Cp4.1LG03g13260 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG03g13260
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionhydroxyacylglutathione hydrolase 2, mitochondrial-like
LocationCp4.1LG03: 9725748 .. 9730973 (+)
RNA-Seq ExpressionCp4.1LG03g13260
SyntenyCp4.1LG03g13260
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGGTCTCCAAGGCTTCCTCAGCCATGGCCTCCATGCCTTGCACTAGGGTTCGCTTTTACTCTTTGTTCTCTTTCTTTTTCTCTATATTTTCGTTTCGCAATTGCACTCTCTCATGTTTTCTCTGAATTTTTCTTATTGTTTTGTTGGAATGTTTTCGATGTTGTCGGTTTTAAGCTGTGGATACTCTCCAATGTAGCTGAAGCAGTCTCGCGGTTTATTTTGTTTTGAATCTTTGTTGATCGGCCTTGTTATATCTTCTGCTACTTTCTCTTACAGCATGACGATGAATATCTAACGGATTCGATTTTGTTGCTCCTTTTGTTTGAAATTGGTACTGCTAATTTTGTTGCTAAGATCTGTAACATCATGATGTATGATCCTTCCTTCTCGTGTCTTGTATATCTTGTCATAGTGGGCAGTAAGTCTGTAATGTTGTCGGGGTTTATTGATAGATTTATTGAACAGAAACATGTGTTTTAGCTTTATTGACGAATGGGAGTTTCAGGTTGGAAGCGTATGTGTCTGGCCGAGCATGAAACAGCTTTCTTTTAGAAAAGACCTTTTGTATGGGGTTATGAGACTCTTATCTATACCATTGAAAACATTGCGTGGAGCTAGTCGTTCACTTAAAGTTGCTCAGTTCTGCAGTGTCGTCAATATGTCTTCCACATTGCAAATTGAACTGGTAAGCTGTTAGATCTGGAAATTGCTTTTATGGTGTCAATGGCATCAGTTCTTTTCTCCCATGATGGTTTTGTGGTGGTCATTTTATGATTACTTTCTTTGGTTAACATTTTCTTTCTAAATTCATGCTTTAATTCTAAAAAAATTTCATCTGAGAGAATTTCTAATTGTAGGAAGAGGCCCGAATGGTCTAAATTTCAGTGTGTTATTTCTTTCCAAAGTTAGATTGCTTCAACTATTGGCATCCATAATATATAACTTTCACCTGCTCAGCTACACGATATATAATTCTTGATCTGAGTCCATTCAAGTAAGGTGGCTACAAATTATTTTTAATAAGACATTAGCCATGCTCTTTTTGTGCTCTCACCCTAAATGAATCTATTTTTAGGATTGCTGGTTGTCCACTATCATTGTACGTTCTGCATCCCATAATAACTGCTTAGTCAGCTGCAGCATAAACATTCATTGCGTATCTCTATTTTATTCTACTCATTCTTCGATAGGTACCTTGTCTTCGTGACAACTATGCATACCTCTTACATGATGTTGATACGGGCACAGTTGGGGTTGTTGATCCTTCTGAAGCTGTACCAGTTATAGATGCTTTAAGAAAGAAAAACAGAAATTTGACATATATACTTAATACTCATCATCATCATGATCACACTGGTGGGAATGAAGAATTAAAAGCAAGGTACGGAGCAAAGGTATGAATTTAATTTTTTTTTTGCATTGCATCAATATTCTAGTTGGTCTCATCCTATAAATGTTGTATGTTTGAGTCCTAAAGGTTGGTCATAGAGGTAAGAACCTGTGTACCTAACCTAGCACAAGACTTCTTGAGGCCATTATGTGAGAAATTCATTGACTCTCAATTTCTCGATGCCAGAAGAATGTTTATATATATTAGTCTCTATTCTTAATCCTTTTATCTAGTAAACATAGTGGTGAATATTTTTTTAAATATAAACACAGATATCTATTGTCATGAGAACATCATACCTTATCACGTGCAAGCAATGTTACATTCTTCAGTACTGATCAGAAAGTATTTTGTGTATTTTGACTTGTTTTGAATTAAAGACTTCCCTAACTTGTCCTTTAAATTAAGGCTTGGATTGAAATGAATTAGGGAATTGATGCTGACTTATGTTATACAATAGTCCATGAGATATTTCTTATCCTTGAATTGAGGGTTTACTTTTACAATCCATAGAACTTCTGTCAATTTTTTATACCCTTTTTTCTGGTTGTAGGTGATTGGCTCTGGAATAGATAGCGACAGAATTCCTGGAATTGACATAGCTTTAAAAGATGGAGACAAGTGGATGTTTGCTGGGCATGAAGTGCAAGTAATGGAAACTCCTGGTCATACTCGAGGTGTTTGTTACTTGTTACTTTAGATTAGTTTCTTGTATGATAAGATGTTGTAGACCACGGGATTAAAGGAGATCATTCTCCAAGAGATCTAATCCCAATCTTTATTATGATTCAAAATGTGTAGAATATATGATAGAAACCCTTACTTATAGGGAATTAGAGAGCAAACTAAAGTCCAATCCAAAAATACAGTTAGATAGTTAAAAAAGGATTAGTCAAGGAATTGTAGTAATTATCTAAGATTAATCTAATCCTACAATCCTATTAAATCAAAATAGGTCCGCTAGATTTGTTTCTTGTATGATAACCCTCCCCTTCCTCCGGACGTGCATGAAAAATGAGGAAATAAAAGAAGAGAGTGAGAATAGAAATGGAAACTTCTATTGTTATTCTCTATCATTGCTACATTGTCTCTCATATGGTTTAGCCTTGCTTCTCTCTCCCTATTGGATTTAACTTTGATTATTATTCTTTATTAATTTTCTCATAGCTGATTTTTCCTTGGATACTTCATGTGGGTTTGTTGTGCCTATGGAACTCGGGCTTCTCACCATTCTATACTTTTAGGCCCTGATTTGTTGTTTTTGATTTTGACTGAATTCAGGTCATATTAGCTTCTACTTTCCTGGATCCGCTGCAATATTTACTGGAGATACTTTATTCAGCTTATCTTGTGGCAAGCTTTTTGAAGGAACTCCTGAACAGGTTTGTTTGTCTTATATTTTTCATTTAATTGGATGTTATCTAAATGTGGTACTTCAAGAATGCGGAATGCCAGATAATTTTTTCTTGTTAGATGTACTTATGAATTATTATCTGCTTCTTTGTGCTTTATTTTTCGCTCCTTTGGCTCTTGTCCTGTTGTCCCACTGAGTTTAAGAATATTTGACTAAAGGATTACTCAAAATCTTGTATGTTGGATTGGAAGTCATCTATTGTTAACCTTAATGATATTCAGTCTGTTGTTGAAGTCATTTCATGGCTTAAGATTGATGGAGATGAGAGCTCTTTGATTGGTATGGTTTGTCAGGCCTCCAATCTTGAGAGATTAGTGTCTTTAGGGGTGAGGTTTTGAGTGGGATTCCATATTTTTCCTTCATAATTCAAGATTCCTTCCTTTGTTAGTTTACGGAAGAAAAACTTCAGGAAGGACTTCTTCTCGGAAGGGGTCCAGGGTGTCTCGTTTAGTCAGATGGAAGATTGTGTTGTGTTCAATCAAACTAGGTGGATTTGTATAGGGAATTTGAAACTTTGTGATGAAGCCTTGCGAAGTCGTTTCTTTTGATGCTAATGCTATATTAACATAGGGTTATAGTGCGCAAATATGACCCTCATTTAGCTACGTGTGCTTCAATTTGTGGGTGTAAAGATACTTTTAGAATTGTATGAACATCTTATCTCATCTGGTTATCTAACGTTCTCTAAGTTTTTGAAGTTCTCTATTCAAAATTGAACAGCAGGGTGAAGGATAACACCCCATCCTTAGCTATTTAAGCCCTATCTTTGTCACTTCTCTCAGAAATTTGTTTCAGTGGCTTTTATCCTTTTTAAGGTTTCTATTTTCTACAACTTCGGTTTTGGCCTTCCCTGGTCTGATAGGTGGATCTTTTAAGATTTCCTCATTGCTCTCCTTGTTCTCCTGGCCTTGCCTTGGGCTAGGGATGTTCCTCAACGTGCTGCCATTTCTTTGGGGGAGATTTTCTGTTGTTCCATTTATTCTCTTCCCACGTACCCTGTTTTGTGGTTCTCTCATGAATAAAATGGCACTCAGCTTCAATATGCTTTGTTCACTCATGAAGTATCTATATCCATGTTATGGAAAAACTATTGTTATTAAAGAGTGAATTTGCTGCTGAATGTGAATTAAGCTTAGAAACTTGATTGCTTTTAGTTATTCAGATTCTAATTGTTTATATCACAGATGCTATCTTCCCTTAAAAAGATCACTTCTTTGCCAGATGATACCAACATATTTTGCGGTCATGAATATACTCTGGTCAGTAACTTATTGACCTTCCTTTATTTATTTTCTACGATACTCCAATTTTTTAAGCTTGAATGCCATTTGCCTAATTGTAAGGTTACCATTTAGCAGAGTAACTCTAAGTTCGCTATGTCCATTGAACCTAAGAACGAGGCTCTCAAATCTTATGCATCCCAGGTTGCCAATCTACGAAGCAAGGGCTTGCCAACGGTAACTTTTTAGCTATTCTTGTATGGATGGTTATTTGATATTTAAATTGGTTACTAGACAAATTTCTTTCAATTTGCGTAAATATGGGTTATTGTTTCTTGCATTTGAAAACCATTGCCTATTTGAGTATTTTGAATTTCCTTTCATTTGAATTGAAGATCAAAAGAACTATCACTTCCTGTTTGTATGGTAGAATTGTTTTAAACTTTACATATAATCGTAGAGGTATCGTGTGTGGATAGAAGCTGCATCGTAGCAGTATGTGGCTAAACCTATTGTCTCTTAAAAAACCTTTTTTGGATAAGGAACTATTTGATTAAAAGCATGAACTTTTTTGTATTCTTCAGTCGTGAGTTTGCTTGTCCTGTATTTTATGGAGAGTTATTTGTGATGCTATGAGCTCCTTGGAAAATTTGACTTTGTTCTATGAAAACAATAATTTTGATATGCCATGAACATGGCATTCATGATATATCCTTCCATCACCCCAAGTATTTTTGAACTTTGATTTTAATATCTCAGATTCCAGCAACATTAAAGATGGAGAAAATGTGCAATCCATTCCTCCGTACATCAAGTATCGAAATTCGGCAGTCGTTAAACGTTCAAAATACAGCGGATGATGCAGAGGTGTTGGGTATCATTCGCAGAGCCAAGGATAATTTTTAGTCACCCCCTACAAAACCTCAAAAGAAGAGGGGGGTGGGAAAGTTCATTTTGGTGTATAGGATGATGATAGGTTAGCCATATGCATGTGTAAAATAAGTTAACCGAGATCTTTGTATCGATGATTTCCTACAAGTTCCATTATCAGTTCAGTTCTTAATTTTAAAATTTGCACCTCTCCTGCCACGAGAATTCAATCTTTTTTCTTTTCCCTCTTTTGAAATGAAGAGTGCAATCATTTGATCCCTGATGTATATTCATTATTACTATTGTTATATAATTATTTGATTTCATT

mRNA sequence

ATGATGGTTGGAAGCGTATGTGTCTGGCCGAGCATGAAACAGCTTTCTTTTAGAAAAGACCTTTTGTATGGGGTTATGAGACTCTTATCTATACCATTGAAAACATTGCGTGGAGCTAGTCGTTCACTTAAAGTTGCTCAGTTCTGCAGTGTCGTCAATATGTCTTCCACATTGCAAATTGAACTGGTGATTGGCTCTGGAATAGATAGCGACAGAATTCCTGGAATTGACATAGCTTTAAAAGATGGAGACAAGTGGATGTTTGCTGGGCATGAAGTGCAAGTAATGGAAACTCCTGGTCATACTCGAGGTCATATTAGCTTCTACTTTCCTGGATCCGCTGCAATATTTACTGGAGATACTTTATTCAGCTTATCTTGTGGCAAGCTTTTTGAAGGAACTCCTGAACAGATGCTATCTTCCCTTAAAAAGATCACTTCTTTGCCAGATGATACCAACATATTTTGCGGTCATGAATATACTCTGAGTAACTCTAAGTTCGCTATGTCCATTGAACCTAAGAACGAGGCTCTCAAATCTTATGCATCCCAGGTTGCCAATCTACGAAGCAAGGGCTTGCCAACGATTCCAGCAACATTAAAGATGGAGAAAATGTGCAATCCATTCCTCCGTACATCAAGTATCGAAATTCGGCAGTCGTTAAACGTTCAAAATACAGCGGATGATGCAGAGGTGTTGGGTATCATTCGCAGAGCCAAGGATAATTTTTAGTCACCCCCTACAAAACCTCAAAAGAAGAGGGGGGTGGGAAAGTTCATTTTGGTGTATAGGATGATGATAGGTTAGCCATATGCATGTGTAAAATAAGTTAACCGAGATCTTTGTATCGATGATTTCCTACAAGTTCCATTATCAGTTCAGTTCTTAATTTTAAAATTTGCACCTCTCCTGCCACGAGAATTCAATCTTTTTTCTTTTCCCTCTTTTGAAATGAAGAGTGCAATCATTTGATCCCTGATGTATATTCATTATTACTATTGTTATATAATTATTTGATTTCATT

Coding sequence (CDS)

ATGATGGTTGGAAGCGTATGTGTCTGGCCGAGCATGAAACAGCTTTCTTTTAGAAAAGACCTTTTGTATGGGGTTATGAGACTCTTATCTATACCATTGAAAACATTGCGTGGAGCTAGTCGTTCACTTAAAGTTGCTCAGTTCTGCAGTGTCGTCAATATGTCTTCCACATTGCAAATTGAACTGGTGATTGGCTCTGGAATAGATAGCGACAGAATTCCTGGAATTGACATAGCTTTAAAAGATGGAGACAAGTGGATGTTTGCTGGGCATGAAGTGCAAGTAATGGAAACTCCTGGTCATACTCGAGGTCATATTAGCTTCTACTTTCCTGGATCCGCTGCAATATTTACTGGAGATACTTTATTCAGCTTATCTTGTGGCAAGCTTTTTGAAGGAACTCCTGAACAGATGCTATCTTCCCTTAAAAAGATCACTTCTTTGCCAGATGATACCAACATATTTTGCGGTCATGAATATACTCTGAGTAACTCTAAGTTCGCTATGTCCATTGAACCTAAGAACGAGGCTCTCAAATCTTATGCATCCCAGGTTGCCAATCTACGAAGCAAGGGCTTGCCAACGATTCCAGCAACATTAAAGATGGAGAAAATGTGCAATCCATTCCTCCGTACATCAAGTATCGAAATTCGGCAGTCGTTAAACGTTCAAAATACAGCGGATGATGCAGAGGTGTTGGGTATCATTCGCAGAGCCAAGGATAATTTTTAG

Protein sequence

MMVGSVCVWPSMKQLSFRKDLLYGVMRLLSIPLKTLRGASRSLKVAQFCSVVNMSSTLQIELVIGSGIDSDRIPGIDIALKDGDKWMFAGHEVQVMETPGHTRGHISFYFPGSAAIFTGDTLFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFAMSIEPKNEALKSYASQVANLRSKGLPTIPATLKMEKMCNPFLRTSSIEIRQSLNVQNTADDAEVLGIIRRAKDNF
Homology
BLAST of Cp4.1LG03g13260 vs. ExPASy Swiss-Prot
Match: Q8LDW8 (Probable hydroxyacylglutathione hydrolase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=GLX2-4 PE=2 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 1.5e-90
Identity = 173/310 (55.81%), Postives = 207/310 (66.77%), Query Frame = 0

Query: 2   MVGSVCVWPSMKQLSFRKDLLYGVMRLLSIPLKTLRGASRSLKVAQFCSVVNMSSTLQIE 61
           +V   CV P ++QL  RK L+ GVM+L S PL+TLR A +S+++++FCSV N+SS+LQIE
Sbjct: 20  LVSQPCVRPCVRQLHVRKGLVSGVMKLFSSPLRTLRDAGKSVRISRFCSVSNVSSSLQIE 79

Query: 62  L----------------------------------------------------------- 121
           L                                                           
Sbjct: 80  LVPCLTDNYAYILHDEDTGTVGVVDPSEAVPVMDALQKNSRNLTYILNTHHHYDHTGGNL 139

Query: 122 ---------VIGSGIDSDRIPGIDIALKDGDKWMFAGHEVQVMETPGHTRGHISFYFPGS 181
                    VIGS  D DRIPGID+ALKD DKWMFAGHEV +METPGHTRGHISFYFPG+
Sbjct: 140 ELKDRYGAKVIGSAADRDRIPGIDVALKDADKWMFAGHEVHIMETPGHTRGHISFYFPGA 199

Query: 182 AAIFTGDTLFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFAMSIEP 241
            AIFTGDTLFSLSCGKLFEGTPEQML+SL++I +LPDDT+++CGHEYTLSNSKFA+SIEP
Sbjct: 200 RAIFTGDTLFSLSCGKLFEGTPEQMLASLQRIIALPDDTSVYCGHEYTLSNSKFALSIEP 259

Query: 242 KNEALKSYASQVANLRSKGLPTIPATLKMEKMCNPFLRTSSIEIRQSLNVQNTADDAEVL 244
            NE L+SYA+ VA LR K LPTIP T+KMEK CNPFLRT + +IR++L +  TAD+AE L
Sbjct: 260 TNEVLQSYAAYVAELRDKKLPTIPTTMKMEKACNPFLRTENTDIRRALGIPETADEAEAL 319

BLAST of Cp4.1LG03g13260 vs. ExPASy Swiss-Prot
Match: Q9SID3 (Hydroxyacylglutathione hydrolase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At2g31350 PE=1 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 1.2e-87
Identity = 170/302 (56.29%), Postives = 207/302 (68.54%), Query Frame = 0

Query: 10  PSMKQLSFRKDLLYGVMRLLSIPLKTLRGASRSLKVAQFCSVVNMSSTLQIEL------- 69
           P ++QL+ RK L+  VM+L+S PL+TLRGA +S++V++FCSV N+SS LQIEL       
Sbjct: 24  PCVRQLNIRKSLVCRVMKLVSSPLRTLRGAGKSIRVSKFCSVSNVSS-LQIELVPCLKDN 83

Query: 70  ------------------------------------------------------------ 129
                                                                       
Sbjct: 84  YAYILHDEDTGTVGVVDPSEAEPIIDSLKRSGRNLTYILNTHHHYDHTGGNLELKDRYGA 143

Query: 130 -VIGSGIDSDRIPGIDIALKDGDKWMFAGHEVQVMETPGHTRGHISFYFPGSAAIFTGDT 189
            VIGS +D DRIPGID+ALKDGDKWMFAGHEV VM+TPGHT+GHIS YFPGS AIFTGDT
Sbjct: 144 KVIGSAMDKDRIPGIDMALKDGDKWMFAGHEVHVMDTPGHTKGHISLYFPGSRAIFTGDT 203

Query: 190 LFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFAMSIEPKNEALKSY 244
           +FSLSCGKLFEGTP+QML+SL+KITSLPDDT+I+CGHEYTLSNSKFA+S+EP NE L+SY
Sbjct: 204 MFSLSCGKLFEGTPKQMLASLQKITSLPDDTSIYCGHEYTLSNSKFALSLEPNNEVLQSY 263

BLAST of Cp4.1LG03g13260 vs. ExPASy Swiss-Prot
Match: O24495 (Hydroxyacylglutathione hydrolase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=GLX2-1 PE=2 SV=2)

HSP 1 Score: 321.2 bits (822), Expect = 9.8e-87
Identity = 165/308 (53.57%), Postives = 204/308 (66.23%), Query Frame = 0

Query: 4   GSVCVWPSMKQLSFRKDLLYGVMRLLSIPLKTLRGASRSLKVAQFCSVVNMSSTLQIEL- 63
           G +CVWP ++QL  RK LLYGVM LLS+PLKTLRGA ++LK+  FCS+ NM S+L+IEL 
Sbjct: 24  GQLCVWPGLRQLCLRKSLLYGVMWLLSMPLKTLRGARKTLKITHFCSISNMPSSLKIELV 83

Query: 64  ------------------------------------------------------------ 123
                                                                       
Sbjct: 84  PCSKDNYAYLLHDEDTGTVGVVDPSEAAPVIEALSRKNWNLTYILNTHHHDDHIGGNAEL 143

Query: 124 -------VIGSGIDSDRIPGIDIALKDGDKWMFAGHEVQVMETPGHTRGHISFYFPGSAA 183
                  VIGS +D DRIPGIDI LKD DKWMFAGHEV++++TPGHT+GHISFYFPGSA 
Sbjct: 144 KERYGAKVIGSAVDKDRIPGIDILLKDSDKWMFAGHEVRILDTPGHTQGHISFYFPGSAT 203

Query: 184 IFTGDTLFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFAMSIEPKN 243
           IFTGD ++SLSCG L EGTPEQMLSSL+KI SLPDDTNI+CG E T  N KFA+S+EPKN
Sbjct: 204 IFTGDLIYSLSCGTLSEGTPEQMLSSLQKIVSLPDDTNIYCGRENTAGNLKFALSVEPKN 263

BLAST of Cp4.1LG03g13260 vs. ExPASy Swiss-Prot
Match: A6WXE0 (Hydroxyacylglutathione hydrolase OS=Ochrobactrum anthropi (strain ATCC 49188 / DSM 6882 / JCM 21032 / NBRC 15819 / NCTC 12168) OX=439375 GN=gloB PE=3 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 2.9e-46
Identity = 88/181 (48.62%), Postives = 125/181 (69.06%), Query Frame = 0

Query: 63  VIGSGIDSDRIPGIDIALKDGDKWMFAGHEVQVMETPGHTRGHISFYFPGSAAIFTGDTL 122
           +IG   +  +IPGID  +KDGD++ F    V+V+ TPGHT G +S+Y P + A+FTGDTL
Sbjct: 81  IIGPKAEETKIPGIDRTVKDGDEFTFGLFRVKVIATPGHTAGEVSYYLPDAKAVFTGDTL 140

Query: 123 FSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFAMSIEPKNEALKSYA 182
           F+L CG+LFEGTP  M  SL+K+ +LP DT ++CGHEYT SN++FA++I+P N ALK  A
Sbjct: 141 FALGCGRLFEGTPLTMFQSLQKLVALPGDTAVYCGHEYTESNARFALTIDPANSALKERA 200

Query: 183 SQVANLRSKGLPTIPATLKMEKMCNPFLRTSSIEIRQSLNVQNTADDAEVLGIIRRAKDN 242
           +++A LR+    T+P+++ +E   NPFLR     IR  L +Q+  D+A V   IR+ KD 
Sbjct: 201 AEIARLRAADRMTLPSSIALEMATNPFLRWHDAGIRSRLGLQDAPDEA-VFAEIRKRKDM 260

Query: 243 F 244
           F
Sbjct: 261 F 260

BLAST of Cp4.1LG03g13260 vs. ExPASy Swiss-Prot
Match: Q5LNN5 (Hydroxyacylglutathione hydrolase OS=Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) OX=246200 GN=gloB PE=3 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 2.4e-45
Identity = 86/181 (47.51%), Postives = 127/181 (70.17%), Query Frame = 0

Query: 63  VIGSGIDSDRIPGIDIALKDGDKWMFAGHEVQVMETPGHTRGHISFYFPGSAAIFTGDTL 122
           VIG+  D+ R+P +D+A+ +GD++   G  V+V++  GHT GHI+F+ P   A+FT D+L
Sbjct: 76  VIGAEADAHRLPPLDLAVSEGDRFDLGGAPVEVLDVSGHTVGHIAFHLPTGKAVFTADSL 135

Query: 123 FSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFAMSIEPKNEALKSYA 182
            +L CG+LFEGTP+QM +SL K+ +LPDDT +  GHEYT SN++FA+S++P N ALK+ A
Sbjct: 136 MALGCGRLFEGTPDQMWASLSKLAALPDDTLVCSGHEYTQSNARFALSVDPDNAALKTRA 195

Query: 183 SQVANLRSKGLPTIPATLKMEKMCNPFLRTSSIEIRQSLNVQNTADDAEVLGIIRRAKDN 242
           +++   R++G PT+P+ L +EK  NPFLR +   I+  L +Q  AD A V   IR  KD+
Sbjct: 196 AEIDQARAEGRPTVPSLLSLEKSTNPFLRAADPGIQSLLGMQG-ADPARVFAEIRARKDH 255

Query: 243 F 244
           F
Sbjct: 256 F 255

BLAST of Cp4.1LG03g13260 vs. NCBI nr
Match: XP_023528264.1 (probable hydroxyacylglutathione hydrolase 2, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo] >XP_023528265.1 probable hydroxyacylglutathione hydrolase 2, chloroplastic isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 448 bits (1153), Expect = 6.50e-157
Identity = 241/309 (77.99%), Postives = 241/309 (77.99%), Query Frame = 0

Query: 3   VGSVCVWPSMKQLSFRKDLLYGVMRLLSIPLKTLRGASRSLKVAQFCSVVNMSSTLQIEL 62
           VGSVCVWPSMKQLSFRKDLLYGVMRLLSIPLKTLRGASRSLKVAQFCSVVNMSSTLQIEL
Sbjct: 20  VGSVCVWPSMKQLSFRKDLLYGVMRLLSIPLKTLRGASRSLKVAQFCSVVNMSSTLQIEL 79

Query: 63  V----------------------------------------------------------- 122
           V                                                           
Sbjct: 80  VPCLRDNYAYLLHDVDTGTVGVVDPSEAVPVIDALRKKNRNLTYILNTHHHHDHTGGNEE 139

Query: 123 ---------IGSGIDSDRIPGIDIALKDGDKWMFAGHEVQVMETPGHTRGHISFYFPGSA 182
                    IGSGIDSDRIPGIDIALKDGDKWMFAGHEVQVMETPGHTRGHISFYFPGSA
Sbjct: 140 LKARYGAKVIGSGIDSDRIPGIDIALKDGDKWMFAGHEVQVMETPGHTRGHISFYFPGSA 199

Query: 183 AIFTGDTLFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFAMSIEPK 242
           AIFTGDTLFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFAMSIEPK
Sbjct: 200 AIFTGDTLFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFAMSIEPK 259

BLAST of Cp4.1LG03g13260 vs. NCBI nr
Match: XP_022980059.1 (probable hydroxyacylglutathione hydrolase 2, chloroplastic [Cucurbita maxima])

HSP 1 Score: 447 bits (1150), Expect = 1.86e-156
Identity = 240/309 (77.67%), Postives = 241/309 (77.99%), Query Frame = 0

Query: 3   VGSVCVWPSMKQLSFRKDLLYGVMRLLSIPLKTLRGASRSLKVAQFCSVVNMSSTLQIEL 62
           VGSVCVWPSMKQLSFRKDLLYGVMRLLSIPLKTLRGASRSLKVAQFCSVVNMSSTLQIEL
Sbjct: 20  VGSVCVWPSMKQLSFRKDLLYGVMRLLSIPLKTLRGASRSLKVAQFCSVVNMSSTLQIEL 79

Query: 63  V----------------------------------------------------------- 122
           V                                                           
Sbjct: 80  VPCLRDNYAYLLHDVDTGTVGVVDPSEAVPVIDALRKKNRNLTYILNTHHHHDHTGGNEE 139

Query: 123 ---------IGSGIDSDRIPGIDIALKDGDKWMFAGHEVQVMETPGHTRGHISFYFPGSA 182
                    IGSGIDSDRIPGIDIALKDGDKWMFAGHEVQVMETPGHTRGHISFYFPGSA
Sbjct: 140 LKARYGAKVIGSGIDSDRIPGIDIALKDGDKWMFAGHEVQVMETPGHTRGHISFYFPGSA 199

Query: 183 AIFTGDTLFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFAMSIEPK 242
           AIFTGDTLFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFAMSIEPK
Sbjct: 200 AIFTGDTLFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFAMSIEPK 259

BLAST of Cp4.1LG03g13260 vs. NCBI nr
Match: XP_022956058.1 (probable hydroxyacylglutathione hydrolase 2, chloroplastic [Cucurbita moschata])

HSP 1 Score: 446 bits (1147), Expect = 5.32e-156
Identity = 239/309 (77.35%), Postives = 241/309 (77.99%), Query Frame = 0

Query: 3   VGSVCVWPSMKQLSFRKDLLYGVMRLLSIPLKTLRGASRSLKVAQFCSVVNMSSTLQIEL 62
           VGSVCVWPSMKQLSFRKDLLYGVMRLLSIPLKTLRGASRSLKVAQFCSVVNMSSTLQIEL
Sbjct: 20  VGSVCVWPSMKQLSFRKDLLYGVMRLLSIPLKTLRGASRSLKVAQFCSVVNMSSTLQIEL 79

Query: 63  V----------------------------------------------------------- 122
           V                                                           
Sbjct: 80  VPCLRDNYAYLLHDVDTGTVGVVDPSEAVPVIDALRKKNRNLTYILNTHHHHDHTGGNEE 139

Query: 123 ---------IGSGIDSDRIPGIDIALKDGDKWMFAGHEVQVMETPGHTRGHISFYFPGSA 182
                    IGSGIDSDRIPGIDIALKDGDKWMFAGHEVQVMETPGHTRGHISFYFPGSA
Sbjct: 140 LKARYGAKVIGSGIDSDRIPGIDIALKDGDKWMFAGHEVQVMETPGHTRGHISFYFPGSA 199

Query: 183 AIFTGDTLFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFAMSIEPK 242
           AIFTGDTLFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFAMSIEPK
Sbjct: 200 AIFTGDTLFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFAMSIEPK 259

BLAST of Cp4.1LG03g13260 vs. NCBI nr
Match: KAG6581991.1 (putative hydroxyacylglutathione hydrolase 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 445 bits (1145), Expect = 1.07e-155
Identity = 238/309 (77.02%), Postives = 241/309 (77.99%), Query Frame = 0

Query: 3   VGSVCVWPSMKQLSFRKDLLYGVMRLLSIPLKTLRGASRSLKVAQFCSVVNMSSTLQIEL 62
           VGSVCVWPSMKQLSFRKDLLYGVMRLLSIPLKTLRGASRSLKVAQFCSVVNMSSTLQIEL
Sbjct: 20  VGSVCVWPSMKQLSFRKDLLYGVMRLLSIPLKTLRGASRSLKVAQFCSVVNMSSTLQIEL 79

Query: 63  V----------------------------------------------------------- 122
           V                                                           
Sbjct: 80  VPCLRDNYAYLLHDVDTGTVGVVDPSEAVPVIDALRKKNRNLTYILNTHHHHDHTGGNEE 139

Query: 123 ---------IGSGIDSDRIPGIDIALKDGDKWMFAGHEVQVMETPGHTRGHISFYFPGSA 182
                    IGSGIDSDRIPGIDIALKDGDKWMFAGHEVQVMETPGHTRGH+SFYFPGSA
Sbjct: 140 LKARYGAKVIGSGIDSDRIPGIDIALKDGDKWMFAGHEVQVMETPGHTRGHLSFYFPGSA 199

Query: 183 AIFTGDTLFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFAMSIEPK 242
           AIFTGDTLFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFAMSIEPK
Sbjct: 200 AIFTGDTLFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFAMSIEPK 259

BLAST of Cp4.1LG03g13260 vs. NCBI nr
Match: KAG7018412.1 (putative hydroxyacylglutathione hydrolase 2, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 446 bits (1148), Expect = 5.98e-155
Identity = 239/310 (77.10%), Postives = 242/310 (78.06%), Query Frame = 0

Query: 2   MVGSVCVWPSMKQLSFRKDLLYGVMRLLSIPLKTLRGASRSLKVAQFCSVVNMSSTLQIE 61
           +VGSVCVWPSMKQLSFRKDLLYGVMRLLSIPLKTLRGASRSLKVAQFCSVVNMSSTLQIE
Sbjct: 98  LVGSVCVWPSMKQLSFRKDLLYGVMRLLSIPLKTLRGASRSLKVAQFCSVVNMSSTLQIE 157

Query: 62  LV---------------------------------------------------------- 121
           LV                                                          
Sbjct: 158 LVPCLRDNYAYLLHDVDTGTVGVVDPSEAVPVIDALRKKNRNLTYILNTHHHHDHTGGNE 217

Query: 122 ----------IGSGIDSDRIPGIDIALKDGDKWMFAGHEVQVMETPGHTRGHISFYFPGS 181
                     IGSGIDSDRIPGIDIALKDGDKWMFAGHEVQVMETPGHTRGHISFYFPGS
Sbjct: 218 ELKARYGAKVIGSGIDSDRIPGIDIALKDGDKWMFAGHEVQVMETPGHTRGHISFYFPGS 277

Query: 182 AAIFTGDTLFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFAMSIEP 241
           AAIFTGDTLFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFAMSIEP
Sbjct: 278 AAIFTGDTLFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFAMSIEP 337

Query: 242 KNEALKSYASQVANLRSKGLPTIPATLKMEKMCNPFLRTSSIEIRQSLNVQNTADDAEVL 243
           KNEALKSYA+QVANLRSKGLPTIPATLKMEKMCNPFLRTSSIEIRQSLNVQNTADDAEVL
Sbjct: 338 KNEALKSYATQVANLRSKGLPTIPATLKMEKMCNPFLRTSSIEIRQSLNVQNTADDAEVL 397

BLAST of Cp4.1LG03g13260 vs. ExPASy TrEMBL
Match: A0A6J1IYA2 (probable hydroxyacylglutathione hydrolase 2, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111479575 PE=3 SV=1)

HSP 1 Score: 447 bits (1150), Expect = 9.00e-157
Identity = 240/309 (77.67%), Postives = 241/309 (77.99%), Query Frame = 0

Query: 3   VGSVCVWPSMKQLSFRKDLLYGVMRLLSIPLKTLRGASRSLKVAQFCSVVNMSSTLQIEL 62
           VGSVCVWPSMKQLSFRKDLLYGVMRLLSIPLKTLRGASRSLKVAQFCSVVNMSSTLQIEL
Sbjct: 20  VGSVCVWPSMKQLSFRKDLLYGVMRLLSIPLKTLRGASRSLKVAQFCSVVNMSSTLQIEL 79

Query: 63  V----------------------------------------------------------- 122
           V                                                           
Sbjct: 80  VPCLRDNYAYLLHDVDTGTVGVVDPSEAVPVIDALRKKNRNLTYILNTHHHHDHTGGNEE 139

Query: 123 ---------IGSGIDSDRIPGIDIALKDGDKWMFAGHEVQVMETPGHTRGHISFYFPGSA 182
                    IGSGIDSDRIPGIDIALKDGDKWMFAGHEVQVMETPGHTRGHISFYFPGSA
Sbjct: 140 LKARYGAKVIGSGIDSDRIPGIDIALKDGDKWMFAGHEVQVMETPGHTRGHISFYFPGSA 199

Query: 183 AIFTGDTLFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFAMSIEPK 242
           AIFTGDTLFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFAMSIEPK
Sbjct: 200 AIFTGDTLFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFAMSIEPK 259

BLAST of Cp4.1LG03g13260 vs. ExPASy TrEMBL
Match: A0A6J1GVR0 (probable hydroxyacylglutathione hydrolase 2, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111457866 PE=3 SV=1)

HSP 1 Score: 446 bits (1147), Expect = 2.58e-156
Identity = 239/309 (77.35%), Postives = 241/309 (77.99%), Query Frame = 0

Query: 3   VGSVCVWPSMKQLSFRKDLLYGVMRLLSIPLKTLRGASRSLKVAQFCSVVNMSSTLQIEL 62
           VGSVCVWPSMKQLSFRKDLLYGVMRLLSIPLKTLRGASRSLKVAQFCSVVNMSSTLQIEL
Sbjct: 20  VGSVCVWPSMKQLSFRKDLLYGVMRLLSIPLKTLRGASRSLKVAQFCSVVNMSSTLQIEL 79

Query: 63  V----------------------------------------------------------- 122
           V                                                           
Sbjct: 80  VPCLRDNYAYLLHDVDTGTVGVVDPSEAVPVIDALRKKNRNLTYILNTHHHHDHTGGNEE 139

Query: 123 ---------IGSGIDSDRIPGIDIALKDGDKWMFAGHEVQVMETPGHTRGHISFYFPGSA 182
                    IGSGIDSDRIPGIDIALKDGDKWMFAGHEVQVMETPGHTRGHISFYFPGSA
Sbjct: 140 LKARYGAKVIGSGIDSDRIPGIDIALKDGDKWMFAGHEVQVMETPGHTRGHISFYFPGSA 199

Query: 183 AIFTGDTLFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFAMSIEPK 242
           AIFTGDTLFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFAMSIEPK
Sbjct: 200 AIFTGDTLFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFAMSIEPK 259

BLAST of Cp4.1LG03g13260 vs. ExPASy TrEMBL
Match: A0A1S3BZ30 (probable hydroxyacylglutathione hydrolase 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103494619 PE=3 SV=1)

HSP 1 Score: 426 bits (1096), Expect = 1.50e-148
Identity = 228/309 (73.79%), Postives = 235/309 (76.05%), Query Frame = 0

Query: 3   VGSVCVWPSMKQLSFRKDLLYGVMRLLSIPLKTLRGASRSLKVAQFCSVVNMSSTLQIEL 62
           V SVCVWPS+KQLSFRKDLLYGVMRLLS PLKTLRGASRSLKVAQFCSVVNMSSTLQIEL
Sbjct: 20  VRSVCVWPSVKQLSFRKDLLYGVMRLLSTPLKTLRGASRSLKVAQFCSVVNMSSTLQIEL 79

Query: 63  V----------------------------------------------------------- 122
           V                                                           
Sbjct: 80  VPCLRDNYAYLLHDVDTGTVGVVDPSEALPVIDALSKKNRNLTYILNTHHHHDHTGGNEE 139

Query: 123 ---------IGSGIDSDRIPGIDIALKDGDKWMFAGHEVQVMETPGHTRGHISFYFPGSA 182
                    IGSGIDSDRIPGIDI+LKDGDKWMFAGHEV VMETPGHTRGHISFYFPGSA
Sbjct: 140 LKARYGAKVIGSGIDSDRIPGIDISLKDGDKWMFAGHEVHVMETPGHTRGHISFYFPGSA 199

Query: 183 AIFTGDTLFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFAMSIEPK 242
           AIFTGDTLFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFA+SIEPK
Sbjct: 200 AIFTGDTLFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFALSIEPK 259

BLAST of Cp4.1LG03g13260 vs. ExPASy TrEMBL
Match: A0A0A0KYR2 (Lactamase_B domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G009870 PE=3 SV=1)

HSP 1 Score: 420 bits (1080), Expect = 4.08e-146
Identity = 225/309 (72.82%), Postives = 231/309 (74.76%), Query Frame = 0

Query: 3   VGSVCVWPSMKQLSFRKDLLYGVMRLLSIPLKTLRGASRSLKVAQFCSVVNMSSTLQIEL 62
           V SVCVWPS+KQLSFRKDLLYGVMRLLS PLKTLRGASRSLKVAQFCSVVNMSSTLQIEL
Sbjct: 20  VRSVCVWPSVKQLSFRKDLLYGVMRLLSTPLKTLRGASRSLKVAQFCSVVNMSSTLQIEL 79

Query: 63  V----------------------------------------------------------- 122
           V                                                           
Sbjct: 80  VPCLRDNYAYLLHDVDTGTVGVVDPSEALPVIDALSKKNRNLTYILNTHHHHDHTGGNEE 139

Query: 123 ---------IGSGIDSDRIPGIDIALKDGDKWMFAGHEVQVMETPGHTRGHISFYFPGSA 182
                    IGSGIDSDRIPGIDI+LKDGDKWMFAGHEV VMETPGHTRGHISFYFPGSA
Sbjct: 140 LKARYGAKVIGSGIDSDRIPGIDISLKDGDKWMFAGHEVHVMETPGHTRGHISFYFPGSA 199

Query: 183 AIFTGDTLFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFAMSIEPK 242
           AIFTGDTLFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFA+SIEPK
Sbjct: 200 AIFTGDTLFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFALSIEPK 259

BLAST of Cp4.1LG03g13260 vs. ExPASy TrEMBL
Match: A0A6J1I7H0 (probable hydroxyacylglutathione hydrolase 2, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111470704 PE=3 SV=1)

HSP 1 Score: 418 bits (1075), Expect = 2.35e-145
Identity = 224/309 (72.49%), Postives = 231/309 (74.76%), Query Frame = 0

Query: 3   VGSVCVWPSMKQLSFRKDLLYGVMRLLSIPLKTLRGASRSLKVAQFCSVVNMSSTLQIEL 62
           V SVCVWPS+KQLSFRKDLLYGVMRLLS PLKTLRGASRSLKVAQFCSVVNMSSTLQIEL
Sbjct: 20  VRSVCVWPSVKQLSFRKDLLYGVMRLLSTPLKTLRGASRSLKVAQFCSVVNMSSTLQIEL 79

Query: 63  V----------------------------------------------------------- 122
           V                                                           
Sbjct: 80  VPCLRDNYAYLLHDVDTGTVGVVDPSEALPVIDALTKKNRNLTYILNTHHHDDHTGGNEE 139

Query: 123 ---------IGSGIDSDRIPGIDIALKDGDKWMFAGHEVQVMETPGHTRGHISFYFPGSA 182
                    IGSGID D IPGIDI L+DGDKWMFAGHEV VMETPGHTRGHISFYFPGSA
Sbjct: 140 LKARYGAKVIGSGIDRDMIPGIDITLRDGDKWMFAGHEVHVMETPGHTRGHISFYFPGSA 199

Query: 183 AIFTGDTLFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFAMSIEPK 242
           AIFTGDTLFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFA+SIEPK
Sbjct: 200 AIFTGDTLFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFALSIEPK 259

BLAST of Cp4.1LG03g13260 vs. TAIR 10
Match: AT1G06130.1 (glyoxalase 2-4 )

HSP 1 Score: 334.0 bits (855), Expect = 1.0e-91
Identity = 173/310 (55.81%), Postives = 207/310 (66.77%), Query Frame = 0

Query: 2   MVGSVCVWPSMKQLSFRKDLLYGVMRLLSIPLKTLRGASRSLKVAQFCSVVNMSSTLQIE 61
           +V   CV P ++QL  RK L+ GVM+L S PL+TLR A +S+++++FCSV N+SS+LQIE
Sbjct: 20  LVSQPCVRPCVRQLHVRKGLVSGVMKLFSSPLRTLRDAGKSVRISRFCSVSNVSSSLQIE 79

Query: 62  L----------------------------------------------------------- 121
           L                                                           
Sbjct: 80  LVPCLTDNYAYILHDEDTGTVGVVDPSEAVPVMDALQKNSRNLTYILNTHHHYDHTGGNL 139

Query: 122 ---------VIGSGIDSDRIPGIDIALKDGDKWMFAGHEVQVMETPGHTRGHISFYFPGS 181
                    VIGS  D DRIPGID+ALKD DKWMFAGHEV +METPGHTRGHISFYFPG+
Sbjct: 140 ELKDRYGAKVIGSAADRDRIPGIDVALKDADKWMFAGHEVHIMETPGHTRGHISFYFPGA 199

Query: 182 AAIFTGDTLFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFAMSIEP 241
            AIFTGDTLFSLSCGKLFEGTPEQML+SL++I +LPDDT+++CGHEYTLSNSKFA+SIEP
Sbjct: 200 RAIFTGDTLFSLSCGKLFEGTPEQMLASLQRIIALPDDTSVYCGHEYTLSNSKFALSIEP 259

Query: 242 KNEALKSYASQVANLRSKGLPTIPATLKMEKMCNPFLRTSSIEIRQSLNVQNTADDAEVL 244
            NE L+SYA+ VA LR K LPTIP T+KMEK CNPFLRT + +IR++L +  TAD+AE L
Sbjct: 260 TNEVLQSYAAYVAELRDKKLPTIPTTMKMEKACNPFLRTENTDIRRALGIPETADEAEAL 319

BLAST of Cp4.1LG03g13260 vs. TAIR 10
Match: AT1G06130.2 (glyoxalase 2-4 )

HSP 1 Score: 334.0 bits (855), Expect = 1.0e-91
Identity = 173/310 (55.81%), Postives = 207/310 (66.77%), Query Frame = 0

Query: 2   MVGSVCVWPSMKQLSFRKDLLYGVMRLLSIPLKTLRGASRSLKVAQFCSVVNMSSTLQIE 61
           +V   CV P ++QL  RK L+ GVM+L S PL+TLR A +S+++++FCSV N+SS+LQIE
Sbjct: 19  LVSQPCVRPCVRQLHVRKGLVSGVMKLFSSPLRTLRDAGKSVRISRFCSVSNVSSSLQIE 78

Query: 62  L----------------------------------------------------------- 121
           L                                                           
Sbjct: 79  LVPCLTDNYAYILHDEDTGTVGVVDPSEAVPVMDALQKNSRNLTYILNTHHHYDHTGGNL 138

Query: 122 ---------VIGSGIDSDRIPGIDIALKDGDKWMFAGHEVQVMETPGHTRGHISFYFPGS 181
                    VIGS  D DRIPGID+ALKD DKWMFAGHEV +METPGHTRGHISFYFPG+
Sbjct: 139 ELKDRYGAKVIGSAADRDRIPGIDVALKDADKWMFAGHEVHIMETPGHTRGHISFYFPGA 198

Query: 182 AAIFTGDTLFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFAMSIEP 241
            AIFTGDTLFSLSCGKLFEGTPEQML+SL++I +LPDDT+++CGHEYTLSNSKFA+SIEP
Sbjct: 199 RAIFTGDTLFSLSCGKLFEGTPEQMLASLQRIIALPDDTSVYCGHEYTLSNSKFALSIEP 258

Query: 242 KNEALKSYASQVANLRSKGLPTIPATLKMEKMCNPFLRTSSIEIRQSLNVQNTADDAEVL 244
            NE L+SYA+ VA LR K LPTIP T+KMEK CNPFLRT + +IR++L +  TAD+AE L
Sbjct: 259 TNEVLQSYAAYVAELRDKKLPTIPTTMKMEKACNPFLRTENTDIRRALGIPETADEAEAL 318

BLAST of Cp4.1LG03g13260 vs. TAIR 10
Match: AT2G31350.2 (glyoxalase 2-5 )

HSP 1 Score: 324.3 bits (830), Expect = 8.2e-89
Identity = 170/302 (56.29%), Postives = 207/302 (68.54%), Query Frame = 0

Query: 10  PSMKQLSFRKDLLYGVMRLLSIPLKTLRGASRSLKVAQFCSVVNMSSTLQIEL------- 69
           P ++QL+ RK L+  VM+L+S PL+TLRGA +S++V++FCSV N+SS LQIEL       
Sbjct: 23  PCVRQLNIRKSLVCRVMKLVSSPLRTLRGAGKSIRVSKFCSVSNVSS-LQIELVPCLKDN 82

Query: 70  ------------------------------------------------------------ 129
                                                                       
Sbjct: 83  YAYILHDEDTGTVGVVDPSEAEPIIDSLKRSGRNLTYILNTHHHYDHTGGNLELKDRYGA 142

Query: 130 -VIGSGIDSDRIPGIDIALKDGDKWMFAGHEVQVMETPGHTRGHISFYFPGSAAIFTGDT 189
            VIGS +D DRIPGID+ALKDGDKWMFAGHEV VM+TPGHT+GHIS YFPGS AIFTGDT
Sbjct: 143 KVIGSAMDKDRIPGIDMALKDGDKWMFAGHEVHVMDTPGHTKGHISLYFPGSRAIFTGDT 202

Query: 190 LFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFAMSIEPKNEALKSY 244
           +FSLSCGKLFEGTP+QML+SL+KITSLPDDT+I+CGHEYTLSNSKFA+S+EP NE L+SY
Sbjct: 203 MFSLSCGKLFEGTPKQMLASLQKITSLPDDTSIYCGHEYTLSNSKFALSLEPNNEVLQSY 262

BLAST of Cp4.1LG03g13260 vs. TAIR 10
Match: AT2G31350.1 (glyoxalase 2-5 )

HSP 1 Score: 324.3 bits (830), Expect = 8.2e-89
Identity = 170/302 (56.29%), Postives = 207/302 (68.54%), Query Frame = 0

Query: 10  PSMKQLSFRKDLLYGVMRLLSIPLKTLRGASRSLKVAQFCSVVNMSSTLQIEL------- 69
           P ++QL+ RK L+  VM+L+S PL+TLRGA +S++V++FCSV N+SS LQIEL       
Sbjct: 24  PCVRQLNIRKSLVCRVMKLVSSPLRTLRGAGKSIRVSKFCSVSNVSS-LQIELVPCLKDN 83

Query: 70  ------------------------------------------------------------ 129
                                                                       
Sbjct: 84  YAYILHDEDTGTVGVVDPSEAEPIIDSLKRSGRNLTYILNTHHHYDHTGGNLELKDRYGA 143

Query: 130 -VIGSGIDSDRIPGIDIALKDGDKWMFAGHEVQVMETPGHTRGHISFYFPGSAAIFTGDT 189
            VIGS +D DRIPGID+ALKDGDKWMFAGHEV VM+TPGHT+GHIS YFPGS AIFTGDT
Sbjct: 144 KVIGSAMDKDRIPGIDMALKDGDKWMFAGHEVHVMDTPGHTKGHISLYFPGSRAIFTGDT 203

Query: 190 LFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFAMSIEPKNEALKSY 244
           +FSLSCGKLFEGTP+QML+SL+KITSLPDDT+I+CGHEYTLSNSKFA+S+EP NE L+SY
Sbjct: 204 MFSLSCGKLFEGTPKQMLASLQKITSLPDDTSIYCGHEYTLSNSKFALSLEPNNEVLQSY 263

BLAST of Cp4.1LG03g13260 vs. TAIR 10
Match: AT2G43430.2 (glyoxalase 2-1 )

HSP 1 Score: 321.2 bits (822), Expect = 7.0e-88
Identity = 165/308 (53.57%), Postives = 204/308 (66.23%), Query Frame = 0

Query: 4   GSVCVWPSMKQLSFRKDLLYGVMRLLSIPLKTLRGASRSLKVAQFCSVVNMSSTLQIEL- 63
           G +CVWP ++QL  RK LLYGVM LLS+PLKTLRGA ++LK+  FCS+ NM S+L+IEL 
Sbjct: 6   GQLCVWPGLRQLCLRKSLLYGVMWLLSMPLKTLRGARKTLKITHFCSISNMPSSLKIELV 65

Query: 64  ------------------------------------------------------------ 123
                                                                       
Sbjct: 66  PCSKDNYAYLLHDEDTGTVGVVDPSEAAPVIEALSRKNWNLTYILNTHHHDDHIGGNAEL 125

Query: 124 -------VIGSGIDSDRIPGIDIALKDGDKWMFAGHEVQVMETPGHTRGHISFYFPGSAA 183
                  VIGS +D DRIPGIDI LKD DKWMFAGHEV++++TPGHT+GHISFYFPGSA 
Sbjct: 126 KERYGAKVIGSAVDKDRIPGIDILLKDSDKWMFAGHEVRILDTPGHTQGHISFYFPGSAT 185

Query: 184 IFTGDTLFSLSCGKLFEGTPEQMLSSLKKITSLPDDTNIFCGHEYTLSNSKFAMSIEPKN 243
           IFTGD ++SLSCG L EGTPEQMLSSL+KI SLPDDTNI+CG E T  N KFA+S+EPKN
Sbjct: 186 IFTGDLIYSLSCGTLSEGTPEQMLSSLQKIVSLPDDTNIYCGRENTAGNLKFALSVEPKN 245

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LDW81.5e-9055.81Probable hydroxyacylglutathione hydrolase 2, chloroplastic OS=Arabidopsis thalia... [more]
Q9SID31.2e-8756.29Hydroxyacylglutathione hydrolase 2, mitochondrial OS=Arabidopsis thaliana OX=370... [more]
O244959.8e-8753.57Hydroxyacylglutathione hydrolase 1, mitochondrial OS=Arabidopsis thaliana OX=370... [more]
A6WXE02.9e-4648.62Hydroxyacylglutathione hydrolase OS=Ochrobactrum anthropi (strain ATCC 49188 / D... [more]
Q5LNN52.4e-4547.51Hydroxyacylglutathione hydrolase OS=Ruegeria pomeroyi (strain ATCC 700808 / DSM ... [more]
Match NameE-valueIdentityDescription
XP_023528264.16.50e-15777.99probable hydroxyacylglutathione hydrolase 2, chloroplastic isoform X1 [Cucurbita... [more]
XP_022980059.11.86e-15677.67probable hydroxyacylglutathione hydrolase 2, chloroplastic [Cucurbita maxima][more]
XP_022956058.15.32e-15677.35probable hydroxyacylglutathione hydrolase 2, chloroplastic [Cucurbita moschata][more]
KAG6581991.11.07e-15577.02putative hydroxyacylglutathione hydrolase 2, chloroplastic, partial [Cucurbita a... [more]
KAG7018412.15.98e-15577.10putative hydroxyacylglutathione hydrolase 2, chloroplastic, partial [Cucurbita a... [more]
Match NameE-valueIdentityDescription
A0A6J1IYA29.00e-15777.67probable hydroxyacylglutathione hydrolase 2, chloroplastic OS=Cucurbita maxima O... [more]
A0A6J1GVR02.58e-15677.35probable hydroxyacylglutathione hydrolase 2, chloroplastic OS=Cucurbita moschata... [more]
A0A1S3BZ301.50e-14873.79probable hydroxyacylglutathione hydrolase 2, chloroplastic OS=Cucumis melo OX=36... [more]
A0A0A0KYR24.08e-14672.82Lactamase_B domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G009870... [more]
A0A6J1I7H02.35e-14572.49probable hydroxyacylglutathione hydrolase 2, chloroplastic OS=Cucurbita maxima O... [more]
Match NameE-valueIdentityDescription
AT1G06130.11.0e-9155.81glyoxalase 2-4 [more]
AT1G06130.21.0e-9155.81glyoxalase 2-4 [more]
AT2G31350.28.2e-8956.29glyoxalase 2-5 [more]
AT2G31350.18.2e-8956.29glyoxalase 2-5 [more]
AT2G43430.27.0e-8853.57glyoxalase 2-1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001279Metallo-beta-lactamaseSMARTSM00849Lactamase_B_5acoord: 53..158
e-value: 1.6E-5
score: 34.3
IPR001279Metallo-beta-lactamasePFAMPF00753Lactamase_Bcoord: 73..158
e-value: 4.6E-7
score: 30.0
IPR017782Hydroxyacylglutathione hydrolaseTIGRFAMTIGR03413TIGR03413coord: 67..243
e-value: 2.2E-67
score: 225.0
IPR032282Hydroxyacylglutathione hydrolase, C-terminal domainPFAMPF16123HAGH_Ccoord: 159..243
e-value: 2.6E-25
score: 88.7
IPR036866Ribonuclease Z/Hydroxyacylglutathione hydrolase-likeGENE3D3.60.15.10coord: 45..243
e-value: 6.6E-71
score: 240.6
IPR036866Ribonuclease Z/Hydroxyacylglutathione hydrolase-likeSUPERFAMILY56281Metallo-hydrolase/oxidoreductasecoord: 65..243
NoneNo IPR availablePANTHERPTHR11935:SF143HYDROXYACYLGLUTATHIONE HYDROLASEcoord: 5..63
coord: 63..243
NoneNo IPR availablePANTHERPTHR11935BETA LACTAMASE DOMAINcoord: 5..63
coord: 63..243
IPR035680Hydroxyacylglutathione hydrolase, MBL domainCDDcd07723hydroxyacylglutathione_hydrolase_MBL-foldcoord: 63..158
e-value: 1.22256E-52
score: 166.096

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG03g13260.1Cp4.1LG03g13260.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019243 methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione
molecular_function GO:0004416 hydroxyacylglutathione hydrolase activity
molecular_function GO:0046872 metal ion binding