CsaV3_7G030430 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_7G030430
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
DescriptiontRNA:m(4)X modification enzyme TRM13
Locationchr7: 19252012 .. 19254723 (+)
RNA-Seq ExpressionCsaV3_7G030430
SyntenyCsaV3_7G030430
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTGTCCACACACCCAAACAATAATTGTCCCGTTCAAAGCAATATCCATATTCCGTATGGGAATGAATATTTTCAGGTATCCAAACAGTGGGCATCTTCTTTGTAAGGTTTTAGGGTTTTAGGGTACGTTTCGAGCTTGAAGACATTGACAGTGAACCAAGAATTAGGGTTTTGAAATGGCTTCCCGTTGTCAGTTTTGGCTTCCAAAGAAGAACAGATTCTGTGCAGTTGCTCCAATTAACGATTCCAAGTACCACCATCTTTATTTCTCTGAAATTTAATGCTTATTTCAGTCGCTGGCTTCTCTTATAATTTTTCTTTCCTTTTCGAATTTTTTTGAAGATTTTGTGGCAATCACAGCTCCAGAGCTGATGGAGACATGGTTCCATGTCCTGTAGACCCGTCTCAGTAAGTTTAATAACTTTCTTTCTCATCTCTGTACTTAGTGTTCGTTAACTTTGATATATAGTTTCCAGTTTGGTAATAATTCTTAGCCTATTATGTTCTCTATATTCACTTCTCTAGCTCTGTGCTTCGAGAAAATCTTCAAGGACATGTGAAGAGGTGTCCATTATTGAAACAAACTCAATCCTTGGCTGGCCAACCGTTCTATCAAAAGGGCATCAATGCTGGGGAGGAGGATTCGATGTCGTCAAATGGACCGCATTCGGGTTTGTCAGATAGTATTTCCTCTGAAATGAAGAGGAATATTGTTTATGGTATGTCTGGAGATCAATTTCATCGCCTTCTTGGAAAGATTAGAGCTCTTCATGATCTGATATGCAAGGATATTCAAGACTCTTATAAGATAAAGGAAGCTTGCAATATGTGGATTAAAGGGGAAATAGATAGGTACTTAGTTGTTGTTGGCAATTAATGGCTTTCATGGGATTTAATGGGTTTTCTAAATTGTCCTCTGTTTTCTTTTGAGTAGGAAAATTCCGTTTCAAGAGAAACACGTTCTGCAGCAGGCGTCAATTCTCGGAAACTTGGACGAATTTGGAGTGCTGAGTAATTACGATGGAGGTGAACAACGTGAGGGTGACCGATCATATGGCAATGACGCTAATGTCGTCCCCGCTGTGGTTGAATTTGGAGCAGGAAGAGGCTACCTAACTCAAATGCTTGCAGATTGTTATGGTATCAAGAGGGTGTTTCTGGTTGAGCGGAAATCGTACAAACTGAAGGTTAAAATTCAAGATTTCGCCCTTATGTACGTCTAGATTTTTAGCATTTTGATTGGTTAAATTTTGCCAGTACTAAACTTCATTTGGATTTATACCTTTTGACTTTGTGTGTTTTGTTACGCCAATGCAACCCCTAGGCTGATCGATCGTTACGACAGAAAGAGAGCTTGATTTTAGAGCGTTTGAGAATAGACAGTAAGGTTTTCTCAAATTATGTTTTATGGCTTTTCGAAAACCATCTCTTAATATAGAGAGAAGTGCACGATAATCATATACCTGCATTCTTGTGTTAATTCCATCTGATTCTTGATCTTGATAACACAAGTACGGTTATATTCATTCTTACAGTTGAGGATCTAAACTTGAATGCTGTTGAATCTCTGAGGCATAATCCTTACTTAGCCATTGGTAAACATCTCTGTGGGCCTGCAACAGGTTGTTAGATCGCAGTATTGAATTTATTGTAAACTATAGTTCTTACCCGAAATAAAGCTGTTTGAACCACCTATTCCCAATTTTATTTCATAGATTTGGCTCTCAGATGTTGCCTTACCAAACAACCGAATCATGCTGTTACCGAACGGTGCAGAGATAAATCAAAATTGAGAGGTTTGGCGATAGCGACATGTTGCCATCACCTTTGTCAATGGAACCATTACACAAGTAAGCAATCCCTTTACATCTTGCGAATATATGCCTCAAATGAGGTATCTTTTTCCTTGACTTTAGAAGAAAGCAGTACTCAGTATTAGCTAAAGATGATCTTCTCTACAGATAAGAGATATTTATTAGAACTGGGAATCACAAAAGAAGAATTTCTTGCAATTACATGGTTCACTAGTTGGGCAGTGGATGCTAATCACAGTGAAGATATTTCAGATGTTACTGACTCAAAAACATTTTTTCAATCTAGGTAACCATTATCACGTGATCCATACAAAACCATTACTTTTACTGTTCATATTATAACCTTGTGATGCATGATAACAATTTTACATAAATTCTGCACAACTCCCATATCGTTGCAGTGAAAATGAAAGCGATGGGATGATTGGATGCATGGTAAAAGACATTGTAAAGAGTATGAATCCAGTGGAAAGGGCAGTCCTGGGATTTATGTGCAAGGAGATTATTGATATGGGAAGACTAATGTGGCTGAAGGAATGTGGATTGGAAACACAGCTTGTTAAGTATGTTCCATCTACCATTTCCCCTGAAAATCATTTACTAATCGCTAAATGCAGCAGACACATTTGTGCTACTTAATTTTATGATGCAATTTAAATTTCTATGGTTTATTGTGAGAATAAGTGGGGTTAGTATTTACTCTTGAACAGTTTGGTTGTTGTTTAAAGAAAAACAAACATTGAAAATCAAGGATGTGATGAAGAAAGAGCGCATTTTTTGTTTTGTTTCTTAAAATGTGTTTGATTATTGTTTTCAGATTGTGACTTCTCAATTTGAAACTTTAAATTAGGTAAAACATTTAGTTCTGAAATTTGAAAATAGTATGACAAAATTTGCG

mRNA sequence

ATGTCGTCAAATGGACCGCATTCGGGTTTGTCAGATAGTATTTCCTCTGAAATGAAGAGGAATATTGTTTATGGTATGTCTGGAGATCAATTTCATCGCCTTCTTGGAAAGATTAGAGCTCTTCATGATCTGATATGCAAGGATATTCAAGACTCTTATAAGATAAAGGAAGCTTGCAATATGTGGATTAAAGGGGAAATAGATAGGAAAATTCCGTTTCAAGAGAAACACGTTCTGCAGCAGGCGTCAATTCTCGGAAACTTGGACGAATTTGGAGTGCTGAGTAATTACGATGGAGGTGAACAACGTGAGGGTGACCGATCATATGGCAATGACGCTAATGTCGTCCCCGCTGTGGTTGAATTTGGAGCAGGAAGAGGCTACCTAACTCAAATGCTTGCAGATTGTTATGGTATCAAGAGGGTGTTTCTGGTTGAGCGGAAATCGTACAAACTGAAGGCTGATCGATCGTTACGACAGAAAGAGAGCTTGATTTTAGAGCGTTTGAGAATAGACATTGAGGATCTAAACTTGAATGCTGTTGAATCTCTGAGGCATAATCCTTACTTAGCCATTGGTAAACATCTCTGTGGGCCTGCAACAGATTTGGCTCTCAGATGTTGCCTTACCAAACAACCGAATCATGCTGTTACCGAACGGTGCAGAGATAAATCAAAATTGAGAGGTTTGGCGATAGCGACATGTTGCCATCACCTTTGTCAATGGAACCATTACACAAATAAGAGATATTTATTAGAACTGGGAATCACAAAAGAAGAATTTCTTGCAATTACATGGTTCACTAGTTGGGCAGTGGATGCTAATCACAGTGAAGATATTTCAGATGTTACTGACTCAAAAACATTTTTTCAATCTAGTGAAAATGAAAGCGATGGGATGATTGGATGCATGGTAAAAGACATTGTAAAGAGTATGAATCCAGTGGAAAGGGCAGTCCTGGGATTTATGTGCAAGGAGATTATTGATATGGGAAGACTAATGTGGCTGAAGGAATGTGGATTGGAAACACAGCTTGTTAAGTATGTTCCATCTACCATTTCCCCTGAAAATCATTTACTAATCGCTAAATGCAGCAGACACATTTGTGCTACTTAA

Coding sequence (CDS)

ATGTCGTCAAATGGACCGCATTCGGGTTTGTCAGATAGTATTTCCTCTGAAATGAAGAGGAATATTGTTTATGGTATGTCTGGAGATCAATTTCATCGCCTTCTTGGAAAGATTAGAGCTCTTCATGATCTGATATGCAAGGATATTCAAGACTCTTATAAGATAAAGGAAGCTTGCAATATGTGGATTAAAGGGGAAATAGATAGGAAAATTCCGTTTCAAGAGAAACACGTTCTGCAGCAGGCGTCAATTCTCGGAAACTTGGACGAATTTGGAGTGCTGAGTAATTACGATGGAGGTGAACAACGTGAGGGTGACCGATCATATGGCAATGACGCTAATGTCGTCCCCGCTGTGGTTGAATTTGGAGCAGGAAGAGGCTACCTAACTCAAATGCTTGCAGATTGTTATGGTATCAAGAGGGTGTTTCTGGTTGAGCGGAAATCGTACAAACTGAAGGCTGATCGATCGTTACGACAGAAAGAGAGCTTGATTTTAGAGCGTTTGAGAATAGACATTGAGGATCTAAACTTGAATGCTGTTGAATCTCTGAGGCATAATCCTTACTTAGCCATTGGTAAACATCTCTGTGGGCCTGCAACAGATTTGGCTCTCAGATGTTGCCTTACCAAACAACCGAATCATGCTGTTACCGAACGGTGCAGAGATAAATCAAAATTGAGAGGTTTGGCGATAGCGACATGTTGCCATCACCTTTGTCAATGGAACCATTACACAAATAAGAGATATTTATTAGAACTGGGAATCACAAAAGAAGAATTTCTTGCAATTACATGGTTCACTAGTTGGGCAGTGGATGCTAATCACAGTGAAGATATTTCAGATGTTACTGACTCAAAAACATTTTTTCAATCTAGTGAAAATGAAAGCGATGGGATGATTGGATGCATGGTAAAAGACATTGTAAAGAGTATGAATCCAGTGGAAAGGGCAGTCCTGGGATTTATGTGCAAGGAGATTATTGATATGGGAAGACTAATGTGGCTGAAGGAATGTGGATTGGAAACACAGCTTGTTAAGTATGTTCCATCTACCATTTCCCCTGAAAATCATTTACTAATCGCTAAATGCAGCAGACACATTTGTGCTACTTAA

Protein sequence

MSSNGPHSGLSDSISSEMKRNIVYGMSGDQFHRLLGKIRALHDLICKDIQDSYKIKEACNMWIKGEIDRKIPFQEKHVLQQASILGNLDEFGVLSNYDGGEQREGDRSYGNDANVVPAVVEFGAGRGYLTQMLADCYGIKRVFLVERKSYKLKADRSLRQKESLILERLRIDIEDLNLNAVESLRHNPYLAIGKHLCGPATDLALRCCLTKQPNHAVTERCRDKSKLRGLAIATCCHHLCQWNHYTNKRYLLELGITKEEFLAITWFTSWAVDANHSEDISDVTDSKTFFQSSENESDGMIGCMVKDIVKSMNPVERAVLGFMCKEIIDMGRLMWLKECGLETQLVKYVPSTISPENHLLIAKCSRHICAT*
Homology
BLAST of CsaV3_7G030430 vs. NCBI nr
Match: XP_011659414.1 (tRNA:m(4)X modification enzyme TRM13 homolog isoform X2 [Cucumis sativus] >KAE8646377.1 hypothetical protein Csa_016476 [Cucumis sativus])

HSP 1 Score: 761.9 bits (1966), Expect = 2.5e-216
Identity = 371/371 (100.00%), Postives = 371/371 (100.00%), Query Frame = 0

Query: 1   MSSNGPHSGLSDSISSEMKRNIVYGMSGDQFHRLLGKIRALHDLICKDIQDSYKIKEACN 60
           MSSNGPHSGLSDSISSEMKRNIVYGMSGDQFHRLLGKIRALHDLICKDIQDSYKIKEACN
Sbjct: 1   MSSNGPHSGLSDSISSEMKRNIVYGMSGDQFHRLLGKIRALHDLICKDIQDSYKIKEACN 60

Query: 61  MWIKGEIDRKIPFQEKHVLQQASILGNLDEFGVLSNYDGGEQREGDRSYGNDANVVPAVV 120
           MWIKGEIDRKIPFQEKHVLQQASILGNLDEFGVLSNYDGGEQREGDRSYGNDANVVPAVV
Sbjct: 61  MWIKGEIDRKIPFQEKHVLQQASILGNLDEFGVLSNYDGGEQREGDRSYGNDANVVPAVV 120

Query: 121 EFGAGRGYLTQMLADCYGIKRVFLVERKSYKLKADRSLRQKESLILERLRIDIEDLNLNA 180
           EFGAGRGYLTQMLADCYGIKRVFLVERKSYKLKADRSLRQKESLILERLRIDIEDLNLNA
Sbjct: 121 EFGAGRGYLTQMLADCYGIKRVFLVERKSYKLKADRSLRQKESLILERLRIDIEDLNLNA 180

Query: 181 VESLRHNPYLAIGKHLCGPATDLALRCCLTKQPNHAVTERCRDKSKLRGLAIATCCHHLC 240
           VESLRHNPYLAIGKHLCGPATDLALRCCLTKQPNHAVTERCRDKSKLRGLAIATCCHHLC
Sbjct: 181 VESLRHNPYLAIGKHLCGPATDLALRCCLTKQPNHAVTERCRDKSKLRGLAIATCCHHLC 240

Query: 241 QWNHYTNKRYLLELGITKEEFLAITWFTSWAVDANHSEDISDVTDSKTFFQSSENESDGM 300
           QWNHYTNKRYLLELGITKEEFLAITWFTSWAVDANHSEDISDVTDSKTFFQSSENESDGM
Sbjct: 241 QWNHYTNKRYLLELGITKEEFLAITWFTSWAVDANHSEDISDVTDSKTFFQSSENESDGM 300

Query: 301 IGCMVKDIVKSMNPVERAVLGFMCKEIIDMGRLMWLKECGLETQLVKYVPSTISPENHLL 360
           IGCMVKDIVKSMNPVERAVLGFMCKEIIDMGRLMWLKECGLETQLVKYVPSTISPENHLL
Sbjct: 301 IGCMVKDIVKSMNPVERAVLGFMCKEIIDMGRLMWLKECGLETQLVKYVPSTISPENHLL 360

Query: 361 IAKCSRHICAT 372
           IAKCSRHICAT
Sbjct: 361 IAKCSRHICAT 371

BLAST of CsaV3_7G030430 vs. NCBI nr
Match: XP_004135966.1 (tRNA:m(4)X modification enzyme TRM13 homolog isoform X1 [Cucumis sativus])

HSP 1 Score: 761.9 bits (1966), Expect = 2.5e-216
Identity = 371/371 (100.00%), Postives = 371/371 (100.00%), Query Frame = 0

Query: 1   MSSNGPHSGLSDSISSEMKRNIVYGMSGDQFHRLLGKIRALHDLICKDIQDSYKIKEACN 60
           MSSNGPHSGLSDSISSEMKRNIVYGMSGDQFHRLLGKIRALHDLICKDIQDSYKIKEACN
Sbjct: 88  MSSNGPHSGLSDSISSEMKRNIVYGMSGDQFHRLLGKIRALHDLICKDIQDSYKIKEACN 147

Query: 61  MWIKGEIDRKIPFQEKHVLQQASILGNLDEFGVLSNYDGGEQREGDRSYGNDANVVPAVV 120
           MWIKGEIDRKIPFQEKHVLQQASILGNLDEFGVLSNYDGGEQREGDRSYGNDANVVPAVV
Sbjct: 148 MWIKGEIDRKIPFQEKHVLQQASILGNLDEFGVLSNYDGGEQREGDRSYGNDANVVPAVV 207

Query: 121 EFGAGRGYLTQMLADCYGIKRVFLVERKSYKLKADRSLRQKESLILERLRIDIEDLNLNA 180
           EFGAGRGYLTQMLADCYGIKRVFLVERKSYKLKADRSLRQKESLILERLRIDIEDLNLNA
Sbjct: 208 EFGAGRGYLTQMLADCYGIKRVFLVERKSYKLKADRSLRQKESLILERLRIDIEDLNLNA 267

Query: 181 VESLRHNPYLAIGKHLCGPATDLALRCCLTKQPNHAVTERCRDKSKLRGLAIATCCHHLC 240
           VESLRHNPYLAIGKHLCGPATDLALRCCLTKQPNHAVTERCRDKSKLRGLAIATCCHHLC
Sbjct: 268 VESLRHNPYLAIGKHLCGPATDLALRCCLTKQPNHAVTERCRDKSKLRGLAIATCCHHLC 327

Query: 241 QWNHYTNKRYLLELGITKEEFLAITWFTSWAVDANHSEDISDVTDSKTFFQSSENESDGM 300
           QWNHYTNKRYLLELGITKEEFLAITWFTSWAVDANHSEDISDVTDSKTFFQSSENESDGM
Sbjct: 328 QWNHYTNKRYLLELGITKEEFLAITWFTSWAVDANHSEDISDVTDSKTFFQSSENESDGM 387

Query: 301 IGCMVKDIVKSMNPVERAVLGFMCKEIIDMGRLMWLKECGLETQLVKYVPSTISPENHLL 360
           IGCMVKDIVKSMNPVERAVLGFMCKEIIDMGRLMWLKECGLETQLVKYVPSTISPENHLL
Sbjct: 388 IGCMVKDIVKSMNPVERAVLGFMCKEIIDMGRLMWLKECGLETQLVKYVPSTISPENHLL 447

Query: 361 IAKCSRHICAT 372
           IAKCSRHICAT
Sbjct: 448 IAKCSRHICAT 458

BLAST of CsaV3_7G030430 vs. NCBI nr
Match: XP_008461388.1 (PREDICTED: tRNA:m(4)X modification enzyme TRM13 homolog isoform X1 [Cucumis melo])

HSP 1 Score: 723.8 bits (1867), Expect = 7.7e-205
Identity = 355/371 (95.69%), Postives = 362/371 (97.57%), Query Frame = 0

Query: 1   MSSNGPHSGLSDSISSEMKRNIVYGMSGDQFHRLLGKIRALHDLICKDIQDSYKIKEACN 60
           MS+NGP SGLSDSISSEMKRNIVYGMSGDQFHRLLGKIRALHDLICKDIQDSY+IKEACN
Sbjct: 88  MSANGPDSGLSDSISSEMKRNIVYGMSGDQFHRLLGKIRALHDLICKDIQDSYQIKEACN 147

Query: 61  MWIKGEIDRKIPFQEKHVLQQASILGNLDEFGVLSNYDGGEQREGDRSYGNDANVVPAVV 120
           MWIKGEIDRKIPFQEKHVLQQASILGNLDEFGVL NYDGG+QREG+RSYGNDANVVPAVV
Sbjct: 148 MWIKGEIDRKIPFQEKHVLQQASILGNLDEFGVLRNYDGGQQREGNRSYGNDANVVPAVV 207

Query: 121 EFGAGRGYLTQMLADCYGIKRVFLVERKSYKLKADRSLRQKESLILERLRIDIEDLNLNA 180
           EFGAGRGYLTQMLADCYGIKRVFLVERKSYKLKADRSLRQKESLILERLRIDIEDLNLNA
Sbjct: 208 EFGAGRGYLTQMLADCYGIKRVFLVERKSYKLKADRSLRQKESLILERLRIDIEDLNLNA 267

Query: 181 VESLRHNPYLAIGKHLCGPATDLALRCCLTKQPNHAVTERCRDKSKLRGLAIATCCHHLC 240
           VESLRHNPYLAIGKHLCGPATDLALRCCLTKQPN A TE+CRDKSKLRGLAIATCCHHLC
Sbjct: 268 VESLRHNPYLAIGKHLCGPATDLALRCCLTKQPNQADTEQCRDKSKLRGLAIATCCHHLC 327

Query: 241 QWNHYTNKRYLLELGITKEEFLAITWFTSWAVDANHSEDISDVTDSKTFFQSSENESDGM 300
           QW HYTNKRYLLELGITKEEFLAITWFTSWAVDANHSEDISDVTDSKTF QSSENESDGM
Sbjct: 328 QWKHYTNKRYLLELGITKEEFLAITWFTSWAVDANHSEDISDVTDSKTFLQSSENESDGM 387

Query: 301 IGCMVKDIVKSMNPVERAVLGFMCKEIIDMGRLMWLKECGLETQLVKYVPSTISPENHLL 360
           IGC V +IVKSMNPVERAVLGFMCKEIIDMGRLMWLKECGLETQLVKYVPSTISPENHLL
Sbjct: 388 IGCTVDEIVKSMNPVERAVLGFMCKEIIDMGRLMWLKECGLETQLVKYVPSTISPENHLL 447

Query: 361 IAKCSRHICAT 372
           IAKC R+ICAT
Sbjct: 448 IAKC-RNICAT 457

BLAST of CsaV3_7G030430 vs. NCBI nr
Match: TYK10359.1 (tRNA:m(4)X modification enzyme TRM13-like protein isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 723.8 bits (1867), Expect = 7.7e-205
Identity = 355/371 (95.69%), Postives = 362/371 (97.57%), Query Frame = 0

Query: 1   MSSNGPHSGLSDSISSEMKRNIVYGMSGDQFHRLLGKIRALHDLICKDIQDSYKIKEACN 60
           MS+NGP SGLSDSISSEMKRNIVYGMSGDQFHRLLGKIRALHDLICKDIQDSY+IKEACN
Sbjct: 88  MSANGPDSGLSDSISSEMKRNIVYGMSGDQFHRLLGKIRALHDLICKDIQDSYQIKEACN 147

Query: 61  MWIKGEIDRKIPFQEKHVLQQASILGNLDEFGVLSNYDGGEQREGDRSYGNDANVVPAVV 120
           MWIKGEIDRKIPFQEKHVLQQASILGNLDEFGVL NYDGG+QREG+RSYGNDANVVPAVV
Sbjct: 148 MWIKGEIDRKIPFQEKHVLQQASILGNLDEFGVLRNYDGGQQREGNRSYGNDANVVPAVV 207

Query: 121 EFGAGRGYLTQMLADCYGIKRVFLVERKSYKLKADRSLRQKESLILERLRIDIEDLNLNA 180
           EFGAGRGYLTQMLADCYGIKRVFLVERKSYKLKADRSLRQKESLILERLRIDIEDLNLNA
Sbjct: 208 EFGAGRGYLTQMLADCYGIKRVFLVERKSYKLKADRSLRQKESLILERLRIDIEDLNLNA 267

Query: 181 VESLRHNPYLAIGKHLCGPATDLALRCCLTKQPNHAVTERCRDKSKLRGLAIATCCHHLC 240
           VESLRHNPYLAIGKHLCGPATDLALRCCLTKQPN A TE+CRDKSKLRGLAIATCCHHLC
Sbjct: 268 VESLRHNPYLAIGKHLCGPATDLALRCCLTKQPNQADTEQCRDKSKLRGLAIATCCHHLC 327

Query: 241 QWNHYTNKRYLLELGITKEEFLAITWFTSWAVDANHSEDISDVTDSKTFFQSSENESDGM 300
           QW HYTNKRYLLELGITKEEFLAITWFTSWAVDANHSEDISDVTDSKTF QSSENESDGM
Sbjct: 328 QWKHYTNKRYLLELGITKEEFLAITWFTSWAVDANHSEDISDVTDSKTFLQSSENESDGM 387

Query: 301 IGCMVKDIVKSMNPVERAVLGFMCKEIIDMGRLMWLKECGLETQLVKYVPSTISPENHLL 360
           IGC V +IVKSMNPVERAVLGFMCKEIIDMGRLMWLKECGLETQLVKYVPSTISPENHLL
Sbjct: 388 IGCTVDEIVKSMNPVERAVLGFMCKEIIDMGRLMWLKECGLETQLVKYVPSTISPENHLL 447

Query: 361 IAKCSRHICAT 372
           IAKC R+ICAT
Sbjct: 448 IAKC-RNICAT 457

BLAST of CsaV3_7G030430 vs. NCBI nr
Match: XP_008461389.1 (PREDICTED: tRNA:m(4)X modification enzyme TRM13 homolog isoform X3 [Cucumis melo])

HSP 1 Score: 723.8 bits (1867), Expect = 7.7e-205
Identity = 355/371 (95.69%), Postives = 362/371 (97.57%), Query Frame = 0

Query: 1   MSSNGPHSGLSDSISSEMKRNIVYGMSGDQFHRLLGKIRALHDLICKDIQDSYKIKEACN 60
           MS+NGP SGLSDSISSEMKRNIVYGMSGDQFHRLLGKIRALHDLICKDIQDSY+IKEACN
Sbjct: 1   MSANGPDSGLSDSISSEMKRNIVYGMSGDQFHRLLGKIRALHDLICKDIQDSYQIKEACN 60

Query: 61  MWIKGEIDRKIPFQEKHVLQQASILGNLDEFGVLSNYDGGEQREGDRSYGNDANVVPAVV 120
           MWIKGEIDRKIPFQEKHVLQQASILGNLDEFGVL NYDGG+QREG+RSYGNDANVVPAVV
Sbjct: 61  MWIKGEIDRKIPFQEKHVLQQASILGNLDEFGVLRNYDGGQQREGNRSYGNDANVVPAVV 120

Query: 121 EFGAGRGYLTQMLADCYGIKRVFLVERKSYKLKADRSLRQKESLILERLRIDIEDLNLNA 180
           EFGAGRGYLTQMLADCYGIKRVFLVERKSYKLKADRSLRQKESLILERLRIDIEDLNLNA
Sbjct: 121 EFGAGRGYLTQMLADCYGIKRVFLVERKSYKLKADRSLRQKESLILERLRIDIEDLNLNA 180

Query: 181 VESLRHNPYLAIGKHLCGPATDLALRCCLTKQPNHAVTERCRDKSKLRGLAIATCCHHLC 240
           VESLRHNPYLAIGKHLCGPATDLALRCCLTKQPN A TE+CRDKSKLRGLAIATCCHHLC
Sbjct: 181 VESLRHNPYLAIGKHLCGPATDLALRCCLTKQPNQADTEQCRDKSKLRGLAIATCCHHLC 240

Query: 241 QWNHYTNKRYLLELGITKEEFLAITWFTSWAVDANHSEDISDVTDSKTFFQSSENESDGM 300
           QW HYTNKRYLLELGITKEEFLAITWFTSWAVDANHSEDISDVTDSKTF QSSENESDGM
Sbjct: 241 QWKHYTNKRYLLELGITKEEFLAITWFTSWAVDANHSEDISDVTDSKTFLQSSENESDGM 300

Query: 301 IGCMVKDIVKSMNPVERAVLGFMCKEIIDMGRLMWLKECGLETQLVKYVPSTISPENHLL 360
           IGC V +IVKSMNPVERAVLGFMCKEIIDMGRLMWLKECGLETQLVKYVPSTISPENHLL
Sbjct: 301 IGCTVDEIVKSMNPVERAVLGFMCKEIIDMGRLMWLKECGLETQLVKYVPSTISPENHLL 360

Query: 361 IAKCSRHICAT 372
           IAKC R+ICAT
Sbjct: 361 IAKC-RNICAT 370

BLAST of CsaV3_7G030430 vs. ExPASy Swiss-Prot
Match: Q10B19 (tRNA:m(4)X modification enzyme TRM13 OS=Oryza sativa subsp. japonica OX=39947 GN=TRM13 PE=1 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 7.8e-35
Identity = 73/153 (47.71%), Postives = 105/153 (68.63%), Query Frame = 0

Query: 1   MSSNGPHSGLSDSISSEMKRNIVYGMSGDQFHRLLGKIRALHDLICKDIQDSYKIKEACN 60
           ++S G   G    ++S  KR +V+ ++ D+   L+ KI+  H      ++DS+ + +AC+
Sbjct: 104 INSGGGEGG--GGVTSAAKRALVHKLTKDELRALIEKIKLAHASAAMAMRDSFLVTDACD 163

Query: 61  MWIKGEIDRKIPFQEKHVLQQASILGNLDEFGVLSNYDGGEQREGDRSYGNDANVVPAVV 120
            W++ ++DRK+P+QEKHV QQASI+GN++ FG+L       Q+ G+ +  N     PAVV
Sbjct: 164 NWMRNQVDRKVPYQEKHVTQQASIIGNMEAFGLL-------QKGGEVAEENGVKNAPAVV 223

Query: 121 EFGAGRGYLTQMLADCYGIKRVFLVERKSYKLK 154
           EFGAGRGYLTQMLADCYGI+ VFLVER+SYKLK
Sbjct: 224 EFGAGRGYLTQMLADCYGIRNVFLVERRSYKLK 247

BLAST of CsaV3_7G030430 vs. ExPASy Swiss-Prot
Match: Q8BYH3 (tRNA:m(4)X modification enzyme TRM13 homolog OS=Mus musculus OX=10090 GN=Trmt13 PE=2 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 3.0e-34
Identity = 111/328 (33.84%), Postives = 155/328 (47.26%), Query Frame = 0

Query: 76  KHVLQQASILGNLDEFGVLSNYDGGEQREGDRSYGNDANVVPAVVEFGAGRGYLTQ---- 135
           KH+ QQASILGN+++  +L     G +R                VEFGAG+G L+     
Sbjct: 162 KHLKQQASILGNIEKLKLL-----GPRR--------------CFVEFGAGKGKLSHWVDI 221

Query: 136 MLADCYGIKRVFLVERKSYKLKADRSLRQKESLILERLRIDIEDLNLNAVESLRHN--PY 195
            L D   +    LVER + + K D   R+K+S + ERL+IDI+ L LN V  LR    P 
Sbjct: 222 ALKDAENV-HFILVERVTTRFKVDGKHRKKDS-VFERLQIDIQHLCLNRVPVLREGRLPV 281

Query: 196 LAIGKHLCGPATDLALRCCL-----------------------TKQPNHAVTERCRDK-- 255
           + IGKHLCG ATD+ALRC +                       T++ ++ + +   +K  
Sbjct: 282 VGIGKHLCGVATDVALRCLVETYAASFEEKDEEPLAKRIKNDKTEKESNTLAKEGSEKDV 341

Query: 256 ----SKLRGLAIATCCHHLCQWNHYTNKRYLLELGITKEEFLAITWFTSWAV-DANHSED 315
               + + G+ IA CCHH C W HY  K Y   LG+   EF      +SWA      S +
Sbjct: 342 PETWTPVAGIVIALCCHHRCDWRHYVGKEYFKALGLGAVEFYYFQRMSSWATCGMRTSLE 401

Query: 316 ISDVTDSKTFFQSSENESDGMIGCMVKD-----IVKSMNPVERAVLGFMCKEIIDMGRLM 363
            SDVT  +   Q  ENE     G  + D     +   +   E+  +G +CK +ID GRL 
Sbjct: 402 GSDVTPERKDAQRDENEEHDDGGDRLTDGNTDSLPGILTVEEKKKIGHLCKLLIDQGRLQ 461

BLAST of CsaV3_7G030430 vs. ExPASy Swiss-Prot
Match: Q9NUP7 (tRNA:m(4)X modification enzyme TRM13 homolog OS=Homo sapiens OX=9606 GN=TRMT13 PE=1 SV=2)

HSP 1 Score: 141.7 bits (356), Expect = 1.6e-32
Identity = 118/402 (29.35%), Postives = 180/402 (44.78%), Query Frame = 0

Query: 7   HSGLSDSISSEMKRNIVYGMSGDQFHRLLGKIR----ALHDLICKDIQDSYKIKEACNMW 66
           ++GL D      +   +  +S +Q  +L+ K+R     L+  +   I     + +A N  
Sbjct: 95  NAGLRDETEIPEQLVPISSLSEEQLEKLIKKLRKASEGLNSTLKDHIMSHPALHDALNDP 154

Query: 67  IKGEIDRKIPFQEKHVLQQASILGNLDEFGVLSNYDGGEQREGDRSYGNDANVVPAVVEF 126
             G+         KH+ QQASILGN++   +L     G +R                VEF
Sbjct: 155 KNGD------SATKHLKQQASILGNIENLKLL-----GPRR--------------CFVEF 214

Query: 127 GAGRGYLTQ----MLADCYGIKRVFLVERKSYKLKADRSLRQKESLILERLRIDIEDLNL 186
           GAG+G L+      L D   +    LVE+ + + K D   R+K S + ERL+IDI+ L L
Sbjct: 215 GAGKGKLSHWVDIALKDAEKV-HFILVEKVTTRFKVDGKHRKKNS-VFERLQIDIQHLCL 274

Query: 187 NAVESLRHN--PYLAIGKHLCGPATDLALRCCL-----------------------TKQP 246
           N +  LR    P + IGKHLCG ATDLALRC +                       T++ 
Sbjct: 275 NKIPVLREEKLPVVGIGKHLCGMATDLALRCLVETYAASFEERNEEPLAKRIKNDKTEKE 334

Query: 247 NHAVTERCRDK------SKLRGLAIATCCHHLCQWNHYTNKRYLLELGITKEEFLAITWF 306
            + + +   +K      + + G+ IA CCHH C W HY  K Y   LG+   EF      
Sbjct: 335 IYTLAKEGNEKNVPEKWNPVAGIVIALCCHHRCDWRHYVGKEYFRALGLGAVEFHYFQRM 394

Query: 307 TSWAVDA--NHSEDISDVTDSKTFFQSSENESDGMIGCMVKD-----IVKSMNPVERAVL 363
           +SWA       S + S+ T  +   Q+ ++E     G  + D     +   ++  E+  +
Sbjct: 395 SSWATCGMRKTSLETSNSTTKRQDNQNDDSEEHDDGGYRITDDGADCLPGLLSVEEKKKI 454

BLAST of CsaV3_7G030430 vs. ExPASy Swiss-Prot
Match: Q6C4F8 (tRNA:m(4)X modification enzyme TRM13 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=TRM13 PE=3 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 9.0e-23
Identity = 91/310 (29.35%), Postives = 147/310 (47.42%), Query Frame = 0

Query: 75  EKHVLQQASILGNLDEFGVLSNYDGGEQREGDRSYGNDANVVPAVVEFGAGRGYLTQML- 134
           +KH LQQAS++ ++++ G+L +                      ++EFGAGR  L++ + 
Sbjct: 189 KKHALQQASLIAHMEKTGLLKSSS-------------------RILEFGAGRAELSRYIN 248

Query: 135 --------ADCYGIKRVFLVERKSYKLKADRSL--------RQKESLILERLRIDIEDLN 194
                    D         ++R   ++K D  L         ++   +++RL++DI+DL 
Sbjct: 249 YAICAERQQDDKTAANYLFIDRAMPRMKMDGKLVKDTKDDFPEQPLPLIKRLKMDIKDLM 308

Query: 195 LNAVESLRHNPY---LAIGKHLCGPATDLALRCCLTKQPNHAVTERCRDKSKLRGLAIAT 254
           L+ V      PY   L + KHLCG ATDL L+C L    N AV  +   +++   L IA 
Sbjct: 309 LDGVPV--EGPYDANLIVSKHLCGCATDLTLQCLL----NSAVFTK---EARPAALVIAL 368

Query: 255 CCHHLCQWNHYTNK--RYLLELGITKEEFLAITWFTSWAVDANHSEDISDVTDSKTFFQS 314
           CC  +C    +      +L E G   + F A+T  TSW +     +    V   +T  ++
Sbjct: 369 CCRQICNVQMFPKAGIEWLQERGFDDDGFAALTRMTSWVLCGERPKK-EPVEGEQT--EA 428

Query: 315 SENESDGMIGCMVKDIVKSMNPVE-RAVLGFMCKEIIDMGRLMWLKECGLETQLVKYVPS 362
           ++NE+   +G        S  P E R V+G  C+ ++D GRL  L++ GL+ +LV+Y   
Sbjct: 429 ADNETPAPVG------HPSGLPAEARKVIGLKCRRLLDQGRLHALRKEGLDARLVQYTEM 461

BLAST of CsaV3_7G030430 vs. ExPASy Swiss-Prot
Match: Q54BD5 (tRNA:m(4)X modification enzyme TRM13 homolog OS=Dictyostelium discoideum OX=44689 GN=trm13 PE=3 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 1.7e-21
Identity = 85/278 (30.58%), Postives = 129/278 (46.40%), Query Frame = 0

Query: 74  QEKHVLQQASILGNLDEFGVLSNYDGGEQREGDRSYGNDANVVPAVVEFGAGRGYLTQML 133
           Q KH+ Q++SI+ NL E   L N D                     +EFGAG G L+  +
Sbjct: 247 QLKHIQQESSII-NLLENSNLYNSDN------------------VYLEFGAGSGKLSNHI 306

Query: 134 ADCYGIKR-VFLVERKSYK--LKADRSLR-QKESLILERLRIDIEDLNLNAVESLRHNPY 193
              +  K    L++R  ++   K DR ++ +K      RL IDI  LNL+ +  L   P+
Sbjct: 307 FMSHEKKSGHILIDRMKFRSLKKVDRLIKNEKGCHHFSRLLIDIRHLNLSNLSILEEKPF 366

Query: 194 LAIGKHLCGPATDLALRCC--LTKQPNHAVTERCRDKSKLRGLAIATCCHHLCQWNHYTN 253
           +   KHLCG ATD  L     L    N  V       +  +G+ IATCCHH+C +N Y+N
Sbjct: 367 VITSKHLCGCATDFTLDSIFNLLNNSNEKV------NNNFKGIGIATCCHHICNFNTYSN 426

Query: 254 KRYLL-ELGITKEEFLAITWFTSWAV----DANHSEDISDVTDSKTFFQSSENESDGMIG 313
           + YL  +L IT  EF  I   +SWA       N+ +D ++V +     +  + E      
Sbjct: 427 QSYLKDQLNITPLEFQLICSISSWATIDENKCNNDDDDNEVEEDDELIKKDKKEKKEFEN 486

Query: 314 CMVKDIVKS--MNPVERAVLGFMCKEIIDMGRLMWLKE 339
            +  +  K    +   +  LG+  K +ID GR +++KE
Sbjct: 487 QLKIEFNKQDVFSIKRKEELGYKAKRLIDYGRYLFIKE 499

BLAST of CsaV3_7G030430 vs. ExPASy TrEMBL
Match: A0A0A0K9L5 (tRNA:m(4)X modification enzyme TRM13 OS=Cucumis sativus OX=3659 GN=Csa_7G419580 PE=3 SV=1)

HSP 1 Score: 761.9 bits (1966), Expect = 1.2e-216
Identity = 371/371 (100.00%), Postives = 371/371 (100.00%), Query Frame = 0

Query: 1   MSSNGPHSGLSDSISSEMKRNIVYGMSGDQFHRLLGKIRALHDLICKDIQDSYKIKEACN 60
           MSSNGPHSGLSDSISSEMKRNIVYGMSGDQFHRLLGKIRALHDLICKDIQDSYKIKEACN
Sbjct: 88  MSSNGPHSGLSDSISSEMKRNIVYGMSGDQFHRLLGKIRALHDLICKDIQDSYKIKEACN 147

Query: 61  MWIKGEIDRKIPFQEKHVLQQASILGNLDEFGVLSNYDGGEQREGDRSYGNDANVVPAVV 120
           MWIKGEIDRKIPFQEKHVLQQASILGNLDEFGVLSNYDGGEQREGDRSYGNDANVVPAVV
Sbjct: 148 MWIKGEIDRKIPFQEKHVLQQASILGNLDEFGVLSNYDGGEQREGDRSYGNDANVVPAVV 207

Query: 121 EFGAGRGYLTQMLADCYGIKRVFLVERKSYKLKADRSLRQKESLILERLRIDIEDLNLNA 180
           EFGAGRGYLTQMLADCYGIKRVFLVERKSYKLKADRSLRQKESLILERLRIDIEDLNLNA
Sbjct: 208 EFGAGRGYLTQMLADCYGIKRVFLVERKSYKLKADRSLRQKESLILERLRIDIEDLNLNA 267

Query: 181 VESLRHNPYLAIGKHLCGPATDLALRCCLTKQPNHAVTERCRDKSKLRGLAIATCCHHLC 240
           VESLRHNPYLAIGKHLCGPATDLALRCCLTKQPNHAVTERCRDKSKLRGLAIATCCHHLC
Sbjct: 268 VESLRHNPYLAIGKHLCGPATDLALRCCLTKQPNHAVTERCRDKSKLRGLAIATCCHHLC 327

Query: 241 QWNHYTNKRYLLELGITKEEFLAITWFTSWAVDANHSEDISDVTDSKTFFQSSENESDGM 300
           QWNHYTNKRYLLELGITKEEFLAITWFTSWAVDANHSEDISDVTDSKTFFQSSENESDGM
Sbjct: 328 QWNHYTNKRYLLELGITKEEFLAITWFTSWAVDANHSEDISDVTDSKTFFQSSENESDGM 387

Query: 301 IGCMVKDIVKSMNPVERAVLGFMCKEIIDMGRLMWLKECGLETQLVKYVPSTISPENHLL 360
           IGCMVKDIVKSMNPVERAVLGFMCKEIIDMGRLMWLKECGLETQLVKYVPSTISPENHLL
Sbjct: 388 IGCMVKDIVKSMNPVERAVLGFMCKEIIDMGRLMWLKECGLETQLVKYVPSTISPENHLL 447

Query: 361 IAKCSRHICAT 372
           IAKCSRHICAT
Sbjct: 448 IAKCSRHICAT 458

BLAST of CsaV3_7G030430 vs. ExPASy TrEMBL
Match: A0A5D3CJH7 (tRNA:m(4)X modification enzyme TRM13 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00010 PE=3 SV=1)

HSP 1 Score: 723.8 bits (1867), Expect = 3.7e-205
Identity = 355/371 (95.69%), Postives = 362/371 (97.57%), Query Frame = 0

Query: 1   MSSNGPHSGLSDSISSEMKRNIVYGMSGDQFHRLLGKIRALHDLICKDIQDSYKIKEACN 60
           MS+NGP SGLSDSISSEMKRNIVYGMSGDQFHRLLGKIRALHDLICKDIQDSY+IKEACN
Sbjct: 88  MSANGPDSGLSDSISSEMKRNIVYGMSGDQFHRLLGKIRALHDLICKDIQDSYQIKEACN 147

Query: 61  MWIKGEIDRKIPFQEKHVLQQASILGNLDEFGVLSNYDGGEQREGDRSYGNDANVVPAVV 120
           MWIKGEIDRKIPFQEKHVLQQASILGNLDEFGVL NYDGG+QREG+RSYGNDANVVPAVV
Sbjct: 148 MWIKGEIDRKIPFQEKHVLQQASILGNLDEFGVLRNYDGGQQREGNRSYGNDANVVPAVV 207

Query: 121 EFGAGRGYLTQMLADCYGIKRVFLVERKSYKLKADRSLRQKESLILERLRIDIEDLNLNA 180
           EFGAGRGYLTQMLADCYGIKRVFLVERKSYKLKADRSLRQKESLILERLRIDIEDLNLNA
Sbjct: 208 EFGAGRGYLTQMLADCYGIKRVFLVERKSYKLKADRSLRQKESLILERLRIDIEDLNLNA 267

Query: 181 VESLRHNPYLAIGKHLCGPATDLALRCCLTKQPNHAVTERCRDKSKLRGLAIATCCHHLC 240
           VESLRHNPYLAIGKHLCGPATDLALRCCLTKQPN A TE+CRDKSKLRGLAIATCCHHLC
Sbjct: 268 VESLRHNPYLAIGKHLCGPATDLALRCCLTKQPNQADTEQCRDKSKLRGLAIATCCHHLC 327

Query: 241 QWNHYTNKRYLLELGITKEEFLAITWFTSWAVDANHSEDISDVTDSKTFFQSSENESDGM 300
           QW HYTNKRYLLELGITKEEFLAITWFTSWAVDANHSEDISDVTDSKTF QSSENESDGM
Sbjct: 328 QWKHYTNKRYLLELGITKEEFLAITWFTSWAVDANHSEDISDVTDSKTFLQSSENESDGM 387

Query: 301 IGCMVKDIVKSMNPVERAVLGFMCKEIIDMGRLMWLKECGLETQLVKYVPSTISPENHLL 360
           IGC V +IVKSMNPVERAVLGFMCKEIIDMGRLMWLKECGLETQLVKYVPSTISPENHLL
Sbjct: 388 IGCTVDEIVKSMNPVERAVLGFMCKEIIDMGRLMWLKECGLETQLVKYVPSTISPENHLL 447

Query: 361 IAKCSRHICAT 372
           IAKC R+ICAT
Sbjct: 448 IAKC-RNICAT 457

BLAST of CsaV3_7G030430 vs. ExPASy TrEMBL
Match: A0A1S3CFX2 (tRNA:m(4)X modification enzyme TRM13 OS=Cucumis melo OX=3656 GN=LOC103499984 PE=3 SV=1)

HSP 1 Score: 723.8 bits (1867), Expect = 3.7e-205
Identity = 355/371 (95.69%), Postives = 362/371 (97.57%), Query Frame = 0

Query: 1   MSSNGPHSGLSDSISSEMKRNIVYGMSGDQFHRLLGKIRALHDLICKDIQDSYKIKEACN 60
           MS+NGP SGLSDSISSEMKRNIVYGMSGDQFHRLLGKIRALHDLICKDIQDSY+IKEACN
Sbjct: 1   MSANGPDSGLSDSISSEMKRNIVYGMSGDQFHRLLGKIRALHDLICKDIQDSYQIKEACN 60

Query: 61  MWIKGEIDRKIPFQEKHVLQQASILGNLDEFGVLSNYDGGEQREGDRSYGNDANVVPAVV 120
           MWIKGEIDRKIPFQEKHVLQQASILGNLDEFGVL NYDGG+QREG+RSYGNDANVVPAVV
Sbjct: 61  MWIKGEIDRKIPFQEKHVLQQASILGNLDEFGVLRNYDGGQQREGNRSYGNDANVVPAVV 120

Query: 121 EFGAGRGYLTQMLADCYGIKRVFLVERKSYKLKADRSLRQKESLILERLRIDIEDLNLNA 180
           EFGAGRGYLTQMLADCYGIKRVFLVERKSYKLKADRSLRQKESLILERLRIDIEDLNLNA
Sbjct: 121 EFGAGRGYLTQMLADCYGIKRVFLVERKSYKLKADRSLRQKESLILERLRIDIEDLNLNA 180

Query: 181 VESLRHNPYLAIGKHLCGPATDLALRCCLTKQPNHAVTERCRDKSKLRGLAIATCCHHLC 240
           VESLRHNPYLAIGKHLCGPATDLALRCCLTKQPN A TE+CRDKSKLRGLAIATCCHHLC
Sbjct: 181 VESLRHNPYLAIGKHLCGPATDLALRCCLTKQPNQADTEQCRDKSKLRGLAIATCCHHLC 240

Query: 241 QWNHYTNKRYLLELGITKEEFLAITWFTSWAVDANHSEDISDVTDSKTFFQSSENESDGM 300
           QW HYTNKRYLLELGITKEEFLAITWFTSWAVDANHSEDISDVTDSKTF QSSENESDGM
Sbjct: 241 QWKHYTNKRYLLELGITKEEFLAITWFTSWAVDANHSEDISDVTDSKTFLQSSENESDGM 300

Query: 301 IGCMVKDIVKSMNPVERAVLGFMCKEIIDMGRLMWLKECGLETQLVKYVPSTISPENHLL 360
           IGC V +IVKSMNPVERAVLGFMCKEIIDMGRLMWLKECGLETQLVKYVPSTISPENHLL
Sbjct: 301 IGCTVDEIVKSMNPVERAVLGFMCKEIIDMGRLMWLKECGLETQLVKYVPSTISPENHLL 360

Query: 361 IAKCSRHICAT 372
           IAKC R+ICAT
Sbjct: 361 IAKC-RNICAT 370

BLAST of CsaV3_7G030430 vs. ExPASy TrEMBL
Match: A0A1S3CF14 (tRNA:m(4)X modification enzyme TRM13 OS=Cucumis melo OX=3656 GN=LOC103499984 PE=3 SV=1)

HSP 1 Score: 723.8 bits (1867), Expect = 3.7e-205
Identity = 355/371 (95.69%), Postives = 362/371 (97.57%), Query Frame = 0

Query: 1   MSSNGPHSGLSDSISSEMKRNIVYGMSGDQFHRLLGKIRALHDLICKDIQDSYKIKEACN 60
           MS+NGP SGLSDSISSEMKRNIVYGMSGDQFHRLLGKIRALHDLICKDIQDSY+IKEACN
Sbjct: 88  MSANGPDSGLSDSISSEMKRNIVYGMSGDQFHRLLGKIRALHDLICKDIQDSYQIKEACN 147

Query: 61  MWIKGEIDRKIPFQEKHVLQQASILGNLDEFGVLSNYDGGEQREGDRSYGNDANVVPAVV 120
           MWIKGEIDRKIPFQEKHVLQQASILGNLDEFGVL NYDGG+QREG+RSYGNDANVVPAVV
Sbjct: 148 MWIKGEIDRKIPFQEKHVLQQASILGNLDEFGVLRNYDGGQQREGNRSYGNDANVVPAVV 207

Query: 121 EFGAGRGYLTQMLADCYGIKRVFLVERKSYKLKADRSLRQKESLILERLRIDIEDLNLNA 180
           EFGAGRGYLTQMLADCYGIKRVFLVERKSYKLKADRSLRQKESLILERLRIDIEDLNLNA
Sbjct: 208 EFGAGRGYLTQMLADCYGIKRVFLVERKSYKLKADRSLRQKESLILERLRIDIEDLNLNA 267

Query: 181 VESLRHNPYLAIGKHLCGPATDLALRCCLTKQPNHAVTERCRDKSKLRGLAIATCCHHLC 240
           VESLRHNPYLAIGKHLCGPATDLALRCCLTKQPN A TE+CRDKSKLRGLAIATCCHHLC
Sbjct: 268 VESLRHNPYLAIGKHLCGPATDLALRCCLTKQPNQADTEQCRDKSKLRGLAIATCCHHLC 327

Query: 241 QWNHYTNKRYLLELGITKEEFLAITWFTSWAVDANHSEDISDVTDSKTFFQSSENESDGM 300
           QW HYTNKRYLLELGITKEEFLAITWFTSWAVDANHSEDISDVTDSKTF QSSENESDGM
Sbjct: 328 QWKHYTNKRYLLELGITKEEFLAITWFTSWAVDANHSEDISDVTDSKTFLQSSENESDGM 387

Query: 301 IGCMVKDIVKSMNPVERAVLGFMCKEIIDMGRLMWLKECGLETQLVKYVPSTISPENHLL 360
           IGC V +IVKSMNPVERAVLGFMCKEIIDMGRLMWLKECGLETQLVKYVPSTISPENHLL
Sbjct: 388 IGCTVDEIVKSMNPVERAVLGFMCKEIIDMGRLMWLKECGLETQLVKYVPSTISPENHLL 447

Query: 361 IAKCSRHICAT 372
           IAKC R+ICAT
Sbjct: 448 IAKC-RNICAT 457

BLAST of CsaV3_7G030430 vs. ExPASy TrEMBL
Match: A0A5A7UYK4 (tRNA:m(4)X modification enzyme TRM13 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G00280 PE=3 SV=1)

HSP 1 Score: 719.2 bits (1855), Expect = 9.1e-204
Identity = 355/372 (95.43%), Postives = 362/372 (97.31%), Query Frame = 0

Query: 1   MSSNGPHSGLSDSISSEMKRNIVYGMSGDQFHRLLGKIRALHDLICKDIQDSYKIKEACN 60
           MS+NGP SGLSDSISSEMKRNIVYGMSGDQFHRLLGKIRALHDLICKDIQDSY+IKEACN
Sbjct: 88  MSANGPDSGLSDSISSEMKRNIVYGMSGDQFHRLLGKIRALHDLICKDIQDSYQIKEACN 147

Query: 61  MWIKGEID-RKIPFQEKHVLQQASILGNLDEFGVLSNYDGGEQREGDRSYGNDANVVPAV 120
           MWIKGEID RKIPFQEKHVLQQASILGNLDEFGVL NYDGG+QREG+RSYGNDANVVPAV
Sbjct: 148 MWIKGEIDSRKIPFQEKHVLQQASILGNLDEFGVLRNYDGGQQREGNRSYGNDANVVPAV 207

Query: 121 VEFGAGRGYLTQMLADCYGIKRVFLVERKSYKLKADRSLRQKESLILERLRIDIEDLNLN 180
           VEFGAGRGYLTQMLADCYGIKRVFLVERKSYKLKADRSLRQKESLILERLRIDIEDLNLN
Sbjct: 208 VEFGAGRGYLTQMLADCYGIKRVFLVERKSYKLKADRSLRQKESLILERLRIDIEDLNLN 267

Query: 181 AVESLRHNPYLAIGKHLCGPATDLALRCCLTKQPNHAVTERCRDKSKLRGLAIATCCHHL 240
           AVESLRHNPYLAIGKHLCGPATDLALRCCLTKQPN A TE+CRDKSKLRGLAIATCCHHL
Sbjct: 268 AVESLRHNPYLAIGKHLCGPATDLALRCCLTKQPNQADTEQCRDKSKLRGLAIATCCHHL 327

Query: 241 CQWNHYTNKRYLLELGITKEEFLAITWFTSWAVDANHSEDISDVTDSKTFFQSSENESDG 300
           CQW HYTNKRYLLELGITKEEFLAITWFTSWAVDANHSEDISDVTDSKTF QSSENESDG
Sbjct: 328 CQWKHYTNKRYLLELGITKEEFLAITWFTSWAVDANHSEDISDVTDSKTFLQSSENESDG 387

Query: 301 MIGCMVKDIVKSMNPVERAVLGFMCKEIIDMGRLMWLKECGLETQLVKYVPSTISPENHL 360
           MIGC V +IVKSMNPVERAVLGFMCKEIIDMGRLMWLKECGLETQLVKYVPSTISPENHL
Sbjct: 388 MIGCTVDEIVKSMNPVERAVLGFMCKEIIDMGRLMWLKECGLETQLVKYVPSTISPENHL 447

Query: 361 LIAKCSRHICAT 372
           LIAKC R+ICAT
Sbjct: 448 LIAKC-RNICAT 458

BLAST of CsaV3_7G030430 vs. TAIR 10
Match: AT4G01880.1 (methyltransferases )

HSP 1 Score: 431.0 bits (1107), Expect = 9.6e-121
Identity = 219/359 (61.00%), Postives = 267/359 (74.37%), Query Frame = 0

Query: 9   GLSDSISSEMKRNIVYGMSGDQFHRLLGKIRALHDLICKDIQDSYKIKEACNMWIKGEID 68
           G    ++SEMKRN++Y MS  +FH+L+ KI  +H  ICKDI+DSY   EACN+W   EID
Sbjct: 96  GSCSLVTSEMKRNLLYSMSVSKFHQLIKKIEDVHGGICKDIEDSYLSPEACNIWFNKEID 155

Query: 69  RKIPFQEKHVLQQASILGNLDEFGVLSNYD-GGEQREGDRSYGNDANVVPAVVEFGAGRG 128
           RK+PFQEKHVLQQ SILGNL+E GVL   +   E  E D    +D N  PAVVEFGAGRG
Sbjct: 156 RKLPFQEKHVLQQGSILGNLEEIGVLKRCNINVECCESDLEEKDD-NTAPAVVEFGAGRG 215

Query: 129 YLTQMLADCYGIKRVFLVERKSYKLKADRSLRQKESLILERLRIDIEDLNLNAVESLRHN 188
           YLTQMLADCYGIK+V+LVERKSYKLKADRSLRQKESL+LER+RIDIEDLNLNAVESL   
Sbjct: 216 YLTQMLADCYGIKKVYLVERKSYKLKADRSLRQKESLVLERMRIDIEDLNLNAVESLHGV 275

Query: 189 PYLAIGKHLCGPATDLALRCCLTKQPNHAVTERCRDKSKLRGLAIATCCHHLCQWNHYTN 248
           PY+A+GKHLCGPATDL+LRCCL++Q          +   LRGLAIATCCHHL QW  Y N
Sbjct: 276 PYVAVGKHLCGPATDLSLRCCLSRQDG--------ESPVLRGLAIATCCHHLSQWKSYIN 335

Query: 249 KRYLLELGITKEEFLAITWFTSWAVDANHSEDISDVTDSKTFFQSSENESDGMIG----C 308
           K Y+L LGI+K+EF  +T FTSWAVD +H   +  V D      +++ E +G +      
Sbjct: 336 KEYILSLGISKDEFHIMTSFTSWAVDDDHGSKLPGVDDIDLLDSNAKEEEEGEVRDASLI 395

Query: 309 MVKDIVKSMNPVERAVLGFMCKEIIDMGRLMWLKECGLETQLVKYVPSTISPENHLLIA 363
            V+++VK M P+ERAVLGF CK+IID GR+ W+K+ GL ++LVKY+P++ISPEN LLIA
Sbjct: 396 SVEEVVKKMKPMERAVLGFKCKQIIDAGRMKWVKKHGLNSKLVKYIPASISPENTLLIA 445

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_011659414.12.5e-216100.00tRNA:m(4)X modification enzyme TRM13 homolog isoform X2 [Cucumis sativus] >KAE86... [more]
XP_004135966.12.5e-216100.00tRNA:m(4)X modification enzyme TRM13 homolog isoform X1 [Cucumis sativus][more]
XP_008461388.17.7e-20595.69PREDICTED: tRNA:m(4)X modification enzyme TRM13 homolog isoform X1 [Cucumis melo... [more]
TYK10359.17.7e-20595.69tRNA:m(4)X modification enzyme TRM13-like protein isoform X1 [Cucumis melo var. ... [more]
XP_008461389.17.7e-20595.69PREDICTED: tRNA:m(4)X modification enzyme TRM13 homolog isoform X3 [Cucumis melo... [more]
Match NameE-valueIdentityDescription
Q10B197.8e-3547.71tRNA:m(4)X modification enzyme TRM13 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Q8BYH33.0e-3433.84tRNA:m(4)X modification enzyme TRM13 homolog OS=Mus musculus OX=10090 GN=Trmt13 ... [more]
Q9NUP71.6e-3229.35tRNA:m(4)X modification enzyme TRM13 homolog OS=Homo sapiens OX=9606 GN=TRMT13 P... [more]
Q6C4F89.0e-2329.35tRNA:m(4)X modification enzyme TRM13 OS=Yarrowia lipolytica (strain CLIB 122 / E... [more]
Q54BD51.7e-2130.58tRNA:m(4)X modification enzyme TRM13 homolog OS=Dictyostelium discoideum OX=4468... [more]
Match NameE-valueIdentityDescription
A0A0A0K9L51.2e-216100.00tRNA:m(4)X modification enzyme TRM13 OS=Cucumis sativus OX=3659 GN=Csa_7G419580 ... [more]
A0A5D3CJH73.7e-20595.69tRNA:m(4)X modification enzyme TRM13 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3CFX23.7e-20595.69tRNA:m(4)X modification enzyme TRM13 OS=Cucumis melo OX=3656 GN=LOC103499984 PE=... [more]
A0A1S3CF143.7e-20595.69tRNA:m(4)X modification enzyme TRM13 OS=Cucumis melo OX=3656 GN=LOC103499984 PE=... [more]
A0A5A7UYK49.1e-20495.43tRNA:m(4)X modification enzyme TRM13 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
Match NameE-valueIdentityDescription
AT4G01880.19.6e-12161.00methyltransferases [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007871Methyltransferase TRM13PFAMPF05206TRM13coord: 80..363
e-value: 4.1E-86
score: 288.8
IPR039044tRNA:m(4)X modification enzyme Trm13PANTHERPTHR12998UNCHARACTERIZEDcoord: 12..365
NoneNo IPR availablePANTHERPTHR12998:SF0TRNA:M(4)X MODIFICATION ENZYME TRM13 HOMOLOGcoord: 12..365
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 119..182

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_7G030430.1CsaV3_7G030430.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030488 tRNA methylation
biological_process GO:0008033 tRNA processing
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0106050 tRNA 2'-O-methyltransferase activity