Cp4.1LG03g05790 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG03g05790
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionRING/FYVE/PHD zinc finger superfamily protein
LocationCp4.1LG03: 4574406 .. 4579285 (+)
RNA-Seq ExpressionCp4.1LG03g05790
SyntenyCp4.1LG03g05790
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCCCCCGCTTTGCGGCTGGAAGAATCTCTCGCGTTTCTCCTTCTCCCGCTACATTCTACAGAGAGGGAAGAACAACAAAAAAAACGCACAAACTTTGCTTCTCTCGATCCATTTTCGCTACAAATAATCTGTTGTTTGTTGATTTGGGTTACTGAAACTATCAAATTTTCTTTGAAATGTTAATGGAATTATAAGAACACTCCATCTCTTGCTTCCTCTTTCAAAAATCAGCAGCAGGAGGAAAAGAAGGCTCATATTGTTTGTTTCTTCTATCGCACTAATTCTATTCCCATTTTCGTAAGCATCTGTGTTTCCTCTTTTTCTCTTGCATTTAATCTTAGTTCGTCTGCTGTTCATAAGTTTTTGTCTGCAATGTGTTTGTTCATTTGCTAATCATGTTGGTAGCTGTTTGAAGGTTGCTGCCATTCTCTCTTTTTCTGCATTTTCGTTAATTTTAGGCTTTTTCTCGATCTGGATTGCTGTTTTCTTGAATTTCTTCGGGTTTGGTTTCTGAGTTTTCTTTTTGTTTGGTTTATACCATTTAATCGTATTGATTTAGTCTTGCTTGGCTTTTGATGTTTTGAGTTGTCATTTTCTCTTACATTCCTACATTTTTGCAAGATTTTTGTAACATCTTTCGTTCGATTGCTGCTAAAACTCGGTTAGTTTAACTATGAATAATGAGTTCAAACGTCTGAATCTGTTCAGAGGTCTTAGGAACTAAGTTTTGGGGGCAAAATCGTTGCAGTTTGTTCATAGTCCATAGGGCTCTTCCTACTAATTCTTGAGGAGGCTTTGCCTGTTAGAAGACCAATCTCCAGGGATGATAAGTATTTTAGAGTATTAAGTTTTCAAAATCAAGGTTGGAACCTTTAACTAAATCGAGTTGACTAAATTGTTCTTAGAGCATTGATGTAACTTTTTGAGTATCAGTGAAATGTCTCTTTTTTTCCTCCTAAAAGAGACAAAAAGGATAACTGTAAGGAGCGTTTGGTTTTTCATGGCTCATTTAACATTCAAATTTTTGTTCCCCTTCTCTTTTCTCCTGCAACTTATGATTATTTATCTTCAGGTGAATTCAATGATTCTTTCCTCTTCTTTTTATTTTTATTTTTAATTTTTAAAGAACTGGGGAATCAGGATTTCATTCCTTGCATACTCATGGCCCACTTCATTGATCTTCGAGAAGCTTTGCCCACCCCAAAGCCAACTTTAGAAAACAACAAGGATTTCAAATTAGTAATTTTTTCACCTAGGAATTGGACTCATGTTTTTTGAGGATATATGTAACGGCCCAAGCCCAAGCCCACCGCTAGCCGATATTGTCCTTTTTGGGATTTTCCTTTCGGGTTTCTCCTCAAGGTTTTTAAAACGCGTATGCTAGGGAGAAGTTTCCACACCCTTGTAAAGAATGCTTCGTTCTCCTCCTCAACCGATGTGGGATCTCACAATCCACCCCCCTTCAGGGCTCAGCGTCCTCGTTGGCACTCGTTCCGTTCTCCAATCGATTCGTTGTAGGACCCAATCTCCTTGTTGGCACACTGCCTTGTGTCCACTTCCCTTCGGGGCTCAGTCTCCTCACTAGCACATCACCTAGTGTCTGGCTCTGATACCATTTGTGACAACCCAAGTCCACCGCTAGCAGATATTGTCCTTTTTGGGCTTTCTCTTTCGGGCTTCCGCTCAAGTTTTTTAAAATGCGTATGCTAGGGAGAAGTTTCCACCCTTTATAAAGAATGCTTCGTTCTCCTTCCCAACCGAGATGGAATCTCACAATATCTTCTGACATCCCAATTCTAAGGACCTTACCAGTCACACCATCACCCCATGGGTTGTAAACTCCTAAAGTTTCAAAATTTCTATTCTTTCTTTATTTTCTGCTAGATCTTTGAATTATACTATGCTGAGAAACAATCTGAATTGTCATGGATGTTGTTCACTGCAGTAAACAAAATTGGTCTACATAGTTGTAAATGTTGTTTGGCTTATTTTATATGATTTTATTGACATTCATTCATTCCAATGTTTACTGGGTCATTGAAGAATGGGGGAACATTTTGTGCTGTTGGTAGATCAGTTGCTCACAGAATCAAACCTCGAAGCTACTATTGAGAGAAAAAACAGAAATTGTCGTCCTATGGCCTCAACGAGTGCAGATAACATGATATCTTCCTTAAATATTGATGTTGAATTCATGTCACCTTCAAGTAAACTTGTTCAGTGCAGAATTTGTCATGATGAGGACGATGAATCAAAAATGGAGACACCATGCTCTTGCTGTGGAAGCTTGAAGGTTAGATTTGGTATACTCAACACCAATTAGTGCTACTTAATCAATAAAACTCAGATAGCTTTTCTTTGAATCCCTCGTTTCCAAATTATGACTGACTAAACTGGTTTCAACAATGTTCCTGTCGATATTTTCTGCACGTTTCCAAATTATATGACTGACTAAACTGTTTTCAACAATGTTCCTATCGATATTTTCTGCATTTAGTTTCGGTCCAACTTTATCCTTTCCCCCAAATCTTTGCCCCGGATGTTATAATATGCTATAAACACTTGCTAAGTCAGGTCCCTTGGTTTATATTTACTTGTTGCTAGACTGAAAAATGTTAGAATCAGATTCATATCTTTATTCCTCACTGTGTTCGTTTAGATGGTTCACCTTGATAATTAGCTTCCAGTTAGTGTTGATAATTGCAGTATGCTCATCGCAAGTGCGTTCAGCGGTGGTGCAACGAGAAAGGCAATACAATCTGTGAAATTTGCCACCAGGTCTGTTGTTTTTGTTCAAATAAATGAAAATTTCTTTAATACATGACTAACTTTATGGTCGTAAGAAGTCGTTTTACTTATACTGCAGGACTTTGAGCCAGGGTATACAGCACCACTCCCTGTATTATATTATGGAGATATTACTTCACCAGTTCATTTCAGGTAAACCATTTACCTCTCTCCCCACAATGCAAGTTTCTCTAGTTTGCATTGTATATTCACTTATTGTTCGTTTATTTTCTCGACACTATTGATATAACATCGCTGTCGTCGAATTGTGTTCTTAGGGAAAGTTGGGAGATGTCTAGACTGAACCTGCATGTACCTGCAGGAATGCCTGATCACGAGTATCCTGATTCTGACTTTGATGAATTCTTCACTCCTTCTCCAAGAAGCATATTGTGCTGCCGCATGGTTGCAGTAACTGTATTCCCTACCTCTTCATTTTACTTCATTTCCAGTTTCCTTATTTCTGTAAACAAATTTACATTGATTGATAATTGGCTTTGCCTTCTGCACATAAAAAAAGTATTAAATGTTCTTGCTCGTTGATTCTTTTTCATTCACTTGTGAAGGGAGGAAAGCAAACATAGTTTTTGGTTTAAGTTCGAGCATTCGTTCTTATAAGATTTTATTTTAGTCTTTGAACTTTCTATTAAGACTTTGAACATTCGTTCTTCTGCATGAACAAGTGTTTGATTCACGATTAGTAAGTTAAGTATTGGGTCTTCAAGCATCAACGAAAAGAATTTAAGTAGTCATGTAATCTAAGTTTCGGAGACGAAATAGAGTATTAGAAGTCGATGGATTAAAATTTAACGTTTTGAAGCTTAAGAATGAAAATGAAACGAGCATCAAAGTTCATATCTATAATACGATTTAAACCTGTATTCGCCATGCTCATATTTGACCAACAATCCCGAGTTTGAAACTTCTAATCGTATCTGTATGATGCAGTTCATCGTTCTTCTCGTTTTGCGCCATACGCTACCAATTGTAATCAGTGGAGCTGGAGAGTATTCATTGACATTGTTAATGGTAGGCTTGGTTTCTCTGTTTCCCTTTCTACTTTTACTTTGGCCCTCTTCCATTGTTGAGACCAATGAAGAACCATAAGTTCATGTGCTGTATGTTTCAGCTGCTGATCTTGAGAATTGTTGGAATCCTTCTACCAATCTATGTCATGGTTAGAGCATTTACTTCCATTCAACGACGGCGTCATTATCAGGTTAGTGAGGCAGTTTATGTCTGGATGATTCGTTTCGTCGTAAGAACTGCTTGGATTTTCGAAGAGGTCGATACAGAATAGATATGAATTTTGTTGGGCTATTCTGTTATACGGTGTCACATATGTAGTTGAAGTGCATATAAGGACACCCATGATCAACTTAAACGTAACTCAACTAATTAAAGCATATATCTTCGATCGATGATTGTACTGTTACCAACCATGATGAACATACGCTAGACAGTGCACATTCCTGAAAACTTGCTCTGGTTATCTTTTCTATTTGAGCTTGATGTCTAGCAGTTGTTTACATTCCCTTGATACGACGTTTGAGTATACTAGTATGCTTTTGGTAGGATCCCCGTCTTCGCCTCGCAACACCAGAGGACGAAAATGACTCGACAAACCATGCTCAATCGCGCTTCATCCACGTGAGATAGCAGTACATTCACCTCGTCTCTAATACGACGAAACCAACGTAAGCATAACGACGAGATGGAAACTGGTTGGATGTAACGCAATAGAAGGTCATCAACGCCATTCATGGTCCTTGCAGCGAGACTCGACCACGACCACTACAACAATCACGACCCAGTTATTTTCTGCAGTGAAGTTCATCATCTGTGGGAAGGCAGCTGAAACTTCTTTGGTTAAAATCAAACTTTTCATGCAATGGAATCGTTGGCTCACTTCACTCACTACTCTTTAATGTATAAAGTGTGTAATGGTTAGGAAGGGAAATCAGCATTGCTTTCATATTCGCTCTTAGAAATTATAAGATAGTTGTAATTAAATTATGTTCTTACACTGACAAACAAACTAAATTGTTGGGAAGTTTAAACTTCCACCTTCATACGTCGTTGAACTCAAAATA

mRNA sequence

GCCCCCGCTTTGCGGCTGGAAGAATCTCTCGCGTTTCTCCTTCTCCCGCTACATTCTACAGAGAGGGAAGAACAACAAAAAAAACGCACAAACTTTGCTTCTCTCGATCCATTTTCGCTACAAATAATCTGTTGTTTGTTGATTTGGGTTACTGAAACTATCAAATTTTCTTTGAAATGTTAATGGAATTATAAGAACACTCCATCTCTTGCTTCCTCTTTCAAAAATCAGCAGCAGGAGGAAAAGAAGGCTCATATTGTTTGTTTCTTCTATCGCACTAATTCTATTCCCATTTTCAATGGGGGAACATTTTGTGCTGTTGGTAGATCAGTTGCTCACAGAATCAAACCTCGAAGCTACTATTGAGAGAAAAAACAGAAATTGTCGTCCTATGGCCTCAACGAGTGCAGATAACATGATATCTTCCTTAAATATTGATGTTGAATTCATGTCACCTTCAAGTAAACTTGTTCAGTGCAGAATTTGTCATGATGAGGACGATGAATCAAAAATGGAGACACCATGCTCTTGCTGTGGAAGCTTGAAGTATGCTCATCGCAAGTGCGTTCAGCGGTGGTGCAACGAGAAAGGCAATACAATCTGTGAAATTTGCCACCAGGACTTTGAGCCAGGGTATACAGCACCACTCCCTGTATTATATTATGGAGATATTACTTCACCAGTTCATTTCAGGGAAAGTTGGGAGATGTCTAGACTGAACCTGCATGTACCTGCAGGAATGCCTGATCACGAGTATCCTGATTCTGACTTTGATGAATTCTTCACTCCTTCTCCAAGAAGCATATTGTGCTGCCGCATGGTTGCAGTAACTGTATTCCCTACCTCTTCATTTTACTTCATTTCCAGTTTCCTTATTTCTTTCATCGTTCTTCTCGTTTTGCGCCATACGCTACCAATTGTAATCAGTGGAGCTGGAGAGTATTCATTGACATTGTTAATGCTGCTGATCTTGAGAATTGTTGGAATCCTTCTACCAATCTATGTCATGGTTAGAGCATTTACTTCCATTCAACGACGGCGTCATTATCAGGATCCCCGTCTTCGCCTCGCAACACCAGAGGACGAAAATGACTCGACAAACCATGCTCAATCGCGCTTCATCCACGTGAGATAGCAGTACATTCACCTCGTCTCTAATACGACGAAACCAACGTAAGCATAACGACGAGATGGAAACTGGTTGGATGTAACGCAATAGAAGGTCATCAACGCCATTCATGGTCCTTGCAGCGAGACTCGACCACGACCACTACAACAATCACGACCCAGTTATTTTCTGCAGTGAAGTTCATCATCTGTGGGAAGGCAGCTGAAACTTCTTTGGTTAAAATCAAACTTTTCATGCAATGGAATCGTTGGCTCACTTCACTCACTACTCTTTAATGTATAAAGTGTGTAATGGTTAGGAAGGGAAATCAGCATTGCTTTCATATTCGCTCTTAGAAATTATAAGATAGTTGTAATTAAATTATGTTCTTACACTGACAAACAAACTAAATTGTTGGGAAGTTTAAACTTCCACCTTCATACGTCGTTGAACTCAAAATA

Coding sequence (CDS)

ATGGGGGAACATTTTGTGCTGTTGGTAGATCAGTTGCTCACAGAATCAAACCTCGAAGCTACTATTGAGAGAAAAAACAGAAATTGTCGTCCTATGGCCTCAACGAGTGCAGATAACATGATATCTTCCTTAAATATTGATGTTGAATTCATGTCACCTTCAAGTAAACTTGTTCAGTGCAGAATTTGTCATGATGAGGACGATGAATCAAAAATGGAGACACCATGCTCTTGCTGTGGAAGCTTGAAGTATGCTCATCGCAAGTGCGTTCAGCGGTGGTGCAACGAGAAAGGCAATACAATCTGTGAAATTTGCCACCAGGACTTTGAGCCAGGGTATACAGCACCACTCCCTGTATTATATTATGGAGATATTACTTCACCAGTTCATTTCAGGGAAAGTTGGGAGATGTCTAGACTGAACCTGCATGTACCTGCAGGAATGCCTGATCACGAGTATCCTGATTCTGACTTTGATGAATTCTTCACTCCTTCTCCAAGAAGCATATTGTGCTGCCGCATGGTTGCAGTAACTGTATTCCCTACCTCTTCATTTTACTTCATTTCCAGTTTCCTTATTTCTTTCATCGTTCTTCTCGTTTTGCGCCATACGCTACCAATTGTAATCAGTGGAGCTGGAGAGTATTCATTGACATTGTTAATGCTGCTGATCTTGAGAATTGTTGGAATCCTTCTACCAATCTATGTCATGGTTAGAGCATTTACTTCCATTCAACGACGGCGTCATTATCAGGATCCCCGTCTTCGCCTCGCAACACCAGAGGACGAAAATGACTCGACAAACCATGCTCAATCGCGCTTCATCCACGTGAGATAG

Protein sequence

MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMSPSSKLVQCRICHDEDDESKMETPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVLYYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVTVFPTSSFYFISSFLISFIVLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRAFTSIQRRRHYQDPRLRLATPEDENDSTNHAQSRFIHVR
Homology
BLAST of Cp4.1LG03g05790 vs. ExPASy Swiss-Prot
Match: Q5T0T0 (E3 ubiquitin-protein ligase MARCHF8 OS=Homo sapiens OX=9606 GN=MARCHF8 PE=1 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 3.2e-09
Identity = 28/60 (46.67%), Postives = 39/60 (65.00%), Query Frame = 0

Query: 51  MSPSSKLVQCRICHDE-DDESKMETPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDF 110
           ++PSS+ + CRICH E DDES + TPC C GSL + H+ C+Q+W        CE+C  +F
Sbjct: 72  ITPSSQDI-CRICHCEGDDESPLITPCHCTGSLHFVHQACLQQWIKSSDTRCCELCKYEF 130

BLAST of Cp4.1LG03g05790 vs. ExPASy Swiss-Prot
Match: Q5XH39 (E3 ubiquitin-protein ligase MARCHF8 OS=Xenopus laevis OX=8355 GN=marchf8 PE=2 SV=2)

HSP 1 Score: 63.2 bits (152), Expect = 5.5e-09
Identity = 28/60 (46.67%), Postives = 39/60 (65.00%), Query Frame = 0

Query: 51  MSPSSKLVQCRICHDE-DDESKMETPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDF 110
           ++PSS+ + CRICH E DDES + TPC C GSL + H+ C+Q+W        CE+C  +F
Sbjct: 45  VTPSSQDI-CRICHCEGDDESPLITPCHCTGSLHFVHQACLQQWIKSSDTRCCELCKFEF 103

BLAST of Cp4.1LG03g05790 vs. ExPASy Swiss-Prot
Match: Q0VD59 (E3 ubiquitin-protein ligase MARCHF8 OS=Bos taurus OX=9913 GN=MARCHF8 PE=2 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 7.2e-09
Identity = 27/60 (45.00%), Postives = 39/60 (65.00%), Query Frame = 0

Query: 51  MSPSSKLVQCRICHDE-DDESKMETPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDF 110
           ++PS++ + CRICH E DDES + TPC C GSL + H+ C+Q+W        CE+C  +F
Sbjct: 70  VTPSNQDI-CRICHCEGDDESPLITPCRCTGSLHFVHQTCLQQWIKSSDTRCCELCKYEF 128

BLAST of Cp4.1LG03g05790 vs. ExPASy Swiss-Prot
Match: Q9DBD2 (E3 ubiquitin-protein ligase MARCHF8 OS=Mus musculus OX=10090 GN=Marchf8 PE=2 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 7.2e-09
Identity = 27/60 (45.00%), Postives = 39/60 (65.00%), Query Frame = 0

Query: 51  MSPSSKLVQCRICHDE-DDESKMETPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDF 110
           ++PS++ + CRICH E DDES + TPC C GSL + H+ C+Q+W        CE+C  +F
Sbjct: 68  VTPSNQDI-CRICHCEGDDESPLITPCHCTGSLHFVHQACLQQWIKSSDTRCCELCKYEF 126

BLAST of Cp4.1LG03g05790 vs. ExPASy Swiss-Prot
Match: Q28IK8 (E3 ubiquitin-protein ligase MARCHF8 OS=Xenopus tropicalis OX=8364 GN=marchf8 PE=2 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 9.4e-09
Identity = 36/100 (36.00%), Postives = 52/100 (52.00%), Query Frame = 0

Query: 11  QLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMSPSSKLVQCRICHDE-DDE 70
           +L  E  L  ++ R +   +  + TS     S     V   +PSS+ + CRICH E DDE
Sbjct: 8   KLQNEKTLGHSVSRSSNISKAGSPTSVSAPSSFPRTSV---TPSSQDI-CRICHCEGDDE 67

Query: 71  SKMETPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDF 110
           S + TPC C GSL + H+ C+Q+W        CE+C  +F
Sbjct: 68  SPLITPCHCTGSLHFVHQACLQQWIKSSDTRCCELCKFEF 103

BLAST of Cp4.1LG03g05790 vs. NCBI nr
Match: XP_023528859.1 (uncharacterized protein LOC111791660 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023528860.1 uncharacterized protein LOC111791660 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023528861.1 uncharacterized protein LOC111791660 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 524 bits (1350), Expect = 2.35e-187
Identity = 262/278 (94.24%), Postives = 262/278 (94.24%), Query Frame = 0

Query: 1   MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMSPSSKLVQC 60
           MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMSPSSKLVQC
Sbjct: 1   MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMSPSSKLVQC 60

Query: 61  RICHDEDDESKMETPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120
           RICHDEDDESKMETPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL
Sbjct: 61  RICHDEDDESKMETPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120

Query: 121 YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVTVF 180
           YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVT  
Sbjct: 121 YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVT-- 180

Query: 181 PTSSFYFISSFLISFIVLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRA 240
                         FIVLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRA
Sbjct: 181 --------------FIVLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRA 240

Query: 241 FTSIQRRRHYQDPRLRLATPEDENDSTNHAQSRFIHVR 278
           FTSIQRRRHYQDPRLRLATPEDENDSTNHAQSRFIHVR
Sbjct: 241 FTSIQRRRHYQDPRLRLATPEDENDSTNHAQSRFIHVR 262

BLAST of Cp4.1LG03g05790 vs. NCBI nr
Match: KAG7017957.1 (E3 ubiquitin-protein ligase MARCH8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 520 bits (1338), Expect = 1.11e-184
Identity = 261/278 (93.88%), Postives = 261/278 (93.88%), Query Frame = 0

Query: 1   MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMSPSSKLVQC 60
           MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMSPSSKLVQC
Sbjct: 54  MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMSPSSKLVQC 113

Query: 61  RICHDEDDESKMETPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120
           RICHDEDDESKMETPCSC GSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL
Sbjct: 114 RICHDEDDESKMETPCSCRGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 173

Query: 121 YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVTVF 180
           YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVT  
Sbjct: 174 YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVT-- 233

Query: 181 PTSSFYFISSFLISFIVLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRA 240
                         FIVLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRA
Sbjct: 234 --------------FIVLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRA 293

Query: 241 FTSIQRRRHYQDPRLRLATPEDENDSTNHAQSRFIHVR 278
           FTSIQRRRHYQDPRLRLATPEDENDSTNHAQSRFIHVR
Sbjct: 294 FTSIQRRRHYQDPRLRLATPEDENDSTNHAQSRFIHVR 315

BLAST of Cp4.1LG03g05790 vs. NCBI nr
Match: XP_022984108.1 (uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984109.1 uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984110.1 uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984111.1 uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984112.1 uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984113.1 uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984114.1 uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984115.1 uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984116.1 uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984117.1 uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984118.1 uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984119.1 uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984120.1 uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984122.1 uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984123.1 uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984124.1 uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984125.1 uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984126.1 uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984127.1 uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984129.1 uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima])

HSP 1 Score: 517 bits (1332), Expect = 1.30e-184
Identity = 259/278 (93.17%), Postives = 259/278 (93.17%), Query Frame = 0

Query: 1   MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMSPSSKLVQC 60
           MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFM  SSKLVQC
Sbjct: 1   MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMPSSSKLVQC 60

Query: 61  RICHDEDDESKMETPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120
           RICHDEDDESKME PCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL
Sbjct: 61  RICHDEDDESKMEAPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120

Query: 121 YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVTVF 180
           YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVT  
Sbjct: 121 YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVT-- 180

Query: 181 PTSSFYFISSFLISFIVLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRA 240
                         FIVLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRA
Sbjct: 181 --------------FIVLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRA 240

Query: 241 FTSIQRRRHYQDPRLRLATPEDENDSTNHAQSRFIHVR 278
           FTSIQRRRHYQDPRLRLATPEDENDSTNHAQSRFIHVR
Sbjct: 241 FTSIQRRRHYQDPRLRLATPEDENDSTNHAQSRFIHVR 262

BLAST of Cp4.1LG03g05790 vs. NCBI nr
Match: XP_023528862.1 (uncharacterized protein LOC111791660 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 516 bits (1329), Expect = 3.22e-184
Identity = 258/278 (92.81%), Postives = 258/278 (92.81%), Query Frame = 0

Query: 1   MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMSPSSKLVQC 60
           MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMSPSSKLVQC
Sbjct: 1   MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMSPSSKLVQC 60

Query: 61  RICHDEDDESKMETPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120
           RICHDEDDESKMETPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL
Sbjct: 61  RICHDEDDESKMETPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120

Query: 121 YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVTVF 180
           YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRM      
Sbjct: 121 YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRM------ 180

Query: 181 PTSSFYFISSFLISFIVLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRA 240
                         FIVLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRA
Sbjct: 181 --------------FIVLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRA 240

Query: 241 FTSIQRRRHYQDPRLRLATPEDENDSTNHAQSRFIHVR 278
           FTSIQRRRHYQDPRLRLATPEDENDSTNHAQSRFIHVR
Sbjct: 241 FTSIQRRRHYQDPRLRLATPEDENDSTNHAQSRFIHVR 258

BLAST of Cp4.1LG03g05790 vs. NCBI nr
Match: XP_022935629.1 (uncharacterized protein LOC111442434 isoform X1 [Cucurbita moschata] >XP_022935630.1 uncharacterized protein LOC111442434 isoform X1 [Cucurbita moschata] >XP_022935631.1 uncharacterized protein LOC111442434 isoform X1 [Cucurbita moschata] >XP_022935632.1 uncharacterized protein LOC111442434 isoform X1 [Cucurbita moschata] >XP_022935633.1 uncharacterized protein LOC111442434 isoform X1 [Cucurbita moschata] >XP_022935634.1 uncharacterized protein LOC111442434 isoform X1 [Cucurbita moschata] >XP_022935635.1 uncharacterized protein LOC111442434 isoform X1 [Cucurbita moschata] >XP_022935636.1 uncharacterized protein LOC111442434 isoform X1 [Cucurbita moschata] >XP_022935637.1 uncharacterized protein LOC111442434 isoform X1 [Cucurbita moschata] >XP_022935638.1 uncharacterized protein LOC111442434 isoform X1 [Cucurbita moschata] >XP_022935640.1 uncharacterized protein LOC111442434 isoform X1 [Cucurbita moschata] >XP_022935641.1 uncharacterized protein LOC111442434 isoform X1 [Cucurbita moschata] >XP_022935643.1 uncharacterized protein LOC111442434 isoform X1 [Cucurbita moschata])

HSP 1 Score: 514 bits (1324), Expect = 2.16e-183
Identity = 257/278 (92.45%), Postives = 258/278 (92.81%), Query Frame = 0

Query: 1   MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMSPSSKLVQC 60
           MGEHFVLLVDQLLTESNLE+TIERKNRNCRPMASTSADNMISSLNIDVEFMSPSSKLVQC
Sbjct: 1   MGEHFVLLVDQLLTESNLESTIERKNRNCRPMASTSADNMISSLNIDVEFMSPSSKLVQC 60

Query: 61  RICHDEDDESKMETPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120
           RICHDEDDESKMETPCSCCGSLKYAHR CVQRWCNEKGNTICEICHQDFEPGYTAPLPVL
Sbjct: 61  RICHDEDDESKMETPCSCCGSLKYAHRMCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120

Query: 121 YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVTVF 180
           YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVT  
Sbjct: 121 YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVT-- 180

Query: 181 PTSSFYFISSFLISFIVLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRA 240
                         FIVLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRA
Sbjct: 181 --------------FIVLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRA 240

Query: 241 FTSIQRRRHYQDPRLRLATPEDENDSTNHAQSRFIHVR 278
           FTSIQ RRHYQDPRL LATPEDENDSTNHAQS FIHVR
Sbjct: 241 FTSIQGRRHYQDPRLHLATPEDENDSTNHAQSHFIHVR 262

BLAST of Cp4.1LG03g05790 vs. ExPASy TrEMBL
Match: A0A6J1J9L9 (uncharacterized protein LOC111482515 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111482515 PE=4 SV=1)

HSP 1 Score: 517 bits (1332), Expect = 6.30e-185
Identity = 259/278 (93.17%), Postives = 259/278 (93.17%), Query Frame = 0

Query: 1   MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMSPSSKLVQC 60
           MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFM  SSKLVQC
Sbjct: 1   MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMPSSSKLVQC 60

Query: 61  RICHDEDDESKMETPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120
           RICHDEDDESKME PCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL
Sbjct: 61  RICHDEDDESKMEAPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120

Query: 121 YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVTVF 180
           YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVT  
Sbjct: 121 YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVT-- 180

Query: 181 PTSSFYFISSFLISFIVLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRA 240
                         FIVLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRA
Sbjct: 181 --------------FIVLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRA 240

Query: 241 FTSIQRRRHYQDPRLRLATPEDENDSTNHAQSRFIHVR 278
           FTSIQRRRHYQDPRLRLATPEDENDSTNHAQSRFIHVR
Sbjct: 241 FTSIQRRRHYQDPRLRLATPEDENDSTNHAQSRFIHVR 262

BLAST of Cp4.1LG03g05790 vs. ExPASy TrEMBL
Match: A0A6J1FB67 (uncharacterized protein LOC111442434 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111442434 PE=4 SV=1)

HSP 1 Score: 514 bits (1324), Expect = 1.04e-183
Identity = 257/278 (92.45%), Postives = 258/278 (92.81%), Query Frame = 0

Query: 1   MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMSPSSKLVQC 60
           MGEHFVLLVDQLLTESNLE+TIERKNRNCRPMASTSADNMISSLNIDVEFMSPSSKLVQC
Sbjct: 1   MGEHFVLLVDQLLTESNLESTIERKNRNCRPMASTSADNMISSLNIDVEFMSPSSKLVQC 60

Query: 61  RICHDEDDESKMETPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120
           RICHDEDDESKMETPCSCCGSLKYAHR CVQRWCNEKGNTICEICHQDFEPGYTAPLPVL
Sbjct: 61  RICHDEDDESKMETPCSCCGSLKYAHRMCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120

Query: 121 YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVTVF 180
           YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVT  
Sbjct: 121 YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVT-- 180

Query: 181 PTSSFYFISSFLISFIVLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRA 240
                         FIVLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRA
Sbjct: 181 --------------FIVLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRA 240

Query: 241 FTSIQRRRHYQDPRLRLATPEDENDSTNHAQSRFIHVR 278
           FTSIQ RRHYQDPRL LATPEDENDSTNHAQS FIHVR
Sbjct: 241 FTSIQGRRHYQDPRLHLATPEDENDSTNHAQSHFIHVR 262

BLAST of Cp4.1LG03g05790 vs. ExPASy TrEMBL
Match: A0A6J1J9N6 (uncharacterized protein LOC111482515 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111482515 PE=4 SV=1)

HSP 1 Score: 509 bits (1311), Expect = 8.65e-182
Identity = 255/278 (91.73%), Postives = 255/278 (91.73%), Query Frame = 0

Query: 1   MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMSPSSKLVQC 60
           MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFM  SSKLVQC
Sbjct: 1   MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMPSSSKLVQC 60

Query: 61  RICHDEDDESKMETPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120
           RICHDEDDESKME PCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL
Sbjct: 61  RICHDEDDESKMEAPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120

Query: 121 YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVTVF 180
           YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRM      
Sbjct: 121 YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRM------ 180

Query: 181 PTSSFYFISSFLISFIVLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRA 240
                         FIVLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRA
Sbjct: 181 --------------FIVLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRA 240

Query: 241 FTSIQRRRHYQDPRLRLATPEDENDSTNHAQSRFIHVR 278
           FTSIQRRRHYQDPRLRLATPEDENDSTNHAQSRFIHVR
Sbjct: 241 FTSIQRRRHYQDPRLRLATPEDENDSTNHAQSRFIHVR 258

BLAST of Cp4.1LG03g05790 vs. ExPASy TrEMBL
Match: A0A6J1F655 (uncharacterized protein LOC111442434 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111442434 PE=4 SV=1)

HSP 1 Score: 506 bits (1303), Expect = 1.43e-180
Identity = 253/278 (91.01%), Postives = 254/278 (91.37%), Query Frame = 0

Query: 1   MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMSPSSKLVQC 60
           MGEHFVLLVDQLLTESNLE+TIERKNRNCRPMASTSADNMISSLNIDVEFMSPSSKLVQC
Sbjct: 1   MGEHFVLLVDQLLTESNLESTIERKNRNCRPMASTSADNMISSLNIDVEFMSPSSKLVQC 60

Query: 61  RICHDEDDESKMETPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120
           RICHDEDDESKMETPCSCCGSLKYAHR CVQRWCNEKGNTICEICHQDFEPGYTAPLPVL
Sbjct: 61  RICHDEDDESKMETPCSCCGSLKYAHRMCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120

Query: 121 YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVTVF 180
           YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRM      
Sbjct: 121 YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRM------ 180

Query: 181 PTSSFYFISSFLISFIVLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRA 240
                         FIVLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRA
Sbjct: 181 --------------FIVLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRA 240

Query: 241 FTSIQRRRHYQDPRLRLATPEDENDSTNHAQSRFIHVR 278
           FTSIQ RRHYQDPRL LATPEDENDSTNHAQS FIHVR
Sbjct: 241 FTSIQGRRHYQDPRLHLATPEDENDSTNHAQSHFIHVR 258

BLAST of Cp4.1LG03g05790 vs. ExPASy TrEMBL
Match: A0A0A0LBM8 (RING-CH-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G186720 PE=4 SV=1)

HSP 1 Score: 427 bits (1098), Expect = 2.95e-149
Identity = 218/280 (77.86%), Postives = 235/280 (83.93%), Query Frame = 0

Query: 1   MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSA--DNMISSLNIDVEFMSPSSKLV 60
           MG+HF LLVDQLLTESNLEATIERKNR C PMAST+A  DNMISS NIDVE +SPSS +V
Sbjct: 1   MGDHFGLLVDQLLTESNLEATIERKNRICHPMASTNANADNMISSSNIDVESVSPSS-IV 60

Query: 61  QCRICHDEDDESKMETPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLP 120
           QCRICHDEDD SKMETPCSCCGSLKYAHRKC+QRWCNEKG+TICEICHQDF+PGYT+P P
Sbjct: 61  QCRICHDEDDGSKMETPCSCCGSLKYAHRKCIQRWCNEKGDTICEICHQDFKPGYTSPPP 120

Query: 121 VLYYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVT 180
           V YYGDI SP+HFR SWEMSRLNLHVPAGMPDHEY DSDFD+FF PSPRSILCCR+VAV 
Sbjct: 121 VFYYGDINSPIHFRGSWEMSRLNLHVPAGMPDHEYLDSDFDDFFAPSPRSILCCRVVAVI 180

Query: 181 VFPTSSFYFISSFLISFIVLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMV 240
                           FI LLVLRHTLPIVISGAG YS TLLMLLILRIVGILLPIYVMV
Sbjct: 181 ----------------FIALLVLRHTLPIVISGAGGYSWTLLMLLILRIVGILLPIYVMV 240

Query: 241 RAFTSIQRRRHYQDPRLRLATPEDENDSTNHAQSRFIHVR 278
           +AFT IQRR  YQ PRL+L T  D ++STNH+QS  IH+R
Sbjct: 241 KAFTYIQRRHRYQAPRLQLTTLGDNSESTNHSQSHLIHLR 263

BLAST of Cp4.1LG03g05790 vs. TAIR 10
Match: AT2G01275.1 (RING/FYVE/PHD zinc finger superfamily protein )

HSP 1 Score: 205.7 bits (522), Expect = 4.9e-53
Identity = 121/286 (42.31%), Postives = 167/286 (58.39%), Query Frame = 0

Query: 1   MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMSPSSKLVQC 60
           MG+HFVLLVD+L+TES +EA I+ +N+  +         ++    +++   +    + +C
Sbjct: 1   MGDHFVLLVDRLITESTIEAAIQSRNQMLQANIPVEECTILDDKTLEM-LRNGDLSMAEC 60

Query: 61  RICHDEDDESKMETPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120
           RICHDED +S METPCSC GS+KYAHR+CVQRWCNEKG+T CEICHQ+F+P YTAP P+L
Sbjct: 61  RICHDEDLDSNMETPCSCSGSVKYAHRRCVQRWCNEKGDTTCEICHQEFKPDYTAPPPLL 120

Query: 121 YYGDITSPVHFRESWEMS----RLNLHVPAGMP--DHEYPDSDFDEFFTPSPRSILCCRM 180
             G +  P+HFR +W +S    R    VPA     D  YP S        S  S +CCR 
Sbjct: 121 ELGHV--PLHFRGNWGISQREHRFITVVPADSTFIDQPYPLS--------STTSFICCR- 180

Query: 181 VAVTVFPTSSFYFISSFLISFIVLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPI 240
                          S ++ F+ LL+LRHTLP+V++G+  +   L  LL LRI+GI+LPI
Sbjct: 181 ---------------SLVLIFMALLILRHTLPLVLTGSNLHVFPLFTLLFLRILGIMLPI 240

Query: 241 YVMVRAFTSIQRRRHYQDPRLRLATPEDENDSTN--HAQSRFIHVR 279
           YV+ +A  + +R     D      + ++E DS      QS  I VR
Sbjct: 241 YVVTKAVATCRRHSRTLDTSPSDDSSDEETDSWRLPQTQSYIIGVR 259

BLAST of Cp4.1LG03g05790 vs. TAIR 10
Match: AT2G01275.2 (RING/FYVE/PHD zinc finger superfamily protein )

HSP 1 Score: 205.7 bits (522), Expect = 4.9e-53
Identity = 121/286 (42.31%), Postives = 167/286 (58.39%), Query Frame = 0

Query: 1   MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMSPSSKLVQC 60
           MG+HFVLLVD+L+TES +EA I+ +N+  +         ++    +++   +    + +C
Sbjct: 1   MGDHFVLLVDRLITESTIEAAIQSRNQMLQANIPVEECTILDDKTLEM-LRNGDLSMAEC 60

Query: 61  RICHDEDDESKMETPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120
           RICHDED +S METPCSC GS+KYAHR+CVQRWCNEKG+T CEICHQ+F+P YTAP P+L
Sbjct: 61  RICHDEDLDSNMETPCSCSGSVKYAHRRCVQRWCNEKGDTTCEICHQEFKPDYTAPPPLL 120

Query: 121 YYGDITSPVHFRESWEMS----RLNLHVPAGMP--DHEYPDSDFDEFFTPSPRSILCCRM 180
             G +  P+HFR +W +S    R    VPA     D  YP S        S  S +CCR 
Sbjct: 121 ELGHV--PLHFRGNWGISQREHRFITVVPADSTFIDQPYPLS--------STTSFICCR- 180

Query: 181 VAVTVFPTSSFYFISSFLISFIVLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPI 240
                          S ++ F+ LL+LRHTLP+V++G+  +   L  LL LRI+GI+LPI
Sbjct: 181 ---------------SLVLIFMALLILRHTLPLVLTGSNLHVFPLFTLLFLRILGIMLPI 240

Query: 241 YVMVRAFTSIQRRRHYQDPRLRLATPEDENDSTN--HAQSRFIHVR 279
           YV+ +A  + +R     D      + ++E DS      QS  I VR
Sbjct: 241 YVVTKAVATCRRHSRTLDTSPSDDSSDEETDSWRLPQTQSYIIGVR 259

BLAST of Cp4.1LG03g05790 vs. TAIR 10
Match: AT5G38070.1 (RING/FYVE/PHD zinc finger superfamily protein )

HSP 1 Score: 198.7 bits (504), Expect = 6.0e-51
Identity = 110/264 (41.67%), Postives = 165/264 (62.50%), Query Frame = 0

Query: 1   MGEHFVLLVDQLLTESNLEATIERKNRNCRPMA-STSADNMISSLNIDVEFMSPSSKLVQ 60
           MG+H VL+VD+L ++S L  T+ R +    P A S + D +  S++   +     SK VQ
Sbjct: 1   MGDHLVLVVDRLSSDSGL-GTVNRAD----PKADSVNEDGVSESISAGADLC--ESKFVQ 60

Query: 61  CRICHDEDDESKMETPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPV 120
           CRICHDED+++ M+TPCSC G+LK+AH  CVQRWCNEKG+T+CEIC Q ++PGYTAP  +
Sbjct: 61  CRICHDEDEDTNMDTPCSCSGTLKFAHHNCVQRWCNEKGDTVCEICRQQYKPGYTAPRQL 120

Query: 121 LYYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVTV 180
            +Y  I+  ++F   W +  L+L  P  +   +  D D D +   SP S++CCR++A   
Sbjct: 121 FHYTGIS--MNFGSDWGIEGLDLRNPYFLTWGD-ADDDHDLYSFHSPTSLICCRLIA--- 180

Query: 181 FPTSSFYFISSFLISFIVLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVR 240
                        + F++LL LRH+LP+++ G  ++S+TLLML ++R +GILL  YV  +
Sbjct: 181 -------------LLFVLLLFLRHSLPVLLGGVDDFSITLLMLPLVRTLGILLIAYVFFK 238

Query: 241 AFTSIQRRRHYQDPRLRLATPEDE 264
           +F  IQR R  +D RL   + ++E
Sbjct: 241 SFIVIQRCRQERDTRLSGFSSDEE 238

BLAST of Cp4.1LG03g05790 vs. TAIR 10
Match: AT2G02960.2 (RING/FYVE/PHD zinc finger superfamily protein )

HSP 1 Score: 147.5 bits (371), Expect = 1.6e-35
Identity = 83/200 (41.50%), Postives = 111/200 (55.50%), Query Frame = 0

Query: 59  QCRICHDEDDESKMETPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLP 118
           +CRIC DE     +E+PC+C GSLKYAHRKCVQRWCNEKGN ICEICHQ ++PGYTAP P
Sbjct: 42  ECRICSDESPVENLESPCACSGSLKYAHRKCVQRWCNEKGNIICEICHQPYQPGYTAPPP 101

Query: 119 VLYYGDITSPVHFRESWEMSRLNLHVP----AGMPDHEYPDSDFDEFFTPSPRSILCCRM 178
            L   + T  +     W +S L++H P        +  Y +S++ E+   S      CR 
Sbjct: 102 PLQPEETT--IDIGGGWTISGLDVHDPRLLAIAEAERRYLESEYVEYTASSASGAAFCRS 161

Query: 179 VAVTVFPTSSFYFISSFLISFIVLLVLRHTLPIVISGAGEYS--LTLLMLLILRIVGILL 238
            A+ +                + LL+LRH L I     GE     ++L L++LR  G LL
Sbjct: 162 AALIL----------------MALLLLRHALTITDDTDGEEDDPSSILSLVLLRAAGFLL 221

Query: 239 PIYVMVRAFTSIQRRRHYQD 253
           P Y+M  A + +QRRR  Q+
Sbjct: 222 PCYIMAWAISILQRRRQRQE 223

BLAST of Cp4.1LG03g05790 vs. TAIR 10
Match: AT2G02960.4 (RING/FYVE/PHD zinc finger superfamily protein )

HSP 1 Score: 147.5 bits (371), Expect = 1.6e-35
Identity = 83/200 (41.50%), Postives = 111/200 (55.50%), Query Frame = 0

Query: 59  QCRICHDEDDESKMETPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLP 118
           +CRIC DE     +E+PC+C GSLKYAHRKCVQRWCNEKGN ICEICHQ ++PGYTAP P
Sbjct: 42  ECRICSDESPVENLESPCACSGSLKYAHRKCVQRWCNEKGNIICEICHQPYQPGYTAPPP 101

Query: 119 VLYYGDITSPVHFRESWEMSRLNLHVP----AGMPDHEYPDSDFDEFFTPSPRSILCCRM 178
            L   + T  +     W +S L++H P        +  Y +S++ E+   S      CR 
Sbjct: 102 PLQPEETT--IDIGGGWTISGLDVHDPRLLAIAEAERRYLESEYVEYTASSASGAAFCRS 161

Query: 179 VAVTVFPTSSFYFISSFLISFIVLLVLRHTLPIVISGAGEYS--LTLLMLLILRIVGILL 238
            A+ +                + LL+LRH L I     GE     ++L L++LR  G LL
Sbjct: 162 AALIL----------------MALLLLRHALTITDDTDGEEDDPSSILSLVLLRAAGFLL 221

Query: 239 PIYVMVRAFTSIQRRRHYQD 253
           P Y+M  A + +QRRR  Q+
Sbjct: 222 PCYIMAWAISILQRRRQRQE 223

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5T0T03.2e-0946.67E3 ubiquitin-protein ligase MARCHF8 OS=Homo sapiens OX=9606 GN=MARCHF8 PE=1 SV=1[more]
Q5XH395.5e-0946.67E3 ubiquitin-protein ligase MARCHF8 OS=Xenopus laevis OX=8355 GN=marchf8 PE=2 SV... [more]
Q0VD597.2e-0945.00E3 ubiquitin-protein ligase MARCHF8 OS=Bos taurus OX=9913 GN=MARCHF8 PE=2 SV=1[more]
Q9DBD27.2e-0945.00E3 ubiquitin-protein ligase MARCHF8 OS=Mus musculus OX=10090 GN=Marchf8 PE=2 SV=... [more]
Q28IK89.4e-0936.00E3 ubiquitin-protein ligase MARCHF8 OS=Xenopus tropicalis OX=8364 GN=marchf8 PE=... [more]
Match NameE-valueIdentityDescription
XP_023528859.12.35e-18794.24uncharacterized protein LOC111791660 isoform X1 [Cucurbita pepo subsp. pepo] >XP... [more]
KAG7017957.11.11e-18493.88E3 ubiquitin-protein ligase MARCH8, partial [Cucurbita argyrosperma subsp. argyr... [more]
XP_022984108.11.30e-18493.17uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984109... [more]
XP_023528862.13.22e-18492.81uncharacterized protein LOC111791660 isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_022935629.12.16e-18392.45uncharacterized protein LOC111442434 isoform X1 [Cucurbita moschata] >XP_0229356... [more]
Match NameE-valueIdentityDescription
A0A6J1J9L96.30e-18593.17uncharacterized protein LOC111482515 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1FB671.04e-18392.45uncharacterized protein LOC111442434 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1J9N68.65e-18291.73uncharacterized protein LOC111482515 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1F6551.43e-18091.01uncharacterized protein LOC111442434 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A0A0LBM82.95e-14977.86RING-CH-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G18672... [more]
Match NameE-valueIdentityDescription
AT2G01275.14.9e-5342.31RING/FYVE/PHD zinc finger superfamily protein [more]
AT2G01275.24.9e-5342.31RING/FYVE/PHD zinc finger superfamily protein [more]
AT5G38070.16.0e-5141.67RING/FYVE/PHD zinc finger superfamily protein [more]
AT2G02960.21.6e-3541.50RING/FYVE/PHD zinc finger superfamily protein [more]
AT2G02960.41.6e-3541.50RING/FYVE/PHD zinc finger superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011016Zinc finger, RING-CH-typeSMARTSM00744ringv_2coord: 59..106
e-value: 2.6E-20
score: 83.5
IPR011016Zinc finger, RING-CH-typePFAMPF12906RINGvcoord: 60..105
e-value: 4.4E-12
score: 45.9
IPR011016Zinc finger, RING-CH-typePROSITEPS51292ZF_RING_CHcoord: 52..112
score: 21.969488
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 48..114
e-value: 1.5E-19
score: 71.4
IPR022143Protein of unknown function DUF3675PFAMPF12428DUF3675coord: 111..240
e-value: 2.7E-28
score: 98.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 255..278
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 255..269
NoneNo IPR availablePANTHERPTHR23012:SF174E3 UBIQUITIN-PROTEIN LIGASE MARCH-RELATEDcoord: 1..271
NoneNo IPR availableCDDcd16495RING_CH-C4HC3_MARCHcoord: 60..106
e-value: 7.37376E-19
score: 76.212
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 57..109
IPR033275E3 ubiquitin-protein ligase MARCH-likePANTHERPTHR23012RING/FYVE/PHD ZINC FINGER DOMAIN-CONTAININGcoord: 1..271

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG03g05790.1Cp4.1LG03g05790.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004842 ubiquitin-protein transferase activity
molecular_function GO:0008270 zinc ion binding