Cp4.1LG03g13440 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG03g13440
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionEXPERA domain-containing protein
LocationCp4.1LG03: 9560202 .. 9563347 (-)
RNA-Seq ExpressionCp4.1LG03g13440
SyntenyCp4.1LG03g13440
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAATGGTGGGAGTGGTTTCAGACTGGACAGGGAGTTCATTTGGTCAAACTTGAAACCAGAGGAAAAAGAAAGTCGCCAATGGCGGGGGAATCTGAAGCTGCTCGGCATCCCTACGCACCCACAGATCTGGAACTCCCCGGATATGTTCCTATTTCACTACCCCTCACTACTATTCTCGGCGTTTATCTCCTTTCTTCTCTCTTTATCACTTTGCTGCTCTGGACTCTTTCCGGTATGCTTTCATCTCTTACTTTGATATTTACTGTCGTTTTTTGGATTATGTTTTATGCGCTGCGAATTCTGCTTGATTTCTTGCTGGAGAAGAAGACCGATGGTTTTCATAATCCGTAGTTTGTTGTTGTATTGGATTTCTGTCCACTTGTTAGAGGTCACTTGTTGAGTGAAGTAATTGGAAGCAAAATCAAACAATTTCAAATATTCTATGTTTTTCGTGTCCTCCAAGAATTCTATTGAATGTCTTTGCTGAATCCGAAGGCTGGCGGTGAAACCGATGTAAAAATTCAAATCTGTGTCTCGTTCAATTATTCTTTTCGAGAGGATCGAGATCTGTTAAACGGATAGACGCCTTGCCTTCCTACTCCTTAACTTGTGCTATTGTCTTTTGGTTTAGATGATTGCTTTAGAAAGGGAATACTGATGGGACGGACTAAGCGAGGATGTCTTTGATTATACCTGAGTGTGAAAGAATGAAACATGTAGCAAACTGAAATCTATTTGGCCATTTATGGTTCTGTCAATCCGCGTTTGACTTCATTTGATTGGTTAATTTTGATACGATACAATATCTATTTTAGATTTAAACTCTTGATATTTCCTTTGGTTATGCATACTCCCCTTTCTCTAAACAATCATTCATCCATACACTTTCTACTAATGATGAACATAAATTTATCTAGATTTCAGAAATGCTATGAGGATGAAATGTTAATGCTAAGATTACTATTTGGATATTCGTGATTAGCTTGTAAGTTTGTTTGTGTTGAGTGGTTATGGATATAGTGGGTTGAGAGTGAGAGAGTGAGAGAGAGGCAGATTTGTATAGTTTTGGAAAGAGTCCTAGGCCTCTCGTAGAGCTTGGAACCTTGTAATTTGTCTAATATCAATATAATATTTCAATTAACCTGTCCAATTTTTGGTTCCTATCAAATTAGTTCCACTACATTTGTGCTCGAAATTGAATCTTTTCAAGTCTAGAAGAATATTGATGAAGATGTTGTCCACAAATGGATCTTTTTCTCTTTAGTGCAGATAGTAAGGATGCTCATTTTGGGGTCCTTGTCCTTCGAAGTGTTTTTTCTTGTAGCTTGAGCAATCCCTCCCCAATGGTTCCACTTTTTTCCTTAGTGTAGGTAGAATGTTGTACAAACACTAGATCCTAACACCATGATTGAAGTATGAAACACCTGATAATGGGCACTATAATCGAACACTTAGTGTAGGTAGAGTGTTGTATAAGCACTTATGTTGCAAGCTAGGACATAGTTTGCTTTCCTATTGCACCCTGTGTTTTTTTCCTATTGAAGTATGAAACACCTGATAATGGGCACTATATTCAAACACTCAGTGTAGGTAGAGTATTCTACAAGCACATATTTTCCTTAGTGTAGGTAGAGTGTTGTACAAGCACTTATGTTGCAAGCTATGACATATTTTGCTTTCCTATTGCACCCTGTGTTTTTTTCCTATTGAAGTATGAAACACCTGATAATGGGCACTATATTCAAACACTCAGTGTAGGTAGAGTATTCTACAAGCACATATTTTCCTTAGTGTAGGTAGAGTGTTGTACAAGCACTTATGTTGCAAGCTATGACATATTTTGCTTTCCTATTGCACCCTGTGTTTTTTTTATGTTCCCTTCCGTGGTGTTTGTACACGTGTTCTCTCCATCTTTATCTATTTCTTTTTATCTGAATTTAGTAATGGAAAAAACTGTAGTTCTTAGAGCTGTTTCGAAAAGGTTCTATGCTTGTTGCTTTATCTTCATTTCCCCCTTATAACAGGACGACTTCCCAAAAAAACAAAGATTGAGAGGCTGCTCATGTGCTGGTGGATATTTACGGGCCTTACCCACATTGTTCTCGAGGGTTACTTTGTTTTCTCTCCAGAATTTTACAAGGACAGTTCTTACCTTGCTGAAGTTTGTAAGTTAATTTAATTCACTTTCTTTGTTACCCTAGCTGTGGTACTGGAAGGGCTGTATGGATGCTCCTCTAATAATGTTCATGTGCACTACTTACCAGGGAAAGAATACAGCAAGGGAGATTCTCGATACGCAACTCGGGACTCTGCGATTGTCAGTCTTGAAGGACTGACTGCTGTTTTAGAGGGTCCAGCTTGCATCTTAGCAGTGTAAGAATTTATCATCTGTGTGGTTTTGGGAGAGAATAGTTAATCAATTTCTAAATAACTAAGATACAAGAAGTTATGATACAAAGAAAATACAAAAGAGGTAACACTAGCTAATAAAATCTAAACTAAAGAATTTTGAAATGCTATACATATAAGGAATTACATTTGAACTTTCTGTTCATAATTAGCCATTCATCAACAAATTTGTTGGGCTGTGTATAATTCGCAGGTATGCAATAGCTACAGACAAGTCGTACAGTTACATACTGCAGTTCGCCATATCATTGGGACAGCTATATGGAACTTTCATGTACTACATAACTGCTCTGTTGGATGGTGATAATTTTTCTTCATCCCCAATTTATTACTATTTATACTACGTATTGTTAAATTCCTTCTGGATTCTTATTCCTTCGCTTATCGCTATCCGGTGTTGGAAGAAAATTTGTATTGCTATGAGCAGTGAAAGCCACAAGAAAACCAAAGTTCCCTAAGAAGTGATCCATATTATGACGTCTACCCCTTTGGATCCATGGAAAATAATAGATTAGATAGTACACAAGATTCTTGTTGTATTGTAATCTAAGTTATTGTCGTGTGACATTCAGAAAACGAGACACTCGTGAAATTTGAACATTTACACGAAGTTTGGGTTTGTTGATTATGCTTTCTTGGGGTGTTAAATTGACGATGTCGATTCGAGTTTGTTGTAGGTTATTTCATCATCTTTTTAGCGTAAAGGTAAAGCATTTCTTAGATATTTGCTATTGTAT

mRNA sequence

TAATGGTGGGAGTGGTTTCAGACTGGACAGGGAGTTCATTTGGTCAAACTTGAAACCAGAGGAAAAAGAAAGTCGCCAATGGCGGGGGAATCTGAAGCTGCTCGGCATCCCTACGCACCCACAGATCTGGAACTCCCCGGATATGTTCCTATTTCACTACCCCTCACTACTATTCTCGGCGTTTATCTCCTTTCTTCTCTCTTTATCACTTTGCTGCTCTGGACTCTTTCCGGACGACTTCCCAAAAAAACAAAGATTGAGAGGCTGCTCATGTGCTGGTGGATATTTACGGGCCTTACCCACATTGTTCTCGAGGGTTACTTTGTTTTCTCTCCAGAATTTTACAAGGACAGTTCTTACCTTGCTGAAGTTTGGAAAGAATACAGCAAGGGAGATTCTCGATACGCAACTCGGGACTCTGCGATTGTCAGTCTTGAAGGACTGACTGCTGTTTTAGAGGGTCCAGCTTGCATCTTAGCAGTGTATGCAATAGCTACAGACAAGTCGTACAGTTACATACTGCAGTTCGCCATATCATTGGGACAGCTATATGGAACTTTCATGTACTACATAACTGCTCTGTTGGATGGTGATAATTTTTCTTCATCCCCAATTTATTACTATTTATACTACGTATTGTTAAATTCCTTCTGGATTCTTATTCCTTCGCTTATCGCTATCCGGTGTTGGAAGAAAATTTGTATTGCTATGAGCAGTGAAAGCCACAAGAAAACCAAAGTTCCCTAAGAAGTGATCCATATTATGACGTCTACCCCTTTGGATCCATGGAAAATAATAGATTAGATAGTACACAAGATTCTTGTTGTATTGTAATCTAAGTTATTGTCGTGTGACATTCAGAAAACGAGACACTCGTGAAATTTGAACATTTACACGAAGTTTGGGTTTGTTGATTATGCTTTCTTGGGGTGTTAAATTGACGATGTCGATTCGAGTTTGTTGTAGGTTATTTCATCATCTTTTTAGCGTAAAGGTAAAGCATTTCTTAGATATTTGCTATTGTAT

Coding sequence (CDS)

ATGGCGGGGGAATCTGAAGCTGCTCGGCATCCCTACGCACCCACAGATCTGGAACTCCCCGGATATGTTCCTATTTCACTACCCCTCACTACTATTCTCGGCGTTTATCTCCTTTCTTCTCTCTTTATCACTTTGCTGCTCTGGACTCTTTCCGGACGACTTCCCAAAAAAACAAAGATTGAGAGGCTGCTCATGTGCTGGTGGATATTTACGGGCCTTACCCACATTGTTCTCGAGGGTTACTTTGTTTTCTCTCCAGAATTTTACAAGGACAGTTCTTACCTTGCTGAAGTTTGGAAAGAATACAGCAAGGGAGATTCTCGATACGCAACTCGGGACTCTGCGATTGTCAGTCTTGAAGGACTGACTGCTGTTTTAGAGGGTCCAGCTTGCATCTTAGCAGTGTATGCAATAGCTACAGACAAGTCGTACAGTTACATACTGCAGTTCGCCATATCATTGGGACAGCTATATGGAACTTTCATGTACTACATAACTGCTCTGTTGGATGGTGATAATTTTTCTTCATCCCCAATTTATTACTATTTATACTACGTATTGTTAAATTCCTTCTGGATTCTTATTCCTTCGCTTATCGCTATCCGGTGTTGGAAGAAAATTTGTATTGCTATGAGCAGTGAAAGCCACAAGAAAACCAAAGTTCCCTAA

Protein sequence

MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYLLSSLFITLLLWTLSGRLPKKTKIERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDSSYLAEVWKEYSKGDSRYATRDSAIVSLEGLTAVLEGPACILAVYAIATDKSYSYILQFAISLGQLYGTFMYYITALLDGDNFSSSPIYYYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP
Homology
BLAST of Cp4.1LG03g13440 vs. ExPASy Swiss-Prot
Match: O48962 (Probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Arabidopsis thaliana OX=3702 GN=At1g20050 PE=2 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 1.0e-74
Identity = 138/224 (61.61%), Postives = 178/224 (79.46%), Query Frame = 0

Query: 6   EAARHPYAPTDLELPGYVPISLPLTTILGVYLLSSLFITLLLWTLSGRLPKKTKIERLLM 65
           E   HPY P DL LPGYVPIS+ +++I+ +YL SSL +  L+W L GR  KK K+++LLM
Sbjct: 2   EELAHPYVPRDLNLPGYVPISMSMSSIVSIYLGSSLLVVSLVWLLFGR--KKAKLDKLLM 61

Query: 66  CWWIFTGLTHIVLEGYFVFSPEFYKD--SSYLAEVWKEYSKGDSRYATRDSAIVSLEGLT 125
           CWW FTGLTH++LEGYFVFSPEF+KD  S+YLAEVWKEYSKGDSRY  RDSA+VS+EG+T
Sbjct: 62  CWWTFTGLTHVILEGYFVFSPEFFKDNTSAYLAEVWKEYSKGDSRYVGRDSAVVSVEGIT 121

Query: 126 AVLEGPACILAVYAIATDKSYSYILQFAISLGQLYGTFMYYITALLDGDNFSSSPIYYYL 185
           AV+ GPA +LA+YAIA +KSYSY+LQ AIS+ QLYG  +Y+ITA+L+GDNF+++  YYY 
Sbjct: 122 AVIVGPASLLAIYAIAKEKSYSYVLQLAISVCQLYGCLVYFITAILEGDNFATNSFYYYS 181

Query: 186 YYVLLNSFWILIPSLIAIRCWKKICIA-------MSSESHKKTK 221
           YY+  N +W+LIPSLI+ RCWKKIC A       + +++ KKT+
Sbjct: 182 YYIGANCWWVLIPSLISFRCWKKICAAAAIANNNVETKTKKKTR 223

BLAST of Cp4.1LG03g13440 vs. ExPASy Swiss-Prot
Match: Q9FTZ2 (Probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0103600 PE=2 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 4.3e-65
Identity = 124/211 (58.77%), Postives = 159/211 (75.36%), Query Frame = 0

Query: 10  HPYAPTDLELPGYVPISLPLTTILGVYLLSSLFITLLLWTLSGRLPKK-TKIERLLMCWW 69
           HPYAP +L LPG+VP+ L    IL  YL +SLF+ L +W +SGR  ++ +  +R LMCWW
Sbjct: 7   HPYAPAELHLPGFVPLQLSQAQILVPYLATSLFLLLAVWLISGRCSRRLSDTDRWLMCWW 66

Query: 70  IFTGLTHIVLEGYFVFSPEFY--KDSSYLAEVWKEYSKGDSRYATRDSAIVSLEGLTAVL 129
            FTGLTHI++EG FVF+P F+  ++ SY  EVWKEYSKGDSRY  RD A V++EG+TAVL
Sbjct: 67  AFTGLTHIIIEGTFVFAPNFFSNQNPSYFDEVWKEYSKGDSRYVARDPATVTVEGITAVL 126

Query: 130 EGPACILAVYAIATDKSYSYILQFAISLGQLYGTFMYYITALLDGDNFSSSPIYYYLYYV 189
           EGPA +LAVYAIA+ KSYS+ILQF + LGQLYG  +Y+ITA LDG NF +SP Y++ Y++
Sbjct: 127 EGPASLLAVYAIASGKSYSHILQFTVCLGQLYGCLVYFITAYLDGFNFWTSPFYFWAYFI 186

Query: 190 LLNSFWILIPSLIAIRCWKKICIAMSSESHK 218
             NS W++IP++IAIR WKKIC A   E  K
Sbjct: 187 GANSSWVVIPTMIAIRSWKKICAAFQGEKVK 217

BLAST of Cp4.1LG03g13440 vs. ExPASy Swiss-Prot
Match: Q9JJ46 (3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Rattus norvegicus OX=10116 GN=Ebp PE=1 SV=3)

HSP 1 Score: 137.5 bits (345), Expect = 1.8e-31
Identity = 81/219 (36.99%), Postives = 126/219 (57.53%), Query Frame = 0

Query: 10  HPYAPTDLELPGYVPISLPLTTIL-GVYLLSSLFITLLLWTLSGR---LPKKTKIERLLM 69
           HPY P  L L  +VP  LP   IL G++  S + I ++ W LS R   +P  T   RL +
Sbjct: 9   HPYWPRHLRLDNFVPNDLPTWHILVGLFSFSGVLI-VITWLLSSRVSVVPLGTG-RRLAL 68

Query: 70  CWWIFTGLTHIVLEGYFVFSPE-FYKDSSYLAEVWKEYSKGDSRYATRDSAIVSLEGLTA 129
           CW+      H+V+EG+F F  E   +D ++L+++WKEYSKGDSRY   D  IV +E +TA
Sbjct: 69  CWFAVCTFIHLVIEGWFSFYHEILLEDQAFLSQLWKEYSKGDSRYILSDGFIVCMESVTA 128

Query: 130 VLEGPACILAVYAIATDKSYSYILQFAISLGQLYGTFMYYITALLDGDNFS--SSPIYYY 189
            L GP  +  V A    + + ++LQ  +S+GQ+YG  +Y++T L DG        P+Y++
Sbjct: 129 CLWGPLSLWVVIAFLRHQPFRFVLQLVVSVGQIYGDVLYFLTELRDGFQHGELGHPLYFW 188

Query: 190 LYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKV 222
            Y+V++N+ W++IP ++     K +  A S   +K  K+
Sbjct: 189 FYFVIMNAIWLVIPGILVFDAIKHLTNAQSMLDNKVMKI 225

BLAST of Cp4.1LG03g13440 vs. ExPASy Swiss-Prot
Match: P70245 (3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Mus musculus OX=10090 GN=Ebp PE=1 SV=3)

HSP 1 Score: 134.4 bits (337), Expect = 1.6e-30
Identity = 78/218 (35.78%), Postives = 128/218 (58.72%), Query Frame = 0

Query: 10  HPYAPTDLELPGYVPISLPLTTIL-GVYLLSSLFITLLLWTLSGR--LPKKTKIERLLMC 69
           HPY P  L+L  +VP  LP + IL G++ +S   I ++ W LS R  +       RL +C
Sbjct: 9   HPYWPRHLKLDNFVPNDLPTSHILVGLFSISGGLI-VITWLLSSRASVVPLGAGRRLALC 68

Query: 70  WWIFTGLTHIVLEGYF-VFSPEFYKDSSYLAEVWKEYSKGDSRYATRDSAIVSLEGLTAV 129
           W+      H+V+EG+F +++    +D ++L+++WKEYSKGDSRY   DS +V +E +TA 
Sbjct: 69  WFAVCTFIHLVIEGWFSLYNGILLEDQAFLSQLWKEYSKGDSRYILSDSFVVCMETVTAC 128

Query: 130 LEGPACILAVYAIATDKSYSYILQFAISLGQLYGTFMYYITALLDGDNFS--SSPIYYYL 189
           L GP  +  V A    + + ++LQ  +S+GQ+YG  +Y++T L +G        P+Y++ 
Sbjct: 129 LWGPLSLWVVIAFLRQQPFRFVLQLVVSMGQIYGDVLYFLTELHEGLQHGEIGHPVYFWF 188

Query: 190 YYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKV 222
           Y+V LN+ W++IPS++ +   K +  A S    K  K+
Sbjct: 189 YFVFLNAVWLVIPSILVLDAIKHLTSAQSVLDSKVMKI 225

BLAST of Cp4.1LG03g13440 vs. ExPASy Swiss-Prot
Match: Q60490 (3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Cavia porcellus OX=10141 GN=EBP PE=1 SV=3)

HSP 1 Score: 128.6 bits (322), Expect = 8.5e-29
Identity = 76/205 (37.07%), Postives = 112/205 (54.63%), Query Frame = 0

Query: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYLLSSLFITLLLWTLSGR---LPKK 60
           MA  S    HPY P  L L  +VP  L    I+ V       + + +W LS R   +P  
Sbjct: 1   MATTSTGPLHPYWPRHLRLDHFVPNDLSAWYIVTVLFTVFGALVVTMWLLSSRASVVPLG 60

Query: 61  TKIERLLMCWWIFTGLTHIVLEGYFV-FSPEFYKDSSYLAEVWKEYSKGDSRYATRDSAI 120
           T   RL +CW+      H+V+EG+FV +      D ++L+++WKEY+KGDSRY   D+ I
Sbjct: 61  T-WRRLSVCWFAVCAFVHLVIEGWFVLYQKAILGDQAFLSQLWKEYAKGDSRYIIEDNFI 120

Query: 121 VSLEGLTAVLEGPACILAVYAIATDKSYSYILQFAISLGQLYGTFMYYITALLDGDNFS- 180
           + +E +T VL GP  + AV A        Y+LQF ISLGQ+YG  +Y++T   DG     
Sbjct: 121 ICMESITVVLWGPLSLWAVIAFLRQHPSRYVLQFVISLGQIYGDLLYFLTEYRDGFQHGE 180

Query: 181 -SSPIYYYLYYVLLNSFWILIPSLI 200
              PIY++ Y+  +N  W++IP ++
Sbjct: 181 MGHPIYFWFYFFFMNVLWLVIPGVL 204

BLAST of Cp4.1LG03g13440 vs. NCBI nr
Match: XP_023526321.1 (probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 438 bits (1126), Expect = 7.97e-155
Identity = 222/222 (100.00%), Postives = 222/222 (100.00%), Query Frame = 0

Query: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYLLSSLFITLLLWTLSGRLPKKTKI 60
           MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYLLSSLFITLLLWTLSGRLPKKTKI
Sbjct: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYLLSSLFITLLLWTLSGRLPKKTKI 60

Query: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDSSYLAEVWKEYSKGDSRYATRDSAIVSLE 120
           ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDSSYLAEVWKEYSKGDSRYATRDSAIVSLE
Sbjct: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDSSYLAEVWKEYSKGDSRYATRDSAIVSLE 120

Query: 121 GLTAVLEGPACILAVYAIATDKSYSYILQFAISLGQLYGTFMYYITALLDGDNFSSSPIY 180
           GLTAVLEGPACILAVYAIATDKSYSYILQFAISLGQLYGTFMYYITALLDGDNFSSSPIY
Sbjct: 121 GLTAVLEGPACILAVYAIATDKSYSYILQFAISLGQLYGTFMYYITALLDGDNFSSSPIY 180

Query: 181 YYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP 222
           YYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP
Sbjct: 181 YYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP 222

BLAST of Cp4.1LG03g13440 vs. NCBI nr
Match: KAG6581969.1 (putative 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018388.1 putative 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 436 bits (1122), Expect = 3.25e-154
Identity = 220/222 (99.10%), Postives = 222/222 (100.00%), Query Frame = 0

Query: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYLLSSLFITLLLWTLSGRLPKKTKI 60
           MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVY+LSSLFITLLLWTLSGRLPKKTKI
Sbjct: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGRLPKKTKI 60

Query: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDSSYLAEVWKEYSKGDSRYATRDSAIVSLE 120
           ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDSSYLAEVWKEYSKGDSRYATRDSAIVSLE
Sbjct: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDSSYLAEVWKEYSKGDSRYATRDSAIVSLE 120

Query: 121 GLTAVLEGPACILAVYAIATDKSYSYILQFAISLGQLYGTFMYYITALLDGDNFSSSPIY 180
           GLTAVLEGPACILAVYAIATDKSYSY+LQFAISLGQLYGTFMYYITALLDGDNFSSSPIY
Sbjct: 121 GLTAVLEGPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFSSSPIY 180

Query: 181 YYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP 222
           YYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP
Sbjct: 181 YYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP 222

BLAST of Cp4.1LG03g13440 vs. NCBI nr
Match: XP_022955667.1 (probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Cucurbita moschata])

HSP 1 Score: 435 bits (1118), Expect = 1.32e-153
Identity = 219/222 (98.65%), Postives = 221/222 (99.55%), Query Frame = 0

Query: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYLLSSLFITLLLWTLSGRLPKKTKI 60
           MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVY+LSSLFITLLLWTLSGRLPKKTKI
Sbjct: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGRLPKKTKI 60

Query: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDSSYLAEVWKEYSKGDSRYATRDSAIVSLE 120
           ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKD SYLAEVWKEYSKGDSRYATRDSAIVSLE
Sbjct: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDGSYLAEVWKEYSKGDSRYATRDSAIVSLE 120

Query: 121 GLTAVLEGPACILAVYAIATDKSYSYILQFAISLGQLYGTFMYYITALLDGDNFSSSPIY 180
           GLTAVLEGPACILAVYAIATDKSYSY+LQFAISLGQLYGTFMYYITALLDGDNFSSSPIY
Sbjct: 121 GLTAVLEGPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFSSSPIY 180

Query: 181 YYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP 222
           YYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP
Sbjct: 181 YYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP 222

BLAST of Cp4.1LG03g13440 vs. NCBI nr
Match: XP_022980063.1 (probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Cucurbita maxima])

HSP 1 Score: 434 bits (1117), Expect = 1.88e-153
Identity = 220/222 (99.10%), Postives = 221/222 (99.55%), Query Frame = 0

Query: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYLLSSLFITLLLWTLSGRLPKKTKI 60
           MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYLLSSLFITLLLWTLSGRLPKKTKI
Sbjct: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYLLSSLFITLLLWTLSGRLPKKTKI 60

Query: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDSSYLAEVWKEYSKGDSRYATRDSAIVSLE 120
           ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDSSYLAEVWKEYSKGDSRYA R+SAIVSLE
Sbjct: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDSSYLAEVWKEYSKGDSRYAARESAIVSLE 120

Query: 121 GLTAVLEGPACILAVYAIATDKSYSYILQFAISLGQLYGTFMYYITALLDGDNFSSSPIY 180
           GLTAVLEGPACILAVYAIATDKSYSYILQFAISLGQLYGTFMYYITALLDGDNFSSSPIY
Sbjct: 121 GLTAVLEGPACILAVYAIATDKSYSYILQFAISLGQLYGTFMYYITALLDGDNFSSSPIY 180

Query: 181 YYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP 222
           YYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP
Sbjct: 181 YYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP 222

BLAST of Cp4.1LG03g13440 vs. NCBI nr
Match: XP_038881035.1 (probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Benincasa hispida])

HSP 1 Score: 383 bits (984), Expect = 3.79e-133
Identity = 192/224 (85.71%), Postives = 207/224 (92.41%), Query Frame = 0

Query: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYLLSSLFITLLLWTLSGRLPKKTKI 60
           MAGESEAARHPY PTDL+LP YVPISLP++TIL VYLLSSLF+T L+WTLSGRL K+ KI
Sbjct: 1   MAGESEAARHPYIPTDLKLPEYVPISLPVSTILSVYLLSSLFVTFLVWTLSGRLTKRAKI 60

Query: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKD--SSYLAEVWKEYSKGDSRYATRDSAIVS 120
           ERLLMCWWIFTGLTH+VLEGYFVFSPEFYKD  S YLAEVWKEYSKGDSRYA R+S IVS
Sbjct: 61  ERLLMCWWIFTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS 120

Query: 121 LEGLTAVLEGPACILAVYAIATDKSYSYILQFAISLGQLYGTFMYYITALLDGDNFSSSP 180
           +EGLT+VLEGPACILA YAIAT+KSYSYILQFAISLGQLYGTF+YYITALLDGDNFSS P
Sbjct: 121 IEGLTSVLEGPACILAAYAIATEKSYSYILQFAISLGQLYGTFVYYITALLDGDNFSSGP 180

Query: 181 IYYYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP 222
           IYYY Y+V+ NSFWILIPSLIA RCWKKICIAMS+ESH KTKVP
Sbjct: 181 IYYYSYFVVANSFWILIPSLIATRCWKKICIAMSTESHMKTKVP 224

BLAST of Cp4.1LG03g13440 vs. ExPASy TrEMBL
Match: A0A6J1GU98 (probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Cucurbita moschata OX=3662 GN=LOC111457598 PE=3 SV=1)

HSP 1 Score: 435 bits (1118), Expect = 6.40e-154
Identity = 219/222 (98.65%), Postives = 221/222 (99.55%), Query Frame = 0

Query: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYLLSSLFITLLLWTLSGRLPKKTKI 60
           MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVY+LSSLFITLLLWTLSGRLPKKTKI
Sbjct: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGRLPKKTKI 60

Query: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDSSYLAEVWKEYSKGDSRYATRDSAIVSLE 120
           ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKD SYLAEVWKEYSKGDSRYATRDSAIVSLE
Sbjct: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDGSYLAEVWKEYSKGDSRYATRDSAIVSLE 120

Query: 121 GLTAVLEGPACILAVYAIATDKSYSYILQFAISLGQLYGTFMYYITALLDGDNFSSSPIY 180
           GLTAVLEGPACILAVYAIATDKSYSY+LQFAISLGQLYGTFMYYITALLDGDNFSSSPIY
Sbjct: 121 GLTAVLEGPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFSSSPIY 180

Query: 181 YYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP 222
           YYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP
Sbjct: 181 YYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP 222

BLAST of Cp4.1LG03g13440 vs. ExPASy TrEMBL
Match: A0A6J1IY56 (probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Cucurbita maxima OX=3661 GN=LOC111479580 PE=3 SV=1)

HSP 1 Score: 434 bits (1117), Expect = 9.10e-154
Identity = 220/222 (99.10%), Postives = 221/222 (99.55%), Query Frame = 0

Query: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYLLSSLFITLLLWTLSGRLPKKTKI 60
           MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYLLSSLFITLLLWTLSGRLPKKTKI
Sbjct: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYLLSSLFITLLLWTLSGRLPKKTKI 60

Query: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDSSYLAEVWKEYSKGDSRYATRDSAIVSLE 120
           ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDSSYLAEVWKEYSKGDSRYA R+SAIVSLE
Sbjct: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDSSYLAEVWKEYSKGDSRYAARESAIVSLE 120

Query: 121 GLTAVLEGPACILAVYAIATDKSYSYILQFAISLGQLYGTFMYYITALLDGDNFSSSPIY 180
           GLTAVLEGPACILAVYAIATDKSYSYILQFAISLGQLYGTFMYYITALLDGDNFSSSPIY
Sbjct: 121 GLTAVLEGPACILAVYAIATDKSYSYILQFAISLGQLYGTFMYYITALLDGDNFSSSPIY 180

Query: 181 YYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP 222
           YYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP
Sbjct: 181 YYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKVP 222

BLAST of Cp4.1LG03g13440 vs. ExPASy TrEMBL
Match: A0A6J1E0U0 (probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Momordica charantia OX=3673 GN=LOC111024900 PE=3 SV=1)

HSP 1 Score: 369 bits (947), Expect = 7.96e-128
Identity = 180/223 (80.72%), Postives = 201/223 (90.13%), Query Frame = 0

Query: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYLLSSLFITLLLWTLSGRLPKKTKI 60
           MAG+SEAA HPY P DL+L  YVP+SLP +TI+GVY LSS+ +T L+WTLSGRLPKK KI
Sbjct: 1   MAGKSEAAPHPYVPADLQLSEYVPVSLPFSTIIGVYFLSSILVTSLVWTLSGRLPKKAKI 60

Query: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKD--SSYLAEVWKEYSKGDSRYATRDSAIVS 120
           ERLLMCWW+FTGLTH++LEGYFVFSPEFYKD  S YLAEVWKEYSKGDSRYA R+S IVS
Sbjct: 61  ERLLMCWWVFTGLTHMILEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS 120

Query: 121 LEGLTAVLEGPACILAVYAIATDKSYSYILQFAISLGQLYGTFMYYITALLDGDNFSSSP 180
           +EGLT+VLEGPACILA+YAIAT+KSYSYILQFAISLGQLYGTF+YYITA+LDGDNFSS P
Sbjct: 121 IEGLTSVLEGPACILAMYAIATEKSYSYILQFAISLGQLYGTFLYYITAVLDGDNFSSGP 180

Query: 181 IYYYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKV 221
            YYY YYV+ NSFWILIPSLIAIRCWKKIC AM+SE HKK K+
Sbjct: 181 FYYYAYYVVANSFWILIPSLIAIRCWKKICAAMNSEGHKKRKI 223

BLAST of Cp4.1LG03g13440 vs. ExPASy TrEMBL
Match: A0A1S3BY31 (probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Cucumis melo OX=3656 GN=LOC103494647 PE=3 SV=1)

HSP 1 Score: 364 bits (935), Expect = 5.36e-126
Identity = 185/222 (83.33%), Postives = 199/222 (89.64%), Query Frame = 0

Query: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYLLSSLFITLLLWTLSGRLPKKTKI 60
           MAG SEAA HPY PTDL+LP YVPISLPL+TILG YLLSSLF+ LLLWTLSGRL KKTKI
Sbjct: 1   MAGASEAAPHPYVPTDLKLPDYVPISLPLSTILGGYLLSSLFLVLLLWTLSGRLTKKTKI 60

Query: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKD--SSYLAEVWKEYSKGDSRYATRDSAIVS 120
           +RLLMCWWIFTGLTH+VLEGYFVFSPEFYKD  S YLAEVWKEYSKGDSRYA R+S IV 
Sbjct: 61  DRLLMCWWIFTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVG 120

Query: 121 LEGLTAVLEGPACILAVYAIATDKSYSYILQFAISLGQLYGTFMYYITALLDGDNFSSSP 180
           +EGLT+VLEGPACILA YAIA+ KSYSYILQFAISLGQLYGT +YYITA+LDGD+FSS P
Sbjct: 121 IEGLTSVLEGPACILAAYAIASGKSYSYILQFAISLGQLYGTLLYYITAVLDGDDFSSGP 180

Query: 181 IYYYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTK 220
           IYYY YYV+ NSFW+LIPSLIAIRCWKKIC AMS ES  KTK
Sbjct: 181 IYYYSYYVVANSFWLLIPSLIAIRCWKKICNAMSIESQMKTK 222

BLAST of Cp4.1LG03g13440 vs. ExPASy TrEMBL
Match: A0A0A0KVP4 (EXPERA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G007050 PE=3 SV=1)

HSP 1 Score: 362 bits (929), Expect = 4.40e-125
Identity = 182/222 (81.98%), Postives = 198/222 (89.19%), Query Frame = 0

Query: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYLLSSLFITLLLWTLSGRLPKKTKI 60
           MAG SEAA HPY PTDL+LP YVPISLPL+TILG YLL+SLF+ LL+WTLSGRL KK KI
Sbjct: 1   MAGASEAAPHPYVPTDLKLPDYVPISLPLSTILGGYLLTSLFLVLLVWTLSGRLTKKAKI 60

Query: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKD--SSYLAEVWKEYSKGDSRYATRDSAIVS 120
           +RLLMCWWIFTGLTH+VLEGYFVFSPEFYKD  S YLAEVWKEYSKGDSRYA R+S IVS
Sbjct: 61  DRLLMCWWIFTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS 120

Query: 121 LEGLTAVLEGPACILAVYAIATDKSYSYILQFAISLGQLYGTFMYYITALLDGDNFSSSP 180
           +EGLT+VLEGPACILA YAIA+ KSYSYILQFAISLGQLYGT +YY+TA+LDGD+FSS P
Sbjct: 121 IEGLTSVLEGPACILAAYAIASGKSYSYILQFAISLGQLYGTLLYYVTAVLDGDDFSSGP 180

Query: 181 IYYYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTK 220
            YYY YYV+ NSFWILIPSLI IRCWKKICIAMS ES  KTK
Sbjct: 181 FYYYAYYVVANSFWILIPSLIGIRCWKKICIAMSIESQTKTK 222

BLAST of Cp4.1LG03g13440 vs. TAIR 10
Match: AT1G20050.1 (C-8,7 sterol isomerase )

HSP 1 Score: 281.2 bits (718), Expect = 7.3e-76
Identity = 138/224 (61.61%), Postives = 178/224 (79.46%), Query Frame = 0

Query: 6   EAARHPYAPTDLELPGYVPISLPLTTILGVYLLSSLFITLLLWTLSGRLPKKTKIERLLM 65
           E   HPY P DL LPGYVPIS+ +++I+ +YL SSL +  L+W L GR  KK K+++LLM
Sbjct: 2   EELAHPYVPRDLNLPGYVPISMSMSSIVSIYLGSSLLVVSLVWLLFGR--KKAKLDKLLM 61

Query: 66  CWWIFTGLTHIVLEGYFVFSPEFYKD--SSYLAEVWKEYSKGDSRYATRDSAIVSLEGLT 125
           CWW FTGLTH++LEGYFVFSPEF+KD  S+YLAEVWKEYSKGDSRY  RDSA+VS+EG+T
Sbjct: 62  CWWTFTGLTHVILEGYFVFSPEFFKDNTSAYLAEVWKEYSKGDSRYVGRDSAVVSVEGIT 121

Query: 126 AVLEGPACILAVYAIATDKSYSYILQFAISLGQLYGTFMYYITALLDGDNFSSSPIYYYL 185
           AV+ GPA +LA+YAIA +KSYSY+LQ AIS+ QLYG  +Y+ITA+L+GDNF+++  YYY 
Sbjct: 122 AVIVGPASLLAIYAIAKEKSYSYVLQLAISVCQLYGCLVYFITAILEGDNFATNSFYYYS 181

Query: 186 YYVLLNSFWILIPSLIAIRCWKKICIA-------MSSESHKKTK 221
           YY+  N +W+LIPSLI+ RCWKKIC A       + +++ KKT+
Sbjct: 182 YYIGANCWWVLIPSLISFRCWKKICAAAAIANNNVETKTKKKTR 223

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O489621.0e-7461.61Probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Arabidopsis thalia... [more]
Q9FTZ24.3e-6558.77Probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Oryza sativa subsp... [more]
Q9JJ461.8e-3136.993-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Rattus norvegicus OX=10116 ... [more]
P702451.6e-3035.783-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Mus musculus OX=10090 GN=Eb... [more]
Q604908.5e-2937.073-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Cavia porcellus OX=10141 GN... [more]
Match NameE-valueIdentityDescription
XP_023526321.17.97e-155100.00probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Cucurbita pepo subsp... [more]
KAG6581969.13.25e-15499.10putative 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase, partial [Cucurbita a... [more]
XP_022955667.11.32e-15398.65probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Cucurbita moschata][more]
XP_022980063.11.88e-15399.10probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Cucurbita maxima][more]
XP_038881035.13.79e-13385.71probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1GU986.40e-15498.65probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Cucurbita moschata... [more]
A0A6J1IY569.10e-15499.10probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Cucurbita maxima O... [more]
A0A6J1E0U07.96e-12880.72probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Momordica charanti... [more]
A0A1S3BY315.36e-12683.33probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Cucumis melo OX=36... [more]
A0A0A0KVP44.40e-12581.98EXPERA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G007050 PE=3... [more]
Match NameE-valueIdentityDescription
AT1G20050.17.3e-7661.61C-8,7 sterol isomerase [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF05241EBPcoord: 95..203
e-value: 2.9E-21
score: 76.0
NoneNo IPR availablePANTHERPTHR14207:SF7BNAANNG14740D PROTEINcoord: 6..215
IPR007905Emopamil-binding proteinPANTHERPTHR14207STEROL ISOMERASEcoord: 6..215
IPR033118EXPERA domainPROSITEPS51751EXPERAcoord: 60..200
score: 16.28657

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG03g13440.1Cp4.1LG03g13440.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016125 sterol metabolic process
biological_process GO:0016126 sterol biosynthetic process
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0000247 C-8 sterol isomerase activity
molecular_function GO:0047750 cholestenol delta-isomerase activity
molecular_function GO:0004769 steroid delta-isomerase activity