Lag0035644 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0035644
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionReverse transcriptase
Locationchr3: 26255474 .. 26258452 (+)
RNA-Seq ExpressionLag0035644
SyntenyLag0035644
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCGTGGCGCAAAGCAGAGGAAATTAAGAAGTCCCACATCGGCAAAGCACGCAGTAGCGGACGTCAAGATCTGCTATAAATACAAGGCAATGGCTAAACGTCAATCATACTTACCTGGACGGGGTCAATGGGCGATCAAAAAGGCTCATGGCCTAGATTGGCGACCTCCATTGCACTTAAAGGAGGGGTGCCCGTCTAAGGTCTGTCCAAAGTGGCAGAGCCTACGTCATTATTTGTGGTAGTGGGGGCCTGCGTTCGCGCGGCCTCTTCCAATGCCTCTGTTTTAAGTGTTTACGGGCTTGCTTGCAAGGTTTTATTTTCTGAAAAATTAGGGCTCTGCACGAAGGTTCTATTTGATTTTTCCCTTATACCATATAACTTCATTTGAAAGTTGGGATAAAAATAAAAGATTTAGTATGTATACACACTTATAAAAAAAAAAAAAAAAAAAAAAAGGGAAAATACACTATAAAAAAAAATGGGGAAAATACACTTTTAGTCCCTAGGGTTGGTTAGGTTTCATTTTGGTTCCGAGTTTTCAAACTCAACCGTTTAGCCCCTGAAGTTTGGTTTTAGTTTCATTTTGGTCGCGTCGGTTTTGTGCAATTTTTTTATTAGGGTTTGATGTTTGGGAAAGAAGGAGATGAATGAAGAAAAGAAAAATAATAAAATAAAAAAAATATTTAATATATTATTTTATTTTAAAAAACAATATATATATATATATATATATATATAAACTAATGTCAGGTCAACCATCTGTAGAAAATTTGACGGAGGGACTAACGAAAATCAAACCTCATGAGCTAAATTGTTGAGTTTGAAAACTCGTGGATCAAAATGAAACCTAACCCAAACCTCAAGGACCAAAAGTGTATTTTTCCCTAAAATAAACAACTTAGGTTCTAGTACTAAAGAAATCATAAACTCCAATGTTTAAAACTCTTGGAAAATCATTTATGCAATAATTAAGCATTCTAAATTCAAAATACAACAAACAAAAGTAATCTCTGAGGCACATTGAAATTCGATACTATAATTTTAAACAACTAATTTTGTTGGTAATATAATTAAATTTTATTAAATACAAAATTTAAAATGTTGAGATCTTAAAATGTGTATTGCAATCTTTAAAAAATTATATTTACTTAAGAGAATTTATGATTTATCCAAAATTGTATTTACTTCAAAGAATTGATGTGGATGTAAATAACATTTGATTACATTATAAATATAAAAAATGATAGATAACAATACTTCTTTATCGACAAATATAAAATTTTGAATTCTCAAGGTTTTATTGTGACATGGTTCACGATCAAAATGTATGAATTCTCAAGGTTTTATTGTGACACGGTTCACGACCAAAATACATCAATTATATATTTCAAGGTTTTGTCAATTTTATATTTTCTTTTTATACATGGATCACACCTATACACAAATAAATATATGAACAGTTGCATGAATAACCATCTTCATATGAACAGTTGCACGTCGACATCAATTGTACCTATTGGACACAACCAAAATAACACCTTATTGTGACACGATTCAATAGTGTTTCAATTATATATTTTCTTCTTATACATGGATCGCACCTATACATGAATAGATACATCAATTCAATGTGTAACCCTCTCCATATGAACAGTCGCATCCCATCAACATCAATTGTACCTATTGGATAAAATTTCATATAGTTTGACATTTAACCATCTTTACACGAACAATCACATCTCGTCAACATTAATTGCACCTATTGGATTGAATTGACACTTATACTTGAAGGGTAGTAGATCATTCATTTTAAAATCATTGTCTTGCTGTGGTTGGTGTCGATGATACTGCTGCTCTTCTCTTGGTTGCCATAAGATTGCCCAAAACCTTATCGCTCTGATACCACTTGTTAGGGACTACCGGACAACCACATATCTCCACAATTGTATGATATTGTCCACTTTGGGCATAACCCTCATGACTTTGCTTTTGGATCACCCCAAAAGGCCTCATACCAATGGTGATGCTTGTCCTCCCTTATATACCCACGATCATCCCCATATCTAGCCGATGTGGGACTTTGGACGCACTCCCAACAATCCTCCCCTCGAACAAAGTACCACCGAGCCTCCCCTCAAATAGTCATCTATCCGTCCAACTCTCTAGGCTTCACCGCTTTACCGAGAGTTCTTGCTCTTATCTAGGACGACTCCTCTTCTCCGGAGTATACCCGCCCACCCAGAGCTCAACCACGGATCTCCCCATGACTATTAAGGCTCACAACTTCTTTGTTTGACACCCGAGGATTCTACCCAACATTGCTACCTCCGGGGCATGGCTCTGATACCAAACTTGGACACGACACAGATCCTCCCGGGGCAAAACTAAAGTACAGCACTACCAGCCATCCTCAAACCGATGGTCAAACAGAAGTAACAAACAGGACGTTGGACAATTTATTAAGGTGTCTAAGCGGGGACAAACCAGAGCAATGGGACATGTCATTGGCACAAGCCGAATTCGCCTACAACCACATGCGGAACAGAACGACAGGGAAGTCTCCATTTGAAATTGTCTATACAAAACTTCCTAGACTAACATTTGATCTAATACATATTCCCTCTTCGGTTGATCTTAGTACTGAAGCTGAGGAGATGGCTACTAGAATCACAGAGTTGCATAACCAAGTCCAAAACCACATTCAAGAGCAAACTGCTAAGTACAAAGAAGCTGCAGATAGGAAGAAGAGATTCAAAGAGTTTGAAGTTGGAGATCTCGTGATGATACATTTGAGGAAAAGCAGGCTCCCACCAGGAACTTACAACAAGCTCAAGAAAAGAAAGCTTGGGCCATTCCCTATATTGGAAAGATATGGCGAAAATGCTTATAAAATCAAACTACCAGAAGAATACAACATTAATCCTACATTTAATGTTGCTGACCTGTATTCTTTTCACCCCTGGATGAGTTTACTTTAG

mRNA sequence

ATGCGTGGCGCAAAGCAGAGGAAATTAAGAAGTCCCACATCGGCAAAGCACGCAGTAGCGGACGTCAAGATCTGCTATAAATACAAGGCAATGGCTAAACGTCAATCATACTTACCTGGACGGGGTCAATGGGCGATCAAAAAGGCTCATGGCCTAGATTGGCGACCTCCATTGCACTTAAAGGAGGGGTGCCCGTCTAAGGGACTACCGGACAACCACATATCTCCACAATTGTATGATATTGTCCACTTTGGGCATAACCCTCATGACTTTGCTTTTGGATCACCCCAAAAGGCCTCATACCAATGGCTCACAACTTCTTTGTTTGACACCCGAGGATTCTACCCAACATTGCTACCTCCGGGGCATGGCTCTGATACCAAACTTGGACACGACACAGATCCTCCCGGGGCAAAACTAAAGTACAGCACTACCAGCCATCCTCAAACCGATGGTCAAACAGAAGTAACAAACAGGACGTTGGACAATTTATTAAGGTGTCTAAGCGGGGACAAACCAGAGCAATGGGACATGTCATTGGCACAAGCCGAATTCGCCTACAACCACATGCGGAACAGAACGACAGGGAAGTCTCCATTTGAAATTGTCTATACAAAACTTCCTAGACTAACATTTGATCTAATACATATTCCCTCTTCGGTTGATCTTAGTACTGAAGCTGAGGAGATGGCTACTAGAATCACAGAGTTGCATAACCAAGTCCAAAACCACATTCAAGAGCAAACTGCTAAGTACAAAGAAGCTGCAGATAGGAAGAAGAGATTCAAAGAGTTTGAAGTTGGAGATCTCGTGATGATACATTTGAGGAAAAGCAGGCTCCCACCAGGAACTTACAACAAGCTCAAGAAAAGAAAGCTTGGGCCATTCCCTATATTGGAAAGATATGGCGAAAATGCTTATAAAATCAAACTACCAGAAGAATACAACATTAATCCTACATTTAATGTTGCTGACCTGTATTCTTTTCACCCCTGGATGAGTTTACTTTAG

Coding sequence (CDS)

ATGCGTGGCGCAAAGCAGAGGAAATTAAGAAGTCCCACATCGGCAAAGCACGCAGTAGCGGACGTCAAGATCTGCTATAAATACAAGGCAATGGCTAAACGTCAATCATACTTACCTGGACGGGGTCAATGGGCGATCAAAAAGGCTCATGGCCTAGATTGGCGACCTCCATTGCACTTAAAGGAGGGGTGCCCGTCTAAGGGACTACCGGACAACCACATATCTCCACAATTGTATGATATTGTCCACTTTGGGCATAACCCTCATGACTTTGCTTTTGGATCACCCCAAAAGGCCTCATACCAATGGCTCACAACTTCTTTGTTTGACACCCGAGGATTCTACCCAACATTGCTACCTCCGGGGCATGGCTCTGATACCAAACTTGGACACGACACAGATCCTCCCGGGGCAAAACTAAAGTACAGCACTACCAGCCATCCTCAAACCGATGGTCAAACAGAAGTAACAAACAGGACGTTGGACAATTTATTAAGGTGTCTAAGCGGGGACAAACCAGAGCAATGGGACATGTCATTGGCACAAGCCGAATTCGCCTACAACCACATGCGGAACAGAACGACAGGGAAGTCTCCATTTGAAATTGTCTATACAAAACTTCCTAGACTAACATTTGATCTAATACATATTCCCTCTTCGGTTGATCTTAGTACTGAAGCTGAGGAGATGGCTACTAGAATCACAGAGTTGCATAACCAAGTCCAAAACCACATTCAAGAGCAAACTGCTAAGTACAAAGAAGCTGCAGATAGGAAGAAGAGATTCAAAGAGTTTGAAGTTGGAGATCTCGTGATGATACATTTGAGGAAAAGCAGGCTCCCACCAGGAACTTACAACAAGCTCAAGAAAAGAAAGCTTGGGCCATTCCCTATATTGGAAAGATATGGCGAAAATGCTTATAAAATCAAACTACCAGAAGAATACAACATTAATCCTACATTTAATGTTGCTGACCTGTATTCTTTTCACCCCTGGATGAGTTTACTTTAG

Protein sequence

MRGAKQRKLRSPTSAKHAVADVKICYKYKAMAKRQSYLPGRGQWAIKKAHGLDWRPPLHLKEGCPSKGLPDNHISPQLYDIVHFGHNPHDFAFGSPQKASYQWLTTSLFDTRGFYPTLLPPGHGSDTKLGHDTDPPGAKLKYSTTSHPQTDGQTEVTNRTLDNLLRCLSGDKPEQWDMSLAQAEFAYNHMRNRTTGKSPFEIVYTKLPRLTFDLIHIPSSVDLSTEAEEMATRITELHNQVQNHIQEQTAKYKEAADRKKRFKEFEVGDLVMIHLRKSRLPPGTYNKLKKRKLGPFPILERYGENAYKIKLPEEYNINPTFNVADLYSFHPWMSLL
Homology
BLAST of Lag0035644 vs. NCBI nr
Match: TYJ99684.1 (serine/threonine-protein kinase TIO-like [Cucumis melo var. makuwa])

HSP 1 Score: 261.5 bits (667), Expect = 9.7e-66
Identity = 122/191 (63.87%), Postives = 157/191 (82.20%), Query Frame = 0

Query: 140 LKYSTTSHPQTDGQTEVTNRTLDNLLRCLSGDKPEQWDMSLAQAEFAYNHMRNRTTGKSP 199
           L Y+TT+HPQTDGQTEVTNRTL NL+RCLSG KP+QWD++LAQAEFA+N+M+NR+TGK P
Sbjct: 316 LNYNTTAHPQTDGQTEVTNRTLGNLIRCLSGTKPKQWDLALAQAEFAFNNMKNRSTGKCP 375

Query: 200 FEIVYTKLPRLTFDLIHIPSSVDLSTEAEEMATRITELHNQVQNHIQEQTAKYKEAADRK 259
           F+IVYTK PRLT DL  +P++V+L  EAE M   I +LH +V +H+ + T  YK++AD+K
Sbjct: 376 FKIVYTKQPRLTLDLTSLPTTVNLDKEAERMIGDIEKLHKEVYDHLVQTTYSYKKSADKK 435

Query: 260 KRFKEFEVGDLVMIHLRKSRLPPGTYNKLKKRKLGPFPILERYGENAYKIKLPEEYNINP 319
           +R  EF  GDLVMIHLRK+R P GTYNKLK +++GPFPILE+YG+NA+KI LP + +I+P
Sbjct: 436 RRQAEFSKGDLVMIHLRKNRFPTGTYNKLKDKQIGPFPILEKYGDNAFKIGLPLDIHIHP 495

Query: 320 TFNVADLYSFH 331
            FNVADL  +H
Sbjct: 496 VFNVADLKPYH 506

BLAST of Lag0035644 vs. NCBI nr
Match: KAA0054760.1 (DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa])

HSP 1 Score: 254.6 bits (649), Expect = 1.2e-63
Identity = 119/191 (62.30%), Postives = 155/191 (81.15%), Query Frame = 0

Query: 140 LKYSTTSHPQTDGQTEVTNRTLDNLLRCLSGDKPEQWDMSLAQAEFAYNHMRNRTTGKSP 199
           L Y+T +HPQTDGQTEVTNRTL NL+RCLSG KP+QWD++LAQAEFA+N+M+NR+TG+ P
Sbjct: 353 LNYNTIAHPQTDGQTEVTNRTLGNLIRCLSGTKPKQWDLALAQAEFAFNNMKNRSTGECP 412

Query: 200 FEIVYTKLPRLTFDLIHIPSSVDLSTEAEEMATRITELHNQVQNHIQEQTAKYKEAADRK 259
           F+IVYTK PRLT DL  +P++V+L  EAE M   I +LH +V +H+ + T  YK++AD+K
Sbjct: 413 FKIVYTKQPRLTLDLTSLPTTVNLDKEAERMIGDIEKLHKEVYDHLVQTTYSYKKSADKK 472

Query: 260 KRFKEFEVGDLVMIHLRKSRLPPGTYNKLKKRKLGPFPILERYGENAYKIKLPEEYNINP 319
           +R  EF  GDLVMIHLRK+R P GTYNKLK +++ PFPILE+YG+NA+KI LP + +I+P
Sbjct: 473 RRQAEFSKGDLVMIHLRKNRFPTGTYNKLKDKQIRPFPILEKYGDNAFKIGLPLDIHIHP 532

Query: 320 TFNVADLYSFH 331
            FNVADL  +H
Sbjct: 533 VFNVADLKPYH 543

BLAST of Lag0035644 vs. NCBI nr
Match: TYK26823.1 (serine/threonine-protein kinase TIO-like [Cucumis melo var. makuwa])

HSP 1 Score: 234.6 bits (597), Expect = 1.3e-57
Identity = 111/191 (58.12%), Postives = 149/191 (78.01%), Query Frame = 0

Query: 140 LKYSTTSHPQTDGQTEVTNRTLDNLLRCLSGDKPEQWDMSLAQAEFAYNHMRNRTTGKSP 199
           LKYSTT+HPQTDGQTEVTNRTL NL+RCLSG K +Q D++LAQ +F +N+M+NR+TGK P
Sbjct: 58  LKYSTTAHPQTDGQTEVTNRTLRNLIRCLSGTKLKQGDLALAQVKFTFNNMKNRSTGKCP 117

Query: 200 FEIVYTKLPRLTFDLIHIPSSVDLSTEAEEMATRITELHNQVQNHIQEQTAKYKEAADRK 259
           FE+VY K PRLTFDL  +P+++D +TE E+M  +I  LH +V +H+ E T  YK  AD+K
Sbjct: 118 FEVVYHKQPRLTFDLASLPTTMDTNTEVEKMIEKIENLHKEVHDHLMETTESYKRIADKK 177

Query: 260 KRFKEFEVGDLVMIHLRKSRLPPGTYNKLKKRKLGPFPILERYGENAYKIKLPEEYNINP 319
           +R   F  GDLV+IHL+K+R   GTYNKLK ++LGPF  LE++ +NA+KI+LP + +I+P
Sbjct: 178 RRQATFSEGDLVIIHLQKNRFLAGTYNKLKDKQLGPFRPLEKFEDNAFKIELPVDLHIHP 237

Query: 320 TFNVADLYSFH 331
            FNVADL  ++
Sbjct: 238 AFNVADLKLYY 248

BLAST of Lag0035644 vs. NCBI nr
Match: KAA0033503.1 (serine/threonine-protein kinase TIO-like [Cucumis melo var. makuwa])

HSP 1 Score: 234.6 bits (597), Expect = 1.3e-57
Identity = 111/191 (58.12%), Postives = 149/191 (78.01%), Query Frame = 0

Query: 140 LKYSTTSHPQTDGQTEVTNRTLDNLLRCLSGDKPEQWDMSLAQAEFAYNHMRNRTTGKSP 199
           LKYSTT+HPQTDGQTEVTNRTL NL+RCLSG K +Q D++LAQ +F +N+M+NR+TGK P
Sbjct: 58  LKYSTTAHPQTDGQTEVTNRTLRNLIRCLSGTKLKQGDLALAQVKFTFNNMKNRSTGKCP 117

Query: 200 FEIVYTKLPRLTFDLIHIPSSVDLSTEAEEMATRITELHNQVQNHIQEQTAKYKEAADRK 259
           FE+VY K PRLTFDL  +P+++D +TE E+M  +I  LH +V +H+ E T  YK  AD+K
Sbjct: 118 FEVVYHKQPRLTFDLASLPTTMDTNTEVEKMIEKIENLHKEVHDHLMETTESYKRIADKK 177

Query: 260 KRFKEFEVGDLVMIHLRKSRLPPGTYNKLKKRKLGPFPILERYGENAYKIKLPEEYNINP 319
           +R   F  GDLV+IHL+K+R   GTYNKLK ++LGPF  LE++ +NA+KI+LP + +I+P
Sbjct: 178 RRQATFSEGDLVIIHLQKNRFLAGTYNKLKDKQLGPFRPLEKFEDNAFKIELPVDLHIHP 237

Query: 320 TFNVADLYSFH 331
            FNVADL  ++
Sbjct: 238 AFNVADLKLYY 248

BLAST of Lag0035644 vs. NCBI nr
Match: KAG7586297.1 (Integrase catalytic core [Arabidopsis thaliana x Arabidopsis arenosa])

HSP 1 Score: 210.7 bits (535), Expect = 2.0e-50
Identity = 101/194 (52.06%), Postives = 135/194 (69.59%), Query Frame = 0

Query: 137  GAKLKYSTTSHPQTDGQTEVTNRTLDNLLRCLSGDKPEQWDMSLAQAEFAYNHMRNRTTG 196
            G  L  S+T+HPQTDGQTEVTNRTL N++R + GDKP+QWD++L Q EFAYN + +  TG
Sbjct: 1088 GTALNRSSTAHPQTDGQTEVTNRTLGNMVRSICGDKPKQWDLALPQIEFAYNSVVHSATG 1147

Query: 197  KSPFEIVYTKLPRLTFDLIHIPSSVDLSTEAEEMATRITELHNQVQNHIQEQTAKYKEAA 256
            KSPF +VYT +P+   DL+ +P +  +S  AE MA  I  +   V+  ++    K K AA
Sbjct: 1148 KSPFSLVYTSVPKHVVDLVKLPKAPGVSASAETMAEEILAVKEVVKAKLEATGKKNKVAA 1207

Query: 257  DRKKRFKEFEVGDLVMIHLRKSRLPPGTYNKLKKRKLGPFPILERYGENAYKIKLPEEYN 316
            D+++RFK F+ GD VM+ LRK R P GTYNKLK RK GPF +L +  +NAY + LPE  N
Sbjct: 1208 DKRRRFKVFKEGDDVMVFLRKERFPVGTYNKLKPRKYGPFKVLRKINDNAYVVALPESMN 1267

Query: 317  INPTFNVADLYSFH 331
            I+ TFNVAD++ +H
Sbjct: 1268 ISNTFNVADIHEYH 1281

BLAST of Lag0035644 vs. ExPASy Swiss-Prot
Match: Q9UR07 (Transposon Tf2-11 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-11 PE=3 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 3.8e-20
Identity = 63/193 (32.64%), Postives = 106/193 (54.92%), Query Frame = 0

Query: 140  LKYSTTSHPQTDGQTEVTNRTLDNLLRCLSGDKPEQWDMSLAQAEFAYNHMRNRTTGKSP 199
            +K+S    PQTDGQTE TN+T++ LLRC+    P  W   ++  + +YN+  +  T  +P
Sbjct: 1071 MKFSLPYRPQTDGQTERTNQTVEKLLRCVCSTHPNTWVDHISLVQQSYNNAIHSATQMTP 1130

Query: 200  FEIVYTKLPRLTFDLIHIPSSVDLSTEAEEMATRITELHNQVQNHIQEQTAKYKEAADRK 259
            FEIV+   P L+   + +PS    S + +E +    ++   V+ H+     K K+  D K
Sbjct: 1131 FEIVHRYSPALS--PLELPS---FSDKTDENSQETIQVFQTVKEHLNTNNIKMKKYFDMK 1190

Query: 260  -KRFKEFEVGDLVMIHLRKSRLPPGTYNKLKKRKLGPFPILERYGENAYKIKLPE--EYN 319
             +  +EF+ GDLVM+   K+     + NKL     GPF +L++ G N Y++ LP+  ++ 
Sbjct: 1191 IQEIEEFQPGDLVMVKRTKTGFLHKS-NKLAPSFAGPFYVLQKSGPNNYELDLPDSIKHM 1250

Query: 320  INPTFNVADLYSF 330
             + TF+V+ L  +
Sbjct: 1251 FSSTFHVSHLEKY 1257

BLAST of Lag0035644 vs. ExPASy Swiss-Prot
Match: P0CT41 (Transposon Tf2-12 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-12 PE=3 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 3.8e-20
Identity = 63/193 (32.64%), Postives = 106/193 (54.92%), Query Frame = 0

Query: 140  LKYSTTSHPQTDGQTEVTNRTLDNLLRCLSGDKPEQWDMSLAQAEFAYNHMRNRTTGKSP 199
            +K+S    PQTDGQTE TN+T++ LLRC+    P  W   ++  + +YN+  +  T  +P
Sbjct: 1071 MKFSLPYRPQTDGQTERTNQTVEKLLRCVCSTHPNTWVDHISLVQQSYNNAIHSATQMTP 1130

Query: 200  FEIVYTKLPRLTFDLIHIPSSVDLSTEAEEMATRITELHNQVQNHIQEQTAKYKEAADRK 259
            FEIV+   P L+   + +PS    S + +E +    ++   V+ H+     K K+  D K
Sbjct: 1131 FEIVHRYSPALS--PLELPS---FSDKTDENSQETIQVFQTVKEHLNTNNIKMKKYFDMK 1190

Query: 260  -KRFKEFEVGDLVMIHLRKSRLPPGTYNKLKKRKLGPFPILERYGENAYKIKLPE--EYN 319
             +  +EF+ GDLVM+   K+     + NKL     GPF +L++ G N Y++ LP+  ++ 
Sbjct: 1191 IQEIEEFQPGDLVMVKRTKTGFLHKS-NKLAPSFAGPFYVLQKSGPNNYELDLPDSIKHM 1250

Query: 320  INPTFNVADLYSF 330
             + TF+V+ L  +
Sbjct: 1251 FSSTFHVSHLEKY 1257

BLAST of Lag0035644 vs. ExPASy Swiss-Prot
Match: P0CT34 (Transposon Tf2-1 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-1 PE=3 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 3.8e-20
Identity = 63/193 (32.64%), Postives = 106/193 (54.92%), Query Frame = 0

Query: 140  LKYSTTSHPQTDGQTEVTNRTLDNLLRCLSGDKPEQWDMSLAQAEFAYNHMRNRTTGKSP 199
            +K+S    PQTDGQTE TN+T++ LLRC+    P  W   ++  + +YN+  +  T  +P
Sbjct: 1071 MKFSLPYRPQTDGQTERTNQTVEKLLRCVCSTHPNTWVDHISLVQQSYNNAIHSATQMTP 1130

Query: 200  FEIVYTKLPRLTFDLIHIPSSVDLSTEAEEMATRITELHNQVQNHIQEQTAKYKEAADRK 259
            FEIV+   P L+   + +PS    S + +E +    ++   V+ H+     K K+  D K
Sbjct: 1131 FEIVHRYSPALS--PLELPS---FSDKTDENSQETIQVFQTVKEHLNTNNIKMKKYFDMK 1190

Query: 260  -KRFKEFEVGDLVMIHLRKSRLPPGTYNKLKKRKLGPFPILERYGENAYKIKLPE--EYN 319
             +  +EF+ GDLVM+   K+     + NKL     GPF +L++ G N Y++ LP+  ++ 
Sbjct: 1191 IQEIEEFQPGDLVMVKRTKTGFLHKS-NKLAPSFAGPFYVLQKSGPNNYELDLPDSIKHM 1250

Query: 320  INPTFNVADLYSF 330
             + TF+V+ L  +
Sbjct: 1251 FSSTFHVSHLEKY 1257

BLAST of Lag0035644 vs. ExPASy Swiss-Prot
Match: P0CT35 (Transposon Tf2-2 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-2 PE=3 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 3.8e-20
Identity = 63/193 (32.64%), Postives = 106/193 (54.92%), Query Frame = 0

Query: 140  LKYSTTSHPQTDGQTEVTNRTLDNLLRCLSGDKPEQWDMSLAQAEFAYNHMRNRTTGKSP 199
            +K+S    PQTDGQTE TN+T++ LLRC+    P  W   ++  + +YN+  +  T  +P
Sbjct: 1071 MKFSLPYRPQTDGQTERTNQTVEKLLRCVCSTHPNTWVDHISLVQQSYNNAIHSATQMTP 1130

Query: 200  FEIVYTKLPRLTFDLIHIPSSVDLSTEAEEMATRITELHNQVQNHIQEQTAKYKEAADRK 259
            FEIV+   P L+   + +PS    S + +E +    ++   V+ H+     K K+  D K
Sbjct: 1131 FEIVHRYSPALS--PLELPS---FSDKTDENSQETIQVFQTVKEHLNTNNIKMKKYFDMK 1190

Query: 260  -KRFKEFEVGDLVMIHLRKSRLPPGTYNKLKKRKLGPFPILERYGENAYKIKLPE--EYN 319
             +  +EF+ GDLVM+   K+     + NKL     GPF +L++ G N Y++ LP+  ++ 
Sbjct: 1191 IQEIEEFQPGDLVMVKRTKTGFLHKS-NKLAPSFAGPFYVLQKSGPNNYELDLPDSIKHM 1250

Query: 320  INPTFNVADLYSF 330
             + TF+V+ L  +
Sbjct: 1251 FSSTFHVSHLEKY 1257

BLAST of Lag0035644 vs. ExPASy Swiss-Prot
Match: P0CT36 (Transposon Tf2-3 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-3 PE=1 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 3.8e-20
Identity = 63/193 (32.64%), Postives = 106/193 (54.92%), Query Frame = 0

Query: 140  LKYSTTSHPQTDGQTEVTNRTLDNLLRCLSGDKPEQWDMSLAQAEFAYNHMRNRTTGKSP 199
            +K+S    PQTDGQTE TN+T++ LLRC+    P  W   ++  + +YN+  +  T  +P
Sbjct: 1071 MKFSLPYRPQTDGQTERTNQTVEKLLRCVCSTHPNTWVDHISLVQQSYNNAIHSATQMTP 1130

Query: 200  FEIVYTKLPRLTFDLIHIPSSVDLSTEAEEMATRITELHNQVQNHIQEQTAKYKEAADRK 259
            FEIV+   P L+   + +PS    S + +E +    ++   V+ H+     K K+  D K
Sbjct: 1131 FEIVHRYSPALS--PLELPS---FSDKTDENSQETIQVFQTVKEHLNTNNIKMKKYFDMK 1190

Query: 260  -KRFKEFEVGDLVMIHLRKSRLPPGTYNKLKKRKLGPFPILERYGENAYKIKLPE--EYN 319
             +  +EF+ GDLVM+   K+     + NKL     GPF +L++ G N Y++ LP+  ++ 
Sbjct: 1191 IQEIEEFQPGDLVMVKRTKTGFLHKS-NKLAPSFAGPFYVLQKSGPNNYELDLPDSIKHM 1250

Query: 320  INPTFNVADLYSF 330
             + TF+V+ L  +
Sbjct: 1251 FSSTFHVSHLEKY 1257

BLAST of Lag0035644 vs. ExPASy TrEMBL
Match: A0A5D3BKX9 (Serine/threonine-protein kinase TIO-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold562G00780 PE=4 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 4.7e-66
Identity = 122/191 (63.87%), Postives = 157/191 (82.20%), Query Frame = 0

Query: 140 LKYSTTSHPQTDGQTEVTNRTLDNLLRCLSGDKPEQWDMSLAQAEFAYNHMRNRTTGKSP 199
           L Y+TT+HPQTDGQTEVTNRTL NL+RCLSG KP+QWD++LAQAEFA+N+M+NR+TGK P
Sbjct: 316 LNYNTTAHPQTDGQTEVTNRTLGNLIRCLSGTKPKQWDLALAQAEFAFNNMKNRSTGKCP 375

Query: 200 FEIVYTKLPRLTFDLIHIPSSVDLSTEAEEMATRITELHNQVQNHIQEQTAKYKEAADRK 259
           F+IVYTK PRLT DL  +P++V+L  EAE M   I +LH +V +H+ + T  YK++AD+K
Sbjct: 376 FKIVYTKQPRLTLDLTSLPTTVNLDKEAERMIGDIEKLHKEVYDHLVQTTYSYKKSADKK 435

Query: 260 KRFKEFEVGDLVMIHLRKSRLPPGTYNKLKKRKLGPFPILERYGENAYKIKLPEEYNINP 319
           +R  EF  GDLVMIHLRK+R P GTYNKLK +++GPFPILE+YG+NA+KI LP + +I+P
Sbjct: 436 RRQAEFSKGDLVMIHLRKNRFPTGTYNKLKDKQIGPFPILEKYGDNAFKIGLPLDIHIHP 495

Query: 320 TFNVADLYSFH 331
            FNVADL  +H
Sbjct: 496 VFNVADLKPYH 506

BLAST of Lag0035644 vs. ExPASy TrEMBL
Match: A0A5A7UHK1 (DNA/RNA polymerases superfamily protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold437G00380 PE=4 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 5.8e-64
Identity = 119/191 (62.30%), Postives = 155/191 (81.15%), Query Frame = 0

Query: 140 LKYSTTSHPQTDGQTEVTNRTLDNLLRCLSGDKPEQWDMSLAQAEFAYNHMRNRTTGKSP 199
           L Y+T +HPQTDGQTEVTNRTL NL+RCLSG KP+QWD++LAQAEFA+N+M+NR+TG+ P
Sbjct: 353 LNYNTIAHPQTDGQTEVTNRTLGNLIRCLSGTKPKQWDLALAQAEFAFNNMKNRSTGECP 412

Query: 200 FEIVYTKLPRLTFDLIHIPSSVDLSTEAEEMATRITELHNQVQNHIQEQTAKYKEAADRK 259
           F+IVYTK PRLT DL  +P++V+L  EAE M   I +LH +V +H+ + T  YK++AD+K
Sbjct: 413 FKIVYTKQPRLTLDLTSLPTTVNLDKEAERMIGDIEKLHKEVYDHLVQTTYSYKKSADKK 472

Query: 260 KRFKEFEVGDLVMIHLRKSRLPPGTYNKLKKRKLGPFPILERYGENAYKIKLPEEYNINP 319
           +R  EF  GDLVMIHLRK+R P GTYNKLK +++ PFPILE+YG+NA+KI LP + +I+P
Sbjct: 473 RRQAEFSKGDLVMIHLRKNRFPTGTYNKLKDKQIRPFPILEKYGDNAFKIGLPLDIHIHP 532

Query: 320 TFNVADLYSFH 331
            FNVADL  +H
Sbjct: 533 VFNVADLKPYH 543

BLAST of Lag0035644 vs. ExPASy TrEMBL
Match: A0A5A7SSR7 (Serine/threonine-protein kinase TIO-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold261G00420 PE=4 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 6.2e-58
Identity = 111/191 (58.12%), Postives = 149/191 (78.01%), Query Frame = 0

Query: 140 LKYSTTSHPQTDGQTEVTNRTLDNLLRCLSGDKPEQWDMSLAQAEFAYNHMRNRTTGKSP 199
           LKYSTT+HPQTDGQTEVTNRTL NL+RCLSG K +Q D++LAQ +F +N+M+NR+TGK P
Sbjct: 58  LKYSTTAHPQTDGQTEVTNRTLRNLIRCLSGTKLKQGDLALAQVKFTFNNMKNRSTGKCP 117

Query: 200 FEIVYTKLPRLTFDLIHIPSSVDLSTEAEEMATRITELHNQVQNHIQEQTAKYKEAADRK 259
           FE+VY K PRLTFDL  +P+++D +TE E+M  +I  LH +V +H+ E T  YK  AD+K
Sbjct: 118 FEVVYHKQPRLTFDLASLPTTMDTNTEVEKMIEKIENLHKEVHDHLMETTESYKRIADKK 177

Query: 260 KRFKEFEVGDLVMIHLRKSRLPPGTYNKLKKRKLGPFPILERYGENAYKIKLPEEYNINP 319
           +R   F  GDLV+IHL+K+R   GTYNKLK ++LGPF  LE++ +NA+KI+LP + +I+P
Sbjct: 178 RRQATFSEGDLVIIHLQKNRFLAGTYNKLKDKQLGPFRPLEKFEDNAFKIELPVDLHIHP 237

Query: 320 TFNVADLYSFH 331
            FNVADL  ++
Sbjct: 238 AFNVADLKLYY 248

BLAST of Lag0035644 vs. ExPASy TrEMBL
Match: A0A5D3DT79 (Serine/threonine-protein kinase TIO-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold260G00110 PE=4 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 6.2e-58
Identity = 111/191 (58.12%), Postives = 149/191 (78.01%), Query Frame = 0

Query: 140 LKYSTTSHPQTDGQTEVTNRTLDNLLRCLSGDKPEQWDMSLAQAEFAYNHMRNRTTGKSP 199
           LKYSTT+HPQTDGQTEVTNRTL NL+RCLSG K +Q D++LAQ +F +N+M+NR+TGK P
Sbjct: 58  LKYSTTAHPQTDGQTEVTNRTLRNLIRCLSGTKLKQGDLALAQVKFTFNNMKNRSTGKCP 117

Query: 200 FEIVYTKLPRLTFDLIHIPSSVDLSTEAEEMATRITELHNQVQNHIQEQTAKYKEAADRK 259
           FE+VY K PRLTFDL  +P+++D +TE E+M  +I  LH +V +H+ E T  YK  AD+K
Sbjct: 118 FEVVYHKQPRLTFDLASLPTTMDTNTEVEKMIEKIENLHKEVHDHLMETTESYKRIADKK 177

Query: 260 KRFKEFEVGDLVMIHLRKSRLPPGTYNKLKKRKLGPFPILERYGENAYKIKLPEEYNINP 319
           +R   F  GDLV+IHL+K+R   GTYNKLK ++LGPF  LE++ +NA+KI+LP + +I+P
Sbjct: 178 RRQATFSEGDLVIIHLQKNRFLAGTYNKLKDKQLGPFRPLEKFEDNAFKIELPVDLHIHP 237

Query: 320 TFNVADLYSFH 331
            FNVADL  ++
Sbjct: 238 AFNVADLKLYY 248

BLAST of Lag0035644 vs. ExPASy TrEMBL
Match: A0A6N2LVR1 (Uncharacterized protein OS=Salix viminalis OX=40686 GN=SVIM_LOCUS287486 PE=4 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 2.6e-56
Identity = 103/192 (53.65%), Postives = 146/192 (76.04%), Query Frame = 0

Query: 140  LKYSTTSHPQTDGQTEVTNRTLDNLLRCLSGDKPEQWDMSLAQAEFAYNHMRNRTTGKSP 199
            L +S+TSHPQTDGQTEV NRTL NL+RCLSG++P+QWD++LAQAEFAYN M NR+TGK+P
Sbjct: 1284 LNFSSTSHPQTDGQTEVVNRTLGNLIRCLSGERPKQWDLTLAQAEFAYNSMLNRSTGKTP 1343

Query: 200  FEIVYTKLPRLTFDLIHIPSSVDLSTEAEEMATRITELHNQVQNHIQEQTAKYKEAADRK 259
            F++VY + P+   DL+ +P    ++  AE MA R+  +  +V+ +++    KYK AAD+K
Sbjct: 1344 FQVVYCQPPKHALDLVPLPKLPGMNIAAEHMADRVRAIQEEVRKNLEASNEKYKAAADKK 1403

Query: 260  KRFKEFEVGDLVMIHLRKSRLPPGTYNKLKKRKLGPFPILERYGENAYKIKLPEEYNINP 319
            +R K F+ GDLVM++LRK R+P GT +KL  +K GP+ IL++  +NAY++ LP +  I+P
Sbjct: 1404 RRLKLFKEGDLVMVYLRKGRVPGGTLHKLSDKKHGPYQILQKINDNAYRVDLPADMTISP 1463

Query: 320  TFNVADLYSFHP 332
            TFNVADL+ +HP
Sbjct: 1464 TFNVADLFEYHP 1475

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TYJ99684.19.7e-6663.87serine/threonine-protein kinase TIO-like [Cucumis melo var. makuwa][more]
KAA0054760.11.2e-6362.30DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa][more]
TYK26823.11.3e-5758.12serine/threonine-protein kinase TIO-like [Cucumis melo var. makuwa][more]
KAA0033503.11.3e-5758.12serine/threonine-protein kinase TIO-like [Cucumis melo var. makuwa][more]
KAG7586297.12.0e-5052.06Integrase catalytic core [Arabidopsis thaliana x Arabidopsis arenosa][more]
Match NameE-valueIdentityDescription
Q9UR073.8e-2032.64Transposon Tf2-11 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24... [more]
P0CT413.8e-2032.64Transposon Tf2-12 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24... [more]
P0CT343.8e-2032.64Transposon Tf2-1 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 248... [more]
P0CT353.8e-2032.64Transposon Tf2-2 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 248... [more]
P0CT363.8e-2032.64Transposon Tf2-3 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 248... [more]
Match NameE-valueIdentityDescription
A0A5D3BKX94.7e-6663.87Serine/threonine-protein kinase TIO-like OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A5A7UHK15.8e-6462.30DNA/RNA polymerases superfamily protein OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A5A7SSR76.2e-5858.12Serine/threonine-protein kinase TIO-like OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A5D3DT796.2e-5858.12Serine/threonine-protein kinase TIO-like OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A6N2LVR12.6e-5653.65Uncharacterized protein OS=Salix viminalis OX=40686 GN=SVIM_LOCUS287486 PE=4 SV=... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 227..258
NoneNo IPR availablePANTHERPTHR24559TRANSPOSON TY3-I GAG-POL POLYPROTEINcoord: 139..331
NoneNo IPR availablePANTHERPTHR24559:SF367SUBFAMILY NOT NAMEDcoord: 139..331
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 134..250
e-value: 1.2E-21
score: 79.0
IPR001584Integrase, catalytic corePROSITEPS50994INTEGRASEcoord: 110..207
score: 10.045101
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 137..201

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0035644.1Lag0035644.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015074 DNA integration
biological_process GO:0016310 phosphorylation
molecular_function GO:0016301 kinase activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0008233 peptidase activity