CsaV3_6G051460 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_6G051460
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Description4a-hydroxytetrahydrobiopterin dehydratase
Locationchr6: 29937781 .. 29940656 (-)
RNA-Seq ExpressionCsaV3_6G051460
SyntenyCsaV3_6G051460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATAATGGAAAGGTAAATAAACTACGGGTATGGCTATGAATTCCAAATTTTGACGGTCATCGGCGCACGTGTTAAGTTGGAGGTTGGGGTTGGTGTGAGACATTCGCGTAACACTTTTTTATCGGCAAAAATCGCCAATGTCGACTCTGTTTCCGTGTTCCCAATCTCCGTTGCGCTTGATTTTCAAAGCTAAGGTTGTTTCACAATCATCTAACCCGATTCACTGTTGATTCTTTTCACTAATTTCACATTTTGCAGGTCCCGTTTCCGTCGCTGTTTCACACTATCTCTCGCTCTCATGGACGGTGATTCGCTTCTCCCTATTCAAATGTCTTTAGGTTTACCATTTGATGACGTGCTGATGATGTTTGAATCGAATCTCTATGTTATTGGAATTCTTCGCGTTAAAATTTCATTGCATGCTAGGTGTTTGATTATATGTCTGACTCGCTCATTTGATTGCTTTATTAATCTGTAAACGAGCGATAGCGGTTGAGAAACGCAGTGTTTCGCATGTGGTTAATCTGCGATTCTTTTGTTTGGCTTACGTAATTAACAGTCCGGACGTCCGATCATCAAAGATTAATAAAAAAAAAAAGGTGTAGAAAGGATGATTATGTGTGTTGGTTGATGGATTGAGTGTTCGGAAAACATGTGGCCTGATAATTTCAAGTGGAATGCATATGGCCTATGACCTTAGTTGCTTTCATATTATCACATTAGGAATCGTATTTTTTATTTATTCTGGACACTGCACACATGCGTTTGCCTATACCTGATTATTGAAGCCTCATGTGTTGGGTTGAACCCGCATGTGAAGCCATTGTGGACAACCTTCTTTGAGCGGATAGTTACTGTGATGTTTGTATGAATAGCAATACAGTATTAAAGTTTCATCACTATGGGCTTCGTTTTAGTTTCAGACAAAGGTAATTTGTGTATGTGTACAGTCCAATGTCTCGAATTACTGGGATATGTTGGGAGCATGTGGGCTTACCATCAACTAGGAAACCTCTTTCTGGATTTAGAATGCTTTGTACTGGCGAAGGTGCGTGTTGAGCAGCTTTTGCAAAGTGTTAACGTGATTTTTTATTTATTTATGCATGAGATTTTCCTAGTTGATGACTAATCCAACCAGTGCATCCTATCGTCAATCTCTTTTTGTGGCAGCCTTCTCACTGTCCCATGTCCAAAACACACTGCTTGTTTTGCATATGCATCATATTTGAAATTTGTGTACGTAGTTTGCCTATCTGACATAATTTACCATTTTGTATGTGTCAGATTTGACCACTAAAAAATGTGTTCCTTGCAATTCAAATGATTTGCGACCAATGACTGAGGATGCAGCAAAACGTTTGATTAGAGAGGTATGTGGTGTCCATCTCTGTTTGTTAAAACTATCATCTTATACATTGCATTGCATTGAATTTCATTTCATTTATTCATTTCTCTTTATTGTCTATAGCTGGTTGACTGGAACCTTGTAAATGAAGATAGTAAGCTTAAGTTGAGGCGATCGCTGAAGGTGAAGAGTTTTCTTAAAGGAATGGATTTGTTTCAACTTATAGCAGAAGTTGCAGAGGCAGAAGGTATCATGCTTTTCATCTACCAAATATATCATGCACTCTGTTTCTGTTTTCTTGAATCAGAATTTTACTGAGCTATAGATCTGATTTCTAGAATTCAATGCAGGCCATCACCCTGATCTTCATCTAGTTGGATGGAACAATGTAACAATTGAAATCTGGACACATGCAGTAGGTAAGACTTGTTTTTTATGTAGCATTAAATAAAATTCCCTAAAATTTAAACGTAAAACTCAATTTTCTTATACAAGTATGAGTTTTGTTCTTTTCACTCTGTAATTTTCAATGGCCTATTTTAAACTGTGTAAAAGACTTATTCAACATAAAGAGATCATCTTTCTCAACATTTCAAACTAATTCCTGTTCGTTGAAACAATATTATTATTTACGAGTCTTATATTTGTAATATGCTGACCTGATCCTTGAAAACTATGTGGACATTTGGCAAAACATTTCCGGACAGTGAGTATAAAAAGTTTTATAAAAAAAAATTCAAATTCAGGATGTTATACGTTCCTTCCCGTCTTCTTTACCCCTAAACAAATCGCAAAAGAGGGTGAAGGCTCAATGGGACCAATATGTTACGAGGACGTCGGGAACACTTCTATAACTATTTAGCTAACAAGTTGTATTGAAGTATAACAATGTATTTTATATAATTTTTCACATCTATGTTGTAATATAAGCTATGAAAACCTTTTTTCTTGTCTTCCATTAGATTGTATATTCATCTACCTTGTTTATAGATTGGTTTCTTTCATACCAAACTAATTTCCCGACTTATTTACCCCCCAAGTTGTTCAAGTTTGTTTTTACTTAGATGTGTGTGATATCGGCTGACTTAATTAGCTTTTGGGCAGGTGGACTGACTGAAAACGACTTCATTCTCGCTTCCAAGATCAGCAAGCTTGATGTTCACCACCTACTGAGCCGGAAAGTTTCCATTAGCAGTCAACATGATTGTTGAACAGGAACCAAAGCCTTCAAACATTACTCCAATGTTCTTGTTCATTTTTATGCATTTTTTCTTAAGTCAGATTCCCAGGTTGCCTCTTCATACATGACGCGTTGATTCAGTTAAGTTTTGCAGTTTGAATGATAGTTGTTACTATCTTTGGGGGGGTTTAATTAAGAACTGTGATTTCTTTTTCCCTATATTGGTGTGGTTTTGTGAGCTTAAACTCTGTAGGTGAAAGGTTGTGATAATATCATTTTAGTTTTTGTTCAAATGTCCAAGTTTTTTACAAGTTTTTGGTAATTTACTTTTTTTTTTTTGTTTTTTTTTT

mRNA sequence

ATGTCGACTCTGTTTCCGTGTTCCCAATCTCCGTTGCGCTTGATTTTCAAAGCTAAGGTCCCGTTTCCGTCGCTGTTTCACACTATCTCTCGCTCTCATGGACGTCCAATGTCTCGAATTACTGGGATATGTTGGGAGCATGTGGGCTTACCATCAACTAGGAAACCTCTTTCTGGATTTAGAATGCTTTGTACTGGCGAAGATTTGACCACTAAAAAATGTGTTCCTTGCAATTCAAATGATTTGCGACCAATGACTGAGGATGCAGCAAAACGTTTGATTAGAGAGCTGGTTGACTGGAACCTTGTAAATGAAGATAGTAAGCTTAAGTTGAGGCGATCGCTGAAGGTGAAGAGTTTTCTTAAAGGAATGGATTTGTTTCAACTTATAGCAGAAGTTGCAGAGGCAGAAGAATTCAATGCAGGCCATCACCCTGATCTTCATCTAGTTGGATGGAACAATGTAACAATTGAAATCTGGACACATGCAGTAGGTGGACTGACTGAAAACGACTTCATTCTCGCTTCCAAGATCAGCAAGCTTGATGTTCACCACCTACTGAGCCGGAAAGTTTCCATTAGCAGTCAACATGATTGTTGA

Coding sequence (CDS)

ATGTCGACTCTGTTTCCGTGTTCCCAATCTCCGTTGCGCTTGATTTTCAAAGCTAAGGTCCCGTTTCCGTCGCTGTTTCACACTATCTCTCGCTCTCATGGACGTCCAATGTCTCGAATTACTGGGATATGTTGGGAGCATGTGGGCTTACCATCAACTAGGAAACCTCTTTCTGGATTTAGAATGCTTTGTACTGGCGAAGATTTGACCACTAAAAAATGTGTTCCTTGCAATTCAAATGATTTGCGACCAATGACTGAGGATGCAGCAAAACGTTTGATTAGAGAGCTGGTTGACTGGAACCTTGTAAATGAAGATAGTAAGCTTAAGTTGAGGCGATCGCTGAAGGTGAAGAGTTTTCTTAAAGGAATGGATTTGTTTCAACTTATAGCAGAAGTTGCAGAGGCAGAAGAATTCAATGCAGGCCATCACCCTGATCTTCATCTAGTTGGATGGAACAATGTAACAATTGAAATCTGGACACATGCAGTAGGTGGACTGACTGAAAACGACTTCATTCTCGCTTCCAAGATCAGCAAGCTTGATGTTCACCACCTACTGAGCCGGAAAGTTTCCATTAGCAGTCAACATGATTGTTGA

Protein sequence

MSTLFPCSQSPLRLIFKAKVPFPSLFHTISRSHGRPMSRITGICWEHVGLPSTRKPLSGFRMLCTGEDLTTKKCVPCNSNDLRPMTEDAAKRLIRELVDWNLVNEDSKLKLRRSLKVKSFLKGMDLFQLIAEVAEAEEFNAGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKISKLDVHHLLSRKVSISSQHDC*
Homology
BLAST of CsaV3_6G051460 vs. NCBI nr
Match: XP_031743552.1 (pterin-4-alpha-carbinolamine dehydratase 2, mitochondrial isoform X1 [Cucumis sativus] >KAE8647740.1 hypothetical protein Csa_003969 [Cucumis sativus])

HSP 1 Score: 412.9 bits (1060), Expect = 1.6e-111
Identity = 199/199 (100.00%), Postives = 199/199 (100.00%), Query Frame = 0

Query: 1   MSTLFPCSQSPLRLIFKAKVPFPSLFHTISRSHGRPMSRITGICWEHVGLPSTRKPLSGF 60
           MSTLFPCSQSPLRLIFKAKVPFPSLFHTISRSHGRPMSRITGICWEHVGLPSTRKPLSGF
Sbjct: 1   MSTLFPCSQSPLRLIFKAKVPFPSLFHTISRSHGRPMSRITGICWEHVGLPSTRKPLSGF 60

Query: 61  RMLCTGEDLTTKKCVPCNSNDLRPMTEDAAKRLIRELVDWNLVNEDSKLKLRRSLKVKSF 120
           RMLCTGEDLTTKKCVPCNSNDLRPMTEDAAKRLIRELVDWNLVNEDSKLKLRRSLKVKSF
Sbjct: 61  RMLCTGEDLTTKKCVPCNSNDLRPMTEDAAKRLIRELVDWNLVNEDSKLKLRRSLKVKSF 120

Query: 121 LKGMDLFQLIAEVAEAEEFNAGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKISK 180
           LKGMDLFQLIAEVAEAEEFNAGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKISK
Sbjct: 121 LKGMDLFQLIAEVAEAEEFNAGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKISK 180

Query: 181 LDVHHLLSRKVSISSQHDC 200
           LDVHHLLSRKVSISSQHDC
Sbjct: 181 LDVHHLLSRKVSISSQHDC 199

BLAST of CsaV3_6G051460 vs. NCBI nr
Match: XP_004134631.1 (pterin-4-alpha-carbinolamine dehydratase 2, mitochondrial isoform X2 [Cucumis sativus])

HSP 1 Score: 398.7 bits (1023), Expect = 3.0e-107
Identity = 195/199 (97.99%), Postives = 195/199 (97.99%), Query Frame = 0

Query: 1   MSTLFPCSQSPLRLIFKAKVPFPSLFHTISRSHGRPMSRITGICWEHVGLPSTRKPLSGF 60
           MSTLFPCSQSPLRLIFKAKVPFPSLFHTISRSHGRPMSRITGICWEHVGLPSTRKPLSGF
Sbjct: 1   MSTLFPCSQSPLRLIFKAKVPFPSLFHTISRSHGRPMSRITGICWEHVGLPSTRKPLSGF 60

Query: 61  RMLCTGEDLTTKKCVPCNSNDLRPMTEDAAKRLIRELVDWNLVNEDSKLKLRRSLKVKSF 120
           RMLCTGEDLTTKKCVPCNSNDLRPMTEDAAKRLIRELVDWNLVNEDSKLKLRRSLKVKSF
Sbjct: 61  RMLCTGEDLTTKKCVPCNSNDLRPMTEDAAKRLIRELVDWNLVNEDSKLKLRRSLKVKSF 120

Query: 121 LKGMDLFQLIAEVAEAEEFNAGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKISK 180
           LKGMDLFQLIAEVAEAE    GHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKISK
Sbjct: 121 LKGMDLFQLIAEVAEAE----GHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKISK 180

Query: 181 LDVHHLLSRKVSISSQHDC 200
           LDVHHLLSRKVSISSQHDC
Sbjct: 181 LDVHHLLSRKVSISSQHDC 195

BLAST of CsaV3_6G051460 vs. NCBI nr
Match: XP_008439669.1 (PREDICTED: putative pterin-4-alpha-carbinolamine dehydratase isoform X1 [Cucumis melo] >KAA0052567.1 putative pterin-4-alpha-carbinolamine dehydratase isoform X1 [Cucumis melo var. makuwa] >TYK13258.1 putative pterin-4-alpha-carbinolamine dehydratase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 374.0 bits (959), Expect = 8.0e-100
Identity = 187/199 (93.97%), Postives = 189/199 (94.97%), Query Frame = 0

Query: 1   MSTLFPCSQSPLRLIFKAKVPFPSLFHTISRSHGRPMSRITGICWEHVGLPSTRKPLSGF 60
           M+TLFP SQS LRLIFKAKVPFPSLFHTI RSHGRPMSRITGIC EHVGLPSTRKPLSGF
Sbjct: 1   MTTLFPFSQSHLRLIFKAKVPFPSLFHTIPRSHGRPMSRITGICMEHVGLPSTRKPLSGF 60

Query: 61  RMLCTGEDLTTKKCVPCNSNDLRPMTEDAAKRLIRELVDWNLVNEDSKLKLRRSLKVKSF 120
           RMLCTGEDLTTKKCVPCNSNDLRPMTEDAAKRLIRELVDWNLVNEDSKLKL RSLKVKSF
Sbjct: 61  RMLCTGEDLTTKKCVPCNSNDLRPMTEDAAKRLIRELVDWNLVNEDSKLKLSRSLKVKSF 120

Query: 121 LKGMDLFQLIAEVAEAEEFNAGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKISK 180
           LKGM+LFQLIAEVAEAE    GHHPDLHLVGWNNVTIEI THAVGGLTENDFILASKISK
Sbjct: 121 LKGMELFQLIAEVAEAE----GHHPDLHLVGWNNVTIEISTHAVGGLTENDFILASKISK 180

Query: 181 LDVHHLLSRKVSISSQHDC 200
           LDVHHLLSRKVSISSQHDC
Sbjct: 181 LDVHHLLSRKVSISSQHDC 195

BLAST of CsaV3_6G051460 vs. NCBI nr
Match: XP_038881176.1 (pterin-4-alpha-carbinolamine dehydratase 2, mitochondrial isoform X1 [Benincasa hispida])

HSP 1 Score: 360.1 bits (923), Expect = 1.2e-95
Identity = 179/199 (89.95%), Postives = 182/199 (91.46%), Query Frame = 0

Query: 1   MSTLFPCSQSPLRLIFKAKVPFPSLFHTISRSHGRPMSRITGICWEHVGLPSTRKPLSGF 60
           M+TLF  S SPLRLIFK KV  PSLFHTISR HGRPMSRITGICWEHVGL STRK LSGF
Sbjct: 1   MTTLFVFSHSPLRLIFKPKVSIPSLFHTISRPHGRPMSRITGICWEHVGLSSTRKSLSGF 60

Query: 61  RMLCTGEDLTTKKCVPCNSNDLRPMTEDAAKRLIRELVDWNLVNEDSKLKLRRSLKVKSF 120
           RMLCTGEDLTTKKCVPCNSNDLRPMTEDAAKRLIREL DWNLVNED KLKL RSLKVKSF
Sbjct: 61  RMLCTGEDLTTKKCVPCNSNDLRPMTEDAAKRLIRELSDWNLVNEDGKLKLNRSLKVKSF 120

Query: 121 LKGMDLFQLIAEVAEAEEFNAGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKISK 180
           LKGM+LF+LIAEVAEAE    GHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKISK
Sbjct: 121 LKGMELFKLIAEVAEAE----GHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKISK 180

Query: 181 LDVHHLLSRKVSISSQHDC 200
           LDVH LLSRKVSISSQHDC
Sbjct: 181 LDVHDLLSRKVSISSQHDC 195

BLAST of CsaV3_6G051460 vs. NCBI nr
Match: XP_022979014.1 (uncharacterized protein LOC111478787 isoform X1 [Cucurbita maxima])

HSP 1 Score: 351.7 bits (901), Expect = 4.3e-93
Identity = 170/197 (86.29%), Postives = 178/197 (90.36%), Query Frame = 0

Query: 3   TLFPCSQSPLRLIFKAKVPFPSLFHTISRSHGRPMSRITGICWEHVGLPSTRKPLSGFRM 62
           TLF  S SPLRLIFK KVPFPSLFHTI R HGR +SRITGICWEH+GL STRKPLSGFRM
Sbjct: 2   TLFVFSHSPLRLIFKTKVPFPSLFHTIRRPHGRSISRITGICWEHMGLSSTRKPLSGFRM 61

Query: 63  LCTGEDLTTKKCVPCNSNDLRPMTEDAAKRLIRELVDWNLVNEDSKLKLRRSLKVKSFLK 122
           LCTGEDL+TKKCVPCNS DLRPMTEDAAK LIREL +WNL+NED KLKL RSLKVKSFLK
Sbjct: 62  LCTGEDLSTKKCVPCNSKDLRPMTEDAAKHLIRELAEWNLINEDGKLKLNRSLKVKSFLK 121

Query: 123 GMDLFQLIAEVAEAEEFNAGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKISKLD 182
           GMDLF+LIAEVAEAE    GHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKI+KLD
Sbjct: 122 GMDLFKLIAEVAEAE----GHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKINKLD 181

Query: 183 VHHLLSRKVSISSQHDC 200
           VHHLL RK +ISSQHDC
Sbjct: 182 VHHLLGRKAAISSQHDC 194

BLAST of CsaV3_6G051460 vs. ExPASy Swiss-Prot
Match: Q6QJ72 (Pterin-4-alpha-carbinolamine dehydratase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PDL2 PE=1 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 2.8e-47
Identity = 93/176 (52.84%), Postives = 125/176 (71.02%), Query Frame = 0

Query: 15  IFKAKVPFPSLFHTISRSHGRPMSRITGICWEHVGLPSTRKPLSGFRMLCTGEDLTTKKC 74
           I + +VP  SLF+ + R H R +   + +  + V   +T    SG R  C+  DL+TKKC
Sbjct: 12  ISRTQVPAASLFNNLYRRHKRFVHWTSKMSTDSVRSSTTGGSASGARTFCSLADLSTKKC 71

Query: 75  VPCNSNDLRPMTEDAAKRLIRELVDWNLVNEDSKLKLRRSLKVKSFLKGMDLFQLIAEVA 134
           VPCN+ DLR MTE +A+ L++++  W+L N++  LKL RS +VKSF KG+D FQ +A++A
Sbjct: 72  VPCNAKDLRAMTEQSAQDLLQKVAGWDLANDNDTLKLHRSWRVKSFTKGLDFFQRVADIA 131

Query: 135 EAEEFNAGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKISKLDVHHLLSRK 191
           E+E    GHHPDLHLVGWNNV IEIWTHA+GGLTENDFILA+KI++L V  LL +K
Sbjct: 132 ESE----GHHPDLHLVGWNNVKIEIWTHAIGGLTENDFILAAKINELQVEDLLRKK 183

BLAST of CsaV3_6G051460 vs. ExPASy Swiss-Prot
Match: B3ECB9 (Putative pterin-4-alpha-carbinolamine dehydratase OS=Chlorobium limicola (strain DSM 245 / NBRC 103803 / 6330) OX=290315 GN=Clim_1125 PE=3 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 2.8e-15
Identity = 45/114 (39.47%), Postives = 64/114 (56.14%), Query Frame = 0

Query: 68  DLTTKKCVPCNSNDLRPMTEDAAKRLIRELVDWNLVNEDSKLKLRRSLKVKSFLKGMDLF 127
           +L    CVPC +    P+  D   RL +EL DW +V E+  ++LRR+     F + +   
Sbjct: 3   ELKHMACVPC-AEAGSPLAADEIDRLRQELPDWEVVTEEGMVRLRRTFTFPDFGQALAFT 62

Query: 128 QLIAEVAEAEEFNAGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKISKL 182
             + E+AEAE    GHHP L +  W  VT+E WTH+ GGL  NDFI+A++   L
Sbjct: 63  NRVGELAEAE----GHHPRL-VTEWGRVTVEWWTHSAGGLHRNDFIMAARTGGL 110

BLAST of CsaV3_6G051460 vs. ExPASy Swiss-Prot
Match: Q3B6N5 (Putative pterin-4-alpha-carbinolamine dehydratase OS=Pelodictyon luteolum (strain DSM 273 / 2530) OX=319225 GN=Plut_0106 PE=3 SV=2)

HSP 1 Score: 80.5 bits (197), Expect = 2.4e-14
Identity = 42/114 (36.84%), Postives = 68/114 (59.65%), Query Frame = 0

Query: 68  DLTTKKCVPCNSNDLRPMTEDAAKRLIRELVDWNLVNEDSKLKLRRSLKVKSFLKGMDLF 127
           +L   KCV C S  L P+ E  ++ L++E+ +W +V+ED   +L R+   ++F + M   
Sbjct: 3   ELNKTKCVSC-SEGLPPLAERESEELLKEIPEWVIVSEDGVSRLVRTFTFENFREAMAFA 62

Query: 128 QLIAEVAEAEEFNAGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKISKL 182
             + E+AE+E+    HHP L +  W  V +E WTHAV GL  NDF++A++  +L
Sbjct: 63  GSVGELAESEQ----HHPKL-VTEWGKVRVEWWTHAVHGLHMNDFVMAARTDEL 110

BLAST of CsaV3_6G051460 vs. ExPASy Swiss-Prot
Match: Q9LU63 (Probable pterin-4-alpha-carbinolamine dehydratase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ATP1 PE=1 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 3.1e-14
Identity = 47/123 (38.21%), Postives = 75/123 (60.98%), Query Frame = 0

Query: 69  LTTKKCVPCNSNDLRPMTEDAAKRLIRELVDWNLV-NEDSKLKLRRSLKVKSFLKGMDLF 128
           L+  +C P +S+   P++ D A+ L+ +++ W++V NE   LK+R   KV+ F  G++L 
Sbjct: 99  LSQLQCSPVSSSQ-PPLSGDDARTLLHKVLGWSIVDNEAGGLKIRCMWKVRDFGCGVELI 158

Query: 129 QLIAEVAEAEEFNAGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKISKLDVHHLL 188
             I +VAEA    +GH+P LHL     V  E++T ++GGL+ NDFI+A+KI  +    L 
Sbjct: 159 NRIHKVAEA----SGHYPSLHLESPTQVRAELFTSSIGGLSMNDFIMAAKIDDIKTSDLS 216

Query: 189 SRK 191
            RK
Sbjct: 219 PRK 216

BLAST of CsaV3_6G051460 vs. ExPASy Swiss-Prot
Match: Q5QU19 (Putative pterin-4-alpha-carbinolamine dehydratase OS=Idiomarina loihiensis (strain ATCC BAA-735 / DSM 15497 / L2-TR) OX=283942 GN=IL0724 PE=3 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 4.1e-14
Identity = 39/110 (35.45%), Postives = 66/110 (60.00%), Query Frame = 0

Query: 68  DLTTKKCVPCNSNDLRPMTEDAAKRLIRELVDWNLVNEDSKLKLRRSLKVKSFLKGMDLF 127
           +L   +C  C++ D   ++++  K L+RE+ DW  V  D+ + L+R  K K+F + +   
Sbjct: 3   NLENARCEACHA-DAPQVSDEELKELMREIPDWTPVTNDNVMMLQREFKFKNFKQALAFT 62

Query: 128 QLIAEVAEAEEFNAGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASK 178
             + ++AE E+    HHP+L +  W  VT+  WTHA+ GL +NDFI+A+K
Sbjct: 63  NRVGDLAEEEK----HHPEL-VTEWGKVTVTWWTHAINGLHKNDFIMAAK 106

BLAST of CsaV3_6G051460 vs. ExPASy TrEMBL
Match: A0A0A0KIH0 (4a-hydroxytetrahydrobiopterin dehydratase OS=Cucumis sativus OX=3659 GN=Csa_6G522130 PE=3 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 1.5e-107
Identity = 195/199 (97.99%), Postives = 195/199 (97.99%), Query Frame = 0

Query: 1   MSTLFPCSQSPLRLIFKAKVPFPSLFHTISRSHGRPMSRITGICWEHVGLPSTRKPLSGF 60
           MSTLFPCSQSPLRLIFKAKVPFPSLFHTISRSHGRPMSRITGICWEHVGLPSTRKPLSGF
Sbjct: 1   MSTLFPCSQSPLRLIFKAKVPFPSLFHTISRSHGRPMSRITGICWEHVGLPSTRKPLSGF 60

Query: 61  RMLCTGEDLTTKKCVPCNSNDLRPMTEDAAKRLIRELVDWNLVNEDSKLKLRRSLKVKSF 120
           RMLCTGEDLTTKKCVPCNSNDLRPMTEDAAKRLIRELVDWNLVNEDSKLKLRRSLKVKSF
Sbjct: 61  RMLCTGEDLTTKKCVPCNSNDLRPMTEDAAKRLIRELVDWNLVNEDSKLKLRRSLKVKSF 120

Query: 121 LKGMDLFQLIAEVAEAEEFNAGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKISK 180
           LKGMDLFQLIAEVAEAE    GHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKISK
Sbjct: 121 LKGMDLFQLIAEVAEAE----GHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKISK 180

Query: 181 LDVHHLLSRKVSISSQHDC 200
           LDVHHLLSRKVSISSQHDC
Sbjct: 181 LDVHHLLSRKVSISSQHDC 195

BLAST of CsaV3_6G051460 vs. ExPASy TrEMBL
Match: A0A5D3CQ07 (4a-hydroxytetrahydrobiopterin dehydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008760 PE=3 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 3.9e-100
Identity = 187/199 (93.97%), Postives = 189/199 (94.97%), Query Frame = 0

Query: 1   MSTLFPCSQSPLRLIFKAKVPFPSLFHTISRSHGRPMSRITGICWEHVGLPSTRKPLSGF 60
           M+TLFP SQS LRLIFKAKVPFPSLFHTI RSHGRPMSRITGIC EHVGLPSTRKPLSGF
Sbjct: 1   MTTLFPFSQSHLRLIFKAKVPFPSLFHTIPRSHGRPMSRITGICMEHVGLPSTRKPLSGF 60

Query: 61  RMLCTGEDLTTKKCVPCNSNDLRPMTEDAAKRLIRELVDWNLVNEDSKLKLRRSLKVKSF 120
           RMLCTGEDLTTKKCVPCNSNDLRPMTEDAAKRLIRELVDWNLVNEDSKLKL RSLKVKSF
Sbjct: 61  RMLCTGEDLTTKKCVPCNSNDLRPMTEDAAKRLIRELVDWNLVNEDSKLKLSRSLKVKSF 120

Query: 121 LKGMDLFQLIAEVAEAEEFNAGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKISK 180
           LKGM+LFQLIAEVAEAE    GHHPDLHLVGWNNVTIEI THAVGGLTENDFILASKISK
Sbjct: 121 LKGMELFQLIAEVAEAE----GHHPDLHLVGWNNVTIEISTHAVGGLTENDFILASKISK 180

Query: 181 LDVHHLLSRKVSISSQHDC 200
           LDVHHLLSRKVSISSQHDC
Sbjct: 181 LDVHHLLSRKVSISSQHDC 195

BLAST of CsaV3_6G051460 vs. ExPASy TrEMBL
Match: A0A1S3AZX5 (4a-hydroxytetrahydrobiopterin dehydratase OS=Cucumis melo OX=3656 GN=LOC103484393 PE=3 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 3.9e-100
Identity = 187/199 (93.97%), Postives = 189/199 (94.97%), Query Frame = 0

Query: 1   MSTLFPCSQSPLRLIFKAKVPFPSLFHTISRSHGRPMSRITGICWEHVGLPSTRKPLSGF 60
           M+TLFP SQS LRLIFKAKVPFPSLFHTI RSHGRPMSRITGIC EHVGLPSTRKPLSGF
Sbjct: 1   MTTLFPFSQSHLRLIFKAKVPFPSLFHTIPRSHGRPMSRITGICMEHVGLPSTRKPLSGF 60

Query: 61  RMLCTGEDLTTKKCVPCNSNDLRPMTEDAAKRLIRELVDWNLVNEDSKLKLRRSLKVKSF 120
           RMLCTGEDLTTKKCVPCNSNDLRPMTEDAAKRLIRELVDWNLVNEDSKLKL RSLKVKSF
Sbjct: 61  RMLCTGEDLTTKKCVPCNSNDLRPMTEDAAKRLIRELVDWNLVNEDSKLKLSRSLKVKSF 120

Query: 121 LKGMDLFQLIAEVAEAEEFNAGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKISK 180
           LKGM+LFQLIAEVAEAE    GHHPDLHLVGWNNVTIEI THAVGGLTENDFILASKISK
Sbjct: 121 LKGMELFQLIAEVAEAE----GHHPDLHLVGWNNVTIEISTHAVGGLTENDFILASKISK 180

Query: 181 LDVHHLLSRKVSISSQHDC 200
           LDVHHLLSRKVSISSQHDC
Sbjct: 181 LDVHHLLSRKVSISSQHDC 195

BLAST of CsaV3_6G051460 vs. ExPASy TrEMBL
Match: A0A6J1IVJ8 (4a-hydroxytetrahydrobiopterin dehydratase OS=Cucurbita maxima OX=3661 GN=LOC111478787 PE=3 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 2.1e-93
Identity = 170/197 (86.29%), Postives = 178/197 (90.36%), Query Frame = 0

Query: 3   TLFPCSQSPLRLIFKAKVPFPSLFHTISRSHGRPMSRITGICWEHVGLPSTRKPLSGFRM 62
           TLF  S SPLRLIFK KVPFPSLFHTI R HGR +SRITGICWEH+GL STRKPLSGFRM
Sbjct: 2   TLFVFSHSPLRLIFKTKVPFPSLFHTIRRPHGRSISRITGICWEHMGLSSTRKPLSGFRM 61

Query: 63  LCTGEDLTTKKCVPCNSNDLRPMTEDAAKRLIRELVDWNLVNEDSKLKLRRSLKVKSFLK 122
           LCTGEDL+TKKCVPCNS DLRPMTEDAAK LIREL +WNL+NED KLKL RSLKVKSFLK
Sbjct: 62  LCTGEDLSTKKCVPCNSKDLRPMTEDAAKHLIRELAEWNLINEDGKLKLNRSLKVKSFLK 121

Query: 123 GMDLFQLIAEVAEAEEFNAGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKISKLD 182
           GMDLF+LIAEVAEAE    GHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKI+KLD
Sbjct: 122 GMDLFKLIAEVAEAE----GHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKINKLD 181

Query: 183 VHHLLSRKVSISSQHDC 200
           VHHLL RK +ISSQHDC
Sbjct: 182 VHHLLGRKAAISSQHDC 194

BLAST of CsaV3_6G051460 vs. ExPASy TrEMBL
Match: A0A6J1ECR6 (4a-hydroxytetrahydrobiopterin dehydratase OS=Cucurbita moschata OX=3662 GN=LOC111432994 PE=3 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 1.3e-92
Identity = 169/197 (85.79%), Postives = 178/197 (90.36%), Query Frame = 0

Query: 3   TLFPCSQSPLRLIFKAKVPFPSLFHTISRSHGRPMSRITGICWEHVGLPSTRKPLSGFRM 62
           TLF  S SPLRLIFK KVPFPSLFHTI R HGR +SRITGICWEH+GL STRKPLSGFRM
Sbjct: 2   TLFVFSHSPLRLIFKTKVPFPSLFHTIRRPHGRSISRITGICWEHMGLSSTRKPLSGFRM 61

Query: 63  LCTGEDLTTKKCVPCNSNDLRPMTEDAAKRLIRELVDWNLVNEDSKLKLRRSLKVKSFLK 122
           LCTGEDL+TKKCVPCNS DLRPMTEDAAK LIREL +WNL+NED KLKL RSLKVKSFLK
Sbjct: 62  LCTGEDLSTKKCVPCNSKDLRPMTEDAAKHLIRELAEWNLINEDGKLKLNRSLKVKSFLK 121

Query: 123 GMDLFQLIAEVAEAEEFNAGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKISKLD 182
           GMDLF+LIAEVAEAE    GHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKI+KLD
Sbjct: 122 GMDLFKLIAEVAEAE----GHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKINKLD 181

Query: 183 VHHLLSRKVSISSQHDC 200
           VH+LL RK +ISSQHDC
Sbjct: 182 VHNLLGRKAAISSQHDC 194

BLAST of CsaV3_6G051460 vs. TAIR 10
Match: AT1G29810.1 (Transcriptional coactivator/pterin dehydratase )

HSP 1 Score: 189.9 bits (481), Expect = 2.0e-48
Identity = 93/176 (52.84%), Postives = 125/176 (71.02%), Query Frame = 0

Query: 15  IFKAKVPFPSLFHTISRSHGRPMSRITGICWEHVGLPSTRKPLSGFRMLCTGEDLTTKKC 74
           I + +VP  SLF+ + R H R +   + +  + V   +T    SG R  C+  DL+TKKC
Sbjct: 12  ISRTQVPAASLFNNLYRRHKRFVHWTSKMSTDSVRSSTTGGSASGARTFCSLADLSTKKC 71

Query: 75  VPCNSNDLRPMTEDAAKRLIRELVDWNLVNEDSKLKLRRSLKVKSFLKGMDLFQLIAEVA 134
           VPCN+ DLR MTE +A+ L++++  W+L N++  LKL RS +VKSF KG+D FQ +A++A
Sbjct: 72  VPCNAKDLRAMTEQSAQDLLQKVAGWDLANDNDTLKLHRSWRVKSFTKGLDFFQRVADIA 131

Query: 135 EAEEFNAGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKISKLDVHHLLSRK 191
           E+E    GHHPDLHLVGWNNV IEIWTHA+GGLTENDFILA+KI++L V  LL +K
Sbjct: 132 ESE----GHHPDLHLVGWNNVKIEIWTHAIGGLTENDFILAAKINELQVEDLLRKK 183

BLAST of CsaV3_6G051460 vs. TAIR 10
Match: AT5G51110.1 (Transcriptional coactivator/pterin dehydratase )

HSP 1 Score: 80.1 bits (196), Expect = 2.2e-15
Identity = 47/123 (38.21%), Postives = 75/123 (60.98%), Query Frame = 0

Query: 69  LTTKKCVPCNSNDLRPMTEDAAKRLIRELVDWNLV-NEDSKLKLRRSLKVKSFLKGMDLF 128
           L+  +C P +S+   P++ D A+ L+ +++ W++V NE   LK+R   KV+ F  G++L 
Sbjct: 99  LSQLQCSPVSSSQ-PPLSGDDARTLLHKVLGWSIVDNEAGGLKIRCMWKVRDFGCGVELI 158

Query: 129 QLIAEVAEAEEFNAGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKISKLDVHHLL 188
             I +VAEA    +GH+P LHL     V  E++T ++GGL+ NDFI+A+KI  +    L 
Sbjct: 159 NRIHKVAEA----SGHYPSLHLESPTQVRAELFTSSIGGLSMNDFIMAAKIDDIKTSDLS 216

Query: 189 SRK 191
            RK
Sbjct: 219 PRK 216

BLAST of CsaV3_6G051460 vs. TAIR 10
Match: AT5G51110.2 (Transcriptional coactivator/pterin dehydratase )

HSP 1 Score: 80.1 bits (196), Expect = 2.2e-15
Identity = 47/123 (38.21%), Postives = 75/123 (60.98%), Query Frame = 0

Query: 69  LTTKKCVPCNSNDLRPMTEDAAKRLIRELVDWNLV-NEDSKLKLRRSLKVKSFLKGMDLF 128
           L+  +C P +S+   P++ D A+ L+ +++ W++V NE   LK+R   KV+ F  G++L 
Sbjct: 72  LSQLQCSPVSSSQ-PPLSGDDARTLLHKVLGWSIVDNEAGGLKIRCMWKVRDFGCGVELI 131

Query: 129 QLIAEVAEAEEFNAGHHPDLHLVGWNNVTIEIWTHAVGGLTENDFILASKISKLDVHHLL 188
             I +VAEA    +GH+P LHL     V  E++T ++GGL+ NDFI+A+KI  +    L 
Sbjct: 132 NRIHKVAEA----SGHYPSLHLESPTQVRAELFTSSIGGLSMNDFIMAAKIDDIKTSDLS 189

Query: 189 SRK 191
            RK
Sbjct: 192 PRK 189

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_031743552.11.6e-111100.00pterin-4-alpha-carbinolamine dehydratase 2, mitochondrial isoform X1 [Cucumis sa... [more]
XP_004134631.13.0e-10797.99pterin-4-alpha-carbinolamine dehydratase 2, mitochondrial isoform X2 [Cucumis sa... [more]
XP_008439669.18.0e-10093.97PREDICTED: putative pterin-4-alpha-carbinolamine dehydratase isoform X1 [Cucumis... [more]
XP_038881176.11.2e-9589.95pterin-4-alpha-carbinolamine dehydratase 2, mitochondrial isoform X1 [Benincasa ... [more]
XP_022979014.14.3e-9386.29uncharacterized protein LOC111478787 isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q6QJ722.8e-4752.84Pterin-4-alpha-carbinolamine dehydratase 2, mitochondrial OS=Arabidopsis thalian... [more]
B3ECB92.8e-1539.47Putative pterin-4-alpha-carbinolamine dehydratase OS=Chlorobium limicola (strain... [more]
Q3B6N52.4e-1436.84Putative pterin-4-alpha-carbinolamine dehydratase OS=Pelodictyon luteolum (strai... [more]
Q9LU633.1e-1438.21Probable pterin-4-alpha-carbinolamine dehydratase, chloroplastic OS=Arabidopsis ... [more]
Q5QU194.1e-1435.45Putative pterin-4-alpha-carbinolamine dehydratase OS=Idiomarina loihiensis (stra... [more]
Match NameE-valueIdentityDescription
A0A0A0KIH01.5e-10797.994a-hydroxytetrahydrobiopterin dehydratase OS=Cucumis sativus OX=3659 GN=Csa_6G52... [more]
A0A5D3CQ073.9e-10093.974a-hydroxytetrahydrobiopterin dehydratase OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3AZX53.9e-10093.974a-hydroxytetrahydrobiopterin dehydratase OS=Cucumis melo OX=3656 GN=LOC10348439... [more]
A0A6J1IVJ82.1e-9386.294a-hydroxytetrahydrobiopterin dehydratase OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1ECR61.3e-9285.794a-hydroxytetrahydrobiopterin dehydratase OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT1G29810.12.0e-4852.84Transcriptional coactivator/pterin dehydratase [more]
AT5G51110.12.2e-1538.21Transcriptional coactivator/pterin dehydratase [more]
AT5G51110.22.2e-1538.21Transcriptional coactivator/pterin dehydratase [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001533Pterin 4 alpha carbinolamine dehydratasePFAMPF01329Pterin_4acoord: 84..180
e-value: 7.3E-23
score: 80.5
IPR001533Pterin 4 alpha carbinolamine dehydratasePANTHERPTHR12599PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASEcoord: 51..192
IPR036428Pterin 4 alpha carbinolamine dehydratase superfamilyGENE3D3.30.1360.20Transcriptional coactivator/pterin dehydratasecoord: 84..185
e-value: 2.3E-23
score: 83.9
IPR036428Pterin 4 alpha carbinolamine dehydratase superfamilySUPERFAMILY55248PCD-likecoord: 83..181
NoneNo IPR availablePANTHERPTHR12599:SF0PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASEcoord: 51..192
NoneNo IPR availableCDDcd00913PCD_DCoH_subfamily_acoord: 99..181
e-value: 8.67353E-33
score: 110.782

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_6G051460.1CsaV3_6G051460.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006729 tetrahydrobiopterin biosynthetic process
molecular_function GO:0008124 4-alpha-hydroxytetrahydrobiopterin dehydratase activity