Cp4.1LG20g02300 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG20g02300
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionRING-type E3 ubiquitin transferase
LocationCp4.1LG20: 1330562 .. 1332265 (+)
RNA-Seq ExpressionCp4.1LG20g02300
SyntenyCp4.1LG20g02300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAATCCCAATTCGTTGCCACTCAGTTTTGGGTTTTGATTCGGGCGCTCGCTACGGCTCTGGATGTACTTCCATTGAATCGGATTGATACTAGCGATGAAGTGAAGGAATTGGTTGAACTAGTTGTGAAACAAGCACGAATTTCGAAATTTGGACTGGATAAAGATGATGAACTGATGATGAAGCGACTTCAATCCATTTTGCTGCAGTTTGAAAAAGGGATTGAACCGGATTTGACCGCAATTAAACGGGTTCTTAGCTACCTTGAAATTCGAAGATGGAGTGATTGTAATAAAGAGATCAAGTTCTTGGAGGAGGAGATTGATTTTCAATATTCGGATTTGAAAGAACGAGACGTTCAGATTCTGAGCAGTTTGGTGGGTTTTATGAGCTACAGCAGATTAACTCTGTTCGAAGCTCTGGATTTTTGGGATAAAAATCAAACGGATATTAAATGCAATCCCGAGACGCTGAGTTGTTTAAACCCACAAGATTTTCGATGCCCAATTTCTCTTGAGCTCATGATTGATCCTGTTACTGTATCTACTGGACAGACATACGATCGAGCTTCGATTCAGAAATGGCTTTCCGCCGGGAATTTGCTCTGCCCCAAAACAGGGGAGAGGCTCACGAGCTCAGAACTGGTTCCGAATACGAGTCTCAAGAAGTTAATCCATCAATTTTGTGTCGACAATGGGATTTCTTTGGCTAAATTCAACGCCAGATCTCATGATATAACTCGAACTATAATTCCGGGGAGTCCGGCTGCTGCAGAAGCGATGAAATTCTCATCGGAGTATCTTCTCCGGCGACTGGTTTTTGGAACGGATACGGAGAAGAACAAAGCGGCTTATGAGATTCGTTTGTTGGCGAAATCGAACATTTTCAATCGGTCTTGTTTGATTAAAGTGGGTATAATTCCATCATTGTTGAATCTTCTTTCGTCTTTGGACAAATCCACGCAGGAAAACGCCATTGCTGCCGTGTTGAAGCTTTCAAAGCATTCGACTGGGAAGATTTTAGTGGTGGAAAATGGAGGATTGGTGCCTATTCTCTCTGTTTTAAAATCTGGGTTTTGTTTGGAATCTCGTCAATTAGCAGCCGCGACATTGTTCTACCTCTCGTCGGTGAAGGAGTACCGGAAATTGATCGGCGAAACGCCGGACGCCATTACAGGGCTAATCGAGCTCATTAAGGAAGGAACAACTTGTGGGAAAAAAAACGCAGTGGTTGCCATTTTCGGACTCTTACTGTACCCCAAAAACAACAAAACCGTTCTTAATTCCGGCGTCGTTCCGATTCTACTCGACATAATCGCCACCTCCGTCAACGACGAGTTGATTTCCGATTCACTTGCAGTAATAGCAGCGTTGGCGGAGAGTTCAGAAGGAAGCAATACGATTCTTCACGCCTCAGCTCTGCCTCTGCTAATAAGAACTCTGAATTCCGCAACTTCGTCGCCTGGAAAAGAGTATTGCGTTTCGGCGCTGCGTTCTCTGTGCAACCACGGCGGCGAGGAGGTGGTGGCGACGCTGGCCGGAGATCGGTCCATTGCGGGGTCGCTTTATTCAATTGTAACGGAGGGAAGTGCGGCGGCGGGGAAGAAGGCTCGATCGCTGCTGAAGATTCTGCACAAGTTTAGGGAAAACGATGTGGGTGTGAACTCGGCGGTGGATCAAGAGCGCTCTGTTCGTGTTTGGTGA

mRNA sequence

ATGAAATCCCAATTCGTTGCCACTCAGTTTTGGGTTTTGATTCGGGCGCTCGCTACGGCTCTGGATGTACTTCCATTGAATCGGATTGATACTAGCGATGAAGTGAAGGAATTGGTTGAACTAGTTGTGAAACAAGCACGAATTTCGAAATTTGGACTGGATAAAGATGATGAACTGATGATGAAGCGACTTCAATCCATTTTGCTGCAGTTTGAAAAAGGGATTGAACCGGATTTGACCGCAATTAAACGGGTTCTTAGCTACCTTGAAATTCGAAGATGGAGTGATTGTAATAAAGAGATCAAGTTCTTGGAGGAGGAGATTGATTTTCAATATTCGGATTTGAAAGAACGAGACGTTCAGATTCTGAGCAGTTTGGTGGGTTTTATGAGCTACAGCAGATTAACTCTGTTCGAAGCTCTGGATTTTTGGGATAAAAATCAAACGGATATTAAATGCAATCCCGAGACGCTGAGTTGTTTAAACCCACAAGATTTTCGATGCCCAATTTCTCTTGAGCTCATGATTGATCCTGTTACTGTATCTACTGGACAGACATACGATCGAGCTTCGATTCAGAAATGGCTTTCCGCCGGGAATTTGCTCTGCCCCAAAACAGGGGAGAGGCTCACGAGCTCAGAACTGGTTCCGAATACGAGTCTCAAGAAGTTAATCCATCAATTTTGTGTCGACAATGGGATTTCTTTGGCTAAATTCAACGCCAGATCTCATGATATAACTCGAACTATAATTCCGGGGAGTCCGGCTGCTGCAGAAGCGATGAAATTCTCATCGGAGTATCTTCTCCGGCGACTGGTTTTTGGAACGGATACGGAGAAGAACAAAGCGGCTTATGAGATTCGTTTGTTGGCGAAATCGAACATTTTCAATCGGTCTTGTTTGATTAAAGTGGGTATAATTCCATCATTGTTGAATCTTCTTTCGTCTTTGGACAAATCCACGCAGGAAAACGCCATTGCTGCCGTGTTGAAGCTTTCAAAGCATTCGACTGGGAAGATTTTAGTGGTGGAAAATGGAGGATTGGTGCCTATTCTCTCTGTTTTAAAATCTGGGTTTTGTTTGGAATCTCGTCAATTAGCAGCCGCGACATTGTTCTACCTCTCGTCGGTGAAGGAGTACCGGAAATTGATCGGCGAAACGCCGGACGCCATTACAGGGCTAATCGAGCTCATTAAGGAAGGAACAACTTGTGGGAAAAAAAACGCAGTGGTTGCCATTTTCGGACTCTTACTGTACCCCAAAAACAACAAAACCGTTCTTAATTCCGGCGTCGTTCCGATTCTACTCGACATAATCGCCACCTCCGTCAACGACGAGTTGATTTCCGATTCACTTGCAGTAATAGCAGCGTTGGCGGAGAGTTCAGAAGGAAGCAATACGATTCTTCACGCCTCAGCTCTGCCTCTGCTAATAAGAACTCTGAATTCCGCAACTTCGTCGCCTGGAAAAGAGTATTGCGTTTCGGCGCTGCGTTCTCTGTGCAACCACGGCGGCGAGGAGGTGGTGGCGACGCTGGCCGGAGATCGGTCCATTGCGGGGTCGCTTTATTCAATTGTAACGGAGGGAAGTGCGGCGGCGGGGAAGAAGGCTCGATCGCTGCTGAAGATTCTGCACAAGTTTAGGGAAAACGATGTGGGTGTGAACTCGGCGGTGGATCAAGAGCGCTCTGTTCGTGTTTGGTGA

Coding sequence (CDS)

ATGAAATCCCAATTCGTTGCCACTCAGTTTTGGGTTTTGATTCGGGCGCTCGCTACGGCTCTGGATGTACTTCCATTGAATCGGATTGATACTAGCGATGAAGTGAAGGAATTGGTTGAACTAGTTGTGAAACAAGCACGAATTTCGAAATTTGGACTGGATAAAGATGATGAACTGATGATGAAGCGACTTCAATCCATTTTGCTGCAGTTTGAAAAAGGGATTGAACCGGATTTGACCGCAATTAAACGGGTTCTTAGCTACCTTGAAATTCGAAGATGGAGTGATTGTAATAAAGAGATCAAGTTCTTGGAGGAGGAGATTGATTTTCAATATTCGGATTTGAAAGAACGAGACGTTCAGATTCTGAGCAGTTTGGTGGGTTTTATGAGCTACAGCAGATTAACTCTGTTCGAAGCTCTGGATTTTTGGGATAAAAATCAAACGGATATTAAATGCAATCCCGAGACGCTGAGTTGTTTAAACCCACAAGATTTTCGATGCCCAATTTCTCTTGAGCTCATGATTGATCCTGTTACTGTATCTACTGGACAGACATACGATCGAGCTTCGATTCAGAAATGGCTTTCCGCCGGGAATTTGCTCTGCCCCAAAACAGGGGAGAGGCTCACGAGCTCAGAACTGGTTCCGAATACGAGTCTCAAGAAGTTAATCCATCAATTTTGTGTCGACAATGGGATTTCTTTGGCTAAATTCAACGCCAGATCTCATGATATAACTCGAACTATAATTCCGGGGAGTCCGGCTGCTGCAGAAGCGATGAAATTCTCATCGGAGTATCTTCTCCGGCGACTGGTTTTTGGAACGGATACGGAGAAGAACAAAGCGGCTTATGAGATTCGTTTGTTGGCGAAATCGAACATTTTCAATCGGTCTTGTTTGATTAAAGTGGGTATAATTCCATCATTGTTGAATCTTCTTTCGTCTTTGGACAAATCCACGCAGGAAAACGCCATTGCTGCCGTGTTGAAGCTTTCAAAGCATTCGACTGGGAAGATTTTAGTGGTGGAAAATGGAGGATTGGTGCCTATTCTCTCTGTTTTAAAATCTGGGTTTTGTTTGGAATCTCGTCAATTAGCAGCCGCGACATTGTTCTACCTCTCGTCGGTGAAGGAGTACCGGAAATTGATCGGCGAAACGCCGGACGCCATTACAGGGCTAATCGAGCTCATTAAGGAAGGAACAACTTGTGGGAAAAAAAACGCAGTGGTTGCCATTTTCGGACTCTTACTGTACCCCAAAAACAACAAAACCGTTCTTAATTCCGGCGTCGTTCCGATTCTACTCGACATAATCGCCACCTCCGTCAACGACGAGTTGATTTCCGATTCACTTGCAGTAATAGCAGCGTTGGCGGAGAGTTCAGAAGGAAGCAATACGATTCTTCACGCCTCAGCTCTGCCTCTGCTAATAAGAACTCTGAATTCCGCAACTTCGTCGCCTGGAAAAGAGTATTGCGTTTCGGCGCTGCGTTCTCTGTGCAACCACGGCGGCGAGGAGGTGGTGGCGACGCTGGCCGGAGATCGGTCCATTGCGGGGTCGCTTTATTCAATTGTAACGGAGGGAAGTGCGGCGGCGGGGAAGAAGGCTCGATCGCTGCTGAAGATTCTGCACAAGTTTAGGGAAAACGATGTGGGTGTGAACTCGGCGGTGGATCAAGAGCGCTCTGTTCGTGTTTGGTGA

Protein sequence

MKSQFVATQFWVLIRALATALDVLPLNRIDTSDEVKELVELVVKQARISKFGLDKDDELMMKRLQSILLQFEKGIEPDLTAIKRVLSYLEIRRWSDCNKEIKFLEEEIDFQYSDLKERDVQILSSLVGFMSYSRLTLFEALDFWDKNQTDIKCNPETLSCLNPQDFRCPISLELMIDPVTVSTGQTYDRASIQKWLSAGNLLCPKTGERLTSSELVPNTSLKKLIHQFCVDNGISLAKFNARSHDITRTIIPGSPAAAEAMKFSSEYLLRRLVFGTDTEKNKAAYEIRLLAKSNIFNRSCLIKVGIIPSLLNLLSSLDKSTQENAIAAVLKLSKHSTGKILVVENGGLVPILSVLKSGFCLESRQLAAATLFYLSSVKEYRKLIGETPDAITGLIELIKEGTTCGKKNAVVAIFGLLLYPKNNKTVLNSGVVPILLDIIATSVNDELISDSLAVIAALAESSEGSNTILHASALPLLIRTLNSATSSPGKEYCVSALRSLCNHGGEEVVATLAGDRSIAGSLYSIVTEGSAAAGKKARSLLKILHKFRENDVGVNSAVDQERSVRVW
Homology
BLAST of Cp4.1LG20g02300 vs. ExPASy Swiss-Prot
Match: O80742 (U-box domain-containing protein 19 OS=Arabidopsis thaliana OX=3702 GN=PUB19 PE=2 SV=1)

HSP 1 Score: 401.7 bits (1031), Expect = 1.3e-110
Identity = 228/575 (39.65%), Postives = 354/575 (61.57%), Query Frame = 0

Query: 1   MKSQFVATQFWVLIRALATALDVLPLNRIDTSDEVKELVELVVKQARISKFGLDKDDELM 60
           M S  V+  F  L R+++T+LD  P+  +D   EV EL+ LV++Q R S+   D+DD+  
Sbjct: 119 MNSGQVSAHFRDLTRSISTSLDTFPVRSVDLPGEVNELIYLVMRQTRKSEARPDRDDKRA 178

Query: 61  MKRLQSILLQFEKGIEPDLTAIKRVLSYLEIRRWSDCNKEIKFLEEEIDFQYSDLKERDV 120
           +  +      FE  I P+   I RVL ++ +R+W DC KEI FL EEI          ++
Sbjct: 179 IDSVYWFFNLFENRINPNSDEILRVLDHIGVRKWRDCVKEIDFLREEI--SVGKKSNIEI 238

Query: 121 QILSSLVGFMSYSRLTLFEALDFWDKNQTDIKCNPETLSCLNPQDFRCPISLELMIDPVT 180
           ++LS+L+GF+ Y R  +   +D  D+ +   + +   +  LN  D RCPISLE+M DPV 
Sbjct: 239 ELLSNLMGFICYCRCVILRGIDVDDEEKDKEEDDLMMVRSLNVDDLRCPISLEIMSDPVV 298

Query: 181 VSTGQTYDRASIQKWLSAGNLLCPKTGERLTSSELVPNTSLKKLIHQFCVDNGISLAKFN 240
           + +G TYDR+SI KW ++GN+ CPKTG+ L S+ LV N S+K++I  +   NG+ + +  
Sbjct: 299 LESGHTYDRSSITKWFASGNITCPKTGKTLVSTVLVDNFSVKQVIQSYSKQNGVVMGQKG 358

Query: 241 ARSHDITRTIIPGSPAAAEAMKFSSEYLLRRLVFGTDTEKNKAAYEIRLLAKSNIFNRSC 300
            +  D+  ++     AA EA K ++E+L   L+ G + E  KA  EIR+L K++ F RSC
Sbjct: 359 KKKVDVAESL-----AAEEAGKLTAEFLAGELIKGDEEEMVKALVEIRILTKTSTFYRSC 418

Query: 301 LIKVGIIPSLLNLLSSLDKSTQENAIAAVLKLSKHSTGKILVV--ENGGLVPILSVLKSG 360
           L++ G++ SL+ +L S D   QENA+A ++ LSK   GK  +V  + GGL  I+ VL  G
Sbjct: 419 LVEAGVVESLMKILRSDDPRIQENAMAGIMNLSKDIAGKTRIVGEDGGGLRLIVEVLNDG 478

Query: 361 FCLESRQLAAATLFYLSSVKEYRKLIGETPDAITGLIELIK--EGTTCGKKNAVVAIFGL 420
              ESRQ AAA LFYLSS+ +Y +LIGE  DAI GL+ ++K  +     K+NA++AI  L
Sbjct: 479 ARRESRQYAAAALFYLSSLGDYSRLIGEISDAIPGLVRIVKSCDYGDSAKRNALIAIRSL 538

Query: 421 LL-YPKNNKTVLNSGVVPILLDII-ATSVNDELISDSLAVIAALAESSEGSNTILHASAL 480
           L+  P N+  +L +G+VP+LLD++ +  ++D + +DS+A++A +AE  +G  ++L    L
Sbjct: 539 LMNQPDNHWRILAAGIVPVLLDLVKSEEISDGVTADSMAILAKMAEYPDGMISVLRRGGL 598

Query: 481 PLLIRTLNSATSSPG-KEYCVSALRSLCNHGGEEVVATLAGDRSIAGSLYSIVTEGSAAA 540
            L ++ L S+  SP  K++CV+ L +LC++GG +VV +LA + SI GSLY+  + G    
Sbjct: 599 KLAVKILGSSEVSPATKQHCVALLLNLCHNGGSDVVGSLAKNPSIMGSLYTASSNGELGG 658

Query: 541 GKKARSLLKILHKFRENDVGVNSAV-DQERSVRVW 568
           GKKA +L+K++H+F+E   G    V ++ER V  W
Sbjct: 659 GKKASALIKMIHEFQERKTGPGEPVLERERFVHAW 686

BLAST of Cp4.1LG20g02300 vs. ExPASy Swiss-Prot
Match: Q9XIJ5 (U-box domain-containing protein 18 OS=Arabidopsis thaliana OX=3702 GN=PUB18 PE=1 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 6.2e-108
Identity = 234/584 (40.07%), Postives = 358/584 (61.30%), Query Frame = 0

Query: 1   MKSQFVATQFWVLIRALATALDVLPLNRIDTSDEVKELVELVVKQARISKFGL--DKDDE 60
           M S  V+    VL R+++T+L   P+  +D + EV EL++LVV+QAR  K+G+  + +D+
Sbjct: 120 MNSDQVSDHLRVLTRSISTSLSAFPVASVDLTTEVNELIDLVVRQAR--KYGVQPETNDK 179

Query: 61  LMMKRLQSILLQFEKGIEPDLTAIKRVLSYLEIRRWSDCNKEIKFLEEEIDFQYSDLKER 120
             +  +  IL  F   + PD   I R+L ++ IR+W DC KEI FL EEID +  D K++
Sbjct: 180 RAVSSINRILALFVNRVVPDPDEINRILDHVGIRKWGDCVKEINFLGEEIDAERLDEKKK 239

Query: 121 ----DVQILSSLVGFMSYSR---LTLFEALDFWDKNQTDIKCNPETLSCLNPQDFRCPIS 180
                V++LSSL+GF+ Y R   L   E  D  + ++  IK + + +  L  +D  CPIS
Sbjct: 240 KSSDQVELLSSLMGFICYCRCIILGRIERDDHHNHHEDGIKKDHDLIRGLKVEDLLCPIS 299

Query: 181 LELMIDPVTVSTGQTYDRASIQKWLSAGNLLCPKTGERLTSSELVPNTSLKKLIHQFCVD 240
           LE+M DPV + TG TYDR+SI KW  +GN+ CP TG+ LTS+ELV N S++++I + C  
Sbjct: 300 LEIMTDPVVIETGHTYDRSSITKWFGSGNITCPITGKILTSTELVDNVSVRQVIRKHCKT 359

Query: 241 NGISLAKFNAR--SHDITRTIIPGSPAAAEAMKFSSEYLLRRLVFGTDTEKNKAAYEIRL 300
           NGI LA  + R  SHD    ++P S AA  A K  +++L   L+ G +    +A  EIR+
Sbjct: 360 NGIVLAGISRRRKSHD---DVVPESLAAKGAGKLIAKFLTSELINGGEEMIYRAVREIRV 419

Query: 301 LAKSNIFNRSCLIKVGIIPSLLNLLSSLDKSTQENAIAAVLKLSKHSTGKILVVENGGLV 360
             K++ FNRSCL+K G +  LL LLSS+D   QENA+A +L LSKH TGK  +    GL 
Sbjct: 420 QTKTSSFNRSCLVKAGAVTPLLKLLSSVDIRIQENAMAGILNLSKHVTGKSKIA-GEGLK 479

Query: 361 PILSVLKSGFCLESRQLAAATLFYLSSVKEYRKLIGETPDAITGLIELIK--EGTTCGKK 420
            ++ +L  G   E+R  +A+ LFYLSSV++Y +LIGE PDAI GL+ ++K  +     K+
Sbjct: 480 ILVEILNEGAKTETRLYSASALFYLSSVEDYSRLIGENPDAIPGLMNIVKGDDYGDSAKR 539

Query: 421 NAVVAIFGLLLYPKNNKTVLNSGVVPILLDIIAT-SVNDELISDSLAVIAALAESSEGSN 480
           +A++A+ GLL+   N+  VL +G VPILLD++ +  ++  L +D LA +A LAE  +G+ 
Sbjct: 540 SALLAVMGLLMQSDNHWRVLAAGAVPILLDLLRSGEISGGLTADCLATLAKLAEYPDGTI 599

Query: 481 TILHASALPLLIRTLNSATSSP--GKEYCVSALRSLCNHGGEEVVATLAGDRSIAGSLYS 540
            ++    L L ++ L+S+  SP   K++CV  + +LC +GG +VV  L  +  + GSLY+
Sbjct: 600 GVIRRGGLKLAVKILSSSEDSPVAVKQHCVGLILNLCLNGGRDVVGVLVKNSLVMGSLYT 659

Query: 541 IVTEGSAAAGKKARSLLKILHKFRENDVG-VNSAVDQERSVRVW 568
           +++ G     KKA +L++++H+F+E   G V   + + R V  W
Sbjct: 660 VLSNGEYGGSKKASALIRMIHEFQERKTGSVEPNLQRGRFVHAW 697

BLAST of Cp4.1LG20g02300 vs. ExPASy Swiss-Prot
Match: E4NKF8 (U-box domain-containing protein 1 OS=Medicago truncatula OX=3880 GN=PUB1 PE=1 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 3.9e-78
Identity = 193/581 (33.22%), Postives = 323/581 (55.59%), Query Frame = 0

Query: 5   FVATQFWVLIRALATALDVLPLNRIDTSDEVKELVELVVKQARISKFGLDKDDELMMKR- 64
           F++ QF+VL++ +  ALD+LPLN ++ + ++KE V+L+ KQ++  +  L  D   + +R 
Sbjct: 108 FISNQFFVLVKEMGRALDILPLNLLNVAQDIKEQVDLLHKQSKRVELELFIDPREVQRRE 167

Query: 65  ----------LQSILLQFEKGIEPDLTAIKRVLSYLEIRRWSDCNKEIKFLEEEIDFQYS 124
                     LQ+      KG   D   ++ ++  + +R  SD  +EI  LE E   Q  
Sbjct: 168 NLFEVMSKNCLQNKKTNNNKGF-IDFVKVEEIMCSIGLRTLSDYVEEISKLEVEAQNQAG 227

Query: 125 DLKERDVQILSSLVGFMSYSR-----------------LTLFEALDFWDKNQTDIKCNPE 184
                 V  +++L+  +SY++                 + L+      D + +      +
Sbjct: 228 TGGLIVVSNINNLMSLVSYTKSMVFRNDGESEECKPISMFLYNKSKIHDNDSSSSSSFSQ 287

Query: 185 TLSCLN-PQDFRCPISLELMIDPVTVSTGQTYDRASIQKWLSAGNLLCPKTGERLTSSEL 244
           ++  +N P +FRCPISL+LM DPV VS+G TYDR SI +W+++G+  CPK+G+RL  + L
Sbjct: 288 SMMTVNIPDEFRCPISLDLMRDPVIVSSGHTYDRISIAEWINSGHHTCPKSGQRLIHTAL 347

Query: 245 VPNTSLKKLIHQFCVDNGISLAK-------FNARSHDITRTI--IPGSPAAAEAMKFSSE 304
           +PN +LK L+HQ+C +N + + +        +++ H     I  I  + A+ +A+K ++E
Sbjct: 348 IPNYALKSLVHQWCYENNVKMNEAITKNNNSSSKRHKNENAIDHISENKASKDAVKMTAE 407

Query: 305 YLLRRLVFGTDTEKNKAAYEIRLLAKSNIFNRSCLIKVGIIPSLLNLLSSLDKSTQENAI 364
           +L+ +L  G+   + ++AYEIRLLAK+ + NR  + +VG IP L+ LL S D   QE+ +
Sbjct: 408 FLVGKLATGSTDIQRQSAYEIRLLAKTGMDNRRIIAEVGAIPFLVTLLVSKDSRIQEHVV 467

Query: 365 AAVLKLSKHSTGKILVVENGGLVPILSVLKSGFCLESRQLAAATLFYLSSVKEYRKLIGE 424
            A+  LS +   KIL++  G +  I+ VL+ G  +E+R+ AAA ++ LS + + +  IG 
Sbjct: 468 TALFNLSIYDNNKILIMAAGAIDNIVEVLEFGKTMEARENAAAAIYSLSMIDDCKVQIGA 527

Query: 425 TPDAITGLIELIKEGTTCGKKNAVVAIFGLLLYPKNNKTVLNSGVVPILLDIIATSVNDE 484
           +  AI  L+ L+KEGT  GK++A  A+F L +Y  N  +++ SG V +L++++       
Sbjct: 528 SSRAIPALVGLLKEGTIIGKRDAATALFNLAVYNPNKLSIVKSGAVTLLVELLMDD-KAG 587

Query: 485 LISDSLAVIAALAESSEGSNTILHASAL-PLLIRTLNSATSSPGKEYCVSALRSLCNHGG 544
           +  DSLAV+A L   SEG   I ++ +L PLLI  L    S  GKE  ++ L  LC   G
Sbjct: 588 ITDDSLAVLAVLLGCSEGLEEIKNSKSLVPLLIDLLRFG-SVKGKENSITLLLGLCKEEG 647

Query: 545 EEVVATLAGDRSIAGSLYSIVTEGSAAAGKKARSLLKILHK 547
           E V   L  +     SL S+  +GS  A +KA +LL++L++
Sbjct: 648 ELVAMRLLANPRSIPSLQSLAADGSLRARRKADALLRLLNR 685

BLAST of Cp4.1LG20g02300 vs. ExPASy Swiss-Prot
Match: Q9C7R6 (U-box domain-containing protein 17 OS=Arabidopsis thaliana OX=3702 GN=PUB17 PE=2 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 3.3e-77
Identity = 200/564 (35.46%), Postives = 310/564 (54.96%), Query Frame = 0

Query: 1   MKSQFVATQFWVLIRALATALDVLPLNRIDTSDEVKELVELVVKQARISKFGLDKDDELM 60
           +++  ++  F  L + ++T LDVLP+N +  SD+++E +EL+ +Q+R ++  +DK+DE +
Sbjct: 134 LQNPSISGYFHDLNQEISTLLDVLPVNDLGLSDDIREQIELLQRQSRKARLYIDKNDESL 193

Query: 61  MKRLQSILLQFEKGIEPDLTAIKR-VLSYLEIRRWSDCNKEIKFLEEEIDFQYSDLKERD 120
            +   S L  FE G  P    ++   +  L IR    C  EI+FLEE+I     DL E  
Sbjct: 194 RESFYSFLDGFENGKIPSSVDLRMFFVEKLGIRDSKSCRSEIEFLEEQIVNHDGDL-EPT 253

Query: 121 VQILSSLVGFMSYSRLTLF----EALDFWDKNQTD------IKCNPETLSCLNPQDFRCP 180
             +++  V    Y R  LF    + +++W +N         +           P+DF CP
Sbjct: 254 GSVINGFVAITRYCRFLLFGFEEDGMEWWIENNPKKPRKGFVAQEIGDTFITVPKDFVCP 313

Query: 181 ISLELMIDPVTVSTGQTYDRASIQKWLSAGNLLCPKTGERLTSSELVPNTSLKKLIHQFC 240
           ISL+LM DPV +STGQTYDR SI +W+  G+  CPKTG+ L  S +VPN +LK LI Q+C
Sbjct: 314 ISLDLMTDPVIISTGQTYDRNSIARWIEEGHCTCPKTGQMLMDSRIVPNRALKNLIVQWC 373

Query: 241 VDNGISL-AKFNARSHDITRTIIPGSPAAAEAMKFSSEYLLRRLVFGTDTEKNKAAYEIR 300
             +GIS  ++F    ++   + +P + AA EA K +   L++ L  G+   +  AA EIR
Sbjct: 374 TASGISYESEFTDSPNESFASALP-TKAAVEANKATVSILIKYLADGSQAAQTVAAREIR 433

Query: 301 LLAKSNIFNRSCLIKVGIIPSLLNLLSSLDKSTQENAIAAVLKLSKHSTGKILVVENGG- 360
           LLAK+   NR+ + + G IP L  LL+S +   QEN++ A+L LS +   K  ++E G  
Sbjct: 434 LLAKTGKENRAYIAEAGAIPHLCRLLTSENAIAQENSVTAMLNLSIYEKNKSRIMEEGDC 493

Query: 361 LVPILSVLKSGFCLESRQLAAATLFYLSSVKEYRKLIGETPDAITGLIELIKEGTTCGKK 420
           L  I+SVL SG  +E+++ AAATLF LS+V EY+K I      +  L  L++ GT  GKK
Sbjct: 494 LESIVSVLVSGLTVEAQENAAATLFSLSAVHEYKKRIAIVDQCVEALALLLQNGTPRGKK 553

Query: 421 NAVVAIFGLLLYPKNNKTVLNSGVVPILLDIIATSVNDELISDSLAVIAALAESSEGSNT 480
           +AV A++ L  +P N   ++  G V  L+  +    N+ +  ++   +A L   S G+  
Sbjct: 554 DAVTALYNLSTHPDNCSRMIEGGGVSSLVGALK---NEGVAEEAAGALALLVRQSLGAEA 613

Query: 481 I-LHASALPLLIRTLNSATSSPGKEYCVSALRSLCNHGGEEVVATLAGDRSIAGSLYSIV 540
           I    SA+  L+  +   T   GKE  V+AL  LC  GG  V   +    +IAG L +++
Sbjct: 614 IGKEDSAVAGLMGMMRCGTPR-GKENAVAALLELCRSGGAAVAEKVLRAPAIAGLLQTLL 673

Query: 541 TEGSAAAGKKARSLLKILHKFREN 551
             G+  A +KA SL ++  + REN
Sbjct: 674 FTGTKRARRKAASLARVFQR-REN 690

BLAST of Cp4.1LG20g02300 vs. ExPASy Swiss-Prot
Match: Q9LZW3 (U-box domain-containing protein 16 OS=Arabidopsis thaliana OX=3702 GN=PUB16 PE=2 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 4.8e-68
Identity = 179/565 (31.68%), Postives = 290/565 (51.33%), Query Frame = 0

Query: 1   MKSQFVATQFWVLIRALATALDVLPLNRIDTSDEVKELVELVVKQARISKFGLDKDDELM 60
           ++   VA  F  L+  L+T LD+LPL+  D SD+ ++L+ L+ KQ   S   +D  D  +
Sbjct: 120 LQIDIVAFNFHELVTDLSTVLDILPLHDFDLSDDAQDLISLLTKQCSDSVQFVDARDVAL 179

Query: 61  MKRLQSILLQFEKGIEPDLTAIKRVLSYLEIRRWSDCNKEIKFLEEEIDFQYSDLKERDV 120
            +++   +   +  I PD + + ++ + L +   +    EI+ LE+EI  Q  D   R  
Sbjct: 180 RRKVTDTIAGIKHQISPDHSTLIKIFNDLGLSDSASLTDEIQRLEDEIQDQIDD---RSK 239

Query: 121 QILSSLVGFMSYSRLTLFEALDFWDKNQTDIKCNPETLSCLNPQDFRCPISLELMIDPVT 180
              +SL+G + YS+  L+           D + +        P DFRCPI+LELM DPV 
Sbjct: 240 SAAASLIGLVRYSKCVLYGP----STPAPDFRRHQSLSDANIPADFRCPITLELMRDPVV 299

Query: 181 VSTGQTYDRASIQKWLSAGNLLCPKTGERLTSSELVPNTSLKKLIHQFCVDNGISLAKFN 240
           V+TGQTYDR SI  W+ +G+  CPKTG+ L  + LVPN +LK LI  +C D  I    + 
Sbjct: 300 VATGQTYDRESIDLWIQSGHNTCPKTGQVLKHTSLVPNRALKNLIVLWCRDQKIPFELYG 359

Query: 241 ARSHDITRTIIPGSPA----AAEAMKFSSEYLLRRLVFGTDTEKNKAAYEIRLLAKSNIF 300
                       G PA    A E  K    +L+ +L   +  + N   +E+R LAKS+  
Sbjct: 360 DGG---------GEPAPCKEAVEFTKMMVSFLIEKL---SVADSNGVVFELRALAKSDTV 419

Query: 301 NRSCLIKVGIIPSLLNLLSSLDKSTQENAIAAVLKLSKHSTGKILVVE-NGGLVPILSVL 360
            R+C+ + G IP L+  L++   S Q NA+  +L LS     K  ++E +G L  ++ VL
Sbjct: 420 ARACIAEAGAIPKLVRYLATECPSLQINAVTTILNLSILEQNKTRIMETDGALNGVIEVL 479

Query: 361 KSGFCLESRQLAAATLFYLSSVKEYRKLIGETPDAITGLIELIKEGTTCGKKNAVVAIFG 420
           +SG   E++  AAATLF L+ V  YR+ +G     ++GL++L K+G T  K++A+VAI  
Sbjct: 480 RSGATWEAKANAAATLFSLAGVSAYRRRLGRKARVVSGLVDLAKQGPTSSKRDALVAILN 539

Query: 421 LLLYPKNNKTVLNSGVVPILLDIIATSVNDELISDSLAVIAALAESSEGSNTILHASALP 480
           L+   +N    + +GV+    D        EL  +++AV+ A+     G   +  A +L 
Sbjct: 540 LVAERENVGRFVEAGVMGAAGDAF-----QELPEEAVAVVEAVVRRG-GLMAVSAAFSLI 599

Query: 481 LLIRTLNSATSSPGKEYCVSALRSLCNHGGEEVVATLAGDRSIAGSLYSIVTEGSAAAGK 540
            L+  +    +   +E   + L ++C  GG E+VA +A    I   ++ ++  G+A  G+
Sbjct: 600 RLLGEVMREGADTTRESAAATLVTMCRKGGSELVAEMAAIPGIERVIWEMIGAGTARGGR 659

Query: 541 KARSLLKILHKFRENDVGVNSAVDQ 561
           KA SL++ L ++   D    +A  Q
Sbjct: 660 KAASLMRYLRRWAAGDTHNTAAETQ 659

BLAST of Cp4.1LG20g02300 vs. NCBI nr
Match: XP_023519714.1 (U-box domain-containing protein 19-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1080 bits (2792), Expect = 0.0
Identity = 567/567 (100.00%), Postives = 567/567 (100.00%), Query Frame = 0

Query: 1   MKSQFVATQFWVLIRALATALDVLPLNRIDTSDEVKELVELVVKQARISKFGLDKDDELM 60
           MKSQFVATQFWVLIRALATALDVLPLNRIDTSDEVKELVELVVKQARISKFGLDKDDELM
Sbjct: 116 MKSQFVATQFWVLIRALATALDVLPLNRIDTSDEVKELVELVVKQARISKFGLDKDDELM 175

Query: 61  MKRLQSILLQFEKGIEPDLTAIKRVLSYLEIRRWSDCNKEIKFLEEEIDFQYSDLKERDV 120
           MKRLQSILLQFEKGIEPDLTAIKRVLSYLEIRRWSDCNKEIKFLEEEIDFQYSDLKERDV
Sbjct: 176 MKRLQSILLQFEKGIEPDLTAIKRVLSYLEIRRWSDCNKEIKFLEEEIDFQYSDLKERDV 235

Query: 121 QILSSLVGFMSYSRLTLFEALDFWDKNQTDIKCNPETLSCLNPQDFRCPISLELMIDPVT 180
           QILSSLVGFMSYSRLTLFEALDFWDKNQTDIKCNPETLSCLNPQDFRCPISLELMIDPVT
Sbjct: 236 QILSSLVGFMSYSRLTLFEALDFWDKNQTDIKCNPETLSCLNPQDFRCPISLELMIDPVT 295

Query: 181 VSTGQTYDRASIQKWLSAGNLLCPKTGERLTSSELVPNTSLKKLIHQFCVDNGISLAKFN 240
           VSTGQTYDRASIQKWLSAGNLLCPKTGERLTSSELVPNTSLKKLIHQFCVDNGISLAKFN
Sbjct: 296 VSTGQTYDRASIQKWLSAGNLLCPKTGERLTSSELVPNTSLKKLIHQFCVDNGISLAKFN 355

Query: 241 ARSHDITRTIIPGSPAAAEAMKFSSEYLLRRLVFGTDTEKNKAAYEIRLLAKSNIFNRSC 300
           ARSHDITRTIIPGSPAAAEAMKFSSEYLLRRLVFGTDTEKNKAAYEIRLLAKSNIFNRSC
Sbjct: 356 ARSHDITRTIIPGSPAAAEAMKFSSEYLLRRLVFGTDTEKNKAAYEIRLLAKSNIFNRSC 415

Query: 301 LIKVGIIPSLLNLLSSLDKSTQENAIAAVLKLSKHSTGKILVVENGGLVPILSVLKSGFC 360
           LIKVGIIPSLLNLLSSLDKSTQENAIAAVLKLSKHSTGKILVVENGGLVPILSVLKSGFC
Sbjct: 416 LIKVGIIPSLLNLLSSLDKSTQENAIAAVLKLSKHSTGKILVVENGGLVPILSVLKSGFC 475

Query: 361 LESRQLAAATLFYLSSVKEYRKLIGETPDAITGLIELIKEGTTCGKKNAVVAIFGLLLYP 420
           LESRQLAAATLFYLSSVKEYRKLIGETPDAITGLIELIKEGTTCGKKNAVVAIFGLLLYP
Sbjct: 476 LESRQLAAATLFYLSSVKEYRKLIGETPDAITGLIELIKEGTTCGKKNAVVAIFGLLLYP 535

Query: 421 KNNKTVLNSGVVPILLDIIATSVNDELISDSLAVIAALAESSEGSNTILHASALPLLIRT 480
           KNNKTVLNSGVVPILLDIIATSVNDELISDSLAVIAALAESSEGSNTILHASALPLLIRT
Sbjct: 536 KNNKTVLNSGVVPILLDIIATSVNDELISDSLAVIAALAESSEGSNTILHASALPLLIRT 595

Query: 481 LNSATSSPGKEYCVSALRSLCNHGGEEVVATLAGDRSIAGSLYSIVTEGSAAAGKKARSL 540
           LNSATSSPGKEYCVSALRSLCNHGGEEVVATLAGDRSIAGSLYSIVTEGSAAAGKKARSL
Sbjct: 596 LNSATSSPGKEYCVSALRSLCNHGGEEVVATLAGDRSIAGSLYSIVTEGSAAAGKKARSL 655

Query: 541 LKILHKFRENDVGVNSAVDQERSVRVW 567
           LKILHKFRENDVGVNSAVDQERSVRVW
Sbjct: 656 LKILHKFRENDVGVNSAVDQERSVRVW 682

BLAST of Cp4.1LG20g02300 vs. NCBI nr
Match: KAG7019826.1 (U-box domain-containing protein 19, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1065 bits (2755), Expect = 0.0
Identity = 560/567 (98.77%), Postives = 561/567 (98.94%), Query Frame = 0

Query: 1   MKSQFVATQFWVLIRALATALDVLPLNRIDTSDEVKELVELVVKQARISKFGLDKDDELM 60
           MKSQFVATQFWVLIRALATALDVLPLNRIDTSDEVKELVELVVKQARISKFGLDKDDEL 
Sbjct: 115 MKSQFVATQFWVLIRALATALDVLPLNRIDTSDEVKELVELVVKQARISKFGLDKDDELT 174

Query: 61  MKRLQSILLQFEKGIEPDLTAIKRVLSYLEIRRWSDCNKEIKFLEEEIDFQYSDLKERDV 120
           MKRLQSILLQFEKGIEPDLTAIKRVLSYLEIRRWSDCNKEIKFLEEEIDFQYSDLKERDV
Sbjct: 175 MKRLQSILLQFEKGIEPDLTAIKRVLSYLEIRRWSDCNKEIKFLEEEIDFQYSDLKERDV 234

Query: 121 QILSSLVGFMSYSRLTLFEALDFWDKNQTDIKCNPETLSCLNPQDFRCPISLELMIDPVT 180
           QILSSLVGFMSYSRLTLFEALDFWDKNQTDIKCNPETLSCLNPQDFRCPISLELMIDPVT
Sbjct: 235 QILSSLVGFMSYSRLTLFEALDFWDKNQTDIKCNPETLSCLNPQDFRCPISLELMIDPVT 294

Query: 181 VSTGQTYDRASIQKWLSAGNLLCPKTGERLTSSELVPNTSLKKLIHQFCVDNGISLAKFN 240
           VSTGQTYDRASIQKWLSAGNLLCPKTGERLTSSELVPNTSLKKLIHQFCVDNGISLAKFN
Sbjct: 295 VSTGQTYDRASIQKWLSAGNLLCPKTGERLTSSELVPNTSLKKLIHQFCVDNGISLAKFN 354

Query: 241 ARSHDITRTIIPGSPAAAEAMKFSSEYLLRRLVFGTDTEKNKAAYEIRLLAKSNIFNRSC 300
           ARSHDITRTIIPGSPAAAEAMKFSSEYLLR+LVFGT TEKNKAAYEIRLLAKSNIFNRSC
Sbjct: 355 ARSHDITRTIIPGSPAAAEAMKFSSEYLLRQLVFGTSTEKNKAAYEIRLLAKSNIFNRSC 414

Query: 301 LIKVGIIPSLLNLLSSLDKSTQENAIAAVLKLSKHSTGKILVVENGGLVPILSVLKSGFC 360
           LIKVGIIP LLNLLSSLDKSTQENAIAAVLKLSKHSTGKILVVENGGL PILSVLKSGFC
Sbjct: 415 LIKVGIIPPLLNLLSSLDKSTQENAIAAVLKLSKHSTGKILVVENGGLAPILSVLKSGFC 474

Query: 361 LESRQLAAATLFYLSSVKEYRKLIGETPDAITGLIELIKEGTTCGKKNAVVAIFGLLLYP 420
           LESRQLAAATLFYLSSVKEYRKLIGE PDAITGLIELIKEGTTCGKKNAVVAIFGLLLYP
Sbjct: 475 LESRQLAAATLFYLSSVKEYRKLIGEMPDAITGLIELIKEGTTCGKKNAVVAIFGLLLYP 534

Query: 421 KNNKTVLNSGVVPILLDIIATSVNDELISDSLAVIAALAESSEGSNTILHASALPLLIRT 480
           KNNKTVLNSGVVPILLDIIATSVNDELISDSLAVIAALAESSEGS TILHASALPLLIRT
Sbjct: 535 KNNKTVLNSGVVPILLDIIATSVNDELISDSLAVIAALAESSEGSTTILHASALPLLIRT 594

Query: 481 LNSATSSPGKEYCVSALRSLCNHGGEEVVATLAGDRSIAGSLYSIVTEGSAAAGKKARSL 540
           LNSATSSPGKEYCVSALRSLCNHGGEEVVATLAGDRSIAGSLYSIVTEGSAAAGKKARSL
Sbjct: 595 LNSATSSPGKEYCVSALRSLCNHGGEEVVATLAGDRSIAGSLYSIVTEGSAAAGKKARSL 654

Query: 541 LKILHKFRENDVGVNSAVDQERSVRVW 567
           LKILHKFRENDVGVNSAVDQERSVRVW
Sbjct: 655 LKILHKFRENDVGVNSAVDQERSVRVW 681

BLAST of Cp4.1LG20g02300 vs. NCBI nr
Match: XP_022923709.1 (LOW QUALITY PROTEIN: U-box domain-containing protein 19-like [Cucurbita moschata])

HSP 1 Score: 1057 bits (2733), Expect = 0.0
Identity = 554/567 (97.71%), Postives = 559/567 (98.59%), Query Frame = 0

Query: 1   MKSQFVATQFWVLIRALATALDVLPLNRIDTSDEVKELVELVVKQARISKFGLDKDDELM 60
           MKSQFVATQFWVLIRALATALDVLPLNRIDT D+VKELVELVVKQARISKFGLDKDDEL 
Sbjct: 115 MKSQFVATQFWVLIRALATALDVLPLNRIDTGDDVKELVELVVKQARISKFGLDKDDELT 174

Query: 61  MKRLQSILLQFEKGIEPDLTAIKRVLSYLEIRRWSDCNKEIKFLEEEIDFQYSDLKERDV 120
           MKRLQSILLQFEKGIEPDLTAIKRVLSYLEIRRWSDCNKEIKFLEEEIDFQYSDLKERDV
Sbjct: 175 MKRLQSILLQFEKGIEPDLTAIKRVLSYLEIRRWSDCNKEIKFLEEEIDFQYSDLKERDV 234

Query: 121 QILSSLVGFMSYSRLTLFEALDFWDKNQTDIKCNPETLSCLNPQDFRCPISLELMIDPVT 180
           QILSSLVGF+SYSRLTLFEALDFWDKNQTDIKCNPETLSCLNPQDFRCPISLELMIDPVT
Sbjct: 235 QILSSLVGFLSYSRLTLFEALDFWDKNQTDIKCNPETLSCLNPQDFRCPISLELMIDPVT 294

Query: 181 VSTGQTYDRASIQKWLSAGNLLCPKTGERLTSSELVPNTSLKKLIHQFCVDNGISLAKFN 240
           VSTGQTYDRASIQKWLSAGNLLCPKTG+RLTSSELVPNTSLKKLIHQFCVDNGISLAKFN
Sbjct: 295 VSTGQTYDRASIQKWLSAGNLLCPKTGKRLTSSELVPNTSLKKLIHQFCVDNGISLAKFN 354

Query: 241 ARSHDITRTIIPGSPAAAEAMKFSSEYLLRRLVFGTDTEKNKAAYEIRLLAKSNIFNRSC 300
           ARSHDITRTIIPGSPAAAEAMKFSSEYLLR+LVFGT TEKNKAAYEIRLLAKSNIFNRSC
Sbjct: 355 ARSHDITRTIIPGSPAAAEAMKFSSEYLLRQLVFGTSTEKNKAAYEIRLLAKSNIFNRSC 414

Query: 301 LIKVGIIPSLLNLLSSLDKSTQENAIAAVLKLSKHSTGKILVVENGGLVPILSVLKSGFC 360
           LIKVGIIP LLNLLSSLDKSTQENAIAAVLKLSKHSTGKILVVENGGL PILSVLKSGFC
Sbjct: 415 LIKVGIIPPLLNLLSSLDKSTQENAIAAVLKLSKHSTGKILVVENGGLAPILSVLKSGFC 474

Query: 361 LESRQLAAATLFYLSSVKEYRKLIGETPDAITGLIELIKEGTTCGKKNAVVAIFGLLLYP 420
           LESRQLAAATLFYLSSVKEYRKLIGE PDAITGLIELIKEGTTCGKKNAVVAIFGLLLYP
Sbjct: 475 LESRQLAAATLFYLSSVKEYRKLIGEMPDAITGLIELIKEGTTCGKKNAVVAIFGLLLYP 534

Query: 421 KNNKTVLNSGVVPILLDIIATSVNDELISDSLAVIAALAESSEGSNTILHASALPLLIRT 480
           KNNKTVLNSGVVPILLDIIATSVNDELISDSLAVIAALAESSEGS TILHASALPLLIRT
Sbjct: 535 KNNKTVLNSGVVPILLDIIATSVNDELISDSLAVIAALAESSEGSTTILHASALPLLIRT 594

Query: 481 LNSATSSPGKEYCVSALRSLCNHGGEEVVATLAGDRSIAGSLYSIVTEGSAAAGKKARSL 540
           LNSATSSPGKEYCVSALRSLCNHGGEEVVATLAGDRSIAGSLYSIVTEG A+AGKKARSL
Sbjct: 595 LNSATSSPGKEYCVSALRSLCNHGGEEVVATLAGDRSIAGSLYSIVTEGCASAGKKARSL 654

Query: 541 LKILHKFRENDVGVNSAVDQERSVRVW 567
           LKILHKFRENDVGVNSAVDQERSVRVW
Sbjct: 655 LKILHKFRENDVGVNSAVDQERSVRVW 681

BLAST of Cp4.1LG20g02300 vs. NCBI nr
Match: XP_023000658.1 (U-box domain-containing protein 19-like [Cucurbita maxima])

HSP 1 Score: 1056 bits (2730), Expect = 0.0
Identity = 555/567 (97.88%), Postives = 557/567 (98.24%), Query Frame = 0

Query: 1   MKSQFVATQFWVLIRALATALDVLPLNRIDTSDEVKELVELVVKQARISKFGLDKDDELM 60
           MKSQFVATQFWVLIRALATALDVLPLNRIDTSDEVKELVELVVKQARISKFGLDKDDEL 
Sbjct: 115 MKSQFVATQFWVLIRALATALDVLPLNRIDTSDEVKELVELVVKQARISKFGLDKDDELT 174

Query: 61  MKRLQSILLQFEKGIEPDLTAIKRVLSYLEIRRWSDCNKEIKFLEEEIDFQYSDLKERDV 120
           MKRLQSILLQFEKGIEPDLTAIKRVLSYLEIRRWSDCNKEIKFLEEEIDFQYSDLKERDV
Sbjct: 175 MKRLQSILLQFEKGIEPDLTAIKRVLSYLEIRRWSDCNKEIKFLEEEIDFQYSDLKERDV 234

Query: 121 QILSSLVGFMSYSRLTLFEALDFWDKNQTDIKCNPETLSCLNPQDFRCPISLELMIDPVT 180
            ILSSL+GFMSYSRLTLFEALDFWDKNQTDIKCNPETLSCLNPQDFRCPISLELMIDPVT
Sbjct: 235 HILSSLMGFMSYSRLTLFEALDFWDKNQTDIKCNPETLSCLNPQDFRCPISLELMIDPVT 294

Query: 181 VSTGQTYDRASIQKWLSAGNLLCPKTGERLTSSELVPNTSLKKLIHQFCVDNGISLAKFN 240
           VSTGQTYDRASIQKWLSAGNLLCPKTGERLTSSELVPNTSLKKLIHQFCVDNGISLAKFN
Sbjct: 295 VSTGQTYDRASIQKWLSAGNLLCPKTGERLTSSELVPNTSLKKLIHQFCVDNGISLAKFN 354

Query: 241 ARSHDITRTIIPGSPAAAEAMKFSSEYLLRRLVFGTDTEKNKAAYEIRLLAKSNIFNRSC 300
           ARSHDITRTIIPGSPAAAEAMKFSSEYLLRRLVFGT  EKNKAAYEIRLLAKSNIFNRSC
Sbjct: 355 ARSHDITRTIIPGSPAAAEAMKFSSEYLLRRLVFGTSKEKNKAAYEIRLLAKSNIFNRSC 414

Query: 301 LIKVGIIPSLLNLLSSLDKSTQENAIAAVLKLSKHSTGKILVVENGGLVPILSVLKSGFC 360
           LIK GIIP L+NLLSSLDKSTQENAIAAVLKLSKHSTGKILVVENGGL PILSVLKSGFC
Sbjct: 415 LIKAGIIPPLVNLLSSLDKSTQENAIAAVLKLSKHSTGKILVVENGGLAPILSVLKSGFC 474

Query: 361 LESRQLAAATLFYLSSVKEYRKLIGETPDAITGLIELIKEGTTCGKKNAVVAIFGLLLYP 420
           LESRQLAAATLFYLSSVKEYRKLIGE PDAITGLIELIKEGTTCGKKNAVVAIFGLLLYP
Sbjct: 475 LESRQLAAATLFYLSSVKEYRKLIGEMPDAITGLIELIKEGTTCGKKNAVVAIFGLLLYP 534

Query: 421 KNNKTVLNSGVVPILLDIIATSVNDELISDSLAVIAALAESSEGSNTILHASALPLLIRT 480
           KNNKTVLNSGVVPILLDIIATSVNDELISDSLAVIAALAESSEGS TILHASAL LLIRT
Sbjct: 535 KNNKTVLNSGVVPILLDIIATSVNDELISDSLAVIAALAESSEGSTTILHASALTLLIRT 594

Query: 481 LNSATSSPGKEYCVSALRSLCNHGGEEVVATLAGDRSIAGSLYSIVTEGSAAAGKKARSL 540
           LNSATSSPGKEYCVSALRSLCNHGGEEVVATLAGDRSIAGSLYSIVTEGSAAAGKKARSL
Sbjct: 595 LNSATSSPGKEYCVSALRSLCNHGGEEVVATLAGDRSIAGSLYSIVTEGSAAAGKKARSL 654

Query: 541 LKILHKFRENDVGVNSAVDQERSVRVW 567
           LKILHKFRENDVGVNSAVDQERSVRVW
Sbjct: 655 LKILHKFRENDVGVNSAVDQERSVRVW 681

BLAST of Cp4.1LG20g02300 vs. NCBI nr
Match: KAG6584229.1 (U-box domain-containing protein 19, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1053 bits (2723), Expect = 0.0
Identity = 555/564 (98.40%), Postives = 557/564 (98.76%), Query Frame = 0

Query: 1   MKSQFVATQFWVLIRALATALDVLPLNRIDTSDEVKELVELVVKQARISKFGLDKDDELM 60
           MKSQFVATQFWVLIRALATALDVLPLNRIDTSDEVKELVELVVKQARISKFGLDKDDEL 
Sbjct: 115 MKSQFVATQFWVLIRALATALDVLPLNRIDTSDEVKELVELVVKQARISKFGLDKDDELT 174

Query: 61  MKRLQSILLQFEKGIEPDLTAIKRVLSYLEIRRWSDCNKEIKFLEEEIDFQYSDLKERDV 120
           MKRLQSILLQFEK IEPDLTAIKRVLSYLEIRRWSDCNKEIKFLEEEIDFQYSDLKERDV
Sbjct: 175 MKRLQSILLQFEKRIEPDLTAIKRVLSYLEIRRWSDCNKEIKFLEEEIDFQYSDLKERDV 234

Query: 121 QILSSLVGFMSYSRLTLFEALDFWDKNQTDIKCNPETLSCLNPQDFRCPISLELMIDPVT 180
           QILSSLVGFMSYSRLTLFEALDFWDKNQTDIKCNPETLSCLNPQDFRCPISLELMIDPVT
Sbjct: 235 QILSSLVGFMSYSRLTLFEALDFWDKNQTDIKCNPETLSCLNPQDFRCPISLELMIDPVT 294

Query: 181 VSTGQTYDRASIQKWLSAGNLLCPKTGERLTSSELVPNTSLKKLIHQFCVDNGISLAKFN 240
           VSTGQTYDRASIQKWLSAGNLLCPKTGERLTSSELVPNTSLKKLIHQFCVDNGISLAKFN
Sbjct: 295 VSTGQTYDRASIQKWLSAGNLLCPKTGERLTSSELVPNTSLKKLIHQFCVDNGISLAKFN 354

Query: 241 ARSHDITRTIIPGSPAAAEAMKFSSEYLLRRLVFGTDTEKNKAAYEIRLLAKSNIFNRSC 300
           ARSHDITRTIIPGSPAAAEAMKFSSEYLLR+LVFGT TEKNKAAYEIRLLAKSNIFNRSC
Sbjct: 355 ARSHDITRTIIPGSPAAAEAMKFSSEYLLRQLVFGTSTEKNKAAYEIRLLAKSNIFNRSC 414

Query: 301 LIKVGIIPSLLNLLSSLDKSTQENAIAAVLKLSKHSTGKILVVENGGLVPILSVLKSGFC 360
           LIKVGIIP LLNLLSSLDKSTQENAIAAVLKLSKHSTGKILVVENGGL PILSVLKSGFC
Sbjct: 415 LIKVGIIPPLLNLLSSLDKSTQENAIAAVLKLSKHSTGKILVVENGGLAPILSVLKSGFC 474

Query: 361 LESRQLAAATLFYLSSVKEYRKLIGETPDAITGLIELIKEGTTCGKKNAVVAIFGLLLYP 420
           LESRQLAAATLFYLSSVKEYRKLIGE PDAITGLIELIKEGTTCGKKNAVVAIFGLLLYP
Sbjct: 475 LESRQLAAATLFYLSSVKEYRKLIGEMPDAITGLIELIKEGTTCGKKNAVVAIFGLLLYP 534

Query: 421 KNNKTVLNSGVVPILLDIIATSVNDELISDSLAVIAALAESSEGSNTILHASALPLLIRT 480
           KNNKTVLNSGVVPILLDIIATSVNDELISDSLAVIAALAESSEGS TILHASALPLLIRT
Sbjct: 535 KNNKTVLNSGVVPILLDIIATSVNDELISDSLAVIAALAESSEGSTTILHASALPLLIRT 594

Query: 481 LNSATSSPGKEYCVSALRSLCNHGGEEVVATLAGDRSIAGSLYSIVTEGSAAAGKKARSL 540
           LNSATSSPGKEYCVSALRSLCNHGGEEVVATLAGDRSIAGSLYSIVTEGSAAAGKKARSL
Sbjct: 595 LNSATSSPGKEYCVSALRSLCNHGGEEVVATLAGDRSIAGSLYSIVTEGSAAAGKKARSL 654

Query: 541 LKILHKFRENDVGVNSAVDQERSV 564
           LKILHKFRENDVGVNSAVDQERS+
Sbjct: 655 LKILHKFRENDVGVNSAVDQERSL 678

BLAST of Cp4.1LG20g02300 vs. ExPASy TrEMBL
Match: A0A6J1E762 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111431336 PE=4 SV=1)

HSP 1 Score: 1057 bits (2733), Expect = 0.0
Identity = 554/567 (97.71%), Postives = 559/567 (98.59%), Query Frame = 0

Query: 1   MKSQFVATQFWVLIRALATALDVLPLNRIDTSDEVKELVELVVKQARISKFGLDKDDELM 60
           MKSQFVATQFWVLIRALATALDVLPLNRIDT D+VKELVELVVKQARISKFGLDKDDEL 
Sbjct: 115 MKSQFVATQFWVLIRALATALDVLPLNRIDTGDDVKELVELVVKQARISKFGLDKDDELT 174

Query: 61  MKRLQSILLQFEKGIEPDLTAIKRVLSYLEIRRWSDCNKEIKFLEEEIDFQYSDLKERDV 120
           MKRLQSILLQFEKGIEPDLTAIKRVLSYLEIRRWSDCNKEIKFLEEEIDFQYSDLKERDV
Sbjct: 175 MKRLQSILLQFEKGIEPDLTAIKRVLSYLEIRRWSDCNKEIKFLEEEIDFQYSDLKERDV 234

Query: 121 QILSSLVGFMSYSRLTLFEALDFWDKNQTDIKCNPETLSCLNPQDFRCPISLELMIDPVT 180
           QILSSLVGF+SYSRLTLFEALDFWDKNQTDIKCNPETLSCLNPQDFRCPISLELMIDPVT
Sbjct: 235 QILSSLVGFLSYSRLTLFEALDFWDKNQTDIKCNPETLSCLNPQDFRCPISLELMIDPVT 294

Query: 181 VSTGQTYDRASIQKWLSAGNLLCPKTGERLTSSELVPNTSLKKLIHQFCVDNGISLAKFN 240
           VSTGQTYDRASIQKWLSAGNLLCPKTG+RLTSSELVPNTSLKKLIHQFCVDNGISLAKFN
Sbjct: 295 VSTGQTYDRASIQKWLSAGNLLCPKTGKRLTSSELVPNTSLKKLIHQFCVDNGISLAKFN 354

Query: 241 ARSHDITRTIIPGSPAAAEAMKFSSEYLLRRLVFGTDTEKNKAAYEIRLLAKSNIFNRSC 300
           ARSHDITRTIIPGSPAAAEAMKFSSEYLLR+LVFGT TEKNKAAYEIRLLAKSNIFNRSC
Sbjct: 355 ARSHDITRTIIPGSPAAAEAMKFSSEYLLRQLVFGTSTEKNKAAYEIRLLAKSNIFNRSC 414

Query: 301 LIKVGIIPSLLNLLSSLDKSTQENAIAAVLKLSKHSTGKILVVENGGLVPILSVLKSGFC 360
           LIKVGIIP LLNLLSSLDKSTQENAIAAVLKLSKHSTGKILVVENGGL PILSVLKSGFC
Sbjct: 415 LIKVGIIPPLLNLLSSLDKSTQENAIAAVLKLSKHSTGKILVVENGGLAPILSVLKSGFC 474

Query: 361 LESRQLAAATLFYLSSVKEYRKLIGETPDAITGLIELIKEGTTCGKKNAVVAIFGLLLYP 420
           LESRQLAAATLFYLSSVKEYRKLIGE PDAITGLIELIKEGTTCGKKNAVVAIFGLLLYP
Sbjct: 475 LESRQLAAATLFYLSSVKEYRKLIGEMPDAITGLIELIKEGTTCGKKNAVVAIFGLLLYP 534

Query: 421 KNNKTVLNSGVVPILLDIIATSVNDELISDSLAVIAALAESSEGSNTILHASALPLLIRT 480
           KNNKTVLNSGVVPILLDIIATSVNDELISDSLAVIAALAESSEGS TILHASALPLLIRT
Sbjct: 535 KNNKTVLNSGVVPILLDIIATSVNDELISDSLAVIAALAESSEGSTTILHASALPLLIRT 594

Query: 481 LNSATSSPGKEYCVSALRSLCNHGGEEVVATLAGDRSIAGSLYSIVTEGSAAAGKKARSL 540
           LNSATSSPGKEYCVSALRSLCNHGGEEVVATLAGDRSIAGSLYSIVTEG A+AGKKARSL
Sbjct: 595 LNSATSSPGKEYCVSALRSLCNHGGEEVVATLAGDRSIAGSLYSIVTEGCASAGKKARSL 654

Query: 541 LKILHKFRENDVGVNSAVDQERSVRVW 567
           LKILHKFRENDVGVNSAVDQERSVRVW
Sbjct: 655 LKILHKFRENDVGVNSAVDQERSVRVW 681

BLAST of Cp4.1LG20g02300 vs. ExPASy TrEMBL
Match: A0A6J1KN84 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111495027 PE=4 SV=1)

HSP 1 Score: 1056 bits (2730), Expect = 0.0
Identity = 555/567 (97.88%), Postives = 557/567 (98.24%), Query Frame = 0

Query: 1   MKSQFVATQFWVLIRALATALDVLPLNRIDTSDEVKELVELVVKQARISKFGLDKDDELM 60
           MKSQFVATQFWVLIRALATALDVLPLNRIDTSDEVKELVELVVKQARISKFGLDKDDEL 
Sbjct: 115 MKSQFVATQFWVLIRALATALDVLPLNRIDTSDEVKELVELVVKQARISKFGLDKDDELT 174

Query: 61  MKRLQSILLQFEKGIEPDLTAIKRVLSYLEIRRWSDCNKEIKFLEEEIDFQYSDLKERDV 120
           MKRLQSILLQFEKGIEPDLTAIKRVLSYLEIRRWSDCNKEIKFLEEEIDFQYSDLKERDV
Sbjct: 175 MKRLQSILLQFEKGIEPDLTAIKRVLSYLEIRRWSDCNKEIKFLEEEIDFQYSDLKERDV 234

Query: 121 QILSSLVGFMSYSRLTLFEALDFWDKNQTDIKCNPETLSCLNPQDFRCPISLELMIDPVT 180
            ILSSL+GFMSYSRLTLFEALDFWDKNQTDIKCNPETLSCLNPQDFRCPISLELMIDPVT
Sbjct: 235 HILSSLMGFMSYSRLTLFEALDFWDKNQTDIKCNPETLSCLNPQDFRCPISLELMIDPVT 294

Query: 181 VSTGQTYDRASIQKWLSAGNLLCPKTGERLTSSELVPNTSLKKLIHQFCVDNGISLAKFN 240
           VSTGQTYDRASIQKWLSAGNLLCPKTGERLTSSELVPNTSLKKLIHQFCVDNGISLAKFN
Sbjct: 295 VSTGQTYDRASIQKWLSAGNLLCPKTGERLTSSELVPNTSLKKLIHQFCVDNGISLAKFN 354

Query: 241 ARSHDITRTIIPGSPAAAEAMKFSSEYLLRRLVFGTDTEKNKAAYEIRLLAKSNIFNRSC 300
           ARSHDITRTIIPGSPAAAEAMKFSSEYLLRRLVFGT  EKNKAAYEIRLLAKSNIFNRSC
Sbjct: 355 ARSHDITRTIIPGSPAAAEAMKFSSEYLLRRLVFGTSKEKNKAAYEIRLLAKSNIFNRSC 414

Query: 301 LIKVGIIPSLLNLLSSLDKSTQENAIAAVLKLSKHSTGKILVVENGGLVPILSVLKSGFC 360
           LIK GIIP L+NLLSSLDKSTQENAIAAVLKLSKHSTGKILVVENGGL PILSVLKSGFC
Sbjct: 415 LIKAGIIPPLVNLLSSLDKSTQENAIAAVLKLSKHSTGKILVVENGGLAPILSVLKSGFC 474

Query: 361 LESRQLAAATLFYLSSVKEYRKLIGETPDAITGLIELIKEGTTCGKKNAVVAIFGLLLYP 420
           LESRQLAAATLFYLSSVKEYRKLIGE PDAITGLIELIKEGTTCGKKNAVVAIFGLLLYP
Sbjct: 475 LESRQLAAATLFYLSSVKEYRKLIGEMPDAITGLIELIKEGTTCGKKNAVVAIFGLLLYP 534

Query: 421 KNNKTVLNSGVVPILLDIIATSVNDELISDSLAVIAALAESSEGSNTILHASALPLLIRT 480
           KNNKTVLNSGVVPILLDIIATSVNDELISDSLAVIAALAESSEGS TILHASAL LLIRT
Sbjct: 535 KNNKTVLNSGVVPILLDIIATSVNDELISDSLAVIAALAESSEGSTTILHASALTLLIRT 594

Query: 481 LNSATSSPGKEYCVSALRSLCNHGGEEVVATLAGDRSIAGSLYSIVTEGSAAAGKKARSL 540
           LNSATSSPGKEYCVSALRSLCNHGGEEVVATLAGDRSIAGSLYSIVTEGSAAAGKKARSL
Sbjct: 595 LNSATSSPGKEYCVSALRSLCNHGGEEVVATLAGDRSIAGSLYSIVTEGSAAAGKKARSL 654

Query: 541 LKILHKFRENDVGVNSAVDQERSVRVW 567
           LKILHKFRENDVGVNSAVDQERSVRVW
Sbjct: 655 LKILHKFRENDVGVNSAVDQERSVRVW 681

BLAST of Cp4.1LG20g02300 vs. ExPASy TrEMBL
Match: A0A5A7UP25 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G00600 PE=4 SV=1)

HSP 1 Score: 968 bits (2503), Expect = 0.0
Identity = 508/567 (89.59%), Postives = 533/567 (94.00%), Query Frame = 0

Query: 1   MKSQFVATQFWVLIRALATALDVLPLNRIDTSDEVKELVELVVKQARISKFGLDKDDELM 60
           MKSQFVATQFWVLIRALATALDVLPLNRIDTSDEVKELVELV KQARI+KFGLDKDDEL 
Sbjct: 115 MKSQFVATQFWVLIRALATALDVLPLNRIDTSDEVKELVELVAKQARIAKFGLDKDDELT 174

Query: 61  MKRLQSILLQFEKGIEPDLTAIKRVLSYLEIRRWSDCNKEIKFLEEEIDFQYSDLKERDV 120
           MKRLQSILLQFEKGIEPDLTAIKRVL+YLEIRRWSDCNKEIKFLEEEIDFQY DLKERDV
Sbjct: 175 MKRLQSILLQFEKGIEPDLTAIKRVLNYLEIRRWSDCNKEIKFLEEEIDFQYLDLKERDV 234

Query: 121 QILSSLVGFMSYSRLTLFEALDFWDKNQTDIKCNPETLSCLNPQDFRCPISLELMIDPVT 180
           QILSSLVGF+ YSR+TLFEALDF DKNQ + KCNPE LSCLNP+DFRCPISLELMIDPVT
Sbjct: 235 QILSSLVGFICYSRVTLFEALDFRDKNQAEFKCNPEILSCLNPEDFRCPISLELMIDPVT 294

Query: 181 VSTGQTYDRASIQKWLSAGNLLCPKTGERLTSSELVPNTSLKKLIHQFCVDNGISLAKFN 240
           VSTGQTYDRASIQKWLSAGN +CPKTGERLTS ELVPN+SLKKLI+QFC DNGISLAKFN
Sbjct: 295 VSTGQTYDRASIQKWLSAGNFICPKTGERLTSLELVPNSSLKKLINQFCADNGISLAKFN 354

Query: 241 ARSHDITRTIIPGSPAAAEAMKFSSEYLLRRLVFGTDTEKNKAAYEIRLLAKSNIFNRSC 300
           ARSHDITRTIIPGS AAAEA+KF+SE+LLRRLVFGT TEKNKAAYEIRLLAKSNIFNRSC
Sbjct: 355 ARSHDITRTIIPGSLAAAEAIKFTSEFLLRRLVFGTSTEKNKAAYEIRLLAKSNIFNRSC 414

Query: 301 LIKVGIIPSLLNLLSSLDKSTQENAIAAVLKLSKHSTGKILVVENGGLVPILSVLKSGFC 360
           LIK G IP LLNLLSS DKSTQENAIAA+LKLSKHSTGKILV+ENGGL PILSVLKSGFC
Sbjct: 415 LIKAGTIPPLLNLLSSFDKSTQENAIAAILKLSKHSTGKILVMENGGLPPILSVLKSGFC 474

Query: 361 LESRQLAAATLFYLSSVKEYRKLIGETPDAITGLIELIKEGTTCGKKNAVVAIFGLLLYP 420
           LESRQLAAATLFYLSSVKEYRKLIGE PDAITGLI+LIKEGTTCGKKNAVVAIFGLLL P
Sbjct: 475 LESRQLAAATLFYLSSVKEYRKLIGEIPDAITGLIDLIKEGTTCGKKNAVVAIFGLLLCP 534

Query: 421 KNNKTVLNSGVVPILLDIIATSVNDELISDSLAVIAALAESSEGSNTILHASALPLLIRT 480
           KNNKTVLNSG VPILLDIIATS N ELISDSLAVIAALAES+EGSN IL ASALPLLI+T
Sbjct: 535 KNNKTVLNSGAVPILLDIIATSNNSELISDSLAVIAALAESTEGSNAILQASALPLLIKT 594

Query: 481 LNSATSSPGKEYCVSALRSLCNHGGEEVVATLAGDRSIAGSLYSIVTEGSAAAGKKARSL 540
           LNS ++  GKEYCVS LRSLC+HGGEEVVA LAGDR+IAGSLYS+VTEG+AAAGKKARSL
Sbjct: 595 LNSESALAGKEYCVSTLRSLCSHGGEEVVAALAGDRTIAGSLYSVVTEGNAAAGKKARSL 654

Query: 541 LKILHKFRENDVGVNSAVDQERSVRVW 567
           LKILHKFRE+DV   +AVDQERSV VW
Sbjct: 655 LKILHKFREDDVVAYTAVDQERSVDVW 681

BLAST of Cp4.1LG20g02300 vs. ExPASy TrEMBL
Match: A0A5D3BLV3 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G003550 PE=4 SV=1)

HSP 1 Score: 967 bits (2499), Expect = 0.0
Identity = 507/567 (89.42%), Postives = 532/567 (93.83%), Query Frame = 0

Query: 1   MKSQFVATQFWVLIRALATALDVLPLNRIDTSDEVKELVELVVKQARISKFGLDKDDELM 60
           MKSQFVATQFWVLIRALATALDVLPLNRIDTSDEVKELVELV KQARI+KFGLDKDDEL 
Sbjct: 115 MKSQFVATQFWVLIRALATALDVLPLNRIDTSDEVKELVELVAKQARIAKFGLDKDDELT 174

Query: 61  MKRLQSILLQFEKGIEPDLTAIKRVLSYLEIRRWSDCNKEIKFLEEEIDFQYSDLKERDV 120
           MKRLQSILLQFEKGIEPDLTAIKRVL+YLEIRRWSDCNKEIKFLEEEIDFQY DLKERDV
Sbjct: 175 MKRLQSILLQFEKGIEPDLTAIKRVLNYLEIRRWSDCNKEIKFLEEEIDFQYLDLKERDV 234

Query: 121 QILSSLVGFMSYSRLTLFEALDFWDKNQTDIKCNPETLSCLNPQDFRCPISLELMIDPVT 180
           QILSSLVGF+ YSR+TLFEALDF DKNQ + KCNPE LSCLNP+DFRCPISLELMIDPVT
Sbjct: 235 QILSSLVGFICYSRVTLFEALDFRDKNQAEFKCNPEILSCLNPEDFRCPISLELMIDPVT 294

Query: 181 VSTGQTYDRASIQKWLSAGNLLCPKTGERLTSSELVPNTSLKKLIHQFCVDNGISLAKFN 240
           VSTGQTYDRASIQKWLSAGN +CPKTGERLTS ELVPN+SLKKLI+QFC DNGISLAKFN
Sbjct: 295 VSTGQTYDRASIQKWLSAGNFICPKTGERLTSLELVPNSSLKKLINQFCADNGISLAKFN 354

Query: 241 ARSHDITRTIIPGSPAAAEAMKFSSEYLLRRLVFGTDTEKNKAAYEIRLLAKSNIFNRSC 300
           ARSHDITRTIIPGS AAAEA+KF+SE+LLRRLVFGT TEKNKAAYEIRLLAKSNIFNRSC
Sbjct: 355 ARSHDITRTIIPGSLAAAEAIKFTSEFLLRRLVFGTSTEKNKAAYEIRLLAKSNIFNRSC 414

Query: 301 LIKVGIIPSLLNLLSSLDKSTQENAIAAVLKLSKHSTGKILVVENGGLVPILSVLKSGFC 360
           LIK G IP LLNLLSS DKSTQENAIAA+LKLSKHSTGKILV+ENGGL PILSVLKSGFC
Sbjct: 415 LIKAGTIPPLLNLLSSFDKSTQENAIAAILKLSKHSTGKILVMENGGLPPILSVLKSGFC 474

Query: 361 LESRQLAAATLFYLSSVKEYRKLIGETPDAITGLIELIKEGTTCGKKNAVVAIFGLLLYP 420
           LESRQLAAATLFYLSSVKEYRKLIGE PDAITGLI+LIKEGTTCGKKNAVVAIFGLLL P
Sbjct: 475 LESRQLAAATLFYLSSVKEYRKLIGEIPDAITGLIDLIKEGTTCGKKNAVVAIFGLLLCP 534

Query: 421 KNNKTVLNSGVVPILLDIIATSVNDELISDSLAVIAALAESSEGSNTILHASALPLLIRT 480
           KNNKTVLNSG VPILLDIIATS N ELISDSLAVIAALAES+EGSN IL ASALPLLI+T
Sbjct: 535 KNNKTVLNSGAVPILLDIIATSNNSELISDSLAVIAALAESTEGSNAILQASALPLLIKT 594

Query: 481 LNSATSSPGKEYCVSALRSLCNHGGEEVVATLAGDRSIAGSLYSIVTEGSAAAGKKARSL 540
           LNS ++  GKEYCVS LRSLC+HGGEEV A LAGDR+IAGSLYS+VTEG+AAAGKKARSL
Sbjct: 595 LNSESALAGKEYCVSTLRSLCSHGGEEVAAALAGDRTIAGSLYSVVTEGNAAAGKKARSL 654

Query: 541 LKILHKFRENDVGVNSAVDQERSVRVW 567
           LKILHKFRE+DV   +AVDQERSV VW
Sbjct: 655 LKILHKFREDDVVAYTAVDQERSVDVW 681

BLAST of Cp4.1LG20g02300 vs. ExPASy TrEMBL
Match: A0A1S3AVW8 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103483402 PE=4 SV=1)

HSP 1 Score: 967 bits (2499), Expect = 0.0
Identity = 507/567 (89.42%), Postives = 532/567 (93.83%), Query Frame = 0

Query: 1   MKSQFVATQFWVLIRALATALDVLPLNRIDTSDEVKELVELVVKQARISKFGLDKDDELM 60
           MKSQFVATQFWVLIRALATALDVLPLNRIDTSDEVKELVELV KQARI+KFGLDKDDEL 
Sbjct: 115 MKSQFVATQFWVLIRALATALDVLPLNRIDTSDEVKELVELVAKQARIAKFGLDKDDELT 174

Query: 61  MKRLQSILLQFEKGIEPDLTAIKRVLSYLEIRRWSDCNKEIKFLEEEIDFQYSDLKERDV 120
           MKRLQSILLQFEKGIEPDLTAIKRVL+YLEIRRWSDCNKEIKFLEEEIDFQY DLKERDV
Sbjct: 175 MKRLQSILLQFEKGIEPDLTAIKRVLNYLEIRRWSDCNKEIKFLEEEIDFQYLDLKERDV 234

Query: 121 QILSSLVGFMSYSRLTLFEALDFWDKNQTDIKCNPETLSCLNPQDFRCPISLELMIDPVT 180
           QILSSLVGF+ YSR+TLFEALDF DKNQ + KCNPE LSCLNP+DFRCPISLELMIDPVT
Sbjct: 235 QILSSLVGFICYSRVTLFEALDFRDKNQAEFKCNPEILSCLNPEDFRCPISLELMIDPVT 294

Query: 181 VSTGQTYDRASIQKWLSAGNLLCPKTGERLTSSELVPNTSLKKLIHQFCVDNGISLAKFN 240
           VSTGQTYDRASIQKWLSAGN +CPKTGERLTS ELVPN+SLKKLI+QFC DNGISLAKFN
Sbjct: 295 VSTGQTYDRASIQKWLSAGNFICPKTGERLTSLELVPNSSLKKLINQFCADNGISLAKFN 354

Query: 241 ARSHDITRTIIPGSPAAAEAMKFSSEYLLRRLVFGTDTEKNKAAYEIRLLAKSNIFNRSC 300
           ARSHDITRTIIPGS AAAEA+KF+SE+LLRRLVFGT TEKNKAAYEIRLLAKSNIFNRSC
Sbjct: 355 ARSHDITRTIIPGSLAAAEAIKFTSEFLLRRLVFGTSTEKNKAAYEIRLLAKSNIFNRSC 414

Query: 301 LIKVGIIPSLLNLLSSLDKSTQENAIAAVLKLSKHSTGKILVVENGGLVPILSVLKSGFC 360
           LIK G IP LLNLLSS DKSTQENAIAA+LKLSKHSTGKILV+ENGGL PILSVLKSGFC
Sbjct: 415 LIKAGTIPPLLNLLSSFDKSTQENAIAAILKLSKHSTGKILVMENGGLPPILSVLKSGFC 474

Query: 361 LESRQLAAATLFYLSSVKEYRKLIGETPDAITGLIELIKEGTTCGKKNAVVAIFGLLLYP 420
           LESRQLAAATLFYLSSVKEYRKLIGE PDAITGLI+LIKEGTTCGKKNAVVAIFGLLL P
Sbjct: 475 LESRQLAAATLFYLSSVKEYRKLIGEIPDAITGLIDLIKEGTTCGKKNAVVAIFGLLLCP 534

Query: 421 KNNKTVLNSGVVPILLDIIATSVNDELISDSLAVIAALAESSEGSNTILHASALPLLIRT 480
           KNNKTVLNSG VPILLDIIATS N ELISDSLAVIAALAES+EGSN IL ASALPLLI+T
Sbjct: 535 KNNKTVLNSGAVPILLDIIATSNNSELISDSLAVIAALAESTEGSNAILQASALPLLIKT 594

Query: 481 LNSATSSPGKEYCVSALRSLCNHGGEEVVATLAGDRSIAGSLYSIVTEGSAAAGKKARSL 540
           LNS ++  GKEYCVS LRSLC+HGGEEV A LAGDR+IAGSLYS+VTEG+AAAGKKARSL
Sbjct: 595 LNSESALAGKEYCVSTLRSLCSHGGEEVAAALAGDRTIAGSLYSVVTEGNAAAGKKARSL 654

Query: 541 LKILHKFRENDVGVNSAVDQERSVRVW 567
           LKILHKFRE+DV   +AVDQERSV VW
Sbjct: 655 LKILHKFREDDVVAYTAVDQERSVDVW 681

BLAST of Cp4.1LG20g02300 vs. TAIR 10
Match: AT1G60190.1 (ARM repeat superfamily protein )

HSP 1 Score: 401.7 bits (1031), Expect = 9.5e-112
Identity = 228/575 (39.65%), Postives = 354/575 (61.57%), Query Frame = 0

Query: 1   MKSQFVATQFWVLIRALATALDVLPLNRIDTSDEVKELVELVVKQARISKFGLDKDDELM 60
           M S  V+  F  L R+++T+LD  P+  +D   EV EL+ LV++Q R S+   D+DD+  
Sbjct: 119 MNSGQVSAHFRDLTRSISTSLDTFPVRSVDLPGEVNELIYLVMRQTRKSEARPDRDDKRA 178

Query: 61  MKRLQSILLQFEKGIEPDLTAIKRVLSYLEIRRWSDCNKEIKFLEEEIDFQYSDLKERDV 120
           +  +      FE  I P+   I RVL ++ +R+W DC KEI FL EEI          ++
Sbjct: 179 IDSVYWFFNLFENRINPNSDEILRVLDHIGVRKWRDCVKEIDFLREEI--SVGKKSNIEI 238

Query: 121 QILSSLVGFMSYSRLTLFEALDFWDKNQTDIKCNPETLSCLNPQDFRCPISLELMIDPVT 180
           ++LS+L+GF+ Y R  +   +D  D+ +   + +   +  LN  D RCPISLE+M DPV 
Sbjct: 239 ELLSNLMGFICYCRCVILRGIDVDDEEKDKEEDDLMMVRSLNVDDLRCPISLEIMSDPVV 298

Query: 181 VSTGQTYDRASIQKWLSAGNLLCPKTGERLTSSELVPNTSLKKLIHQFCVDNGISLAKFN 240
           + +G TYDR+SI KW ++GN+ CPKTG+ L S+ LV N S+K++I  +   NG+ + +  
Sbjct: 299 LESGHTYDRSSITKWFASGNITCPKTGKTLVSTVLVDNFSVKQVIQSYSKQNGVVMGQKG 358

Query: 241 ARSHDITRTIIPGSPAAAEAMKFSSEYLLRRLVFGTDTEKNKAAYEIRLLAKSNIFNRSC 300
            +  D+  ++     AA EA K ++E+L   L+ G + E  KA  EIR+L K++ F RSC
Sbjct: 359 KKKVDVAESL-----AAEEAGKLTAEFLAGELIKGDEEEMVKALVEIRILTKTSTFYRSC 418

Query: 301 LIKVGIIPSLLNLLSSLDKSTQENAIAAVLKLSKHSTGKILVV--ENGGLVPILSVLKSG 360
           L++ G++ SL+ +L S D   QENA+A ++ LSK   GK  +V  + GGL  I+ VL  G
Sbjct: 419 LVEAGVVESLMKILRSDDPRIQENAMAGIMNLSKDIAGKTRIVGEDGGGLRLIVEVLNDG 478

Query: 361 FCLESRQLAAATLFYLSSVKEYRKLIGETPDAITGLIELIK--EGTTCGKKNAVVAIFGL 420
              ESRQ AAA LFYLSS+ +Y +LIGE  DAI GL+ ++K  +     K+NA++AI  L
Sbjct: 479 ARRESRQYAAAALFYLSSLGDYSRLIGEISDAIPGLVRIVKSCDYGDSAKRNALIAIRSL 538

Query: 421 LL-YPKNNKTVLNSGVVPILLDII-ATSVNDELISDSLAVIAALAESSEGSNTILHASAL 480
           L+  P N+  +L +G+VP+LLD++ +  ++D + +DS+A++A +AE  +G  ++L    L
Sbjct: 539 LMNQPDNHWRILAAGIVPVLLDLVKSEEISDGVTADSMAILAKMAEYPDGMISVLRRGGL 598

Query: 481 PLLIRTLNSATSSPG-KEYCVSALRSLCNHGGEEVVATLAGDRSIAGSLYSIVTEGSAAA 540
            L ++ L S+  SP  K++CV+ L +LC++GG +VV +LA + SI GSLY+  + G    
Sbjct: 599 KLAVKILGSSEVSPATKQHCVALLLNLCHNGGSDVVGSLAKNPSIMGSLYTASSNGELGG 658

Query: 541 GKKARSLLKILHKFRENDVGVNSAV-DQERSVRVW 568
           GKKA +L+K++H+F+E   G    V ++ER V  W
Sbjct: 659 GKKASALIKMIHEFQERKTGPGEPVLERERFVHAW 686

BLAST of Cp4.1LG20g02300 vs. TAIR 10
Match: AT1G10560.1 (plant U-box 18 )

HSP 1 Score: 392.9 bits (1008), Expect = 4.4e-109
Identity = 234/584 (40.07%), Postives = 358/584 (61.30%), Query Frame = 0

Query: 1   MKSQFVATQFWVLIRALATALDVLPLNRIDTSDEVKELVELVVKQARISKFGL--DKDDE 60
           M S  V+    VL R+++T+L   P+  +D + EV EL++LVV+QAR  K+G+  + +D+
Sbjct: 120 MNSDQVSDHLRVLTRSISTSLSAFPVASVDLTTEVNELIDLVVRQAR--KYGVQPETNDK 179

Query: 61  LMMKRLQSILLQFEKGIEPDLTAIKRVLSYLEIRRWSDCNKEIKFLEEEIDFQYSDLKER 120
             +  +  IL  F   + PD   I R+L ++ IR+W DC KEI FL EEID +  D K++
Sbjct: 180 RAVSSINRILALFVNRVVPDPDEINRILDHVGIRKWGDCVKEINFLGEEIDAERLDEKKK 239

Query: 121 ----DVQILSSLVGFMSYSR---LTLFEALDFWDKNQTDIKCNPETLSCLNPQDFRCPIS 180
                V++LSSL+GF+ Y R   L   E  D  + ++  IK + + +  L  +D  CPIS
Sbjct: 240 KSSDQVELLSSLMGFICYCRCIILGRIERDDHHNHHEDGIKKDHDLIRGLKVEDLLCPIS 299

Query: 181 LELMIDPVTVSTGQTYDRASIQKWLSAGNLLCPKTGERLTSSELVPNTSLKKLIHQFCVD 240
           LE+M DPV + TG TYDR+SI KW  +GN+ CP TG+ LTS+ELV N S++++I + C  
Sbjct: 300 LEIMTDPVVIETGHTYDRSSITKWFGSGNITCPITGKILTSTELVDNVSVRQVIRKHCKT 359

Query: 241 NGISLAKFNAR--SHDITRTIIPGSPAAAEAMKFSSEYLLRRLVFGTDTEKNKAAYEIRL 300
           NGI LA  + R  SHD    ++P S AA  A K  +++L   L+ G +    +A  EIR+
Sbjct: 360 NGIVLAGISRRRKSHD---DVVPESLAAKGAGKLIAKFLTSELINGGEEMIYRAVREIRV 419

Query: 301 LAKSNIFNRSCLIKVGIIPSLLNLLSSLDKSTQENAIAAVLKLSKHSTGKILVVENGGLV 360
             K++ FNRSCL+K G +  LL LLSS+D   QENA+A +L LSKH TGK  +    GL 
Sbjct: 420 QTKTSSFNRSCLVKAGAVTPLLKLLSSVDIRIQENAMAGILNLSKHVTGKSKIA-GEGLK 479

Query: 361 PILSVLKSGFCLESRQLAAATLFYLSSVKEYRKLIGETPDAITGLIELIK--EGTTCGKK 420
            ++ +L  G   E+R  +A+ LFYLSSV++Y +LIGE PDAI GL+ ++K  +     K+
Sbjct: 480 ILVEILNEGAKTETRLYSASALFYLSSVEDYSRLIGENPDAIPGLMNIVKGDDYGDSAKR 539

Query: 421 NAVVAIFGLLLYPKNNKTVLNSGVVPILLDIIAT-SVNDELISDSLAVIAALAESSEGSN 480
           +A++A+ GLL+   N+  VL +G VPILLD++ +  ++  L +D LA +A LAE  +G+ 
Sbjct: 540 SALLAVMGLLMQSDNHWRVLAAGAVPILLDLLRSGEISGGLTADCLATLAKLAEYPDGTI 599

Query: 481 TILHASALPLLIRTLNSATSSP--GKEYCVSALRSLCNHGGEEVVATLAGDRSIAGSLYS 540
            ++    L L ++ L+S+  SP   K++CV  + +LC +GG +VV  L  +  + GSLY+
Sbjct: 600 GVIRRGGLKLAVKILSSSEDSPVAVKQHCVGLILNLCLNGGRDVVGVLVKNSLVMGSLYT 659

Query: 541 IVTEGSAAAGKKARSLLKILHKFRENDVG-VNSAVDQERSVRVW 568
           +++ G     KKA +L++++H+F+E   G V   + + R V  W
Sbjct: 660 VLSNGEYGGSKKASALIRMIHEFQERKTGSVEPNLQRGRFVHAW 697

BLAST of Cp4.1LG20g02300 vs. TAIR 10
Match: AT1G29340.1 (plant U-box 17 )

HSP 1 Score: 290.8 bits (743), Expect = 2.4e-78
Identity = 200/564 (35.46%), Postives = 310/564 (54.96%), Query Frame = 0

Query: 1   MKSQFVATQFWVLIRALATALDVLPLNRIDTSDEVKELVELVVKQARISKFGLDKDDELM 60
           +++  ++  F  L + ++T LDVLP+N +  SD+++E +EL+ +Q+R ++  +DK+DE +
Sbjct: 134 LQNPSISGYFHDLNQEISTLLDVLPVNDLGLSDDIREQIELLQRQSRKARLYIDKNDESL 193

Query: 61  MKRLQSILLQFEKGIEPDLTAIKR-VLSYLEIRRWSDCNKEIKFLEEEIDFQYSDLKERD 120
            +   S L  FE G  P    ++   +  L IR    C  EI+FLEE+I     DL E  
Sbjct: 194 RESFYSFLDGFENGKIPSSVDLRMFFVEKLGIRDSKSCRSEIEFLEEQIVNHDGDL-EPT 253

Query: 121 VQILSSLVGFMSYSRLTLF----EALDFWDKNQTD------IKCNPETLSCLNPQDFRCP 180
             +++  V    Y R  LF    + +++W +N         +           P+DF CP
Sbjct: 254 GSVINGFVAITRYCRFLLFGFEEDGMEWWIENNPKKPRKGFVAQEIGDTFITVPKDFVCP 313

Query: 181 ISLELMIDPVTVSTGQTYDRASIQKWLSAGNLLCPKTGERLTSSELVPNTSLKKLIHQFC 240
           ISL+LM DPV +STGQTYDR SI +W+  G+  CPKTG+ L  S +VPN +LK LI Q+C
Sbjct: 314 ISLDLMTDPVIISTGQTYDRNSIARWIEEGHCTCPKTGQMLMDSRIVPNRALKNLIVQWC 373

Query: 241 VDNGISL-AKFNARSHDITRTIIPGSPAAAEAMKFSSEYLLRRLVFGTDTEKNKAAYEIR 300
             +GIS  ++F    ++   + +P + AA EA K +   L++ L  G+   +  AA EIR
Sbjct: 374 TASGISYESEFTDSPNESFASALP-TKAAVEANKATVSILIKYLADGSQAAQTVAAREIR 433

Query: 301 LLAKSNIFNRSCLIKVGIIPSLLNLLSSLDKSTQENAIAAVLKLSKHSTGKILVVENGG- 360
           LLAK+   NR+ + + G IP L  LL+S +   QEN++ A+L LS +   K  ++E G  
Sbjct: 434 LLAKTGKENRAYIAEAGAIPHLCRLLTSENAIAQENSVTAMLNLSIYEKNKSRIMEEGDC 493

Query: 361 LVPILSVLKSGFCLESRQLAAATLFYLSSVKEYRKLIGETPDAITGLIELIKEGTTCGKK 420
           L  I+SVL SG  +E+++ AAATLF LS+V EY+K I      +  L  L++ GT  GKK
Sbjct: 494 LESIVSVLVSGLTVEAQENAAATLFSLSAVHEYKKRIAIVDQCVEALALLLQNGTPRGKK 553

Query: 421 NAVVAIFGLLLYPKNNKTVLNSGVVPILLDIIATSVNDELISDSLAVIAALAESSEGSNT 480
           +AV A++ L  +P N   ++  G V  L+  +    N+ +  ++   +A L   S G+  
Sbjct: 554 DAVTALYNLSTHPDNCSRMIEGGGVSSLVGALK---NEGVAEEAAGALALLVRQSLGAEA 613

Query: 481 I-LHASALPLLIRTLNSATSSPGKEYCVSALRSLCNHGGEEVVATLAGDRSIAGSLYSIV 540
           I    SA+  L+  +   T   GKE  V+AL  LC  GG  V   +    +IAG L +++
Sbjct: 614 IGKEDSAVAGLMGMMRCGTPR-GKENAVAALLELCRSGGAAVAEKVLRAPAIAGLLQTLL 673

Query: 541 TEGSAAAGKKARSLLKILHKFREN 551
             G+  A +KA SL ++  + REN
Sbjct: 674 FTGTKRARRKAASLARVFQR-REN 690

BLAST of Cp4.1LG20g02300 vs. TAIR 10
Match: AT5G01830.1 (ARM repeat superfamily protein )

HSP 1 Score: 260.4 bits (664), Expect = 3.4e-69
Identity = 179/565 (31.68%), Postives = 290/565 (51.33%), Query Frame = 0

Query: 1   MKSQFVATQFWVLIRALATALDVLPLNRIDTSDEVKELVELVVKQARISKFGLDKDDELM 60
           ++   VA  F  L+  L+T LD+LPL+  D SD+ ++L+ L+ KQ   S   +D  D  +
Sbjct: 120 LQIDIVAFNFHELVTDLSTVLDILPLHDFDLSDDAQDLISLLTKQCSDSVQFVDARDVAL 179

Query: 61  MKRLQSILLQFEKGIEPDLTAIKRVLSYLEIRRWSDCNKEIKFLEEEIDFQYSDLKERDV 120
            +++   +   +  I PD + + ++ + L +   +    EI+ LE+EI  Q  D   R  
Sbjct: 180 RRKVTDTIAGIKHQISPDHSTLIKIFNDLGLSDSASLTDEIQRLEDEIQDQIDD---RSK 239

Query: 121 QILSSLVGFMSYSRLTLFEALDFWDKNQTDIKCNPETLSCLNPQDFRCPISLELMIDPVT 180
              +SL+G + YS+  L+           D + +        P DFRCPI+LELM DPV 
Sbjct: 240 SAAASLIGLVRYSKCVLYGP----STPAPDFRRHQSLSDANIPADFRCPITLELMRDPVV 299

Query: 181 VSTGQTYDRASIQKWLSAGNLLCPKTGERLTSSELVPNTSLKKLIHQFCVDNGISLAKFN 240
           V+TGQTYDR SI  W+ +G+  CPKTG+ L  + LVPN +LK LI  +C D  I    + 
Sbjct: 300 VATGQTYDRESIDLWIQSGHNTCPKTGQVLKHTSLVPNRALKNLIVLWCRDQKIPFELYG 359

Query: 241 ARSHDITRTIIPGSPA----AAEAMKFSSEYLLRRLVFGTDTEKNKAAYEIRLLAKSNIF 300
                       G PA    A E  K    +L+ +L   +  + N   +E+R LAKS+  
Sbjct: 360 DGG---------GEPAPCKEAVEFTKMMVSFLIEKL---SVADSNGVVFELRALAKSDTV 419

Query: 301 NRSCLIKVGIIPSLLNLLSSLDKSTQENAIAAVLKLSKHSTGKILVVE-NGGLVPILSVL 360
            R+C+ + G IP L+  L++   S Q NA+  +L LS     K  ++E +G L  ++ VL
Sbjct: 420 ARACIAEAGAIPKLVRYLATECPSLQINAVTTILNLSILEQNKTRIMETDGALNGVIEVL 479

Query: 361 KSGFCLESRQLAAATLFYLSSVKEYRKLIGETPDAITGLIELIKEGTTCGKKNAVVAIFG 420
           +SG   E++  AAATLF L+ V  YR+ +G     ++GL++L K+G T  K++A+VAI  
Sbjct: 480 RSGATWEAKANAAATLFSLAGVSAYRRRLGRKARVVSGLVDLAKQGPTSSKRDALVAILN 539

Query: 421 LLLYPKNNKTVLNSGVVPILLDIIATSVNDELISDSLAVIAALAESSEGSNTILHASALP 480
           L+   +N    + +GV+    D        EL  +++AV+ A+     G   +  A +L 
Sbjct: 540 LVAERENVGRFVEAGVMGAAGDAF-----QELPEEAVAVVEAVVRRG-GLMAVSAAFSLI 599

Query: 481 LLIRTLNSATSSPGKEYCVSALRSLCNHGGEEVVATLAGDRSIAGSLYSIVTEGSAAAGK 540
            L+  +    +   +E   + L ++C  GG E+VA +A    I   ++ ++  G+A  G+
Sbjct: 600 RLLGEVMREGADTTRESAAATLVTMCRKGGSELVAEMAAIPGIERVIWEMIGAGTARGGR 659

Query: 541 KARSLLKILHKFRENDVGVNSAVDQ 561
           KA SL++ L ++   D    +A  Q
Sbjct: 660 KAASLMRYLRRWAAGDTHNTAAETQ 659

BLAST of Cp4.1LG20g02300 vs. TAIR 10
Match: AT3G54850.1 (plant U-box 14 )

HSP 1 Score: 258.1 bits (658), Expect = 1.7e-68
Identity = 188/534 (35.21%), Postives = 287/534 (53.75%), Query Frame = 0

Query: 17  LATALDVLPLNRIDTSDEVKELVELVVKQARISKFGLDKDDELMMKRLQSILLQFEKGIE 76
           +  AL  +P  +I+ S+EV+E V+L+  Q + +K   ++ D     +L   L   E  ++
Sbjct: 112 IEAALSQIPYEKIEVSEEVREQVQLLHFQFKRAKERWEESD----LQLSHDLAMAENVMD 171

Query: 77  PDLTAIKRVLSYLEIRRWSDCNKEIKFLEE---EIDFQYSDLKERDVQILSSLVGFMSYS 136
           PD   +KR+   L++    +  KE   + E     D    D  ER   +L +LV F++  
Sbjct: 172 PDPIILKRLSQELQLTTIDELKKESHAIHEYFLSYDGDPDDCFERMSSLLKNLVDFVT-- 231

Query: 137 RLTLFEALDFWDKNQTDIKCNPETLSCLNPQDFRCPISLELMIDPVTVSTGQTYDRASIQ 196
                E+ D      T  +      S + P+ FRCPISLELM DPV VSTGQTY+R+SIQ
Sbjct: 232 ----MESSD--PDPSTGSRIVSRHRSPVIPEYFRCPISLELMKDPVIVSTGQTYERSSIQ 291

Query: 197 KWLSAGNLLCPKTGERLTSSELVPNTSLKKLIHQFCVDNGISLAKFNARSHDITRTIIPG 256
           KWL AG+  CPK+ E L  + L PN  LK LI  +C  NGI L     ++    RT   G
Sbjct: 292 KWLDAGHKTCPKSQETLLHAGLTPNYVLKSLIALWCESNGIEL----PQNQGSCRTTKIG 351

Query: 257 SPAAAEAMKFSSEYLLRRLVFGTDTEKNKAAYEIRLLAKSNIFNRSCLIKVGIIPSLLNL 316
             ++++  +     LL +L  GT  ++  AA E+RLLAK N+ NR C+ + G IP L+ L
Sbjct: 352 GSSSSDCDRTFVLSLLEKLANGTTEQQRAAAGELRLLAKRNVDNRVCIAEAGAIPLLVEL 411

Query: 317 LSSLDKSTQENAIAAVLKLSKHSTGKILVVENGGLVPILSVLKSGFCLESRQLAAATLFY 376
           LSS D  TQE+++ A+L LS +   K  +V+ G +  I+ VLK+G  +E+R+ AAATLF 
Sbjct: 412 LSSPDPRTQEHSVTALLNLSINEGNKGAIVDAGAITDIVEVLKNG-SMEARENAAATLFS 471

Query: 377 LSSVKEYRKLIGETPDAITGLIELIKEGTTCGKKNAVVAIFGLLLYPKNNKTVLNSGVVP 436
           LS + E +  IG    AI  LI L++EGT  GKK+A  AIF L +Y  N    +  G+V 
Sbjct: 472 LSVIDENKVAIG-AAGAIQALISLLEEGTRRGKKDAATAIFNLCIYQGNKSRAVKGGIVD 531

Query: 437 ILLDIIATSVNDELISDSLAVIAALAESSEGSNTILHASALPLLIRTLNSATSSP-GKEY 496
            L  ++       ++ ++LA++A L+ + EG   I  A ++P+L+  +   T SP  +E 
Sbjct: 532 PLTRLL-KDAGGGMVDEALAILAILSTNQEGKTAIAEAESIPVLVEIIR--TGSPRNREN 591

Query: 497 CVSALRSLCNHGGEEVVATLAGDRSIAGSLYSIVTEGSAAAGKKARSLLKILHK 547
             + L  LC    E +   +A +     +L  +   G+  A +KA SLL+++ +
Sbjct: 592 AAAILWYLCIGNIERL--NVAREVGADVALKELTENGTDRAKRKAASLLELIQQ 622

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O807421.3e-11039.65U-box domain-containing protein 19 OS=Arabidopsis thaliana OX=3702 GN=PUB19 PE=2... [more]
Q9XIJ56.2e-10840.07U-box domain-containing protein 18 OS=Arabidopsis thaliana OX=3702 GN=PUB18 PE=1... [more]
E4NKF83.9e-7833.22U-box domain-containing protein 1 OS=Medicago truncatula OX=3880 GN=PUB1 PE=1 SV... [more]
Q9C7R63.3e-7735.46U-box domain-containing protein 17 OS=Arabidopsis thaliana OX=3702 GN=PUB17 PE=2... [more]
Q9LZW34.8e-6831.68U-box domain-containing protein 16 OS=Arabidopsis thaliana OX=3702 GN=PUB16 PE=2... [more]
Match NameE-valueIdentityDescription
XP_023519714.10.0100.00U-box domain-containing protein 19-like [Cucurbita pepo subsp. pepo][more]
KAG7019826.10.098.77U-box domain-containing protein 19, partial [Cucurbita argyrosperma subsp. argyr... [more]
XP_022923709.10.097.71LOW QUALITY PROTEIN: U-box domain-containing protein 19-like [Cucurbita moschata... [more]
XP_023000658.10.097.88U-box domain-containing protein 19-like [Cucurbita maxima][more]
KAG6584229.10.098.40U-box domain-containing protein 19, partial [Cucurbita argyrosperma subsp. soror... [more]
Match NameE-valueIdentityDescription
A0A6J1E7620.097.71RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111431336... [more]
A0A6J1KN840.097.88RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111495027 P... [more]
A0A5A7UP250.089.59RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A5D3BLV30.089.42RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3AVW80.089.42RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103483402 PE=4 ... [more]
Match NameE-valueIdentityDescription
AT1G60190.19.5e-11239.65ARM repeat superfamily protein [more]
AT1G10560.14.4e-10940.07plant U-box 18 [more]
AT1G29340.12.4e-7835.46plant U-box 17 [more]
AT5G01830.13.4e-6931.68ARM repeat superfamily protein [more]
AT3G54850.11.7e-6835.21plant U-box 14 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000225ArmadilloSMARTSM00185arm_5coord: 461..502
e-value: 9.9
score: 13.3
coord: 335..376
e-value: 13.0
score: 12.3
coord: 419..460
e-value: 15.0
score: 11.8
coord: 293..334
e-value: 1.0
score: 18.4
IPR000225ArmadilloPFAMPF00514Armcoord: 297..330
e-value: 5.5E-6
score: 26.2
IPR000225ArmadilloPROSITEPS50176ARM_REPEATcoord: 305..347
score: 9.7824
IPR003613U box domainSMARTSM00504Ubox_2coord: 165..228
e-value: 2.8E-27
score: 106.6
IPR003613U box domainPFAMPF04564U-boxcoord: 163..231
e-value: 1.7E-18
score: 66.6
IPR003613U box domainPROSITEPS51698U_BOXcoord: 161..235
score: 36.49028
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 259..415
e-value: 4.2E-20
score: 74.2
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 416..562
e-value: 1.0E-9
score: 40.0
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 162..236
e-value: 1.2E-25
score: 91.4
NoneNo IPR availablePANTHERPTHR23315:SF116OS07G0532750 PROTEINcoord: 1..551
NoneNo IPR availablePANTHERPTHR23315U BOX DOMAIN-CONTAININGcoord: 1..551
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 166..208
e-value: 2.73467E-25
score: 96.1486
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 163..235
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 269..529

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG20g02300.1Cp4.1LG20g02300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005515 protein binding
molecular_function GO:0004842 ubiquitin-protein transferase activity