CmoCh20G004730 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh20G004730
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Description3-ketoacyl-CoA synthase
LocationCmo_Chr20: 2191820 .. 2193205 (+)
RNA-Seq ExpressionCmoCh20G004730
SyntenyCmoCh20G004730
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGCTTCTGAGTTTGTTCTATTTGGGTTCCTTTTTGTTCTTCCTGTTCATCATTTGGAAGCATTTTGATGAAAAAAGACACCAACAATGCTACATATTGGAGTATCAATGCTACAAACCAACAGACGACAGAAAATTAAGCACAGAATTCAGCGGAGACATCATAAAAAGAACCAAAAACCTCGGCCTCAACGAGTACAAATTCCTCCTCAAAGCCGCCGTCAGCTCCGGCATCGGCGAAGAAACCTACGGCCCAAGAATCATGTTCGCCGGCCGTGAAGAATCTCCCACTCTCGACGATGGAATCTCAGAAATGGACGAGTTTTTCCACGACAGCATCCAGAAGCTCTTCGACAAATCAGGCTTCTCCCCGCCGGAAATCGACATCTTAGTCGTAAACGTCTCAATGTTAGCGTCGATTCCGTCGCTCTCCGCTCGGATCATCAACCATTACAAGCTCCGCCCTGATGTCAAGGTGTTCAACCTCACCGGAATGGGCTGCAGCGCTAGTTTAATATCAATCGACGTCGTTTCAAGAGTGTTCAAATCGTATAAAAACATGAACGCCCTCGTTATAACATCTGAATCATTAAGCCCCAATTGGTATTCCGGCAACGACAGATCCATGATCTTGTCCAATTGCTTATTCCGATCCGGCGGATGTGCGATTCTTCTCACAAACAACACGGCGTTGAAAAACAGAGCCATGTTCAAACTCAAGACCCTGGTTCGAACCCACCACGGCGCAAGAGACGAATCCTACGGATGTTGTTACCAAACAGAGGACGACAAAGGCTACATGGGTTTTCACTTAGGTAAAAACCTCCCCAAGGCAGCAACACGAGCCTTCGTCGACAACCTACGGGAAATCGCACCGAAAATTTTACCGACAAGGGAGCTTCTCCAGTTTTTGATTGTTACCACGGTGAAAAAACTAAAGAGAAATGCAAAACAGGGTAGTGGGGTGAAAACCAGTGCGGTGAATTTCAAAACAGGGGTGGATCATTTCTGCATTCACACCGGCGGGAAAGCGGTGATCGACGGGATCGGAGCGAGTTTGGGGCTGGAGAAGTATGACCTGGAGCCGGCGAGGATGACACTGCACCGATTTGGGAACACATCGGCGAGTAGTTTGTGGTATGTTTTGGCGTATATGGAAGCGAAGAAGCGGCTGAAGAAAGGTGGGCGGGTTTTGATGATAAGCTTTGGAGCTGGGTTCAAGTGCAACAGCTGCTTGTGGGAGGTGGTGCGAGATTTGGAAGACGCCAATGTCTGGGAGGATTGTATTCAACGGTATCCACCTGAATCTCTGTCGAACCCATTTCTGGAAAAATATGGTTGGATTCAACAGGAAGACATAGCCACTTTCAAACTCCCTGAGTGA

mRNA sequence

ATGGAGCTTCTGAGTTTGTTCTATTTGGGTTCCTTTTTGTTCTTCCTGTTCATCATTTGGAAGCATTTTGATGAAAAAAGACACCAACAATGCTACATATTGGAGTATCAATGCTACAAACCAACAGACGACAGAAAATTAAGCACAGAATTCAGCGGAGACATCATAAAAAGAACCAAAAACCTCGGCCTCAACGAGTACAAATTCCTCCTCAAAGCCGCCGTCAGCTCCGGCATCGGCGAAGAAACCTACGGCCCAAGAATCATGTTCGCCGGCCGTGAAGAATCTCCCACTCTCGACGATGGAATCTCAGAAATGGACGAGTTTTTCCACGACAGCATCCAGAAGCTCTTCGACAAATCAGGCTTCTCCCCGCCGGAAATCGACATCTTAGTCGTAAACGTCTCAATGTTAGCGTCGATTCCGTCGCTCTCCGCTCGGATCATCAACCATTACAAGCTCCGCCCTGATGTCAAGGTGTTCAACCTCACCGGAATGGGCTGCAGCGCTAGTTTAATATCAATCGACGTCGTTTCAAGAGTGTTCAAATCGTATAAAAACATGAACGCCCTCGTTATAACATCTGAATCATTAAGCCCCAATTGGTATTCCGGCAACGACAGATCCATGATCTTGTCCAATTGCTTATTCCGATCCGGCGGATGTGCGATTCTTCTCACAAACAACACGGCGTTGAAAAACAGAGCCATGTTCAAACTCAAGACCCTGGTTCGAACCCACCACGGCGCAAGAGACGAATCCTACGGATGTTGTTACCAAACAGAGGACGACAAAGGCTACATGGGTTTTCACTTAGGTAAAAACCTCCCCAAGGCAGCAACACGAGCCTTCGTCGACAACCTACGGGAAATCGCACCGAAAATTTTACCGACAAGGGAGCTTCTCCAGTTTTTGATTGTTACCACGGTGAAAAAACTAAAGAGAAATGCAAAACAGGGTAGTGGGGTGAAAACCAGTGCGGTGAATTTCAAAACAGGGGTGGATCATTTCTGCATTCACACCGGCGGGAAAGCGGTGATCGACGGGATCGGAGCGAGTTTGGGGCTGGAGAAGTATGACCTGGAGCCGGCGAGGATGACACTGCACCGATTTGGGAACACATCGGCGAGTAGTTTGTGGTATGTTTTGGCGTATATGGAAGCGAAGAAGCGGCTGAAGAAAGGTGGGCGGGTTTTGATGATAAGCTTTGGAGCTGGGTTCAAGTGCAACAGCTGCTTGTGGGAGGTGGTGCGAGATTTGGAAGACGCCAATGTCTGGGAGGATTGTATTCAACGGTATCCACCTGAATCTCTGTCGAACCCATTTCTGGAAAAATATGGTTGGATTCAACAGGAAGACATAGCCACTTTCAAACTCCCTGAGTGA

Coding sequence (CDS)

ATGGAGCTTCTGAGTTTGTTCTATTTGGGTTCCTTTTTGTTCTTCCTGTTCATCATTTGGAAGCATTTTGATGAAAAAAGACACCAACAATGCTACATATTGGAGTATCAATGCTACAAACCAACAGACGACAGAAAATTAAGCACAGAATTCAGCGGAGACATCATAAAAAGAACCAAAAACCTCGGCCTCAACGAGTACAAATTCCTCCTCAAAGCCGCCGTCAGCTCCGGCATCGGCGAAGAAACCTACGGCCCAAGAATCATGTTCGCCGGCCGTGAAGAATCTCCCACTCTCGACGATGGAATCTCAGAAATGGACGAGTTTTTCCACGACAGCATCCAGAAGCTCTTCGACAAATCAGGCTTCTCCCCGCCGGAAATCGACATCTTAGTCGTAAACGTCTCAATGTTAGCGTCGATTCCGTCGCTCTCCGCTCGGATCATCAACCATTACAAGCTCCGCCCTGATGTCAAGGTGTTCAACCTCACCGGAATGGGCTGCAGCGCTAGTTTAATATCAATCGACGTCGTTTCAAGAGTGTTCAAATCGTATAAAAACATGAACGCCCTCGTTATAACATCTGAATCATTAAGCCCCAATTGGTATTCCGGCAACGACAGATCCATGATCTTGTCCAATTGCTTATTCCGATCCGGCGGATGTGCGATTCTTCTCACAAACAACACGGCGTTGAAAAACAGAGCCATGTTCAAACTCAAGACCCTGGTTCGAACCCACCACGGCGCAAGAGACGAATCCTACGGATGTTGTTACCAAACAGAGGACGACAAAGGCTACATGGGTTTTCACTTAGGTAAAAACCTCCCCAAGGCAGCAACACGAGCCTTCGTCGACAACCTACGGGAAATCGCACCGAAAATTTTACCGACAAGGGAGCTTCTCCAGTTTTTGATTGTTACCACGGTGAAAAAACTAAAGAGAAATGCAAAACAGGGTAGTGGGGTGAAAACCAGTGCGGTGAATTTCAAAACAGGGGTGGATCATTTCTGCATTCACACCGGCGGGAAAGCGGTGATCGACGGGATCGGAGCGAGTTTGGGGCTGGAGAAGTATGACCTGGAGCCGGCGAGGATGACACTGCACCGATTTGGGAACACATCGGCGAGTAGTTTGTGGTATGTTTTGGCGTATATGGAAGCGAAGAAGCGGCTGAAGAAAGGTGGGCGGGTTTTGATGATAAGCTTTGGAGCTGGGTTCAAGTGCAACAGCTGCTTGTGGGAGGTGGTGCGAGATTTGGAAGACGCCAATGTCTGGGAGGATTGTATTCAACGGTATCCACCTGAATCTCTGTCGAACCCATTTCTGGAAAAATATGGTTGGATTCAACAGGAAGACATAGCCACTTTCAAACTCCCTGAGTGA

Protein sequence

MELLSLFYLGSFLFFLFIIWKHFDEKRHQQCYILEYQCYKPTDDRKLSTEFSGDIIKRTKNLGLNEYKFLLKAAVSSGIGEETYGPRIMFAGREESPTLDDGISEMDEFFHDSIQKLFDKSGFSPPEIDILVVNVSMLASIPSLSARIINHYKLRPDVKVFNLTGMGCSASLISIDVVSRVFKSYKNMNALVITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNNTALKNRAMFKLKTLVRTHHGARDESYGCCYQTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAPKILPTRELLQFLIVTTVKKLKRNAKQGSGVKTSAVNFKTGVDHFCIHTGGKAVIDGIGASLGLEKYDLEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKKGGRVLMISFGAGFKCNSCLWEVVRDLEDANVWEDCIQRYPPESLSNPFLEKYGWIQQEDIATFKLPE
Homology
BLAST of CmoCh20G004730 vs. ExPASy Swiss-Prot
Match: Q9SIB2 (3-ketoacyl-CoA synthase 12 OS=Arabidopsis thaliana OX=3702 GN=KCS12 PE=2 SV=1)

HSP 1 Score: 665.2 bits (1715), Expect = 5.3e-190
Identity = 325/473 (68.71%), Postives = 387/473 (81.82%), Query Frame = 0

Query: 1   MELLSLFYLGSFLFFLFIIWKHFDEKRHQQCYILEYQCYKPTDDRKLSTEFSGDIIKRTK 60
           M+LL LF+     +  F IWK  D K+ + CYIL+YQC+KPTDDR +ST+FSG+II R +
Sbjct: 1   MDLLFLFFSLLLSYLFFKIWKLIDSKQDKDCYILDYQCHKPTDDRMVSTQFSGEIIYRNQ 60

Query: 61  NLGLNEYKFLLKAAVSSGIGEETYGPRIMFAGREESPTLDDGISEMDEFFHDSIQKLFDK 120
           NLGL EYKFLLKA VSSGIGE+TY PR++F GREE P+L DGISEM+EF+ DSI KL ++
Sbjct: 61  NLGLTEYKFLLKAIVSSGIGEQTYAPRLVFEGREERPSLQDGISEMEEFYVDSIGKLLER 120

Query: 121 SGFSPPEIDILVVNVSMLASIPSLSARIINHYKLRPDVKVFNLTGMGCSASLISIDVVSR 180
           +  SP +IDILVVNVSML+S PSL++RIINHYK+R DVKVFNLTGMGCSASLIS+D+V  
Sbjct: 121 NQISPKDIDILVVNVSMLSSTPSLASRIINHYKMRDDVKVFNLTGMGCSASLISVDIVKN 180

Query: 181 VFKSYKNMNALVITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNNTALKNRAMFKL 240
           +FKSY N  ALV TSESLSPNWYSGN+RSMIL+NCLFRSGGCAILLTN  +L+ +AMFKL
Sbjct: 181 IFKSYANKLALVATSESLSPNWYSGNNRSMILANCLFRSGGCAILLTNKRSLRKKAMFKL 240

Query: 241 KTLVRTHHGARDESYGCCYQTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAPKILPTRE 300
           K +VRTHHGAR+ESY CC Q ED++G +GF+LGKNLPKAATRAFV+NL+ I PKILP  E
Sbjct: 241 KCMVRTHHGAREESYNCCIQAEDEQGRVGFYLGKNLPKAATRAFVENLKVITPKILPVTE 300

Query: 301 LLQFLIVTTVKKLK--RNAKQGS-----GVKTSA-VNFKTGVDHFCIHTGGKAVIDGIGA 360
           L++F++   +KK+K  +N  +GS     G    A +NFKTG++HFCIHTGGKAVIDGIG 
Sbjct: 301 LIRFMLKLLIKKIKIRQNPSKGSTNLPPGTPLKAGINFKTGIEHFCIHTGGKAVIDGIGH 360

Query: 361 SLGLEKYDLEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKKGGRVLMISFGAGFKCNSC 420
           SL L +YD+EPARMTLHRFGNTSASSLWYVLAYMEAKKRLK+G RV MISFGAGFKCNSC
Sbjct: 361 SLDLNEYDIEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKRGDRVFMISFGAGFKCNSC 420

Query: 421 LWEVVRDL----EDANVWEDCIQRYPPESLSNPFLEKYGWIQQEDIATFKLPE 462
           +WEVVRDL       NVW  CI  YPP+S+ NP+LEK+GWIQ ED  TFK+P+
Sbjct: 421 VWEVVRDLTGGESKGNVWNHCIDDYPPKSILNPYLEKFGWIQDEDPDTFKVPD 473

BLAST of CmoCh20G004730 vs. ExPASy Swiss-Prot
Match: Q9LQP8 (3-ketoacyl-CoA synthase 3 OS=Arabidopsis thaliana OX=3702 GN=KCS3 PE=2 SV=3)

HSP 1 Score: 652.5 bits (1682), Expect = 3.5e-186
Identity = 320/476 (67.23%), Postives = 380/476 (79.83%), Query Frame = 0

Query: 1   MELLSLFYLGSFLFFLFIIWKHFDEKRHQQCYILEYQCYKPTDDRKLSTEFSGDIIKRTK 60
           M+LL +       + +F IWK  D KR Q CYIL+YQC+KP+DDR ++T+FSGDII R K
Sbjct: 1   MDLLVMLLSLLVSYLIFKIWKRIDSKRDQNCYILDYQCHKPSDDRMVNTQFSGDIILRNK 60

Query: 61  NLGLNEYKFLLKAAVSSGIGEETYGPRIMFAGREESPTLDDGISEMDEFFHDSIQKLFDK 120
           +L LNEYKFLLKA VSSGIGE+TY PR+ F GRE+ PTL DG+SEM+EF+ D+I+K+  +
Sbjct: 61  HLRLNEYKFLLKAIVSSGIGEQTYAPRLFFEGREQRPTLQDGLSEMEEFYIDTIEKVLKR 120

Query: 121 SGFSPPEIDILVVNVSMLASIPSLSARIINHYKLRPDVKVFNLTGMGCSASLISIDVVSR 180
           +  SP EIDILVVNVSML S PSLSARIINHYK+R D+KVFNLT MGCSAS+ISID+V  
Sbjct: 121 NKISPSEIDILVVNVSMLNSTPSLSARIINHYKMREDIKVFNLTAMGCSASVISIDIVKN 180

Query: 181 VFKSYKNMNALVITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNNTALKNRAMFKL 240
           +FK+YKN  ALV+TSESLSPNWYSGN+RSMIL+NCLFRSGGCA+LLTN  +L  RAMFKL
Sbjct: 181 IFKTYKNKLALVVTSESLSPNWYSGNNRSMILANCLFRSGGCAVLLTNKRSLSRRAMFKL 240

Query: 241 KTLVRTHHGARDESYGCCYQTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAPKILPTRE 300
           + LVRTHHGARD+S+  C Q ED+ G++G HL K LPKAATRAF+DNL+ I PKILP  E
Sbjct: 241 RCLVRTHHGARDDSFNACVQKEDELGHIGVHLDKTLPKAATRAFIDNLKVITPKILPVTE 300

Query: 301 LLQFLIVTTVKKLKRNAKQGS----------GVKTSAVNFKTGVDHFCIHTGGKAVIDGI 360
           LL+F++   +KKL+ +  +GS          GVK + +NFKTG+DHFCIHTGGKAVID I
Sbjct: 301 LLRFMLCLLLKKLRSSPSKGSTNVTQAAPKAGVK-AGINFKTGIDHFCIHTGGKAVIDAI 360

Query: 361 GASLGLEKYDLEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKKGGRVLMISFGAGFKCN 420
           G SL L +YDLEPARMTLHRFGNTSASSLWYVL YMEAKKRLK+G RV MISFGAGFKCN
Sbjct: 361 GYSLDLNEYDLEPARMTLHRFGNTSASSLWYVLGYMEAKKRLKRGDRVFMISFGAGFKCN 420

Query: 421 SCLWEVVRDLE----DANVWEDCIQRYPPESLSNPFLEKYGWI-QQEDIATFKLPE 462
           SC+WEVVRDL       NVW  CI +YPP+S+ NPF EKYGWI ++ED  TFK+PE
Sbjct: 421 SCVWEVVRDLNVGEAVGNVWNHCINQYPPKSILNPFFEKYGWIHEEEDPDTFKMPE 475

BLAST of CmoCh20G004730 vs. ExPASy Swiss-Prot
Match: Q9LZ72 (3-ketoacyl-CoA synthase 19 OS=Arabidopsis thaliana OX=3702 GN=KCS19 PE=2 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 6.7e-145
Identity = 249/460 (54.13%), Postives = 340/460 (73.91%), Query Frame = 0

Query: 4   LSLFYLGSFL----FFLFIIWKHFDEKRHQQ-CYILEYQCYKPTDDRKLSTEFSGDIIKR 63
           + LF L S L     F+F I+K   ++R+Q+ CY+L Y+CYK  ++RKL TE    +++R
Sbjct: 1   MELFSLSSLLLLSTLFVFYIFKFVFKRRNQRNCYMLHYECYKGMEERKLDTETCAKVVQR 60

Query: 64  TKNLGLNEYKFLLKAAVSSGIGEETYGPRIMFAGREESPTLDDGISEMDEFFHDSIQKLF 123
            KNLGL EY+FLL+   SSGIGEETYGPR +  GRE+SPTL D  SEMDE   D++ KLF
Sbjct: 61  NKNLGLEEYRFLLRTMASSGIGEETYGPRNVLEGREDSPTLLDAHSEMDEIMFDTLDKLF 120

Query: 124 DKS--GFSPPEIDILVVNVSMLASIPSLSARIINHYKLRPDVKVFNLTGMGCSASLISID 183
            K+    SP +IDILVVNVS+ A  PSL++R+IN YK+R D+K +NL+G+GCSAS+ISID
Sbjct: 121 HKTKGSISPSDIDILVVNVSLFAPSPSLTSRVINRYKMREDIKSYNLSGLGCSASVISID 180

Query: 184 VVSRVFKSYKNMNALVITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNNTALKNRA 243
           +V R+F++ +N  ALV+++E++ P+WY G DRSM+LSNCLFR+GG ++LLTN    KN+A
Sbjct: 181 IVQRMFETRENALALVVSTETMGPHWYCGKDRSMMLSNCLFRAGGSSVLLTNAARFKNQA 240

Query: 244 MFKLKTLVRTHHGARDESYGCCYQTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAPKIL 303
           + KL T+VR H G+ DE+Y CC Q ED  G+ GF L K L KAA RA   NL+ + P++L
Sbjct: 241 LMKLVTVVRAHVGSDDEAYSCCIQMEDRDGHPGFLLTKYLKKAAARALTKNLQVLLPRVL 300

Query: 304 PTRELLQFLIVTTVKKLKRNAKQGSGVKTSAVNFKTGVDHFCIHTGGKAVIDGIGASLGL 363
           P +EL+++ IV  +K+ + +AK+        +N KTG+ HFCIH GG+A+I+G+G SLGL
Sbjct: 301 PVKELIRYAIVRALKR-RTSAKREPASSGIGLNLKTGLQHFCIHPGGRAIIEGVGKSLGL 360

Query: 364 EKYDLEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKKGGRVLMISFGAGFKCNSCLWEV 423
            ++D+EPARM LHRFGNTS+  LWYVL YMEAK RLKKG ++LM+S GAGF+ N+C+WEV
Sbjct: 361 TEFDIEPARMALHRFGNTSSGGLWYVLGYMEAKNRLKKGEKILMMSMGAGFESNNCVWEV 420

Query: 424 VRDLEDANVWEDCIQRYPPES-LSNPFLEKYGWIQQEDIA 456
           ++DL+D NVWED + RYP  S + NPF+EKY WI  + ++
Sbjct: 421 LKDLDDKNVWEDSVDRYPELSRIPNPFVEKYDWINDDTMS 459

BLAST of CmoCh20G004730 vs. ExPASy Swiss-Prot
Match: Q9MAM3 (3-ketoacyl-CoA synthase 1 OS=Arabidopsis thaliana OX=3702 GN=KCS1 PE=1 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 3.2e-94
Identity = 175/429 (40.79%), Postives = 264/429 (61.54%), Query Frame = 0

Query: 13  LFFLFIIWKHFDEKRHQQCYILEYQCYKPTDDRKLSTEFSGDIIKRTKNLGLNEYKFLLK 72
           L FL  +   +   R +  Y++++ CYKP D+RK+S +    + +   +   +  +F  +
Sbjct: 104 LVFLSFVLTLYVANRSKPVYLVDFSCYKPEDERKISVDSFLTMTEENGSFTDDTVQFQQR 163

Query: 73  AAVSSGIGEETYGPRIMFAGREESP---TLDDGISEMDEFFHDSIQKLFDKSGFSPPEID 132
            +  +G+G+ETY PR    G   +P    + +  +E +     ++  LF+K+G  P E+ 
Sbjct: 164 ISNRAGLGDETYLPR----GITSTPPKLNMSEARAEAEAVMFGALDSLFEKTGIKPAEVG 223

Query: 133 ILVVNVSMLASIPSLSARIINHYKLRPDVKVFNLTGMGCSASLISIDVVSRVFKSYKNMN 192
           IL+VN S+    PSLSA I+NHYK+R D+K +NL GMGCSA LISID+ + + K+  N  
Sbjct: 224 ILIVNCSLFNPTPSLSAMIVNHYKMREDIKSYNLGGMGCSAGLISIDLANNLLKANPNSY 283

Query: 193 ALVITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNNTALKNRAMFKLKTLVRTHHG 252
           A+V+++E+++ NWY GNDRSM+L NC+FR GG AILL+N    + ++ + L  +VRTH G
Sbjct: 284 AVVVSTENITLNWYFGNDRSMLLCNCIFRMGGAAILLSNRRQDRKKSKYSLVNVVRTHKG 343

Query: 253 ARDESYGCCYQTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAPKILPTRELLQFLIVTT 312
           + D++Y C YQ ED++G +G  L + L   A  A   N+  + P +LP  E L FLI   
Sbjct: 344 SDDKNYNCVYQKEDERGTIGVSLARELMSVAGDALKTNITTLGPMVLPLSEQLMFLISLV 403

Query: 313 VKKLKRNAKQGSGVKTSAVNFKTGVDHFCIHTGGKAVIDGIGASLGLEKYDLEPARMTLH 372
            +K+ +       VK    +FK   +HFCIH GG+AV+D +  +L L+ + +EP+RMTLH
Sbjct: 404 KRKMFK-----LKVKPYIPDFKLAFEHFCIHAGGRAVLDEVQKNLDLKDWHMEPSRMTLH 463

Query: 373 RFGNTSASSLWYVLAYMEAKKRLKKGGRVLMISFGAGFKCNSCLWEVVRDLE----DANV 432
           RFGNTS+SSLWY +AY EAK R+K G R+  I+FG+GFKCNS +W+ +R +       N 
Sbjct: 464 RFGNTSSSSLWYEMAYTEAKGRVKAGDRLWQIAFGSGFKCNSAVWKALRPVSTEEMTGNA 523

Query: 433 WEDCIQRYP 435
           W   I +YP
Sbjct: 524 WAGSIDQYP 523

BLAST of CmoCh20G004730 vs. ExPASy Swiss-Prot
Match: Q9ZUZ0 (3-ketoacyl-CoA synthase 13 OS=Arabidopsis thaliana OX=3702 GN=HIC PE=2 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 4.3e-91
Identity = 169/427 (39.58%), Postives = 260/427 (60.89%), Query Frame = 0

Query: 13  LFFLFIIWKHFDEKRHQQCYILEYQCYKPTDDRKLSTEFSGDIIKRTKNLGLNEYKFLLK 72
           L  + I +  +   R +  Y++++ C++PTD  K+S+E   ++ K  +       +F+ +
Sbjct: 42  LLLISIFFYAYSTTRSKPVYLVDFSCHQPTDSCKISSETFFNMAKGAQLYTDETIQFMTR 101

Query: 73  AAVSSGIGEETYGPRIMFAGREESPTLDDGISEMDEFFHDSIQKLFDKSGFSPPEIDILV 132
               SG+G++TY PR M      +P++ +   E +     ++  LF K+G  P E+ I +
Sbjct: 102 ILNRSGLGDDTYSPRCMLTS-PPTPSMYEARHESELVIFGALNSLFKKTGIEPREVGIFI 161

Query: 133 VNVSMLASIPSLSARIINHYKLRPDVKVFNLTGMGCSASLISIDVVSRVFKSYKNMNALV 192
           VN S+    PSLS+ I+N YKL+ DVK +NL+GMGCSA  IS+D+ + + K+  N  A++
Sbjct: 162 VNCSLFNPNPSLSSMIVNRYKLKTDVKTYNLSGMGCSAGAISVDLATNLLKANPNTYAVI 221

Query: 193 ITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNNTALKNRAMFKLKTLVRTHHGARD 252
           +++E+++ + Y GNDRSM++ NCLFR GG A++L+N +  + R+ ++L  +VRTH G+ D
Sbjct: 222 VSTENMTLSMYRGNDRSMLVPNCLFRVGGAAVMLSNRSQDRVRSKYELTHIVRTHKGSSD 281

Query: 253 ESYGCCYQTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAPKILPTRELLQFLIVTTVKK 312
           + Y C  Q ED KG +G  L K L   A  +   NL  + P +LP  E L+F++     K
Sbjct: 282 KHYTCAEQKEDSKGIVGVALSKELTVVAGDSLKTNLTALGPLVLPLSEKLRFILFLVKSK 341

Query: 313 LKRNAKQGSGVKTSAVNFKTGVDHFCIHTGGKAVIDGIGASLGLEKYDLEPARMTLHRFG 372
           L R       V     +FK    HFCIH GG+A++D +   LGL ++DLEP+RMTLHRFG
Sbjct: 342 LFR-----LKVSPYVPDFKLCFKHFCIHAGGRALLDAVEKGLGLSEFDLEPSRMTLHRFG 401

Query: 373 NTSASSLWYVLAYMEAKKRLKKGGRVLMISFGAGFKCNSCLWEVVRDLED-----ANVWE 432
           NTS+SSLWY LAY+EAK R+K+G RV  ++FG+GFKCNS +W  +R +        N W 
Sbjct: 402 NTSSSSLWYELAYVEAKCRVKRGDRVWQLAFGSGFKCNSIVWRALRTIPANESLVGNPWG 461

Query: 433 DCIQRYP 435
           D + +YP
Sbjct: 462 DSVHKYP 462

BLAST of CmoCh20G004730 vs. ExPASy TrEMBL
Match: A0A6J1FSZ5 (3-ketoacyl-CoA synthase OS=Cucurbita moschata OX=3662 GN=LOC111448188 PE=3 SV=1)

HSP 1 Score: 935.6 bits (2417), Expect = 7.7e-269
Identity = 461/461 (100.00%), Postives = 461/461 (100.00%), Query Frame = 0

Query: 1   MELLSLFYLGSFLFFLFIIWKHFDEKRHQQCYILEYQCYKPTDDRKLSTEFSGDIIKRTK 60
           MELLSLFYLGSFLFFLFIIWKHFDEKRHQQCYILEYQCYKPTDDRKLSTEFSGDIIKRTK
Sbjct: 1   MELLSLFYLGSFLFFLFIIWKHFDEKRHQQCYILEYQCYKPTDDRKLSTEFSGDIIKRTK 60

Query: 61  NLGLNEYKFLLKAAVSSGIGEETYGPRIMFAGREESPTLDDGISEMDEFFHDSIQKLFDK 120
           NLGLNEYKFLLKAAVSSGIGEETYGPRIMFAGREESPTLDDGISEMDEFFHDSIQKLFDK
Sbjct: 61  NLGLNEYKFLLKAAVSSGIGEETYGPRIMFAGREESPTLDDGISEMDEFFHDSIQKLFDK 120

Query: 121 SGFSPPEIDILVVNVSMLASIPSLSARIINHYKLRPDVKVFNLTGMGCSASLISIDVVSR 180
           SGFSPPEIDILVVNVSMLASIPSLSARIINHYKLRPDVKVFNLTGMGCSASLISIDVVSR
Sbjct: 121 SGFSPPEIDILVVNVSMLASIPSLSARIINHYKLRPDVKVFNLTGMGCSASLISIDVVSR 180

Query: 181 VFKSYKNMNALVITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNNTALKNRAMFKL 240
           VFKSYKNMNALVITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNNTALKNRAMFKL
Sbjct: 181 VFKSYKNMNALVITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNNTALKNRAMFKL 240

Query: 241 KTLVRTHHGARDESYGCCYQTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAPKILPTRE 300
           KTLVRTHHGARDESYGCCYQTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAPKILPTRE
Sbjct: 241 KTLVRTHHGARDESYGCCYQTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAPKILPTRE 300

Query: 301 LLQFLIVTTVKKLKRNAKQGSGVKTSAVNFKTGVDHFCIHTGGKAVIDGIGASLGLEKYD 360
           LLQFLIVTTVKKLKRNAKQGSGVKTSAVNFKTGVDHFCIHTGGKAVIDGIGASLGLEKYD
Sbjct: 301 LLQFLIVTTVKKLKRNAKQGSGVKTSAVNFKTGVDHFCIHTGGKAVIDGIGASLGLEKYD 360

Query: 361 LEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKKGGRVLMISFGAGFKCNSCLWEVVRDL 420
           LEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKKGGRVLMISFGAGFKCNSCLWEVVRDL
Sbjct: 361 LEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKKGGRVLMISFGAGFKCNSCLWEVVRDL 420

Query: 421 EDANVWEDCIQRYPPESLSNPFLEKYGWIQQEDIATFKLPE 462
           EDANVWEDCIQRYPPESLSNPFLEKYGWIQQEDIATFKLPE
Sbjct: 421 EDANVWEDCIQRYPPESLSNPFLEKYGWIQQEDIATFKLPE 461

BLAST of CmoCh20G004730 vs. ExPASy TrEMBL
Match: A0A6J1JHS6 (3-ketoacyl-CoA synthase OS=Cucurbita maxima OX=3661 GN=LOC111484475 PE=3 SV=1)

HSP 1 Score: 922.2 bits (2382), Expect = 8.8e-265
Identity = 452/461 (98.05%), Postives = 455/461 (98.70%), Query Frame = 0

Query: 1   MELLSLFYLGSFLFFLFIIWKHFDEKRHQQCYILEYQCYKPTDDRKLSTEFSGDIIKRTK 60
           MELLSLFYLGSFLFFLFIIWKHFDEKRHQQCYILEYQCYKPTDDRKLSTEFSGDIIKRTK
Sbjct: 1   MELLSLFYLGSFLFFLFIIWKHFDEKRHQQCYILEYQCYKPTDDRKLSTEFSGDIIKRTK 60

Query: 61  NLGLNEYKFLLKAAVSSGIGEETYGPRIMFAGREESPTLDDGISEMDEFFHDSIQKLFDK 120
           NLGLNEYKF LKAAVSSGIGEETYGPRIMFAGREESPTLDDGISEMDEFFHDSIQKLFDK
Sbjct: 61  NLGLNEYKFFLKAAVSSGIGEETYGPRIMFAGREESPTLDDGISEMDEFFHDSIQKLFDK 120

Query: 121 SGFSPPEIDILVVNVSMLASIPSLSARIINHYKLRPDVKVFNLTGMGCSASLISIDVVSR 180
           SGFSPPEIDILVVNVSMLASIPSLSARIINHYKLRPD+KVFNLTGMGCSASLISIDVVSR
Sbjct: 121 SGFSPPEIDILVVNVSMLASIPSLSARIINHYKLRPDIKVFNLTGMGCSASLISIDVVSR 180

Query: 181 VFKSYKNMNALVITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNNTALKNRAMFKL 240
           VFKSYKN+NALVITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNNT LKNRAMFKL
Sbjct: 181 VFKSYKNVNALVITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNNTTLKNRAMFKL 240

Query: 241 KTLVRTHHGARDESYGCCYQTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAPKILPTRE 300
           KTLVRTHHGA+DESY CCYQTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAPKILPTRE
Sbjct: 241 KTLVRTHHGAKDESYSCCYQTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAPKILPTRE 300

Query: 301 LLQFLIVTTVKKLKRNAKQGSGVKTSAVNFKTGVDHFCIHTGGKAVIDGIGASLGLEKYD 360
           LLQFLIVTTVKKLKRN KQG GVKTSAVNFKTGVDHFCIHTGGKAVIDGIG SLGLEKYD
Sbjct: 301 LLQFLIVTTVKKLKRNGKQGRGVKTSAVNFKTGVDHFCIHTGGKAVIDGIGVSLGLEKYD 360

Query: 361 LEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKKGGRVLMISFGAGFKCNSCLWEVVRDL 420
           LEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKKGGRVLMISFGAGFKCNSCLWEVVRDL
Sbjct: 361 LEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKKGGRVLMISFGAGFKCNSCLWEVVRDL 420

Query: 421 EDANVWEDCIQRYPPESLSNPFLEKYGWIQQEDIATFKLPE 462
           EDANVWEDCIQRYPPESLSNPFLEKYGWIQQEDIATFKLPE
Sbjct: 421 EDANVWEDCIQRYPPESLSNPFLEKYGWIQQEDIATFKLPE 461

BLAST of CmoCh20G004730 vs. ExPASy TrEMBL
Match: A0A5A7V8E0 (3-ketoacyl-CoA synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold250G00340 PE=3 SV=1)

HSP 1 Score: 846.7 bits (2186), Expect = 4.7e-242
Identity = 411/461 (89.15%), Postives = 434/461 (94.14%), Query Frame = 0

Query: 1   MELLSLFYLGSFLFFLFIIWKHFDEKRHQQCYILEYQCYKPTDDRKLSTEFSGDIIKRTK 60
           MELL  F+L S  +FLF IWK+FD+KR Q+CYIL YQCYKPTDDRKLSTEF G++IKRTK
Sbjct: 1   MELLCFFFLCSVFYFLFTIWKYFDDKRDQECYILNYQCYKPTDDRKLSTEFCGEVIKRTK 60

Query: 61  NLGLNEYKFLLKAAVSSGIGEETYGPRIMFAGREESPTLDDGISEMDEFFHDSIQKLFDK 120
           NLGLNEYKFLLKA VSSGIGEETYGPRIMFAGRE+SPTLDDGISEMDEFFHDSI K+F K
Sbjct: 61  NLGLNEYKFLLKAIVSSGIGEETYGPRIMFAGREDSPTLDDGISEMDEFFHDSIGKIFQK 120

Query: 121 SGFSPPEIDILVVNVSMLASIPSLSARIINHYKLRPDVKVFNLTGMGCSASLISIDVVSR 180
           SGFSP +ID+LVVNVSMLA+IPSLSARIIN+YKLR DVKVFNLTGMGCSASLISIDVVSR
Sbjct: 121 SGFSPSDIDVLVVNVSMLATIPSLSARIINYYKLRSDVKVFNLTGMGCSASLISIDVVSR 180

Query: 181 VFKSYKNMNALVITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNNTALKNRAMFKL 240
           VFKSYKN+NALVITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNN ALKNRAMFKL
Sbjct: 181 VFKSYKNINALVITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNNRALKNRAMFKL 240

Query: 241 KTLVRTHHGARDESYGCCYQTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAPKILPTRE 300
           K L+RTHHGARDESYGCC QTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAP+ILP RE
Sbjct: 241 KCLIRTHHGARDESYGCCIQTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAPRILPARE 300

Query: 301 LLQFLIVTTVKKLKRNAKQGSGVKTSAVNFKTGVDHFCIHTGGKAVIDGIGASLGLEKYD 360
           L+QFLI++  KKL RN KQGSG KTSAVNFKTGVDHFCIHTGGKAVIDGIG SLGLEKYD
Sbjct: 301 LVQFLILSLGKKLNRNPKQGSGPKTSAVNFKTGVDHFCIHTGGKAVIDGIGLSLGLEKYD 360

Query: 361 LEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKKGGRVLMISFGAGFKCNSCLWEVVRDL 420
           LEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKKG R+LMISFGAGFKCNSCLWEVVRDL
Sbjct: 361 LEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKKGERILMISFGAGFKCNSCLWEVVRDL 420

Query: 421 EDANVWEDCIQRYPPESLSNPFLEKYGWIQQEDIATFKLPE 462
           ED NVWEDCI+ YPPES+SNPFLEKYGWIQQEDIATFK+PE
Sbjct: 421 EDGNVWEDCIEHYPPESISNPFLEKYGWIQQEDIATFKMPE 461

BLAST of CmoCh20G004730 vs. ExPASy TrEMBL
Match: A0A1S3BJH0 (3-ketoacyl-CoA synthase OS=Cucumis melo OX=3656 GN=LOC103490572 PE=3 SV=1)

HSP 1 Score: 846.7 bits (2186), Expect = 4.7e-242
Identity = 411/461 (89.15%), Postives = 434/461 (94.14%), Query Frame = 0

Query: 1   MELLSLFYLGSFLFFLFIIWKHFDEKRHQQCYILEYQCYKPTDDRKLSTEFSGDIIKRTK 60
           MELL  F+L S  +FLF IWK+FD+KR Q+CYIL YQCYKPTDDRKLSTEF G++IKRTK
Sbjct: 1   MELLCFFFLCSVFYFLFTIWKYFDDKRDQECYILNYQCYKPTDDRKLSTEFCGEVIKRTK 60

Query: 61  NLGLNEYKFLLKAAVSSGIGEETYGPRIMFAGREESPTLDDGISEMDEFFHDSIQKLFDK 120
           NLGLNEYKFLLKA VSSGIGEETYGPRIMFAGRE+SPTLDDGISEMDEFFHDSI K+F K
Sbjct: 61  NLGLNEYKFLLKAIVSSGIGEETYGPRIMFAGREDSPTLDDGISEMDEFFHDSIGKIFQK 120

Query: 121 SGFSPPEIDILVVNVSMLASIPSLSARIINHYKLRPDVKVFNLTGMGCSASLISIDVVSR 180
           SGFSP +ID+LVVNVSMLA+IPSLSARIIN+YKLR DVKVFNLTGMGCSASLISIDVVSR
Sbjct: 121 SGFSPSDIDVLVVNVSMLATIPSLSARIINYYKLRSDVKVFNLTGMGCSASLISIDVVSR 180

Query: 181 VFKSYKNMNALVITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNNTALKNRAMFKL 240
           VFKSYKN+NALVITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNN ALKNRAMFKL
Sbjct: 181 VFKSYKNINALVITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNNRALKNRAMFKL 240

Query: 241 KTLVRTHHGARDESYGCCYQTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAPKILPTRE 300
           K L+RTHHGARDESYGCC QTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAP+ILP RE
Sbjct: 241 KCLIRTHHGARDESYGCCIQTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAPRILPARE 300

Query: 301 LLQFLIVTTVKKLKRNAKQGSGVKTSAVNFKTGVDHFCIHTGGKAVIDGIGASLGLEKYD 360
           L+QFLI++  KKL RN KQGSG KTSAVNFKTGVDHFCIHTGGKAVIDGIG SLGLEKYD
Sbjct: 301 LVQFLILSLGKKLNRNPKQGSGPKTSAVNFKTGVDHFCIHTGGKAVIDGIGLSLGLEKYD 360

Query: 361 LEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKKGGRVLMISFGAGFKCNSCLWEVVRDL 420
           LEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKKG R+LMISFGAGFKCNSCLWEVVRDL
Sbjct: 361 LEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKKGERILMISFGAGFKCNSCLWEVVRDL 420

Query: 421 EDANVWEDCIQRYPPESLSNPFLEKYGWIQQEDIATFKLPE 462
           ED NVWEDCI+ YPPES+SNPFLEKYGWIQQEDIATFK+PE
Sbjct: 421 EDGNVWEDCIEHYPPESISNPFLEKYGWIQQEDIATFKMPE 461

BLAST of CmoCh20G004730 vs. ExPASy TrEMBL
Match: A0A6J1KYJ7 (3-ketoacyl-CoA synthase OS=Cucurbita maxima OX=3661 GN=LOC111499896 PE=3 SV=1)

HSP 1 Score: 842.8 bits (2176), Expect = 6.8e-241
Identity = 406/461 (88.07%), Postives = 431/461 (93.49%), Query Frame = 0

Query: 1   MELLSLFYLGSFLFFLFIIWKHFDEKRHQQCYILEYQCYKPTDDRKLSTEFSGDIIKRTK 60
           MELLS FY  SFL+FLF IWKHFDEKRHQ+CYIL YQCYKPTDDRKL+TEFSGD+IKRTK
Sbjct: 1   MELLSFFYFCSFLYFLFTIWKHFDEKRHQECYILSYQCYKPTDDRKLNTEFSGDVIKRTK 60

Query: 61  NLGLNEYKFLLKAAVSSGIGEETYGPRIMFAGREESPTLDDGISEMDEFFHDSIQKLFDK 120
           NLGLNEYKFLLKA VSSGIGE+TYGPRIMFA RE+SPTLDDGISEMDEFFHD+IQKLFDK
Sbjct: 61  NLGLNEYKFLLKAVVSSGIGEQTYGPRIMFAAREDSPTLDDGISEMDEFFHDTIQKLFDK 120

Query: 121 SGFSPPEIDILVVNVSMLASIPSLSARIINHYKLRPDVKVFNLTGMGCSASLISIDVVSR 180
           SGFSP +ID+LVVNVSML+SIPSLSARIINHYKLR DVKVFNLTGMGCSASLIS+D+VSR
Sbjct: 121 SGFSPSDIDVLVVNVSMLSSIPSLSARIINHYKLRHDVKVFNLTGMGCSASLISVDIVSR 180

Query: 181 VFKSYKNMNALVITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNNTALKNRAMFKL 240
           VFKS+KN+NALV+TSESLSPNWYSGND+SMILSNCLFRSGGC ILLTN TALKNRAMFKL
Sbjct: 181 VFKSHKNINALVVTSESLSPNWYSGNDKSMILSNCLFRSGGCVILLTNKTALKNRAMFKL 240

Query: 241 KTLVRTHHGARDESYGCCYQTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAPKILPTRE 300
           K L+RTHHGARDESYGCC QTEDDKG  GFHLGKNLPKAATRAFVDNLREIAPKILP RE
Sbjct: 241 KCLIRTHHGARDESYGCCIQTEDDKGNTGFHLGKNLPKAATRAFVDNLREIAPKILPIRE 300

Query: 301 LLQFLIVTTVKKLKRNAKQGSGVKTSAVNFKTGVDHFCIHTGGKAVIDGIGASLGLEKYD 360
           LLQFL+++ +KKLK N KQ  G KTSAV+FKTGVDHFCIHTGGKAVIDGIG SLGLEKYD
Sbjct: 301 LLQFLVMSFMKKLKGNRKQSGGAKTSAVDFKTGVDHFCIHTGGKAVIDGIGVSLGLEKYD 360

Query: 361 LEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKKGGRVLMISFGAGFKCNSCLWEVVRDL 420
           LEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKKG RVLMISFGAGFKCNSCLWEV RDL
Sbjct: 361 LEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKKGERVLMISFGAGFKCNSCLWEVARDL 420

Query: 421 EDANVWEDCIQRYPPESLSNPFLEKYGWIQQEDIATFKLPE 462
           +  NVW+DCI  YPPESL+NPFLEKYGWIQ EDIATFKLPE
Sbjct: 421 DGGNVWDDCIDEYPPESLANPFLEKYGWIQHEDIATFKLPE 461

BLAST of CmoCh20G004730 vs. NCBI nr
Match: XP_022943417.1 (3-ketoacyl-CoA synthase 12-like [Cucurbita moschata])

HSP 1 Score: 935.6 bits (2417), Expect = 1.6e-268
Identity = 461/461 (100.00%), Postives = 461/461 (100.00%), Query Frame = 0

Query: 1   MELLSLFYLGSFLFFLFIIWKHFDEKRHQQCYILEYQCYKPTDDRKLSTEFSGDIIKRTK 60
           MELLSLFYLGSFLFFLFIIWKHFDEKRHQQCYILEYQCYKPTDDRKLSTEFSGDIIKRTK
Sbjct: 1   MELLSLFYLGSFLFFLFIIWKHFDEKRHQQCYILEYQCYKPTDDRKLSTEFSGDIIKRTK 60

Query: 61  NLGLNEYKFLLKAAVSSGIGEETYGPRIMFAGREESPTLDDGISEMDEFFHDSIQKLFDK 120
           NLGLNEYKFLLKAAVSSGIGEETYGPRIMFAGREESPTLDDGISEMDEFFHDSIQKLFDK
Sbjct: 61  NLGLNEYKFLLKAAVSSGIGEETYGPRIMFAGREESPTLDDGISEMDEFFHDSIQKLFDK 120

Query: 121 SGFSPPEIDILVVNVSMLASIPSLSARIINHYKLRPDVKVFNLTGMGCSASLISIDVVSR 180
           SGFSPPEIDILVVNVSMLASIPSLSARIINHYKLRPDVKVFNLTGMGCSASLISIDVVSR
Sbjct: 121 SGFSPPEIDILVVNVSMLASIPSLSARIINHYKLRPDVKVFNLTGMGCSASLISIDVVSR 180

Query: 181 VFKSYKNMNALVITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNNTALKNRAMFKL 240
           VFKSYKNMNALVITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNNTALKNRAMFKL
Sbjct: 181 VFKSYKNMNALVITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNNTALKNRAMFKL 240

Query: 241 KTLVRTHHGARDESYGCCYQTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAPKILPTRE 300
           KTLVRTHHGARDESYGCCYQTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAPKILPTRE
Sbjct: 241 KTLVRTHHGARDESYGCCYQTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAPKILPTRE 300

Query: 301 LLQFLIVTTVKKLKRNAKQGSGVKTSAVNFKTGVDHFCIHTGGKAVIDGIGASLGLEKYD 360
           LLQFLIVTTVKKLKRNAKQGSGVKTSAVNFKTGVDHFCIHTGGKAVIDGIGASLGLEKYD
Sbjct: 301 LLQFLIVTTVKKLKRNAKQGSGVKTSAVNFKTGVDHFCIHTGGKAVIDGIGASLGLEKYD 360

Query: 361 LEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKKGGRVLMISFGAGFKCNSCLWEVVRDL 420
           LEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKKGGRVLMISFGAGFKCNSCLWEVVRDL
Sbjct: 361 LEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKKGGRVLMISFGAGFKCNSCLWEVVRDL 420

Query: 421 EDANVWEDCIQRYPPESLSNPFLEKYGWIQQEDIATFKLPE 462
           EDANVWEDCIQRYPPESLSNPFLEKYGWIQQEDIATFKLPE
Sbjct: 421 EDANVWEDCIQRYPPESLSNPFLEKYGWIQQEDIATFKLPE 461

BLAST of CmoCh20G004730 vs. NCBI nr
Match: KAG7010578.1 (3-ketoacyl-CoA synthase 12, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 934.1 bits (2413), Expect = 4.6e-268
Identity = 460/461 (99.78%), Postives = 460/461 (99.78%), Query Frame = 0

Query: 1   MELLSLFYLGSFLFFLFIIWKHFDEKRHQQCYILEYQCYKPTDDRKLSTEFSGDIIKRTK 60
           MELLSLFYLGSFLFFLFIIWKHFDEKRHQQCYILEYQCYKPTDDRKLSTEFSGDIIKRTK
Sbjct: 1   MELLSLFYLGSFLFFLFIIWKHFDEKRHQQCYILEYQCYKPTDDRKLSTEFSGDIIKRTK 60

Query: 61  NLGLNEYKFLLKAAVSSGIGEETYGPRIMFAGREESPTLDDGISEMDEFFHDSIQKLFDK 120
           NLGLNEYKFLLKAAVSSGIGEETYGPRIMFAGREESPTLDDGISEMDEFFHDSIQKLFDK
Sbjct: 61  NLGLNEYKFLLKAAVSSGIGEETYGPRIMFAGREESPTLDDGISEMDEFFHDSIQKLFDK 120

Query: 121 SGFSPPEIDILVVNVSMLASIPSLSARIINHYKLRPDVKVFNLTGMGCSASLISIDVVSR 180
           SGFSPPEIDILVVNVSMLASIPSLSARIINHYKLRPDVKVFNLTGMGCSASLISIDVVSR
Sbjct: 121 SGFSPPEIDILVVNVSMLASIPSLSARIINHYKLRPDVKVFNLTGMGCSASLISIDVVSR 180

Query: 181 VFKSYKNMNALVITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNNTALKNRAMFKL 240
           VFKSYKNMNALVITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNNTALKNRAMFKL
Sbjct: 181 VFKSYKNMNALVITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNNTALKNRAMFKL 240

Query: 241 KTLVRTHHGARDESYGCCYQTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAPKILPTRE 300
           KTLVRTHHGARDESYGCCYQTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAPKILPTRE
Sbjct: 241 KTLVRTHHGARDESYGCCYQTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAPKILPTRE 300

Query: 301 LLQFLIVTTVKKLKRNAKQGSGVKTSAVNFKTGVDHFCIHTGGKAVIDGIGASLGLEKYD 360
           LLQFLIVTTVKKLKRNAKQGSGVKTSAVNFKTGVDHFCIHTGGKAVIDGIG SLGLEKYD
Sbjct: 301 LLQFLIVTTVKKLKRNAKQGSGVKTSAVNFKTGVDHFCIHTGGKAVIDGIGVSLGLEKYD 360

Query: 361 LEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKKGGRVLMISFGAGFKCNSCLWEVVRDL 420
           LEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKKGGRVLMISFGAGFKCNSCLWEVVRDL
Sbjct: 361 LEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKKGGRVLMISFGAGFKCNSCLWEVVRDL 420

Query: 421 EDANVWEDCIQRYPPESLSNPFLEKYGWIQQEDIATFKLPE 462
           EDANVWEDCIQRYPPESLSNPFLEKYGWIQQEDIATFKLPE
Sbjct: 421 EDANVWEDCIQRYPPESLSNPFLEKYGWIQQEDIATFKLPE 461

BLAST of CmoCh20G004730 vs. NCBI nr
Match: XP_023511960.1 (3-ketoacyl-CoA synthase 12-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 929.5 bits (2401), Expect = 1.1e-266
Identity = 456/461 (98.92%), Postives = 458/461 (99.35%), Query Frame = 0

Query: 1   MELLSLFYLGSFLFFLFIIWKHFDEKRHQQCYILEYQCYKPTDDRKLSTEFSGDIIKRTK 60
           MEL+SLFYLGSFLFFLFIIWKHFDEKRHQQCYILEYQCYKPTDDRKLSTEFSGDIIKRTK
Sbjct: 52  MELMSLFYLGSFLFFLFIIWKHFDEKRHQQCYILEYQCYKPTDDRKLSTEFSGDIIKRTK 111

Query: 61  NLGLNEYKFLLKAAVSSGIGEETYGPRIMFAGREESPTLDDGISEMDEFFHDSIQKLFDK 120
           NLGLNEYKFLLKAAVSSGIGEETYGPRIMFAGREESPTLDDGISEMDEFFHDSIQKLFDK
Sbjct: 112 NLGLNEYKFLLKAAVSSGIGEETYGPRIMFAGREESPTLDDGISEMDEFFHDSIQKLFDK 171

Query: 121 SGFSPPEIDILVVNVSMLASIPSLSARIINHYKLRPDVKVFNLTGMGCSASLISIDVVSR 180
           SGFSPPEIDILVVNVSMLASIPSLSARIINHYKLRPD+KVFNLTGMGCSASLISIDVVSR
Sbjct: 172 SGFSPPEIDILVVNVSMLASIPSLSARIINHYKLRPDIKVFNLTGMGCSASLISIDVVSR 231

Query: 181 VFKSYKNMNALVITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNNTALKNRAMFKL 240
           VFKSYKNMNALVITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNNTALKNRAMFKL
Sbjct: 232 VFKSYKNMNALVITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNNTALKNRAMFKL 291

Query: 241 KTLVRTHHGARDESYGCCYQTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAPKILPTRE 300
           KTLVRTHHGARDESYGCCYQTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAPKILPTRE
Sbjct: 292 KTLVRTHHGARDESYGCCYQTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAPKILPTRE 351

Query: 301 LLQFLIVTTVKKLKRNAKQGSGVKTSAVNFKTGVDHFCIHTGGKAVIDGIGASLGLEKYD 360
           LLQFLIVT VKKLKRN KQGSGVKTSAVNFKTGVDHFCIHTGGKAVIDGIG SLGLEKYD
Sbjct: 352 LLQFLIVTAVKKLKRNGKQGSGVKTSAVNFKTGVDHFCIHTGGKAVIDGIGVSLGLEKYD 411

Query: 361 LEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKKGGRVLMISFGAGFKCNSCLWEVVRDL 420
           LEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKKGGRVLMISFGAGFKCNSCLWEVVRDL
Sbjct: 412 LEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKKGGRVLMISFGAGFKCNSCLWEVVRDL 471

Query: 421 EDANVWEDCIQRYPPESLSNPFLEKYGWIQQEDIATFKLPE 462
           EDANVWEDCIQRYPPESLSNPFLEKYGWIQQEDIATFKLPE
Sbjct: 472 EDANVWEDCIQRYPPESLSNPFLEKYGWIQQEDIATFKLPE 512

BLAST of CmoCh20G004730 vs. NCBI nr
Match: XP_022986863.1 (3-ketoacyl-CoA synthase 12-like [Cucurbita maxima])

HSP 1 Score: 922.2 bits (2382), Expect = 1.8e-264
Identity = 452/461 (98.05%), Postives = 455/461 (98.70%), Query Frame = 0

Query: 1   MELLSLFYLGSFLFFLFIIWKHFDEKRHQQCYILEYQCYKPTDDRKLSTEFSGDIIKRTK 60
           MELLSLFYLGSFLFFLFIIWKHFDEKRHQQCYILEYQCYKPTDDRKLSTEFSGDIIKRTK
Sbjct: 1   MELLSLFYLGSFLFFLFIIWKHFDEKRHQQCYILEYQCYKPTDDRKLSTEFSGDIIKRTK 60

Query: 61  NLGLNEYKFLLKAAVSSGIGEETYGPRIMFAGREESPTLDDGISEMDEFFHDSIQKLFDK 120
           NLGLNEYKF LKAAVSSGIGEETYGPRIMFAGREESPTLDDGISEMDEFFHDSIQKLFDK
Sbjct: 61  NLGLNEYKFFLKAAVSSGIGEETYGPRIMFAGREESPTLDDGISEMDEFFHDSIQKLFDK 120

Query: 121 SGFSPPEIDILVVNVSMLASIPSLSARIINHYKLRPDVKVFNLTGMGCSASLISIDVVSR 180
           SGFSPPEIDILVVNVSMLASIPSLSARIINHYKLRPD+KVFNLTGMGCSASLISIDVVSR
Sbjct: 121 SGFSPPEIDILVVNVSMLASIPSLSARIINHYKLRPDIKVFNLTGMGCSASLISIDVVSR 180

Query: 181 VFKSYKNMNALVITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNNTALKNRAMFKL 240
           VFKSYKN+NALVITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNNT LKNRAMFKL
Sbjct: 181 VFKSYKNVNALVITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNNTTLKNRAMFKL 240

Query: 241 KTLVRTHHGARDESYGCCYQTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAPKILPTRE 300
           KTLVRTHHGA+DESY CCYQTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAPKILPTRE
Sbjct: 241 KTLVRTHHGAKDESYSCCYQTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAPKILPTRE 300

Query: 301 LLQFLIVTTVKKLKRNAKQGSGVKTSAVNFKTGVDHFCIHTGGKAVIDGIGASLGLEKYD 360
           LLQFLIVTTVKKLKRN KQG GVKTSAVNFKTGVDHFCIHTGGKAVIDGIG SLGLEKYD
Sbjct: 301 LLQFLIVTTVKKLKRNGKQGRGVKTSAVNFKTGVDHFCIHTGGKAVIDGIGVSLGLEKYD 360

Query: 361 LEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKKGGRVLMISFGAGFKCNSCLWEVVRDL 420
           LEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKKGGRVLMISFGAGFKCNSCLWEVVRDL
Sbjct: 361 LEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKKGGRVLMISFGAGFKCNSCLWEVVRDL 420

Query: 421 EDANVWEDCIQRYPPESLSNPFLEKYGWIQQEDIATFKLPE 462
           EDANVWEDCIQRYPPESLSNPFLEKYGWIQQEDIATFKLPE
Sbjct: 421 EDANVWEDCIQRYPPESLSNPFLEKYGWIQQEDIATFKLPE 461

BLAST of CmoCh20G004730 vs. NCBI nr
Match: KAG6570735.1 (3-ketoacyl-CoA synthase 12, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 901.4 bits (2328), Expect = 3.3e-258
Identity = 445/446 (99.78%), Postives = 445/446 (99.78%), Query Frame = 0

Query: 1   MELLSLFYLGSFLFFLFIIWKHFDEKRHQQCYILEYQCYKPTDDRKLSTEFSGDIIKRTK 60
           MELLSLFYLGSFLFFLFIIWKHFDEKRHQQCYILEYQCYKPTDDRKLSTEFSGDIIKRTK
Sbjct: 1   MELLSLFYLGSFLFFLFIIWKHFDEKRHQQCYILEYQCYKPTDDRKLSTEFSGDIIKRTK 60

Query: 61  NLGLNEYKFLLKAAVSSGIGEETYGPRIMFAGREESPTLDDGISEMDEFFHDSIQKLFDK 120
           NLGLNEYKFLLKAAVSSGIGEETYGPRIMFAGREESPTLDDGISEMDEFFHDSIQKLFDK
Sbjct: 61  NLGLNEYKFLLKAAVSSGIGEETYGPRIMFAGREESPTLDDGISEMDEFFHDSIQKLFDK 120

Query: 121 SGFSPPEIDILVVNVSMLASIPSLSARIINHYKLRPDVKVFNLTGMGCSASLISIDVVSR 180
           SGFSPPEIDILVVNVSMLASIPSLSARIINHYKLRPDVKVFNLTGMGCSASLISIDVVSR
Sbjct: 121 SGFSPPEIDILVVNVSMLASIPSLSARIINHYKLRPDVKVFNLTGMGCSASLISIDVVSR 180

Query: 181 VFKSYKNMNALVITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNNTALKNRAMFKL 240
           VFKSYKNMNALVITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNNTALKNRAMFKL
Sbjct: 181 VFKSYKNMNALVITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNNTALKNRAMFKL 240

Query: 241 KTLVRTHHGARDESYGCCYQTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAPKILPTRE 300
           KTLVRTHHGARDESYGCCYQTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAPKILPTRE
Sbjct: 241 KTLVRTHHGARDESYGCCYQTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAPKILPTRE 300

Query: 301 LLQFLIVTTVKKLKRNAKQGSGVKTSAVNFKTGVDHFCIHTGGKAVIDGIGASLGLEKYD 360
           LLQFLIVTTVKKLKRNAKQGSGVKTSAVNFKTGVDHFCIHTGGKAVIDGIG SLGLEKYD
Sbjct: 301 LLQFLIVTTVKKLKRNAKQGSGVKTSAVNFKTGVDHFCIHTGGKAVIDGIGVSLGLEKYD 360

Query: 361 LEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKKGGRVLMISFGAGFKCNSCLWEVVRDL 420
           LEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKKGGRVLMISFGAGFKCNSCLWEVVRDL
Sbjct: 361 LEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKKGGRVLMISFGAGFKCNSCLWEVVRDL 420

Query: 421 EDANVWEDCIQRYPPESLSNPFLEKY 447
           EDANVWEDCIQRYPPESLSNPFLEKY
Sbjct: 421 EDANVWEDCIQRYPPESLSNPFLEKY 446

BLAST of CmoCh20G004730 vs. TAIR 10
Match: AT2G28630.1 (3-ketoacyl-CoA synthase 12 )

HSP 1 Score: 665.2 bits (1715), Expect = 3.7e-191
Identity = 325/473 (68.71%), Postives = 387/473 (81.82%), Query Frame = 0

Query: 1   MELLSLFYLGSFLFFLFIIWKHFDEKRHQQCYILEYQCYKPTDDRKLSTEFSGDIIKRTK 60
           M+LL LF+     +  F IWK  D K+ + CYIL+YQC+KPTDDR +ST+FSG+II R +
Sbjct: 1   MDLLFLFFSLLLSYLFFKIWKLIDSKQDKDCYILDYQCHKPTDDRMVSTQFSGEIIYRNQ 60

Query: 61  NLGLNEYKFLLKAAVSSGIGEETYGPRIMFAGREESPTLDDGISEMDEFFHDSIQKLFDK 120
           NLGL EYKFLLKA VSSGIGE+TY PR++F GREE P+L DGISEM+EF+ DSI KL ++
Sbjct: 61  NLGLTEYKFLLKAIVSSGIGEQTYAPRLVFEGREERPSLQDGISEMEEFYVDSIGKLLER 120

Query: 121 SGFSPPEIDILVVNVSMLASIPSLSARIINHYKLRPDVKVFNLTGMGCSASLISIDVVSR 180
           +  SP +IDILVVNVSML+S PSL++RIINHYK+R DVKVFNLTGMGCSASLIS+D+V  
Sbjct: 121 NQISPKDIDILVVNVSMLSSTPSLASRIINHYKMRDDVKVFNLTGMGCSASLISVDIVKN 180

Query: 181 VFKSYKNMNALVITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNNTALKNRAMFKL 240
           +FKSY N  ALV TSESLSPNWYSGN+RSMIL+NCLFRSGGCAILLTN  +L+ +AMFKL
Sbjct: 181 IFKSYANKLALVATSESLSPNWYSGNNRSMILANCLFRSGGCAILLTNKRSLRKKAMFKL 240

Query: 241 KTLVRTHHGARDESYGCCYQTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAPKILPTRE 300
           K +VRTHHGAR+ESY CC Q ED++G +GF+LGKNLPKAATRAFV+NL+ I PKILP  E
Sbjct: 241 KCMVRTHHGAREESYNCCIQAEDEQGRVGFYLGKNLPKAATRAFVENLKVITPKILPVTE 300

Query: 301 LLQFLIVTTVKKLK--RNAKQGS-----GVKTSA-VNFKTGVDHFCIHTGGKAVIDGIGA 360
           L++F++   +KK+K  +N  +GS     G    A +NFKTG++HFCIHTGGKAVIDGIG 
Sbjct: 301 LIRFMLKLLIKKIKIRQNPSKGSTNLPPGTPLKAGINFKTGIEHFCIHTGGKAVIDGIGH 360

Query: 361 SLGLEKYDLEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKKGGRVLMISFGAGFKCNSC 420
           SL L +YD+EPARMTLHRFGNTSASSLWYVLAYMEAKKRLK+G RV MISFGAGFKCNSC
Sbjct: 361 SLDLNEYDIEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKRGDRVFMISFGAGFKCNSC 420

Query: 421 LWEVVRDL----EDANVWEDCIQRYPPESLSNPFLEKYGWIQQEDIATFKLPE 462
           +WEVVRDL       NVW  CI  YPP+S+ NP+LEK+GWIQ ED  TFK+P+
Sbjct: 421 VWEVVRDLTGGESKGNVWNHCIDDYPPKSILNPYLEKFGWIQDEDPDTFKVPD 473

BLAST of CmoCh20G004730 vs. TAIR 10
Match: AT1G07720.1 (3-ketoacyl-CoA synthase 3 )

HSP 1 Score: 652.5 bits (1682), Expect = 2.5e-187
Identity = 320/476 (67.23%), Postives = 380/476 (79.83%), Query Frame = 0

Query: 1   MELLSLFYLGSFLFFLFIIWKHFDEKRHQQCYILEYQCYKPTDDRKLSTEFSGDIIKRTK 60
           M+LL +       + +F IWK  D KR Q CYIL+YQC+KP+DDR ++T+FSGDII R K
Sbjct: 1   MDLLVMLLSLLVSYLIFKIWKRIDSKRDQNCYILDYQCHKPSDDRMVNTQFSGDIILRNK 60

Query: 61  NLGLNEYKFLLKAAVSSGIGEETYGPRIMFAGREESPTLDDGISEMDEFFHDSIQKLFDK 120
           +L LNEYKFLLKA VSSGIGE+TY PR+ F GRE+ PTL DG+SEM+EF+ D+I+K+  +
Sbjct: 61  HLRLNEYKFLLKAIVSSGIGEQTYAPRLFFEGREQRPTLQDGLSEMEEFYIDTIEKVLKR 120

Query: 121 SGFSPPEIDILVVNVSMLASIPSLSARIINHYKLRPDVKVFNLTGMGCSASLISIDVVSR 180
           +  SP EIDILVVNVSML S PSLSARIINHYK+R D+KVFNLT MGCSAS+ISID+V  
Sbjct: 121 NKISPSEIDILVVNVSMLNSTPSLSARIINHYKMREDIKVFNLTAMGCSASVISIDIVKN 180

Query: 181 VFKSYKNMNALVITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNNTALKNRAMFKL 240
           +FK+YKN  ALV+TSESLSPNWYSGN+RSMIL+NCLFRSGGCA+LLTN  +L  RAMFKL
Sbjct: 181 IFKTYKNKLALVVTSESLSPNWYSGNNRSMILANCLFRSGGCAVLLTNKRSLSRRAMFKL 240

Query: 241 KTLVRTHHGARDESYGCCYQTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAPKILPTRE 300
           + LVRTHHGARD+S+  C Q ED+ G++G HL K LPKAATRAF+DNL+ I PKILP  E
Sbjct: 241 RCLVRTHHGARDDSFNACVQKEDELGHIGVHLDKTLPKAATRAFIDNLKVITPKILPVTE 300

Query: 301 LLQFLIVTTVKKLKRNAKQGS----------GVKTSAVNFKTGVDHFCIHTGGKAVIDGI 360
           LL+F++   +KKL+ +  +GS          GVK + +NFKTG+DHFCIHTGGKAVID I
Sbjct: 301 LLRFMLCLLLKKLRSSPSKGSTNVTQAAPKAGVK-AGINFKTGIDHFCIHTGGKAVIDAI 360

Query: 361 GASLGLEKYDLEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKKGGRVLMISFGAGFKCN 420
           G SL L +YDLEPARMTLHRFGNTSASSLWYVL YMEAKKRLK+G RV MISFGAGFKCN
Sbjct: 361 GYSLDLNEYDLEPARMTLHRFGNTSASSLWYVLGYMEAKKRLKRGDRVFMISFGAGFKCN 420

Query: 421 SCLWEVVRDLE----DANVWEDCIQRYPPESLSNPFLEKYGWI-QQEDIATFKLPE 462
           SC+WEVVRDL       NVW  CI +YPP+S+ NPF EKYGWI ++ED  TFK+PE
Sbjct: 421 SCVWEVVRDLNVGEAVGNVWNHCINQYPPKSILNPFFEKYGWIHEEEDPDTFKMPE 475

BLAST of CmoCh20G004730 vs. TAIR 10
Match: AT5G04530.1 (3-ketoacyl-CoA synthase 19 )

HSP 1 Score: 515.4 bits (1326), Expect = 4.8e-146
Identity = 249/460 (54.13%), Postives = 340/460 (73.91%), Query Frame = 0

Query: 4   LSLFYLGSFL----FFLFIIWKHFDEKRHQQ-CYILEYQCYKPTDDRKLSTEFSGDIIKR 63
           + LF L S L     F+F I+K   ++R+Q+ CY+L Y+CYK  ++RKL TE    +++R
Sbjct: 1   MELFSLSSLLLLSTLFVFYIFKFVFKRRNQRNCYMLHYECYKGMEERKLDTETCAKVVQR 60

Query: 64  TKNLGLNEYKFLLKAAVSSGIGEETYGPRIMFAGREESPTLDDGISEMDEFFHDSIQKLF 123
            KNLGL EY+FLL+   SSGIGEETYGPR +  GRE+SPTL D  SEMDE   D++ KLF
Sbjct: 61  NKNLGLEEYRFLLRTMASSGIGEETYGPRNVLEGREDSPTLLDAHSEMDEIMFDTLDKLF 120

Query: 124 DKS--GFSPPEIDILVVNVSMLASIPSLSARIINHYKLRPDVKVFNLTGMGCSASLISID 183
            K+    SP +IDILVVNVS+ A  PSL++R+IN YK+R D+K +NL+G+GCSAS+ISID
Sbjct: 121 HKTKGSISPSDIDILVVNVSLFAPSPSLTSRVINRYKMREDIKSYNLSGLGCSASVISID 180

Query: 184 VVSRVFKSYKNMNALVITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNNTALKNRA 243
           +V R+F++ +N  ALV+++E++ P+WY G DRSM+LSNCLFR+GG ++LLTN    KN+A
Sbjct: 181 IVQRMFETRENALALVVSTETMGPHWYCGKDRSMMLSNCLFRAGGSSVLLTNAARFKNQA 240

Query: 244 MFKLKTLVRTHHGARDESYGCCYQTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAPKIL 303
           + KL T+VR H G+ DE+Y CC Q ED  G+ GF L K L KAA RA   NL+ + P++L
Sbjct: 241 LMKLVTVVRAHVGSDDEAYSCCIQMEDRDGHPGFLLTKYLKKAAARALTKNLQVLLPRVL 300

Query: 304 PTRELLQFLIVTTVKKLKRNAKQGSGVKTSAVNFKTGVDHFCIHTGGKAVIDGIGASLGL 363
           P +EL+++ IV  +K+ + +AK+        +N KTG+ HFCIH GG+A+I+G+G SLGL
Sbjct: 301 PVKELIRYAIVRALKR-RTSAKREPASSGIGLNLKTGLQHFCIHPGGRAIIEGVGKSLGL 360

Query: 364 EKYDLEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKKGGRVLMISFGAGFKCNSCLWEV 423
            ++D+EPARM LHRFGNTS+  LWYVL YMEAK RLKKG ++LM+S GAGF+ N+C+WEV
Sbjct: 361 TEFDIEPARMALHRFGNTSSGGLWYVLGYMEAKNRLKKGEKILMMSMGAGFESNNCVWEV 420

Query: 424 VRDLEDANVWEDCIQRYPPES-LSNPFLEKYGWIQQEDIA 456
           ++DL+D NVWED + RYP  S + NPF+EKY WI  + ++
Sbjct: 421 LKDLDDKNVWEDSVDRYPELSRIPNPFVEKYDWINDDTMS 459

BLAST of CmoCh20G004730 vs. TAIR 10
Match: AT1G01120.1 (3-ketoacyl-CoA synthase 1 )

HSP 1 Score: 347.1 bits (889), Expect = 2.2e-95
Identity = 175/429 (40.79%), Postives = 264/429 (61.54%), Query Frame = 0

Query: 13  LFFLFIIWKHFDEKRHQQCYILEYQCYKPTDDRKLSTEFSGDIIKRTKNLGLNEYKFLLK 72
           L FL  +   +   R +  Y++++ CYKP D+RK+S +    + +   +   +  +F  +
Sbjct: 104 LVFLSFVLTLYVANRSKPVYLVDFSCYKPEDERKISVDSFLTMTEENGSFTDDTVQFQQR 163

Query: 73  AAVSSGIGEETYGPRIMFAGREESP---TLDDGISEMDEFFHDSIQKLFDKSGFSPPEID 132
            +  +G+G+ETY PR    G   +P    + +  +E +     ++  LF+K+G  P E+ 
Sbjct: 164 ISNRAGLGDETYLPR----GITSTPPKLNMSEARAEAEAVMFGALDSLFEKTGIKPAEVG 223

Query: 133 ILVVNVSMLASIPSLSARIINHYKLRPDVKVFNLTGMGCSASLISIDVVSRVFKSYKNMN 192
           IL+VN S+    PSLSA I+NHYK+R D+K +NL GMGCSA LISID+ + + K+  N  
Sbjct: 224 ILIVNCSLFNPTPSLSAMIVNHYKMREDIKSYNLGGMGCSAGLISIDLANNLLKANPNSY 283

Query: 193 ALVITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNNTALKNRAMFKLKTLVRTHHG 252
           A+V+++E+++ NWY GNDRSM+L NC+FR GG AILL+N    + ++ + L  +VRTH G
Sbjct: 284 AVVVSTENITLNWYFGNDRSMLLCNCIFRMGGAAILLSNRRQDRKKSKYSLVNVVRTHKG 343

Query: 253 ARDESYGCCYQTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAPKILPTRELLQFLIVTT 312
           + D++Y C YQ ED++G +G  L + L   A  A   N+  + P +LP  E L FLI   
Sbjct: 344 SDDKNYNCVYQKEDERGTIGVSLARELMSVAGDALKTNITTLGPMVLPLSEQLMFLISLV 403

Query: 313 VKKLKRNAKQGSGVKTSAVNFKTGVDHFCIHTGGKAVIDGIGASLGLEKYDLEPARMTLH 372
            +K+ +       VK    +FK   +HFCIH GG+AV+D +  +L L+ + +EP+RMTLH
Sbjct: 404 KRKMFK-----LKVKPYIPDFKLAFEHFCIHAGGRAVLDEVQKNLDLKDWHMEPSRMTLH 463

Query: 373 RFGNTSASSLWYVLAYMEAKKRLKKGGRVLMISFGAGFKCNSCLWEVVRDLE----DANV 432
           RFGNTS+SSLWY +AY EAK R+K G R+  I+FG+GFKCNS +W+ +R +       N 
Sbjct: 464 RFGNTSSSSLWYEMAYTEAKGRVKAGDRLWQIAFGSGFKCNSAVWKALRPVSTEEMTGNA 523

Query: 433 WEDCIQRYP 435
           W   I +YP
Sbjct: 524 WAGSIDQYP 523

BLAST of CmoCh20G004730 vs. TAIR 10
Match: AT2G46720.1 (3-ketoacyl-CoA synthase 13 )

HSP 1 Score: 336.7 bits (862), Expect = 3.0e-92
Identity = 169/427 (39.58%), Postives = 260/427 (60.89%), Query Frame = 0

Query: 13  LFFLFIIWKHFDEKRHQQCYILEYQCYKPTDDRKLSTEFSGDIIKRTKNLGLNEYKFLLK 72
           L  + I +  +   R +  Y++++ C++PTD  K+S+E   ++ K  +       +F+ +
Sbjct: 42  LLLISIFFYAYSTTRSKPVYLVDFSCHQPTDSCKISSETFFNMAKGAQLYTDETIQFMTR 101

Query: 73  AAVSSGIGEETYGPRIMFAGREESPTLDDGISEMDEFFHDSIQKLFDKSGFSPPEIDILV 132
               SG+G++TY PR M      +P++ +   E +     ++  LF K+G  P E+ I +
Sbjct: 102 ILNRSGLGDDTYSPRCMLTS-PPTPSMYEARHESELVIFGALNSLFKKTGIEPREVGIFI 161

Query: 133 VNVSMLASIPSLSARIINHYKLRPDVKVFNLTGMGCSASLISIDVVSRVFKSYKNMNALV 192
           VN S+    PSLS+ I+N YKL+ DVK +NL+GMGCSA  IS+D+ + + K+  N  A++
Sbjct: 162 VNCSLFNPNPSLSSMIVNRYKLKTDVKTYNLSGMGCSAGAISVDLATNLLKANPNTYAVI 221

Query: 193 ITSESLSPNWYSGNDRSMILSNCLFRSGGCAILLTNNTALKNRAMFKLKTLVRTHHGARD 252
           +++E+++ + Y GNDRSM++ NCLFR GG A++L+N +  + R+ ++L  +VRTH G+ D
Sbjct: 222 VSTENMTLSMYRGNDRSMLVPNCLFRVGGAAVMLSNRSQDRVRSKYELTHIVRTHKGSSD 281

Query: 253 ESYGCCYQTEDDKGYMGFHLGKNLPKAATRAFVDNLREIAPKILPTRELLQFLIVTTVKK 312
           + Y C  Q ED KG +G  L K L   A  +   NL  + P +LP  E L+F++     K
Sbjct: 282 KHYTCAEQKEDSKGIVGVALSKELTVVAGDSLKTNLTALGPLVLPLSEKLRFILFLVKSK 341

Query: 313 LKRNAKQGSGVKTSAVNFKTGVDHFCIHTGGKAVIDGIGASLGLEKYDLEPARMTLHRFG 372
           L R       V     +FK    HFCIH GG+A++D +   LGL ++DLEP+RMTLHRFG
Sbjct: 342 LFR-----LKVSPYVPDFKLCFKHFCIHAGGRALLDAVEKGLGLSEFDLEPSRMTLHRFG 401

Query: 373 NTSASSLWYVLAYMEAKKRLKKGGRVLMISFGAGFKCNSCLWEVVRDLED-----ANVWE 432
           NTS+SSLWY LAY+EAK R+K+G RV  ++FG+GFKCNS +W  +R +        N W 
Sbjct: 402 NTSSSSLWYELAYVEAKCRVKRGDRVWQLAFGSGFKCNSIVWRALRTIPANESLVGNPWG 461

Query: 433 DCIQRYP 435
           D + +YP
Sbjct: 462 DSVHKYP 462

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SIB25.3e-19068.713-ketoacyl-CoA synthase 12 OS=Arabidopsis thaliana OX=3702 GN=KCS12 PE=2 SV=1[more]
Q9LQP83.5e-18667.233-ketoacyl-CoA synthase 3 OS=Arabidopsis thaliana OX=3702 GN=KCS3 PE=2 SV=3[more]
Q9LZ726.7e-14554.133-ketoacyl-CoA synthase 19 OS=Arabidopsis thaliana OX=3702 GN=KCS19 PE=2 SV=1[more]
Q9MAM33.2e-9440.793-ketoacyl-CoA synthase 1 OS=Arabidopsis thaliana OX=3702 GN=KCS1 PE=1 SV=1[more]
Q9ZUZ04.3e-9139.583-ketoacyl-CoA synthase 13 OS=Arabidopsis thaliana OX=3702 GN=HIC PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FSZ57.7e-269100.003-ketoacyl-CoA synthase OS=Cucurbita moschata OX=3662 GN=LOC111448188 PE=3 SV=1[more]
A0A6J1JHS68.8e-26598.053-ketoacyl-CoA synthase OS=Cucurbita maxima OX=3661 GN=LOC111484475 PE=3 SV=1[more]
A0A5A7V8E04.7e-24289.153-ketoacyl-CoA synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3BJH04.7e-24289.153-ketoacyl-CoA synthase OS=Cucumis melo OX=3656 GN=LOC103490572 PE=3 SV=1[more]
A0A6J1KYJ76.8e-24188.073-ketoacyl-CoA synthase OS=Cucurbita maxima OX=3661 GN=LOC111499896 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022943417.11.6e-268100.003-ketoacyl-CoA synthase 12-like [Cucurbita moschata][more]
KAG7010578.14.6e-26899.783-ketoacyl-CoA synthase 12, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023511960.11.1e-26698.923-ketoacyl-CoA synthase 12-like [Cucurbita pepo subsp. pepo][more]
XP_022986863.11.8e-26498.053-ketoacyl-CoA synthase 12-like [Cucurbita maxima][more]
KAG6570735.13.3e-25899.783-ketoacyl-CoA synthase 12, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT2G28630.13.7e-19168.713-ketoacyl-CoA synthase 12 [more]
AT1G07720.12.5e-18767.233-ketoacyl-CoA synthase 3 [more]
AT5G04530.14.8e-14654.133-ketoacyl-CoA synthase 19 [more]
AT1G01120.12.2e-9540.793-ketoacyl-CoA synthase 1 [more]
AT2G46720.13.0e-9239.583-ketoacyl-CoA synthase 13 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016039Thiolase-likeGENE3D3.40.47.10coord: 64..416
e-value: 1.9E-70
score: 239.8
IPR016039Thiolase-likeSUPERFAMILY53901Thiolase-likecoord: 97..417
IPR0137473-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III, C-terminalPFAMPF08541ACP_syn_III_Ccoord: 334..415
e-value: 2.4E-13
score: 50.1
IPR013601FAE1/Type III polyketide synthase-like proteinPFAMPF08392FAE1_CUT1_RppAcoord: 25..312
e-value: 1.3E-113
score: 379.1
IPR012392Very-long-chain 3-ketoacyl-CoA synthasePIRSFPIRSF036417Very_long_ch_3-ktacl-CoA_syncoord: 2..448
e-value: 1.3E-173
score: 575.9
IPR012392Very-long-chain 3-ketoacyl-CoA synthasePANTHERPTHR315613-KETOACYL-COA SYNTHASEcoord: 1..453
NoneNo IPR availablePANTHERPTHR31561:SF243-KETOACYL-COA SYNTHASE 12coord: 1..453
NoneNo IPR availableCDDcd00831CHS_likecoord: 15..414
e-value: 2.15418E-120
score: 354.223

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh20G004730.1CmoCh20G004730.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006633 fatty acid biosynthetic process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0102756 very-long-chain 3-ketoacyl-CoA synthase activity
molecular_function GO:0016746 acyltransferase activity
molecular_function GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups