Cp4.1LG01g16340 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG01g16340
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionpeptide chain release factor 1
LocationCp4.1LG01: 10089331 .. 10095709 (+)
RNA-Seq ExpressionCp4.1LG01g16340
SyntenyCp4.1LG01g16340
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAACCAACACAGCCTCTTCTCGCCATTGCTTACTGCTCATTTTCAAATGCTAAGGCAAAAACGACCGGCGGAAACCAAACGGCCGGCGGGTACCAGGAGACAACCCAAGCAAGATTCCGGCGAATAACGGAGAATGGGAGGGTCCCACTCCCACCCACCGTTATCGGTCCACACTACTAGGCTCCGGTTCGGTTTGGCCCAAAACCTAATGGTCTAACCACGCCCGATTTCAGACAGCCGGACCGGAGATCCTCATCGTTCATCTCTTTGCTCAAGTTTACCCTTCCGCCCTGTGCTCTAACATCAGACCTTCTGGTCTGCTGCATCCATCGCAACGCCCCCCCATACTACAGATTAGATCTAGAAGAACACTATGAGAATCCATCGACCAACTGTGCGGCTTCTTGAACAAATCCGAACACTTGCCTTACATTGCCGCACGAATCCTGCGCAAATCTGTACAAATCCGTTGCTTCCGCCACCGTCCGACCTTCTTTCTACTTCGCCTTTTCGTTTTTATTCCAAAGGTTCTTATCTTTCTTCCCTTTTTTCAATCCTTTTCTTTGTTTTCTTCGTTATCACGGCTTTGGCTCAGGCACGGATTTATTGGGTTAGGTCTCTTCCACATTTTACATTGGATGCTGTGGATTACTTGTTCATTTGAATTATGAAATGTAGAATTGAGTTCTGTATTATTTATTGGCTATTACCTTTTGATTGAAATCTTTGTTTGCATCTTTCGATGCTTGGCCTCCTATAATCTGAACATCTCTTCAAACTTGTTTAAAATAGGCGAAGTACAATCGCAGCAGCTATCAATGGAGCTTATCAAGATAATGGAACAAAGGTTGAAGGCCATTGAGCTCAGGAGCTCTTTTCTTCTGAATATAATCAACCGGGTACCACTTATTCTCACTTTTTTATGCTCTCTCCTTTGCTGTACAGACATCACACAGCTTCAAAATTTTGTTTAAGTTAACATATTAAAAGTTGTACAACAAATCGAATGAGATAAAATATGATCTTACGAAACTTCATAATATCCATATTCTACCTTAGCCTTCCCGCCTAGAAGCTGTAATTCTCACACATTTGGAAGATAAATAGGTTTGGAGATTAGAAAGCAGTGACACATTCACAACCAATTCTTTCCTCCTCGACTAAAACATCATTGCTGAAGAGAAACGACCCTTTTACTATCGTATTCAATTTGGATAGACTCATATTCTGTAACGGCCCAAGCCCACCGCTAACAAATATCGTCTCTTTGAGCTTTTCCTTTCGGGTAGTTTCGTTCTCCTCCCCATCCAATGTGGGATCTCACAATCCACCCCCTTCAGCGCCCAATGTCCTCACTGGCACTCTTTCCATTCTTCAATCGACGTGGGATCCCCCAATCCACCCCCTTACTAGCACAATGTTCTTACTAGCACACCGCCTCGTGTCCACCTTCCTTCAGGGCTCAGACTCCTCACTGGCACATTGTCGAGTGTTTGGCTTGGATACCATTTGTAACAGTCCAAGCCTACCGCTAACAGATATTGTCCTCTTTGAGCTTTCCCTTTTGGACTTCCTCTCAAGGTTTTTAAAACATGTCTGCTAGGGAGAGGTTTCCACACCCATATAAATGATGTTTTGTTCTCCTCTCCAACCGATGTGGGATCTTAACTATTCAAAGAAGATAAAATTCTTTCTTTGGAAGCTTGCTCATAATGTTGCAATACCCATGACCTCTTACAGCAGCCTATGCCATATTTATCAATCTCGCTTCCATGCAAGAATGAAGCTGAATCTCAGTCACAAGGCTTGCTGTCTCGCTCTTTGGAATGTATGCTCTCTTCCTTTGGTTGGTCACTGGCTTTACGACGTAATCCCAAGGGTTTCCTCTCATTCCTCTCCCAGAACACCCCTTCTGAAAGGAGAAGAAAACCCTTTAGATAAACATAATTAGAGCTTTCTTTTGGGCAATTTGGATTTAAAGGAACCATTGGCTTCTTTCCGAGAAAGAACCTTACAGCTGGATTCTTCAGAAAATAATTGTCTTAGCTATCTCGTTGTATAAATTTTCTTTCTTCTTAATTCTTGTATTTTTGTTTCTCTTTGTTCAGCAAATATTTTCTTATCTAAAAGAAAAGTAATCAAATAAATAAAGAAAATTTTAGCTTTCCCACCCTTCCTACTCTTCCTTTTCTCCTGTATCCCTTTCTCTTATAATTACAGACCTTAAATGATGATTTAATTCTCTTTACTCATGGTGTTATTTGAACATAGCCAGAAGCCCCACCGTCCGAGTATGCAAAGGCTAACAAGGAGCTTAAGAAACTCAGGGATACGATGGATCTAATAAATGAGTTGAGGTCTAGACAGAAGGTATGAAATACATTCAACACATTGTCTTTCTTTACAATACGAACTGCTTAATCTGTTATCTATGAAAAGGTTAAGAGATGACTGGATTATTTCCTTTCAGTGGAAAGACGACCTCTGTTCATGCTGTTCTTTCACAATCATCTACTAATTTGATATTCATTTGGCTTGGTTGTTGTCCAAACTCCAAAGTGTTCTAAATAAAACTTGTGTGGGTGTTTGTGTTTTACAATGAGATTGTAGACGCCTATTGAAATTCTCTAAGTGGACTGACTTGGAGGCATTTGCTTCCCCAATGAATGAAATGTGTGTGGTTGCCGTACTATTTACAGGAAGGATATCAGAAAATTTTTATGACTGTTTTGGCTGTAGGAAATTAATGGTTTGAGAACGCTGATGGACGAATGCCCAGAGGATAAAGATATGCTTGATATGGCTACCGAGGAATTAAATCAGGCAGTTGAAGAAGAGAAAAGATTACAGCACTCATTGCTCACGTCGTTACTTCCAAGAGATGATGCTGATGAGAGGGGTTGCATCTTGGAGGTGAGAGCAGGTAGGTAATGGTGCATATTAGTGAACGTAGCCATTAATGGGAGTCTTTTTTTGTTGTGAATAGGAATTTTTCTGTTCTCTTTTTCTCCGTTTTCGATCTCGACAGTTAAAACTAATAGAAAGTTTTACCAGGAACTGGTGGAGAAGAAGCTTCTTTGTTTGCCATGGATATATTCAAAATGTGAGTGACAGTTGCATAGCATGCTTAGTATCACTGATTATAATAATTCCACACGTTCTAGTTCTCAAGAGTTATTTACTTTGGATCAAAATAGGTATGAGCGATACGCACAGAAAAAGGGTTGGAAGTTTGAGGTGGTTGACATAACAGAATCAGATCTTAAAGGATATAAGGTGATTTCATTATCAAATTTTTTAGTTCAACAATGGGGTAATAAACCTAGAGTACATGAATATAAATTTAGGATTTATCGTCCTACATTGTAGGAAGCGGTTGCTGCAATATCAGGAGCCGGTGTGTATGGGAAACTTAAATTTGAGAGTGGCATACACCGAGTACAGGTCTTCTCGACATGCATATCAATAGCCTTTAGCATTATTTACCATTGTGAAATGATAATTGAAACCATTCTCTTCCTTGGGCAGCGAGTTCCTGTGACAGAGAAGTCTGGGCGTGTTCATACCAGTGCTGTTTCTGTAGCCATTCTCCCTCAAGCAGACGAGGTTCCAACCCTTCTCGCTTGACGCTTTGAAGCCACTTTCTTCCTAGAAAATTAATATCCATAGTAATTTAGGCAAGGGATTGATTCTAAGTTTGGTTGTGATATTGTTGAGGGAATGTGCAGGTAGATGTAGAGTTGAAGAACGAGGACCTGAGAATAGATACATTCAGATCAGGCGGTTCAGGAGGTCAGCATGCAAATACCACAAATAGTGCCGTTAGAGTGACTCACATTCCAACTGGGTTGACTGTGTCCATACAAGACGAGCGATCCCAACATATGGTAGGAACAGTTTCGTTTCTATTGCTCGTATGATTTTGATGGGGTATTTGTGTTTGGTTTAACTTGTTTGAGTGTGCTTTCTTTGGTTCAAACAGAACAAGGCGAAGGCGCTTAAAGTTATATGTGCAAAGCTATACGAAATTGAAAGGCGCAGGATTCAGAGCAGTCGGTCAAAGCTCCGATCGGAACAGGTAATAAGAAAGAGATTGATGTATGATGTATATGGTTACTGTGTGTTCAAAGAAAATACTAAAGGAAGTGAGTTTGGATTATTAAAAGCAGATTGGTAGCGGGGACAGATCGGAACGCATTCGCACATACAACTTCCCGCAAGGGCGGGTAACAGACCATCGGGTAGGCATTACAGAGCATGCCATTGAGGAGGTGATGCAGGGACAGAATCTGGATATGTTCGTGGACGCTCTGTTATTGCAGCAGGAAATGGATGCAATTGCATCCTTCACTTCCTCTTCTTCGTAGTGGCAGTGGTAGTGAAGAGGAGGAGCATTGCATCAAATAGGATCTGCCCTCTTCGCATTAGATTCAGGTAACATAAGAAATGTGTTTCTTATAGAAGTACAATACGAGTAGAGTGGGTGGTTACAATACAACTTTAAAGGGATTAAATTTCATATATGTTACCATGATTAGTAGTATGTAGGAGTTCGTTTTTGTTTGATACAACATAATTGTAACATCCCAGGCCCATCGCTAGTAGATATTGTCTTATTTGGGTTTTCCCTTCCATAAATCATACTTCCCAAAATTCAGAGTCTTACAATATGTAACCATCTAAGCCCCACCGCTATCGGATATTGAGCTTTCCTTTCCAGGCTTCCCTTCAAGGTTTTAAGACCCGTCCACTAGGGAGAGGTTTCCACACCCTTAAAAGAAATGTTTTATTCCCCTCTCCAACTGATGTGGGATCTCACAATCCACTCCCTTGGGGGCCAAGCATCCTCGTTGGTACACCGCCCGGTGTCTGGCTCTAATATCATTTGTAACAACCTAAGCTTGCTGCTAGCAGATATTATTTTCTTTGGGCTTTCCCTTCCGAGCTTCCCTTCAAGGTTTTAAAACGTATTCACTAGGGAGAGGTCTTCACACCTTTAAAAGGAATGTTTTGTTCCCCTCTCCAACTGATGTGGGATCTTACCATCCACTCCCTTGGGGACCCACCGTCCTCGCTAGTACACCGCTCGGTGTCTGATTCTGATACTATTTGTAACCACCTAATTTCACCGCTAGTAGATATTATTCTCTTTAGGCTTCCTCTCACGGTTTTAAAACGCGTTCATTAGAGAGAGGTTTTCCCACCCTTAAAATGAAGGCTTTGTTCCCTCTCCAATCAATGTGAGATCTCACACAAGAATACAACAGTGTCATTAAAAAAGTCGTCCTGATCCGGGAAAAGGAAGAATCTCGATGAAAGTTAAAACAAATAATAAAAGGCAAAGGAAAAGAGAAGGCAAAGCAAAGGTAGAAGGGGATGGAATATGAGGGCAGCCAGGGAAAAGACAGTGCATGAGTAGAGGCACAACAGGAAAAGGATAAGGCAAAGGCTAAGTTGATTTCAATCTGTTGAGAGCTCCATCCATGCTCTACCTGTTCCCTGCTGCCCTTATCCTCAATCCCCAAAACTGACAGATTCACATGGATGCCATGGCCATTACAACAGTACAGTGGTGGAGTGTGCTGGTCTGGTGTGTTTGTGAAGGATGCCATGGCCACCTGTATTCGACTGTTTTTGAAAATTATGTTTCTTTCGACGATTTCGATTCCCACAGTCCCAAATCTCTGTACTCTTTTGGATTTTCCACTTTTCACCAACAACTCCATTCTCCATTCCGTTTTGCTTCTGTTTTCCACTCCTCACCAACATCTCTCCATTAAACAAAAAACAATTCACAGAAATTTTCCACTTTCAGTGTTACTCATAGTTTCTTAAAATGGATACAAAAATGATTTGGGTTATGGACCCTACAATTATCCCACTGTTTCTTCCTCTATTTGTTGCAGTAGCTGTTTGTTAATCACAAAAATTGGTTCTCTCTCCATTGAATCAACCATGGGCAACCGATCTTCTCATAAGAGGTAGATGAGGCAGTCGATGGCAGGATTTGTGTAATAGTCCAAGCTCATTACTAGCCGATATTGTTCGCTTTGGCACGCTATGCATCGCCGTCAGCTTCATGATTTTAAAACACGTTTGCTAGGAAGAAATTTTTACATACTTATAAGGAATGCTTCTGTGAAATCATATATCGATTGGAGAGAGGAACAAGTACCATGGATTGTGAGATCCCACATTGGTTAGGGAGGAGAACGAAACATGTGTGAAAACTTCTCCTTATCATACAAAAAGAGAGATATCCCGGACTTGGAAAGGGCCTTGGCGATACACATCTGGTGTTTGCTTAATGGATAGAATAGATAGTGGTTTGTAGACCCAATATCATGGTGGAGCTGGTGCTTGGCCTTTTATCCAACGC

mRNA sequence

GAACCAACACAGCCTCTTCTCGCCATTGCTTACTGCTCATTTTCAAATGCTAAGGCAAAAACGACCGGCGGAAACCAAACGGCCGGCGGGTACCAGGAGACAACCCAAGCAAGATTCCGGCGAATAACGGAGAATGGGAGGGTCCCACTCCCACCCACCGTTATCGGTCCACACTACTAGGCTCCGGTTCGGTTTGGCCCAAAACCTAATGGTCTAACCACGCCCGATTTCAGACAGCCGGACCGGAGATCCTCATCGTTCATCTCTTTGCTCAAGTTTACCCTTCCGCCCTGTGCTCTAACATCAGACCTTCTGGTCTGCTGCATCCATCGCAACGCCCCCCCATACTACAGATTAGATCTAGAAGAACACTATGAGAATCCATCGACCAACTGTGCGGCTTCTTGAACAAATCCGAACACTTGCCTTACATTGCCGCACGAATCCTGCGCAAATCTGTACAAATCCGTTGCTTCCGCCACCGTCCGACCTTCTTTCTACTTCGCCTTTTCGTTTTTATTCCAAAGGCGAAGTACAATCGCAGCAGCTATCAATGGAGCTTATCAAGATAATGGAACAAAGGTTGAAGGCCATTGAGCTCAGGAGCTCTTTTCTTCTGAATATAATCAACCGGCCAGAAGCCCCACCGTCCGAGTATGCAAAGGCTAACAAGGAGCTTAAGAAACTCAGGGATACGATGGATCTAATAAATGAGTTGAGGTCTAGACAGAAGGAAATTAATGGTTTGAGAACGCTGATGGACGAATGCCCAGAGGATAAAGATATGCTTGATATGGCTACCGAGGAATTAAATCAGGCAGTTGAAGAAGAGAAAAGATTACAGCACTCATTGCTCACGTCGTTACTTCCAAGAGATGATGCTGATGAGAGGGGTTGCATCTTGGAGGTGAGAGCAGGAACTGGTGGAGAAGAAGCTTCTTTGTTTGCCATGGATATATTCAAAATGTATGAGCGATACGCACAGAAAAAGGGTTGGAAGTTTGAGGTGGTTGACATAACAGAATCAGATCTTAAAGGATATAAGGAAGCGGTTGCTGCAATATCAGGAGCCGGTGTGTATGGGAAACTTAAATTTGAGAGTGGCATACACCGAGTACAGCGAGTTCCTGTGACAGAGAAGTCTGGGCGTGTTCATACCAGTGCTGTTTCTGTAGCCATTCTCCCTCAAGCAGACGAGGTAGATGTAGAGTTGAAGAACGAGGACCTGAGAATAGATACATTCAGATCAGGCGGTTCAGGAGGTCAGCATGCAAATACCACAAATAGTGCCGTTAGAGTGACTCACATTCCAACTGGGTTGACTGTGTCCATACAAGACGAGCGATCCCAACATATGAACAAGGCGAAGGCGCTTAAAGTTATATGTGCAAAGCTATACGAAATTGAAAGGCGCAGGATTCAGAGCAGTCGGTCAAAGCTCCGATCGGAACAGATTGGTAGCGGGGACAGATCGGAACGCATTCGCACATACAACTTCCCGCAAGGGCGGGTAACAGACCATCGGGTAGGCATTACAGAGCATGCCATTGAGGAGGTGATGCAGGGACAGAATCTGGATATGTTCGTGGACGCTCTGTTATTGCAGCAGGAAATGGATGCAATTGCATCCTTCACTTCCTCTTCTTCGTAGTGGCAGTGGTAGTGAAGAGGAGGAGCATTGCATCAAATAGGATCTGCCCTCTTCGCATTAGATTCAGTAGCTGTTTGTTAATCACAAAAATTGGTTCTCTCTCCATTGAATCAACCATGGGCAACCGATCTTCTCATAAGAGGTAGATGAGGCAGTCGATGGCAGGATTTGTGTAATAGTCCAAGCTCATTACTAGCCGATATTGTTCGCTTTGGCACGCTATGCATCGCCGTCAGCTTCATGATTTTAAAACACGTTTGCTAGGAAGAAATTTTTACATACTTATAAGGAATGCTTCTGTGAAATCATATATCGATTGGAGAGAGGAACAAGTACCATGGATTGTGAGATCCCACATTGGTTAGGGAGGAGAACGAAACATGTGTGAAAACTTCTCCTTATCATACAAAAAGAGAGATATCCCGGACTTGGAAAGGGCCTTGGCGATACACATCTGGTGTTTGCTTAATGGATAGAATAGATAGTGGTTTGTAGACCCAATATCATGGTGGAGCTGGTGCTTGGCCTTTTATCCAACGC

Coding sequence (CDS)

ATGAGAATCCATCGACCAACTGTGCGGCTTCTTGAACAAATCCGAACACTTGCCTTACATTGCCGCACGAATCCTGCGCAAATCTGTACAAATCCGTTGCTTCCGCCACCGTCCGACCTTCTTTCTACTTCGCCTTTTCGTTTTTATTCCAAAGGCGAAGTACAATCGCAGCAGCTATCAATGGAGCTTATCAAGATAATGGAACAAAGGTTGAAGGCCATTGAGCTCAGGAGCTCTTTTCTTCTGAATATAATCAACCGGCCAGAAGCCCCACCGTCCGAGTATGCAAAGGCTAACAAGGAGCTTAAGAAACTCAGGGATACGATGGATCTAATAAATGAGTTGAGGTCTAGACAGAAGGAAATTAATGGTTTGAGAACGCTGATGGACGAATGCCCAGAGGATAAAGATATGCTTGATATGGCTACCGAGGAATTAAATCAGGCAGTTGAAGAAGAGAAAAGATTACAGCACTCATTGCTCACGTCGTTACTTCCAAGAGATGATGCTGATGAGAGGGGTTGCATCTTGGAGGTGAGAGCAGGAACTGGTGGAGAAGAAGCTTCTTTGTTTGCCATGGATATATTCAAAATGTATGAGCGATACGCACAGAAAAAGGGTTGGAAGTTTGAGGTGGTTGACATAACAGAATCAGATCTTAAAGGATATAAGGAAGCGGTTGCTGCAATATCAGGAGCCGGTGTGTATGGGAAACTTAAATTTGAGAGTGGCATACACCGAGTACAGCGAGTTCCTGTGACAGAGAAGTCTGGGCGTGTTCATACCAGTGCTGTTTCTGTAGCCATTCTCCCTCAAGCAGACGAGGTAGATGTAGAGTTGAAGAACGAGGACCTGAGAATAGATACATTCAGATCAGGCGGTTCAGGAGGTCAGCATGCAAATACCACAAATAGTGCCGTTAGAGTGACTCACATTCCAACTGGGTTGACTGTGTCCATACAAGACGAGCGATCCCAACATATGAACAAGGCGAAGGCGCTTAAAGTTATATGTGCAAAGCTATACGAAATTGAAAGGCGCAGGATTCAGAGCAGTCGGTCAAAGCTCCGATCGGAACAGATTGGTAGCGGGGACAGATCGGAACGCATTCGCACATACAACTTCCCGCAAGGGCGGGTAACAGACCATCGGGTAGGCATTACAGAGCATGCCATTGAGGAGGTGATGCAGGGACAGAATCTGGATATGTTCGTGGACGCTCTGTTATTGCAGCAGGAAATGGATGCAATTGCATCCTTCACTTCCTCTTCTTCGTAG

Protein sequence

MRIHRPTVRLLEQIRTLALHCRTNPAQICTNPLLPPPSDLLSTSPFRFYSKGEVQSQQLSMELIKIMEQRLKAIELRSSFLLNIINRPEAPPSEYAKANKELKKLRDTMDLINELRSRQKEINGLRTLMDECPEDKDMLDMATEELNQAVEEEKRLQHSLLTSLLPRDDADERGCILEVRAGTGGEEASLFAMDIFKMYERYAQKKGWKFEVVDITESDLKGYKEAVAAISGAGVYGKLKFESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHANTTNSAVRVTHIPTGLTVSIQDERSQHMNKAKALKVICAKLYEIERRRIQSSRSKLRSEQIGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASFTSSSS
Homology
BLAST of Cp4.1LG01g16340 vs. ExPASy Swiss-Prot
Match: A2RVR7 (Peptide chain release factor 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At2g47020 PE=2 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 2.7e-164
Identity = 295/385 (76.62%), Postives = 349/385 (90.65%), Query Frame = 0

Query: 40  LLSTSPFRFYSKGEVQSQQLSMELIKIMEQRLKAIELRSSFLLNIINRPEAPPSEYAKAN 99
           +LS++  R Y+ G     QLS +LIKIM+QRL AIE R++ L  +IN+PE  P E+++AN
Sbjct: 31  VLSSNMIRLYTTG--MEPQLSPDLIKIMDQRLSAIEHRNAVLQKLINQPEYSPEEFSRAN 90

Query: 100 KELKKLRDTMDLINELRSRQKEINGLRTLMDECPEDKDMLDMATEELNQAVEEEKRLQHS 159
           KEL+KLRD+M LIN+LR++QKEI+GL++L+ E  +DKDMLD+A  EL++AVEEEKRLQ  
Sbjct: 91  KELRKLRDSMLLINDLRAKQKEIDGLKSLVSESSDDKDMLDLAVGELDEAVEEEKRLQTL 150

Query: 160 LLTSLLPRDDADERGCILEVRAGTGGEEASLFAMDIFKMYERYAQKKGWKFEVVDITESD 219
           LL SLLP+D+ADER CILEVRAGTGGEEASLFAMDIF+MYERY+QKKGWKF++VDITESD
Sbjct: 151 LLKSLLPKDEADERDCILEVRAGTGGEEASLFAMDIFRMYERYSQKKGWKFDIVDITESD 210

Query: 220 LKGYKEAVAAISGAGVYGKLKFESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVE 279
           +KGYKEA AAI GA VYGKLKFESGIHRVQR+P+TEKSGR+HTSA+SVAILPQADEVDV+
Sbjct: 211 MKGYKEASAAICGASVYGKLKFESGIHRVQRIPITEKSGRIHTSAISVAILPQADEVDVQ 270

Query: 280 LKNEDLRIDTFRSGGSGGQHANTTNSAVRVTHIPTGLTVSIQDERSQHMNKAKALKVICA 339
           L+NEDLRIDT+RSGGSGGQHANTTNSAVR+ H+PTG+ VSIQDERSQHMN+AKALKV+CA
Sbjct: 271 LRNEDLRIDTYRSGGSGGQHANTTNSAVRIIHLPTGMMVSIQDERSQHMNRAKALKVLCA 330

Query: 340 KLYEIERRRIQSSRSKLRSEQIGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQ 399
           +LYEIER RIQSSRSKLRS+QIGSGDRS RIRTYNFPQGRVTDHRVGIT HAIE++MQG+
Sbjct: 331 RLYEIERLRIQSSRSKLRSDQIGSGDRSGRIRTYNFPQGRVTDHRVGITHHAIEDMMQGE 390

Query: 400 NLDMFVDALLLQQEMDAIASFTSSS 425
           NLDMF+DALLL+QEMDAIASF+S+S
Sbjct: 391 NLDMFIDALLLRQEMDAIASFSSTS 413

BLAST of Cp4.1LG01g16340 vs. ExPASy Swiss-Prot
Match: Q2RWE1 (Peptide chain release factor 1 OS=Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIMB 8255 / S1) OX=269796 GN=prfA PE=3 SV=1)

HSP 1 Score: 358.6 bits (919), Expect = 9.7e-98
Identity = 180/352 (51.14%), Postives = 254/352 (72.16%), Query Frame = 0

Query: 67  MEQRLKAIELRSSFLLNIINRPEAPPSEYAKANKELKKLRDTMDLINELRSRQKEINGLR 126
           +E+ L  +  R   L  +++        + + ++EL  L   ++ I  L   + E+ G R
Sbjct: 3   LEENLSRVVARHDELRALLSTSAGGGEGFVRMSRELADLAPVVEAIGRLDDAKAELAGAR 62

Query: 127 TLMDECPEDKDMLDMATEELNQAVEEEKRLQHSLLTSLLPRDDADERGCILEVRAGTGGE 186
            L+D    D DM D+A EE+ +   E   L+H +   LLPRD+AD+RG ILEVRAGTGG+
Sbjct: 63  ALLD----DPDMRDLAREEVLRLEAEIPALEHGVKLLLLPRDEADDRGVILEVRAGTGGD 122

Query: 187 EASLFAMDIFKMYERYAQKKGWKFEVVDITESDLKGYKEAVAAISGAGVYGKLKFESGIH 246
           EA+LFA D+ +MYERYAQ++GW+FEV++ +++D+ G KEA AAISG GV+ +LKFESG+H
Sbjct: 123 EAALFAGDLLRMYERYAQERGWRFEVMEASDTDIGGIKEASAAISGRGVFARLKFESGVH 182

Query: 247 RVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHANTTNSA 306
           RVQRVPVTE  GR+HTSA +VA+LP+A+EVDVE++ +DLRIDT+RS G+GGQH NTT+SA
Sbjct: 183 RVQRVPVTEGGGRIHTSAATVAVLPEAEEVDVEIEEKDLRIDTYRSQGAGGQHVNTTDSA 242

Query: 307 VRVTHIPTGLTVSIQDERSQHMNKAKALKVICAKLYEIERRRIQSSRSKLRSEQIGSGDR 366
           VR+TH+P+G+    QDE+SQH NKAKA++++  KLY+  R+    +R++ R  Q+GSGDR
Sbjct: 243 VRITHLPSGVVAQCQDEKSQHKNKAKAMRMLRTKLYDHARQTADDARAEARRVQVGSGDR 302

Query: 367 SERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIA 419
           SERIRTYNFPQGRVTDHR+G+T H + EV+ G  LD  ++ L+ + +   +A
Sbjct: 303 SERIRTYNFPQGRVTDHRIGLTLHRLPEVLAGTALDEVIEPLIAEDQATRMA 350

BLAST of Cp4.1LG01g16340 vs. ExPASy Swiss-Prot
Match: A5V9C3 (Peptide chain release factor 1 OS=Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273) OX=392499 GN=prfA PE=3 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 8.2e-97
Identity = 176/353 (49.86%), Postives = 249/353 (70.54%), Query Frame = 0

Query: 66  IMEQRLKAIELRSSFLLNIINRPEAPPSEYAKANKELKKLRDTMDLINELRSRQKEINGL 125
           I   R+  IE R   L  ++ R + P  ++ + +K+  ++        E+R  + E++ L
Sbjct: 4   ITSARIAQIEARRDELQALMARGDLPSDQFVQLSKDYAEIEPVAQAAGEVRRLRAELDVL 63

Query: 126 RTLMDECPEDKDMLDMATEELNQAVEEEKRLQHSLLTSLLPRDDADERGCILEVRAGTGG 185
           + + ++   D ++  MA EE  +        + +L   LLPRD ADER  +LE+RAGTGG
Sbjct: 64  KGMAEDEAGDAELRAMAIEEAEEIRARLPEAERALALKLLPRDAADERSAMLEIRAGTGG 123

Query: 186 EEASLFAMDIFKMYERYAQKKGWKFEVVDITESDLKGYKEAVAAISGAGVYGKLKFESGI 245
           +EA+LFA D+F+MY+RYA+ +GW+ E++  + +DL GYKE VA+++GAGV+ +LKFESG+
Sbjct: 124 DEAALFAGDLFRMYQRYAEDRGWRVELISASAADLGGYKEVVASVTGAGVFARLKFESGV 183

Query: 246 HRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHANTTNS 305
           HRVQRVPVTE  GR+HTSA +VA+LP+A+EVDV + ++DLRID +RS G GGQ  NTT+S
Sbjct: 184 HRVQRVPVTESGGRIHTSAATVAVLPEAEEVDVHIDDKDLRIDVYRSSGPGGQSVNTTDS 243

Query: 306 AVRVTHIPTGLTVSIQDERSQHMNKAKALKVICAKLYEIERRRIQSSRSKLRSEQIGSGD 365
           AVR+TH+PTGL V  QDE+SQH NKAKALKV+  +LYE+ER R+ S R+  R   +GSGD
Sbjct: 244 AVRITHLPTGLVVIQQDEKSQHKNKAKALKVLRTRLYEMERERLASERAGARKSMVGSGD 303

Query: 366 RSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIA 419
           RSERIRTYNFPQGRVTDHR+ +T H + E+++G  LD  V AL+ + E + +A
Sbjct: 304 RSERIRTYNFPQGRVTDHRINLTLHRLSEILEGPGLDDLVGALIAEDEAERLA 356

BLAST of Cp4.1LG01g16340 vs. ExPASy Swiss-Prot
Match: A7HTY3 (Peptide chain release factor 1 OS=Parvibaculum lavamentivorans (strain DS-1 / DSM 13023 / NCIMB 13966) OX=402881 GN=prfA PE=3 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 3.1e-96
Identity = 177/354 (50.00%), Postives = 254/354 (71.75%), Query Frame = 0

Query: 66  IMEQRLKAIELRSSFLLNIINRPEAPPSEYAKANKELKKLRDTMDLINELRSRQKEINGL 125
           I E+RL+++  R   + + +N  + P   +   +KE  +L      I  L +R++E    
Sbjct: 2   IPEERLQSVIARFEAVQSEMN-SDVPRDRFVALSKEFSELEPVAAAIGHLLARRREYADA 61

Query: 126 RTLMDECPEDKDMLDMATEELNQAVEEEKRLQHSLLTSLLPRDDADERGCILEVRAGTGG 185
             L+      +DM +MA EE+ +  EE  RL+H +   LLP+D AD+R  ILEVRAGTGG
Sbjct: 62  EQLL----RVRDMAEMAKEEIARLNEELPRLEHEVQLMLLPKDAADDRNVILEVRAGTGG 121

Query: 186 EEASLFAMDIFKMYERYAQKKGWKFEVVDITESDLKGYKEAVAAISGAGVYGKLKFESGI 245
           +EA+LFA D+F+MYERYA  +GWK E++  +E ++ GYKE +A ISG GVY KLKFESG+
Sbjct: 122 DEAALFAGDLFRMYERYASNQGWKVELIAASEGEMGGYKEIIAGISGPGVYAKLKFESGV 181

Query: 246 HRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHANTTNS 305
           HRVQRVPVTE  GR+HTSA +VA+LP+A+EVDVE++++DLRID +R+ G+GGQH N T S
Sbjct: 182 HRVQRVPVTEGGGRIHTSAATVAVLPEAEEVDVEIEDKDLRIDVYRASGAGGQHVNKTES 241

Query: 306 AVRVTHIPTGLTVSIQDERSQHMNKAKALKVICAKLYEIERRRIQSSRSKLRSEQIGSGD 365
           AVR+TH+PTG+ V+ QDE+SQH NKA+A++++ A++++ ER R+   RS  R  Q+GSGD
Sbjct: 242 AVRITHLPTGIVVAQQDEKSQHKNKARAMQILRARIFDAERERLDRERSAARKGQVGSGD 301

Query: 366 RSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIAS 420
           RSERIRTYNFPQ RVTDHR+ +T H +++V++G+ L   +DAL+ + +   +A+
Sbjct: 302 RSERIRTYNFPQSRVTDHRINLTLHKLDQVLEGEALGELIDALIAEDQAARLAA 350

BLAST of Cp4.1LG01g16340 vs. ExPASy Swiss-Prot
Match: A8GXI1 (Peptide chain release factor 1 OS=Rickettsia bellii (strain OSU 85-389) OX=391896 GN=prfA PE=3 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 4.1e-96
Identity = 174/320 (54.37%), Postives = 236/320 (73.75%), Query Frame = 0

Query: 94  EYAKANKELKKLRDTMDLINELRSRQKEINGLRTLMDECPEDKDMLDMATEELNQAVEEE 153
           E+ KA+KE   L D +  I E    + E+        E   DK  L+M  EE+    +  
Sbjct: 32  EFVKASKEYADLEDIVQKIKEYNKAKAELEEANNFKQEPSLDKATLEMIEEEIRNLEDSL 91

Query: 154 KRLQHSLLTSLLPRDDADERGCILEVRAGTGGEEASLFAMDIFKMYERYAQKKGWKFEVV 213
             L+ S+  +LLP+D+AD +  I+E+RAGTGGEEA+LFA  +F MY+RYA+ KGW+FE++
Sbjct: 92  PTLERSVKIALLPKDEADSKSAIIEIRAGTGGEEAALFAAKLFNMYQRYAELKGWRFEIL 151

Query: 214 DITESDLKGYKEAVAAISGAGVYGKLKFESGIHRVQRVPVTEKSGRVHTSAVSVAILPQA 273
            I E+ + GYKE  A+I G  V+ KLKFESG+HRVQRVP TE  GR+HTSA +VA+LP+ 
Sbjct: 152 SIDETGIGGYKEVSASIKGKDVFSKLKFESGVHRVQRVPETESQGRIHTSAATVAVLPEV 211

Query: 274 DEVDVELKNEDLRIDTFRSGGSGGQHANTTNSAVRVTHIPTGLTVSIQDERSQHMNKAKA 333
           ++VD++L+ +DLRIDT+R+ G+GGQH NTT+SAVR+THIPTG+TV++QDE+SQH NKAKA
Sbjct: 212 EDVDIKLEEKDLRIDTYRASGAGGQHVNTTDSAVRITHIPTGITVALQDEKSQHKNKAKA 271

Query: 334 LKVICAKLYEIERRRIQSSRSKLRSEQIGSGDRSERIRTYNFPQGRVTDHRVGITEHAIE 393
           LK++ A++YE ERR+    R+  R EQIGSGDRSERIRTYNFPQGRV+DHR+ +T + I+
Sbjct: 272 LKILRARIYEEERRKKDQERANNRREQIGSGDRSERIRTYNFPQGRVSDHRINLTLYKID 331

Query: 394 EVMQGQNLDMFVDALLLQQE 414
           EV+ GQ LD F++AL+   E
Sbjct: 332 EVINGQ-LDEFIEALIANDE 350

BLAST of Cp4.1LG01g16340 vs. NCBI nr
Match: XP_023527687.1 (uncharacterized protein LOC111790812 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 816 bits (2108), Expect = 1.14e-297
Identity = 425/425 (100.00%), Postives = 425/425 (100.00%), Query Frame = 0

Query: 1   MRIHRPTVRLLEQIRTLALHCRTNPAQICTNPLLPPPSDLLSTSPFRFYSKGEVQSQQLS 60
           MRIHRPTVRLLEQIRTLALHCRTNPAQICTNPLLPPPSDLLSTSPFRFYSKGEVQSQQLS
Sbjct: 1   MRIHRPTVRLLEQIRTLALHCRTNPAQICTNPLLPPPSDLLSTSPFRFYSKGEVQSQQLS 60

Query: 61  MELIKIMEQRLKAIELRSSFLLNIINRPEAPPSEYAKANKELKKLRDTMDLINELRSRQK 120
           MELIKIMEQRLKAIELRSSFLLNIINRPEAPPSEYAKANKELKKLRDTMDLINELRSRQK
Sbjct: 61  MELIKIMEQRLKAIELRSSFLLNIINRPEAPPSEYAKANKELKKLRDTMDLINELRSRQK 120

Query: 121 EINGLRTLMDECPEDKDMLDMATEELNQAVEEEKRLQHSLLTSLLPRDDADERGCILEVR 180
           EINGLRTLMDECPEDKDMLDMATEELNQAVEEEKRLQHSLLTSLLPRDDADERGCILEVR
Sbjct: 121 EINGLRTLMDECPEDKDMLDMATEELNQAVEEEKRLQHSLLTSLLPRDDADERGCILEVR 180

Query: 181 AGTGGEEASLFAMDIFKMYERYAQKKGWKFEVVDITESDLKGYKEAVAAISGAGVYGKLK 240
           AGTGGEEASLFAMDIFKMYERYAQKKGWKFEVVDITESDLKGYKEAVAAISGAGVYGKLK
Sbjct: 181 AGTGGEEASLFAMDIFKMYERYAQKKGWKFEVVDITESDLKGYKEAVAAISGAGVYGKLK 240

Query: 241 FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA 300
           FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA
Sbjct: 241 FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA 300

Query: 301 NTTNSAVRVTHIPTGLTVSIQDERSQHMNKAKALKVICAKLYEIERRRIQSSRSKLRSEQ 360
           NTTNSAVRVTHIPTGLTVSIQDERSQHMNKAKALKVICAKLYEIERRRIQSSRSKLRSEQ
Sbjct: 301 NTTNSAVRVTHIPTGLTVSIQDERSQHMNKAKALKVICAKLYEIERRRIQSSRSKLRSEQ 360

Query: 361 IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF 420
           IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF
Sbjct: 361 IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF 420

Query: 421 TSSSS 425
           TSSSS
Sbjct: 421 TSSSS 425

BLAST of Cp4.1LG01g16340 vs. NCBI nr
Match: XP_022957138.1 (uncharacterized protein LOC111458610 [Cucurbita moschata] >KAG6601633.1 hypothetical protein SDJN03_06866, partial [Cucurbita argyrosperma subsp. sororia] >KAG7032393.1 prfA [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 805 bits (2079), Expect = 3.00e-293
Identity = 420/425 (98.82%), Postives = 421/425 (99.06%), Query Frame = 0

Query: 1   MRIHRPTVRLLEQIRTLALHCRTNPAQICTNPLLPPPSDLLSTSPFRFYSKGEVQSQQLS 60
           MRIHRPTVRLLEQIRTLALHCRTNPAQ CTNPLLPPPSDLLST   RFYSKGEVQSQQLS
Sbjct: 1   MRIHRPTVRLLEQIRTLALHCRTNPAQTCTNPLLPPPSDLLSTPSVRFYSKGEVQSQQLS 60

Query: 61  MELIKIMEQRLKAIELRSSFLLNIINRPEAPPSEYAKANKELKKLRDTMDLINELRSRQK 120
           MELIKIMEQRLKAIELRSSFLLNIINRPEAPPSEYAKANKELKKLRDTMDLINELRSRQK
Sbjct: 61  MELIKIMEQRLKAIELRSSFLLNIINRPEAPPSEYAKANKELKKLRDTMDLINELRSRQK 120

Query: 121 EINGLRTLMDECPEDKDMLDMATEELNQAVEEEKRLQHSLLTSLLPRDDADERGCILEVR 180
           EINGLRTLMDECPEDKDMLDMATEELNQAVEEEKRLQHSLLTSLLPRDDADERGCILEVR
Sbjct: 121 EINGLRTLMDECPEDKDMLDMATEELNQAVEEEKRLQHSLLTSLLPRDDADERGCILEVR 180

Query: 181 AGTGGEEASLFAMDIFKMYERYAQKKGWKFEVVDITESDLKGYKEAVAAISGAGVYGKLK 240
           AGTGGEEASLFAMDIFKMYERYAQKKGWKFEVVDITESDLKGYKEAVAAISGAGVYGKLK
Sbjct: 181 AGTGGEEASLFAMDIFKMYERYAQKKGWKFEVVDITESDLKGYKEAVAAISGAGVYGKLK 240

Query: 241 FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA 300
           FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA
Sbjct: 241 FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA 300

Query: 301 NTTNSAVRVTHIPTGLTVSIQDERSQHMNKAKALKVICAKLYEIERRRIQSSRSKLRSEQ 360
           NTTNSAVRVTHIP+GLTVSIQDERSQHMNKAKALKVICAKLYEIERRRIQSSRSKLRSEQ
Sbjct: 301 NTTNSAVRVTHIPSGLTVSIQDERSQHMNKAKALKVICAKLYEIERRRIQSSRSKLRSEQ 360

Query: 361 IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF 420
           IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF
Sbjct: 361 IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF 420

Query: 421 TSSSS 425
           TSSSS
Sbjct: 421 TSSSS 425

BLAST of Cp4.1LG01g16340 vs. NCBI nr
Match: XP_022997983.1 (uncharacterized protein LOC111492771 [Cucurbita maxima])

HSP 1 Score: 805 bits (2079), Expect = 3.00e-293
Identity = 419/425 (98.59%), Postives = 422/425 (99.29%), Query Frame = 0

Query: 1   MRIHRPTVRLLEQIRTLALHCRTNPAQICTNPLLPPPSDLLSTSPFRFYSKGEVQSQQLS 60
           MRIHRPTVRLLEQIRTLALHCRTNPAQICTNPLLPPPSDLLST   RFYSKGEVQ+QQLS
Sbjct: 1   MRIHRPTVRLLEQIRTLALHCRTNPAQICTNPLLPPPSDLLSTPSVRFYSKGEVQTQQLS 60

Query: 61  MELIKIMEQRLKAIELRSSFLLNIINRPEAPPSEYAKANKELKKLRDTMDLINELRSRQK 120
           MELIKIMEQRL+AIELRS+FLLNIINRPEAPPSEYAKANKELKKLRDTMDLINELRSRQK
Sbjct: 61  MELIKIMEQRLRAIELRSTFLLNIINRPEAPPSEYAKANKELKKLRDTMDLINELRSRQK 120

Query: 121 EINGLRTLMDECPEDKDMLDMATEELNQAVEEEKRLQHSLLTSLLPRDDADERGCILEVR 180
           EINGLRTLMDECPEDKDMLDMATEELNQAVEEEKRLQHSLLTSLLPRDDADERGCILEVR
Sbjct: 121 EINGLRTLMDECPEDKDMLDMATEELNQAVEEEKRLQHSLLTSLLPRDDADERGCILEVR 180

Query: 181 AGTGGEEASLFAMDIFKMYERYAQKKGWKFEVVDITESDLKGYKEAVAAISGAGVYGKLK 240
           AGTGGEEASLFAMDIFKMYERYAQKKGWKFEVVDITESDLKGYKEAVAAISGAGVYGKLK
Sbjct: 181 AGTGGEEASLFAMDIFKMYERYAQKKGWKFEVVDITESDLKGYKEAVAAISGAGVYGKLK 240

Query: 241 FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA 300
           FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA
Sbjct: 241 FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA 300

Query: 301 NTTNSAVRVTHIPTGLTVSIQDERSQHMNKAKALKVICAKLYEIERRRIQSSRSKLRSEQ 360
           NTTNSAVRVTHIPTGLTVSIQDERSQHMNKAKALKVICAKLYEIERRRIQSSRSKLRSEQ
Sbjct: 301 NTTNSAVRVTHIPTGLTVSIQDERSQHMNKAKALKVICAKLYEIERRRIQSSRSKLRSEQ 360

Query: 361 IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF 420
           IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF
Sbjct: 361 IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF 420

Query: 421 TSSSS 425
           TSSSS
Sbjct: 421 TSSSS 425

BLAST of Cp4.1LG01g16340 vs. NCBI nr
Match: XP_038891510.1 (peptide chain release factor 1, mitochondrial [Benincasa hispida])

HSP 1 Score: 750 bits (1937), Expect = 1.30e-271
Identity = 390/425 (91.76%), Postives = 411/425 (96.71%), Query Frame = 0

Query: 1   MRIHRPTVRLLEQIRTLALHCRTNPAQICTNPLLPPPSDLLSTSPFRFYSKGEVQSQQLS 60
           MRI+R T+RL+EQIRTLALHCRT+P++I TNPLLPPP + LS+  FRFYSKGEVQSQQ S
Sbjct: 1   MRINRGTMRLVEQIRTLALHCRTDPSRISTNPLLPPPFEHLSSPSFRFYSKGEVQSQQPS 60

Query: 61  MELIKIMEQRLKAIELRSSFLLNIINRPEAPPSEYAKANKELKKLRDTMDLINELRSRQK 120
           MEL+KIMEQRL AIELRS+FLLNIIN+PEAPP+EYAKANKELKKLRDTMDLINELRS+QK
Sbjct: 61  MELLKIMEQRLTAIELRSTFLLNIINQPEAPPAEYAKANKELKKLRDTMDLINELRSKQK 120

Query: 121 EINGLRTLMDECPEDKDMLDMATEELNQAVEEEKRLQHSLLTSLLPRDDADERGCILEVR 180
           EINGLRTLM+EC EDKDMLDMATEEL+QAV+EEKRLQ  LL SLLPRDDADER CILEVR
Sbjct: 121 EINGLRTLMEECQEDKDMLDMATEELDQAVQEEKRLQQFLLKSLLPRDDADERDCILEVR 180

Query: 181 AGTGGEEASLFAMDIFKMYERYAQKKGWKFEVVDITESDLKGYKEAVAAISGAGVYGKLK 240
           AGTGGEEASLFAMDIFKMYERYAQKKGWKFEVVDITESDLKGYKEA+AAISGAGVYGKLK
Sbjct: 181 AGTGGEEASLFAMDIFKMYERYAQKKGWKFEVVDITESDLKGYKEAIAAISGAGVYGKLK 240

Query: 241 FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA 300
           FESGIHRVQRVPVTEK+GRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA
Sbjct: 241 FESGIHRVQRVPVTEKAGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA 300

Query: 301 NTTNSAVRVTHIPTGLTVSIQDERSQHMNKAKALKVICAKLYEIERRRIQSSRSKLRSEQ 360
           NTTNSAVRV H+PTGLTVSIQDERSQHMNKAKALKVICAKLYEIERRRIQSSRSKLRSEQ
Sbjct: 301 NTTNSAVRVIHVPTGLTVSIQDERSQHMNKAKALKVICAKLYEIERRRIQSSRSKLRSEQ 360

Query: 361 IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF 420
           IGSGDRSERIRTYNFPQGRVTDHRVGIT+HAIEEVMQGQNLD+FVDALLLQQEMDAIASF
Sbjct: 361 IGSGDRSERIRTYNFPQGRVTDHRVGITDHAIEEVMQGQNLDIFVDALLLQQEMDAIASF 420

Query: 421 TSSSS 425
           TSSSS
Sbjct: 421 TSSSS 425

BLAST of Cp4.1LG01g16340 vs. NCBI nr
Match: XP_022151971.1 (uncharacterized protein LOC111019805 isoform X2 [Momordica charantia])

HSP 1 Score: 733 bits (1893), Expect = 6.30e-265
Identity = 385/424 (90.80%), Postives = 399/424 (94.10%), Query Frame = 0

Query: 1   MRIHRPTVRLLEQIRTLALHCRTNPAQICTNPLLPPPSDLLSTSPFRFYSKGEVQSQQLS 60
           MRI+R T+RL+EQIRTLA H RTNP Q   NP LPPP   LST  FRFYSKGE+Q QQLS
Sbjct: 1   MRINRGTLRLVEQIRTLAPHWRTNPVQNTPNPFLPPPFQPLSTPTFRFYSKGEIQPQQLS 60

Query: 61  MELIKIMEQRLKAIELRSSFLLNIINRPEAPPSEYAKANKELKKLRDTMDLINELRSRQK 120
           MELI IME RLKAIELRS+FLLNIINRPEAPP EYAKANKEL+KLRDTMDLINELRSRQK
Sbjct: 61  MELINIMEHRLKAIELRSTFLLNIINRPEAPPVEYAKANKELRKLRDTMDLINELRSRQK 120

Query: 121 EINGLRTLMDECPEDKDMLDMATEELNQAVEEEKRLQHSLLTSLLPRDDADERGCILEVR 180
           EINGLRTLM ECPEDKDMLDMATEEL+QAVEEEKR+Q+ LL SLLP+DDADER CILEVR
Sbjct: 121 EINGLRTLMTECPEDKDMLDMATEELDQAVEEEKRVQNLLLKSLLPKDDADERDCILEVR 180

Query: 181 AGTGGEEASLFAMDIFKMYERYAQKKGWKFEVVDITESDLKGYKEAVAAISGAGVYGKLK 240
           AGTGGEEASLFAMDIFKMYERY Q+KGWKFEVVDITES+LKGYKEAVAAISGAG YGKLK
Sbjct: 181 AGTGGEEASLFAMDIFKMYERYTQRKGWKFEVVDITESELKGYKEAVAAISGAGAYGKLK 240

Query: 241 FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA 300
           FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA
Sbjct: 241 FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA 300

Query: 301 NTTNSAVRVTHIPTGLTVSIQDERSQHMNKAKALKVICAKLYEIERRRIQSSRSKLRSEQ 360
           NTTNSAVRVTHIPTGL+VSIQDERSQHMNKAKALKVICAKLYE ER RIQSSRSKLRSEQ
Sbjct: 301 NTTNSAVRVTHIPTGLSVSIQDERSQHMNKAKALKVICAKLYEKERCRIQSSRSKLRSEQ 360

Query: 361 IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF 420
           IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF
Sbjct: 361 IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF 420

Query: 421 TSSS 424
           TS+S
Sbjct: 421 TSTS 424

BLAST of Cp4.1LG01g16340 vs. ExPASy TrEMBL
Match: A0A6J1KFI0 (uncharacterized protein LOC111492771 OS=Cucurbita maxima OX=3661 GN=LOC111492771 PE=3 SV=1)

HSP 1 Score: 805 bits (2079), Expect = 1.45e-293
Identity = 419/425 (98.59%), Postives = 422/425 (99.29%), Query Frame = 0

Query: 1   MRIHRPTVRLLEQIRTLALHCRTNPAQICTNPLLPPPSDLLSTSPFRFYSKGEVQSQQLS 60
           MRIHRPTVRLLEQIRTLALHCRTNPAQICTNPLLPPPSDLLST   RFYSKGEVQ+QQLS
Sbjct: 1   MRIHRPTVRLLEQIRTLALHCRTNPAQICTNPLLPPPSDLLSTPSVRFYSKGEVQTQQLS 60

Query: 61  MELIKIMEQRLKAIELRSSFLLNIINRPEAPPSEYAKANKELKKLRDTMDLINELRSRQK 120
           MELIKIMEQRL+AIELRS+FLLNIINRPEAPPSEYAKANKELKKLRDTMDLINELRSRQK
Sbjct: 61  MELIKIMEQRLRAIELRSTFLLNIINRPEAPPSEYAKANKELKKLRDTMDLINELRSRQK 120

Query: 121 EINGLRTLMDECPEDKDMLDMATEELNQAVEEEKRLQHSLLTSLLPRDDADERGCILEVR 180
           EINGLRTLMDECPEDKDMLDMATEELNQAVEEEKRLQHSLLTSLLPRDDADERGCILEVR
Sbjct: 121 EINGLRTLMDECPEDKDMLDMATEELNQAVEEEKRLQHSLLTSLLPRDDADERGCILEVR 180

Query: 181 AGTGGEEASLFAMDIFKMYERYAQKKGWKFEVVDITESDLKGYKEAVAAISGAGVYGKLK 240
           AGTGGEEASLFAMDIFKMYERYAQKKGWKFEVVDITESDLKGYKEAVAAISGAGVYGKLK
Sbjct: 181 AGTGGEEASLFAMDIFKMYERYAQKKGWKFEVVDITESDLKGYKEAVAAISGAGVYGKLK 240

Query: 241 FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA 300
           FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA
Sbjct: 241 FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA 300

Query: 301 NTTNSAVRVTHIPTGLTVSIQDERSQHMNKAKALKVICAKLYEIERRRIQSSRSKLRSEQ 360
           NTTNSAVRVTHIPTGLTVSIQDERSQHMNKAKALKVICAKLYEIERRRIQSSRSKLRSEQ
Sbjct: 301 NTTNSAVRVTHIPTGLTVSIQDERSQHMNKAKALKVICAKLYEIERRRIQSSRSKLRSEQ 360

Query: 361 IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF 420
           IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF
Sbjct: 361 IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF 420

Query: 421 TSSSS 425
           TSSSS
Sbjct: 421 TSSSS 425

BLAST of Cp4.1LG01g16340 vs. ExPASy TrEMBL
Match: A0A6J1GZD2 (uncharacterized protein LOC111458610 OS=Cucurbita moschata OX=3662 GN=LOC111458610 PE=3 SV=1)

HSP 1 Score: 805 bits (2079), Expect = 1.45e-293
Identity = 420/425 (98.82%), Postives = 421/425 (99.06%), Query Frame = 0

Query: 1   MRIHRPTVRLLEQIRTLALHCRTNPAQICTNPLLPPPSDLLSTSPFRFYSKGEVQSQQLS 60
           MRIHRPTVRLLEQIRTLALHCRTNPAQ CTNPLLPPPSDLLST   RFYSKGEVQSQQLS
Sbjct: 1   MRIHRPTVRLLEQIRTLALHCRTNPAQTCTNPLLPPPSDLLSTPSVRFYSKGEVQSQQLS 60

Query: 61  MELIKIMEQRLKAIELRSSFLLNIINRPEAPPSEYAKANKELKKLRDTMDLINELRSRQK 120
           MELIKIMEQRLKAIELRSSFLLNIINRPEAPPSEYAKANKELKKLRDTMDLINELRSRQK
Sbjct: 61  MELIKIMEQRLKAIELRSSFLLNIINRPEAPPSEYAKANKELKKLRDTMDLINELRSRQK 120

Query: 121 EINGLRTLMDECPEDKDMLDMATEELNQAVEEEKRLQHSLLTSLLPRDDADERGCILEVR 180
           EINGLRTLMDECPEDKDMLDMATEELNQAVEEEKRLQHSLLTSLLPRDDADERGCILEVR
Sbjct: 121 EINGLRTLMDECPEDKDMLDMATEELNQAVEEEKRLQHSLLTSLLPRDDADERGCILEVR 180

Query: 181 AGTGGEEASLFAMDIFKMYERYAQKKGWKFEVVDITESDLKGYKEAVAAISGAGVYGKLK 240
           AGTGGEEASLFAMDIFKMYERYAQKKGWKFEVVDITESDLKGYKEAVAAISGAGVYGKLK
Sbjct: 181 AGTGGEEASLFAMDIFKMYERYAQKKGWKFEVVDITESDLKGYKEAVAAISGAGVYGKLK 240

Query: 241 FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA 300
           FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA
Sbjct: 241 FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA 300

Query: 301 NTTNSAVRVTHIPTGLTVSIQDERSQHMNKAKALKVICAKLYEIERRRIQSSRSKLRSEQ 360
           NTTNSAVRVTHIP+GLTVSIQDERSQHMNKAKALKVICAKLYEIERRRIQSSRSKLRSEQ
Sbjct: 301 NTTNSAVRVTHIPSGLTVSIQDERSQHMNKAKALKVICAKLYEIERRRIQSSRSKLRSEQ 360

Query: 361 IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF 420
           IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF
Sbjct: 361 IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF 420

Query: 421 TSSSS 425
           TSSSS
Sbjct: 421 TSSSS 425

BLAST of Cp4.1LG01g16340 vs. ExPASy TrEMBL
Match: A0A6J1DG80 (uncharacterized protein LOC111019805 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111019805 PE=3 SV=1)

HSP 1 Score: 733 bits (1893), Expect = 3.05e-265
Identity = 385/424 (90.80%), Postives = 399/424 (94.10%), Query Frame = 0

Query: 1   MRIHRPTVRLLEQIRTLALHCRTNPAQICTNPLLPPPSDLLSTSPFRFYSKGEVQSQQLS 60
           MRI+R T+RL+EQIRTLA H RTNP Q   NP LPPP   LST  FRFYSKGE+Q QQLS
Sbjct: 1   MRINRGTLRLVEQIRTLAPHWRTNPVQNTPNPFLPPPFQPLSTPTFRFYSKGEIQPQQLS 60

Query: 61  MELIKIMEQRLKAIELRSSFLLNIINRPEAPPSEYAKANKELKKLRDTMDLINELRSRQK 120
           MELI IME RLKAIELRS+FLLNIINRPEAPP EYAKANKEL+KLRDTMDLINELRSRQK
Sbjct: 61  MELINIMEHRLKAIELRSTFLLNIINRPEAPPVEYAKANKELRKLRDTMDLINELRSRQK 120

Query: 121 EINGLRTLMDECPEDKDMLDMATEELNQAVEEEKRLQHSLLTSLLPRDDADERGCILEVR 180
           EINGLRTLM ECPEDKDMLDMATEEL+QAVEEEKR+Q+ LL SLLP+DDADER CILEVR
Sbjct: 121 EINGLRTLMTECPEDKDMLDMATEELDQAVEEEKRVQNLLLKSLLPKDDADERDCILEVR 180

Query: 181 AGTGGEEASLFAMDIFKMYERYAQKKGWKFEVVDITESDLKGYKEAVAAISGAGVYGKLK 240
           AGTGGEEASLFAMDIFKMYERY Q+KGWKFEVVDITES+LKGYKEAVAAISGAG YGKLK
Sbjct: 181 AGTGGEEASLFAMDIFKMYERYTQRKGWKFEVVDITESELKGYKEAVAAISGAGAYGKLK 240

Query: 241 FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA 300
           FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA
Sbjct: 241 FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA 300

Query: 301 NTTNSAVRVTHIPTGLTVSIQDERSQHMNKAKALKVICAKLYEIERRRIQSSRSKLRSEQ 360
           NTTNSAVRVTHIPTGL+VSIQDERSQHMNKAKALKVICAKLYE ER RIQSSRSKLRSEQ
Sbjct: 301 NTTNSAVRVTHIPTGLSVSIQDERSQHMNKAKALKVICAKLYEKERCRIQSSRSKLRSEQ 360

Query: 361 IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF 420
           IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF
Sbjct: 361 IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF 420

Query: 421 TSSS 424
           TS+S
Sbjct: 421 TSTS 424

BLAST of Cp4.1LG01g16340 vs. ExPASy TrEMBL
Match: A0A6J1DCN5 (uncharacterized protein LOC111019805 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111019805 PE=3 SV=1)

HSP 1 Score: 729 bits (1881), Expect = 2.13e-263
Identity = 385/425 (90.59%), Postives = 399/425 (93.88%), Query Frame = 0

Query: 1   MRIHRPTVRLLEQIRTLALHCRTNPAQICTNPLLPPPSDLLSTSPFRFYSK-GEVQSQQL 60
           MRI+R T+RL+EQIRTLA H RTNP Q   NP LPPP   LST  FRFYSK GE+Q QQL
Sbjct: 1   MRINRGTLRLVEQIRTLAPHWRTNPVQNTPNPFLPPPFQPLSTPTFRFYSKAGEIQPQQL 60

Query: 61  SMELIKIMEQRLKAIELRSSFLLNIINRPEAPPSEYAKANKELKKLRDTMDLINELRSRQ 120
           SMELI IME RLKAIELRS+FLLNIINRPEAPP EYAKANKEL+KLRDTMDLINELRSRQ
Sbjct: 61  SMELINIMEHRLKAIELRSTFLLNIINRPEAPPVEYAKANKELRKLRDTMDLINELRSRQ 120

Query: 121 KEINGLRTLMDECPEDKDMLDMATEELNQAVEEEKRLQHSLLTSLLPRDDADERGCILEV 180
           KEINGLRTLM ECPEDKDMLDMATEEL+QAVEEEKR+Q+ LL SLLP+DDADER CILEV
Sbjct: 121 KEINGLRTLMTECPEDKDMLDMATEELDQAVEEEKRVQNLLLKSLLPKDDADERDCILEV 180

Query: 181 RAGTGGEEASLFAMDIFKMYERYAQKKGWKFEVVDITESDLKGYKEAVAAISGAGVYGKL 240
           RAGTGGEEASLFAMDIFKMYERY Q+KGWKFEVVDITES+LKGYKEAVAAISGAG YGKL
Sbjct: 181 RAGTGGEEASLFAMDIFKMYERYTQRKGWKFEVVDITESELKGYKEAVAAISGAGAYGKL 240

Query: 241 KFESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQH 300
           KFESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQH
Sbjct: 241 KFESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQH 300

Query: 301 ANTTNSAVRVTHIPTGLTVSIQDERSQHMNKAKALKVICAKLYEIERRRIQSSRSKLRSE 360
           ANTTNSAVRVTHIPTGL+VSIQDERSQHMNKAKALKVICAKLYE ER RIQSSRSKLRSE
Sbjct: 301 ANTTNSAVRVTHIPTGLSVSIQDERSQHMNKAKALKVICAKLYEKERCRIQSSRSKLRSE 360

Query: 361 QIGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIAS 420
           QIGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIAS
Sbjct: 361 QIGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIAS 420

Query: 421 FTSSS 424
           FTS+S
Sbjct: 421 FTSTS 425

BLAST of Cp4.1LG01g16340 vs. ExPASy TrEMBL
Match: A0A1S4DW68 (peptide chain release factor 1 OS=Cucumis melo OX=3656 GN=LOC103488835 PE=3 SV=1)

HSP 1 Score: 719 bits (1856), Expect = 1.37e-259
Identity = 375/425 (88.24%), Postives = 401/425 (94.35%), Query Frame = 0

Query: 1   MRIHRPTVRLLEQIRTLALHCRTNPAQICTNPLLPPPSDLLSTSPFRFYSKGEVQSQQLS 60
           MRI+R T+RL+EQIRTLALHCR N ++I  NP LP   D LS+  FRFYSKGEVQSQQLS
Sbjct: 1   MRINRGTMRLVEQIRTLALHCRNNSSRITKNPSLPFHFDPLSSPSFRFYSKGEVQSQQLS 60

Query: 61  MELIKIMEQRLKAIELRSSFLLNIINRPEAPPSEYAKANKELKKLRDTMDLINELRSRQK 120
           MELIKIMEQRL AIELR++FLLNIINRPEAPP++YAKANKELKKLRDTM+LI ELRS+QK
Sbjct: 61  MELIKIMEQRLTAIELRNTFLLNIINRPEAPPADYAKANKELKKLRDTMNLITELRSKQK 120

Query: 121 EINGLRTLMDECPEDKDMLDMATEELNQAVEEEKRLQHSLLTSLLPRDDADERGCILEVR 180
           EINGLRTLM+EC ED+DM DMATEEL QAVEEEKRLQ  LL SLLPRDDADER  ILEVR
Sbjct: 121 EINGLRTLMEECSEDRDMFDMATEELEQAVEEEKRLQQLLLKSLLPRDDADERDSILEVR 180

Query: 181 AGTGGEEASLFAMDIFKMYERYAQKKGWKFEVVDITESDLKGYKEAVAAISGAGVYGKLK 240
           AGTGG+EASLFAMDIFKMYERYAQKKGWKFEVVDITESDLKGYKEA+AAISGAGVYG+LK
Sbjct: 181 AGTGGDEASLFAMDIFKMYERYAQKKGWKFEVVDITESDLKGYKEAIAAISGAGVYGRLK 240

Query: 241 FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA 300
           FESGIHRVQRVPVTEKSGRVHTSA+SVAILPQADEVDVELKNEDLRIDT+RSGGSGGQHA
Sbjct: 241 FESGIHRVQRVPVTEKSGRVHTSAISVAILPQADEVDVELKNEDLRIDTYRSGGSGGQHA 300

Query: 301 NTTNSAVRVTHIPTGLTVSIQDERSQHMNKAKALKVICAKLYEIERRRIQSSRSKLRSEQ 360
           NTTNSAVRV HIPTGLTV+IQDERSQHMNKA+ALK+ICAKLYE+ERRRIQSSRSKLRSEQ
Sbjct: 301 NTTNSAVRVIHIPTGLTVTIQDERSQHMNKARALKLICAKLYEMERRRIQSSRSKLRSEQ 360

Query: 361 IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF 420
           IGSGDRSERIRTYNFPQGRVTDHRVGIT+H+IEEVMQG+NLD FVDALLLQQEMDAIASF
Sbjct: 361 IGSGDRSERIRTYNFPQGRVTDHRVGITDHSIEEVMQGENLDTFVDALLLQQEMDAIASF 420

Query: 421 TSSSS 425
           TSSSS
Sbjct: 421 TSSSS 425

BLAST of Cp4.1LG01g16340 vs. TAIR 10
Match: AT2G47020.1 (Peptide chain release factor 1 )

HSP 1 Score: 579.7 bits (1493), Expect = 1.9e-165
Identity = 295/385 (76.62%), Postives = 349/385 (90.65%), Query Frame = 0

Query: 40  LLSTSPFRFYSKGEVQSQQLSMELIKIMEQRLKAIELRSSFLLNIINRPEAPPSEYAKAN 99
           +LS++  R Y+ G     QLS +LIKIM+QRL AIE R++ L  +IN+PE  P E+++AN
Sbjct: 31  VLSSNMIRLYTTG--MEPQLSPDLIKIMDQRLSAIEHRNAVLQKLINQPEYSPEEFSRAN 90

Query: 100 KELKKLRDTMDLINELRSRQKEINGLRTLMDECPEDKDMLDMATEELNQAVEEEKRLQHS 159
           KEL+KLRD+M LIN+LR++QKEI+GL++L+ E  +DKDMLD+A  EL++AVEEEKRLQ  
Sbjct: 91  KELRKLRDSMLLINDLRAKQKEIDGLKSLVSESSDDKDMLDLAVGELDEAVEEEKRLQTL 150

Query: 160 LLTSLLPRDDADERGCILEVRAGTGGEEASLFAMDIFKMYERYAQKKGWKFEVVDITESD 219
           LL SLLP+D+ADER CILEVRAGTGGEEASLFAMDIF+MYERY+QKKGWKF++VDITESD
Sbjct: 151 LLKSLLPKDEADERDCILEVRAGTGGEEASLFAMDIFRMYERYSQKKGWKFDIVDITESD 210

Query: 220 LKGYKEAVAAISGAGVYGKLKFESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVE 279
           +KGYKEA AAI GA VYGKLKFESGIHRVQR+P+TEKSGR+HTSA+SVAILPQADEVDV+
Sbjct: 211 MKGYKEASAAICGASVYGKLKFESGIHRVQRIPITEKSGRIHTSAISVAILPQADEVDVQ 270

Query: 280 LKNEDLRIDTFRSGGSGGQHANTTNSAVRVTHIPTGLTVSIQDERSQHMNKAKALKVICA 339
           L+NEDLRIDT+RSGGSGGQHANTTNSAVR+ H+PTG+ VSIQDERSQHMN+AKALKV+CA
Sbjct: 271 LRNEDLRIDTYRSGGSGGQHANTTNSAVRIIHLPTGMMVSIQDERSQHMNRAKALKVLCA 330

Query: 340 KLYEIERRRIQSSRSKLRSEQIGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQ 399
           +LYEIER RIQSSRSKLRS+QIGSGDRS RIRTYNFPQGRVTDHRVGIT HAIE++MQG+
Sbjct: 331 RLYEIERLRIQSSRSKLRSDQIGSGDRSGRIRTYNFPQGRVTDHRVGITHHAIEDMMQGE 390

Query: 400 NLDMFVDALLLQQEMDAIASFTSSS 425
           NLDMF+DALLL+QEMDAIASF+S+S
Sbjct: 391 NLDMFIDALLLRQEMDAIASFSSTS 413

BLAST of Cp4.1LG01g16340 vs. TAIR 10
Match: AT2G47020.2 (Peptide chain release factor 1 )

HSP 1 Score: 388.7 bits (997), Expect = 6.2e-108
Identity = 190/245 (77.55%), Postives = 227/245 (92.65%), Query Frame = 0

Query: 87  RPEAPPSEYAKANKELKKLRDTMDLINELRSRQKEINGLRTLMDECPEDKDMLDMATEEL 146
           +PE  P E+++ANKEL+KLRD+M LIN+LR++QKEI+GL++L+ E  +DKDMLD+A  EL
Sbjct: 11  QPEYSPEEFSRANKELRKLRDSMLLINDLRAKQKEIDGLKSLVSESSDDKDMLDLAVGEL 70

Query: 147 NQAVEEEKRLQHSLLTSLLPRDDADERGCILEVRAGTGGEEASLFAMDIFKMYERYAQKK 206
           ++AVEEEKRLQ  LL SLLP+D+ADER CILEVRAGTGGEEASLFAMDIF+MYERY+QKK
Sbjct: 71  DEAVEEEKRLQTLLLKSLLPKDEADERDCILEVRAGTGGEEASLFAMDIFRMYERYSQKK 130

Query: 207 GWKFEVVDITESDLKGYKEAVAAISGAGVYGKLKFESGIHRVQRVPVTEKSGRVHTSAVS 266
           GWKF++VDITESD+KGYKEA AAI GA VYGKLKFESGIHRVQR+P+TEKSGR+HTSA+S
Sbjct: 131 GWKFDIVDITESDMKGYKEASAAICGASVYGKLKFESGIHRVQRIPITEKSGRIHTSAIS 190

Query: 267 VAILPQADEVDVELKNEDLRIDTFRSGGSGGQHANTTNSAVRVTHIPTGLTVSIQDERSQ 326
           VAILPQADEVDV+L+NEDLRIDT+RSGGSGGQHANTTNSAVR+ H+PTG+ VSIQDERSQ
Sbjct: 191 VAILPQADEVDVQLRNEDLRIDTYRSGGSGGQHANTTNSAVRIIHLPTGMMVSIQDERSQ 250

Query: 327 HMNKA 332
           HM+++
Sbjct: 251 HMSQS 255

BLAST of Cp4.1LG01g16340 vs. TAIR 10
Match: AT3G62910.1 (Peptide chain release factor 1 )

HSP 1 Score: 254.2 bits (648), Expect = 1.8e-67
Identity = 136/335 (40.60%), Postives = 210/335 (62.69%), Query Frame = 0

Query: 93  SEYAKANKELKKLRDTMDLINELRSRQKEINGLRTLMDECPEDKDMLDMATEELNQAVEE 152
           SEY K  + + +L + + +    +  +K++   + L  E  +D+DM +M   E+N   +E
Sbjct: 85  SEYQKLAQSMSELDEVVTVFRRFKDCEKQLLESKVLAKEAGDDEDMAEMIGSEINSLTKE 144

Query: 153 EKRLQHSLLTSLLPRDDADERGCILEVRAGTGGEEASLFAMDIFKMYERYAQKKGWKFEV 212
            + L+  L   LLP D  D R  +LEVRAGTGG+EA+++  D+ +MY+RY+++  WKF +
Sbjct: 145 IEELEKQLKVLLLPSDPLDARNILLEVRAGTGGDEAAIWTGDLVRMYQRYSERSSWKFSM 204

Query: 213 VDITESDLKGYKEAVAAISGAGVYGKLKFESGIHRVQRVPVTEKSGRVHTSAVSVAILPQ 272
           V  +E++  GYK  V  I G  VY KLK+ESG+HRVQRVP TE  GRVHTS  +VAI+P+
Sbjct: 205 VSCSEAEHGGYKTCVMEIKGNRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPE 264

Query: 273 ADEVDVELKNEDLRIDTFRSGGSGGQHANTTNSAVRVTHIPTGLTVSIQDERSQHMNKAK 332
           ADEV+V +  +D+ + + RSGG+GGQ+ N   +A+ + H P+G+ +   +ER+Q  NKA+
Sbjct: 265 ADEVEVVIDPKDIELTSARSGGAGGQNVNKVETAIDLFHKPSGIRIFCTEERTQIRNKAR 324

Query: 333 ALKVICAKLYEIERRRIQSSRSKLRSEQIGSGDRSERIRTYNFPQGRVTDHRVGITEHAI 392
           A +++ AKLYEI+ R  Q      R  Q+G+G RSE+IRTYN+   RVTDHR+ +   A+
Sbjct: 325 AFQLLRAKLYEIKVREQQEKIRNERKSQVGTGARSEKIRTYNYKDSRVTDHRLKM-NFAL 384

Query: 393 EEVMQGQNLDMFVDALLLQQE--MDAIASFTSSSS 426
              + G   D       L+Q+  M+ ++   ++SS
Sbjct: 385 TTFLDGALEDAVQACAALEQKELMEELSESVAASS 418

BLAST of Cp4.1LG01g16340 vs. TAIR 10
Match: AT1G56350.1 (Peptide chain release factor 2 )

HSP 1 Score: 170.6 bits (431), Expect = 2.7e-42
Identity = 109/300 (36.33%), Postives = 169/300 (56.33%), Query Frame = 0

Query: 121 EINGLRTLMDECPEDKDMLDMATEELNQAVEEE--------KRLQHSLLTSLLPRDDADE 180
           ++ G+ T   E  E  DML +A EE +  +E E        +R+        L   D D 
Sbjct: 174 KMKGVMTFERELLEHIDMLKLAKEENDSELESETLKALIDMRRVSKEKELEALLSADNDP 233

Query: 181 RGCILEVRAGTGGEEASLFAMDIFKMYERYAQKKGWKFEVVDITESDLKGYKEAVAAISG 240
             C +EV+AG GG E++ +A  + +MY+ +AQ++ +   VVD    ++ G K A   ++G
Sbjct: 234 CSCYIEVQAGAGGTESNDWAAMVMEMYKTWAQRRKFSVTVVDEAPGEIAGIKRATIKVNG 293

Query: 241 AGVYGKLKFESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADE--VDVELKNEDLRIDTF 300
              YG  K E G+HR+ R+   +   R HTS  +VA++P   +    VE+ + DLRI+ F
Sbjct: 294 EYAYGYAKAEVGVHRLVRISPFDSGKRRHTSFAAVAVIPILGDGSTRVEINDSDLRIERF 353

Query: 301 RSGGSGGQHANTTNSAVRVTHIPTGLTVSIQDERSQHMNKAKALKVICAKLYEIERRRIQ 360
           RSGG+GGQHANTT+SAVR+ HIPTG+T + Q+ERSQH NKA A+ V+ ++L ++E  R Q
Sbjct: 354 RSGGAGGQHANTTDSAVRIVHIPTGITATCQNERSQHSNKASAMAVLQSRLDQLEMAR-Q 413

Query: 361 SSRSKLRSEQIGSGDRSERIRTYNF-PQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALL 410
           ++ +   ++ +       +IRTY   P   V D R        + V++G +LD F+ + L
Sbjct: 414 TAMNAQHTQSLTEISWGNQIRTYVLHPYRMVKDLRTNYEVSDPDSVLEG-DLDGFILSFL 471

BLAST of Cp4.1LG01g16340 vs. TAIR 10
Match: AT5G36170.1 (high chlorophyll fluorescent 109 )

HSP 1 Score: 145.6 bits (366), Expect = 9.2e-35
Identity = 107/327 (32.72%), Postives = 170/327 (51.99%), Query Frame = 0

Query: 104 KLRDTMDLINELRSRQKEINGLRTLMDEC------PEDKDMLDMATEELNQAVEEE--KR 163
           K ++T+  +N+L+ R + ++  +T++++        E+ D  D++  E    + +E  K 
Sbjct: 129 KAQETLSSLNDLKDRMRLLSEFKTMVEDAETIVKLTEEMDSTDVSLLEEAMGIIKELNKS 188

Query: 164 LQHSLLTSLLPRDDADERGCILEVRAGTGGEEASLFAMDIFKMYERYAQKKGWKFEVVDI 223
           L    LT LL     D+ G ++ + AG GG +A  +A  + +MY R+ +K+ +K +VV++
Sbjct: 189 LDKFELTQLL-SGPYDKEGAVVYITAGAGGTDAQDWADMLLRMYMRWGEKQRYKTKVVEM 248

Query: 224 TESDLKGYKEAVAAISGAGVYGKLKFESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADE 283
           +  +  G K A   I G   YG +  E G HR+ R       G   TS   V ++P   E
Sbjct: 249 SNGEEAGIKSATLEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPE 308

Query: 284 --VDVELKNEDLRIDTFRSGGSGGQHANTTNSAVRVTHIPTGLTVSIQDERSQHMNKAKA 343
             V +E+  EDL I   R+GG GGQ+ N   +AVR+THIPTG+ V   +ERSQ  NK +A
Sbjct: 309 EAVGIEIPEEDLDISFTRAGGKGGQNVNKVETAVRITHIPTGVAVRCTEERSQLANKTRA 368

Query: 344 LKVICAKLYEIERRRIQSSRSKLRSEQIGSGDRSERIRTYNF-PQGRVTDHRVGITEHAI 403
           L  + AKL  I   +  +   ++R + +   +  ++IR Y F P   V D R G     I
Sbjct: 369 LIRLKAKLMVIAEEQRATEIKEIRGDAV-KAEWGQQIRNYVFHPYKLVKDVRTGHETSDI 428

Query: 404 EEVMQGQNLDMFVDALLLQQEMDAIAS 420
             VM G +LD F+ A L  +   A+AS
Sbjct: 429 TSVMDG-DLDPFIKAYLKHKYTLAMAS 452

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A2RVR72.7e-16476.62Peptide chain release factor 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN... [more]
Q2RWE19.7e-9851.14Peptide chain release factor 1 OS=Rhodospirillum rubrum (strain ATCC 11170 / ATH... [more]
A5V9C38.2e-9749.86Peptide chain release factor 1 OS=Sphingomonas wittichii (strain RW1 / DSM 6014 ... [more]
A7HTY33.1e-9650.00Peptide chain release factor 1 OS=Parvibaculum lavamentivorans (strain DS-1 / DS... [more]
A8GXI14.1e-9654.38Peptide chain release factor 1 OS=Rickettsia bellii (strain OSU 85-389) OX=39189... [more]
Match NameE-valueIdentityDescription
XP_023527687.11.14e-297100.00uncharacterized protein LOC111790812 [Cucurbita pepo subsp. pepo][more]
XP_022957138.13.00e-29398.82uncharacterized protein LOC111458610 [Cucurbita moschata] >KAG6601633.1 hypothet... [more]
XP_022997983.13.00e-29398.59uncharacterized protein LOC111492771 [Cucurbita maxima][more]
XP_038891510.11.30e-27191.76peptide chain release factor 1, mitochondrial [Benincasa hispida][more]
XP_022151971.16.30e-26590.80uncharacterized protein LOC111019805 isoform X2 [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A6J1KFI01.45e-29398.59uncharacterized protein LOC111492771 OS=Cucurbita maxima OX=3661 GN=LOC111492771... [more]
A0A6J1GZD21.45e-29398.82uncharacterized protein LOC111458610 OS=Cucurbita moschata OX=3662 GN=LOC1114586... [more]
A0A6J1DG803.05e-26590.80uncharacterized protein LOC111019805 isoform X2 OS=Momordica charantia OX=3673 G... [more]
A0A6J1DCN52.13e-26390.59uncharacterized protein LOC111019805 isoform X1 OS=Momordica charantia OX=3673 G... [more]
A0A1S4DW681.37e-25988.24peptide chain release factor 1 OS=Cucumis melo OX=3656 GN=LOC103488835 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G47020.11.9e-16576.62Peptide chain release factor 1 [more]
AT2G47020.26.2e-10877.55Peptide chain release factor 1 [more]
AT3G62910.11.8e-6740.60Peptide chain release factor 1 [more]
AT1G56350.12.7e-4236.33Peptide chain release factor 2 [more]
AT5G36170.19.2e-3532.72high chlorophyll fluorescent 109 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 95..115
NoneNo IPR availableCOILSCoilCoilcoord: 139..159
NoneNo IPR availableGENE3D6.10.140.1950coord: 58..169
e-value: 7.2E-17
score: 63.5
NoneNo IPR availableGENE3D3.30.160.20coord: 273..368
e-value: 8.4E-87
score: 291.8
NoneNo IPR availableGENE3D3.30.70.1660coord: 177..405
e-value: 8.4E-87
score: 291.8
NoneNo IPR availablePANTHERPTHR43804LD18447Pcoord: 50..422
NoneNo IPR availablePANTHERPTHR43804:SF7LD18447Pcoord: 50..422
NoneNo IPR availableSUPERFAMILY75620Release factorcoord: 92..412
IPR005139Peptide chain release factorSMARTSM00937PCRF_a_2coord: 127..242
e-value: 2.7E-53
score: 193.1
IPR005139Peptide chain release factorPFAMPF03462PCRFcoord: 92..267
e-value: 3.8E-62
score: 209.5
IPR000352Peptide chain release factor class IPFAMPF00472RF-1coord: 275..384
e-value: 1.7E-36
score: 124.7
IPR004373Peptide chain release factor 1TIGRFAMTIGR00019TIGR00019coord: 69..420
e-value: 5.1E-133
score: 441.4
IPR004373Peptide chain release factor 1HAMAPMF_00093Rel_fac_1coord: 67..421
score: 34.532482

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g16340.1Cp4.1LG01g16340.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006415 translational termination
molecular_function GO:0016149 translation release factor activity, codon specific
molecular_function GO:0003747 translation release factor activity