Lsi05G005990 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi05G005990
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionnuclear intron maturase 3, mitochondrial
Locationchr05: 7420865 .. 7423168 (+)
RNA-Seq ExpressionLsi05G005990
SyntenyLsi05G005990
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTCATCCAGTTCAGAAAAATAGCGACTCCATTGAAACCCAAGTTCTCAAATTCCCTCACCAAGCCTTACTTCCATCTGCCATTGAAATTGAAGCATTCGTCTCTGCTCAAGCTCCCGTCTCAACATTCTCCAGAAACCCTCACAAGGCCCCAACTCAAGGCTCTAGTTCTCAGCCATTTCTCCCAGGGCAAGTTCTTCGACCTTTTTCAAAATGTTGTCGCCTCACCCTCTGTTCTTCTCACTGCCTCCCAAAACCTCATCAGTTCGCCTCTCAGTAATGCCCCCGATTCCTTACCTGTTTTTGAATTGGTCTCCAATTGCTTTTCGGTCGAGGTCATGGCTCGGGAGCTCTCCGAAAATCGTTTTGATGTTGGAGCTTGCTGTGTTCGGATGGCACCATTGGAAGAGAAAGGTGAGTCTCTGGTCTTACCGAATTTGAAATTGAAGGTCTTAATCGAGGCAATTAGGATAGTGTTGGAAATTGTTTATGATGAACGATTTGTAACGTTCTCGTATGGTGGGCGTGTCGGTATGGGGCGACACACTGCGATTAGATACCTGAAAAACTCAGTACAAAACCCTAGTTGGTGGTTTACTGTTGCATTTCGTCACAAAAAGTTTGAATCTGTACATGTAAATACGTTGTGCTTATTGATGCAAGAGAAAATTAAGGATGATACTTTGATTTATATGTTAAGGAAACTGTTTGAATTGGAAGCAATTCAAATTGAATTGGGTGCTTTTTATTTAGGAAGGGGTTTCCCTCAGGAAAGTAGTTTGTGTTCTATCTTGTTTAATATATACTTCAATGGCTTTGATAAAGAAATTCAACAAATACGTCTCCAAAAAAATGAAGAAAATCCTAAGTTCAAGCTGGACGAGATTGTTTCTTTTCATAATCCGGTGAAAGTATATGCTGTTAGATATCTGGATGAGATATTAGTTATAACATCAGGGTCGAAGATGCTAACAATGGAGTTGAAAAGCCAGGTGCTAAAGTATTTAGAAGGGAATTTAGAATTGGAAGTGGATCGAATGAATACTGCGATTCATAGTGCTGTCTCAGAGAAAATTAGTTTCTTAGGAATGGAACTACAGGCGGTACCACCTTCAGTTCTGCATCCACCAATGTCGGAGAAGGCAGTCAGGGCAAGGAAGAAATACCTTAGACAAAAGGAAGTTAGAGCGATCGAATTGAGAAATGCCCGTGAGAGAAACAGGAAAAAATTGGGATTGAAGATATTGAGTCATGTGTTCAAGAAATTGAAGCAAACTAGTGGGTTTAAATCTGAATTCCAAATTGAGAAGGAAGTTAGAGATATCTTCAGAAACTGGGCTGATGAAGTGGTGCAAGATTTCTTTGAGTCCTCTGAAGATCATGCAGAGTGGCACCGTCTGCTGTCAGCAGGTGATTTCCTCTCTTTAAAACACATAAGAAATCAATTGCCAGAAAATCTTGTGAATGCTTATGATAGGTTTCAAGATCAAGTAAACAAGCACTTGAATCCTGTAAAGGTTAAAAAGGAAAAGGCTCGGGAGGATGAAGAGAAAAGAGTGGAGGAGGAAGAACTATATGCTAAAAGAACAGTTGAGGACTTAACAAGGCTATGCATCAAAGTCGATGCTCCTATAGAGCTTGTTAGGAAGGCAGTCAAGATGGTTGGGTTTACAAATAAAATGGGTCGTCCTCGGCCAATCAGCTCACTCCTTGCTCTTGAAGACGCTGATATTATCAAGTGGTATTCTGGTGTTGGAAGACGGTGGTTAGACTTCTTTTGCTGTTGTCATAACTATAAGACGGTCAAAACTGTTGTAACTTACCACTTAAGGTTTTCTTGTATTTTGACATTGGCAGAAAAGCATGAATCAACCAAACGGGAAGCCATGAAACATTACAGTAAGGATTTGAAAGTCTCTGATCTGAATGGCAATGAAGAAATGCACTTCCCAACAGAAAGAGAAGTTAAGATGTTGGGAGAAAGAAATCTTGTCGATCCATACCCTGTGGATGGGGCTTTATCTTTGCTTCTGATTCGGTTAGCCACTGATGAAGCTTCATATCCTTGTATTGCTCATTTTTGCAATAGAACAGACTCTATTTTATATCGTGTTCGATTACTGCAAAGGACTCTGAATGTCAATCCATCTAATGGAGTGGAATGGGTTAGAGGAATGGGAGTGATTCATGAAAGTTTAAATCAGAGATGCCTTCCTCTCTGTGCTGATCACATTAGTGATTTATACATGGGGAAAATCAACCTTCAAGACTTGGACTGCACCTTATCATTGGATATGGACTGA

mRNA sequence

ATGTTCATCCAGTTCAGAAAAATAGCGACTCCATTGAAACCCAAGTTCTCAAATTCCCTCACCAAGCCTTACTTCCATCTGCCATTGAAATTGAAGCATTCGTCTCTGCTCAAGCTCCCGTCTCAACATTCTCCAGAAACCCTCACAAGGCCCCAACTCAAGGCTCTAGTTCTCAGCCATTTCTCCCAGGGCAAGTTCTTCGACCTTTTTCAAAATGTTGTCGCCTCACCCTCTGTTCTTCTCACTGCCTCCCAAAACCTCATCAGTTCGCCTCTCAGTAATGCCCCCGATTCCTTACCTGTTTTTGAATTGGTCTCCAATTGCTTTTCGGTCGAGGTCATGGCTCGGGAGCTCTCCGAAAATCGTTTTGATGTTGGAGCTTGCTGTGTTCGGATGGCACCATTGGAAGAGAAAGGTGAGTCTCTGGTCTTACCGAATTTGAAATTGAAGGTCTTAATCGAGGCAATTAGGATAGTGTTGGAAATTGTTTATGATGAACGATTTGTAACGTTCTCGTATGGTGGGCGTGTCGGTATGGGGCGACACACTGCGATTAGATACCTGAAAAACTCAGTACAAAACCCTAGTTGGTGGTTTACTGTTGCATTTCGTCACAAAAAGTTTGAATCTGTACATGTAAATACGTTGTGCTTATTGATGCAAGAGAAAATTAAGGATGATACTTTGATTTATATGTTAAGGAAACTGTTTGAATTGGAAGCAATTCAAATTGAATTGGGTGCTTTTTATTTAGGAAGGGGTTTCCCTCAGGAAAGTAGTTTGTGTTCTATCTTGTTTAATATATACTTCAATGGCTTTGATAAAGAAATTCAACAAATACGTCTCCAAAAAAATGAAGAAAATCCTAAGTTCAAGCTGGACGAGATTGTTTCTTTTCATAATCCGGTGAAAGTATATGCTGTTAGATATCTGGATGAGATATTAGTTATAACATCAGGGTCGAAGATGCTAACAATGGAGTTGAAAAGCCAGGTGCTAAAGTATTTAGAAGGGAATTTAGAATTGGAAGTGGATCGAATGAATACTGCGATTCATAGTGCTGTCTCAGAGAAAATTAGTTTCTTAGGAATGGAACTACAGGCGGTACCACCTTCAGTTCTGCATCCACCAATGTCGGAGAAGGCAGTCAGGGCAAGGAAGAAATACCTTAGACAAAAGGAAGTTAGAGCGATCGAATTGAGAAATGCCCGTGAGAGAAACAGGAAAAAATTGGGATTGAAGATATTGAGTCATGTGTTCAAGAAATTGAAGCAAACTAGTGGGTTTAAATCTGAATTCCAAATTGAGAAGGAAGTTAGAGATATCTTCAGAAACTGGGCTGATGAAGTGGTGCAAGATTTCTTTGAGTCCTCTGAAGATCATGCAGAGTGGCACCGTCTGCTGTCAGCAGGTGATTTCCTCTCTTTAAAACACATAAGAAATCAATTGCCAGAAAATCTTGTGAATGCTTATGATAGGTTTCAAGATCAAGTAAACAAGCACTTGAATCCTGTAAAGGTTAAAAAGGAAAAGGCTCGGGAGGATGAAGAGAAAAGAGTGGAGGAGGAAGAACTATATGCTAAAAGAACAGTTGAGGACTTAACAAGGCTATGCATCAAAGTCGATGCTCCTATAGAGCTTGTTAGGAAGGCAGTCAAGATGGTTGGGTTTACAAATAAAATGGGTCGTCCTCGGCCAATCAGCTCACTCCTTGCTCTTGAAGACGCTGATATTATCAAGTGGTATTCTGGTGTTGGAAGACGGTGGTTAGACTTCTTTTGCTGTTGTCATAACTATAAGACGGTCAAAACTGTTGTAACTTACCACTTAAGGTTTTCTTGTATTTTGACATTGGCAGAAAAGCATGAATCAACCAAACGGGAAGCCATGAAACATTACAGTAAGGATTTGAAAGTCTCTGATCTGAATGGCAATGAAGAAATGCACTTCCCAACAGAAAGAGAAGTTAAGATGTTGGGAGAAAGAAATCTTGTCGATCCATACCCTGTGGATGGGGCTTTATCTTTGCTTCTGATTCGGTTAGCCACTGATGAAGCTTCATATCCTTGTATTGCTCATTTTTGCAATAGAACAGACTCTATTTTATATCGTGTTCGATTACTGCAAAGGACTCTGAATGTCAATCCATCTAATGGAGTGGAATGGGTTAGAGGAATGGGAGTGATTCATGAAAGTTTAAATCAGAGATGCCTTCCTCTCTGTGCTGATCACATTAGTGATTTATACATGGGGAAAATCAACCTTCAAGACTTGGACTGCACCTTATCATTGGATATGGACTGA

Coding sequence (CDS)

ATGTTCATCCAGTTCAGAAAAATAGCGACTCCATTGAAACCCAAGTTCTCAAATTCCCTCACCAAGCCTTACTTCCATCTGCCATTGAAATTGAAGCATTCGTCTCTGCTCAAGCTCCCGTCTCAACATTCTCCAGAAACCCTCACAAGGCCCCAACTCAAGGCTCTAGTTCTCAGCCATTTCTCCCAGGGCAAGTTCTTCGACCTTTTTCAAAATGTTGTCGCCTCACCCTCTGTTCTTCTCACTGCCTCCCAAAACCTCATCAGTTCGCCTCTCAGTAATGCCCCCGATTCCTTACCTGTTTTTGAATTGGTCTCCAATTGCTTTTCGGTCGAGGTCATGGCTCGGGAGCTCTCCGAAAATCGTTTTGATGTTGGAGCTTGCTGTGTTCGGATGGCACCATTGGAAGAGAAAGGTGAGTCTCTGGTCTTACCGAATTTGAAATTGAAGGTCTTAATCGAGGCAATTAGGATAGTGTTGGAAATTGTTTATGATGAACGATTTGTAACGTTCTCGTATGGTGGGCGTGTCGGTATGGGGCGACACACTGCGATTAGATACCTGAAAAACTCAGTACAAAACCCTAGTTGGTGGTTTACTGTTGCATTTCGTCACAAAAAGTTTGAATCTGTACATGTAAATACGTTGTGCTTATTGATGCAAGAGAAAATTAAGGATGATACTTTGATTTATATGTTAAGGAAACTGTTTGAATTGGAAGCAATTCAAATTGAATTGGGTGCTTTTTATTTAGGAAGGGGTTTCCCTCAGGAAAGTAGTTTGTGTTCTATCTTGTTTAATATATACTTCAATGGCTTTGATAAAGAAATTCAACAAATACGTCTCCAAAAAAATGAAGAAAATCCTAAGTTCAAGCTGGACGAGATTGTTTCTTTTCATAATCCGGTGAAAGTATATGCTGTTAGATATCTGGATGAGATATTAGTTATAACATCAGGGTCGAAGATGCTAACAATGGAGTTGAAAAGCCAGGTGCTAAAGTATTTAGAAGGGAATTTAGAATTGGAAGTGGATCGAATGAATACTGCGATTCATAGTGCTGTCTCAGAGAAAATTAGTTTCTTAGGAATGGAACTACAGGCGGTACCACCTTCAGTTCTGCATCCACCAATGTCGGAGAAGGCAGTCAGGGCAAGGAAGAAATACCTTAGACAAAAGGAAGTTAGAGCGATCGAATTGAGAAATGCCCGTGAGAGAAACAGGAAAAAATTGGGATTGAAGATATTGAGTCATGTGTTCAAGAAATTGAAGCAAACTAGTGGGTTTAAATCTGAATTCCAAATTGAGAAGGAAGTTAGAGATATCTTCAGAAACTGGGCTGATGAAGTGGTGCAAGATTTCTTTGAGTCCTCTGAAGATCATGCAGAGTGGCACCGTCTGCTGTCAGCAGGTGATTTCCTCTCTTTAAAACACATAAGAAATCAATTGCCAGAAAATCTTGTGAATGCTTATGATAGGTTTCAAGATCAAGTAAACAAGCACTTGAATCCTGTAAAGGTTAAAAAGGAAAAGGCTCGGGAGGATGAAGAGAAAAGAGTGGAGGAGGAAGAACTATATGCTAAAAGAACAGTTGAGGACTTAACAAGGCTATGCATCAAAGTCGATGCTCCTATAGAGCTTGTTAGGAAGGCAGTCAAGATGGTTGGGTTTACAAATAAAATGGGTCGTCCTCGGCCAATCAGCTCACTCCTTGCTCTTGAAGACGCTGATATTATCAAGTGGTATTCTGGTGTTGGAAGACGGTGGTTAGACTTCTTTTGCTGTTGTCATAACTATAAGACGGTCAAAACTGTTGTAACTTACCACTTAAGGTTTTCTTGTATTTTGACATTGGCAGAAAAGCATGAATCAACCAAACGGGAAGCCATGAAACATTACAGTAAGGATTTGAAAGTCTCTGATCTGAATGGCAATGAAGAAATGCACTTCCCAACAGAAAGAGAAGTTAAGATGTTGGGAGAAAGAAATCTTGTCGATCCATACCCTGTGGATGGGGCTTTATCTTTGCTTCTGATTCGGTTAGCCACTGATGAAGCTTCATATCCTTGTATTGCTCATTTTTGCAATAGAACAGACTCTATTTTATATCGTGTTCGATTACTGCAAAGGACTCTGAATGTCAATCCATCTAATGGAGTGGAATGGGTTAGAGGAATGGGAGTGATTCATGAAAGTTTAAATCAGAGATGCCTTCCTCTCTGTGCTGATCACATTAGTGATTTATACATGGGGAAAATCAACCTTCAAGACTTGGACTGCACCTTATCATTGGATATGGACTGA

Protein sequence

MFIQFRKIATPLKPKFSNSLTKPYFHLPLKLKHSSLLKLPSQHSPETLTRPQLKALVLSHFSQGKFFDLFQNVVASPSVLLTASQNLISSPLSNAPDSLPVFELVSNCFSVEVMARELSENRFDVGACCVRMAPLEEKGESLVLPNLKLKVLIEAIRIVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKNSVQNPSWWFTVAFRHKKFESVHVNTLCLLMQEKIKDDTLIYMLRKLFELEAIQIELGAFYLGRGFPQESSLCSILFNIYFNGFDKEIQQIRLQKNEENPKFKLDEIVSFHNPVKVYAVRYLDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRMNTAIHSAVSEKISFLGMELQAVPPSVLHPPMSEKAVRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVFKKLKQTSGFKSEFQIEKEVRDIFRNWADEVVQDFFESSEDHAEWHRLLSAGDFLSLKHIRNQLPENLVNAYDRFQDQVNKHLNPVKVKKEKAREDEEKRVEEEELYAKRTVEDLTRLCIKVDAPIELVRKAVKMVGFTNKMGRPRPISSLLALEDADIIKWYSGVGRRWLDFFCCCHNYKTVKTVVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVSDLNGNEEMHFPTEREVKMLGERNLVDPYPVDGALSLLLIRLATDEASYPCIAHFCNRTDSILYRVRLLQRTLNVNPSNGVEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD
Homology
[Close]
BLAST of Lsi05G005990 vs. ExPASy Swiss-Prot
Match: Q9LZA5 (Nuclear intron maturase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NMAT3 PE=3 SV=2)

HSP 1 Score: 807.0 bits (2083), Expect = 1.9e-232
Identity = 436/739 (59.00%), Postives = 537/739 (72.67%), Query Frame = 0 

Query: 34  SSLLKLPSQHSPETLTRPQLKALVLSHFSQGKFFDLFQNVVASPSVLLTASQNLISSPLS 93
           SSL     Q   E L + +L+ALVL  +S GKF+ L +N V+ P VLL A QNL  S   
Sbjct: 30  SSLFLNSDQTITEPLVKSELEALVLKQYSHGKFYSLVKNAVSLPCVLLAACQNLSLS--- 89

Query: 94  NAPDSLPVFELVSNCFSVEVMARELSENRFDVGACCVRMAPLEEKGESLVLPNLKLKVLI 153
            A  S  + + VS  FS+E M RE+ E RFD+ +CCV     E    SLVLPNLKLKVLI
Sbjct: 90  -ANSSGDLADRVSRRFSIEEMGREIREGRFDIRSCCV-----EFISSSLVLPNLKLKVLI 149

Query: 154 EAIRIVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKNSVQNPSWWFTVAFRHKKFESVHV 213
           EAIR+VLEIVYD+RF TFSYGGRVGMGRHTAIRYLKNSV+NP WWF V+F  + FE  +V
Sbjct: 150 EAIRMVLEIVYDDRFATFSYGGRVGMGRHTAIRYLKNSVENPRWWFRVSFAREMFEERNV 209

Query: 214 NTLCLLMQEKIKDDTLIYMLRKLFELEAIQIELGAFYLGRGFPQESSLCSILFNIYFNGF 273
           + LC  + EKI D  LI M++KLFE   ++IELG    GRGFPQE  LCSIL N+YF+G 
Sbjct: 210 DILCGFVGEKINDVMLIEMIKKLFEFGILKIELGGCNSGRGFPQECGLCSILINVYFDGL 269

Query: 274 DKEIQQIRLQKNEENPKFKLDEIVS----FHNPVKVYAVRYLDEILVITSGSKMLTMELK 333
           DKEIQ +RL+   +NP+    +  S    F  PV +YAVRYLDEILVITSGSKMLTM+LK
Sbjct: 270 DKEIQDLRLKMKVKNPRVGTGDEESTGNVFFKPVNIYAVRYLDEILVITSGSKMLTMDLK 329

Query: 334 SQVLKYLEGNLELEVDRMNTAIHSAVSEKISFLGMELQAVPPSVLHPPMSEKAVRARKKY 393
            +++  LE  LEL VDR+NT+IHSAVSEKI+FLGM LQAVPPSVL PP SEKAVRA KKY
Sbjct: 330 KRIVDILEQRLELRVDRLNTSIHSAVSEKINFLGMYLQAVPPSVLRPPKSEKAVRAMKKY 389

Query: 394 LRQKEVRAIELRNARERNRKKLGLKILSHVFKKLKQTSGFKSEFQIEKEVRDIFRNWADE 453
            RQK+VR +ELRNARERNRK LGLKI  HV KK+KQ++GFK E +IE EVRDIF++W +E
Sbjct: 390 QRQKDVRKLELRNARERNRKTLGLKIFRHVLKKIKQSNGFKFEGEIENEVRDIFQSWGEE 449

Query: 454 VVQDFFESSEDHAEWHRLLSAGDFLSLKHIRNQLPENLVNAYDRFQDQVNKHLNPVKVKK 513
           V+QDF  S E+  +WH LL+ GDFLSL+HIR +LP++L++AYD FQ+QV+KHL P + K 
Sbjct: 450 VMQDFMGSLEERWKWHWLLTRGDFLSLRHIREKLPQDLIDAYDEFQEQVDKHLAPTQAK- 509

Query: 514 EKAREDEEKRVEEEE--LYAKRTVEDLTRLCIKVDAPIELVRKAVKMVGFTNKMGRPRPI 573
            K  EDEE+RVEEEE   YA+RTVEDLT+LC+KV AP ELVRKA+K+VGFTN MGRPRPI
Sbjct: 510 -KVLEDEERRVEEEEEQRYAERTVEDLTKLCMKVSAPEELVRKAIKLVGFTNSMGRPRPI 569

Query: 574 SSLLALEDADIIKWYSGVGRRWLDFFCCCHNYKTVKTVVTYHLRFSCILTLAEKHESTKR 633
             L+ LED+DIIKWY+                                    EKH STK+
Sbjct: 570 IHLVTLEDSDIIKWYA----------------------------------RHEKHGSTKK 629

Query: 634 EAMKHYSKDLKVSDLNGNEEMHFPTEREVKMLGERNLVDPYPVDGALSLLLIRLATDEAS 693
             ++HY+KDL+VSDL+G EE HFP+EREVKM+G++NL DP PVDG LSLLLIRLA+DE  
Sbjct: 630 -LIRHYTKDLRVSDLDGREEAHFPSEREVKMMGDKNLSDPKPVDGTLSLLLIRLASDEPL 689

Query: 694 YPCIAHFCNRTDSILYRVRLLQRTLNVNPSNGVEWVRGMGVIHESLNQRCLPLCADHISD 753
           + C A FC R+D+I++RV LLQ  L++NP +  +WV GMG IH +LN++CLPLC+ HISD
Sbjct: 690 HHCAASFCERSDTIMHRVHLLQNRLHINPLDEEKWVPGMGTIHSALNRKCLPLCSTHISD 722

Query: 754 LYMGKINLQDLDCTLSLDM 767
           +Y+GKI LQD+D +  +D+
Sbjct: 750 VYLGKITLQDVDSSSFIDL 722

[Close]
BLAST of Lsi05G005990 vs. ExPASy Swiss-Prot
Match: Q9CA78 (Nuclear intron maturase 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NMAT4 PE=3 SV=2)

HSP 1 Score: 226.9 bits (577), Expect = 7.9e-58
Identity = 203/753 (26.96%), Postives = 338/753 (44.89%), Query Frame = 0 

Query: 52  QLKALVLSHFSQGKFFDLFQNVVASPSVLLTASQNL-ISSPLSNAPDSLPVFELVSNCFS 111
           +LK  V      GKF DL + V+A P  L  A   + ++S +S    +  V        +
Sbjct: 90  RLKKRVKEQCINGKFSDLLKKVIARPETLRDAYDCIRLNSNVSITERNGSV--------A 149

Query: 112 VEVMARELSENRFDVGACCVRMAPLEEKGESLVLPNLKLKVLIEAIRIVLEIVYDERFVT 171
            + +A ELS   FDV +    +   ++  E LVLP++ LKV+ EAIRIVLE+V+   F  
Sbjct: 150 FDSIAEELSSGVFDVASNTFSIVARDKTKEVLVLPSVALKVVQEAIRIVLEVVFSPHFSK 209

Query: 172 FSYGGRVGMGRHTAIRYLKNSVQNPSWWFTVAFRHKKFESVHVNTLCLLMQEKIKDDTLI 231
            S+  R G GR +A++Y+ N++    W FT++   K   SV  N L  +M+EK++D +L 
Sbjct: 210 ISHSCRSGRGRASALKYINNNISRSDWCFTLSLNKKLDVSVFENLLS-VMEEKVEDSSLS 269

Query: 232 YMLRKLFELEAIQIELGAFYLGRGFPQESSLCSILFNIYFNGFDKEIQQIRL-------- 291
            +LR +FE   + +E G F  G G PQE  L  +L NIY + FD E  +I +        
Sbjct: 270 ILLRSMFEARVLNLEFGGFPKGHGLPQEGVLSRVLMNIYLDRFDHEFYRISMRHEALGLD 329

Query: 292 -QKNEENPKFK-------------LDEIVSFHNPVKVYAVRYLDEILVITSGSKMLTMEL 351
            + +E++P  K             L         ++VY  R++DEI    SG K +  ++
Sbjct: 330 SKTDEDSPGSKLRSWFRRQAGEQGLKSTTEQDVALRVYCCRFMDEIYFSVSGPKKVASDI 389

Query: 352 KSQVLKYLEGNLELEVDRMNTAIHSAVSEKISFLGMELQAVPPSVLHPPMSEKAVRARKK 411
           +S+ + +L  +L L++           +  +  LG     V  +V   P + KAV   K+
Sbjct: 390 RSEAIGFLRNSLHLDITDETDPSPCEATSGLRVLG---TLVRKNVRESP-TVKAVHKLKE 449

Query: 412 YLRQKEVRAIELRNARERNRKKLGLKILSHVFKKLKQTS----------------GFKSE 471
            +R   + A++   A      ++G K L H  KK+K++                   K+ 
Sbjct: 450 KVR---LFALQKEEAWTLGTVRIGKKWLGHGLKKVKESEIKGLADSNSTLSQISCHRKAG 509

Query: 472 FQIEKEVRDIFRNWADEVVQDFFESSEDHAEWHRLLSAGDFLSLKH-IRNQLPENLVNAY 531
            + +   + + R W ++V++    +S D +E        +F+  KH +   +P+ L +A+
Sbjct: 510 METDHWYKILLRIWMEDVLR----TSADRSE--------EFVLSKHVVEPTVPQELRDAF 569

Query: 532 DRFQDQVNKHLNPVKVKKEKAREDEEKRVEEEELYAKRTVEDLTRLCIKVDAPIELVRKA 591
            +FQ+    +++              +    E L       D       V AP   + + 
Sbjct: 570 YKFQNAAAAYVS-------------SETANLEALLPCPQSHDRPVFFGDVVAPTNAIGRR 629

Query: 592 VKMVGFTNKMGRPRPISSLLALEDADIIKWYSGVGRRWLDFFCCCHNYKTVKTVVTYHLR 651
           +   G     G  R  S L+ L+ A II WYSG+ RRW+ ++  C N+  +K ++   +R
Sbjct: 630 LYRYGLITAKGYARSNSMLILLDTAQIIDWYSGLVRRWVIWYEGCSNFDEIKALIDNQIR 689

Query: 652 FSCILTLAEKHESTKREAMKHYSKDLKVSDLNGNEEMHFPTERE----VKMLGERNLVDP 711
            SCI TLA K+   + E  K    DL++S +   E++    + E         + +L   
Sbjct: 690 MSCIRTLAAKYRIHENEIEKRL--DLELSTIPSAEDIEQEIQHEKLDSPAFDRDEHLTYG 749

Query: 712 YPVDGALSLLLIRLATDEASYPCIAHFCNRTDSILYRVRLLQRTLNVNPSNGVEWVRGMG 760
               G   L L RL ++     C    C+     +Y +  ++R           W  G  
Sbjct: 750 LSNSGLCLLSLARLVSESRPCNCFVIGCSMAAPAVYTLHAMER------QKFPGWKTGFS 793

[Close]
BLAST of Lsi05G005990 vs. ExPASy Swiss-Prot
Match: B1N1A3 (Putative nicotine oxidoreductase OS=Pseudomonas putida OX=303 GN=nicA PE=4 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 1.7e-12
Identity = 70/264 (26.52%), Postives = 122/264 (46.21%), Query Frame = 0 

Query: 150 KVLIEAIRIVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKNSVQNPSWWFTVAFRHKKFE 209
           KV+ E IR +LE +Y+  F   S+G R G   HTA++ ++ S    +W      +   F+
Sbjct: 109 KVVQEVIRSILEAIYEPTFSKNSHGFRAGKSCHTALKQVRESWSGVTWVIEGDIK-GCFD 168

Query: 210 SVHVNTLCLLMQEKIKDDTLIYMLRKLFELEAIQIELGAFYLGR-GFPQESSLCSILFNI 269
           ++  + L   ++ +IKD+  I ++RK   L A   E GAF+    G PQ S +  IL N+
Sbjct: 169 NISHSKLIDQLRLRIKDERFINLIRK--ALNAGYFENGAFFSATLGTPQGSIISPILANV 228

Query: 270 YFNGFDKEIQQI-------------------RLQKNEENPKFKLDE-------------- 329
           + +  D++++Q+                   +LQ+ + + + K ++              
Sbjct: 229 FLDQLDRKVEQLIKDHHQGEEGDKITDPAYRKLQRQKTSLRKKAEKQEGAERDATLSLAR 288

Query: 330 ------------IVSFHNPVKVYAVRYLDEILVITSGSKMLTMELKSQVLKYLEGNLELE 368
                       +   +  ++V  VRY D+ ++  +G K+L  EL+S V ++LE N  LE
Sbjct: 289 EANSKLLSMSPYLTRNNGFIRVKYVRYADDWIIGVNGPKLLAEELRSVVGEFLE-NAGLE 348

[Close]
BLAST of Lsi05G005990 vs. ExPASy Swiss-Prot
Match: P03876 (Putative COX1/OXI3 intron 2 protein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=AI2 PE=4 SV=2)

HSP 1 Score: 73.2 bits (178), Expect = 1.5e-11
Identity = 66/262 (25.19%), Postives = 117/262 (44.66%), Query Frame = 0 

Query: 142 LVLPNLKLKVLIEAIRIVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKNSVQNPSWWFTV 201
           L + N + K++ E++R++LEI+Y+  F  +S+G R  +   TAI   KN +Q  +W+  V
Sbjct: 358 LSVGNPREKIVQESMRMMLEIIYNNSFSYYSHGFRPNLSCLTAIIQCKNYMQYCNWFIKV 417

Query: 202 AFRHKKFESVHVNTLCLLMQEKIKDDTLIYMLRKLFELEAIQIELGAFYLGRGFPQESSL 261
              +K F+++  N L  ++ E+IKD   + +L KL     +           G PQ S +
Sbjct: 418 DL-NKCFDTIPHNMLINVLNERIKDKGFMDLLYKLLRAGYVDKNNNYHNTTLGIPQGSVV 477

Query: 262 CSILFNIYF--------NGFDKEIQQIRLQKNEENP-----------------KFKLDEI 321
             IL NI+         N F+ E     +     NP                 K KL  +
Sbjct: 478 SPILCNIFLDKLDKYLENKFENEFNTGNMSNRGRNPIYNSLSSKIYRCKLLSEKLKLIRL 537

Query: 322 VSFHNP--------VKVYAVRYLDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRMN 371
              +           + Y VRY D+I++   GS      + + +  +L+ NL + ++ M+
Sbjct: 538 RDHYQRNMGSDKSFKRAYFVRYADDIIIGVMGSHNDCKNILNDINNFLKENLGMSIN-MD 597

[Close]
BLAST of Lsi05G005990 vs. ExPASy Swiss-Prot
Match: P0A3U0 (Group II intron-encoded protein LtrA OS=Lactococcus lactis subsp. cremoris OX=1359 GN=ltrA PE=1 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 5.6e-11
Identity = 72/275 (26.18%), Postives = 115/275 (41.82%), Query Frame = 0 

Query: 137 EKGESLVLPNLKLKVLIEAIRIVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKNSVQNPS 196
           +K   L +P    K++ EA+RI+LE +Y+  F   S+G R     HTA++ +K      +
Sbjct: 95  KKMRPLGIPTFTDKLIQEAVRIILESIYEPVFEDVSHGFRPQRSCHTALKTIKREF-GGA 154

Query: 197 WWFTVAFRHKKFESVHVNTLCLLMQEKIKD---DTLIYMLRKLFELEAIQIELGAFYLGR 256
            WF        F+++   TL  L+  KIKD     LIY   K   LE  Q          
Sbjct: 155 RWFVEGDIKGCFDNIDHVTLIGLINLKIKDMKMSQLIYKFLKAGYLENWQYH----KTYS 214

Query: 257 GFPQESSLCSILFNIYFNGFDKEIQQIRLQKNEENPKFKLDEIVSFHNPVK--------- 316
           G PQ   L  +L NIY +  DK + Q++++ + E+P+    E    HN +K         
Sbjct: 215 GTPQGGILSPLLANIYLHELDKFVLQLKMKFDRESPERITPEYRELHNEIKRISHRLKKL 274

Query: 317 -------------------------------VYAVRYLDEILVITSGSKMLTMELKSQVL 368
                                          +  VRY D+ ++   GSK     +K Q+ 
Sbjct: 275 EGEEKAKVLLEYQEKRKRLPTLPCTSQTNKVLKYVRYADDFIISVKGSKEDCQWIKEQLK 334

[Close]
BLAST of Lsi05G005990 vs. ExPASy TrEMBL
Match: A0A1S4E4Y1 (uncharacterized protein LOC103501588 OS=Cucumis melo OX=3656 GN=LOC103501588 PE=4 SV=1)

HSP 1 Score: 1382.1 bits (3576), Expect = 0.0e+00
Identity = 704/767 (91.79%), Postives = 728/767 (94.92%), Query Frame = 0 

Query: 1   MFIQFRKIATPLKPKFSNSLTKPYFHLPLKLKHSSLLKLPSQHSPETLTRPQLKALVLSH 60
           MFI FRKIAT LKPKFSNSL K Y HLPL+ K   +L LPSQHSPE+LT  QLKALVLS 
Sbjct: 1   MFIHFRKIATSLKPKFSNSLNKLYSHLPLRKK---VLNLPSQHSPESLTSSQLKALVLSR 60

Query: 61  FSQGKFFDLFQNVVASPSVLLTASQNLISSPLSNAPDSLPVFELVSNCFSVEVMARELSE 120
           FS GKF DLFQNVVASPSVLLTAS+NLI+ P SNAPDSLP+ +LVS CFSVEVMARELSE
Sbjct: 61  FSHGKFVDLFQNVVASPSVLLTASRNLITPPFSNAPDSLPLSDLVSKCFSVEVMARELSE 120

Query: 121 NRFDVGACCVRMAPLEEKGESLVLPNLKLKVLIEAIRIVLEIVYDERFVTFSYGGRVGMG 180
           NRFDVGACCV MAPLEEKGESLVLPNLKLKVLIEAIR+VLEIVYDERFVTFSYGGRVGMG
Sbjct: 121 NRFDVGACCVPMAPLEEKGESLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGMG 180

Query: 181 RHTAIRYLKNSVQNPSWWFTVAFRHKKFESVHVNTLCLLMQEKIKDDTLIYMLRKLFELE 240
           RHTAIRYLKNSVQNPSWWFTVAFR KKFESVHVNTLCLLMQEKIKDD LIYMLRKLFE+E
Sbjct: 181 RHTAIRYLKNSVQNPSWWFTVAFRRKKFESVHVNTLCLLMQEKIKDDILIYMLRKLFEVE 240

Query: 241 AIQIELGAFYLGRGFPQESSLCSILFNIYFNGFDKEIQQIRLQKNEENPKFKLDEIVSFH 300
           AIQIELG  YLGRGFPQES LCSIL NIYFNGFDKEIQ+IRLQKNEENPKF LDEIVSFH
Sbjct: 241 AIQIELGGCYLGRGFPQESGLCSILLNIYFNGFDKEIQRIRLQKNEENPKFNLDEIVSFH 300

Query: 301 NPVKVYAVRYLDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRMNTAIHSAVSEKIS 360
           NPVK+YAVRYLDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRMNTAIHSAVSEKI 
Sbjct: 301 NPVKIYAVRYLDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRMNTAIHSAVSEKIG 360

Query: 361 FLGMELQAVPPSVLHPPMSEKAVRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVF 420
           FLGMELQAV PSVLHPPMSEKA+RARKKYLRQKEVRAIELRNARERNRKKLGLKILSH+F
Sbjct: 361 FLGMELQAVTPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHMF 420

Query: 421 KKLKQTSGFKSEFQIEKEVRDIFRNWADEVVQDFFESSEDHAEWHRLLSAGDFLSLKHIR 480
           KK K+T GFKSEFQIE EVR IFRNWADEVVQDFFESSEDHAEWHR+LSAGDFLSLKHIR
Sbjct: 421 KKFKRTGGFKSEFQIETEVRSIFRNWADEVVQDFFESSEDHAEWHRVLSAGDFLSLKHIR 480

Query: 481 NQLPENLVNAYDRFQDQVNKHLNPVKVKKEKAREDEEKRVEEEELYAKRTVEDLTRLCIK 540
           NQLPE+LVNAYDRFQ QVNKHLNPVKVKKEKAREDEEKR+EEEELYAKRTVEDLTRLCIK
Sbjct: 481 NQLPEDLVNAYDRFQYQVNKHLNPVKVKKEKAREDEEKRLEEEELYAKRTVEDLTRLCIK 540

Query: 541 VDAPIELVRKAVKMVGFTNKMGRPRPISSLLALEDADIIKWYSGVGRRWLDFFCCCHNYK 600
           VDAPIELVRKAV+MVGFTNKMGRPRPISSL+ALEDADIIKWYSGVGRRWLDFFCCCHNYK
Sbjct: 541 VDAPIELVRKAVRMVGFTNKMGRPRPISSLIALEDADIIKWYSGVGRRWLDFFCCCHNYK 600

Query: 601 TVKTVVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVSDLNGNEEMHFPTEREVKMLG 660
            VKTVVTYHLRFSCILTLAEKHESTKREAMKHY KDLKV DLNGNEEMHFPTE+ VKMLG
Sbjct: 601 MVKTVVTYHLRFSCILTLAEKHESTKREAMKHYGKDLKVFDLNGNEEMHFPTEKAVKMLG 660

Query: 661 ERNLVDPYPVDGALSLLLIRLATDEASYPCIAHFCNRTDSILYRVRLLQRTLNVNPSNGV 720
           ERNL DPYPVDGALSLLLIRLATDEASYPCIA+FCNRT+SILYRVRLLQRTLNVNPS+GV
Sbjct: 661 ERNLADPYPVDGALSLLLIRLATDEASYPCIANFCNRTNSILYRVRLLQRTLNVNPSDGV 720

Query: 721 EWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 768
           EWV+GMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD
Sbjct: 721 EWVKGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 764

[Close]
BLAST of Lsi05G005990 vs. ExPASy TrEMBL
Match: A0A5A7SWY5 (RNA-directed DNA polymerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold400G00320 PE=4 SV=1)

HSP 1 Score: 1379.4 bits (3569), Expect = 0.0e+00
Identity = 704/767 (91.79%), Postives = 727/767 (94.78%), Query Frame = 0 

Query: 1   MFIQFRKIATPLKPKFSNSLTKPYFHLPLKLKHSSLLKLPSQHSPETLTRPQLKALVLSH 60
           MFI FRKIAT LKPKFSNSL K Y HLPL+ K   +L LPSQHSPE+LT  QLKALVLS 
Sbjct: 1   MFIHFRKIATSLKPKFSNSLNKLYSHLPLRKK---VLNLPSQHSPESLTSSQLKALVLSR 60

Query: 61  FSQGKFFDLFQNVVASPSVLLTASQNLISSPLSNAPDSLPVFELVSNCFSVEVMARELSE 120
           FS GKF DLFQNVVASPSVLLTAS+NLI+ P SNAPDSLP+ +LVS CFSVEVMARELSE
Sbjct: 61  FSHGKFVDLFQNVVASPSVLLTASRNLITPPFSNAPDSLPLSDLVSKCFSVEVMARELSE 120

Query: 121 NRFDVGACCVRMAPLEEKGESLVLPNLKLKVLIEAIRIVLEIVYDERFVTFSYGGRVGMG 180
           NRFDVGACCV MAPLEEKGESLVLPNLKLKVLIEAIR+VLEIVYDERFVTFSYGGRVGMG
Sbjct: 121 NRFDVGACCVPMAPLEEKGESLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGMG 180

Query: 181 RHTAIRYLKNSVQNPSWWFTVAFRHKKFESVHVNTLCLLMQEKIKDDTLIYMLRKLFELE 240
           RHTAIRYLKNSVQNPSWWFTVAFR KKFESVHVNTLCLLMQEKIKDD LIYMLRKLFE+E
Sbjct: 181 RHTAIRYLKNSVQNPSWWFTVAFRRKKFESVHVNTLCLLMQEKIKDDILIYMLRKLFEVE 240

Query: 241 AIQIELGAFYLGRGFPQESSLCSILFNIYFNGFDKEIQQIRLQKNEENPKFKLDEIVSFH 300
           AIQIELG  YLGRGFPQES LCSIL NIYFNGFDKEIQ+IRLQKNEENPKF LDEIVSFH
Sbjct: 241 AIQIELGGCYLGRGFPQESGLCSILLNIYFNGFDKEIQRIRLQKNEENPKFNLDEIVSFH 300

Query: 301 NPVKVYAVRYLDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRMNTAIHSAVSEKIS 360
           NPVK+YAVRYLDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRMNTAIHSAVSEKI 
Sbjct: 301 NPVKIYAVRYLDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRMNTAIHSAVSEKIG 360

Query: 361 FLGMELQAVPPSVLHPPMSEKAVRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVF 420
           FLGMELQAV PSVLHPPMSEKA+RARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVF
Sbjct: 361 FLGMELQAVTPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVF 420

Query: 421 KKLKQTSGFKSEFQIEKEVRDIFRNWADEVVQDFFESSEDHAEWHRLLSAGDFLSLKHIR 480
           KK K+T GFKSEFQIE EVR IFRNWADEVVQDFFESSED AEWHR+LSAGDFLSLKHIR
Sbjct: 421 KKFKRTGGFKSEFQIETEVRSIFRNWADEVVQDFFESSEDPAEWHRVLSAGDFLSLKHIR 480

Query: 481 NQLPENLVNAYDRFQDQVNKHLNPVKVKKEKAREDEEKRVEEEELYAKRTVEDLTRLCIK 540
           NQLPE+LVNAYDRFQ QVNKHLNPVKVKKEKAREDEEKR+EEEELYAKRTVEDLTRLCIK
Sbjct: 481 NQLPEDLVNAYDRFQYQVNKHLNPVKVKKEKAREDEEKRLEEEELYAKRTVEDLTRLCIK 540

Query: 541 VDAPIELVRKAVKMVGFTNKMGRPRPISSLLALEDADIIKWYSGVGRRWLDFFCCCHNYK 600
           VDAPIELVRKAV+MVGFTNKMGRPRPISSL+ALEDADIIKWYSGVGRRWLDFFCCCHNYK
Sbjct: 541 VDAPIELVRKAVRMVGFTNKMGRPRPISSLIALEDADIIKWYSGVGRRWLDFFCCCHNYK 600

Query: 601 TVKTVVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVSDLNGNEEMHFPTEREVKMLG 660
            VKTVVTYHLRFSCILTLAEKHESTKREAMKHY KDLKV DLNGNEEMHFPTE+ VKMLG
Sbjct: 601 MVKTVVTYHLRFSCILTLAEKHESTKREAMKHYGKDLKVFDLNGNEEMHFPTEKAVKMLG 660

Query: 661 ERNLVDPYPVDGALSLLLIRLATDEASYPCIAHFCNRTDSILYRVRLLQRTLNVNPSNGV 720
           ERNL DPYPVDGALSLLLIRLATDEASYPCIA+FCNRT+SILYRVRLLQRTLNVNPS+GV
Sbjct: 661 ERNLADPYPVDGALSLLLIRLATDEASYPCIANFCNRTNSILYRVRLLQRTLNVNPSDGV 720

Query: 721 EWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 768
           EWV+GMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD
Sbjct: 721 EWVKGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 764

[Close]
BLAST of Lsi05G005990 vs. ExPASy TrEMBL
Match: A0A6J1ILC2 (nuclear intron maturase 3, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111476629 PE=4 SV=1)

HSP 1 Score: 1311.6 bits (3393), Expect = 0.0e+00
Identity = 670/770 (87.01%), Postives = 706/770 (91.69%), Query Frame = 0 

Query: 1   MFIQFRKIATPLKPKFSNSLTKPYFHLPLKLKHSSLLKLPSQHSPETLTRPQLKALVLSH 60
           M I  RKIATPLKPK S+SL K Y   P  LKHS LLKLP QHS +TLTRPQL+ALVLS 
Sbjct: 1   MLIHLRKIATPLKPKLSSSLNKLYSDRP--LKHSPLLKLPFQHSVQTLTRPQLEALVLSR 60

Query: 61  FSQGKFFDLFQNVVASPSVLLTASQNLIS---SPLSNAPDSLPVFELVSNCFSVEVMARE 120
           FSQGKFFDL QNVVASPSVL TASQNLI+   S   NAP+SL   ++VS+CFSVE MARE
Sbjct: 61  FSQGKFFDLLQNVVASPSVLFTASQNLITPLPSNRLNAPESLLSLDMVSSCFSVEDMARE 120

Query: 121 LSENRFDVGACCVRMAPLEEKGESLVLPNLKLKVLIEAIRIVLEIVYDERFVTFSYGGRV 180
           L ENRFDVGACCVR+   EEKGE LVLPNLKLKVL+EA+++VLEIVYDERFVTFSYGGRV
Sbjct: 121 LYENRFDVGACCVRLESSEEKGEFLVLPNLKLKVLLEAMKMVLEIVYDERFVTFSYGGRV 180

Query: 181 GMGRHTAIRYLKNSVQNPSWWFTVAFRHKKFESVHVNTLCLLMQEKIKDDTLIYMLRKLF 240
           GMGRHTAIRYLKNSVQNPSWWFTVAFR +KF+SVHVN LCLL+QEKIKDD LI ML+KLF
Sbjct: 181 GMGRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLF 240

Query: 241 ELEAIQIELGAFYLGRGFPQESSLCSILFNIYFNGFDKEIQQIRLQKNEENPKFKLDEIV 300
           ELEA+QIELG  YLGRGFPQES LCSIL NIYFNGFDKEIQQIRLQKNEENPKF L+  V
Sbjct: 241 ELEAVQIELGGCYLGRGFPQESGLCSILSNIYFNGFDKEIQQIRLQKNEENPKFSLNGTV 300

Query: 301 SFHNPVKVYAVRYLDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRMNTAIHSAVSE 360
           SFHNPVK+YAVRYLDEILVITSGSKM  MELKSQVL+YLEGNLELEVDRMNTAIHSAVSE
Sbjct: 301 SFHNPVKIYAVRYLDEILVITSGSKMQIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSE 360

Query: 361 KISFLGMELQAVPPSVLHPPMSEKAVRARKKYLRQKEVRAIELRNARERNRKKLGLKILS 420
           KISFLGMELQAVPPSVLHPPMSEKA+RARKKYLRQKEVRAIELRNARERNRKKLGLKILS
Sbjct: 361 KISFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILS 420

Query: 421 HVFKKLKQTSGFKSEFQIEKEVRDIFRNWADEVVQDFFESSEDHAEWHRLLSAGDFLSLK 480
           HVFKKLK+T G KSEFQIEKEV +IFRNWADEVV+DFFES ED+ EWHR LSAGDFLSLK
Sbjct: 421 HVFKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLK 480

Query: 481 HIRNQLPENLVNAYDRFQDQVNKHLNPVKVKKEKAREDEEKRVEEEELYAKRTVEDLTRL 540
           HIRNQLP +LVNAYDRFQDQVNKHLNP+K KKEKAREDEEKR+ EEE YAKRTVEDLTRL
Sbjct: 481 HIRNQLPVDLVNAYDRFQDQVNKHLNPLKAKKEKAREDEEKRLVEEERYAKRTVEDLTRL 540

Query: 541 CIKVDAPIELVRKAVKMVGFTNKMGRPRPISSLLALEDADIIKWYSGVGRRWLDFFCCCH 600
           CIKV+APIELVRKAVKM+GFTNKMGRP+PISSL+ALED DIIKWY+GVGRRWLDFFCCCH
Sbjct: 541 CIKVEAPIELVRKAVKMIGFTNKMGRPQPISSLIALEDTDIIKWYAGVGRRWLDFFCCCH 600

Query: 601 NYKTVKTVVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVSDLNGNEEMHFPTEREVK 660
           NYK VKT+VTYHLRFSCILTLAEKHESTKREAMKHYSKDLKV DLNG EEMHFPTEREVK
Sbjct: 601 NYKMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVK 660

Query: 661 MLGERNLVDPYPVDGALSLLLIRLATDEASYPCIAHFCNRTDSILYRVRLLQRTLNVNPS 720
           MLGERNL DPYPVDGA SL LIRL TDE SYPCIAHFCNRTDSILYRVRLLQ+TLNVNPS
Sbjct: 661 MLGERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPS 720

Query: 721 NGVEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 768
           NGVEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD
Sbjct: 721 NGVEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 768

[Close]
BLAST of Lsi05G005990 vs. ExPASy TrEMBL
Match: A0A6J1FV64 (nuclear intron maturase 3, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111447574 PE=4 SV=1)

HSP 1 Score: 1310.4 bits (3390), Expect = 0.0e+00
Identity = 671/770 (87.14%), Postives = 704/770 (91.43%), Query Frame = 0 

Query: 1   MFIQFRKIATPLKPKFSNSLTKPYFHLPLKLKHSSLLKLPSQHSPETLTRPQLKALVLSH 60
           M I  RKIATPLKPK S+S  K Y   P  LKHS LLKLP QHS +TLTRPQL+ALVLS 
Sbjct: 1   MLIHLRKIATPLKPKLSSSFNKLYSDRP--LKHSPLLKLPFQHSVQTLTRPQLEALVLSR 60

Query: 61  FSQGKFFDLFQNVVASPSVLLTASQNLIS---SPLSNAPDSLPVFELVSNCFSVEVMARE 120
           FSQGKFFDL QNVVASPSVL TASQNLI+   S   NAPDSL   ++VS+CFSVE MARE
Sbjct: 61  FSQGKFFDLLQNVVASPSVLFTASQNLITPLPSNRLNAPDSLLSLDMVSSCFSVEDMARE 120

Query: 121 LSENRFDVGACCVRMAPLEEKGESLVLPNLKLKVLIEAIRIVLEIVYDERFVTFSYGGRV 180
           L ENRFDVGACCVR+   EEKGE LVLPNLKLKVL+EAIRIVLEIVYDERFVTFSYGGRV
Sbjct: 121 LYENRFDVGACCVRLESSEEKGEFLVLPNLKLKVLLEAIRIVLEIVYDERFVTFSYGGRV 180

Query: 181 GMGRHTAIRYLKNSVQNPSWWFTVAFRHKKFESVHVNTLCLLMQEKIKDDTLIYMLRKLF 240
           GMGRHTAIRYLKNSVQNPSWWFTVAFR +KF+SVHVN LCLL+QEKIKDD LI ML+KLF
Sbjct: 181 GMGRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLF 240

Query: 241 ELEAIQIELGAFYLGRGFPQESSLCSILFNIYFNGFDKEIQQIRLQKNEENPKFKLDEIV 300
           ELEA+QIELG  YLGRG PQES LCSIL NIYFNGFDKEIQQIRL+KNEENPKF LD  V
Sbjct: 241 ELEAVQIELGGCYLGRGLPQESGLCSILSNIYFNGFDKEIQQIRLEKNEENPKFSLDGTV 300

Query: 301 SFHNPVKVYAVRYLDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRMNTAIHSAVSE 360
           SFHNPVK+YAVRYLDEILVITSGSKML MELKSQVL+YLEGNLELEVDRMNTAIHSAVSE
Sbjct: 301 SFHNPVKIYAVRYLDEILVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSE 360

Query: 361 KISFLGMELQAVPPSVLHPPMSEKAVRARKKYLRQKEVRAIELRNARERNRKKLGLKILS 420
           KISFLGMELQAV PSVLHPPMSEKA+RARKKYLRQKEVRAIELRNARERNRKKLGLKILS
Sbjct: 361 KISFLGMELQAVLPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILS 420

Query: 421 HVFKKLKQTSGFKSEFQIEKEVRDIFRNWADEVVQDFFESSEDHAEWHRLLSAGDFLSLK 480
           HVFKKLK+T G KSEFQIEKEV +IFRNWADEVV+DFFES ED+ EWHR LSAGDFLSLK
Sbjct: 421 HVFKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLK 480

Query: 481 HIRNQLPENLVNAYDRFQDQVNKHLNPVKVKKEKAREDEEKRVEEEELYAKRTVEDLTRL 540
           HIRNQLP +LVNAYDRFQDQVN+HLNP+K K+EKAREDEEKR+ EEE YAKRTVEDLTRL
Sbjct: 481 HIRNQLPVDLVNAYDRFQDQVNQHLNPLKAKEEKAREDEEKRLGEEERYAKRTVEDLTRL 540

Query: 541 CIKVDAPIELVRKAVKMVGFTNKMGRPRPISSLLALEDADIIKWYSGVGRRWLDFFCCCH 600
           CIKV+APIELVRKAVKM+GFTNKMGRPRPISSL+ALED DIIKWY+GVGRRWLDFFCCCH
Sbjct: 541 CIKVEAPIELVRKAVKMIGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCH 600

Query: 601 NYKTVKTVVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVSDLNGNEEMHFPTEREVK 660
           NYK VKT+VTYHLRFSCILTLAEKHESTKREAMKHYSKDLKV DLNG EEMHFPTEREVK
Sbjct: 601 NYKMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVK 660

Query: 661 MLGERNLVDPYPVDGALSLLLIRLATDEASYPCIAHFCNRTDSILYRVRLLQRTLNVNPS 720
           MLGERNL DPYPVDGA SL LIRL TDE SYPCIAHFCNRTDSILYRVRLLQ+TLNVNPS
Sbjct: 661 MLGERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPS 720

Query: 721 NGVEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 768
           NGVEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD
Sbjct: 721 NGVEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 768

[Close]
BLAST of Lsi05G005990 vs. ExPASy TrEMBL
Match: A0A6J1DNH6 (nuclear intron maturase 3, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111022853 PE=4 SV=1)

HSP 1 Score: 1277.3 bits (3304), Expect = 0.0e+00
Identity = 660/772 (85.49%), Postives = 698/772 (90.41%), Query Frame = 0 

Query: 1   MFIQFRKIATPLKPKFSNSLTKPYFHLPLKLKHS-SLLKLPSQHSPETLTRPQLKALVLS 60
           MFI  RKIATPLK   S S  K Y  LP  LKHS  L   P QHS ETLT P+LKALVLS
Sbjct: 1   MFIPLRKIATPLKSILSTSFNKLYSDLP--LKHSPPLHNFPFQHSTETLTWPELKALVLS 60

Query: 61  HFSQGKFFDLFQNVVASPSVLLTASQNLISSPLS----NAPDSLPVFELVSNCFSVEVMA 120
            F+ GKF DL QNVVASPSVLLTASQNLI+ P      NAPD LP+ +LVS CFSVE MA
Sbjct: 61  RFTHGKFLDLLQNVVASPSVLLTASQNLITPPPPSNGLNAPDPLPILDLVSKCFSVEEMA 120

Query: 121 RELSENRFDVGACCVRMAPLEEKGESLVLPNLKLKVLIEAIRIVLEIVYDERFVTFSYGG 180
           REL E+RFDVGACCVRM   ++KG+SLVLPNLKLKVLIEAIR+VLEIVYDERFVTFSYGG
Sbjct: 121 RELYEDRFDVGACCVRM---DQKGQSLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGG 180

Query: 181 RVGMGRHTAIRYLKNSVQNPSWWFTVAFRHKKFESVHVNTLCLLMQEKIKDDTLIYMLRK 240
           RVGMGRHTAIRYLKNSVQNPSWWFTVAFR KKF+SVHV+ LCLL++EKIKD  LI ML+K
Sbjct: 181 RVGMGRHTAIRYLKNSVQNPSWWFTVAFRRKKFDSVHVHKLCLLVEEKIKDHLLICMLKK 240

Query: 241 LFELEAIQIELGAFYLGRGFPQESSLCSILFNIYFNGFDKEIQQIRLQKNEENPKFKLDE 300
           LFELEAIQIELGA YLGRGFPQES LCSIL NI+FNGFDK+IQQIRLQKNEENPKF LDE
Sbjct: 241 LFELEAIQIELGACYLGRGFPQESGLCSILCNIFFNGFDKDIQQIRLQKNEENPKFSLDE 300

Query: 301 IVSFHNPVKVYAVRYLDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRMNTAIHSAV 360
           IVSFH+PVK+YAVRYLDEILVITSGSKMLTM+LKSQVLKYLEGNLELEVDRMNTAIHSAV
Sbjct: 301 IVSFHSPVKIYAVRYLDEILVITSGSKMLTMDLKSQVLKYLEGNLELEVDRMNTAIHSAV 360

Query: 361 SEKISFLGMELQAVPPSVLHPPMSEKAVRARKKYLRQKEVRAIELRNARERNRKKLGLKI 420
           SEKISFLGMELQAVPPSVLHPPMSEKA+RARKKY+RQKEVR IELRNARERNRKKLGLKI
Sbjct: 361 SEKISFLGMELQAVPPSVLHPPMSEKAIRARKKYIRQKEVRVIELRNARERNRKKLGLKI 420

Query: 421 LSHVFKKLKQTSGFKSEFQIEKEVRDIFRNWADEVVQDFFESSEDHAEWHRLLSAGDFLS 480
           LSHVFKKLKQT GFK EFQIEKEVR+IFRNWADEV Q FFES E+HAEWH  LSAGDFLS
Sbjct: 421 LSHVFKKLKQTDGFKFEFQIEKEVREIFRNWADEVAQHFFESLENHAEWHHALSAGDFLS 480

Query: 481 LKHIRNQLPENLVNAYDRFQDQVNKHLNPVKVKKEKAREDEEKRVEEEELYAKRTVEDLT 540
           LKHIRNQLPE+LVNAYDRFQDQV+KHLNPVKVK  KAREDEEKRVEEE+ YA+RTVEDLT
Sbjct: 481 LKHIRNQLPEDLVNAYDRFQDQVDKHLNPVKVKYVKAREDEEKRVEEEQKYARRTVEDLT 540

Query: 541 RLCIKVDAPIELVRKAVKMVGFTNKMGRPRPISSLLALEDADIIKWYSGVGRRWLDFFCC 600
           RLCIKVDAPIELVRKAVKMVGFTNKMGRP+PIS L+ALED DIIKWY+GVGRRWLDFFCC
Sbjct: 541 RLCIKVDAPIELVRKAVKMVGFTNKMGRPQPISLLVALEDIDIIKWYAGVGRRWLDFFCC 600

Query: 601 CHNYKTVKTVVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVSDLNGNEEMHFPTERE 660
           CHNYK VKTVVTYHLRFSCILTLAEKHESTKREAMKH+SKDLKV DLNG+EE+HFPTERE
Sbjct: 601 CHNYKMVKTVVTYHLRFSCILTLAEKHESTKREAMKHFSKDLKVFDLNGDEEIHFPTERE 660

Query: 661 VKMLGERNLVDPYPVDGALSLLLIRLATDEASYPCIAHFCNRTDSILYRVRLLQRTLNVN 720
           VKMLG+R L DPYPVDG LSL LIRLA DE S PCIAHFCNRTDSILYRVRLLQRTLNVN
Sbjct: 661 VKMLGDRMLADPYPVDGTLSLFLIRLAIDEPSCPCIAHFCNRTDSILYRVRLLQRTLNVN 720

Query: 721 PSNGVEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 768
             +G +WVRGMGVIHESLNQRCLPLCADHISDLYMG+INLQDLDCTLSLDMD
Sbjct: 721 SCDGEKWVRGMGVIHESLNQRCLPLCADHISDLYMGRINLQDLDCTLSLDMD 767

[Close]
BLAST of Lsi05G005990 vs. NCBI nr
Match: XP_038904122.1 (nuclear intron maturase 3, mitochondrial [Benincasa hispida])

HSP 1 Score: 1420.6 bits (3676), Expect = 0.0e+00
Identity = 721/767 (94.00%), Postives = 740/767 (96.48%), Query Frame = 0 

Query: 1   MFIQFRKIATPLKPKFSNSLTKPYFHLPLKLKHSSLLKLPSQHSPETLTRPQLKALVLSH 60
           MFI FRK+ TPLKPK SNSLTK Y HLPLKLKHS LL LPSQHSPETLTR QLKALVLS 
Sbjct: 73  MFIHFRKVVTPLKPKLSNSLTKLYSHLPLKLKHSPLLNLPSQHSPETLTRSQLKALVLSR 132

Query: 61  FSQGKFFDLFQNVVASPSVLLTASQNLISSPLSNAPDSLPVFELVSNCFSVEVMARELSE 120
           FSQGKFFDLFQNVVASPSVLLTASQNLI+SPLSNAPDSLP+F+LVSNCFSVEVMARELSE
Sbjct: 133 FSQGKFFDLFQNVVASPSVLLTASQNLITSPLSNAPDSLPLFDLVSNCFSVEVMARELSE 192

Query: 121 NRFDVGACCVRMAPLEEKGESLVLPNLKLKVLIEAIRIVLEIVYDERFVTFSYGGRVGMG 180
           NRFDVGACCVRM  LE KGESLVLPNLKLKVLIEAIR+V EIVYDERFVTFSYGGRVGMG
Sbjct: 193 NRFDVGACCVRMVSLEGKGESLVLPNLKLKVLIEAIRMVFEIVYDERFVTFSYGGRVGMG 252

Query: 181 RHTAIRYLKNSVQNPSWWFTVAFRHKKFESVHVNTLCLLMQEKIKDDTLIYMLRKLFELE 240
           RHTAIRYLKNSVQNPSWWFTVAFRHKKFESVHV+TLCLLM+EKIKDD LI MLRKLFELE
Sbjct: 253 RHTAIRYLKNSVQNPSWWFTVAFRHKKFESVHVDTLCLLMEEKIKDDILINMLRKLFELE 312

Query: 241 AIQIELGAFYLGRGFPQESSLCSILFNIYFNGFDKEIQQIRLQKNEENPKFKLDEIVSFH 300
           AIQIELGA YLGRG PQESSLCSILFNIYFNGFDKEIQQIRLQKNEENPKF LDEIVSFH
Sbjct: 313 AIQIELGACYLGRGLPQESSLCSILFNIYFNGFDKEIQQIRLQKNEENPKFSLDEIVSFH 372

Query: 301 NPVKVYAVRYLDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRMNTAIHSAVSEKIS 360
           NPVK+YAVRYLDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRMNTAIHSAVSEKIS
Sbjct: 373 NPVKIYAVRYLDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRMNTAIHSAVSEKIS 432

Query: 361 FLGMELQAVPPSVLHPPMSEKAVRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVF 420
           FLGMELQAV PSVLHPPMSEKA+RARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVF
Sbjct: 433 FLGMELQAVAPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVF 492

Query: 421 KKLKQTSGFKSEFQIEKEVRDIFRNWADEVVQDFFESSEDHAEWHRLLSAGDFLSLKHIR 480
           KKLKQTSGFKSEF+IEKEVRDIFR WA+EVVQDFF+S EDHAEWHR LSAGDFLSLKHIR
Sbjct: 493 KKLKQTSGFKSEFRIEKEVRDIFRKWANEVVQDFFQSFEDHAEWHRELSAGDFLSLKHIR 552

Query: 481 NQLPENLVNAYDRFQDQVNKHLNPVKVKKEKAREDEEKRVEEEELYAKRTVEDLTRLCIK 540
           NQLPE+LVNAYDRFQDQ++KHLNPVKVKKEKAREDEEKRVEEEELYAKRTVEDLTRLCIK
Sbjct: 553 NQLPEDLVNAYDRFQDQIDKHLNPVKVKKEKAREDEEKRVEEEELYAKRTVEDLTRLCIK 612

Query: 541 VDAPIELVRKAVKMVGFTNKMGRPRPISSLLALEDADIIKWYSGVGRRWLDFFCCCHNYK 600
           VDAPIELVRKAVKMVGFTNKMGRPRPISSL+ALED DIIKWYSGVGRRWLDFF CCHNYK
Sbjct: 613 VDAPIELVRKAVKMVGFTNKMGRPRPISSLIALEDTDIIKWYSGVGRRWLDFFSCCHNYK 672

Query: 601 TVKTVVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVSDLNGNEEMHFPTEREVKMLG 660
            VKTVVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKV DLNGNEEMHFPTEREVKMLG
Sbjct: 673 MVKTVVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVYDLNGNEEMHFPTEREVKMLG 732

Query: 661 ERNLVDPYPVDGALSLLLIRLATDEASYPCIAHFCNRTDSILYRVRLLQRTLNVNPSNGV 720
           ERNLVDPYPVDGALSLLLIRLATDEASYPCIAHFCNRTDS+LYRVRLLQRTLNVNP NGV
Sbjct: 733 ERNLVDPYPVDGALSLLLIRLATDEASYPCIAHFCNRTDSVLYRVRLLQRTLNVNPLNGV 792

Query: 721 EWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 768
           EWV+G+GVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD
Sbjct: 793 EWVKGLGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 839

[Close]
BLAST of Lsi05G005990 vs. NCBI nr
Match: XP_031738720.1 (nuclear intron maturase 3, mitochondrial [Cucumis sativus])

HSP 1 Score: 1401.3 bits (3626), Expect = 0.0e+00
Identity = 710/767 (92.57%), Postives = 732/767 (95.44%), Query Frame = 0 

Query: 1   MFIQFRKIATPLKPKFSNSLTKPYFHLPLKLKHSSLLKLPSQHSPETLTRPQLKALVLSH 60
           MFI FRKIAT LKPKFSNSL K Y HLPLKLKHS  L LPSQHSPETLT  QLKALVLS 
Sbjct: 48  MFIHFRKIATSLKPKFSNSLNKLYSHLPLKLKHSPELNLPSQHSPETLTSSQLKALVLSR 107

Query: 61  FSQGKFFDLFQNVVASPSVLLTASQNLISSPLSNAPDSLPVFELVSNCFSVEVMARELSE 120
           FS GKF DLFQNVVASPSVLLTASQNLI+ P SNAPDSLP+F+LVS CFSVEVMARELSE
Sbjct: 108 FSHGKFVDLFQNVVASPSVLLTASQNLITPPFSNAPDSLPLFDLVSKCFSVEVMARELSE 167

Query: 121 NRFDVGACCVRMAPLEEKGESLVLPNLKLKVLIEAIRIVLEIVYDERFVTFSYGGRVGMG 180
           NRFDVGACCV MAPLEEKGESL+LPNLKLKVLIEAIR+V+EIVYDERFVTFSYGGRVGMG
Sbjct: 168 NRFDVGACCVPMAPLEEKGESLLLPNLKLKVLIEAIRMVMEIVYDERFVTFSYGGRVGMG 227

Query: 181 RHTAIRYLKNSVQNPSWWFTVAFRHKKFESVHVNTLCLLMQEKIKDDTLIYMLRKLFELE 240
           RHTAIRYLKNSVQNPSWWFTVAFR KKFESVHVNTLCLLMQEKIKDD LIYMLRKLFE+E
Sbjct: 228 RHTAIRYLKNSVQNPSWWFTVAFRRKKFESVHVNTLCLLMQEKIKDDILIYMLRKLFEVE 287

Query: 241 AIQIELGAFYLGRGFPQESSLCSILFNIYFNGFDKEIQQIRLQKNEENPKFKLDEIVSFH 300
           AIQIELG  YLGRGFPQES LCSIL NIYFNGFDKEIQ+IRLQK EENPKF LDEIVSFH
Sbjct: 288 AIQIELGGCYLGRGFPQESGLCSILLNIYFNGFDKEIQRIRLQKIEENPKFNLDEIVSFH 347

Query: 301 NPVKVYAVRYLDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRMNTAIHSAVSEKIS 360
           NPVK+YAVRYLDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRMNTAIHSAVSEKI 
Sbjct: 348 NPVKIYAVRYLDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRMNTAIHSAVSEKIG 407

Query: 361 FLGMELQAVPPSVLHPPMSEKAVRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVF 420
           FLGMEL+AVPPSVLHPPMSEKA+RARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVF
Sbjct: 408 FLGMELRAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVF 467

Query: 421 KKLKQTSGFKSEFQIEKEVRDIFRNWADEVVQDFFESSEDHAEWHRLLSAGDFLSLKHIR 480
           KK K+T GFKSEFQIEKEVR IFRNWADEVVQDFFESSEDHAEWHR+LSAGDFLSLKHIR
Sbjct: 468 KKFKRTGGFKSEFQIEKEVRSIFRNWADEVVQDFFESSEDHAEWHRVLSAGDFLSLKHIR 527

Query: 481 NQLPENLVNAYDRFQDQVNKHLNPVKVKKEKAREDEEKRVEEEELYAKRTVEDLTRLCIK 540
           NQLPE+LVNAYDRFQDQVNKHLNPVK KKEKAREDEEKR+EEEELYAKRTV+DLTRLCIK
Sbjct: 528 NQLPEDLVNAYDRFQDQVNKHLNPVKFKKEKAREDEEKRLEEEELYAKRTVDDLTRLCIK 587

Query: 541 VDAPIELVRKAVKMVGFTNKMGRPRPISSLLALEDADIIKWYSGVGRRWLDFFCCCHNYK 600
           VDAPIELVRKAV+MVGFTN MGRPRPISSL+ LEDADIIKWYSGVGRRWLDFFCCCHNYK
Sbjct: 588 VDAPIELVRKAVRMVGFTNNMGRPRPISSLIVLEDADIIKWYSGVGRRWLDFFCCCHNYK 647

Query: 601 TVKTVVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVSDLNGNEEMHFPTEREVKMLG 660
            VKTVVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKV DLNGNEEMHFPTE+EVKMLG
Sbjct: 648 MVKTVVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGNEEMHFPTEKEVKMLG 707

Query: 661 ERNLVDPYPVDGALSLLLIRLATDEASYPCIAHFCNRTDSILYRVRLLQRTLNVNPSNGV 720
           ERNL DPYPVDGALSLLLIRLATDEASYPCIA+FCNRT+SILYRVRLLQRTLNVNPS+GV
Sbjct: 708 ERNLADPYPVDGALSLLLIRLATDEASYPCIANFCNRTNSILYRVRLLQRTLNVNPSDGV 767

Query: 721 EWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 768
           EWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD
Sbjct: 768 EWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 814

[Close]
BLAST of Lsi05G005990 vs. NCBI nr
Match: KAE8650134.1 (hypothetical protein Csa_009531 [Cucumis sativus])

HSP 1 Score: 1401.3 bits (3626), Expect = 0.0e+00
Identity = 710/767 (92.57%), Postives = 732/767 (95.44%), Query Frame = 0 

Query: 1   MFIQFRKIATPLKPKFSNSLTKPYFHLPLKLKHSSLLKLPSQHSPETLTRPQLKALVLSH 60
           MFI FRKIAT LKPKFSNSL K Y HLPLKLKHS  L LPSQHSPETLT  QLKALVLS 
Sbjct: 1   MFIHFRKIATSLKPKFSNSLNKLYSHLPLKLKHSPELNLPSQHSPETLTSSQLKALVLSR 60

Query: 61  FSQGKFFDLFQNVVASPSVLLTASQNLISSPLSNAPDSLPVFELVSNCFSVEVMARELSE 120
           FS GKF DLFQNVVASPSVLLTASQNLI+ P SNAPDSLP+F+LVS CFSVEVMARELSE
Sbjct: 61  FSHGKFVDLFQNVVASPSVLLTASQNLITPPFSNAPDSLPLFDLVSKCFSVEVMARELSE 120

Query: 121 NRFDVGACCVRMAPLEEKGESLVLPNLKLKVLIEAIRIVLEIVYDERFVTFSYGGRVGMG 180
           NRFDVGACCV MAPLEEKGESL+LPNLKLKVLIEAIR+V+EIVYDERFVTFSYGGRVGMG
Sbjct: 121 NRFDVGACCVPMAPLEEKGESLLLPNLKLKVLIEAIRMVMEIVYDERFVTFSYGGRVGMG 180

Query: 181 RHTAIRYLKNSVQNPSWWFTVAFRHKKFESVHVNTLCLLMQEKIKDDTLIYMLRKLFELE 240
           RHTAIRYLKNSVQNPSWWFTVAFR KKFESVHVNTLCLLMQEKIKDD LIYMLRKLFE+E
Sbjct: 181 RHTAIRYLKNSVQNPSWWFTVAFRRKKFESVHVNTLCLLMQEKIKDDILIYMLRKLFEVE 240

Query: 241 AIQIELGAFYLGRGFPQESSLCSILFNIYFNGFDKEIQQIRLQKNEENPKFKLDEIVSFH 300
           AIQIELG  YLGRGFPQES LCSIL NIYFNGFDKEIQ+IRLQK EENPKF LDEIVSFH
Sbjct: 241 AIQIELGGCYLGRGFPQESGLCSILLNIYFNGFDKEIQRIRLQKIEENPKFNLDEIVSFH 300

Query: 301 NPVKVYAVRYLDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRMNTAIHSAVSEKIS 360
           NPVK+YAVRYLDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRMNTAIHSAVSEKI 
Sbjct: 301 NPVKIYAVRYLDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRMNTAIHSAVSEKIG 360

Query: 361 FLGMELQAVPPSVLHPPMSEKAVRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVF 420
           FLGMEL+AVPPSVLHPPMSEKA+RARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVF
Sbjct: 361 FLGMELRAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVF 420

Query: 421 KKLKQTSGFKSEFQIEKEVRDIFRNWADEVVQDFFESSEDHAEWHRLLSAGDFLSLKHIR 480
           KK K+T GFKSEFQIEKEVR IFRNWADEVVQDFFESSEDHAEWHR+LSAGDFLSLKHIR
Sbjct: 421 KKFKRTGGFKSEFQIEKEVRSIFRNWADEVVQDFFESSEDHAEWHRVLSAGDFLSLKHIR 480

Query: 481 NQLPENLVNAYDRFQDQVNKHLNPVKVKKEKAREDEEKRVEEEELYAKRTVEDLTRLCIK 540
           NQLPE+LVNAYDRFQDQVNKHLNPVK KKEKAREDEEKR+EEEELYAKRTV+DLTRLCIK
Sbjct: 481 NQLPEDLVNAYDRFQDQVNKHLNPVKFKKEKAREDEEKRLEEEELYAKRTVDDLTRLCIK 540

Query: 541 VDAPIELVRKAVKMVGFTNKMGRPRPISSLLALEDADIIKWYSGVGRRWLDFFCCCHNYK 600
           VDAPIELVRKAV+MVGFTN MGRPRPISSL+ LEDADIIKWYSGVGRRWLDFFCCCHNYK
Sbjct: 541 VDAPIELVRKAVRMVGFTNNMGRPRPISSLIVLEDADIIKWYSGVGRRWLDFFCCCHNYK 600

Query: 601 TVKTVVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVSDLNGNEEMHFPTEREVKMLG 660
            VKTVVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKV DLNGNEEMHFPTE+EVKMLG
Sbjct: 601 MVKTVVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGNEEMHFPTEKEVKMLG 660

Query: 661 ERNLVDPYPVDGALSLLLIRLATDEASYPCIAHFCNRTDSILYRVRLLQRTLNVNPSNGV 720
           ERNL DPYPVDGALSLLLIRLATDEASYPCIA+FCNRT+SILYRVRLLQRTLNVNPS+GV
Sbjct: 661 ERNLADPYPVDGALSLLLIRLATDEASYPCIANFCNRTNSILYRVRLLQRTLNVNPSDGV 720

Query: 721 EWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 768
           EWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD
Sbjct: 721 EWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 767

[Close]
BLAST of Lsi05G005990 vs. NCBI nr
Match: XP_008463418.1 (PREDICTED: uncharacterized protein LOC103501588 [Cucumis melo] >XP_016903029.1 PREDICTED: uncharacterized protein LOC103501588 [Cucumis melo] >XP_016903030.1 PREDICTED: uncharacterized protein LOC103501588 [Cucumis melo] >XP_016903031.1 PREDICTED: uncharacterized protein LOC103501588 [Cucumis melo] >XP_016903032.1 PREDICTED: uncharacterized protein LOC103501588 [Cucumis melo] >XP_016903033.1 PREDICTED: uncharacterized protein LOC103501588 [Cucumis melo] >XP_016903034.1 PREDICTED: uncharacterized protein LOC103501588 [Cucumis melo])

HSP 1 Score: 1382.1 bits (3576), Expect = 0.0e+00
Identity = 704/767 (91.79%), Postives = 728/767 (94.92%), Query Frame = 0 

Query: 1   MFIQFRKIATPLKPKFSNSLTKPYFHLPLKLKHSSLLKLPSQHSPETLTRPQLKALVLSH 60
           MFI FRKIAT LKPKFSNSL K Y HLPL+ K   +L LPSQHSPE+LT  QLKALVLS 
Sbjct: 1   MFIHFRKIATSLKPKFSNSLNKLYSHLPLRKK---VLNLPSQHSPESLTSSQLKALVLSR 60

Query: 61  FSQGKFFDLFQNVVASPSVLLTASQNLISSPLSNAPDSLPVFELVSNCFSVEVMARELSE 120
           FS GKF DLFQNVVASPSVLLTAS+NLI+ P SNAPDSLP+ +LVS CFSVEVMARELSE
Sbjct: 61  FSHGKFVDLFQNVVASPSVLLTASRNLITPPFSNAPDSLPLSDLVSKCFSVEVMARELSE 120

Query: 121 NRFDVGACCVRMAPLEEKGESLVLPNLKLKVLIEAIRIVLEIVYDERFVTFSYGGRVGMG 180
           NRFDVGACCV MAPLEEKGESLVLPNLKLKVLIEAIR+VLEIVYDERFVTFSYGGRVGMG
Sbjct: 121 NRFDVGACCVPMAPLEEKGESLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGMG 180

Query: 181 RHTAIRYLKNSVQNPSWWFTVAFRHKKFESVHVNTLCLLMQEKIKDDTLIYMLRKLFELE 240
           RHTAIRYLKNSVQNPSWWFTVAFR KKFESVHVNTLCLLMQEKIKDD LIYMLRKLFE+E
Sbjct: 181 RHTAIRYLKNSVQNPSWWFTVAFRRKKFESVHVNTLCLLMQEKIKDDILIYMLRKLFEVE 240

Query: 241 AIQIELGAFYLGRGFPQESSLCSILFNIYFNGFDKEIQQIRLQKNEENPKFKLDEIVSFH 300
           AIQIELG  YLGRGFPQES LCSIL NIYFNGFDKEIQ+IRLQKNEENPKF LDEIVSFH
Sbjct: 241 AIQIELGGCYLGRGFPQESGLCSILLNIYFNGFDKEIQRIRLQKNEENPKFNLDEIVSFH 300

Query: 301 NPVKVYAVRYLDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRMNTAIHSAVSEKIS 360
           NPVK+YAVRYLDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRMNTAIHSAVSEKI 
Sbjct: 301 NPVKIYAVRYLDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRMNTAIHSAVSEKIG 360

Query: 361 FLGMELQAVPPSVLHPPMSEKAVRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVF 420
           FLGMELQAV PSVLHPPMSEKA+RARKKYLRQKEVRAIELRNARERNRKKLGLKILSH+F
Sbjct: 361 FLGMELQAVTPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHMF 420

Query: 421 KKLKQTSGFKSEFQIEKEVRDIFRNWADEVVQDFFESSEDHAEWHRLLSAGDFLSLKHIR 480
           KK K+T GFKSEFQIE EVR IFRNWADEVVQDFFESSEDHAEWHR+LSAGDFLSLKHIR
Sbjct: 421 KKFKRTGGFKSEFQIETEVRSIFRNWADEVVQDFFESSEDHAEWHRVLSAGDFLSLKHIR 480

Query: 481 NQLPENLVNAYDRFQDQVNKHLNPVKVKKEKAREDEEKRVEEEELYAKRTVEDLTRLCIK 540
           NQLPE+LVNAYDRFQ QVNKHLNPVKVKKEKAREDEEKR+EEEELYAKRTVEDLTRLCIK
Sbjct: 481 NQLPEDLVNAYDRFQYQVNKHLNPVKVKKEKAREDEEKRLEEEELYAKRTVEDLTRLCIK 540

Query: 541 VDAPIELVRKAVKMVGFTNKMGRPRPISSLLALEDADIIKWYSGVGRRWLDFFCCCHNYK 600
           VDAPIELVRKAV+MVGFTNKMGRPRPISSL+ALEDADIIKWYSGVGRRWLDFFCCCHNYK
Sbjct: 541 VDAPIELVRKAVRMVGFTNKMGRPRPISSLIALEDADIIKWYSGVGRRWLDFFCCCHNYK 600

Query: 601 TVKTVVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVSDLNGNEEMHFPTEREVKMLG 660
            VKTVVTYHLRFSCILTLAEKHESTKREAMKHY KDLKV DLNGNEEMHFPTE+ VKMLG
Sbjct: 601 MVKTVVTYHLRFSCILTLAEKHESTKREAMKHYGKDLKVFDLNGNEEMHFPTEKAVKMLG 660

Query: 661 ERNLVDPYPVDGALSLLLIRLATDEASYPCIAHFCNRTDSILYRVRLLQRTLNVNPSNGV 720
           ERNL DPYPVDGALSLLLIRLATDEASYPCIA+FCNRT+SILYRVRLLQRTLNVNPS+GV
Sbjct: 661 ERNLADPYPVDGALSLLLIRLATDEASYPCIANFCNRTNSILYRVRLLQRTLNVNPSDGV 720

Query: 721 EWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 768
           EWV+GMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD
Sbjct: 721 EWVKGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 764

[Close]
BLAST of Lsi05G005990 vs. NCBI nr
Match: KAA0033971.1 (RNA-directed DNA polymerase [Cucumis melo var. makuwa])

HSP 1 Score: 1379.4 bits (3569), Expect = 0.0e+00
Identity = 704/767 (91.79%), Postives = 727/767 (94.78%), Query Frame = 0 

Query: 1   MFIQFRKIATPLKPKFSNSLTKPYFHLPLKLKHSSLLKLPSQHSPETLTRPQLKALVLSH 60
           MFI FRKIAT LKPKFSNSL K Y HLPL+ K   +L LPSQHSPE+LT  QLKALVLS 
Sbjct: 1   MFIHFRKIATSLKPKFSNSLNKLYSHLPLRKK---VLNLPSQHSPESLTSSQLKALVLSR 60

Query: 61  FSQGKFFDLFQNVVASPSVLLTASQNLISSPLSNAPDSLPVFELVSNCFSVEVMARELSE 120
           FS GKF DLFQNVVASPSVLLTAS+NLI+ P SNAPDSLP+ +LVS CFSVEVMARELSE
Sbjct: 61  FSHGKFVDLFQNVVASPSVLLTASRNLITPPFSNAPDSLPLSDLVSKCFSVEVMARELSE 120

Query: 121 NRFDVGACCVRMAPLEEKGESLVLPNLKLKVLIEAIRIVLEIVYDERFVTFSYGGRVGMG 180
           NRFDVGACCV MAPLEEKGESLVLPNLKLKVLIEAIR+VLEIVYDERFVTFSYGGRVGMG
Sbjct: 121 NRFDVGACCVPMAPLEEKGESLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGMG 180

Query: 181 RHTAIRYLKNSVQNPSWWFTVAFRHKKFESVHVNTLCLLMQEKIKDDTLIYMLRKLFELE 240
           RHTAIRYLKNSVQNPSWWFTVAFR KKFESVHVNTLCLLMQEKIKDD LIYMLRKLFE+E
Sbjct: 181 RHTAIRYLKNSVQNPSWWFTVAFRRKKFESVHVNTLCLLMQEKIKDDILIYMLRKLFEVE 240

Query: 241 AIQIELGAFYLGRGFPQESSLCSILFNIYFNGFDKEIQQIRLQKNEENPKFKLDEIVSFH 300
           AIQIELG  YLGRGFPQES LCSIL NIYFNGFDKEIQ+IRLQKNEENPKF LDEIVSFH
Sbjct: 241 AIQIELGGCYLGRGFPQESGLCSILLNIYFNGFDKEIQRIRLQKNEENPKFNLDEIVSFH 300

Query: 301 NPVKVYAVRYLDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRMNTAIHSAVSEKIS 360
           NPVK+YAVRYLDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRMNTAIHSAVSEKI 
Sbjct: 301 NPVKIYAVRYLDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRMNTAIHSAVSEKIG 360

Query: 361 FLGMELQAVPPSVLHPPMSEKAVRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVF 420
           FLGMELQAV PSVLHPPMSEKA+RARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVF
Sbjct: 361 FLGMELQAVTPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVF 420

Query: 421 KKLKQTSGFKSEFQIEKEVRDIFRNWADEVVQDFFESSEDHAEWHRLLSAGDFLSLKHIR 480
           KK K+T GFKSEFQIE EVR IFRNWADEVVQDFFESSED AEWHR+LSAGDFLSLKHIR
Sbjct: 421 KKFKRTGGFKSEFQIETEVRSIFRNWADEVVQDFFESSEDPAEWHRVLSAGDFLSLKHIR 480

Query: 481 NQLPENLVNAYDRFQDQVNKHLNPVKVKKEKAREDEEKRVEEEELYAKRTVEDLTRLCIK 540
           NQLPE+LVNAYDRFQ QVNKHLNPVKVKKEKAREDEEKR+EEEELYAKRTVEDLTRLCIK
Sbjct: 481 NQLPEDLVNAYDRFQYQVNKHLNPVKVKKEKAREDEEKRLEEEELYAKRTVEDLTRLCIK 540

Query: 541 VDAPIELVRKAVKMVGFTNKMGRPRPISSLLALEDADIIKWYSGVGRRWLDFFCCCHNYK 600
           VDAPIELVRKAV+MVGFTNKMGRPRPISSL+ALEDADIIKWYSGVGRRWLDFFCCCHNYK
Sbjct: 541 VDAPIELVRKAVRMVGFTNKMGRPRPISSLIALEDADIIKWYSGVGRRWLDFFCCCHNYK 600

Query: 601 TVKTVVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVSDLNGNEEMHFPTEREVKMLG 660
            VKTVVTYHLRFSCILTLAEKHESTKREAMKHY KDLKV DLNGNEEMHFPTE+ VKMLG
Sbjct: 601 MVKTVVTYHLRFSCILTLAEKHESTKREAMKHYGKDLKVFDLNGNEEMHFPTEKAVKMLG 660

Query: 661 ERNLVDPYPVDGALSLLLIRLATDEASYPCIAHFCNRTDSILYRVRLLQRTLNVNPSNGV 720
           ERNL DPYPVDGALSLLLIRLATDEASYPCIA+FCNRT+SILYRVRLLQRTLNVNPS+GV
Sbjct: 661 ERNLADPYPVDGALSLLLIRLATDEASYPCIANFCNRTNSILYRVRLLQRTLNVNPSDGV 720

Query: 721 EWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 768
           EWV+GMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD
Sbjct: 721 EWVKGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 764

[Close]
BLAST of Lsi05G005990 vs. TAIR 10
Match: AT5G04050.2 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 718.8 bits (1854), Expect = 4.7e-207
Identity = 403/739 (54.53%), Postives = 495/739 (66.98%), Query Frame = 0 

Query: 34  SSLLKLPSQHSPETLTRPQLKALVLSHFSQGKFFDLFQNVVASPSVLLTASQNLISSPLS 93
           SSL     Q   E L + +L+ALVL  +S GKF+ L +N V+ P VLL A QNL  S   
Sbjct: 30  SSLFLNSDQTITEPLVKSELEALVLKQYSHGKFYSLVKNAVSLPCVLLAACQNLSLS--- 89

Query: 94  NAPDSLPVFELVSNCFSVEVMARELSENRFDVGACCVRMAPLEEKGESLVLPNLKLKVLI 153
            A  S  + + VS  FS+E M RE+ E RFD+ +CCV     E    SLVLPNLKLKVLI
Sbjct: 90  -ANSSGDLADRVSRRFSIEEMGREIREGRFDIRSCCV-----EFISSSLVLPNLKLKVLI 149

Query: 154 EAIRIVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKNSVQNPSWWFTVAFRHKKFESVHV 213
           EAIR+VLEIVYD+RF TFSYGGRVGMGRHTAIRYLKNSV+NP WWF V+F  + FE  +V
Sbjct: 150 EAIRMVLEIVYDDRFATFSYGGRVGMGRHTAIRYLKNSVENPRWWFRVSFAREMFEERNV 209

Query: 214 NTLCLLMQEKIKDDTLIYMLRKLFELEAIQIELGAFYLGRGFPQESSLCSILFNIYFNGF 273
           + LC  + EKI D  LI M++KLFE   ++IELG    GRGFPQE  LCSIL N+YF+G 
Sbjct: 210 DILCGFVGEKINDVMLIEMIKKLFEFGILKIELGGCNSGRGFPQECGLCSILINVYFDGL 269

Query: 274 DKEIQQIRLQKNEENPKFKLDEIVS----FHNPVKVYAVRYLDEILVITSGSKMLTMELK 333
           DKEIQ +RL+   +NP+    +  S    F  PV +YAVRYLDEILVITSGSKMLTM+LK
Sbjct: 270 DKEIQDLRLKMKVKNPRVGTGDEESTGNVFFKPVNIYAVRYLDEILVITSGSKMLTMDLK 329

Query: 334 SQVLKYLEGNLELEVDRMNTAIHSAVSEKISFLGMELQAVPPSVLHPPMSEKAVRARKKY 393
            +++  LE  LEL VDR+NT+IHSAVSEKI+FLGM LQAVPPSVL PP SEKAVRA KKY
Sbjct: 330 KRIVDILEQRLELRVDRLNTSIHSAVSEKINFLGMYLQAVPPSVLRPPKSEKAVRAMKKY 389

Query: 394 LRQKEVRAIELRNARERNRKKLGLKILSHVFKKLKQTSGFKSEFQIEKEVRDIFRNWADE 453
            RQK+VR +ELRNARERNRK LGLKI  HV KK+KQ++GFK E +IE EVRDIF++W +E
Sbjct: 390 QRQKDVRKLELRNARERNRKTLGLKIFRHVLKKIKQSNGFKFEGEIENEVRDIFQSWGEE 449

Query: 454 VVQDFFESSEDHAEWHRLLSAGDFLSLKHIRNQLPENLVNAYDRFQDQVNKHLNPVKVKK 513
           V+QDF  S E+  +WH LL+ GDFLSL+HIR +LP++L++AYD FQ+QV+KHL P + K 
Sbjct: 450 VMQDFMGSLEERWKWHWLLTRGDFLSLRHIREKLPQDLIDAYDEFQEQVDKHLAPTQAK- 509

Query: 514 EKAREDEEKRVEEEE--LYAKRTVEDLTRLCIKVDAPIELVRKAVKMVGFTNKMGRPRPI 573
            K  EDEE+RVEEEE   YA+RTVEDLT+LC+KV AP ELVRKA+               
Sbjct: 510 -KVLEDEERRVEEEEEQRYAERTVEDLTKLCMKVSAPEELVRKAI--------------- 569

Query: 574 SSLLALEDADIIKWYSGVGRRWLDFFCCCHNYKTVKTVVTYHLRFSCILTLAEKHESTKR 633
                                                                       
Sbjct: 570 ------------------------------------------------------------ 629

Query: 634 EAMKHYSKDLKVSDLNGNEEMHFPTEREVKMLGERNLVDPYPVDGALSLLLIRLATDEAS 693
                     KVSDL+G EE HFP+EREVKM+G++NL DP PVDG LSLLLIRLA+DE  
Sbjct: 630 ----------KVSDLDGREEAHFPSEREVKMMGDKNLSDPKPVDGTLSLLLIRLASDEPL 672

Query: 694 YPCIAHFCNRTDSILYRVRLLQRTLNVNPSNGVEWVRGMGVIHESLNQRCLPLCADHISD 753
           + C A FC R+D+I++RV LLQ  L++NP +  +WV GMG IH +LN++CLPLC+ HISD
Sbjct: 690 HHCAASFCERSDTIMHRVHLLQNRLHINPLDEEKWVPGMGTIHSALNRKCLPLCSTHISD 672

Query: 754 LYMGKINLQDLDCTLSLDM 767
           +Y+GKI LQD+D +  +D+
Sbjct: 750 VYLGKITLQDVDSSSFIDL 672

[Close]
BLAST of Lsi05G005990 vs. TAIR 10
Match: AT5G04050.1 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 683.7 bits (1763), Expect = 1.7e-196
Identity = 366/579 (63.21%), Postives = 440/579 (75.99%), Query Frame = 0 

Query: 34  SSLLKLPSQHSPETLTRPQLKALVLSHFSQGKFFDLFQNVVASPSVLLTASQNLISSPLS 93
           SSL     Q   E L + +L+ALVL  +S GKF+ L +N V+ P VLL A QNL  S   
Sbjct: 30  SSLFLNSDQTITEPLVKSELEALVLKQYSHGKFYSLVKNAVSLPCVLLAACQNLSLS--- 89

Query: 94  NAPDSLPVFELVSNCFSVEVMARELSENRFDVGACCVRMAPLEEKGESLVLPNLKLKVLI 153
            A  S  + + VS  FS+E M RE+ E RFD+ +CCV     E    SLVLPNLKLKVLI
Sbjct: 90  -ANSSGDLADRVSRRFSIEEMGREIREGRFDIRSCCV-----EFISSSLVLPNLKLKVLI 149

Query: 154 EAIRIVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKNSVQNPSWWFTVAFRHKKFESVHV 213
           EAIR+VLEIVYD+RF TFSYGGRVGMGRHTAIRYLKNSV+NP WWF V+F  + FE  +V
Sbjct: 150 EAIRMVLEIVYDDRFATFSYGGRVGMGRHTAIRYLKNSVENPRWWFRVSFAREMFEERNV 209

Query: 214 NTLCLLMQEKIKDDTLIYMLRKLFELEAIQIELGAFYLGRGFPQESSLCSILFNIYFNGF 273
           + LC  + EKI D  LI M++KLFE   ++IELG    GRGFPQE  LCSIL N+YF+G 
Sbjct: 210 DILCGFVGEKINDVMLIEMIKKLFEFGILKIELGGCNSGRGFPQECGLCSILINVYFDGL 269

Query: 274 DKEIQQIRLQKNEENPKFKLDEIVS----FHNPVKVYAVRYLDEILVITSGSKMLTMELK 333
           DKEIQ +RL+   +NP+    +  S    F  PV +YAVRYLDEILVITSGSKMLTM+LK
Sbjct: 270 DKEIQDLRLKMKVKNPRVGTGDEESTGNVFFKPVNIYAVRYLDEILVITSGSKMLTMDLK 329

Query: 334 SQVLKYLEGNLELEVDRMNTAIHSAVSEKISFLGMELQAVPPSVLHPPMSEKAVRARKKY 393
            +++  LE  LEL VDR+NT+IHSAVSEKI+FLGM LQAVPPSVL PP SEKAVRA KKY
Sbjct: 330 KRIVDILEQRLELRVDRLNTSIHSAVSEKINFLGMYLQAVPPSVLRPPKSEKAVRAMKKY 389

Query: 394 LRQKEVRAIELRNARERNRKKLGLKILSHVFKKLKQTSGFKSEFQIEKEVRDIFRNWADE 453
            RQK+VR +ELRNARERNRK LGLKI  HV KK+KQ++GFK E +IE EVRDIF++W +E
Sbjct: 390 QRQKDVRKLELRNARERNRKTLGLKIFRHVLKKIKQSNGFKFEGEIENEVRDIFQSWGEE 449

Query: 454 VVQDFFESSEDHAEWHRLLSAGDFLSLKHIRNQLPENLVNAYDRFQDQVNKHLNPVKVKK 513
           V+QDF  S E+  +WH LL+ GDFLSL+HIR +LP++L++AYD FQ+QV+KHL P + K 
Sbjct: 450 VMQDFMGSLEERWKWHWLLTRGDFLSLRHIREKLPQDLIDAYDEFQEQVDKHLAPTQAK- 509

Query: 514 EKAREDEEKRVEEEE--LYAKRTVEDLTRLCIKVDAPIELVRKAVKMVGFTNKMGRPRPI 573
            K  EDEE+RVEEEE   YA+RTVEDLT+LC+KV AP ELVRKA+K+VGFTN MGRPRPI
Sbjct: 510 -KVLEDEERRVEEEEEQRYAERTVEDLTKLCMKVSAPEELVRKAIKLVGFTNSMGRPRPI 569

Query: 574 SSLLALEDADIIKWYSGVGRRWLDFFCCCHNYKTVKTVV 607
             L+ LED+DIIKWY+GVGR+WLDFFCCCHNYK VK++V
Sbjct: 570 IHLVTLEDSDIIKWYAGVGRKWLDFFCCCHNYKMVKSLV 597

[Close]
BLAST of Lsi05G005990 vs. TAIR 10
Match: AT1G74350.1 (Intron maturase, type II family protein )

HSP 1 Score: 226.9 bits (577), Expect = 5.6e-59
Identity = 203/753 (26.96%), Postives = 338/753 (44.89%), Query Frame = 0 

Query: 52  QLKALVLSHFSQGKFFDLFQNVVASPSVLLTASQNL-ISSPLSNAPDSLPVFELVSNCFS 111
           +LK  V      GKF DL + V+A P  L  A   + ++S +S    +  V        +
Sbjct: 45  RLKKRVKEQCINGKFSDLLKKVIARPETLRDAYDCIRLNSNVSITERNGSV--------A 104

Query: 112 VEVMARELSENRFDVGACCVRMAPLEEKGESLVLPNLKLKVLIEAIRIVLEIVYDERFVT 171
            + +A ELS   FDV +    +   ++  E LVLP++ LKV+ EAIRIVLE+V+   F  
Sbjct: 105 FDSIAEELSSGVFDVASNTFSIVARDKTKEVLVLPSVALKVVQEAIRIVLEVVFSPHFSK 164

Query: 172 FSYGGRVGMGRHTAIRYLKNSVQNPSWWFTVAFRHKKFESVHVNTLCLLMQEKIKDDTLI 231
            S+  R G GR +A++Y+ N++    W FT++   K   SV  N L  +M+EK++D +L 
Sbjct: 165 ISHSCRSGRGRASALKYINNNISRSDWCFTLSLNKKLDVSVFENLLS-VMEEKVEDSSLS 224

Query: 232 YMLRKLFELEAIQIELGAFYLGRGFPQESSLCSILFNIYFNGFDKEIQQIRL-------- 291
            +LR +FE   + +E G F  G G PQE  L  +L NIY + FD E  +I +        
Sbjct: 225 ILLRSMFEARVLNLEFGGFPKGHGLPQEGVLSRVLMNIYLDRFDHEFYRISMRHEALGLD 284

Query: 292 -QKNEENPKFK-------------LDEIVSFHNPVKVYAVRYLDEILVITSGSKMLTMEL 351
            + +E++P  K             L         ++VY  R++DEI    SG K +  ++
Sbjct: 285 SKTDEDSPGSKLRSWFRRQAGEQGLKSTTEQDVALRVYCCRFMDEIYFSVSGPKKVASDI 344

Query: 352 KSQVLKYLEGNLELEVDRMNTAIHSAVSEKISFLGMELQAVPPSVLHPPMSEKAVRARKK 411
           +S+ + +L  +L L++           +  +  LG     V  +V   P + KAV   K+
Sbjct: 345 RSEAIGFLRNSLHLDITDETDPSPCEATSGLRVLG---TLVRKNVRESP-TVKAVHKLKE 404

Query: 412 YLRQKEVRAIELRNARERNRKKLGLKILSHVFKKLKQTS----------------GFKSE 471
            +R   + A++   A      ++G K L H  KK+K++                   K+ 
Sbjct: 405 KVR---LFALQKEEAWTLGTVRIGKKWLGHGLKKVKESEIKGLADSNSTLSQISCHRKAG 464

Query: 472 FQIEKEVRDIFRNWADEVVQDFFESSEDHAEWHRLLSAGDFLSLKH-IRNQLPENLVNAY 531
            + +   + + R W ++V++    +S D +E        +F+  KH +   +P+ L +A+
Sbjct: 465 METDHWYKILLRIWMEDVLR----TSADRSE--------EFVLSKHVVEPTVPQELRDAF 524

Query: 532 DRFQDQVNKHLNPVKVKKEKAREDEEKRVEEEELYAKRTVEDLTRLCIKVDAPIELVRKA 591
            +FQ+    +++              +    E L       D       V AP   + + 
Sbjct: 525 YKFQNAAAAYVS-------------SETANLEALLPCPQSHDRPVFFGDVVAPTNAIGRR 584

Query: 592 VKMVGFTNKMGRPRPISSLLALEDADIIKWYSGVGRRWLDFFCCCHNYKTVKTVVTYHLR 651
           +   G     G  R  S L+ L+ A II WYSG+ RRW+ ++  C N+  +K ++   +R
Sbjct: 585 LYRYGLITAKGYARSNSMLILLDTAQIIDWYSGLVRRWVIWYEGCSNFDEIKALIDNQIR 644

Query: 652 FSCILTLAEKHESTKREAMKHYSKDLKVSDLNGNEEMHFPTERE----VKMLGERNLVDP 711
            SCI TLA K+   + E  K    DL++S +   E++    + E         + +L   
Sbjct: 645 MSCIRTLAAKYRIHENEIEKRL--DLELSTIPSAEDIEQEIQHEKLDSPAFDRDEHLTYG 704

Query: 712 YPVDGALSLLLIRLATDEASYPCIAHFCNRTDSILYRVRLLQRTLNVNPSNGVEWVRGMG 760
               G   L L RL ++     C    C+     +Y +  ++R           W  G  
Sbjct: 705 LSNSGLCLLSLARLVSESRPCNCFVIGCSMAAPAVYTLHAMER------QKFPGWKTGFS 748

[Close]
BLAST of Lsi05G005990 vs. TAIR 10
Match: ATMG00520.1 (Intron maturase, type II family protein )

HSP 1 Score: 68.6 bits (166), Expect = 2.6e-11
Identity = 88/375 (23.47%), Postives = 162/375 (43.20%), Query Frame = 0 

Query: 300 HNPVKVYAVRYLDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRMNTAIHSAVSEKI 359
           H  +++   RY D++L+   G+  L +E++ ++  +L+  L L V    +   +A S  +
Sbjct: 311 HYLIRICYARYADDLLLGIVGAVELLIEIQKRIAHFLQSGLNLWVGSAGSTTIAARS-TV 370

Query: 360 SFLGMELQAVPPSVLHPPMSEKAVRARKKYLRQKEVRAIELRNARERNRKKLGLKI-LSH 419
            FLG  ++ VPP    P    + +  R +   +  + A  LR+A     + LG  I +  
Sbjct: 371 EFLGTVIREVPPRTT-PIQFLRELEKRLRVKHRIHITACHLRSAIHSKFRNLGDSIPIKQ 430

Query: 420 VFKKLKQTSGFKSEFQIEKEVRDIFRNWADEVVQDFFESSEDHAEWHRLLSAGDFLSLKH 479
           + K + +T   +   Q+           A+ +      S +    W          ++KH
Sbjct: 431 LTKGMSKTGSLQDGVQL-----------AETLGTAGVRSPQVSVLWG---------TVKH 490

Query: 480 IRNQL-------PENLVNAYDRFQDQVNK---HLNPVKVKKEKAREDEEKRVEEEELYAK 539
           IR               NA    Q  V++   H   + +     R    K   E   +  
Sbjct: 491 IRQGSRGISFLHSSGRSNASSDVQQVVSRSGTHARKLSLYTPPGR----KAAGEGGGHWA 550

Query: 540 RTVEDLTRLCIKVDAPIELVRKAVKMVGFTNKMGRPRPI--SSLLALEDADIIKWYSGVG 599
            ++   +   IK++API+ + + ++  G  ++  RP PI  + L  + D DI+ W +G+ 
Sbjct: 551 GSIS--SEFPIKIEAPIKKILRRLRDRGIISRR-RPWPIHVACLTNVSDEDIVNWSAGIA 610

Query: 600 RRWLDFFCCCHNYKTVKTVVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVSDLNGNE 659
              L ++ C  N   V+T+V + +R+S I TLA KH+S+    +  YSKD  + +  G +
Sbjct: 611 ISPLSYYRCRDNLYQVRTIVDHQIRWSAIFTLAHKHKSSAPNIILKYSKDSNIVNQEGGK 656

Query: 660 EM-HFPTEREVKMLG 661
            +  FP   E+  LG
Sbjct: 671 ILAEFPNSIELGKLG 656

The following BLAST results are available for this feature:
BLAST of Lsi05G005990 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9LZA51.9e-23259.00Nuclear intron maturase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NMAT... [more]
Q9CA787.9e-5826.96Nuclear intron maturase 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NMAT... [more]
B1N1A31.7e-1226.52Putative nicotine oxidoreductase OS=Pseudomonas putida OX=303 GN=nicA PE=4 SV=1[more]
P038761.5e-1125.19Putative COX1/OXI3 intron 2 protein OS=Saccharomyces cerevisiae (strain ATCC 204... [more]
P0A3U05.6e-1126.18Group II intron-encoded protein LtrA OS=Lactococcus lactis subsp. cremoris OX=13... [more]
BLAST of Lsi05G005990 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A1S4E4Y10.0e+0091.79uncharacterized protein LOC103501588 OS=Cucumis melo OX=3656 GN=LOC103501588 PE=... [more]
A0A5A7SWY50.0e+0091.79RNA-directed DNA polymerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf... [more]
A0A6J1ILC20.0e+0087.01nuclear intron maturase 3, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC11147... [more]
A0A6J1FV640.0e+0087.14nuclear intron maturase 3, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1DNH60.0e+0085.49nuclear intron maturase 3, mitochondrial OS=Momordica charantia OX=3673 GN=LOC11... [more]
BLAST of Lsi05G005990 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038904122.10.0e+0094.00nuclear intron maturase 3, mitochondrial [Benincasa hispida][more]
XP_031738720.10.0e+0092.57nuclear intron maturase 3, mitochondrial [Cucumis sativus][more]
KAE8650134.10.0e+0092.57hypothetical protein Csa_009531 [Cucumis sativus][more]
XP_008463418.10.0e+0091.79PREDICTED: uncharacterized protein LOC103501588 [Cucumis melo] >XP_016903029.1 P... [more]
KAA0033971.10.0e+0091.79RNA-directed DNA polymerase [Cucumis melo var. makuwa][more]
BLAST of Lsi05G005990 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G04050.24.7e-20754.53RNA-directed DNA polymerase (reverse transcriptase) [more]
AT5G04050.11.7e-19663.21RNA-directed DNA polymerase (reverse transcriptase) [more]
AT1G74350.15.6e-5926.96Intron maturase, type II family protein [more]
ATMG00520.12.6e-1123.47Intron maturase, type II family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000477Reverse transcriptase domainPFAMPF00078RVT_1coord: 144..365
e-value: 7.0E-10
score: 38.8
IPR000477Reverse transcriptase domainPROSITEPS50878RT_POLcoord: 114..366
score: 8.670628
IPR024937Domain XPFAMPF01348Intron_maturas2coord: 541..647
e-value: 6.5E-16
score: 58.6
NoneNo IPR availablePANTHERPTHR33642:SF4NUCLEAR INTRON MATURASE 3, MITOCHONDRIALcoord: 32..763
NoneNo IPR availablePANTHERPTHR33642COX1/OXI3 INTRON 1 PROTEIN-RELATEDcoord: 32..763
NoneNo IPR availableCDDcd01651RT_G2_introncoord: 141..366
e-value: 2.02078E-38
score: 140.799

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G005990.1Lsi05G005990.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006315 homing of group II introns
biological_process GO:0090615 mitochondrial mRNA processing
biological_process GO:0006278 RNA-dependent DNA biosynthetic process
biological_process GO:0006397 mRNA processing
cellular_component GO:0005739 mitochondrion
molecular_function GO:0003964 RNA-directed DNA polymerase activity