008	3.50e-215	100.00	uncharacterized protein At2g34460, chloroplastic OS=Momordica charantia OX=3673 ...	[more]
A0A6J1INH1	5.53e-191	91.59	uncharacterized protein At2g34460, chloroplastic-like OS=Cucurbita maxima OX=366...	[more]
A0A6J1EDM3	5.74e-191	91.59	uncharacterized protein At2g34460, chloroplastic OS=Cucurbita moschata OX=3662 G...	[more]
A0A5A7U9X6	7.57e-180	86.36	NAD(P)-binding Rossmann-fold superfamily protein OS=Cucumis melo var. makuwa OX=...	[more]
A0A1S3AZV7	7.57e-180	86.36	uncharacterized protein At2g34460, chloroplastic OS=Cucumis melo OX=3656 GN=LOC1...	[more]

BLAST of MC01g1546 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G34460.1	2.2e-102	69.57	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G18890.1	1.9e-26	32.55	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G37660.1	1.1e-13	27.80	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G31530.1	2.1e-12	29.69	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G31530.2	4.6e-12	29.62	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.720		coord: 55..300 e-value: 5.9E-50 score: 171.9
None	No IPR available	PANTHER	PTHR15020:SF39	SUBFAMILY NOT NAMED	coord: 53..309
None	No IPR available	PANTHER	PTHR15020	FLAVIN REDUCTASE-RELATED	coord: 53..309
None	No IPR available	CDD	cd05243	SDR_a5	coord: 76..291 e-value: 5.1254E-58 score: 183.591
IPR016040	NAD(P)-binding domain	PFAM	PF13460	NAD_binding_10	coord: 81..278 e-value: 8.7E-45 score: 152.8
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 75..290

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC01g1546.1	MC01g1546.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007049	cell cycle
biological_process	GO:0032508	DNA duplex unwinding
biological_process	GO:0006270	DNA replication initiation
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0042555	MCM complex
cellular_component	GO:0000347	THO complex
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016887	ATP hydrolysis activity
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003678	DNA helicase activity