Cp4.1LG04g01360 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG04g01360
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionProcollagen-proline 4-dioxygenase
LocationCp4.1LG04: 777969 .. 781994 (+)
RNA-Seq ExpressionCp4.1LG04g01360
SyntenyCp4.1LG04g01360
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTGTAAGCAGCGGTTTCGGAGCAGATCCTCTTCTGGGCTGCCCATTAATTCTCCTCTTAAATGAGTATGAACCAGAAAATTCGAAGCGATCGCTTTCTCAGTCATCCATTTTCGTTCTTTGATTTGATTTGATTTCTCGTTCTTTTCCATTTATTCGTTTATATAATTCGCTTTTTTTCTCTTTAATTTCTGAGTTTCAGAGCTCGAGTTTCGCCATGGATTCTCGATTTTTTCTTGCATTTTCTCTTTGTTTCCTCTGTTCATTCCCTCTATTTGCTCGCTCCGCCAATCGATTGCCGAAATTACTCTTGGACGACACGAAAACGTAATAATACTTCATTGATTTCTCTGTTTTTGTTTATCTCCGTTCATCCGATATTTGAAATTTTGACCTAATTGATATGATTTTCACAGGGAAGATTCTGTTATTAGGATGAAAATGGACGGTTCCTCCATTAAAATCGATCCCACTCGCGTCGTTCAGCTTTCATCGCAACCTAGGTCCGTAATGTTCTTAATTTTTTCTATGAATTTTCCTGATGAAGTCTAGAATCTTGCACATTTTCGGCTATTTGTTTCCATTTCTTTCTTTTTTGCTCAATTAGATGAACATATACAGGGCTTTCTTATACAAGGGATTTTTGTCTGCAGAGGAGTGCCAGCATCTTATCGATTTGGTATGGTGAATTTCTCTTTGTTCATTTTCCTTTTCCTGTTTGGAGGCTGAGAAAAATTGATCCTCGGACTTAATTGTGTATAATTGTGAAATCTCACATCGATTGTTGCGGAGGAGAATGAAACATTCTTTACAAGAGTTTAGAAACCTCTCCCTAGTCTTTTATAAGAGTGTAGAAACTTCTCTTAAGTAGACACGTTTTAAAAACCTTGAGGGAAAGCCTGAAAGAGAGCTAGCTCAAAGATGACAATATCTGCTAGTGGTGGGCTTGGGCTGTTACAAATGGTATCAGAGCCAGACACCGGATAGTGTGCCAATGAGGATGGTAGTGTGCTAGTGAGGATGTTGGGCTTCGAAGGGGGTGGATTGTGAGATCCTACATCGACTGAGAGGGGAATGACATCGATTGGAGAGGAGAATGAGTGTTAGCGAGAATGCTGGGCTCTGAAGGGGATGGATTGTGAGATCCCATGTCGGTTGGGGCAGAGTACAGAACATTATTTATATAAGAGTGTAAAAACTTCTCCCTAGCAAACGTGTTTTAAAAACCTTGAGGGGAAGTCTGAAAGGGAAAGCCCAAATAGGACGATATTTGCTAGCGGTTGGCTTGGGCTGTTACAAATGGTGTTAGAGCCAGACACCGAGCAATGTGCTAGCAAGGACGTTGGGCCCCGAAGGGGTGTGGATTGTGAGATCCTATATCGATTGGAGAGGGGAACAACATCGATTGGAGAGGAGAACGAGTGTCAGCGAGGATGCTGGGCTCTGAAGGGGATGGATTGTGAGATCCCATGTCGGTTGGGGCAGAGTACAGAACATTATTTATATAAGAGTGTAAAAACTTCTCCCTAGCAAACGTGTTTTAAAAACCTTGAGGGGAAGTCTGAAAGGGAAAGCCCAAATAGTGATATTTGCTAGCGGTTGGCTTGGGCTGTTACAAATGGTGTTAGAGCCAGACACCGAGCAATGTGCTAGCAAGGACGTTGGGCCCCGAAGGGGTGTGGATTGCGAGATCCTATATCGATTGGAGAGGGGAACAACATCGATTGGAGAGGAGAACGAGTGCCAGCAAGGACGCTGGCCCCTGAAGGGTATGGATTGTAATATCCCACATCGGTTGGGGAGTAGAACAAAACATTCTTTATAAGAGTGCAGAAACCTCTCTCTAACAGACACATTTTAAAAATCTTGAGGGAAAGCCTAAAGAGGACAATATTTGCTAGCGATGGACTTGAGCTGTTACAAATGGTATTAAAGCCAGACACCGGGTAATGTGCCAGCAAGGATGTTACGCCCACATCGACCAGAGAGGGAAACGACATCGATTGGAGAGGAGAACGAGTACAGGCGAGGATGTTGGGCCCTGAAGAGGGATGGATTGTGAGATCCCACATCGGTTGGGGAGGAGAACGAAGCATTTCTAATAAGAGTGAGCGAAAGCATTTTAAAAACCTCAAGGGGAAGCCCAAAGAGGACAATATTTACTAGTAGTGGACTTGGGCTGATACAATAACTGTCATATACTTTTTGTAGGCGAAGGATTACTTAGAGCAATCATTGGTGGTCGATGACATTACGGGTGCAAGTCGTTCGAGTACTGACCGGACGAGTACCGGCATGTTTCTTTACAAGGCTCAGGTATTTGACTCCAGTATTAACAAAACATCTGCCAGTTTCTGCAGTTTAGATTCCCTTTTCCAATACTTTTCTCAAATGGGCTTCATAAAATTCATGAAAATTTGATTTAGTGTTCATCTAGATTGGCCTAAGTTTGATGTGGTCTACTATAAAAACTATCAGTAAGAACAGATTGTTCAAATTTATGCATTAAAGAAGAACAAGGACGGCCTTGACACAGATATAATACTACAACTGTGGCATAGTTGCTAACATTGAGTCCCCTTCCCCTCCCCAGGATGACATAGTTGCTGGCATTGAGGCCAAGATTGCTGCGTGGACGTTCCTTCCCGTCGGTAAACGACTTATAGATCTTTTGATGTTGTATCGTTTATTTATTTACAGAATGAGGCTCGTAAAATCTCGAAATTGCTCTTTCGGATCAGATAATGGGGAGCCTATACAAATACTAAGGTATGAAAATGGTCAGCAATATGTACCACATTTTGATTTTTTTCAAGATCCAGTCAATGTAGCTGCTGGTGGTCATCGGATAGCCACAGTCTTGATGTATTTGTCCAATGTTGAAAGGGGTGGAGAAACTGTCTTTCCCGATTCTCCGGTATTACTTTCTTCGAACGCTAACTGCTCTCGTTCGGACTTTTCATAATTCGTTTCGAGTTGAAGTTTGGAACTTGTTTATGAAGTGTTGCAATTTATAATCTCTCTTTGACAGGCTAAAGTATTCGAGGAGGAGAACAAGGATTTGTCCGATTGCTCTACGACCGGTTATGGAGGTATTGAGTTTTTTTCTTCAAATTTTTTTATCACGGCTAAGTTAAAAGCATGATTTTGATACCATGTTAGGACAGCCCAAGCCCACTGCTAGCAAATATTGTCCTCTTTGAGTTTATCCTTTCATACTCTCTCAAGGTTTTTAAAATGTGTTTGTTAGGGATAGATTTCCACCCCTTTATAAATAATGGTCCCTTCCTCTCTCAAACCGTAGGGCAAAATCCCTGGATTTGGTGGATCTTACAGTCCACCCCCTTTGTAGCCCAGCGTCCTCGCTGTCACTTGTTCTCCTTTCTAATCGACGTGGGATCTCACATGTTCTCTATATCATGGAATTCAATTCTAGTTTCTTTTCTGTTAAAAACGGGCAATGACTTGGAGATCTGTTTATTGGTAAACTTTTAGTTTGAAACAAAACGCCAGCTAAGAAACAAGTAGATATTGCCCTCTTTGAGTTTCCACACTCTTATAAAGAATGTTTCGTTCTGCTCCCCAACCGATCTGGAATCTCACAAAAAAAAGTCGTTTGCTTAACTTCTCGTCATCTTCATCTTTCTTTTGCTCGTCGCATCACATTTTGTCTTCCAAAGAGAGTGTTTATTTGTTTATGGCTGCAGTTAAGCCAAAGAAGGGCGACGCTCTACTATTCTTCAGTCTCCATCCAAACGTGACGACAGACCCGACGAGCTATCACGGGAGCTGCCCAGTGATAGAGGGGGAGAAGTGGTCTGCAACAAAATGGATTCACATGCTACCAGTAGATGAGATTTGGAGGAATCCAGATTGTGTGGATGAGAATGAGCACTGTAGTGCATGGGCCAAAGCAGGTGAATGTGAAAAGAACCCTGGTTATATGGTGGGTTCTTCCTTGGGTTCTAAGGAAGATCTTGGATATTGTAGGCTTAGTTGCAAAGCCTGCTCTCCTCCCTCGTAA

mRNA sequence

ATGGTTAGCTCGAGTTTCGCCATGGATTCTCGATTTTTTCTTGCATTTTCTCTTTGTTTCCTCTGTTCATTCCCTCTATTTGCTCGCTCCGCCAATCGATTGCCGAAATTACTCTTGGACGACACGAAAACGGAAGATTCTGTTATTAGGATGAAAATGGACGGTTCCTCCATTAAAATCGATCCCACTCGCGTCGTTCAGCTTTCATCGCAACCTAGGGCTTTCTTATACAAGGGATTTTTGTCTGCAGAGGAGTGCCAGCATCTTATCGATTTGGATGACATAGTTGCTGGCATTGAGGCCAAGATTGCTGCGTGGACAATGAGGCTCGTAAAATCTCGAAATTGCTCTTTCGGATCAGATAATGGGGAGCCTATACAAATACTAAGGTATGAAAATGGTCAGCAATATGTACCACATTTTGATTTTTTTCAAGATCCAGTCAATGTAGCTGCTGGTGGTCATCGGATAGCCACAGTCTTGATGTATTTGTCCAATGTTGAAAGGGGTGGAGAAACTGTCTTTCCCGATTCTCCGCCAAAGAAGGGCGACGCTCTACTATTCTTCAGTCTCCATCCAAACGTGACGACAGACCCGACGAGCTATCACGGGAGCTGCCCAGTGATAGAGGGGGAGAAGTGGTCTGCAACAAAATGGATTCACATGCTACCAGTAGATGAGATTTGGAGGAATCCAGATTGTGTGGATGAGAATGAGCACTGTAGTGCATGGGCCAAAGCAGGTGAATGTGAAAAGAACCCTGGTTATATGGTGGGTTCTTCCTTGGGTTCTAAGGAAGATCTTGGATATTGTAGGCTTAGTTGCAAAGCCTGCTCTCCTCCCTCGTAA

Coding sequence (CDS)

ATGGTTAGCTCGAGTTTCGCCATGGATTCTCGATTTTTTCTTGCATTTTCTCTTTGTTTCCTCTGTTCATTCCCTCTATTTGCTCGCTCCGCCAATCGATTGCCGAAATTACTCTTGGACGACACGAAAACGGAAGATTCTGTTATTAGGATGAAAATGGACGGTTCCTCCATTAAAATCGATCCCACTCGCGTCGTTCAGCTTTCATCGCAACCTAGGGCTTTCTTATACAAGGGATTTTTGTCTGCAGAGGAGTGCCAGCATCTTATCGATTTGGATGACATAGTTGCTGGCATTGAGGCCAAGATTGCTGCGTGGACAATGAGGCTCGTAAAATCTCGAAATTGCTCTTTCGGATCAGATAATGGGGAGCCTATACAAATACTAAGGTATGAAAATGGTCAGCAATATGTACCACATTTTGATTTTTTTCAAGATCCAGTCAATGTAGCTGCTGGTGGTCATCGGATAGCCACAGTCTTGATGTATTTGTCCAATGTTGAAAGGGGTGGAGAAACTGTCTTTCCCGATTCTCCGCCAAAGAAGGGCGACGCTCTACTATTCTTCAGTCTCCATCCAAACGTGACGACAGACCCGACGAGCTATCACGGGAGCTGCCCAGTGATAGAGGGGGAGAAGTGGTCTGCAACAAAATGGATTCACATGCTACCAGTAGATGAGATTTGGAGGAATCCAGATTGTGTGGATGAGAATGAGCACTGTAGTGCATGGGCCAAAGCAGGTGAATGTGAAAAGAACCCTGGTTATATGGTGGGTTCTTCCTTGGGTTCTAAGGAAGATCTTGGATATTGTAGGCTTAGTTGCAAAGCCTGCTCTCCTCCCTCGTAA

Protein sequence

MVSSSFAMDSRFFLAFSLCFLCSFPLFARSANRLPKLLLDDTKTEDSVIRMKMDGSSIKIDPTRVVQLSSQPRAFLYKGFLSAEECQHLIDLDDIVAGIEAKIAAWTMRLVKSRNCSFGSDNGEPIQILRYENGQQYVPHFDFFQDPVNVAAGGHRIATVLMYLSNVERGGETVFPDSPPKKGDALLFFSLHPNVTTDPTSYHGSCPVIEGEKWSATKWIHMLPVDEIWRNPDCVDENEHCSAWAKAGECEKNPGYMVGSSLGSKEDLGYCRLSCKACSPPS
Homology
[Close]
BLAST of Cp4.1LG04g01360 vs. ExPASy Swiss-Prot
Match: Q8L970 (Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 4.5e-83
Identity = 168/330 (50.91%), Postives = 194/330 (58.79%), Query Frame = 0 

Query: 8   MDSRFFLAFSLCFLCSFPLFARSANRLPKLLLDDTKTEDSVIRMKMDGSSIKIDPTRVVQ 67
           MDSR FLAFSLCFL + PL + + NR   L       + SVI+MK   SS   DPTRV Q
Sbjct: 1   MDSRIFLAFSLCFLFTLPLISSAPNRF--LTRSSNTRDGSVIKMKTSASSFGFDPTRVTQ 60

Query: 68  LSSQPRAFLYKGFLSAEECQHLIDL----------------------------------- 127
           LS  PR FLY+GFLS EEC H I L                                   
Sbjct: 61  LSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEVRTSSGMFLSKRQ 120

Query: 128 DDIVAGIEAKIAAWTMRLVKSRNCSFGSDNGEPIQILRYENGQQYVPHFDFFQDPVNVAA 187
           DDIV+ +EAK+AAWT             +NGE +QIL YENGQ+Y PHFD+F D  N+  
Sbjct: 121 DDIVSNVEAKLAAWTF---------LPEENGESMQILHYENGQKYEPHFDYFHDQANLEL 180

Query: 188 GGHRIATVLMYLSNVERGGETVFP-----------DS-----------PPKKGDALLFFS 247
           GGHRIATVLMYLSNVE+GGETVFP           DS            P+KGDALLFF+
Sbjct: 181 GGHRIATVLMYLSNVEKGGETVFPMWKGKATQLKDDSWTECAKQGYAVKPRKGDALLFFN 240

Query: 248 LHPNVTTDPTSYHGSCPVIEGEKWSATKWIHMLPVDEIW-RNPDCVDENEHCSAWAKAGE 280
           LHPN TTD  S HGSCPV+EGEKWSAT+WIH+   +  + +   C+DEN  C  WAKAGE
Sbjct: 241 LHPNATTDSNSLHGSCPVVEGEKWSATRWIHVKSFERAFNKQSGCMDENVSCEKWAKAGE 300

[Close]
BLAST of Cp4.1LG04g01360 vs. ExPASy Swiss-Prot
Match: F4J0A8 (Probable prolyl 4-hydroxylase 6 OS=Arabidopsis thaliana OX=3702 GN=P4H6 PE=2 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 8.8e-71
Identity = 153/329 (46.50%), Postives = 178/329 (54.10%), Query Frame = 0 

Query: 8   MDSRFFLAFSLCFLCSFPLFARSANRLPKLLLDDTKTEDSVIRMKMDGSSIKIDPTRVVQ 67
           MDS++FLAFSL  L  F                           ++   S  +DPTR+ Q
Sbjct: 1   MDSQYFLAFSLSLLLIF--------------------------SQISSFSFSVDPTRITQ 60

Query: 68  LSSQPRAFLYKGFLSAEECQHLIDL----------------------------------- 127
           LS  PRAFLYKGFLS EEC HLI L                                   
Sbjct: 61  LSWTPRAFLYKGFLSDEECDHLIKLAKGKLEKSMVVADVDSGESEDSEVRTSSGMFLTKR 120

Query: 128 -DDIVAGIEAKIAAWTMRLVKSRNCSFGSDNGEPIQILRYENGQQYVPHFDFFQDPVNVA 187
            DDIVA +EAK+AAWT             +NGE +QIL YENGQ+Y PHFD+F D   + 
Sbjct: 121 QDDIVANVEAKLAAWTF---------LPEENGEALQILHYENGQKYDPHFDYFYDKKALE 180

Query: 188 AGGHRIATVLMYLSNVERGGETVFPD----------------------SPPKKGDALLFF 247
            GGHRIATVLMYLSNV +GGETVFP+                        P+KGDALLFF
Sbjct: 181 LGGHRIATVLMYLSNVTKGGETVFPNWKGKTPQLKDDSWSKCAKQGYAVKPRKGDALLFF 240

Query: 248 SLHPNVTTDPTSYHGSCPVIEGEKWSATKWIHMLPVDEIWRNPDCVDENEHCSAWAKAGE 279
           +LH N TTDP S HGSCPVIEGEKWSAT+WIH+    +  +   CVD++E C  WA AGE
Sbjct: 241 NLHLNGTTDPNSLHGSCPVIEGEKWSATRWIHVRSFGK--KKLVCVDDHESCQEWADAGE 288

[Close]
BLAST of Cp4.1LG04g01360 vs. ExPASy Swiss-Prot
Match: Q8LAN3 (Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 1.1e-65
Identity = 131/287 (45.64%), Postives = 164/287 (57.14%), Query Frame = 0 

Query: 53  MDGSSIKIDPTRVVQLSSQPRAFLYKGFLSAEECQHLIDL-------------------- 112
           +  SS+ ++P++V Q+SS+PRAF+Y+GFL+  EC H++ L                    
Sbjct: 25  ISSSSVFVNPSKVKQVSSKPRAFVYEGFLTELECDHMVSLAKASLKRSAVADNDSGESKF 84

Query: 113 ---------------DDIVAGIEAKIAAWTMRLVKSRNCSFGSDNGEPIQILRYENGQQY 172
                          D IV+GIE KI+ WT             +NGE IQ+LRYE+GQ+Y
Sbjct: 85  SEVRTSSGTFISKGKDPIVSGIEDKISTWTF---------LPKENGEDIQVLRYEHGQKY 144

Query: 173 VPHFDFFQDPVNVAAGGHRIATVLMYLSNVERGGETVFPDS------------------- 232
             HFD+F D VN+  GGHR+AT+LMYLSNV +GGETVFPD+                   
Sbjct: 145 DAHFDYFHDKVNIVRGGHRMATILMYLSNVTKGGETVFPDAEIPSRRVLSENKEDLSDCA 204

Query: 233 ------PPKKGDALLFFSLHPNVTTDPTSYHGSCPVIEGEKWSATKWIHMLPVDEI-WRN 279
                  P+KGDALLFF+LHP+   DP S HG CPVIEGEKWSATKWIH+   D I   +
Sbjct: 205 KRGIAVKPRKGDALLFFNLHPDAIPDPLSLHGGCPVIEGEKWSATKWIHVDSFDRIVTPS 264

[Close]
BLAST of Cp4.1LG04g01360 vs. ExPASy Swiss-Prot
Match: F4JAU3 (Prolyl 4-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=P4H2 PE=1 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 4.4e-62
Identity = 129/280 (46.07%), Postives = 158/280 (56.43%), Query Frame = 0 

Query: 60  IDPTRVVQLSSQPRAFLYKGFLSAEECQHLIDL--------------------------- 119
           I+P++V Q+SS+PRAF+Y+GFL+  EC HLI L                           
Sbjct: 33  INPSKVKQVSSKPRAFVYEGFLTDLECDHLISLAKENLQRSAVADNDNGESQVSDVRTSS 92

Query: 120 --------DDIVAGIEAKIAAWTMRLVKSRNCSFGSDNGEPIQILRYENGQQYVPHFDFF 179
                   D IV+GIE K++ WT             +NGE +Q+LRYE+GQ+Y  HFD+F
Sbjct: 93  GTFISKGKDPIVSGIEDKLSTWTF---------LPKENGEDLQVLRYEHGQKYDAHFDYF 152

Query: 180 QDPVNVAAGGHRIATVLMYLSNVERGGETVFPDS-------------------------P 239
            D VN+A GGHRIATVL+YLSNV +GGETVFPD+                          
Sbjct: 153 HDKVNIARGGHRIATVLLYLSNVTKGGETVFPDAQEFSRRSLSENKDDLSDCAKKGIAVK 212

Query: 240 PKKGDALLFFSLHPNVTTDPTSYHGSCPVIEGEKWSATKWIHMLPVDEI-WRNPDCVDEN 279
           PKKG+ALLFF+L  +   DP S HG CPVIEGEKWSATKWIH+   D+I   + +C D N
Sbjct: 213 PKKGNALLFFNLQQDAIPDPFSLHGGCPVIEGEKWSATKWIHVDSFDKILTHDGNCTDVN 272

[Close]
BLAST of Cp4.1LG04g01360 vs. ExPASy Swiss-Prot
Match: Q8GXT7 (Probable prolyl 4-hydroxylase 12 OS=Arabidopsis thaliana OX=3702 GN=P4H12 PE=2 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 4.5e-43
Identity = 111/302 (36.75%), Postives = 149/302 (49.34%), Query Frame = 0 

Query: 10  SRFFLAFSLCFLCSFPLFARSANRLPKLLLDDTKTEDSVIRMKMDGSSIKIDPTRVVQLS 69
           SR FL   +    S P F    +R      + T   D      + GS   +DPTRV+QLS
Sbjct: 5   SRIFLILMITMSSSSPPFCSGGSRKELRDKEITSKSDDTQASYVLGSKF-VDPTRVLQLS 64

Query: 70  SQPRAFLYKGFLSAEECQHLI------------------DLDDIVAGIEAKIAAWTMRLV 129
             PR FLY+GFLS EEC HLI                   LD +VAGIE K++AWT    
Sbjct: 65  WLPRVFLYRGFLSEEECDHLISLRKETTEVYSVDADGKTQLDPVVAGIEEKVSAWTF--- 124

Query: 130 KSRNCSFGSDNGEPIQILRYENGQQYVPHFDFFQDPVNVAAGGHRIATVLMYLSNVERGG 189
                    +NG  I++  Y   ++     D+F +  +       +ATV++YLSN  +GG
Sbjct: 125 ------LPGENGGSIKVRSY-TSEKSGKKLDYFGEEPSSVLHESLLATVVLYLSNTTQGG 184

Query: 190 ETVFPDSP---------------PKKGDALLFFSLHPNVTTDPTSYHGSCPVIEGEKWSA 249
           E +FP+S                P KG+A+LFF+   N + D  S H  CPV++GE   A
Sbjct: 185 ELLFPNSEMKPKNSCLEGGNILRPVKGNAILFFTRLLNASLDGKSTHLRCPVVKGELLVA 244

Query: 250 TKWIHMLPVDEIWRNPDCVDENEHCSAWAKAGECEKNPGYMVGSSLGSKEDLGYCRLSCK 279
           TK I+      I  + +C DE+E+C  WAK GEC+KNP YM+    GS +  G CR SC 
Sbjct: 245 TKLIYAKKQARIEESGECSDEDENCGRWAKLGECKKNPVYMI----GSPDYYGTCRKSCN 291

[Close]
BLAST of Cp4.1LG04g01360 vs. NCBI nr
Match: XP_023530715.1 (probable prolyl 4-hydroxylase 7 [Cucurbita pepo subsp. pepo] >XP_023530716.1 probable prolyl 4-hydroxylase 7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 514 bits (1324), Expect = 2.36e-182
Identity = 263/332 (79.22%), Postives = 263/332 (79.22%), Query Frame = 0 

Query: 8   MDSRFFLAFSLCFLCSFPLFARSANRLPKLLLDDTKTEDSVIRMKMDGSSIKIDPTRVVQ 67
           MDSRFFLAFSLCFLCSFPLFARSANRLPKLLLDDTKTEDSVIRMKMDGSSIKIDPTRVVQ
Sbjct: 1   MDSRFFLAFSLCFLCSFPLFARSANRLPKLLLDDTKTEDSVIRMKMDGSSIKIDPTRVVQ 60

Query: 68  LSSQPRAFLYKGFLSAEECQHLIDL----------------------------------- 127
           LSSQPRAFLYKGFLSAEECQHLIDL                                   
Sbjct: 61  LSSQPRAFLYKGFLSAEECQHLIDLAKDYLEQSLVVDDITGASRSSTDRTSTGMFLYKAQ 120

Query: 128 DDIVAGIEAKIAAWTMRLVKSRNCSFGSDNGEPIQILRYENGQQYVPHFDFFQDPVNVAA 187
           DDIVAGIEAKIAAWT   V         DNGEPIQILRYENGQQYVPHFDFFQDPVNVAA
Sbjct: 121 DDIVAGIEAKIAAWTFLPV---------DNGEPIQILRYENGQQYVPHFDFFQDPVNVAA 180

Query: 188 GGHRIATVLMYLSNVERGGETVFPDSP----------------------PKKGDALLFFS 247
           GGHRIATVLMYLSNVERGGETVFPDSP                      PKKGDALLFFS
Sbjct: 181 GGHRIATVLMYLSNVERGGETVFPDSPAKVFEEENKDLSDCSTTGYGVKPKKGDALLFFS 240

Query: 248 LHPNVTTDPTSYHGSCPVIEGEKWSATKWIHMLPVDEIWRNPDCVDENEHCSAWAKAGEC 282
           LHPNVTTDPTSYHGSCPVIEGEKWSATKWIHMLPVDEIWRNPDCVDENEHCSAWAKAGEC
Sbjct: 241 LHPNVTTDPTSYHGSCPVIEGEKWSATKWIHMLPVDEIWRNPDCVDENEHCSAWAKAGEC 300

[Close]
BLAST of Cp4.1LG04g01360 vs. NCBI nr
Match: XP_022931100.1 (probable prolyl 4-hydroxylase 7 [Cucurbita moschata])

HSP 1 Score: 513 bits (1320), Expect = 9.61e-182
Identity = 262/332 (78.92%), Postives = 263/332 (79.22%), Query Frame = 0 

Query: 8   MDSRFFLAFSLCFLCSFPLFARSANRLPKLLLDDTKTEDSVIRMKMDGSSIKIDPTRVVQ 67
           MDSRFFLAFSLCFLCSFPLFARSANRLPKLLLDDTKTEDSVIRMKMDGSSIKIDPTRVVQ
Sbjct: 1   MDSRFFLAFSLCFLCSFPLFARSANRLPKLLLDDTKTEDSVIRMKMDGSSIKIDPTRVVQ 60

Query: 68  LSSQPRAFLYKGFLSAEECQHLIDL----------------------------------- 127
           LSSQPRAFLYKGFLSAEECQHLIDL                                   
Sbjct: 61  LSSQPRAFLYKGFLSAEECQHLIDLAKDYLEQSLVVDDITGASRSSTDRTSTGMFLYKAQ 120

Query: 128 DDIVAGIEAKIAAWTMRLVKSRNCSFGSDNGEPIQILRYENGQQYVPHFDFFQDPVNVAA 187
           DDIVAGIEAKIAAWT   V         DNGEPIQILRYENGQQYVPHFDFFQDPVNVAA
Sbjct: 121 DDIVAGIEAKIAAWTFLPV---------DNGEPIQILRYENGQQYVPHFDFFQDPVNVAA 180

Query: 188 GGHRIATVLMYLSNVERGGETVFPDSP----------------------PKKGDALLFFS 247
           GGHRIATVLMYLSNVERGGETVFPDSP                      PKKGDALLFFS
Sbjct: 181 GGHRIATVLMYLSNVERGGETVFPDSPAKVFEEENKDLFDCSTTGYGVKPKKGDALLFFS 240

Query: 248 LHPNVTTDPTSYHGSCPVIEGEKWSATKWIHMLPVDEIWRNPDCVDENEHCSAWAKAGEC 282
           LHPNVTTDPTSYHGSCPVIEGEKWSATKWIHMLPVDEIWRNPDCVDENEHCSAWAKAGEC
Sbjct: 241 LHPNVTTDPTSYHGSCPVIEGEKWSATKWIHMLPVDEIWRNPDCVDENEHCSAWAKAGEC 300

[Close]
BLAST of Cp4.1LG04g01360 vs. NCBI nr
Match: XP_022971148.1 (probable prolyl 4-hydroxylase 7 [Cucurbita maxima] >XP_022971154.1 probable prolyl 4-hydroxylase 7 [Cucurbita maxima])

HSP 1 Score: 510 bits (1313), Expect = 1.08e-180
Identity = 259/331 (78.25%), Postives = 263/331 (79.46%), Query Frame = 0 

Query: 8   MDSRFFLAFSLCFLCSFPLFARSANRLPKLLLDDTKTEDSVIRMKMDGSSIKIDPTRVVQ 67
           MDSRFFLAFSLCFLCSFPLFARSANRLPKLLLDDTKTEDSVIRMKMDGSSIKIDPTRVVQ
Sbjct: 1   MDSRFFLAFSLCFLCSFPLFARSANRLPKLLLDDTKTEDSVIRMKMDGSSIKIDPTRVVQ 60

Query: 68  LSSQPRAFLYKGFLSAEECQHLIDL----------------------------------- 127
           LSSQPRAFLYKGFLSAEECQHLIDL                                   
Sbjct: 61  LSSQPRAFLYKGFLSAEECQHLIDLAKDYLEQSLVVDDITGASRSSTDRTSTGMFLYKAQ 120

Query: 128 DDIVAGIEAKIAAWTMRLVKSRNCSFGSDNGEPIQILRYENGQQYVPHFDFFQDPVNVAA 187
           DDIVAGIEAKIAAWT   V         DNGEP+QILRYENGQQYVPHFDFFQDPVNVAA
Sbjct: 121 DDIVAGIEAKIAAWTFLPV---------DNGEPLQILRYENGQQYVPHFDFFQDPVNVAA 180

Query: 188 GGHRIATVLMYLSNVERGGETVFPDSP---------------------PKKGDALLFFSL 247
           GGHRIATVL+YLSNVERGGETVFPDSP                     PKKGDALLFFSL
Sbjct: 181 GGHRIATVLLYLSNVERGGETVFPDSPAKVFEENKDLSDCSTTGYGVKPKKGDALLFFSL 240

Query: 248 HPNVTTDPTSYHGSCPVIEGEKWSATKWIHMLPVDEIWRNPDCVDENEHCSAWAKAGECE 282
           HPNVTTDPTSYHGSCPVIEGEKWSATKWIHMLP+DEIWRNPDCVDENEHCSAWAKAGECE
Sbjct: 241 HPNVTTDPTSYHGSCPVIEGEKWSATKWIHMLPLDEIWRNPDCVDENEHCSAWAKAGECE 300

[Close]
BLAST of Cp4.1LG04g01360 vs. NCBI nr
Match: KAG6588394.1 (putative prolyl 4-hydroxylase 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 499 bits (1285), Expect = 1.78e-176
Identity = 255/329 (77.51%), Postives = 259/329 (78.72%), Query Frame = 0 

Query: 8   MDSRFFLAFSLCFLCSFPLFARSANRLPKLLLDDTKTEDSVIRMKMDGSSIKIDPTRVVQ 67
           MDSRFFLAFSLCFLCSFPLFAR ANRLPKLLLDDTKTEDSVIRMKMDGSSIKIDPTRVVQ
Sbjct: 1   MDSRFFLAFSLCFLCSFPLFARPANRLPKLLLDDTKTEDSVIRMKMDGSSIKIDPTRVVQ 60

Query: 68  LSSQPRAFLYKGFLSAEECQHLIDL--------------------------------DDI 127
           LSSQPRAFLYKGFLSAEEC H++ +                                DDI
Sbjct: 61  LSSQPRAFLYKGFLSAEEC-HILSIWYEQSLVVDDITGASRSSTDRTSTGMFLYKAQDDI 120

Query: 128 VAGIEAKIAAWTMRLVKSRNCSFGSDNGEPIQILRYENGQQYVPHFDFFQDPVNVAAGGH 187
           VAGIEAKIAAWT   V         DNGEPIQILRYENGQQYVPHFDFFQDPVNVAAGGH
Sbjct: 121 VAGIEAKIAAWTFLPV---------DNGEPIQILRYENGQQYVPHFDFFQDPVNVAAGGH 180

Query: 188 RIATVLMYLSNVERGGETVFPDSP----------------------PKKGDALLFFSLHP 247
           RIATVLMYLSNVERGGETVFPDSP                      PKKGDALLFFSLHP
Sbjct: 181 RIATVLMYLSNVERGGETVFPDSPAKVFEEENKDLFDCSTTGYGVKPKKGDALLFFSLHP 240

Query: 248 NVTTDPTSYHGSCPVIEGEKWSATKWIHMLPVDEIWRNPDCVDENEHCSAWAKAGECEKN 282
           NVTTDPTSYHGSCPVIEGEKWSATKWIHMLPVDEIWRNPDCVDENEHCS WAKAGECEKN
Sbjct: 241 NVTTDPTSYHGSCPVIEGEKWSATKWIHMLPVDEIWRNPDCVDENEHCSVWAKAGECEKN 300

[Close]
BLAST of Cp4.1LG04g01360 vs. NCBI nr
Match: KAG7022241.1 (putative prolyl 4-hydroxylase 7 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 481 bits (1238), Expect = 3.01e-170
Identity = 241/287 (83.97%), Postives = 243/287 (84.67%), Query Frame = 0 

Query: 8   MDSRFFLAFSLCFLCSFPLFARSANRLPKLLLDDTKTEDSVIRMKMDGSSIKIDPTRVVQ 67
           MDSRFFLAFSLCFLCSFPLFAR ANRLPKLLLDDTKTEDSVIRMKMDGSSIKIDPTRVVQ
Sbjct: 1   MDSRFFLAFSLCFLCSFPLFARPANRLPKLLLDDTKTEDSVIRMKMDGSSIKIDPTRVVQ 60

Query: 68  LSSQPRAFLYKGFLSAEECQHLIDLDDIVAGIEAKIAAWTMRLVKSRNCSFGSDNGEPIQ 127
           LSSQPR+                  DDIVAGIEAKIAAWT   V         DNGEPIQ
Sbjct: 61  LSSQPRS------------------DDIVAGIEAKIAAWTFLPV---------DNGEPIQ 120

Query: 128 ILRYENGQQYVPHFDFFQDPVNVAAGGHRIATVLMYLSNVERGGETVFPDSP-------- 187
           ILRYENGQQYVPHFDFFQDPVNVAAGGHRIATVLMYLSNVERGGETVFPDSP        
Sbjct: 121 ILRYENGQQYVPHFDFFQDPVNVAAGGHRIATVLMYLSNVERGGETVFPDSPVLLSPNAN 180

Query: 188 ----PKKGDALLFFSLHPNVTTDPTSYHGSCPVIEGEKWSATKWIHMLPVDEIWRNPDCV 247
               PKKGDALLFFSLHPNVTTDPTSYHGSCPVIEGEKWSATKWIHMLPVDEIWRNPDCV
Sbjct: 181 CSLKPKKGDALLFFSLHPNVTTDPTSYHGSCPVIEGEKWSATKWIHMLPVDEIWRNPDCV 240

Query: 248 DENEHCSAWAKAGECEKNPGYMVGSSLGSKEDLGYCRLSCKACSPPS 282
           DENEHCS WAKAGECEKNPGYMVGSSLGSKE+LGYCRLSCKACSPPS
Sbjct: 241 DENEHCSVWAKAGECEKNPGYMVGSSLGSKEELGYCRLSCKACSPPS 260

[Close]
BLAST of Cp4.1LG04g01360 vs. ExPASy TrEMBL
Match: A0A6J1EYJ1 (Procollagen-proline 4-dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111437385 PE=3 SV=1)

HSP 1 Score: 513 bits (1320), Expect = 4.65e-182
Identity = 262/332 (78.92%), Postives = 263/332 (79.22%), Query Frame = 0 

Query: 8   MDSRFFLAFSLCFLCSFPLFARSANRLPKLLLDDTKTEDSVIRMKMDGSSIKIDPTRVVQ 67
           MDSRFFLAFSLCFLCSFPLFARSANRLPKLLLDDTKTEDSVIRMKMDGSSIKIDPTRVVQ
Sbjct: 1   MDSRFFLAFSLCFLCSFPLFARSANRLPKLLLDDTKTEDSVIRMKMDGSSIKIDPTRVVQ 60

Query: 68  LSSQPRAFLYKGFLSAEECQHLIDL----------------------------------- 127
           LSSQPRAFLYKGFLSAEECQHLIDL                                   
Sbjct: 61  LSSQPRAFLYKGFLSAEECQHLIDLAKDYLEQSLVVDDITGASRSSTDRTSTGMFLYKAQ 120

Query: 128 DDIVAGIEAKIAAWTMRLVKSRNCSFGSDNGEPIQILRYENGQQYVPHFDFFQDPVNVAA 187
           DDIVAGIEAKIAAWT   V         DNGEPIQILRYENGQQYVPHFDFFQDPVNVAA
Sbjct: 121 DDIVAGIEAKIAAWTFLPV---------DNGEPIQILRYENGQQYVPHFDFFQDPVNVAA 180

Query: 188 GGHRIATVLMYLSNVERGGETVFPDSP----------------------PKKGDALLFFS 247
           GGHRIATVLMYLSNVERGGETVFPDSP                      PKKGDALLFFS
Sbjct: 181 GGHRIATVLMYLSNVERGGETVFPDSPAKVFEEENKDLFDCSTTGYGVKPKKGDALLFFS 240

Query: 248 LHPNVTTDPTSYHGSCPVIEGEKWSATKWIHMLPVDEIWRNPDCVDENEHCSAWAKAGEC 282
           LHPNVTTDPTSYHGSCPVIEGEKWSATKWIHMLPVDEIWRNPDCVDENEHCSAWAKAGEC
Sbjct: 241 LHPNVTTDPTSYHGSCPVIEGEKWSATKWIHMLPVDEIWRNPDCVDENEHCSAWAKAGEC 300

[Close]
BLAST of Cp4.1LG04g01360 vs. ExPASy TrEMBL
Match: A0A6J1I5Z9 (Procollagen-proline 4-dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111469906 PE=3 SV=1)

HSP 1 Score: 510 bits (1313), Expect = 5.23e-181
Identity = 259/331 (78.25%), Postives = 263/331 (79.46%), Query Frame = 0 

Query: 8   MDSRFFLAFSLCFLCSFPLFARSANRLPKLLLDDTKTEDSVIRMKMDGSSIKIDPTRVVQ 67
           MDSRFFLAFSLCFLCSFPLFARSANRLPKLLLDDTKTEDSVIRMKMDGSSIKIDPTRVVQ
Sbjct: 1   MDSRFFLAFSLCFLCSFPLFARSANRLPKLLLDDTKTEDSVIRMKMDGSSIKIDPTRVVQ 60

Query: 68  LSSQPRAFLYKGFLSAEECQHLIDL----------------------------------- 127
           LSSQPRAFLYKGFLSAEECQHLIDL                                   
Sbjct: 61  LSSQPRAFLYKGFLSAEECQHLIDLAKDYLEQSLVVDDITGASRSSTDRTSTGMFLYKAQ 120

Query: 128 DDIVAGIEAKIAAWTMRLVKSRNCSFGSDNGEPIQILRYENGQQYVPHFDFFQDPVNVAA 187
           DDIVAGIEAKIAAWT   V         DNGEP+QILRYENGQQYVPHFDFFQDPVNVAA
Sbjct: 121 DDIVAGIEAKIAAWTFLPV---------DNGEPLQILRYENGQQYVPHFDFFQDPVNVAA 180

Query: 188 GGHRIATVLMYLSNVERGGETVFPDSP---------------------PKKGDALLFFSL 247
           GGHRIATVL+YLSNVERGGETVFPDSP                     PKKGDALLFFSL
Sbjct: 181 GGHRIATVLLYLSNVERGGETVFPDSPAKVFEENKDLSDCSTTGYGVKPKKGDALLFFSL 240

Query: 248 HPNVTTDPTSYHGSCPVIEGEKWSATKWIHMLPVDEIWRNPDCVDENEHCSAWAKAGECE 282
           HPNVTTDPTSYHGSCPVIEGEKWSATKWIHMLP+DEIWRNPDCVDENEHCSAWAKAGECE
Sbjct: 241 HPNVTTDPTSYHGSCPVIEGEKWSATKWIHMLPLDEIWRNPDCVDENEHCSAWAKAGECE 300

[Close]
BLAST of Cp4.1LG04g01360 vs. ExPASy TrEMBL
Match: A0A1S3B814 (Procollagen-proline 4-dioxygenase OS=Cucumis melo OX=3656 GN=LOC103487037 PE=3 SV=1)

HSP 1 Score: 384 bits (986), Expect = 2.65e-131
Identity = 200/331 (60.42%), Postives = 226/331 (68.28%), Query Frame = 0 

Query: 8   MDSRFFLAFSLCFLCSFPLFARSANRLPKLLLDDTKTEDSVIRMKMDGSSIKIDPTRVVQ 67
           M S F LAFS+ FL   PL + SANR PK+LL +    +SVIRMK  GS+I IDPTRV+Q
Sbjct: 1   MASPFLLAFSIFFLWLLPLSSLSANRFPKMLLHNNDMYESVIRMKTGGSAITIDPTRVIQ 60

Query: 68  LSSQPRAFLYKGFLSAEECQHLIDL----------------------------------D 127
           LSS+PRAFLYKGFLS EECQHLI L                                  D
Sbjct: 61  LSSKPRAFLYKGFLSYEECQHLIHLAKGKLRQSLVAAGTGESVTSKERTSTGMFLRKAQD 120

Query: 128 DIVAGIEAKIAAWTMRLVKSRNCSFGSDNGEPIQILRYENGQQYVPHFDFFQDPVNVAAG 187
            IVA IE++IAAWT   +         DNGEPIQILRYENGQ+Y PHFDFFQDP N+A G
Sbjct: 121 KIVARIESRIAAWTFLPL---------DNGEPIQILRYENGQKYEPHFDFFQDPGNIAIG 180

Query: 188 GHRIATVLMYLSNVERGGETVFPDSP----------------------PKKGDALLFFSL 247
           GHRIAT+LMYLS+VE+GGETVFP+SP                      PK GDALLFFS+
Sbjct: 181 GHRIATILMYLSDVEKGGETVFPNSPVKLSEEEKGDLSECAKVGYGVRPKLGDALLFFSM 240

Query: 248 HPNVTTDPTSYHGSCPVIEGEKWSATKWIHMLPVDEIWRNPDCVDENEHCSAWAKAGECE 282
           +PNVT D TSYHGSCPVIEGEKWSATKWIHMLP+DE+WRNP CVDEN+HCSAWAKAGEC+
Sbjct: 241 NPNVTPDATSYHGSCPVIEGEKWSATKWIHMLPIDEVWRNPACVDENDHCSAWAKAGECK 300

[Close]
BLAST of Cp4.1LG04g01360 vs. ExPASy TrEMBL
Match: A0A0A0LG32 (Procollagen-proline 4-dioxygenase OS=Cucumis sativus OX=3659 GN=Csa_3G828960 PE=3 SV=1)

HSP 1 Score: 381 bits (978), Expect = 4.52e-130
Identity = 198/331 (59.82%), Postives = 226/331 (68.28%), Query Frame = 0 

Query: 8   MDSRFFLAFSLCFLCSF--PLFARSANRLPKLLLDDTKTEDSVIRMKMDGSSIKIDPTRV 67
           M S FFL FS+ FL  F  P  + SANR PKL+L +   ++SVIRMK  GS++ IDPTRV
Sbjct: 1   MASPFFLPFSIFFLFLFLLPFSSLSANRFPKLILHNNDIDESVIRMKTGGSAMTIDPTRV 60

Query: 68  VQLSSQPRAFLYKGFLSAEECQHLIDL--------------------------------- 127
           +QLSS+PRAFLYKGFLSAEECQHLI+                                  
Sbjct: 61  IQLSSKPRAFLYKGFLSAEECQHLINSAKGKLHQSLVAAGTGQSVTSKERTSTGMFLHKA 120

Query: 128 -DDIVAGIEAKIAAWTMRLVKSRNCSFGSDNGEPIQILRYENGQQYVPHFDFFQDPVNVA 187
            D+IVA IE++IAAWT   +         DNGEPIQILRYENGQ+Y PHFDFFQDP N+A
Sbjct: 121 QDEIVARIESRIAAWTFLPL---------DNGEPIQILRYENGQKYEPHFDFFQDPGNIA 180

Query: 188 AGGHRIATVLMYLSNVERGGETVFPDSP----------------------PKKGDALLFF 247
            GGHRIAT+LMYLSNVE+GGETVFP+SP                      PK GDALLFF
Sbjct: 181 IGGHRIATILMYLSNVEKGGETVFPNSPVKLSEEEKADLSECGKVGYGVRPKLGDALLFF 240

Query: 248 SLHPNVTTDPTSYHGSCPVIEGEKWSATKWIHMLPVDEIWRNPDCVDENEHCSAWAKAGE 280
           S++PNVT D TSYHGSCPVIEGEKWSATKWIHMLP+DE WRNP CVDEN+HC+AWAKAGE
Sbjct: 241 SMNPNVTPDTTSYHGSCPVIEGEKWSATKWIHMLPIDEFWRNPACVDENDHCTAWAKAGE 300

[Close]
BLAST of Cp4.1LG04g01360 vs. ExPASy TrEMBL
Match: A0A6J1DTY4 (Procollagen-proline 4-dioxygenase OS=Momordica charantia OX=3673 GN=LOC111024321 PE=3 SV=1)

HSP 1 Score: 384 bits (986), Expect = 5.12e-130
Identity = 203/335 (60.60%), Postives = 226/335 (67.46%), Query Frame = 0 

Query: 3   SSSFAMDSRFFLAFSLCFLCSFPLFARSANRLPKLLLD-DTKTEDSVIRMKMDGSSIKID 62
           SSSF MDSR FLAFSLCFLC FPLF RS N +P+LL+D +     S+IRMK  GSSI ID
Sbjct: 81  SSSFHMDSRRFLAFSLCFLCLFPLFCRSTNPMPRLLMDRNNMGRGSLIRMKTGGSSISID 140

Query: 63  PTRVVQLSSQPRAFLYKGFLSAEECQHLIDL----------------------------- 122
           P+RV QLSSQPRAF+YKGFLSAEEC+HLI+L                             
Sbjct: 141 PSRVTQLSSQPRAFVYKGFLSAEECEHLINLAKDKLEESLVADDVTGESVTSRERTSTGM 200

Query: 123 ------DDIVAGIEAKIAAWTMRLVKSRNCSFGSDNGEPIQILRYENGQQYVPHFDFFQD 182
                 D IVAGIE++IAAWT   V         DNGEP+Q+LRYENGQ+Y PHFDFFQD
Sbjct: 201 FLVKGQDKIVAGIESRIAAWTFLPV---------DNGEPMQVLRYENGQKYDPHFDFFQD 260

Query: 183 PVNVAAGGHRIATVLMYLSNVERGGETVFPDS----------------------PPKKGD 242
           PVN+A GGHRIATVLMYLSNVE GGETVFP+S                       PK GD
Sbjct: 261 PVNMAQGGHRIATVLMYLSNVEEGGETVFPNSHVKLSAREKKELSDCAKLGYAVKPKMGD 320

Query: 243 ALLFFSLHPNVTTDPTSYHGSCPVIEGEKWSATKWIHMLPVDEIWRNPDCVDENEHCSAW 279
           ALLFFSLH N TTD +SYHGSCPVI+GEKWSATKWIHML  DEIWR+PDCVD +  C+AW
Sbjct: 321 ALLFFSLHANGTTDSSSYHGSCPVIKGEKWSATKWIHMLSADEIWRSPDCVDISVSCAAW 380

[Close]
BLAST of Cp4.1LG04g01360 vs. TAIR 10
Match: AT3G28480.1 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 309.3 bits (791), Expect = 3.2e-84
Identity = 168/330 (50.91%), Postives = 194/330 (58.79%), Query Frame = 0 

Query: 8   MDSRFFLAFSLCFLCSFPLFARSANRLPKLLLDDTKTEDSVIRMKMDGSSIKIDPTRVVQ 67
           MDSR FLAFSLCFL + PL + + NR   L       + SVI+MK   SS   DPTRV Q
Sbjct: 1   MDSRIFLAFSLCFLFTLPLISSAPNRF--LTRSSNTRDGSVIKMKTSASSFGFDPTRVTQ 60

Query: 68  LSSQPRAFLYKGFLSAEECQHLIDL----------------------------------- 127
           LS  PR FLY+GFLS EEC H I L                                   
Sbjct: 61  LSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEVRTSSGMFLSKRQ 120

Query: 128 DDIVAGIEAKIAAWTMRLVKSRNCSFGSDNGEPIQILRYENGQQYVPHFDFFQDPVNVAA 187
           DDIV+ +EAK+AAWT             +NGE +QIL YENGQ+Y PHFD+F D  N+  
Sbjct: 121 DDIVSNVEAKLAAWTF---------LPEENGESMQILHYENGQKYEPHFDYFHDQANLEL 180

Query: 188 GGHRIATVLMYLSNVERGGETVFP-----------DS-----------PPKKGDALLFFS 247
           GGHRIATVLMYLSNVE+GGETVFP           DS            P+KGDALLFF+
Sbjct: 181 GGHRIATVLMYLSNVEKGGETVFPMWKGKATQLKDDSWTECAKQGYAVKPRKGDALLFFN 240

Query: 248 LHPNVTTDPTSYHGSCPVIEGEKWSATKWIHMLPVDEIW-RNPDCVDENEHCSAWAKAGE 280
           LHPN TTD  S HGSCPV+EGEKWSAT+WIH+   +  + +   C+DEN  C  WAKAGE
Sbjct: 241 LHPNATTDSNSLHGSCPVVEGEKWSATRWIHVKSFERAFNKQSGCMDENVSCEKWAKAGE 300

[Close]
BLAST of Cp4.1LG04g01360 vs. TAIR 10
Match: AT3G28480.2 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 306.6 bits (784), Expect = 2.1e-83
Identity = 167/338 (49.41%), Postives = 195/338 (57.69%), Query Frame = 0 

Query: 8   MDSRFFLAFSLCFLCSFPLFARSANRLPKLLLDDTKTEDSVIRMKMDGSSIKIDPTRVVQ 67
           MDSR FLAFSLCFL + PL + + NR   L       + SVI+MK   SS   DPTRV Q
Sbjct: 1   MDSRIFLAFSLCFLFTLPLISSAPNRF--LTRSSNTRDGSVIKMKTSASSFGFDPTRVTQ 60

Query: 68  LSSQPRAFLYKGFLSAEECQHLI------------------------------------- 127
           LS  PR FLY+GFLS EEC H I                                     
Sbjct: 61  LSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEDSVSVVRQSSSFI 120

Query: 128 ------DLDDIVAGIEAKIAAWTMRLVKSRNCSFGSDNGEPIQILRYENGQQYVPHFDFF 187
                 ++DDIV+ +EAK+AAWT             +NGE +QIL YENGQ+Y PHFD+F
Sbjct: 121 ANMDSLEIDDIVSNVEAKLAAWTF---------LPEENGESMQILHYENGQKYEPHFDYF 180

Query: 188 QDPVNVAAGGHRIATVLMYLSNVERGGETVFP-----------DS-----------PPKK 247
            D  N+  GGHRIATVLMYLSNVE+GGETVFP           DS            P+K
Sbjct: 181 HDQANLELGGHRIATVLMYLSNVEKGGETVFPMWKGKATQLKDDSWTECAKQGYAVKPRK 240

Query: 248 GDALLFFSLHPNVTTDPTSYHGSCPVIEGEKWSATKWIHMLPVDEIW-RNPDCVDENEHC 280
           GDALLFF+LHPN TTD  S HGSCPV+EGEKWSAT+WIH+   +  + +   C+DEN  C
Sbjct: 241 GDALLFFNLHPNATTDSNSLHGSCPVVEGEKWSATRWIHVKSFERAFNKQSGCMDENVSC 300

[Close]
BLAST of Cp4.1LG04g01360 vs. TAIR 10
Match: AT3G28490.1 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 268.5 bits (685), Expect = 6.2e-72
Identity = 153/329 (46.50%), Postives = 178/329 (54.10%), Query Frame = 0 

Query: 8   MDSRFFLAFSLCFLCSFPLFARSANRLPKLLLDDTKTEDSVIRMKMDGSSIKIDPTRVVQ 67
           MDS++FLAFSL  L  F                           ++   S  +DPTR+ Q
Sbjct: 1   MDSQYFLAFSLSLLLIF--------------------------SQISSFSFSVDPTRITQ 60

Query: 68  LSSQPRAFLYKGFLSAEECQHLIDL----------------------------------- 127
           LS  PRAFLYKGFLS EEC HLI L                                   
Sbjct: 61  LSWTPRAFLYKGFLSDEECDHLIKLAKGKLEKSMVVADVDSGESEDSEVRTSSGMFLTKR 120

Query: 128 -DDIVAGIEAKIAAWTMRLVKSRNCSFGSDNGEPIQILRYENGQQYVPHFDFFQDPVNVA 187
            DDIVA +EAK+AAWT             +NGE +QIL YENGQ+Y PHFD+F D   + 
Sbjct: 121 QDDIVANVEAKLAAWTF---------LPEENGEALQILHYENGQKYDPHFDYFYDKKALE 180

Query: 188 AGGHRIATVLMYLSNVERGGETVFPD----------------------SPPKKGDALLFF 247
            GGHRIATVLMYLSNV +GGETVFP+                        P+KGDALLFF
Sbjct: 181 LGGHRIATVLMYLSNVTKGGETVFPNWKGKTPQLKDDSWSKCAKQGYAVKPRKGDALLFF 240

Query: 248 SLHPNVTTDPTSYHGSCPVIEGEKWSATKWIHMLPVDEIWRNPDCVDENEHCSAWAKAGE 279
           +LH N TTDP S HGSCPVIEGEKWSAT+WIH+    +  +   CVD++E C  WA AGE
Sbjct: 241 NLHLNGTTDPNSLHGSCPVIEGEKWSATRWIHVRSFGK--KKLVCVDDHESCQEWADAGE 288

[Close]
BLAST of Cp4.1LG04g01360 vs. TAIR 10
Match: AT5G18900.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 251.5 bits (641), Expect = 7.9e-67
Identity = 131/287 (45.64%), Postives = 164/287 (57.14%), Query Frame = 0 

Query: 53  MDGSSIKIDPTRVVQLSSQPRAFLYKGFLSAEECQHLIDL-------------------- 112
           +  SS+ ++P++V Q+SS+PRAF+Y+GFL+  EC H++ L                    
Sbjct: 25  ISSSSVFVNPSKVKQVSSKPRAFVYEGFLTELECDHMVSLAKASLKRSAVADNDSGESKF 84

Query: 113 ---------------DDIVAGIEAKIAAWTMRLVKSRNCSFGSDNGEPIQILRYENGQQY 172
                          D IV+GIE KI+ WT             +NGE IQ+LRYE+GQ+Y
Sbjct: 85  SEVRTSSGTFISKGKDPIVSGIEDKISTWTF---------LPKENGEDIQVLRYEHGQKY 144

Query: 173 VPHFDFFQDPVNVAAGGHRIATVLMYLSNVERGGETVFPDS------------------- 232
             HFD+F D VN+  GGHR+AT+LMYLSNV +GGETVFPD+                   
Sbjct: 145 DAHFDYFHDKVNIVRGGHRMATILMYLSNVTKGGETVFPDAEIPSRRVLSENKEDLSDCA 204

Query: 233 ------PPKKGDALLFFSLHPNVTTDPTSYHGSCPVIEGEKWSATKWIHMLPVDEI-WRN 279
                  P+KGDALLFF+LHP+   DP S HG CPVIEGEKWSATKWIH+   D I   +
Sbjct: 205 KRGIAVKPRKGDALLFFNLHPDAIPDPLSLHGGCPVIEGEKWSATKWIHVDSFDRIVTPS 264

[Close]
BLAST of Cp4.1LG04g01360 vs. TAIR 10
Match: AT3G06300.1 (P4H isoform 2 )

HSP 1 Score: 239.6 bits (610), Expect = 3.1e-63
Identity = 129/280 (46.07%), Postives = 158/280 (56.43%), Query Frame = 0 

Query: 60  IDPTRVVQLSSQPRAFLYKGFLSAEECQHLIDL--------------------------- 119
           I+P++V Q+SS+PRAF+Y+GFL+  EC HLI L                           
Sbjct: 33  INPSKVKQVSSKPRAFVYEGFLTDLECDHLISLAKENLQRSAVADNDNGESQVSDVRTSS 92

Query: 120 --------DDIVAGIEAKIAAWTMRLVKSRNCSFGSDNGEPIQILRYENGQQYVPHFDFF 179
                   D IV+GIE K++ WT             +NGE +Q+LRYE+GQ+Y  HFD+F
Sbjct: 93  GTFISKGKDPIVSGIEDKLSTWTF---------LPKENGEDLQVLRYEHGQKYDAHFDYF 152

Query: 180 QDPVNVAAGGHRIATVLMYLSNVERGGETVFPDS-------------------------P 239
            D VN+A GGHRIATVL+YLSNV +GGETVFPD+                          
Sbjct: 153 HDKVNIARGGHRIATVLLYLSNVTKGGETVFPDAQEFSRRSLSENKDDLSDCAKKGIAVK 212

Query: 240 PKKGDALLFFSLHPNVTTDPTSYHGSCPVIEGEKWSATKWIHMLPVDEI-WRNPDCVDEN 279
           PKKG+ALLFF+L  +   DP S HG CPVIEGEKWSATKWIH+   D+I   + +C D N
Sbjct: 213 PKKGNALLFFNLQQDAIPDPFSLHGGCPVIEGEKWSATKWIHVDSFDKILTHDGNCTDVN 272

The following BLAST results are available for this feature:
BLAST of Cp4.1LG04g01360 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q8L9704.5e-8350.91Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=... [more]
F4J0A88.8e-7146.50Probable prolyl 4-hydroxylase 6 OS=Arabidopsis thaliana OX=3702 GN=P4H6 PE=2 SV=... [more]
Q8LAN31.1e-6545.64Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=... [more]
F4JAU34.4e-6246.07Prolyl 4-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=P4H2 PE=1 SV=1[more]
Q8GXT74.5e-4336.75Probable prolyl 4-hydroxylase 12 OS=Arabidopsis thaliana OX=3702 GN=P4H12 PE=2 S... [more]
BLAST of Cp4.1LG04g01360 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_023530715.12.36e-18279.22probable prolyl 4-hydroxylase 7 [Cucurbita pepo subsp. pepo] >XP_023530716.1 pro... [more]
XP_022931100.19.61e-18278.92probable prolyl 4-hydroxylase 7 [Cucurbita moschata][more]
XP_022971148.11.08e-18078.25probable prolyl 4-hydroxylase 7 [Cucurbita maxima] >XP_022971154.1 probable prol... [more]
KAG6588394.11.78e-17677.51putative prolyl 4-hydroxylase 7, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7022241.13.01e-17083.97putative prolyl 4-hydroxylase 7 [Cucurbita argyrosperma subsp. argyrosperma][more]
BLAST of Cp4.1LG04g01360 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1EYJ14.65e-18278.92Procollagen-proline 4-dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111437385 ... [more]
A0A6J1I5Z95.23e-18178.25Procollagen-proline 4-dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111469906 PE... [more]
A0A1S3B8142.65e-13160.42Procollagen-proline 4-dioxygenase OS=Cucumis melo OX=3656 GN=LOC103487037 PE=3 S... [more]
A0A0A0LG324.52e-13059.82Procollagen-proline 4-dioxygenase OS=Cucumis sativus OX=3659 GN=Csa_3G828960 PE=... [more]
A0A6J1DTY45.12e-13060.60Procollagen-proline 4-dioxygenase OS=Momordica charantia OX=3673 GN=LOC111024321... [more]
BLAST of Cp4.1LG04g01360 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G28480.13.2e-8450.91Oxoglutarate/iron-dependent oxygenase [more]
AT3G28480.22.1e-8349.41Oxoglutarate/iron-dependent oxygenase [more]
AT3G28490.16.2e-7246.50Oxoglutarate/iron-dependent oxygenase [more]
AT5G18900.17.9e-6745.642-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G06300.13.1e-6346.07P4H isoform 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003582ShKT domainSMARTSM00254ShkT_1coord: 233..279
e-value: 3.4E-6
score: 36.6
IPR003582ShKT domainPFAMPF01549ShKcoord: 234..257
e-value: 3.6E-4
score: 21.0
IPR006620Prolyl 4-hydroxylase, alpha subunitSMARTSM00702p4hccoord: 72..221
e-value: 4.5E-34
score: 129.2
IPR044862Prolyl 4-hydroxylase alpha subunit, Fe(2+) 2OG dioxygenase domainPFAMPF136402OG-FeII_Oxy_3coord: 127..221
e-value: 3.2E-20
score: 72.8
NoneNo IPR availableGENE3D2.60.120.620q2cbj1_9rhob like domaincoord: 57..222
e-value: 1.9E-48
score: 167.0
NoneNo IPR availablePANTHERPTHR10869:SF140OS03G0803500 PROTEINcoord: 53..92
coord: 93..178
NoneNo IPR availablePANTHERPTHR10869:SF140OS03G0803500 PROTEINcoord: 180..279
IPR045054Prolyl 4-hydroxylasePANTHERPTHR10869PROLYL 4-HYDROXYLASE ALPHA SUBUNITcoord: 180..279
IPR045054Prolyl 4-hydroxylasePANTHERPTHR10869PROLYL 4-HYDROXYLASE ALPHA SUBUNITcoord: 53..92
coord: 93..178
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 122..222
score: 12.193467

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG04g01360.1Cp4.1LG04g01360.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019511 peptidyl-proline hydroxylation
cellular_component GO:0005789 endoplasmic reticulum membrane
molecular_function GO:0005506 iron ion binding
molecular_function GO:0031418 L-ascorbic acid binding
molecular_function GO:0004656 procollagen-proline 4-dioxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen