Lsi04G010430 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G010430
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionAmino acid transporter
Locationchr04: 12252980 .. 12257595 (-)
RNA-Seq ExpressionLsi04G010430
SyntenyLsi04G010430
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCACCACCAACCACCATACGCCGCGTAACAGAAGATTTAACGACATTAATCTTCTCCTTCTCTTCATCTTCTTCTTCAGCTTTATTCTCTCCAGCTTATCATCGTATTGCCGCCGAGATGTCGCCGGCGCCGGAAAACGTACCTCTACTGCCGGGTGCGAAGCCGTGCACGAGGACTGCGTCGGTCTCCGGCGCCGTGTTCAACGTCTCGACCAGTATAATTGGCGCTGGAATCATGTCGATTCCCTTTGCTCTAAAGGTTCTCGGCATAATCCCCGCCCTCGTCTTGATCGTGTTCGTCGCTTTCCTTACTGACATATCGGTGGAGCTTTTGCTTAGATTCACTCATTCCGGCGACTCGACGACCTACGCCGGCGTCATGAAGGAGTCGTTCGGCCCGATTGGTTCGATTGCTACGCAAGTTTGTATCATGATTACTAATCTCGGTTGCTTGATTATGTACCAGATTATAATAGGTAAGTTTCTTTCGGACTTTAGAGATCTCAATCGTCTCTGCTTGATTTTACTTGCTTTGTAGTTTAATAGGCTGAAATTAGGGCTGGAATTTGGAAATTCTTCATATCTGATTGCCGTAACGGCCATAGATTTTAGGGAAACGGTTCGTGAAATCGCAAATTTTTTTCGATTCTCCAATTTTCCGATTGATTCTCGTAATCGTTCTTCTAAAATTGATGTGTGGAATTTACAGGGGATGTGTTCTCGGGCAATAAAGAAGGGGGAAAAGTGCATTTAGGAGTTTTACAAGAATGGTTTGGAAATCACTGGTGGAACACCAGAGAGTTCTCGATCCTTTTCACTCTCGTCTTCATTCTACTTCCTCTGGTTCTTCTCCGACGAGTTGGTCAGTAACTGGAAACTATGGAATCAATTAAACTGATGAGTGTATGATTTTTTAATTTTTTCGTTTTAATGGCGTCGGATTTGGATTTCTGCTATGTGTTTTGTTTTACAGATTCATTGAGATTTAGTTCGTTTGTATCGGTTGTTCTTGCCGTCGTTTTCGTTGGGATAAGCTCTGTATTGGCGATCATGGCGATCGTTGAAGGCAAAACGAAGAGCACCAGATTGGTACCGGAGCTTGACGAAGAAACCTCCTTCCTCGACCTTTTCACAGCTGTTCCAGTTCTTGTCACAGCTTTCTGCTTCCATTTCAATGGTAAACAAGAACGTACAAAACCTTATTCTTTGAAACAGAGTACTTCTTCGGTTTGGTTCGGTTCGAATAGGTTCGGTTTTCATCGACTCTTATTGAACCGAACCGAACCAAAAGTAAATTATTGCAAGTATAGTGTAGTGTCGTGCACTACTCAAAGTGTTAGAATTAGCCAATTAGGTATTAATTTTTTTTTGTCAGACATTTCTAAATTTACATGAGTTCGACGTTTTCATTTCATCATAAAAAGAAATAGATAAATATTAAAAAGTACTTTGTGCGAATATAATTTAAATTAGGTTAAAGTATCATTTCAATTTCTGAAATTTTTAATGTATGATTTTAGTATTTATATTTTTAATGAATCTTAAATATATTCCTTCAAAATTAATTTGTATTGAAATTGACTTAATAATAATGATAATTTTCATTTTTAAAAAATAATATATAAATAATAGAAATGTTAATAGATAACAAAAAGTTAAAAAAAAATCAAGAAAAATTAGTTTTAAGATTTATTAAAAGTAAATGATTAAAATTAGACATTTAAGTATAATAACTAAAATTGAACCAATTTTAAAGTAAAAGACTAAAATGATATTTTAATATATAAATTAGAGTATTTTAACTCAATTCAAAATGTGCAAAATATTTATTTATTAATTTAAATAATATTTTAATATTTGTTTGTTTTTATATTTTTTTCTCCCCAAAATATCCATCATCCATATGGTTATTTTTTCAAGATTTAGATGATTATTTACATCAGCATGTGGGGTAGTGGGCCCATAGACTAAAGTTTCATGTGGAGCAAGGCGTGGCGTGGGTGGGGCATATGCTCACGGATTTTCTATTTTTAGAATTTATGTTATCATTTGGTTTAAGAAAATTTTGATAAAAAAAATGTCAAACTAAAAATAAATAAACAGTATAATTAGTAAACAGAATATTGATAGACATAGATAAATTTCTGCATTTATCAGTGATAGATTTGTATCATTTCTATCACTGATAGACCTTTGATAGACCTCTATCAACATTTATCACAACTATCTAAAAATTTTGTTGTTTTATGTAAATAGTTTCTTATATTTTTCCATTCTTAAAAATTCCTGTTTGATTTAATACTCTTTGGTTGGTTTGGTTTACTATTGGGAAATTTTGATTGTTTTTTTATTTTAGAGTAAATCAAAATTGACCATCGAATCGAACTAACCCACCTACTTTTTATTATGGTTATTTTCGTTAGTTTATTAGGTTTTAATTTGCTACCCTATTTGAAGTAAGAATTGGTTAGTTTTCAGTTTTATGATATTTTTAGGTACATAGTAAAATGATTTTCACTTTCTAATTCACTATGTGGTAATAACTCAAAGTTATCCACATATATTTGCACATGATTATCTGATGTGAAACATGTTCCCAAATCCTTTATCTTTATCCTATTGAGCCTCTTATTTTCATAGTTGGATGCTATCTAGGCTACACTTACTTCTGTGTAGCCCAAACAATTACATAGTAAAAAATTGCTATATGACAATTTTCTTTTAAAAAAAATGAATTGTTTTATTTTATTTTACTATTTGCCAAATGAGAAATGCATAAAATTAGCTAGAGCCTAGAATGCACCTAATTATTATCCATTTTTATAATGTTATTTTATTTTCACTCTCAGATCTATCTAGATTTGATTTAAACATAATTTTACATATGTTATTCTTCATTATTTAACATAACTAACTCTCTCATTGGGTCATTTTTTATTTGATACAATAATATCATACAGTTCATCCAATAAGTTCTGAGCTTCATAAGCCATCAAATATGATCGTTGCGGTTCGGATCGCGCTCCTTCTCTGCGCCGTTGTCTATTTCGTGATTGGAATTTTCGGGTACTTGTTGTTTGGGGACTCATTAATGTCAGATATTCTTATGAACTTTGATGAGAGTGCTGATACAACAGGGGCTTTGTTGAATGATATAGTGAGATTAAGCTATGCTGTTCATCTCATGCTTGTTTTCCCTTTGCTAAACTTCCCATTGAGGTCCAATATTAATGAATTACTGTTCCCAAAAAAGGCTCCTTTGGGCACTGATCAAAGAAGGTTTCTGAGCATTACAGTGGCTTTGTTGGTCTTCTCCTACCTAGCTGCAATAGCATTCCCAAATATTTGGTACATCTTTCAATTCATGGGCTCAACTTCAGCTGCTTGCCTCGCCTTCATTTTCCCCGGCGCCATTGCTCTCAGGTACTTTGCTACTCTGATCTATTAGCTTAAGCTTTCTTTTAAGTTTGCAAATGATTTAGTGTGCTATTAACATAGAAGGTTATCAGTTCAAATCTCTATATATGTAATGTTACTTTGTTTCCATTTTTAGGTTGATCATCACTGAGTCTTGTGTTGACATAGAACATTTAAAATAAATCAAGTTTACACTTTCAAATCTAACATAAGAGTTGTGGGGTCTCTAAGATTTGTTTGAAAATCAAGTTTGTTAGGAAAGTAGTCAGTCTCTACTAATTTTCACTATAAATTTTAGAAACATATTTCACATGGTATATTGTTTGGTATGAAAATGATTAATATTGTTTTTTAACCAATTTCGTTAAAAATTAACTTCGAGAGACTAGATTTGTGATTTATTGAAATTATAGAAACTAAAATTAGACATGTGAGAGAACAAGGACTAAAATTGAACAAAATTCCAAAGTATAGGAACCAAAATAGTATTTTATAAAAAAAAATAAAAAATAAAAACATGGGTCAAATACATCTTTCCAAACAACTTAATATGCACACCCAACAGCCTATTTAGTTATTTATTTATTTATTTATTTTATTTTTTATAATATGTGGGTGGGAAAATCGAACCTGTGTATCAAAGTTTGATGATATAAGTTTAATGCAAGTTGTGTTATGCTCATTTTGGCATCTCTTTACTAATTAGAGTAGGAATACTAAATTCAAACATTTACTATACATAGATAGACTAAACTTCACATATCAACATATCAATCAACCATAAGTTCAGTTGTATTTTACTAAAATTTGTTTGGAAATGCAGGGATGTGAATGGAATAGCAACAAAGAAGGATAAAGTGGTTGCTTTAGGAATGGTAATTCTGGCAGTGGTGACCAGCATTATTGCCATTTCTATAAACATAAACAAGGCATTGAAAAGCAACCAGTAGAAATTATTAATTTGATTTTGAAGCTCCAATTATTTGTTTTTGAAGATGCTGATTGGTTGTATAGTTTGTAAGCACTCCCTCTTTGATCTCTCTCTTTCTCTCTCTCTTTTTATCCTTTTTAAGTTCAATTTTGTATATTTTCAAATTGCTTTATAAACCATGGTTGGATTCTTTAAATTTTTTTTGTATTGGCTTGTTCATTTTTGAGAAATTAATGGCAATAATAGCATAAAGACACTTAAATCTGAAA

mRNA sequence

GCACCACCAACCACCATACGCCGCGTAACAGAAGATTTAACGACATTAATCTTCTCCTTCTCTTCATCTTCTTCTTCAGCTTTATTCTCTCCAGCTTATCATCGTATTGCCGCCGAGATGTCGCCGGCGCCGGAAAACGTACCTCTACTGCCGGGTGCGAAGCCGTGCACGAGGACTGCGTCGGTCTCCGGCGCCGTGTTCAACGTCTCGACCAGTATAATTGGCGCTGGAATCATGTCGATTCCCTTTGCTCTAAAGGTTCTCGGCATAATCCCCGCCCTCGTCTTGATCGTGTTCGTCGCTTTCCTTACTGACATATCGGTGGAGCTTTTGCTTAGATTCACTCATTCCGGCGACTCGACGACCTACGCCGGCGTCATGAAGGAGTCGTTCGGCCCGATTGGTTCGATTGCTACGCAAGTTTGTATCATGATTACTAATCTCGGTTGCTTGATTATGTACCAGATTATAATAGGTAAGTTTCTTTCGGACTTTAGAGATCTCAATCGTCTCTGCTTGATTTTACTTGCTTTGCTGAAATTAGGGCTGGAATTTGGAAATTCTTCATATCTGATTGCCGTAACGGCCATAGATTTTAGGGAAACGGTTCGTGAAATCGCAAATTTTTTTCGATTCTCCAATTTTCCGATTGATTCTCGGGATGTGTTCTCGGGCAATAAAGAAGGGGGAAAAGTGCATTTAGGAGTTTTACAAGAATGGTTTGGAAATCACTGGTGGAACACCAGAGAGTTCTCGATCCTTTTCACTCTCGTCTTCATTCTACTTCCTCTGGTTCTTCTCCGACGAGTTGATTCATTGAGATTTAGTTCGTTTGTATCGGTTGTTCTTGCCGTCGTTTTCGTTGGGATAAGCTCTGTATTGGCGATCATGGCGATCGTTGAAGGCAAAACGAAGAGCACCAGATTGGTACCGGAGCTTGACGAAGAAACCTCCTTCCTCGACCTTTTCACAGCTGTTCCAGTACATATTCATCCAATAAGTTCTGAGCTTCATAAGCCATCAAATATGATCGTTGCGGTTCGGATCGCGCTCCTTCTCTGCGCCGTTGTCTATTTCGTGATTGGAATTTTCGGGTACTTGTTGTTTGGGGACTCATTAATGTCAGATATTCTTATGAACTTTGATGAGAGTGCTGATACAACAGGGGCTTTGTTGAATGATATAGTGAGATTAAGCTATGCTGTTCATCTCATGCTTGTTTTCCCTTTGCTAAACTTCCCATTGAGGTCCAATATTAATGAATTACTGTTCCCAAAAAAGGCTCCTTTGGGCACTGATCAAAGAAGGTTTCTGAGCATTACAGTGGCTTTGTTGGTCTTCTCCTACCTAGCTGCAATAGCATTCCCAAATATTTGGTACATCTTTCAATTCATGGGCTCAACTTCAGCTGCTTGCCTCGCCTTCATTTTCCCCGGCGCCATTGCTCTCAGGGATGTGAATGGAATAGCAACAAAGAAGGATAAAGTGGTTGCTTTAGGAATGGTAATTCTGGCAGTGGTGACCAGCATTATTGCCATTTCTATAAACATAAACAAGGCATTGAAAAGCAACCAGTAGAAATTATTAATTTGATTTTGAAGCTCCAATTATTTGTTTTTGAAGATGCTGATTGGTTGTATAGTTTGTAAGCACTCCCTCTTTGATCTCTCTCTTTCTCTCTCTCTTTTTATCCTTTTTAAGTTCAATTTTGTATATTTTCAAATTGCTTTATAAACCATGGTTGGATTCTTTAAATTTTTTTTGTATTGGCTTGTTCATTTTTGAGAAATTAATGGCAATAATAGCATAAAGACACTTAAATCTGAAA

Coding sequence (CDS)

GCACCACCAACCACCATACGCCGCGTAACAGAAGATTTAACGACATTAATCTTCTCCTTCTCTTCATCTTCTTCTTCAGCTTTATTCTCTCCAGCTTATCATCGTATTGCCGCCGAGATGTCGCCGGCGCCGGAAAACGTACCTCTACTGCCGGGTGCGAAGCCGTGCACGAGGACTGCGTCGGTCTCCGGCGCCGTGTTCAACGTCTCGACCAGTATAATTGGCGCTGGAATCATGTCGATTCCCTTTGCTCTAAAGGTTCTCGGCATAATCCCCGCCCTCGTCTTGATCGTGTTCGTCGCTTTCCTTACTGACATATCGGTGGAGCTTTTGCTTAGATTCACTCATTCCGGCGACTCGACGACCTACGCCGGCGTCATGAAGGAGTCGTTCGGCCCGATTGGTTCGATTGCTACGCAAGTTTGTATCATGATTACTAATCTCGGTTGCTTGATTATGTACCAGATTATAATAGGTAAGTTTCTTTCGGACTTTAGAGATCTCAATCGTCTCTGCTTGATTTTACTTGCTTTGCTGAAATTAGGGCTGGAATTTGGAAATTCTTCATATCTGATTGCCGTAACGGCCATAGATTTTAGGGAAACGGTTCGTGAAATCGCAAATTTTTTTCGATTCTCCAATTTTCCGATTGATTCTCGGGATGTGTTCTCGGGCAATAAAGAAGGGGGAAAAGTGCATTTAGGAGTTTTACAAGAATGGTTTGGAAATCACTGGTGGAACACCAGAGAGTTCTCGATCCTTTTCACTCTCGTCTTCATTCTACTTCCTCTGGTTCTTCTCCGACGAGTTGATTCATTGAGATTTAGTTCGTTTGTATCGGTTGTTCTTGCCGTCGTTTTCGTTGGGATAAGCTCTGTATTGGCGATCATGGCGATCGTTGAAGGCAAAACGAAGAGCACCAGATTGGTACCGGAGCTTGACGAAGAAACCTCCTTCCTCGACCTTTTCACAGCTGTTCCAGTACATATTCATCCAATAAGTTCTGAGCTTCATAAGCCATCAAATATGATCGTTGCGGTTCGGATCGCGCTCCTTCTCTGCGCCGTTGTCTATTTCGTGATTGGAATTTTCGGGTACTTGTTGTTTGGGGACTCATTAATGTCAGATATTCTTATGAACTTTGATGAGAGTGCTGATACAACAGGGGCTTTGTTGAATGATATAGTGAGATTAAGCTATGCTGTTCATCTCATGCTTGTTTTCCCTTTGCTAAACTTCCCATTGAGGTCCAATATTAATGAATTACTGTTCCCAAAAAAGGCTCCTTTGGGCACTGATCAAAGAAGGTTTCTGAGCATTACAGTGGCTTTGTTGGTCTTCTCCTACCTAGCTGCAATAGCATTCCCAAATATTTGGTACATCTTTCAATTCATGGGCTCAACTTCAGCTGCTTGCCTCGCCTTCATTTTCCCCGGCGCCATTGCTCTCAGGGATGTGAATGGAATAGCAACAAAGAAGGATAAAGTGGTTGCTTTAGGAATGGTAATTCTGGCAGTGGTGACCAGCATTATTGCCATTTCTATAAACATAAACAAGGCATTGAAAAGCAACCAGTAG

Protein sequence

APPTTIRRVTEDLTTLIFSFSSSSSSALFSPAYHRIAAEMSPAPENVPLLPGAKPCTRTASVSGAVFNVSTSIIGAGIMSIPFALKVLGIIPALVLIVFVAFLTDISVELLLRFTHSGDSTTYAGVMKESFGPIGSIATQVCIMITNLGCLIMYQIIIGKFLSDFRDLNRLCLILLALLKLGLEFGNSSYLIAVTAIDFRETVREIANFFRFSNFPIDSRDVFSGNKEGGKVHLGVLQEWFGNHWWNTREFSILFTLVFILLPLVLLRRVDSLRFSSFVSVVLAVVFVGISSVLAIMAIVEGKTKSTRLVPELDEETSFLDLFTAVPVHIHPISSELHKPSNMIVAVRIALLLCAVVYFVIGIFGYLLFGDSLMSDILMNFDESADTTGALLNDIVRLSYAVHLMLVFPLLNFPLRSNINELLFPKKAPLGTDQRRFLSITVALLVFSYLAAIAFPNIWYIFQFMGSTSAACLAFIFPGAIALRDVNGIATKKDKVVALGMVILAVVTSIIAISININKALKSNQ
Homology
BLAST of Lsi04G010430 vs. ExPASy Swiss-Prot
Match: Q9LYM2 (Amino acid transporter AVT6C OS=Arabidopsis thaliana OX=3702 GN=AVT6C PE=2 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 5.2e-117
Identity = 247/482 (51.24%), Postives = 320/482 (66.39%), Query Frame = 0

Query: 49  LLPGAKPCT---RTASVSGAVFNVSTSIIGAGIMSIPFALKVLGIIPALVLIVFVAFLTD 108
           LLP  +P +     +S SG VFNVSTSIIGAGIMS+P A KVLGI+PA ++I  +A+L+ 
Sbjct: 9   LLPKQEPSSSENHGSSTSGIVFNVSTSIIGAGIMSMPAAFKVLGIVPAFLIITIIAWLST 68

Query: 109 ISVELLLRFTHSGDSTTYAGVMKESFGPIGSIATQVCIMITNLGCLIMYQIIIGKFLSDF 168
           ISV  L++ T +G+STTYAGVMKESFG  GSIA Q+  M+   GC+I++ IIIG      
Sbjct: 69  ISVGFLMKSTLAGESTTYAGVMKESFGKTGSIAVQIATMVATFGCMIIFSIIIG------ 128

Query: 169 RDLNRLCLILLALLKLGLEFGNSSYLIAVTAIDFRETVREIANFFRFSNFPIDSRDVFSG 228
                                                                  DV SG
Sbjct: 129 -------------------------------------------------------DVLSG 188

Query: 229 NKEGGKVHLGVLQEWFGNHWWNTREFSILFTLVFILLPLVLLRRVDSLRFSSFVSVVLAV 288
           N+ GG  H+GVLQEWFG++WWNTR F++LF   F+LLPLVL RRV+ L  SS VS +LAV
Sbjct: 189 NENGGPEHIGVLQEWFGSYWWNTRIFALLFVYGFVLLPLVLRRRVERLAISSAVSFLLAV 248

Query: 289 VFVGISSVLAIMAIVEGKTKSTRLVPELDEETSFLDLFTAVPV---------HIHPISSE 348
           +FV ISSVLAI A+V G+TK+ RL PEL    SF  LFTA PV         ++HPI  E
Sbjct: 249 LFVVISSVLAISALVNGQTKNPRLFPELSNGGSFWQLFTASPVIVTAFTFHFNVHPIGFE 308

Query: 349 LHKPSNMIVAVRIALLLCAVVYFVIGIFGYLLFGDSLMSDILMNFDESADTT-GALLNDI 408
           L  P  +I A +I+++LCA +YF  G+FGYLLFGD+ MSDIL+NFD+S+ ++ G+LLNDI
Sbjct: 309 LKDPLQVIPATKISVILCAAIYFATGLFGYLLFGDATMSDILVNFDQSSGSSIGSLLNDI 368

Query: 409 VRLSYAVHLMLVFPLLNFPLRSNINELLFPKKAPLGTDQRRFLSITVALLVFSYLAAIAF 468
           VRLSY +HLMLVFPLLNF LR+N++ELL+PKK  L  D +RF+ +T+ALL+  +L+AIA 
Sbjct: 369 VRLSYVLHLMLVFPLLNFSLRANLDELLYPKKPSLEKDTKRFIGLTLALLICCFLSAIAV 428

Query: 469 PNIWYIFQFMGSTSAACLAFIFPGAIALRDVNGIATKKDKVVALGMVILAVVTSIIAISI 518
           P+IWY FQFMGST    +AFIFP AI LR+++G++T ++K+VA  M++LAV TSIIAIS 
Sbjct: 429 PDIWYFFQFMGSTITVSIAFIFPAAIVLRNIHGVSTSREKIVAAIMLVLAVATSIIAIST 429

BLAST of Lsi04G010430 vs. ExPASy Swiss-Prot
Match: Q0WQJ3 (Amino acid transporter AVT6D OS=Arabidopsis thaliana OX=3702 GN=AVT6D PE=2 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 1.7e-115
Identity = 247/493 (50.10%), Postives = 326/493 (66.13%), Query Frame = 0

Query: 40  MSPAPENVPLLPGAKPCTRTA----SVSGAVFNVSTSIIGAGIMSIPFALKVLGIIPALV 99
           MSPA +  PLLP  +P + ++    S +GAVFN+STSI+GAGIM+IP A KVLG+IP+L 
Sbjct: 1   MSPAIK-APLLPNQEPSSSSSENHGSFAGAVFNISTSIVGAGIMAIPAAFKVLGVIPSLS 60

Query: 100 LIVFVAFLTDISVELLLRFTHSGDSTTYAGVMKESFGPIGSIATQVCIMITNLGCLIMYQ 159
           +IV +A+L+++S   L++ + +G+STTYAGVMKESFG  G++A  V  M+   G +I++ 
Sbjct: 61  IIVIIAWLSNVSAGFLMKSSLAGESTTYAGVMKESFGKSGAVAVTVVTMVVTFGSMIIFS 120

Query: 160 IIIGKFLSDFRDLNRLCLILLALLKLGLEFGNSSYLIAVTAIDFRETVREIANFFRFSNF 219
           IIIG                                                        
Sbjct: 121 IIIG-------------------------------------------------------- 180

Query: 220 PIDSRDVFSGNKEGGKVHLGVLQEWFGNHWWNTREFSILFTLVFILLPLVLLRRVDSLRF 279
                DV SGN++ G +HLG+LQEWFG+HWWNTR F +LF  VF+ LPLVL RRV+ L F
Sbjct: 181 -----DVISGNEKDGIIHLGLLQEWFGSHWWNTRFFGLLFIFVFLFLPLVLCRRVERLAF 240

Query: 280 SSFVSVVLAVVFVGISSVLAIMAIVEGKTKSTRLVPEL-DEETSFLDLFTAVPV------ 339
           SS +S +LA++FV ISSVLAI+A+V+GKTK  RL PEL D   SF  LFTA PV      
Sbjct: 241 SSAISFLLALLFVVISSVLAIIALVQGKTKPPRLFPELNDGGLSFFSLFTASPVIVTAFT 300

Query: 340 ---HIHPISSELHKPSNMIVAVRIALLLCAVVYFVIGIFGYLLFGDSLMSDILMNFDES- 399
              ++HP++ EL  P N++ A RI+++LCA +Y   G+F YLLFGDS M+D+LMNFD+S 
Sbjct: 301 FHFNVHPVAFELKDPLNVLSATRISVILCATIYSATGLFCYLLFGDSTMTDVLMNFDQST 360

Query: 400 ADTTGALLNDIVRLSYAVHLMLVFPLLNFPLRSNINELLFPKKAPLGTDQRRFLSITVAL 459
           + + G+LLNDIVRLSYA+HLMLVFPLLNF LR+N++ELLFP K  L  D +RF ++T  L
Sbjct: 361 SSSVGSLLNDIVRLSYAIHLMLVFPLLNFSLRANLDELLFPMKLSLVEDNKRFFALTFPL 420

Query: 460 LVFSYLAAIAFPNIWYIFQFMGSTSAACLAFIFPGAIALRDVNGIATKKDKVVALGMVIL 518
           L+  +L AIA P+IWY FQF+GSTS   +AFIFP AI LR+VNG +T ++K+VA  M++L
Sbjct: 421 LISCFLGAIAIPDIWYFFQFLGSTSTVSIAFIFPAAIVLRNVNGFSTLREKIVASVMLVL 431

BLAST of Lsi04G010430 vs. ExPASy Swiss-Prot
Match: F4KBM7 (Amino acid transporter AVT6B OS=Arabidopsis thaliana OX=3702 GN=AVT6B PE=3 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 2.3e-85
Identity = 194/466 (41.63%), Postives = 271/466 (58.15%), Query Frame = 0

Query: 60  ASVSGAVFNVSTSIIGAGIMSIPFALKVLGIIPALVLIVFVAFLTDISVELLLRFTHSGD 119
           AS SGAVFN++T+IIGAGIM++P  +K+LG+IP + +IV +AFLTD S+E LLRF++ G+
Sbjct: 35  ASFSGAVFNLATTIIGAGIMALPATMKILGLIPGITIIVLMAFLTDASIEFLLRFSNIGN 94

Query: 120 STTYAGVMKESFGPIGSIATQVCIMITNLGCLIMYQIIIGKFLSDFRDLNRLCLILLALL 179
             +Y GVM +SFG  G I  QV I+++N+G LI+Y IIIG                    
Sbjct: 95  QRSYGGVMDDSFGKCGRIMLQVSILVSNIGVLIVYMIIIG-------------------- 154

Query: 180 KLGLEFGNSSYLIAVTAIDFRETVREIANFFRFSNFPIDSRDVFSGNKEGGKVHLGVLQE 239
                                                    DV +G  E G  H G+L+ 
Sbjct: 155 -----------------------------------------DVLAGKNEYGIHHAGMLEG 214

Query: 240 WFGNHWWNTREFSILFTLVFILLPLVLLRRVDSLRFSSFVSVVLAVVFVGISSVLAIMAI 299
           WFG  WWN R F +L T + +  PL   +R+DSLRF+S +SV LAVVF+ I++ + I+ +
Sbjct: 215 WFGISWWNRRTFVLLVTTLTVFAPLTCFKRIDSLRFTSAISVALAVVFLVITAGITIIKL 274

Query: 300 VEGKTKSTRLVPELDEETSFLDLFTAVPV---------HIHPISSELHKPSNMIVAVRIA 359
                   RL+P + + +SF  LFT VPV         ++H I +EL  PS +   VR A
Sbjct: 275 FTDGLMMPRLLPNVTDLSSFWKLFTVVPVLVNAYICHYNVHSIQNELEDPSRIKPVVRSA 334

Query: 360 LLLCAVVYFVIGIFGYLLFGDSLMSDILMNFDESADTT-GALLNDIVRLSYAVHLMLVFP 419
           L +C+ VY +  +FGYLLFGD  + D+L NFD       G++LND VR SYA HLMLVFP
Sbjct: 335 LAMCSSVYVMTSLFGYLLFGDGTLDDVLANFDTDLGIPFGSVLNDAVRFSYAAHLMLVFP 394

Query: 420 LLNFPLRSNINELLFPKKAPLGTDQR--RFLSITVALLVFSYLAAIAFPNIWYIFQFMGS 479
           ++ +PLR NI+ L+FP   PL + +   RF SIT  L+   +L A   P+IW  FQF G+
Sbjct: 395 VVFYPLRINIDGLIFPTAPPLTSSESDLRFGSITAGLIAVIFLGANFIPSIWDAFQFTGA 439

Query: 480 TSAACLAFIFPGAIALRDVNGIATKKDKVVALGMVILAVVTSIIAI 514
           T+A C+ FIFP A+ L+D +  ATK+DK +A+ M++LAV ++ IAI
Sbjct: 455 TAAVCIGFIFPAAVILKDRHNQATKRDKTIAICMIVLAVFSNAIAI 439

BLAST of Lsi04G010430 vs. ExPASy Swiss-Prot
Match: Q9LI61 (Amino acid transporter AVT6A OS=Arabidopsis thaliana OX=3702 GN=AVT6A PE=2 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 6.8e-85
Identity = 194/475 (40.84%), Postives = 275/475 (57.89%), Query Frame = 0

Query: 60  ASVSGAVFNVSTSIIGAGIMSIPFALKVLGIIPALVLIVFVAFLTDISVELLLRFTHSGD 119
           AS SGAVFN++T+IIGAGIM++P  +K+LG+   + +IV +AFLTD S+E LLRF+ +G 
Sbjct: 43  ASFSGAVFNLATTIIGAGIMALPATMKILGLGLGITMIVVMAFLTDASIEFLLRFSKAGK 102

Query: 120 STTYAGVMKESFGPIGSIATQVCIMITNLGCLIMYQIIIGKFLSDFRDLNRLCLILLALL 179
           + +Y G+M  SFG  G I  QV +++ N+G LI+Y IIIG                    
Sbjct: 103 NRSYGGLMGGSFGNPGRILLQVAVLVNNIGVLIVYMIIIG-------------------- 162

Query: 180 KLGLEFGNSSYLIAVTAIDFRETVREIANFFRFSNFPIDSRDVFSGNKEGGKVHLGVLQE 239
                                                    DV +G  E G  H GVL+ 
Sbjct: 163 -----------------------------------------DVLAGKTEDGIHHFGVLEG 222

Query: 240 WFGNHWWNTREFSILFTLVFILLPLVLLRRVDSLRFSSFVSVVLAVVFVGISSVLAIMAI 299
           WFG+HWWN R   +L T + +  PL   +R+DSL+F+S +SV LAVVF+ I++ ++IM +
Sbjct: 223 WFGHHWWNGRAAILLITTLGVFAPLACFKRIDSLKFTSALSVALAVVFLIITAGISIMKL 282

Query: 300 VEGKTKSTRLVPELDEETSFLDLFTAVPV---------HIHPISSELHKPSNMIVAVRIA 359
           + G     RL+P++ + TSF +LFT VPV         ++H I +EL  PS +   VR A
Sbjct: 283 ISGGVAMPRLLPDVTDLTSFWNLFTVVPVLVTAFICHYNVHSIQNELEDPSQIRPVVRSA 342

Query: 360 LLLCAVVYFVIGIFGYLLFGDSLMSDILMNFDESADTT-GALLNDIVRLSYAVHLMLVFP 419
           L+LC+ VY +  IFG+LLFGD  + D+L NFD       G++LND VR+SYA+HLMLVFP
Sbjct: 343 LMLCSSVYIMTSIFGFLLFGDDTLDDVLANFDTDLGIPFGSILNDAVRVSYALHLMLVFP 402

Query: 420 LLNFPLRSNINELLFPKKAPLGTDQRRFLSITVALLVFSYLAAIAFPNIWYIFQFMGSTS 479
           ++ +PLR NI+ LLFP    L T   RF  +T  L+   +L A   P+IW  FQF G+T+
Sbjct: 403 IVFYPLRINIDGLLFPSARSLSTSNVRFGCLTAGLISVIFLGANFIPSIWDAFQFTGATA 456

Query: 480 AACLAFIFPGAIALRDVNGIATKKDKVVALGMVILAVVTSIIAISININKALKSN 525
           A CL FIFP +I L+D +  AT +D  +A+ M++LAV+++ IAI  +     K N
Sbjct: 463 AVCLGFIFPASIILKDRHDKATNRDTTLAIFMIVLAVLSNAIAIYSDAYALFKKN 456

BLAST of Lsi04G010430 vs. ExPASy Swiss-Prot
Match: Q9M8L9 (Amino acid transporter AVT6E OS=Arabidopsis thaliana OX=3702 GN=AVT6E PE=2 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 9.6e-63
Identity = 173/484 (35.74%), Postives = 252/484 (52.07%), Query Frame = 0

Query: 48  PLLPGAKPCTRTASVSGAVFNVSTSIIGAGIMSIPFALKVLGIIPALVLIVFVAFLTDIS 107
           PL+ G K   + + + GAVFN++TSIIGAGIM++P  +KVLG++   VLI+ +A L++IS
Sbjct: 64  PLVHG-KSSNQGSGIYGAVFNLTTSIIGAGIMALPATMKVLGLVLGFVLIILMALLSEIS 123

Query: 108 VELLLRFTHSGDSTTYAGVMKESFGPIGSIATQVCIMITNLGCLIMYQIIIGKFLSDFRD 167
           VELL+RF+    S +Y  V++ + G    + +++CI++ N G L++Y II+G        
Sbjct: 124 VELLVRFSVLYKSKSYGEVVQFALGKTARVLSEICIIVNNGGVLVVYLIIMG-------- 183

Query: 168 LNRLCLILLALLKLGLEFGNSSYLIAVTAIDFRETVREIANFFRFSNFPIDSRDVFSGNK 227
                                                                DV SG+ 
Sbjct: 184 -----------------------------------------------------DVMSGSL 243

Query: 228 EGGKVHLGVLQEWFGNHWWNTREFSILFTLVFILLPLVLLRRVDSLRFSSFVSVVLAVVF 287
                H+GVL +W GN +W+ R+  IL  +V  L PL  L ++DSL  +S  SV LAVVF
Sbjct: 244 H----HIGVLDQWLGNGFWDHRKVLILIVMVIFLAPLCALNKIDSLSVTSAASVALAVVF 303

Query: 288 VGISSVLAIMAIVEGKTKSTRLVPELDEETSFLDLFTAVPV---------HIHPISSEL- 347
           V +  V+A + ++EG     RL P+   + + LDL   +P+         ++ PI +EL 
Sbjct: 304 VVVCFVVATIKLIEGTIDPPRLSPDFGSKQAILDLLVVIPIMSNAYVCHFNVQPIYNELE 363

Query: 348 -HKPSNMIVAVRIALLLCAVVYFVIGIFGYLLFGDSLMSDILMNFDESADTT-GALLNDI 407
              P  M    RI   +C VVY    + GYLLFG    SDIL NFD+       + +N I
Sbjct: 364 GRSPHKMNRVGRITTAICVVVYASTAVSGYLLFGKDTESDILTNFDQDLGIRFSSAVNYI 423

Query: 408 VRLSYAVHLMLVFPLLNFPLRSNINELLFPKKAPLGTDQRRFLSITVALLVFSYLAAIAF 467
           VR+ Y +HL+LVFP+++F LR  +N LLF    PL   ++R L +TV LL   Y+ +   
Sbjct: 424 VRIGYILHLVLVFPVIHFSLRETVNTLLFEGSPPLSESKKRSLGLTVVLLALIYIGSTMI 481

Query: 468 PNIWYIFQFMGSTSAACLAFIFPGAIALR--DVNGIATKKDKVVALGMVILAVVTSIIAI 518
           PNIW  F+F G+TSA  L F FP  IALR    +   +  ++ V+  M+ILAVV SI+  
Sbjct: 484 PNIWTAFKFTGATSAVSLGFTFPALIALRLGKQSNSLSFVERSVSWLMLILAVVVSIVGT 481

BLAST of Lsi04G010430 vs. ExPASy TrEMBL
Match: A0A5D3DQ79 (Putative sodium-coupled neutral amino acid transporter 6 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold436G00380 PE=3 SV=1)

HSP 1 Score: 692.2 bits (1785), Expect = 1.7e-195
Identity = 390/493 (79.11%), Postives = 403/493 (81.74%), Query Frame = 0

Query: 40  MSPAPENVPLLPGAKPCTRTASVSGAVFNVSTSIIGAGIMSIPFALKVLGIIPALVLIVF 99
           MSPAPE VPLLP AKP  +TASVSGAVFNVSTSIIGAGIMSIPFALKVLGIIPALVLIVF
Sbjct: 1   MSPAPEQVPLLPPAKPSIKTASVSGAVFNVSTSIIGAGIMSIPFALKVLGIIPALVLIVF 60

Query: 100 VAFLTDISVELLLRFTHSGDSTTYAGVMKESFGPIGSIATQVCIMITNLGCLIMYQIIIG 159
           VAFLTDISVELLLRFTHSGDSTTYAGVMKESFGPIGSI TQVCIMITNLGCLIMYQIIIG
Sbjct: 61  VAFLTDISVELLLRFTHSGDSTTYAGVMKESFGPIGSITTQVCIMITNLGCLIMYQIIIG 120

Query: 160 KFLSDFRDLNRLCLILLALLKLGLEFGNSSYLIAVTAIDFRETVREIANFFRFSNFPIDS 219
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 220 RDVFSGNKEGGKVHLGVLQEWFGNHWWNTREFSILFTLVFILLPLVLLRRVDSLRFSSFV 279
            DV SGNKEGGKVH+GVLQEWFG+HWWNTREFSILFTLVFIL PLVL RRVDSLRFSSF+
Sbjct: 181 -DVLSGNKEGGKVHIGVLQEWFGHHWWNTREFSILFTLVFILFPLVLFRRVDSLRFSSFI 240

Query: 280 SVVLAVVFVGISSVLAIMAIVEGKTKSTRLVPELDEETSFLDLFTAVPV---------HI 339
           SVVLAVVF+GISSV+AIMAIVEGKTKSTRLVPE+DEETSF +LFTAVPV         ++
Sbjct: 241 SVVLAVVFIGISSVMAIMAIVEGKTKSTRLVPEIDEETSFFNLFTAVPVLVTSFCFHFNV 300

Query: 340 HPISSELHKPSNMIVAVRIALLLCAVVYFVIGIFGYLLFGDSLMSDILMNFDESADTTGA 399
           HPISSELHKPSNMI AVRIALLLCA+ YF IGIFGYLLFGDSLMSDILMNFDES DTTGA
Sbjct: 301 HPISSELHKPSNMITAVRIALLLCAIFYFTIGIFGYLLFGDSLMSDILMNFDESGDTTGA 360

Query: 400 LLNDIVRLSYAVHLMLVFPLLNFPLRSNINELLFPKKAPLGTDQRRFLSITVALLVFSYL 459
           LLND+VRLSYAVHLMLVFPLLNFPLRSNI EL FPKKA LGTDQRRFL IT ALLVFSYL
Sbjct: 361 LLNDVVRLSYAVHLMLVFPLLNFPLRSNIMELFFPKKASLGTDQRRFLGITTALLVFSYL 420

Query: 460 AAIAFPNIWYIFQFMGSTSAACLAFIFPGAIALRDVNGIATKKDKVVALGMVILAVVTSI 519
           AAIAFPNIWYIFQFMGSTSAACLAFIFPGAIALRDVNGIATKKDKVVALGMVILA+ TSI
Sbjct: 421 AAIAFPNIWYIFQFMGSTSAACLAFIFPGAIALRDVNGIATKKDKVVALGMVILALGTSI 432

Query: 520 IAISININKALKS 524
           IAISININ ALKS
Sbjct: 481 IAISININNALKS 432

BLAST of Lsi04G010430 vs. ExPASy TrEMBL
Match: A0A1S3C3V8 (probable sodium-coupled neutral amino acid transporter 6 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103496718 PE=3 SV=1)

HSP 1 Score: 692.2 bits (1785), Expect = 1.7e-195
Identity = 390/493 (79.11%), Postives = 403/493 (81.74%), Query Frame = 0

Query: 40  MSPAPENVPLLPGAKPCTRTASVSGAVFNVSTSIIGAGIMSIPFALKVLGIIPALVLIVF 99
           MSPAPE VPLLP AKP  +TASVSGAVFNVSTSIIGAGIMSIPFALKVLGIIPALVLIVF
Sbjct: 1   MSPAPEQVPLLPPAKPSIKTASVSGAVFNVSTSIIGAGIMSIPFALKVLGIIPALVLIVF 60

Query: 100 VAFLTDISVELLLRFTHSGDSTTYAGVMKESFGPIGSIATQVCIMITNLGCLIMYQIIIG 159
           VAFLTDISVELLLRFTHSGDSTTYAGVMKESFGPIGSI TQVCIMITNLGCLIMYQIIIG
Sbjct: 61  VAFLTDISVELLLRFTHSGDSTTYAGVMKESFGPIGSITTQVCIMITNLGCLIMYQIIIG 120

Query: 160 KFLSDFRDLNRLCLILLALLKLGLEFGNSSYLIAVTAIDFRETVREIANFFRFSNFPIDS 219
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 220 RDVFSGNKEGGKVHLGVLQEWFGNHWWNTREFSILFTLVFILLPLVLLRRVDSLRFSSFV 279
            DV SGNKEGGKVH+GVLQEWFG+HWWNTREFSILFTLVFIL PLVL RRVDSLRFSSF+
Sbjct: 181 -DVLSGNKEGGKVHIGVLQEWFGHHWWNTREFSILFTLVFILFPLVLFRRVDSLRFSSFI 240

Query: 280 SVVLAVVFVGISSVLAIMAIVEGKTKSTRLVPELDEETSFLDLFTAVPV---------HI 339
           SVVLAVVF+GISSV+AIMAIVEGKTKSTRLVPE+DEETSF +LFTAVPV         ++
Sbjct: 241 SVVLAVVFIGISSVMAIMAIVEGKTKSTRLVPEIDEETSFFNLFTAVPVLVTSFCFHFNV 300

Query: 340 HPISSELHKPSNMIVAVRIALLLCAVVYFVIGIFGYLLFGDSLMSDILMNFDESADTTGA 399
           HPISSELHKPSNMI AVRIALLLCA+ YF IGIFGYLLFGDSLMSDILMNFDES DTTGA
Sbjct: 301 HPISSELHKPSNMITAVRIALLLCAIFYFTIGIFGYLLFGDSLMSDILMNFDESGDTTGA 360

Query: 400 LLNDIVRLSYAVHLMLVFPLLNFPLRSNINELLFPKKAPLGTDQRRFLSITVALLVFSYL 459
           LLND+VRLSYAVHLMLVFPLLNFPLRSNI EL FPKKA LGTDQRRFL IT ALLVFSYL
Sbjct: 361 LLNDVVRLSYAVHLMLVFPLLNFPLRSNIMELFFPKKASLGTDQRRFLGITTALLVFSYL 420

Query: 460 AAIAFPNIWYIFQFMGSTSAACLAFIFPGAIALRDVNGIATKKDKVVALGMVILAVVTSI 519
           AAIAFPNIWYIFQFMGSTSAACLAFIFPGAIALRDVNGIATKKDKVVALGMVILA+ TSI
Sbjct: 421 AAIAFPNIWYIFQFMGSTSAACLAFIFPGAIALRDVNGIATKKDKVVALGMVILALGTSI 432

Query: 520 IAISININKALKS 524
           IAISININ ALKS
Sbjct: 481 IAISININNALKS 432

BLAST of Lsi04G010430 vs. ExPASy TrEMBL
Match: A0A0A0KQM4 (Amino acid transporter OS=Cucumis sativus OX=3659 GN=Csa_5G218780 PE=3 SV=1)

HSP 1 Score: 691.8 bits (1784), Expect = 2.2e-195
Identity = 390/493 (79.11%), Postives = 403/493 (81.74%), Query Frame = 0

Query: 40  MSPAPENVPLLPGAKPCTRTASVSGAVFNVSTSIIGAGIMSIPFALKVLGIIPALVLIVF 99
           MSPAPE VPLLP AKP  +TASVSGAVFNVSTSIIGAGIMSIPFALKVLGIIPALVLIVF
Sbjct: 1   MSPAPEQVPLLPAAKPSMKTASVSGAVFNVSTSIIGAGIMSIPFALKVLGIIPALVLIVF 60

Query: 100 VAFLTDISVELLLRFTHSGDSTTYAGVMKESFGPIGSIATQVCIMITNLGCLIMYQIIIG 159
           VAFLTDISVELLLRFTHSGDSTTYAGVMKESFGPIGSIATQVCIMITNLGCLIMYQIIIG
Sbjct: 61  VAFLTDISVELLLRFTHSGDSTTYAGVMKESFGPIGSIATQVCIMITNLGCLIMYQIIIG 120

Query: 160 KFLSDFRDLNRLCLILLALLKLGLEFGNSSYLIAVTAIDFRETVREIANFFRFSNFPIDS 219
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 220 RDVFSGNKEGGKVHLGVLQEWFGNHWWNTREFSILFTLVFILLPLVLLRRVDSLRFSSFV 279
            DV SGNKEGGKVHLGVLQEWFG+HWWNTREFSILFTLVFIL PLVL RRVDSLRFSSF+
Sbjct: 181 -DVLSGNKEGGKVHLGVLQEWFGHHWWNTREFSILFTLVFILFPLVLFRRVDSLRFSSFI 240

Query: 280 SVVLAVVFVGISSVLAIMAIVEGKTKSTRLVPELDEETSFLDLFTAVPV---------HI 339
           SVVLAVVF+GISSV+AIMAIVEGKTKSTRLVPELDEETSF DLFTAVPV         ++
Sbjct: 241 SVVLAVVFIGISSVMAIMAIVEGKTKSTRLVPELDEETSFFDLFTAVPVLVTAFCFHFNV 300

Query: 340 HPISSELHKPSNMIVAVRIALLLCAVVYFVIGIFGYLLFGDSLMSDILMNFDESADTTGA 399
           HPISSELHKPSNM  AVRIALLLCA+ YF IGIFGYLLFGDSLMSDILMNFDES DTTGA
Sbjct: 301 HPISSELHKPSNMTTAVRIALLLCAIFYFTIGIFGYLLFGDSLMSDILMNFDESGDTTGA 360

Query: 400 LLNDIVRLSYAVHLMLVFPLLNFPLRSNINELLFPKKAPLGTDQRRFLSITVALLVFSYL 459
           LLND+VRLSYAVHLMLVFPLLNFPLRSNI EL FPKKA  GTDQRRFL IT+ALLVFSYL
Sbjct: 361 LLNDVVRLSYAVHLMLVFPLLNFPLRSNIIELFFPKKASSGTDQRRFLGITLALLVFSYL 420

Query: 460 AAIAFPNIWYIFQFMGSTSAACLAFIFPGAIALRDVNGIATKKDKVVALGMVILAVVTSI 519
           AAIAFPNIWYIFQFMGSTSAACLAFIFPGAIALRDVNGIATKKDKVVALGMVILAV TSI
Sbjct: 421 AAIAFPNIWYIFQFMGSTSAACLAFIFPGAIALRDVNGIATKKDKVVALGMVILAVGTSI 432

Query: 520 IAISININKALKS 524
           +AISININ AL++
Sbjct: 481 VAISININNALEN 432

BLAST of Lsi04G010430 vs. ExPASy TrEMBL
Match: A0A6J1JW47 (amino acid transporter AVT6C-like OS=Cucurbita maxima OX=3661 GN=LOC111488403 PE=3 SV=1)

HSP 1 Score: 668.7 bits (1724), Expect = 2.0e-188
Identity = 383/518 (73.94%), Postives = 409/518 (78.96%), Query Frame = 0

Query: 16  LIFSFSSSSSSALFSPAYHRIAAEMSPAPENVPLLPGAKPCTRTASVSGAVFNVSTSIIG 75
           L F FSSSSS       YHR+AA MSP PEN PLL    P TRTASVSGAVFNV+TSIIG
Sbjct: 13  LFFFFSSSSS------VYHRLAAGMSPVPENTPLLSTVNPSTRTASVSGAVFNVATSIIG 72

Query: 76  AGIMSIPFALKVLGIIPALVLIVFVAFLTDISVELLLRFTHSGDSTTYAGVMKESFGPIG 135
           AGIMSIPFALKVLGIIPALVLIVFVAFLTDISVELLLRFTHSGDSTTYA +MKESFG IG
Sbjct: 73  AGIMSIPFALKVLGIIPALVLIVFVAFLTDISVELLLRFTHSGDSTTYASLMKESFGTIG 132

Query: 136 SIATQVCIMITNLGCLIMYQIIIGKFLSDFRDLNRLCLILLALLKLGLEFGNSSYLIAVT 195
           S+ATQVCIMITNLGCLIMYQIIIG                                    
Sbjct: 133 SVATQVCIMITNLGCLIMYQIIIG------------------------------------ 192

Query: 196 AIDFRETVREIANFFRFSNFPIDSRDVFSGNKEGGKVHLGVLQEWFGNHWWNTREFSILF 255
                                    DVFSGNKEGG+VHLG+LQEWFGNHWWN REFSILF
Sbjct: 193 -------------------------DVFSGNKEGGQVHLGILQEWFGNHWWNAREFSILF 252

Query: 256 TLVFILLPLVLLRRVDSLRFSSFVSVVLAVVFVGISSVLAIMAIVEGKTKSTRLVPELDE 315
           T VFILLPLVLLRRVDSLRFSSFVS VLAVVFVGISSV+AI+AIV+GKTKSTRLVPELD 
Sbjct: 253 TAVFILLPLVLLRRVDSLRFSSFVSFVLAVVFVGISSVMAIIAIVQGKTKSTRLVPELDN 312

Query: 316 ETSFLDLFTAVPV---------HIHPISSELHKPSNMIVAVRIALLLCAVVYFVIGIFGY 375
           ETSFL+LFTAVPV         ++HPISSEL KPSNMI AVRIALLLCAV+YF+IGIFGY
Sbjct: 313 ETSFLNLFTAVPVLVTAFTFHFNVHPISSELRKPSNMITAVRIALLLCAVIYFLIGIFGY 372

Query: 376 LLFGDSLMSDILMNFDESADTTGALLNDIVRLSYAVHLMLVFPLLNFPLRSNINELLFP- 435
           LLFGD LMSDILMNFD+S D TGA+LND+VRLSYA+HLMLVFP+ NFPLRSNINE LFP 
Sbjct: 373 LLFGDLLMSDILMNFDQSVDATGAMLNDVVRLSYAIHLMLVFPVQNFPLRSNINEFLFPN 432

Query: 436 KKAPLGTDQRRFLSITVALLVFSYLAAIAFPNIWYIFQFMGSTSAACLAFIFPGAIALRD 495
           K+A L TD+RRF+ IT+ALLVFSYLAAI FPNIWYIFQFMGSTSAACLAFIFPGAIALRD
Sbjct: 433 KEASLVTDERRFMGITMALLVFSYLAAITFPNIWYIFQFMGSTSAACLAFIFPGAIALRD 463

Query: 496 VNGIATKKDKVVALGMVILAVVTSIIAISININKALKS 524
           VNGIAT+KDKVVA  MVILA+VTSIIAI ININ ALK+
Sbjct: 493 VNGIATRKDKVVASIMVILAIVTSIIAIFININNALKN 463

BLAST of Lsi04G010430 vs. ExPASy TrEMBL
Match: A0A6J1GZ52 (amino acid transporter AVT6D-like OS=Cucurbita moschata OX=3662 GN=LOC111458196 PE=3 SV=1)

HSP 1 Score: 658.7 bits (1698), Expect = 2.1e-185
Identity = 375/494 (75.91%), Postives = 397/494 (80.36%), Query Frame = 0

Query: 40  MSPAPENVPLLPGAKPCTRTASVSGAVFNVSTSIIGAGIMSIPFALKVLGIIPALVLIVF 99
           MSP PEN+PLL    P TRTASVSGAVFNV+TSIIGAGIMSIPFALKVLGIIPALVLIVF
Sbjct: 1   MSPVPENIPLLSTVNPSTRTASVSGAVFNVATSIIGAGIMSIPFALKVLGIIPALVLIVF 60

Query: 100 VAFLTDISVELLLRFTHSGDSTTYAGVMKESFGPIGSIATQVCIMITNLGCLIMYQIIIG 159
           VAFLTDISVELLLRFTHSGDSTTYA +MKESFG IGS+ATQVCIMITNLGCLIMYQIIIG
Sbjct: 61  VAFLTDISVELLLRFTHSGDSTTYASLMKESFGTIGSVATQVCIMITNLGCLIMYQIIIG 120

Query: 160 KFLSDFRDLNRLCLILLALLKLGLEFGNSSYLIAVTAIDFRETVREIANFFRFSNFPIDS 219
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 220 RDVFSGNKEGGKVHLGVLQEWFGNHWWNTREFSILFTLVFILLPLVLLRRVDSLRFSSFV 279
            DVFSGNKEGG+VHLGVLQEWFGNHWWN REFSILFT VFILLPLVLLRRVDSLRFSSFV
Sbjct: 181 -DVFSGNKEGGQVHLGVLQEWFGNHWWNAREFSILFTAVFILLPLVLLRRVDSLRFSSFV 240

Query: 280 SVVLAVVFVGISSVLAIMAIVEGKTKSTRLVPELDEETSFLDLFTAVPV---------HI 339
           S VLAVVFVGISSV+AI+AIV+GKTKSTRLVPELD ETSFL+LFTAVPV         ++
Sbjct: 241 SFVLAVVFVGISSVMAIIAIVQGKTKSTRLVPELDSETSFLNLFTAVPVLVTAFTFHFNV 300

Query: 340 HPISSELHKPSNMIVAVRIALLLCAVVYFVIGIFGYLLFGDSLMSDILMNFDESADTTGA 399
           HPISSEL KPSNMI AVRIALLLCAV+YF+IGIFGYLLFGDSLMSDILMNFDES D TGA
Sbjct: 301 HPISSELRKPSNMITAVRIALLLCAVIYFLIGIFGYLLFGDSLMSDILMNFDESVDATGA 360

Query: 400 LLNDIVRLSYAVHLMLVFPLLNFPLRSNINELLFP-KKAPLGTDQRRFLSITVALLVFSY 459
           LLND+VRLSYA+HLMLVFP+ NFPLRSNINE LFP K+A L T++RRF+ IT+ALLVFSY
Sbjct: 361 LLNDVVRLSYAIHLMLVFPVQNFPLRSNINEFLFPNKEASLVTNERRFMGITMALLVFSY 420

Query: 460 LAAIAFPNIWYIFQFMGSTSAACLAFIFPGAIALRDVNGIATKKDKVVALGMVILAVVTS 519
           LAAI FPNIWYIFQFMGSTSAACLAFIFPGAIALRDVNGIATKKDKVVA  MV LAVVTS
Sbjct: 421 LAAITFPNIWYIFQFMGSTSAACLAFIFPGAIALRDVNGIATKKDKVVASIMVTLAVVTS 433

Query: 520 IIAISININKALKS 524
           IIAISININ ALK+
Sbjct: 481 IIAISININNALKN 433

BLAST of Lsi04G010430 vs. NCBI nr
Match: XP_038892972.1 (amino acid transporter AVT6C-like [Benincasa hispida])

HSP 1 Score: 729.9 bits (1883), Expect = 1.5e-206
Identity = 409/494 (82.79%), Postives = 419/494 (84.82%), Query Frame = 0

Query: 40  MSPAPENVPLLPGAKPCTRTASVSGAVFNVSTSIIGAGIMSIPFALKVLGIIPALVLIVF 99
           MSPAPENVPLLP AKPCTRTASVSGAVFNVSTSIIGAGIMSIPFALKVLGIIPALVLIVF
Sbjct: 1   MSPAPENVPLLPAAKPCTRTASVSGAVFNVSTSIIGAGIMSIPFALKVLGIIPALVLIVF 60

Query: 100 VAFLTDISVELLLRFTHSGDSTTYAGVMKESFGPIGSIATQVCIMITNLGCLIMYQIIIG 159
           VAFLTDISVELLLRFTHSGDSTTYAGVMKESFGPIGSIATQ+CIMITNLGCLIMYQIIIG
Sbjct: 61  VAFLTDISVELLLRFTHSGDSTTYAGVMKESFGPIGSIATQICIMITNLGCLIMYQIIIG 120

Query: 160 KFLSDFRDLNRLCLILLALLKLGLEFGNSSYLIAVTAIDFRETVREIANFFRFSNFPIDS 219
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 220 RDVFSGNKEGGKVHLGVLQEWFGNHWWNTREFSILFTLVFILLPLVLLRRVDSLRFSSFV 279
            DVFSGNKEGGKVHLGVLQEWFGNHWWN REFSILFTLVFILLPLVLLRRVDSLRFSSFV
Sbjct: 181 -DVFSGNKEGGKVHLGVLQEWFGNHWWNAREFSILFTLVFILLPLVLLRRVDSLRFSSFV 240

Query: 280 SVVLAVVFVGISSVLAIMAIVEGKTKSTRLVPELDEETSFLDLFTAVPV---------HI 339
           SVVLAVVFVGISSV+AIMAI EGKTKSTRLVPELDEETSF DLFTAVPV         ++
Sbjct: 241 SVVLAVVFVGISSVMAIMAIFEGKTKSTRLVPELDEETSFFDLFTAVPVLVTAFCFHFNV 300

Query: 340 HPISSELHKPSNMIVAVRIALLLCAVVYFVIGIFGYLLFGDSLMSDILMNFDESADTTGA 399
           HPISSELHKPSNMI+AVRIALLLCAVVYFVIGIFGYLLFGDSLMSDILMNFD+SADTTGA
Sbjct: 301 HPISSELHKPSNMIIAVRIALLLCAVVYFVIGIFGYLLFGDSLMSDILMNFDQSADTTGA 360

Query: 400 LLNDIVRLSYAVHLMLVFPLLNFPLRSNINELLFPKKAPLGTDQRRFLSITVALLVFSYL 459
           LLND+VRLSYAVHLMLVFPLLNFPLRSNINELLFPKKAPLGTDQ RFL+IT+ALLVFSYL
Sbjct: 361 LLNDVVRLSYAVHLMLVFPLLNFPLRSNINELLFPKKAPLGTDQTRFLAITMALLVFSYL 420

Query: 460 AAIAFPNIWYIFQFMGSTSAACLAFIFPGAIALRDVNGIATKKDKVVALGMVILAVVTSI 519
           AAIAFPNIWYIFQFMGSTSAACLAFIFPGAIALRDVNGIATKKDKVVALGMVILAV+TSI
Sbjct: 421 AAIAFPNIWYIFQFMGSTSAACLAFIFPGAIALRDVNGIATKKDKVVALGMVILAVLTSI 433

Query: 520 IAISININKALKSN 525
           IAISININKALKSN
Sbjct: 481 IAISININKALKSN 433

BLAST of Lsi04G010430 vs. NCBI nr
Match: XP_008456905.1 (PREDICTED: probable sodium-coupled neutral amino acid transporter 6 isoform X1 [Cucumis melo] >TYK25831.1 putative sodium-coupled neutral amino acid transporter 6 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 692.2 bits (1785), Expect = 3.5e-195
Identity = 390/493 (79.11%), Postives = 403/493 (81.74%), Query Frame = 0

Query: 40  MSPAPENVPLLPGAKPCTRTASVSGAVFNVSTSIIGAGIMSIPFALKVLGIIPALVLIVF 99
           MSPAPE VPLLP AKP  +TASVSGAVFNVSTSIIGAGIMSIPFALKVLGIIPALVLIVF
Sbjct: 1   MSPAPEQVPLLPPAKPSIKTASVSGAVFNVSTSIIGAGIMSIPFALKVLGIIPALVLIVF 60

Query: 100 VAFLTDISVELLLRFTHSGDSTTYAGVMKESFGPIGSIATQVCIMITNLGCLIMYQIIIG 159
           VAFLTDISVELLLRFTHSGDSTTYAGVMKESFGPIGSI TQVCIMITNLGCLIMYQIIIG
Sbjct: 61  VAFLTDISVELLLRFTHSGDSTTYAGVMKESFGPIGSITTQVCIMITNLGCLIMYQIIIG 120

Query: 160 KFLSDFRDLNRLCLILLALLKLGLEFGNSSYLIAVTAIDFRETVREIANFFRFSNFPIDS 219
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 220 RDVFSGNKEGGKVHLGVLQEWFGNHWWNTREFSILFTLVFILLPLVLLRRVDSLRFSSFV 279
            DV SGNKEGGKVH+GVLQEWFG+HWWNTREFSILFTLVFIL PLVL RRVDSLRFSSF+
Sbjct: 181 -DVLSGNKEGGKVHIGVLQEWFGHHWWNTREFSILFTLVFILFPLVLFRRVDSLRFSSFI 240

Query: 280 SVVLAVVFVGISSVLAIMAIVEGKTKSTRLVPELDEETSFLDLFTAVPV---------HI 339
           SVVLAVVF+GISSV+AIMAIVEGKTKSTRLVPE+DEETSF +LFTAVPV         ++
Sbjct: 241 SVVLAVVFIGISSVMAIMAIVEGKTKSTRLVPEIDEETSFFNLFTAVPVLVTSFCFHFNV 300

Query: 340 HPISSELHKPSNMIVAVRIALLLCAVVYFVIGIFGYLLFGDSLMSDILMNFDESADTTGA 399
           HPISSELHKPSNMI AVRIALLLCA+ YF IGIFGYLLFGDSLMSDILMNFDES DTTGA
Sbjct: 301 HPISSELHKPSNMITAVRIALLLCAIFYFTIGIFGYLLFGDSLMSDILMNFDESGDTTGA 360

Query: 400 LLNDIVRLSYAVHLMLVFPLLNFPLRSNINELLFPKKAPLGTDQRRFLSITVALLVFSYL 459
           LLND+VRLSYAVHLMLVFPLLNFPLRSNI EL FPKKA LGTDQRRFL IT ALLVFSYL
Sbjct: 361 LLNDVVRLSYAVHLMLVFPLLNFPLRSNIMELFFPKKASLGTDQRRFLGITTALLVFSYL 420

Query: 460 AAIAFPNIWYIFQFMGSTSAACLAFIFPGAIALRDVNGIATKKDKVVALGMVILAVVTSI 519
           AAIAFPNIWYIFQFMGSTSAACLAFIFPGAIALRDVNGIATKKDKVVALGMVILA+ TSI
Sbjct: 421 AAIAFPNIWYIFQFMGSTSAACLAFIFPGAIALRDVNGIATKKDKVVALGMVILALGTSI 432

Query: 520 IAISININKALKS 524
           IAISININ ALKS
Sbjct: 481 IAISININNALKS 432

BLAST of Lsi04G010430 vs. NCBI nr
Match: XP_004146452.1 (amino acid transporter AVT6C [Cucumis sativus] >KGN50712.1 hypothetical protein Csa_005867 [Cucumis sativus])

HSP 1 Score: 691.8 bits (1784), Expect = 4.5e-195
Identity = 390/493 (79.11%), Postives = 403/493 (81.74%), Query Frame = 0

Query: 40  MSPAPENVPLLPGAKPCTRTASVSGAVFNVSTSIIGAGIMSIPFALKVLGIIPALVLIVF 99
           MSPAPE VPLLP AKP  +TASVSGAVFNVSTSIIGAGIMSIPFALKVLGIIPALVLIVF
Sbjct: 1   MSPAPEQVPLLPAAKPSMKTASVSGAVFNVSTSIIGAGIMSIPFALKVLGIIPALVLIVF 60

Query: 100 VAFLTDISVELLLRFTHSGDSTTYAGVMKESFGPIGSIATQVCIMITNLGCLIMYQIIIG 159
           VAFLTDISVELLLRFTHSGDSTTYAGVMKESFGPIGSIATQVCIMITNLGCLIMYQIIIG
Sbjct: 61  VAFLTDISVELLLRFTHSGDSTTYAGVMKESFGPIGSIATQVCIMITNLGCLIMYQIIIG 120

Query: 160 KFLSDFRDLNRLCLILLALLKLGLEFGNSSYLIAVTAIDFRETVREIANFFRFSNFPIDS 219
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 220 RDVFSGNKEGGKVHLGVLQEWFGNHWWNTREFSILFTLVFILLPLVLLRRVDSLRFSSFV 279
            DV SGNKEGGKVHLGVLQEWFG+HWWNTREFSILFTLVFIL PLVL RRVDSLRFSSF+
Sbjct: 181 -DVLSGNKEGGKVHLGVLQEWFGHHWWNTREFSILFTLVFILFPLVLFRRVDSLRFSSFI 240

Query: 280 SVVLAVVFVGISSVLAIMAIVEGKTKSTRLVPELDEETSFLDLFTAVPV---------HI 339
           SVVLAVVF+GISSV+AIMAIVEGKTKSTRLVPELDEETSF DLFTAVPV         ++
Sbjct: 241 SVVLAVVFIGISSVMAIMAIVEGKTKSTRLVPELDEETSFFDLFTAVPVLVTAFCFHFNV 300

Query: 340 HPISSELHKPSNMIVAVRIALLLCAVVYFVIGIFGYLLFGDSLMSDILMNFDESADTTGA 399
           HPISSELHKPSNM  AVRIALLLCA+ YF IGIFGYLLFGDSLMSDILMNFDES DTTGA
Sbjct: 301 HPISSELHKPSNMTTAVRIALLLCAIFYFTIGIFGYLLFGDSLMSDILMNFDESGDTTGA 360

Query: 400 LLNDIVRLSYAVHLMLVFPLLNFPLRSNINELLFPKKAPLGTDQRRFLSITVALLVFSYL 459
           LLND+VRLSYAVHLMLVFPLLNFPLRSNI EL FPKKA  GTDQRRFL IT+ALLVFSYL
Sbjct: 361 LLNDVVRLSYAVHLMLVFPLLNFPLRSNIIELFFPKKASSGTDQRRFLGITLALLVFSYL 420

Query: 460 AAIAFPNIWYIFQFMGSTSAACLAFIFPGAIALRDVNGIATKKDKVVALGMVILAVVTSI 519
           AAIAFPNIWYIFQFMGSTSAACLAFIFPGAIALRDVNGIATKKDKVVALGMVILAV TSI
Sbjct: 421 AAIAFPNIWYIFQFMGSTSAACLAFIFPGAIALRDVNGIATKKDKVVALGMVILAVGTSI 432

Query: 520 IAISININKALKS 524
           +AISININ AL++
Sbjct: 481 VAISININNALEN 432

BLAST of Lsi04G010430 vs. NCBI nr
Match: XP_022991909.1 (amino acid transporter AVT6C-like [Cucurbita maxima])

HSP 1 Score: 668.7 bits (1724), Expect = 4.1e-188
Identity = 383/518 (73.94%), Postives = 409/518 (78.96%), Query Frame = 0

Query: 16  LIFSFSSSSSSALFSPAYHRIAAEMSPAPENVPLLPGAKPCTRTASVSGAVFNVSTSIIG 75
           L F FSSSSS       YHR+AA MSP PEN PLL    P TRTASVSGAVFNV+TSIIG
Sbjct: 13  LFFFFSSSSS------VYHRLAAGMSPVPENTPLLSTVNPSTRTASVSGAVFNVATSIIG 72

Query: 76  AGIMSIPFALKVLGIIPALVLIVFVAFLTDISVELLLRFTHSGDSTTYAGVMKESFGPIG 135
           AGIMSIPFALKVLGIIPALVLIVFVAFLTDISVELLLRFTHSGDSTTYA +MKESFG IG
Sbjct: 73  AGIMSIPFALKVLGIIPALVLIVFVAFLTDISVELLLRFTHSGDSTTYASLMKESFGTIG 132

Query: 136 SIATQVCIMITNLGCLIMYQIIIGKFLSDFRDLNRLCLILLALLKLGLEFGNSSYLIAVT 195
           S+ATQVCIMITNLGCLIMYQIIIG                                    
Sbjct: 133 SVATQVCIMITNLGCLIMYQIIIG------------------------------------ 192

Query: 196 AIDFRETVREIANFFRFSNFPIDSRDVFSGNKEGGKVHLGVLQEWFGNHWWNTREFSILF 255
                                    DVFSGNKEGG+VHLG+LQEWFGNHWWN REFSILF
Sbjct: 193 -------------------------DVFSGNKEGGQVHLGILQEWFGNHWWNAREFSILF 252

Query: 256 TLVFILLPLVLLRRVDSLRFSSFVSVVLAVVFVGISSVLAIMAIVEGKTKSTRLVPELDE 315
           T VFILLPLVLLRRVDSLRFSSFVS VLAVVFVGISSV+AI+AIV+GKTKSTRLVPELD 
Sbjct: 253 TAVFILLPLVLLRRVDSLRFSSFVSFVLAVVFVGISSVMAIIAIVQGKTKSTRLVPELDN 312

Query: 316 ETSFLDLFTAVPV---------HIHPISSELHKPSNMIVAVRIALLLCAVVYFVIGIFGY 375
           ETSFL+LFTAVPV         ++HPISSEL KPSNMI AVRIALLLCAV+YF+IGIFGY
Sbjct: 313 ETSFLNLFTAVPVLVTAFTFHFNVHPISSELRKPSNMITAVRIALLLCAVIYFLIGIFGY 372

Query: 376 LLFGDSLMSDILMNFDESADTTGALLNDIVRLSYAVHLMLVFPLLNFPLRSNINELLFP- 435
           LLFGD LMSDILMNFD+S D TGA+LND+VRLSYA+HLMLVFP+ NFPLRSNINE LFP 
Sbjct: 373 LLFGDLLMSDILMNFDQSVDATGAMLNDVVRLSYAIHLMLVFPVQNFPLRSNINEFLFPN 432

Query: 436 KKAPLGTDQRRFLSITVALLVFSYLAAIAFPNIWYIFQFMGSTSAACLAFIFPGAIALRD 495
           K+A L TD+RRF+ IT+ALLVFSYLAAI FPNIWYIFQFMGSTSAACLAFIFPGAIALRD
Sbjct: 433 KEASLVTDERRFMGITMALLVFSYLAAITFPNIWYIFQFMGSTSAACLAFIFPGAIALRD 463

Query: 496 VNGIATKKDKVVALGMVILAVVTSIIAISININKALKS 524
           VNGIAT+KDKVVA  MVILA+VTSIIAI ININ ALK+
Sbjct: 493 VNGIATRKDKVVASIMVILAIVTSIIAIFININNALKN 463

BLAST of Lsi04G010430 vs. NCBI nr
Match: XP_022956474.1 (amino acid transporter AVT6D-like [Cucurbita moschata])

HSP 1 Score: 658.7 bits (1698), Expect = 4.3e-185
Identity = 375/494 (75.91%), Postives = 397/494 (80.36%), Query Frame = 0

Query: 40  MSPAPENVPLLPGAKPCTRTASVSGAVFNVSTSIIGAGIMSIPFALKVLGIIPALVLIVF 99
           MSP PEN+PLL    P TRTASVSGAVFNV+TSIIGAGIMSIPFALKVLGIIPALVLIVF
Sbjct: 1   MSPVPENIPLLSTVNPSTRTASVSGAVFNVATSIIGAGIMSIPFALKVLGIIPALVLIVF 60

Query: 100 VAFLTDISVELLLRFTHSGDSTTYAGVMKESFGPIGSIATQVCIMITNLGCLIMYQIIIG 159
           VAFLTDISVELLLRFTHSGDSTTYA +MKESFG IGS+ATQVCIMITNLGCLIMYQIIIG
Sbjct: 61  VAFLTDISVELLLRFTHSGDSTTYASLMKESFGTIGSVATQVCIMITNLGCLIMYQIIIG 120

Query: 160 KFLSDFRDLNRLCLILLALLKLGLEFGNSSYLIAVTAIDFRETVREIANFFRFSNFPIDS 219
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 220 RDVFSGNKEGGKVHLGVLQEWFGNHWWNTREFSILFTLVFILLPLVLLRRVDSLRFSSFV 279
            DVFSGNKEGG+VHLGVLQEWFGNHWWN REFSILFT VFILLPLVLLRRVDSLRFSSFV
Sbjct: 181 -DVFSGNKEGGQVHLGVLQEWFGNHWWNAREFSILFTAVFILLPLVLLRRVDSLRFSSFV 240

Query: 280 SVVLAVVFVGISSVLAIMAIVEGKTKSTRLVPELDEETSFLDLFTAVPV---------HI 339
           S VLAVVFVGISSV+AI+AIV+GKTKSTRLVPELD ETSFL+LFTAVPV         ++
Sbjct: 241 SFVLAVVFVGISSVMAIIAIVQGKTKSTRLVPELDSETSFLNLFTAVPVLVTAFTFHFNV 300

Query: 340 HPISSELHKPSNMIVAVRIALLLCAVVYFVIGIFGYLLFGDSLMSDILMNFDESADTTGA 399
           HPISSEL KPSNMI AVRIALLLCAV+YF+IGIFGYLLFGDSLMSDILMNFDES D TGA
Sbjct: 301 HPISSELRKPSNMITAVRIALLLCAVIYFLIGIFGYLLFGDSLMSDILMNFDESVDATGA 360

Query: 400 LLNDIVRLSYAVHLMLVFPLLNFPLRSNINELLFP-KKAPLGTDQRRFLSITVALLVFSY 459
           LLND+VRLSYA+HLMLVFP+ NFPLRSNINE LFP K+A L T++RRF+ IT+ALLVFSY
Sbjct: 361 LLNDVVRLSYAIHLMLVFPVQNFPLRSNINEFLFPNKEASLVTNERRFMGITMALLVFSY 420

Query: 460 LAAIAFPNIWYIFQFMGSTSAACLAFIFPGAIALRDVNGIATKKDKVVALGMVILAVVTS 519
           LAAI FPNIWYIFQFMGSTSAACLAFIFPGAIALRDVNGIATKKDKVVA  MV LAVVTS
Sbjct: 421 LAAITFPNIWYIFQFMGSTSAACLAFIFPGAIALRDVNGIATKKDKVVASIMVTLAVVTS 433

Query: 520 IIAISININKALKS 524
           IIAISININ ALK+
Sbjct: 481 IIAISININNALKN 433

BLAST of Lsi04G010430 vs. TAIR 10
Match: AT3G56200.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 422.9 bits (1086), Expect = 3.7e-118
Identity = 247/482 (51.24%), Postives = 320/482 (66.39%), Query Frame = 0

Query: 49  LLPGAKPCT---RTASVSGAVFNVSTSIIGAGIMSIPFALKVLGIIPALVLIVFVAFLTD 108
           LLP  +P +     +S SG VFNVSTSIIGAGIMS+P A KVLGI+PA ++I  +A+L+ 
Sbjct: 9   LLPKQEPSSSENHGSSTSGIVFNVSTSIIGAGIMSMPAAFKVLGIVPAFLIITIIAWLST 68

Query: 109 ISVELLLRFTHSGDSTTYAGVMKESFGPIGSIATQVCIMITNLGCLIMYQIIIGKFLSDF 168
           ISV  L++ T +G+STTYAGVMKESFG  GSIA Q+  M+   GC+I++ IIIG      
Sbjct: 69  ISVGFLMKSTLAGESTTYAGVMKESFGKTGSIAVQIATMVATFGCMIIFSIIIG------ 128

Query: 169 RDLNRLCLILLALLKLGLEFGNSSYLIAVTAIDFRETVREIANFFRFSNFPIDSRDVFSG 228
                                                                  DV SG
Sbjct: 129 -------------------------------------------------------DVLSG 188

Query: 229 NKEGGKVHLGVLQEWFGNHWWNTREFSILFTLVFILLPLVLLRRVDSLRFSSFVSVVLAV 288
           N+ GG  H+GVLQEWFG++WWNTR F++LF   F+LLPLVL RRV+ L  SS VS +LAV
Sbjct: 189 NENGGPEHIGVLQEWFGSYWWNTRIFALLFVYGFVLLPLVLRRRVERLAISSAVSFLLAV 248

Query: 289 VFVGISSVLAIMAIVEGKTKSTRLVPELDEETSFLDLFTAVPV---------HIHPISSE 348
           +FV ISSVLAI A+V G+TK+ RL PEL    SF  LFTA PV         ++HPI  E
Sbjct: 249 LFVVISSVLAISALVNGQTKNPRLFPELSNGGSFWQLFTASPVIVTAFTFHFNVHPIGFE 308

Query: 349 LHKPSNMIVAVRIALLLCAVVYFVIGIFGYLLFGDSLMSDILMNFDESADTT-GALLNDI 408
           L  P  +I A +I+++LCA +YF  G+FGYLLFGD+ MSDIL+NFD+S+ ++ G+LLNDI
Sbjct: 309 LKDPLQVIPATKISVILCAAIYFATGLFGYLLFGDATMSDILVNFDQSSGSSIGSLLNDI 368

Query: 409 VRLSYAVHLMLVFPLLNFPLRSNINELLFPKKAPLGTDQRRFLSITVALLVFSYLAAIAF 468
           VRLSY +HLMLVFPLLNF LR+N++ELL+PKK  L  D +RF+ +T+ALL+  +L+AIA 
Sbjct: 369 VRLSYVLHLMLVFPLLNFSLRANLDELLYPKKPSLEKDTKRFIGLTLALLICCFLSAIAV 428

Query: 469 PNIWYIFQFMGSTSAACLAFIFPGAIALRDVNGIATKKDKVVALGMVILAVVTSIIAISI 518
           P+IWY FQFMGST    +AFIFP AI LR+++G++T ++K+VA  M++LAV TSIIAIS 
Sbjct: 429 PDIWYFFQFMGSTITVSIAFIFPAAIVLRNIHGVSTSREKIVAAIMLVLAVATSIIAIST 429

BLAST of Lsi04G010430 vs. TAIR 10
Match: AT2G40420.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 417.9 bits (1073), Expect = 1.2e-116
Identity = 247/493 (50.10%), Postives = 326/493 (66.13%), Query Frame = 0

Query: 40  MSPAPENVPLLPGAKPCTRTA----SVSGAVFNVSTSIIGAGIMSIPFALKVLGIIPALV 99
           MSPA +  PLLP  +P + ++    S +GAVFN+STSI+GAGIM+IP A KVLG+IP+L 
Sbjct: 1   MSPAIK-APLLPNQEPSSSSSENHGSFAGAVFNISTSIVGAGIMAIPAAFKVLGVIPSLS 60

Query: 100 LIVFVAFLTDISVELLLRFTHSGDSTTYAGVMKESFGPIGSIATQVCIMITNLGCLIMYQ 159
           +IV +A+L+++S   L++ + +G+STTYAGVMKESFG  G++A  V  M+   G +I++ 
Sbjct: 61  IIVIIAWLSNVSAGFLMKSSLAGESTTYAGVMKESFGKSGAVAVTVVTMVVTFGSMIIFS 120

Query: 160 IIIGKFLSDFRDLNRLCLILLALLKLGLEFGNSSYLIAVTAIDFRETVREIANFFRFSNF 219
           IIIG                                                        
Sbjct: 121 IIIG-------------------------------------------------------- 180

Query: 220 PIDSRDVFSGNKEGGKVHLGVLQEWFGNHWWNTREFSILFTLVFILLPLVLLRRVDSLRF 279
                DV SGN++ G +HLG+LQEWFG+HWWNTR F +LF  VF+ LPLVL RRV+ L F
Sbjct: 181 -----DVISGNEKDGIIHLGLLQEWFGSHWWNTRFFGLLFIFVFLFLPLVLCRRVERLAF 240

Query: 280 SSFVSVVLAVVFVGISSVLAIMAIVEGKTKSTRLVPEL-DEETSFLDLFTAVPV------ 339
           SS +S +LA++FV ISSVLAI+A+V+GKTK  RL PEL D   SF  LFTA PV      
Sbjct: 241 SSAISFLLALLFVVISSVLAIIALVQGKTKPPRLFPELNDGGLSFFSLFTASPVIVTAFT 300

Query: 340 ---HIHPISSELHKPSNMIVAVRIALLLCAVVYFVIGIFGYLLFGDSLMSDILMNFDES- 399
              ++HP++ EL  P N++ A RI+++LCA +Y   G+F YLLFGDS M+D+LMNFD+S 
Sbjct: 301 FHFNVHPVAFELKDPLNVLSATRISVILCATIYSATGLFCYLLFGDSTMTDVLMNFDQST 360

Query: 400 ADTTGALLNDIVRLSYAVHLMLVFPLLNFPLRSNINELLFPKKAPLGTDQRRFLSITVAL 459
           + + G+LLNDIVRLSYA+HLMLVFPLLNF LR+N++ELLFP K  L  D +RF ++T  L
Sbjct: 361 SSSVGSLLNDIVRLSYAIHLMLVFPLLNFSLRANLDELLFPMKLSLVEDNKRFFALTFPL 420

Query: 460 LVFSYLAAIAFPNIWYIFQFMGSTSAACLAFIFPGAIALRDVNGIATKKDKVVALGMVIL 518
           L+  +L AIA P+IWY FQF+GSTS   +AFIFP AI LR+VNG +T ++K+VA  M++L
Sbjct: 421 LISCFLGAIAIPDIWYFFQFLGSTSTVSIAFIFPAAIVLRNVNGFSTLREKIVASVMLVL 431

BLAST of Lsi04G010430 vs. TAIR 10
Match: AT5G38820.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 317.8 bits (813), Expect = 1.7e-86
Identity = 194/466 (41.63%), Postives = 271/466 (58.15%), Query Frame = 0

Query: 60  ASVSGAVFNVSTSIIGAGIMSIPFALKVLGIIPALVLIVFVAFLTDISVELLLRFTHSGD 119
           AS SGAVFN++T+IIGAGIM++P  +K+LG+IP + +IV +AFLTD S+E LLRF++ G+
Sbjct: 35  ASFSGAVFNLATTIIGAGIMALPATMKILGLIPGITIIVLMAFLTDASIEFLLRFSNIGN 94

Query: 120 STTYAGVMKESFGPIGSIATQVCIMITNLGCLIMYQIIIGKFLSDFRDLNRLCLILLALL 179
             +Y GVM +SFG  G I  QV I+++N+G LI+Y IIIG                    
Sbjct: 95  QRSYGGVMDDSFGKCGRIMLQVSILVSNIGVLIVYMIIIG-------------------- 154

Query: 180 KLGLEFGNSSYLIAVTAIDFRETVREIANFFRFSNFPIDSRDVFSGNKEGGKVHLGVLQE 239
                                                    DV +G  E G  H G+L+ 
Sbjct: 155 -----------------------------------------DVLAGKNEYGIHHAGMLEG 214

Query: 240 WFGNHWWNTREFSILFTLVFILLPLVLLRRVDSLRFSSFVSVVLAVVFVGISSVLAIMAI 299
           WFG  WWN R F +L T + +  PL   +R+DSLRF+S +SV LAVVF+ I++ + I+ +
Sbjct: 215 WFGISWWNRRTFVLLVTTLTVFAPLTCFKRIDSLRFTSAISVALAVVFLVITAGITIIKL 274

Query: 300 VEGKTKSTRLVPELDEETSFLDLFTAVPV---------HIHPISSELHKPSNMIVAVRIA 359
                   RL+P + + +SF  LFT VPV         ++H I +EL  PS +   VR A
Sbjct: 275 FTDGLMMPRLLPNVTDLSSFWKLFTVVPVLVNAYICHYNVHSIQNELEDPSRIKPVVRSA 334

Query: 360 LLLCAVVYFVIGIFGYLLFGDSLMSDILMNFDESADTT-GALLNDIVRLSYAVHLMLVFP 419
           L +C+ VY +  +FGYLLFGD  + D+L NFD       G++LND VR SYA HLMLVFP
Sbjct: 335 LAMCSSVYVMTSLFGYLLFGDGTLDDVLANFDTDLGIPFGSVLNDAVRFSYAAHLMLVFP 394

Query: 420 LLNFPLRSNINELLFPKKAPLGTDQR--RFLSITVALLVFSYLAAIAFPNIWYIFQFMGS 479
           ++ +PLR NI+ L+FP   PL + +   RF SIT  L+   +L A   P+IW  FQF G+
Sbjct: 395 VVFYPLRINIDGLIFPTAPPLTSSESDLRFGSITAGLIAVIFLGANFIPSIWDAFQFTGA 439

Query: 480 TSAACLAFIFPGAIALRDVNGIATKKDKVVALGMVILAVVTSIIAI 514
           T+A C+ FIFP A+ L+D +  ATK+DK +A+ M++LAV ++ IAI
Sbjct: 455 TAAVCIGFIFPAAVILKDRHNQATKRDKTIAICMIVLAVFSNAIAI 439

BLAST of Lsi04G010430 vs. TAIR 10
Match: AT3G30390.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 316.2 bits (809), Expect = 4.8e-86
Identity = 194/475 (40.84%), Postives = 275/475 (57.89%), Query Frame = 0

Query: 60  ASVSGAVFNVSTSIIGAGIMSIPFALKVLGIIPALVLIVFVAFLTDISVELLLRFTHSGD 119
           AS SGAVFN++T+IIGAGIM++P  +K+LG+   + +IV +AFLTD S+E LLRF+ +G 
Sbjct: 43  ASFSGAVFNLATTIIGAGIMALPATMKILGLGLGITMIVVMAFLTDASIEFLLRFSKAGK 102

Query: 120 STTYAGVMKESFGPIGSIATQVCIMITNLGCLIMYQIIIGKFLSDFRDLNRLCLILLALL 179
           + +Y G+M  SFG  G I  QV +++ N+G LI+Y IIIG                    
Sbjct: 103 NRSYGGLMGGSFGNPGRILLQVAVLVNNIGVLIVYMIIIG-------------------- 162

Query: 180 KLGLEFGNSSYLIAVTAIDFRETVREIANFFRFSNFPIDSRDVFSGNKEGGKVHLGVLQE 239
                                                    DV +G  E G  H GVL+ 
Sbjct: 163 -----------------------------------------DVLAGKTEDGIHHFGVLEG 222

Query: 240 WFGNHWWNTREFSILFTLVFILLPLVLLRRVDSLRFSSFVSVVLAVVFVGISSVLAIMAI 299
           WFG+HWWN R   +L T + +  PL   +R+DSL+F+S +SV LAVVF+ I++ ++IM +
Sbjct: 223 WFGHHWWNGRAAILLITTLGVFAPLACFKRIDSLKFTSALSVALAVVFLIITAGISIMKL 282

Query: 300 VEGKTKSTRLVPELDEETSFLDLFTAVPV---------HIHPISSELHKPSNMIVAVRIA 359
           + G     RL+P++ + TSF +LFT VPV         ++H I +EL  PS +   VR A
Sbjct: 283 ISGGVAMPRLLPDVTDLTSFWNLFTVVPVLVTAFICHYNVHSIQNELEDPSQIRPVVRSA 342

Query: 360 LLLCAVVYFVIGIFGYLLFGDSLMSDILMNFDESADTT-GALLNDIVRLSYAVHLMLVFP 419
           L+LC+ VY +  IFG+LLFGD  + D+L NFD       G++LND VR+SYA+HLMLVFP
Sbjct: 343 LMLCSSVYIMTSIFGFLLFGDDTLDDVLANFDTDLGIPFGSILNDAVRVSYALHLMLVFP 402

Query: 420 LLNFPLRSNINELLFPKKAPLGTDQRRFLSITVALLVFSYLAAIAFPNIWYIFQFMGSTS 479
           ++ +PLR NI+ LLFP    L T   RF  +T  L+   +L A   P+IW  FQF G+T+
Sbjct: 403 IVFYPLRINIDGLLFPSARSLSTSNVRFGCLTAGLISVIFLGANFIPSIWDAFQFTGATA 456

Query: 480 AACLAFIFPGAIALRDVNGIATKKDKVVALGMVILAVVTSIIAISININKALKSN 525
           A CL FIFP +I L+D +  AT +D  +A+ M++LAV+++ IAI  +     K N
Sbjct: 463 AVCLGFIFPASIILKDRHDKATNRDTTLAIFMIVLAVLSNAIAIYSDAYALFKKN 456

BLAST of Lsi04G010430 vs. TAIR 10
Match: AT3G30390.2 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 316.2 bits (809), Expect = 4.8e-86
Identity = 194/475 (40.84%), Postives = 275/475 (57.89%), Query Frame = 0

Query: 60  ASVSGAVFNVSTSIIGAGIMSIPFALKVLGIIPALVLIVFVAFLTDISVELLLRFTHSGD 119
           AS SGAVFN++T+IIGAGIM++P  +K+LG+   + +IV +AFLTD S+E LLRF+ +G 
Sbjct: 43  ASFSGAVFNLATTIIGAGIMALPATMKILGLGLGITMIVVMAFLTDASIEFLLRFSKAGK 102

Query: 120 STTYAGVMKESFGPIGSIATQVCIMITNLGCLIMYQIIIGKFLSDFRDLNRLCLILLALL 179
           + +Y G+M  SFG  G I  QV +++ N+G LI+Y IIIG                    
Sbjct: 103 NRSYGGLMGGSFGNPGRILLQVAVLVNNIGVLIVYMIIIG-------------------- 162

Query: 180 KLGLEFGNSSYLIAVTAIDFRETVREIANFFRFSNFPIDSRDVFSGNKEGGKVHLGVLQE 239
                                                    DV +G  E G  H GVL+ 
Sbjct: 163 -----------------------------------------DVLAGKTEDGIHHFGVLEG 222

Query: 240 WFGNHWWNTREFSILFTLVFILLPLVLLRRVDSLRFSSFVSVVLAVVFVGISSVLAIMAI 299
           WFG+HWWN R   +L T + +  PL   +R+DSL+F+S +SV LAVVF+ I++ ++IM +
Sbjct: 223 WFGHHWWNGRAAILLITTLGVFAPLACFKRIDSLKFTSALSVALAVVFLIITAGISIMKL 282

Query: 300 VEGKTKSTRLVPELDEETSFLDLFTAVPV---------HIHPISSELHKPSNMIVAVRIA 359
           + G     RL+P++ + TSF +LFT VPV         ++H I +EL  PS +   VR A
Sbjct: 283 ISGGVAMPRLLPDVTDLTSFWNLFTVVPVLVTAFICHYNVHSIQNELEDPSQIRPVVRSA 342

Query: 360 LLLCAVVYFVIGIFGYLLFGDSLMSDILMNFDESADTT-GALLNDIVRLSYAVHLMLVFP 419
           L+LC+ VY +  IFG+LLFGD  + D+L NFD       G++LND VR+SYA+HLMLVFP
Sbjct: 343 LMLCSSVYIMTSIFGFLLFGDDTLDDVLANFDTDLGIPFGSILNDAVRVSYALHLMLVFP 402

Query: 420 LLNFPLRSNINELLFPKKAPLGTDQRRFLSITVALLVFSYLAAIAFPNIWYIFQFMGSTS 479
           ++ +PLR NI+ LLFP    L T   RF  +T  L+   +L A   P+IW  FQF G+T+
Sbjct: 403 IVFYPLRINIDGLLFPSARSLSTSNVRFGCLTAGLISVIFLGANFIPSIWDAFQFTGATA 456

Query: 480 AACLAFIFPGAIALRDVNGIATKKDKVVALGMVILAVVTSIIAISININKALKSN 525
           A CL FIFP +I L+D +  AT +D  +A+ M++LAV+++ IAI  +     K N
Sbjct: 463 AVCLGFIFPASIILKDRHDKATNRDTTLAIFMIVLAVLSNAIAIYSDAYALFKKN 456

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LYM25.2e-11751.24Amino acid transporter AVT6C OS=Arabidopsis thaliana OX=3702 GN=AVT6C PE=2 SV=1[more]
Q0WQJ31.7e-11550.10Amino acid transporter AVT6D OS=Arabidopsis thaliana OX=3702 GN=AVT6D PE=2 SV=1[more]
F4KBM72.3e-8541.63Amino acid transporter AVT6B OS=Arabidopsis thaliana OX=3702 GN=AVT6B PE=3 SV=1[more]
Q9LI616.8e-8540.84Amino acid transporter AVT6A OS=Arabidopsis thaliana OX=3702 GN=AVT6A PE=2 SV=1[more]
Q9M8L99.6e-6335.74Amino acid transporter AVT6E OS=Arabidopsis thaliana OX=3702 GN=AVT6E PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3DQ791.7e-19579.11Putative sodium-coupled neutral amino acid transporter 6 isoform X1 OS=Cucumis m... [more]
A0A1S3C3V81.7e-19579.11probable sodium-coupled neutral amino acid transporter 6 isoform X1 OS=Cucumis m... [more]
A0A0A0KQM42.2e-19579.11Amino acid transporter OS=Cucumis sativus OX=3659 GN=Csa_5G218780 PE=3 SV=1[more]
A0A6J1JW472.0e-18873.94amino acid transporter AVT6C-like OS=Cucurbita maxima OX=3661 GN=LOC111488403 PE... [more]
A0A6J1GZ522.1e-18575.91amino acid transporter AVT6D-like OS=Cucurbita moschata OX=3662 GN=LOC111458196 ... [more]
Match NameE-valueIdentityDescription
XP_038892972.11.5e-20682.79amino acid transporter AVT6C-like [Benincasa hispida][more]
XP_008456905.13.5e-19579.11PREDICTED: probable sodium-coupled neutral amino acid transporter 6 isoform X1 [... [more]
XP_004146452.14.5e-19579.11amino acid transporter AVT6C [Cucumis sativus] >KGN50712.1 hypothetical protein ... [more]
XP_022991909.14.1e-18873.94amino acid transporter AVT6C-like [Cucurbita maxima][more]
XP_022956474.14.3e-18575.91amino acid transporter AVT6D-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT3G56200.13.7e-11851.24Transmembrane amino acid transporter family protein [more]
AT2G40420.11.2e-11650.10Transmembrane amino acid transporter family protein [more]
AT5G38820.11.7e-8641.63Transmembrane amino acid transporter family protein [more]
AT3G30390.14.8e-8640.84Transmembrane amino acid transporter family protein [more]
AT3G30390.24.8e-8640.84Transmembrane amino acid transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013057Amino acid transporter, transmembrane domainPFAMPF01490Aa_transcoord: 59..169
e-value: 1.9E-18
score: 66.4
coord: 236..510
e-value: 2.1E-33
score: 115.6
NoneNo IPR availablePANTHERPTHR22950:SF323AMINO ACID TRANSPORTER AVT6Ccoord: 220..522
coord: 43..170
NoneNo IPR availablePANTHERPTHR22950AMINO ACID TRANSPORTERcoord: 220..522
coord: 43..170

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G010430.1Lsi04G010430.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0003333 amino acid transmembrane transport
biological_process GO:0009734 auxin-activated signaling pathway
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0015171 amino acid transmembrane transporter activity
molecular_function GO:0015293 symporter activity