CmaCh03G006310 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh03G006310
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionendochitinase-like
LocationCma_Chr03: 5447973 .. 5449529 (+)
RNA-Seq ExpressionCmaCh03G006310
SyntenyCmaCh03G006310
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGCTCCGTACGCTTATTGTCTTAGCCTTTGCCTTCGTTTTGGGAGCCGCAGCGGAGCAATGTGGACGGCAGGCCAACGGCGCTCTCTGCCCCAATGGCCTCTGCTGCAGCCAGTTCGGATGGTGCGGCGACACCGACGAGTACTGTAAAAATGGTTGCCAGAGCCAGTGTGGTGGCACGACTCCTTCCACACCCCCCAGTAGCGGCGGCGGTGGCGTCGGAAGTATCATCAGCGAAGCCCTCTACAATCAAATGCTCAAGTATAGCAAGGATCCTCGATGCCCCAGTAATGGATTCTATCAATACAATGCTTTCATTGCTGCTACTCAATCCTTCCCTGGCTTCGGTACCACCGGAGATGCCGCTACTCGTAAAAGGGAGCTCGCAGCTTTCTTCGGCCAAACTTCTCATGAAACTACTGGTTAGTGTTACATTTCCTAAATTAGTCAACGTGGGACTCTTCCTCCAACAATGTTTAACAATCCTTCCCTCGAATAAAGTAGATTATAGAGTCTCTTTTGAGGTCTATGGAGCCCTCGAACAACCTCCCCTTAATCGAGCCCTCGAACAAACTCTTTCTCTAGAGCCTTCGAACAAAATAGACTATAGAGCCTCTCTTGAGGTCTATGGAGCCCTTGAACACCCTCCCCTTAATTGAGCCTCAACTCCTTCACTAGAACCTTTGAACAAAGTACACATTTTGTTCGACCTTTTTGACTGCACCTTCGAGGGCATGACTCTGATACCATGTTAGGAACCACTAACCTATATAATGATATGATATTGTCCACTTTAAGTATAAGTTCTTAGCATTATTTTGGGTTTCTCGAATGTTTCATTCTAATTGAGATATATTTCTTTATAAACTCATGCTCAAGATCCCATGATGTTTGTTGGAAAAATTGTCATTTTGATTTACAAATGAATATTAAAGCGAGTGTGGCGCAGGAGGGTGGGCTACCGCACCGGATGGTCCATATGCATGGGGATATTGCTTCATAAGGGAGATTAACCAAGACGTATATTGCTCACCGAACCAGCAATGGCCATGCGCTGCTGGCCAGAAATATTACGGCCGTGGACCAATTCAGCTAACCCAGTAAGACCTCTGTTCTTCCTATAACACAACATATCATCAAACTTCTGAGATTCTAATCCTAATTCGTTAAATTATCAAACTTTTCTAGTAACTACAACTACGGGCCAGCGGGTAATGCACTAAACTTAAATCTGCTAAGCAACCCTGATTTGGTAGCCACGGATGCGGTTGTTTCATTCAAGACAGCCATTTGGTTTTGGATGACACCACAAGGAAACAAGCCATCGTGCCATGATGTTATCACTGGGAGATGGCAGCCTTCGAGCGCCGACAGCGCTGCCGGAAGAGTGCCTGGCTATGGTGTCATTACCAACATCATTAATGGTGGGCTCGAGTGCGGACATGGCCCCGATGATAGAGTTGCTGATAGAATTGGGTTCTACAAAAGATACTCCGACATGCTTGGTGTCAGTTACGGCAGCAACTTAGATTGCAACAATCAAAGGTCTTTCTAA

mRNA sequence

ATGAAGCTCCGTACGCTTATTGTCTTAGCCTTTGCCTTCGTTTTGGGAGCCGCAGCGGAGCAATGTGGACGGCAGGCCAACGGCGCTCTCTGCCCCAATGGCCTCTGCTGCAGCCAGTTCGGATGGTGCGGCGACACCGACGAGTACTGTAAAAATGGTTGCCAGAGCCAGTGTGGTGGCACGACTCCTTCCACACCCCCCAGTAGCGGCGGCGGTGGCGTCGGAAGTATCATCAGCGAAGCCCTCTACAATCAAATGCTCAAGTATAGCAAGGATCCTCGATGCCCCAGTAATGGATTCTATCAATACAATGCTTTCATTGCTGCTACTCAATCCTTCCCTGGCTTCGGTACCACCGGAGATGCCGCTACTCGTAAAAGGGAGCTCGCAGCTTTCTTCGGCCAAACTTCTCATGAAACTACTGGAGGGTGGGCTACCGCACCGGATGGTCCATATGCATGGGGATATTGCTTCATAAGGGAGATTAACCAAGACGTATATTGCTCACCGAACCAGCAATGGCCATGCGCTGCTGGCCAGAAATATTACGGCCGTGGACCAATTCAGCTAACCCATAACTACAACTACGGGCCAGCGGGTAATGCACTAAACTTAAATCTGCTAAGCAACCCTGATTTGGTAGCCACGGATGCGGTTGTTTCATTCAAGACAGCCATTTGGTTTTGGATGACACCACAAGGAAACAAGCCATCGTGCCATGATGTTATCACTGGGAGATGGCAGCCTTCGAGCGCCGACAGCGCTGCCGGAAGAGTGCCTGGCTATGGTGTCATTACCAACATCATTAATGGTGGGCTCGAGTGCGGACATGGCCCCGATGATAGAGTTGCTGATAGAATTGGGTTCTACAAAAGATACTCCGACATGCTTGGTGTCAGTTACGGCAGCAACTTAGATTGCAACAATCAAAGGTCTTTCTAA

Coding sequence (CDS)

ATGAAGCTCCGTACGCTTATTGTCTTAGCCTTTGCCTTCGTTTTGGGAGCCGCAGCGGAGCAATGTGGACGGCAGGCCAACGGCGCTCTCTGCCCCAATGGCCTCTGCTGCAGCCAGTTCGGATGGTGCGGCGACACCGACGAGTACTGTAAAAATGGTTGCCAGAGCCAGTGTGGTGGCACGACTCCTTCCACACCCCCCAGTAGCGGCGGCGGTGGCGTCGGAAGTATCATCAGCGAAGCCCTCTACAATCAAATGCTCAAGTATAGCAAGGATCCTCGATGCCCCAGTAATGGATTCTATCAATACAATGCTTTCATTGCTGCTACTCAATCCTTCCCTGGCTTCGGTACCACCGGAGATGCCGCTACTCGTAAAAGGGAGCTCGCAGCTTTCTTCGGCCAAACTTCTCATGAAACTACTGGAGGGTGGGCTACCGCACCGGATGGTCCATATGCATGGGGATATTGCTTCATAAGGGAGATTAACCAAGACGTATATTGCTCACCGAACCAGCAATGGCCATGCGCTGCTGGCCAGAAATATTACGGCCGTGGACCAATTCAGCTAACCCATAACTACAACTACGGGCCAGCGGGTAATGCACTAAACTTAAATCTGCTAAGCAACCCTGATTTGGTAGCCACGGATGCGGTTGTTTCATTCAAGACAGCCATTTGGTTTTGGATGACACCACAAGGAAACAAGCCATCGTGCCATGATGTTATCACTGGGAGATGGCAGCCTTCGAGCGCCGACAGCGCTGCCGGAAGAGTGCCTGGCTATGGTGTCATTACCAACATCATTAATGGTGGGCTCGAGTGCGGACATGGCCCCGATGATAGAGTTGCTGATAGAATTGGGTTCTACAAAAGATACTCCGACATGCTTGGTGTCAGTTACGGCAGCAACTTAGATTGCAACAATCAAAGGTCTTTCTAA

Protein sequence

MKLRTLIVLAFAFVLGAAAEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKNGCQSQCGGTTPSTPPSSGGGGVGSIISEALYNQMLKYSKDPRCPSNGFYQYNAFIAATQSFPGFGTTGDAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIREINQDVYCSPNQQWPCAAGQKYYGRGPIQLTHNYNYGPAGNALNLNLLSNPDLVATDAVVSFKTAIWFWMTPQGNKPSCHDVITGRWQPSSADSAAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDMLGVSYGSNLDCNNQRSF
Homology
BLAST of CmaCh03G006310 vs. ExPASy Swiss-Prot
Match: P93680 (Endochitinase OS=Persea americana OX=3435 GN=chi1 PE=1 SV=2)

HSP 1 Score: 477.6 bits (1228), Expect = 1.1e-133
Identity = 220/313 (70.29%), Postives = 252/313 (80.51%), Query Frame = 0

Query: 3   LRTLIVLAFAFVLGAA-AEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKNGCQSQCGGT 62
           L  L++L    + G A AEQCGRQA GALCP GLCCSQFGWCG T +YC   CQSQCGG 
Sbjct: 8   LPLLLLLLVGLLAGEAFAEQCGRQAGGALCPGGLCCSQFGWCGSTSDYCGPTCQSQCGGV 67

Query: 63  TPSTPPSSGGGGVGSIISEALYNQMLKYSKDPRCPSNGFYQYNAFIAATQSFPGFGTTGD 122
           TPS      GGGV S+IS++++NQMLK+  D  C + GFY YNAFIAA  SF GF + GD
Sbjct: 68  TPSP-----GGGVASLISQSVFNQMLKHRNDAACQAKGFYTYNAFIAAANSFNGFASVGD 127

Query: 123 AATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIREI-NQDVYCSPNQQWPCAAGQ 182
            ATRKRE+AAF  QTSHETTGGWATAPDGPYAWGYCF++E  N   YC P  QWPCA G+
Sbjct: 128 TATRKREIAAFLAQTSHETTGGWATAPDGPYAWGYCFLKEQGNPPDYCVPTAQWPCAPGK 187

Query: 183 KYYGRGPIQLTHNYNYGPAGNALNLNLLSNPDLVATDAVVSFKTAIWFWMTPQGNKPSCH 242
           KYYGRGPIQ+++NYNYGPAG A+  +L++NPD VATD V+SFKTA+WFWMTPQ  KPSCH
Sbjct: 188 KYYGRGPIQISYNYNYGPAGRAIGYDLINNPDAVATDPVISFKTALWFWMTPQSPKPSCH 247

Query: 243 DVITGRWQPSSADSAAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDMLGVS 302
           +VITGRW PS+AD AAGR+PGYGVITNIINGG+ECG G +D+VADRIGFYKRY D+LGVS
Sbjct: 248 NVITGRWTPSAADRAAGRLPGYGVITNIINGGIECGKGFNDKVADRIGFYKRYCDLLGVS 307

Query: 303 YGSNLDCNNQRSF 314
           YGSNLDC NQRSF
Sbjct: 308 YGSNLDCYNQRSF 315

BLAST of CmaCh03G006310 vs. ExPASy Swiss-Prot
Match: Q42993 (Chitinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht1 PE=2 SV=1)

HSP 1 Score: 473.8 bits (1218), Expect = 1.5e-132
Identity = 220/321 (68.54%), Postives = 253/321 (78.82%), Query Frame = 0

Query: 1   MKLRTLIVLAFAF-VLGAAAEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKNGCQSQCG 60
           M+   ++V+A AF V+    EQCG QA GALCPN LCCSQ+GWCG T  YC +GCQSQC 
Sbjct: 1   MRALAVVVVATAFAVVAVRGEQCGSQAGGALCPNCLCCSQYGWCGSTSAYCGSGCQSQCS 60

Query: 61  GTTPS---TPPSSGGG-GVGSIISEALYNQMLKYSKDPRCPSNGFYQYNAFIAATQSFPG 120
           G+      TPPS GGG GV SI+S +L++QML +  D  CP+  FY Y+AF+AA  +FP 
Sbjct: 61  GSCGGGGPTPPSGGGGSGVASIVSRSLFDQMLLHRNDAACPAKNFYTYDAFVAAANAFPS 120

Query: 121 FGTTGDAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIREINQDV---YCSPNQ 180
           F TTGDAATRKRE+AAF  QTSHETTGGWATAPDGPY+WGYCF  E N +V   YC  + 
Sbjct: 121 FATTGDAATRKREVAAFLAQTSHETTGGWATAPDGPYSWGYCFKEENNGNVGSDYCVQSS 180

Query: 181 QWPCAAGQKYYGRGPIQLTHNYNYGPAGNALNLNLLSNPDLVATDAVVSFKTAIWFWMTP 240
           QWPCAAG+KYYGRGPIQ+++NYNYGPAG A+  NLLSNPDLVA+DA VSFKTA WFWMTP
Sbjct: 181 QWPCAAGKKYYGRGPIQISYNYNYGPAGQAIGSNLLSNPDLVASDATVSFKTAFWFWMTP 240

Query: 241 QGNKPSCHDVITGRWQPSSADSAAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKR 300
           Q  KPSCH V+TG+W P+  D AAGRVPGYGV+TNIINGG+ECGHG D RVADRIGFYKR
Sbjct: 241 QSPKPSCHAVMTGQWTPNGNDQAAGRVPGYGVVTNIINGGVECGHGADSRVADRIGFYKR 300

Query: 301 YSDMLGVSYGSNLDCNNQRSF 314
           Y DMLGVSYG+NLDC NQR F
Sbjct: 301 YCDMLGVSYGANLDCYNQRPF 321

BLAST of CmaCh03G006310 vs. ExPASy Swiss-Prot
Match: P36907 (Endochitinase OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 472.2 bits (1214), Expect = 4.4e-132
Identity = 218/316 (68.99%), Postives = 255/316 (80.70%), Query Frame = 0

Query: 1   MKLRTLIVLAFAFVLGAAAEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKNGCQSQC-G 60
           +K+   I+      LG+ AEQCG QA GA+CPNGLCCS+FG+CG TD YC +GCQSQC  
Sbjct: 5   LKVSFFILCLLPLFLGSKAEQCGSQAGGAVCPNGLCCSKFGFCGSTDPYCGDGCQSQCKS 64

Query: 61  GTTPSTP-PSSGGGGVGSIISEALYNQMLKYSKDPRCPSNGFYQYNAFIAATQSFPGFGT 120
             TP+ P PS+GGG VG ++  +L++QMLKY  D RC  +GFY Y+AFIAA +SF GFGT
Sbjct: 65  SPTPTIPTPSTGGGDVGRLVPSSLFDQMLKYRNDGRCAGHGFYTYDAFIAAARSFNGFGT 124

Query: 121 TGDAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIREIN-QDVYCSPNQQWPCA 180
           TGD  T+K+ELAAF  QTSHETTGGW TAPDGPYAWGYCF+ E N Q+VYCSP + WPCA
Sbjct: 125 TGDDNTKKKELAAFLAQTSHETTGGWPTAPDGPYAWGYCFVSEQNTQEVYCSP-KDWPCA 184

Query: 181 AGQKYYGRGPIQLTHNYNYGPAGNALNLNLLSNPDLVATDAVVSFKTAIWFWMTPQGNKP 240
            G+KYYGRGPIQLTHNYNYG AG A+  +L++NPDL++T+  VSFKTAIWFWMTPQ NKP
Sbjct: 185 PGKKYYGRGPIQLTHNYNYGLAGQAIKEDLINNPDLLSTNPTVSFKTAIWFWMTPQANKP 244

Query: 241 SCHDVITGRWQPSSADSAAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDML 300
           S HDVITGRW PS+ADS+AGRVPGYGVITNIINGG+ECGHG D+RV DR+GFYKRY  + 
Sbjct: 245 SSHDVITGRWTPSAADSSAGRVPGYGVITNIINGGIECGHGQDNRVDDRVGFYKRYCQIF 304

Query: 301 GVSYGSNLDCNNQRSF 314
           GV  G NLDCNNQRSF
Sbjct: 305 GVDPGGNLDCNNQRSF 319

BLAST of CmaCh03G006310 vs. ExPASy Swiss-Prot
Match: P08252 (Endochitinase A OS=Nicotiana tabacum OX=4097 GN=CHN48 PE=1 SV=2)

HSP 1 Score: 466.5 bits (1199), Expect = 2.4e-130
Identity = 214/316 (67.72%), Postives = 253/316 (80.06%), Query Frame = 0

Query: 2   KLRTLIVLAFA-FVLGAAAEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKNG-CQSQC- 61
           K   L  L F+  +L A+AEQCG QA GA CP+GLCCS+FGWCG+T++YC  G CQSQC 
Sbjct: 5   KFTALSSLLFSLLLLSASAEQCGSQAGGARCPSGLCCSKFGWCGNTNDYCGPGNCQSQCP 64

Query: 62  GGTTPSTPPSSGGGGVGSIISEALYNQMLKYSKDPRCPSNGFYQYNAFIAATQSFPGFGT 121
           GG TP+ P   GGG +GSIIS ++++QMLK+  D  C   GFY YNAFI A +SFPGFGT
Sbjct: 65  GGPTPTPPTPPGGGDLGSIISSSMFDQMLKHRNDNACQGKGFYSYNAFINAARSFPGFGT 124

Query: 122 TGDAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIREI-NQDVYCSPNQQWPCA 181
           +GD   RKRE+AAFF QTSHETTGGWATAPDGPYAWGYC++RE  +   YC+P+ QWPCA
Sbjct: 125 SGDTTARKREIAAFFAQTSHETTGGWATAPDGPYAWGYCWLREQGSPGDYCTPSGQWPCA 184

Query: 182 AGQKYYGRGPIQLTHNYNYGPAGNALNLNLLSNPDLVATDAVVSFKTAIWFWMTPQGNKP 241
            G+KY+GRGPIQ++HNYNYGP G A+ ++LL+NPDLVATD V+SFK+A+WFWMTPQ  KP
Sbjct: 185 PGRKYFGRGPIQISHNYNYGPCGRAIGVDLLNNPDLVATDPVISFKSALWFWMTPQSPKP 244

Query: 242 SCHDVITGRWQPSSADSAAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDML 301
           SCHDVI GRWQPS+ D AA R+PG+GVITNIINGGLECG G D RV DRIGFY+RY  +L
Sbjct: 245 SCHDVIIGRWQPSAGDRAANRLPGFGVITNIINGGLECGRGTDSRVQDRIGFYRRYCSIL 304

Query: 302 GVSYGSNLDCNNQRSF 314
           GVS G NLDC NQRSF
Sbjct: 305 GVSPGDNLDCGNQRSF 320

BLAST of CmaCh03G006310 vs. ExPASy Swiss-Prot
Match: P24626 (Chitinase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht3 PE=2 SV=2)

HSP 1 Score: 462.6 bits (1189), Expect = 3.5e-129
Identity = 217/318 (68.24%), Postives = 247/318 (77.67%), Query Frame = 0

Query: 1   MKLRTLIVLAFAFVLGAAAEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKNGCQSQCGG 60
           M+   L V+A A V+    EQCG QA GALCPN LCCSQ+GWCG T +YC  GCQSQC G
Sbjct: 1   MRALALAVVAMA-VVAVRGEQCGSQAGGALCPNCLCCSQYGWCGSTSDYCGAGCQSQCSG 60

Query: 61  TTPS--TPPSSGGG-GVGSIISEALYNQMLKYSKDPRCPSNGFYQYNAFIAATQSFPGFG 120
                 TPPSSGGG GV SIIS +L++QML +  D  C + GFY Y+AF+AA  ++P F 
Sbjct: 61  GCGGGPTPPSSGGGSGVASIISPSLFDQMLLHRNDQACAAKGFYTYDAFVAAANAYPDFA 120

Query: 121 TTGDAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIREINQD--VYCSPNQQWP 180
           TTGDA T KRE+AAF  QTSHETTGGW TAPDGPY+WGYCF  E N +   YC P  +WP
Sbjct: 121 TTGDADTCKREVAAFLAQTSHETTGGWPTAPDGPYSWGYCFKEENNGNAPTYCEPKPEWP 180

Query: 181 CAAGQKYYGRGPIQLTHNYNYGPAGNALNLNLLSNPDLVATDAVVSFKTAIWFWMTPQGN 240
           CAAG+KYYGRGPIQ+T+NYNYGPAG A+  +LL+NPDLVA+DA VSFKTA WFWMTPQ  
Sbjct: 181 CAAGKKYYGRGPIQITYNYNYGPAGQAIGSDLLNNPDLVASDATVSFKTAFWFWMTPQSP 240

Query: 241 KPSCHDVITGRWQPSSADSAAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSD 300
           KPSCH VITG+W PS+ D AAGRVPGYG ITNIINGG+ECGHG DD+VADRIGFYKRY D
Sbjct: 241 KPSCHAVITGQWTPSADDQAAGRVPGYGEITNIINGGVECGHGADDKVADRIGFYKRYCD 300

Query: 301 MLGVSYGSNLDCNNQRSF 314
           MLGVSYG NLDC NQR +
Sbjct: 301 MLGVSYGDNLDCYNQRPY 317

BLAST of CmaCh03G006310 vs. ExPASy TrEMBL
Match: A0A6J1ILX6 (chitinase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111477667 PE=4 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 2.8e-190
Identity = 313/313 (100.00%), Postives = 313/313 (100.00%), Query Frame = 0

Query: 1   MKLRTLIVLAFAFVLGAAAEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKNGCQSQCGG 60
           MKLRTLIVLAFAFVLGAAAEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKNGCQSQCGG
Sbjct: 1   MKLRTLIVLAFAFVLGAAAEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKNGCQSQCGG 60

Query: 61  TTPSTPPSSGGGGVGSIISEALYNQMLKYSKDPRCPSNGFYQYNAFIAATQSFPGFGTTG 120
           TTPSTPPSSGGGGVGSIISEALYNQMLKYSKDPRCPSNGFYQYNAFIAATQSFPGFGTTG
Sbjct: 61  TTPSTPPSSGGGGVGSIISEALYNQMLKYSKDPRCPSNGFYQYNAFIAATQSFPGFGTTG 120

Query: 121 DAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIREINQDVYCSPNQQWPCAAGQ 180
           DAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIREINQDVYCSPNQQWPCAAGQ
Sbjct: 121 DAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIREINQDVYCSPNQQWPCAAGQ 180

Query: 181 KYYGRGPIQLTHNYNYGPAGNALNLNLLSNPDLVATDAVVSFKTAIWFWMTPQGNKPSCH 240
           KYYGRGPIQLTHNYNYGPAGNALNLNLLSNPDLVATDAVVSFKTAIWFWMTPQGNKPSCH
Sbjct: 181 KYYGRGPIQLTHNYNYGPAGNALNLNLLSNPDLVATDAVVSFKTAIWFWMTPQGNKPSCH 240

Query: 241 DVITGRWQPSSADSAAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDMLGVS 300
           DVITGRWQPSSADSAAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDMLGVS
Sbjct: 241 DVITGRWQPSSADSAAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDMLGVS 300

Query: 301 YGSNLDCNNQRSF 314
           YGSNLDCNNQRSF
Sbjct: 301 YGSNLDCNNQRSF 313

BLAST of CmaCh03G006310 vs. ExPASy TrEMBL
Match: A0A6J1GD14 (endochitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111453081 PE=4 SV=1)

HSP 1 Score: 654.1 bits (1686), Expect = 3.0e-184
Identity = 301/313 (96.17%), Postives = 306/313 (97.76%), Query Frame = 0

Query: 1   MKLRTLIVLAFAFVLGAAAEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKNGCQSQCGG 60
           MKL TLIVLAFAFVLG AAEQCGRQANGALCPNGLCCSQFGWCGDTDEYCK+GCQSQCGG
Sbjct: 8   MKLHTLIVLAFAFVLGGAAEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKDGCQSQCGG 67

Query: 61  TTPSTPPSSGGGGVGSIISEALYNQMLKYSKDPRCPSNGFYQYNAFIAATQSFPGFGTTG 120
           +TPSTPPSSGG GVGSIISE+LYNQMLKYSKDPRCPSNGFY+YNAF+ A QSFPGFGTTG
Sbjct: 68  STPSTPPSSGGSGVGSIISESLYNQMLKYSKDPRCPSNGFYRYNAFVTAAQSFPGFGTTG 127

Query: 121 DAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIREINQDVYCSPNQQWPCAAGQ 180
           DAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIRE NQDVYCSPNQQWPCAAGQ
Sbjct: 128 DAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYCSPNQQWPCAAGQ 187

Query: 181 KYYGRGPIQLTHNYNYGPAGNALNLNLLSNPDLVATDAVVSFKTAIWFWMTPQGNKPSCH 240
           KYYGRGPIQLTHNYNYGPAGNALNLNLL NPDLVATDAVVSFKTAIWFWMTPQGNKPSCH
Sbjct: 188 KYYGRGPIQLTHNYNYGPAGNALNLNLLGNPDLVATDAVVSFKTAIWFWMTPQGNKPSCH 247

Query: 241 DVITGRWQPSSADSAAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDMLGVS 300
           DVITGRWQPSSADSAAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDMLGVS
Sbjct: 248 DVITGRWQPSSADSAAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDMLGVS 307

Query: 301 YGSNLDCNNQRSF 314
           YGSNLDCNNQRSF
Sbjct: 308 YGSNLDCNNQRSF 320

BLAST of CmaCh03G006310 vs. ExPASy TrEMBL
Match: A0A6J1GD34 (endochitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111453082 PE=4 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 2.8e-174
Identity = 287/315 (91.11%), Postives = 297/315 (94.29%), Query Frame = 0

Query: 1   MKLRTLIVLAFAFVLGAAAEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKNGCQSQCGG 60
           MKL  LIVLAFAFVLGAAAEQCGRQANGALCPN LCCSQFGWCG+TDEYCKN CQSQCGG
Sbjct: 1   MKLHALIVLAFAFVLGAAAEQCGRQANGALCPNRLCCSQFGWCGNTDEYCKNNCQSQCGG 60

Query: 61  TTPSTPPSSGGGG--VGSIISEALYNQMLKYSKDPRCPSNGFYQYNAFIAATQSFPGFGT 120
           +TPS PPS+GGGG  VGSII+EALYNQMLKYSKDPRCPSNGFY+YNAFI A QSFP FGT
Sbjct: 61  STPSPPPSTGGGGGSVGSIINEALYNQMLKYSKDPRCPSNGFYRYNAFITAAQSFPAFGT 120

Query: 121 TGDAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIREINQDVYCSPNQQWPCAA 180
           TGDAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIRE NQDVYCSPNQQWPCAA
Sbjct: 121 TGDAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYCSPNQQWPCAA 180

Query: 181 GQKYYGRGPIQLTHNYNYGPAGNALNLNLLSNPDLVATDAVVSFKTAIWFWMTPQGNKPS 240
           GQKYYGRGPIQLTHNYNYGPAG ALNLNLL NPDLVATDAVV+FKTAIWFWMTPQGNKPS
Sbjct: 181 GQKYYGRGPIQLTHNYNYGPAGRALNLNLLGNPDLVATDAVVAFKTAIWFWMTPQGNKPS 240

Query: 241 CHDVITGRWQPSSADSAAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDMLG 300
           CHDVITGRWQPS+ADSAAGRVPGYGVITNIINGGLECG G D RVADRIGFYKRY D+LG
Sbjct: 241 CHDVITGRWQPSAADSAAGRVPGYGVITNIINGGLECGRGADSRVADRIGFYKRYCDLLG 300

Query: 301 VSYGSNLDCNNQRSF 314
           + YG+NLDCNNQRSF
Sbjct: 301 IGYGNNLDCNNQRSF 315

BLAST of CmaCh03G006310 vs. ExPASy TrEMBL
Match: O80404 (Chitinase OS=Cucurbita hybrid cultivar OX=170850 GN=chitP1 PE=2 SV=1)

HSP 1 Score: 607.4 bits (1565), Expect = 3.3e-170
Identity = 282/313 (90.10%), Postives = 291/313 (92.97%), Query Frame = 0

Query: 1   MKLRTLIVLAFAFVLGAAAEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKNGCQSQCGG 60
           MKL TLIVLAFAFVLGAAAEQCGRQANGALCPN LCCSQFGWCG+TDEYCKN CQSQC  
Sbjct: 1   MKLHTLIVLAFAFVLGAAAEQCGRQANGALCPNRLCCSQFGWCGNTDEYCKNNCQSQC-- 60

Query: 61  TTPSTPPSSGGGGVGSIISEALYNQMLKYSKDPRCPSNGFYQYNAFIAATQSFPGFGTTG 120
           T PST    GGG VGSII+EALYNQMLKYSKDPRCPSNGFY+YNAFI A QSF GFGTTG
Sbjct: 61  TPPSTGGGGGGGSVGSIINEALYNQMLKYSKDPRCPSNGFYRYNAFITAAQSFSGFGTTG 120

Query: 121 DAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIREINQDVYCSPNQQWPCAAGQ 180
           DAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIRE NQDVYCSPNQQWPCAAGQ
Sbjct: 121 DAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYCSPNQQWPCAAGQ 180

Query: 181 KYYGRGPIQLTHNYNYGPAGNALNLNLLSNPDLVATDAVVSFKTAIWFWMTPQGNKPSCH 240
           KYYGRGPIQLTHNYNYGPAG ALNLNLL+NPDLVATD VV+FKTAIWFWMTPQGNKPSCH
Sbjct: 181 KYYGRGPIQLTHNYNYGPAGRALNLNLLNNPDLVATDPVVAFKTAIWFWMTPQGNKPSCH 240

Query: 241 DVITGRWQPSSADSAAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDMLGVS 300
           DVITGRWQPS+ADSAAGRVPGYGVITNIINGGLECG G D RVADRIGFYKRY D+LG+ 
Sbjct: 241 DVITGRWQPSAADSAAGRVPGYGVITNIINGGLECGRGADSRVADRIGFYKRYCDLLGIG 300

Query: 301 YGSNLDCNNQRSF 314
           YG+NLDCNNQRSF
Sbjct: 301 YGNNLDCNNQRSF 311

BLAST of CmaCh03G006310 vs. ExPASy TrEMBL
Match: A0A6J1IJI8 (endochitinase-like OS=Cucurbita maxima OX=3661 GN=LOC111477669 PE=4 SV=1)

HSP 1 Score: 605.1 bits (1559), Expect = 1.6e-169
Identity = 283/314 (90.13%), Postives = 292/314 (92.99%), Query Frame = 0

Query: 1   MKLRTLIVLAFAFVLGAAAEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKNGCQSQCGG 60
           MKL TLIVLAFAFVLGAAAEQCGRQANGALCPN LCCSQFGWCG+TDEYCKN CQSQC  
Sbjct: 1   MKLHTLIVLAFAFVLGAAAEQCGRQANGALCPNRLCCSQFGWCGNTDEYCKNNCQSQC-- 60

Query: 61  TTPSTPPSSGGGG-VGSIISEALYNQMLKYSKDPRCPSNGFYQYNAFIAATQSFPGFGTT 120
           T PST    GGGG VGSII+EALYNQMLKYSKDPRCPSNGFY+YNAFI A QSF GFGTT
Sbjct: 61  TPPSTGGGGGGGGSVGSIINEALYNQMLKYSKDPRCPSNGFYRYNAFITAAQSFSGFGTT 120

Query: 121 GDAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIREINQDVYCSPNQQWPCAAG 180
           GDAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIRE NQDVYCSPNQQWPCAAG
Sbjct: 121 GDAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYCSPNQQWPCAAG 180

Query: 181 QKYYGRGPIQLTHNYNYGPAGNALNLNLLSNPDLVATDAVVSFKTAIWFWMTPQGNKPSC 240
           QKYYGRGPIQLTHNYNYGPAG ALNLNLL+NPDLVATD VV+FKTAIWFWMTPQGNKPSC
Sbjct: 181 QKYYGRGPIQLTHNYNYGPAGRALNLNLLNNPDLVATDPVVAFKTAIWFWMTPQGNKPSC 240

Query: 241 HDVITGRWQPSSADSAAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDMLGV 300
           HDVITGRWQPS+ADSAAGRVPGYGVITNIINGGLECG G D RVADRIGFYKRY D+LG+
Sbjct: 241 HDVITGRWQPSAADSAAGRVPGYGVITNIINGGLECGRGADSRVADRIGFYKRYCDLLGI 300

Query: 301 SYGSNLDCNNQRSF 314
            YG+NLDCNNQRSF
Sbjct: 301 GYGNNLDCNNQRSF 312

BLAST of CmaCh03G006310 vs. NCBI nr
Match: XP_022977305.1 (chitinase 1-like [Cucurbita maxima])

HSP 1 Score: 674.1 bits (1738), Expect = 5.9e-190
Identity = 313/313 (100.00%), Postives = 313/313 (100.00%), Query Frame = 0

Query: 1   MKLRTLIVLAFAFVLGAAAEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKNGCQSQCGG 60
           MKLRTLIVLAFAFVLGAAAEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKNGCQSQCGG
Sbjct: 1   MKLRTLIVLAFAFVLGAAAEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKNGCQSQCGG 60

Query: 61  TTPSTPPSSGGGGVGSIISEALYNQMLKYSKDPRCPSNGFYQYNAFIAATQSFPGFGTTG 120
           TTPSTPPSSGGGGVGSIISEALYNQMLKYSKDPRCPSNGFYQYNAFIAATQSFPGFGTTG
Sbjct: 61  TTPSTPPSSGGGGVGSIISEALYNQMLKYSKDPRCPSNGFYQYNAFIAATQSFPGFGTTG 120

Query: 121 DAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIREINQDVYCSPNQQWPCAAGQ 180
           DAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIREINQDVYCSPNQQWPCAAGQ
Sbjct: 121 DAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIREINQDVYCSPNQQWPCAAGQ 180

Query: 181 KYYGRGPIQLTHNYNYGPAGNALNLNLLSNPDLVATDAVVSFKTAIWFWMTPQGNKPSCH 240
           KYYGRGPIQLTHNYNYGPAGNALNLNLLSNPDLVATDAVVSFKTAIWFWMTPQGNKPSCH
Sbjct: 181 KYYGRGPIQLTHNYNYGPAGNALNLNLLSNPDLVATDAVVSFKTAIWFWMTPQGNKPSCH 240

Query: 241 DVITGRWQPSSADSAAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDMLGVS 300
           DVITGRWQPSSADSAAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDMLGVS
Sbjct: 241 DVITGRWQPSSADSAAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDMLGVS 300

Query: 301 YGSNLDCNNQRSF 314
           YGSNLDCNNQRSF
Sbjct: 301 YGSNLDCNNQRSF 313

BLAST of CmaCh03G006310 vs. NCBI nr
Match: KAG6603867.1 (hypothetical protein SDJN03_04476, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 654.4 bits (1687), Expect = 4.8e-184
Identity = 302/313 (96.49%), Postives = 307/313 (98.08%), Query Frame = 0

Query: 1   MKLRTLIVLAFAFVLGAAAEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKNGCQSQCGG 60
           MKL TLIVLAFAFVLG AAEQCGRQANGALCPNGLCCSQFGWCGDTDEYCK+GCQSQCGG
Sbjct: 1   MKLHTLIVLAFAFVLGGAAEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKDGCQSQCGG 60

Query: 61  TTPSTPPSSGGGGVGSIISEALYNQMLKYSKDPRCPSNGFYQYNAFIAATQSFPGFGTTG 120
           +TPSTPPSSGG GVGSIISE+LYNQMLKYSKDPRCPSNGFY+YNAF+ A QSFPGFGTTG
Sbjct: 61  STPSTPPSSGGSGVGSIISESLYNQMLKYSKDPRCPSNGFYRYNAFVTAAQSFPGFGTTG 120

Query: 121 DAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIREINQDVYCSPNQQWPCAAGQ 180
           DAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIREINQDVYCSPNQQWPCAAGQ
Sbjct: 121 DAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIREINQDVYCSPNQQWPCAAGQ 180

Query: 181 KYYGRGPIQLTHNYNYGPAGNALNLNLLSNPDLVATDAVVSFKTAIWFWMTPQGNKPSCH 240
           KYYGRGPIQLTHNYNYG AGNALNLNLLSNPDLVATDAVVSFKTAIWFWMTPQGNKPSCH
Sbjct: 181 KYYGRGPIQLTHNYNYGLAGNALNLNLLSNPDLVATDAVVSFKTAIWFWMTPQGNKPSCH 240

Query: 241 DVITGRWQPSSADSAAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDMLGVS 300
           DVITGRWQPSSADSAAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDMLGVS
Sbjct: 241 DVITGRWQPSSADSAAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDMLGVS 300

Query: 301 YGSNLDCNNQRSF 314
           YGSNLDCNNQRSF
Sbjct: 301 YGSNLDCNNQRSF 313

BLAST of CmaCh03G006310 vs. NCBI nr
Match: XP_022949791.1 (endochitinase-like [Cucurbita moschata])

HSP 1 Score: 654.1 bits (1686), Expect = 6.3e-184
Identity = 301/313 (96.17%), Postives = 306/313 (97.76%), Query Frame = 0

Query: 1   MKLRTLIVLAFAFVLGAAAEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKNGCQSQCGG 60
           MKL TLIVLAFAFVLG AAEQCGRQANGALCPNGLCCSQFGWCGDTDEYCK+GCQSQCGG
Sbjct: 8   MKLHTLIVLAFAFVLGGAAEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKDGCQSQCGG 67

Query: 61  TTPSTPPSSGGGGVGSIISEALYNQMLKYSKDPRCPSNGFYQYNAFIAATQSFPGFGTTG 120
           +TPSTPPSSGG GVGSIISE+LYNQMLKYSKDPRCPSNGFY+YNAF+ A QSFPGFGTTG
Sbjct: 68  STPSTPPSSGGSGVGSIISESLYNQMLKYSKDPRCPSNGFYRYNAFVTAAQSFPGFGTTG 127

Query: 121 DAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIREINQDVYCSPNQQWPCAAGQ 180
           DAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIRE NQDVYCSPNQQWPCAAGQ
Sbjct: 128 DAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYCSPNQQWPCAAGQ 187

Query: 181 KYYGRGPIQLTHNYNYGPAGNALNLNLLSNPDLVATDAVVSFKTAIWFWMTPQGNKPSCH 240
           KYYGRGPIQLTHNYNYGPAGNALNLNLL NPDLVATDAVVSFKTAIWFWMTPQGNKPSCH
Sbjct: 188 KYYGRGPIQLTHNYNYGPAGNALNLNLLGNPDLVATDAVVSFKTAIWFWMTPQGNKPSCH 247

Query: 241 DVITGRWQPSSADSAAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDMLGVS 300
           DVITGRWQPSSADSAAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDMLGVS
Sbjct: 248 DVITGRWQPSSADSAAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDMLGVS 307

Query: 301 YGSNLDCNNQRSF 314
           YGSNLDCNNQRSF
Sbjct: 308 YGSNLDCNNQRSF 320

BLAST of CmaCh03G006310 vs. NCBI nr
Match: XP_023544980.1 (endochitinase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 651.0 bits (1678), Expect = 5.3e-183
Identity = 300/313 (95.85%), Postives = 305/313 (97.44%), Query Frame = 0

Query: 1   MKLRTLIVLAFAFVLGAAAEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKNGCQSQCGG 60
           MKL TLIVLAFAFVLG AAEQCGRQANGALCPNGLCCSQFGWCGDTDEYCK+GCQSQCGG
Sbjct: 1   MKLHTLIVLAFAFVLGGAAEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKDGCQSQCGG 60

Query: 61  TTPSTPPSSGGGGVGSIISEALYNQMLKYSKDPRCPSNGFYQYNAFIAATQSFPGFGTTG 120
           +TPSTPPSSGG GVGSIISEALYNQMLKYSKDPRCPSNGFY+YNAF+ A QSFPGFGTTG
Sbjct: 61  STPSTPPSSGGSGVGSIISEALYNQMLKYSKDPRCPSNGFYRYNAFVTAAQSFPGFGTTG 120

Query: 121 DAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIREINQDVYCSPNQQWPCAAGQ 180
           D ATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIRE NQDVYCSPNQQWPCAAGQ
Sbjct: 121 DTATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYCSPNQQWPCAAGQ 180

Query: 181 KYYGRGPIQLTHNYNYGPAGNALNLNLLSNPDLVATDAVVSFKTAIWFWMTPQGNKPSCH 240
           KYYGRGPIQLTHNYNYGPAGNALNLNLLSNPDLVATDAVV+FKTAIWFWMTPQGNKPSCH
Sbjct: 181 KYYGRGPIQLTHNYNYGPAGNALNLNLLSNPDLVATDAVVAFKTAIWFWMTPQGNKPSCH 240

Query: 241 DVITGRWQPSSADSAAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDMLGVS 300
           DVITGRWQPSSADSAAGRV GYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDMLGVS
Sbjct: 241 DVITGRWQPSSADSAAGRVAGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDMLGVS 300

Query: 301 YGSNLDCNNQRSF 314
           YGSNLDCNNQRSF
Sbjct: 301 YGSNLDCNNQRSF 313

BLAST of CmaCh03G006310 vs. NCBI nr
Match: XP_023544979.1 (endochitinase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 625.2 bits (1611), Expect = 3.1e-175
Identity = 287/313 (91.69%), Postives = 296/313 (94.57%), Query Frame = 0

Query: 1   MKLRTLIVLAFAFVLGAAAEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKNGCQSQCGG 60
           MKL TLIVLAFAFVLG AAEQCGRQANGALCPN LCCSQFGWCG+TDEYCKN CQSQCGG
Sbjct: 1   MKLHTLIVLAFAFVLGGAAEQCGRQANGALCPNRLCCSQFGWCGNTDEYCKNNCQSQCGG 60

Query: 61  TTPSTPPSSGGGGVGSIISEALYNQMLKYSKDPRCPSNGFYQYNAFIAATQSFPGFGTTG 120
           +TPSTPPS+GG GVG IISEALYNQMLKYSKDPRCPSNGFY+YNAFI A QSF GFGTTG
Sbjct: 61  STPSTPPSTGGSGVGGIISEALYNQMLKYSKDPRCPSNGFYRYNAFITAAQSFSGFGTTG 120

Query: 121 DAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIREINQDVYCSPNQQWPCAAGQ 180
           DAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIRE NQDVYCSPNQQWPCAAGQ
Sbjct: 121 DAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYCSPNQQWPCAAGQ 180

Query: 181 KYYGRGPIQLTHNYNYGPAGNALNLNLLSNPDLVATDAVVSFKTAIWFWMTPQGNKPSCH 240
           KYYGRGPIQLTHNYNYGPAG ALNLNLL NPDLVATDAVV+FKTAIWFWMTPQGNKPSCH
Sbjct: 181 KYYGRGPIQLTHNYNYGPAGRALNLNLLGNPDLVATDAVVAFKTAIWFWMTPQGNKPSCH 240

Query: 241 DVITGRWQPSSADSAAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDMLGVS 300
           DVITGRWQPS+ADSAAGRVPGYGVITNIINGGLECG G D RVADRIGFYKRY D+LG+ 
Sbjct: 241 DVITGRWQPSAADSAAGRVPGYGVITNIINGGLECGRGADSRVADRIGFYKRYCDLLGIG 300

Query: 301 YGSNLDCNNQRSF 314
           YG+NLDCNNQRSF
Sbjct: 301 YGNNLDCNNQRSF 313

BLAST of CmaCh03G006310 vs. TAIR 10
Match: AT3G12500.1 (basic chitinase )

HSP 1 Score: 453.4 bits (1165), Expect = 1.5e-127
Identity = 210/316 (66.46%), Postives = 247/316 (78.16%), Query Frame = 0

Query: 1   MKLRTLIVLAFAFVLG-AAAEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKN-GCQSQC 60
           MK    + L F+ +L  ++AEQCGRQA GALCPNGLCCS+FGWCG+T+ YCK  GCQSQC
Sbjct: 14  MKTNLFLFLIFSLLLSLSSAEQCGRQAGGALCPNGLCCSEFGWCGNTEPYCKQPGCQSQC 73

Query: 61  GGTTPSTPPSSGGGGVGSIISEALYNQMLKYSKDPRCPSNGFYQYNAFIAATQSFPGFGT 120
              TP   P    G +  IIS + ++ MLK+  D  CP+ GFY YNAFI A +SFPGFGT
Sbjct: 74  ---TPGGTPPGPTGDLSGIISSSQFDDMLKHRNDAACPARGFYTYNAFITAAKSFPGFGT 133

Query: 121 TGDAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIREIN-QDVYCSPNQQWPCA 180
           TGD ATRK+E+AAFFGQTSHETTGGWATAPDGPY+WGYCF +E N    YC P+  WPCA
Sbjct: 134 TGDTATRKKEVAAFFGQTSHETTGGWATAPDGPYSWGYCFKQEQNPASDYCEPSATWPCA 193

Query: 181 AGQKYYGRGPIQLTHNYNYGPAGNALNLNLLSNPDLVATDAVVSFKTAIWFWMTPQGNKP 240
           +G++YYGRGP+QL+ NYNYG  G A+ ++LL+NPDLVA DAV++FK AIWFWMT Q  KP
Sbjct: 194 SGKRYYGRGPMQLSWNYNYGLCGRAIGVDLLNNPDLVANDAVIAFKAAIWFWMTAQPPKP 253

Query: 241 SCHDVITGRWQPSSADSAAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDML 300
           SCH VI G+WQPS AD AAGR+PGYGVITNIINGGLECG G D RVADRIGFY+RY ++ 
Sbjct: 254 SCHAVIAGQWQPSDADRAAGRLPGYGVITNIINGGLECGRGQDGRVADRIGFYQRYCNIF 313

Query: 301 GVSYGSNLDCNNQRSF 314
           GV+ G NLDC NQRSF
Sbjct: 314 GVNPGGNLDCYNQRSF 326

BLAST of CmaCh03G006310 vs. TAIR 10
Match: AT4G01700.1 (Chitinase family protein )

HSP 1 Score: 308.1 bits (788), Expect = 7.9e-84
Identity = 137/240 (57.08%), Postives = 178/240 (74.17%), Query Frame = 0

Query: 76  SIISEALYNQMLKYSKDPRCPSNGFYQYNAFIAATQSFPGFGTTGDAATRKRELAAFFGQ 135
           S++   LY+Q+  +  +  CP+ GFY Y AF+ AT+SFP FG+ G+  TR+RE+AAF  Q
Sbjct: 40  SLVPRTLYDQIFIHKDNNACPAKGFYPYEAFVEATRSFPKFGSVGNFWTRRREVAAFLAQ 99

Query: 136 TSHETTGGWATAPDGPYAWGYCFIREIN-QDVYC-SPNQQWPCAAGQKYYGRGPIQLTHN 195
            SHETTGGWATAPDGPYAWG CF  E++ Q  YC + N+ WPC +G+ Y GRGPIQL+ N
Sbjct: 100 ISHETTGGWATAPDGPYAWGLCFKEEVSPQSNYCDASNKDWPCVSGKSYKGRGPIQLSWN 159

Query: 196 YNYGPAGNALNLNLLSNPDLVATDAVVSFKTAIWFWMTPQGNKPSCHDVITGRWQPSSAD 255
           YNYG AG AL  + L NP+LVA ++V++FKTA+WFWMT Q  KPSCH+V+  R++P+ AD
Sbjct: 160 YNYGQAGRALGFDGLQNPELVANNSVLAFKTALWFWMTEQTPKPSCHNVMVNRYRPTKAD 219

Query: 256 SAAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDMLGVSYGSNLDCNNQRSF 314
            AA R  GYG++TNIINGGLECG   D RV DR+G+++RY+ +  V+ G NLDC NQR F
Sbjct: 220 RAANRTVGYGLVTNIINGGLECGIPGDGRVTDRVGYFQRYAQLFKVTTGPNLDCENQRPF 279

BLAST of CmaCh03G006310 vs. TAIR 10
Match: AT1G02360.1 (Chitinase family protein )

HSP 1 Score: 302.0 bits (772), Expect = 5.6e-82
Identity = 133/239 (55.65%), Postives = 175/239 (73.22%), Query Frame = 0

Query: 77  IISEALYNQMLKYSKDPRCPSNGFYQYNAFIAATQSFPGFGTTGDAATRKRELAAFFGQT 136
           ++   LYN++  +  +  CP+NGFY Y +F+ AT+ FP FG+ G   T++ E+AAF  Q 
Sbjct: 33  LVPRDLYNKIFIHKDNTACPANGFYTYESFVQATRRFPRFGSVGSPVTQRLEVAAFLAQI 92

Query: 137 SHETTGGWATAPDGPYAWGYCFIREIN-QDVYC-SPNQQWPCAAGQKYYGRGPIQLTHNY 196
           SHETTGGWATAPDGPYAWG CF  E++ Q  YC S + QWPC   + Y GRGPIQL+ NY
Sbjct: 93  SHETTGGWATAPDGPYAWGLCFKEEVSPQSTYCDSSDTQWPCFPNKTYQGRGPIQLSWNY 152

Query: 197 NYGPAGNALNLNLLSNPDLVATDAVVSFKTAIWFWMTPQGNKPSCHDVITGRWQPSSADS 256
           NYGPAG AL  + L NP+ V+ ++V++F+TA+WFWMTPQ  KPSCHDV+ G+++P++AD 
Sbjct: 153 NYGPAGRALGFDGLRNPETVSNNSVIAFQTALWFWMTPQSPKPSCHDVMIGKYRPTAADL 212

Query: 257 AAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDMLGVSYGSNLDCNNQRSF 314
           AA R  G+G+ TNIINGGLECG   D RV DRIGF++RY+ +  V+ G NLDC NQR +
Sbjct: 213 AANRTGGFGLTTNIINGGLECGIPGDGRVNDRIGFFQRYTGLFKVATGPNLDCENQRPY 271

BLAST of CmaCh03G006310 vs. TAIR 10
Match: AT3G54420.1 (homolog of carrot EP3-3 chitinase )

HSP 1 Score: 191.0 bits (484), Expect = 1.4e-48
Identity = 114/311 (36.66%), Postives = 156/311 (50.16%), Query Frame = 0

Query: 1   MKLRTLIVLAFAFVLGAAAEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKNGC-QSQCG 60
           + L T++++  AF     A+ CG       C + LCCSQFG+CG+T +YC  GC Q  C 
Sbjct: 10  ISLVTILLVLQAFSNTTKAQNCG-------CSSELCCSQFGFCGNTSDYCGVGCQQGPC- 69

Query: 61  GTTPSTPPSSGGGGVGSIISEALYNQMLKYSKDPRCPSNGFYQYNAFIAATQSFPGFGTT 120
                 PP + G  V  I+++  +N ++  +    C  N FY   AF+ A  S+  FG  
Sbjct: 70  ----FAPPPANGVSVAEIVTQEFFNGIISQAAS-SCAGNRFYSRGAFLEALDSYSRFGRV 129

Query: 121 GDAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIREIN--QDVYCSPN-QQWPC 180
           G     +RE+AAFF   +HET               +C+I EI+     YC  N  Q+PC
Sbjct: 130 GSTDDSRREIAAFFAHVTHET-------------GHFCYIEEIDGASKDYCDENATQYPC 189

Query: 181 AAGQKYYGRGPIQLTHNYNYGPAGNALNLNLLSNPDLVATDAVVSFKTAIWFWMTPQGNK 240
              + YYGRGPIQL+ N+NYGPAG A+  + L+ P+ VATD V+SFKTA+W+W       
Sbjct: 190 NPNKGYYGRGPIQLSWNFNYGPAGTAIGFDGLNAPETVATDPVISFKTALWYW------- 249

Query: 241 PSCHDVITGRWQPSSADSAAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDM 300
                  T R QP  +        G+G     ING LEC       V  R+ +Y  Y   
Sbjct: 250 -------TNRVQPVISQ-------GFGATIRAINGALECDGANTATVQARVRYYTDYCRQ 273

Query: 301 LGVSYGSNLDC 308
           LGV  G+NL C
Sbjct: 310 LGVDPGNNLTC 273

BLAST of CmaCh03G006310 vs. TAIR 10
Match: AT2G43610.1 (Chitinase family protein )

HSP 1 Score: 189.5 bits (480), Expect = 4.1e-48
Identity = 118/316 (37.34%), Postives = 158/316 (50.00%), Query Frame = 0

Query: 4   RTLIVLAFAFVL---GAAAEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKNGCQS-QCG 63
           + LI+  F   +    A ++ CG   NG  C   +CCS++G+CG T  YC  GCQS  C 
Sbjct: 10  KALIIFLFTLTIMTGTAFSQNCG--TNG--CKGNMCCSRWGYCGTTKAYCGTGCQSGPCN 69

Query: 64  GTTPSTPPSSGGGG--------VGSIISEALYNQMLKYSKDPRCPSNGFYQYNAFIAATQ 123
                TP  SG GG        + S+I+ A +N ++       CP+ GFY   AFIAA +
Sbjct: 70  SKPKPTPTPSGSGGLNAGPRGTIASVITPAFFNSIMS-KVGSGCPAKGFYTRQAFIAAAE 129

Query: 124 SFPGFGTTGDAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIREINQDVYCSPN 183
           SF  +  T      KRE+AA   Q SHE             +  +C+  EI +  YCSP+
Sbjct: 130 SFAAYKGT----VAKREIAAMLAQFSHE-------------SGSFCYKEEIARGRYCSPS 189

Query: 184 QQWPCAAGQKYYGRGPIQLTHNYNYGPAGNALNLNLLSNPDLVATDAVVSFKTAIWFWMT 243
             +PC  G+ YYGRGPIQ+T NYNYG AG  L L LL +PD+VA    V+F+ A+WFW  
Sbjct: 190 TTYPCQPGKNYYGRGPIQITWNYNYGAAGKFLGLPLLKDPDMVARSPTVAFQCAMWFW-- 249

Query: 244 PQGNKPSCHDVITGRWQPSSADSAAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYK 303
              NK +   V++                G+G  T  INGG EC  G    V  R+  Y 
Sbjct: 250 ---NK-NVRPVLS---------------QGFGATTRRINGG-ECNGGRPAAVQSRVNHYL 281

Query: 304 RYSDMLGVSYGSNLDC 308
            +   LGV+ G+NL C
Sbjct: 310 DFCKKLGVTPGTNLSC 281

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P936801.1e-13370.29Endochitinase OS=Persea americana OX=3435 GN=chi1 PE=1 SV=2[more]
Q429931.5e-13268.54Chitinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht1 PE=2 SV=1[more]
P369074.4e-13268.99Endochitinase OS=Pisum sativum OX=3888 PE=2 SV=1[more]
P082522.4e-13067.72Endochitinase A OS=Nicotiana tabacum OX=4097 GN=CHN48 PE=1 SV=2[more]
P246263.5e-12968.24Chitinase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht3 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1ILX62.8e-190100.00chitinase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111477667 PE=4 SV=1[more]
A0A6J1GD143.0e-18496.17endochitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111453081 PE=4 SV=1[more]
A0A6J1GD342.8e-17491.11endochitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111453082 PE=4 SV=1[more]
O804043.3e-17090.10Chitinase OS=Cucurbita hybrid cultivar OX=170850 GN=chitP1 PE=2 SV=1[more]
A0A6J1IJI81.6e-16990.13endochitinase-like OS=Cucurbita maxima OX=3661 GN=LOC111477669 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_022977305.15.9e-190100.00chitinase 1-like [Cucurbita maxima][more]
KAG6603867.14.8e-18496.49hypothetical protein SDJN03_04476, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022949791.16.3e-18496.17endochitinase-like [Cucurbita moschata][more]
XP_023544980.15.3e-18395.85endochitinase-like [Cucurbita pepo subsp. pepo][more]
XP_023544979.13.1e-17591.69endochitinase-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G12500.11.5e-12766.46basic chitinase [more]
AT4G01700.17.9e-8457.08Chitinase family protein [more]
AT1G02360.15.6e-8255.65Chitinase family protein [more]
AT3G54420.11.4e-4836.66homolog of carrot EP3-3 chitinase [more]
AT2G43610.14.1e-4837.34Chitinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001002Chitin-binding, type 1PRINTSPR00451CHITINBINDNGcoord: 29..37
score: 69.78
coord: 44..51
score: 53.0
coord: 37..44
score: 80.67
IPR001002Chitin-binding, type 1SMARTSM00270ChitinBD_3coord: 21..58
e-value: 4.2E-16
score: 69.5
IPR001002Chitin-binding, type 1PFAMPF00187Chitin_bind_1coord: 21..58
e-value: 1.4E-14
score: 54.2
IPR001002Chitin-binding, type 1PROSITEPS50941CHIT_BIND_I_2coord: 19..60
score: 11.000197
IPR016283Glycoside hydrolase, family 19PIRSFPIRSF001060Endochitinasecoord: 1..313
e-value: 7.8E-125
score: 414.2
IPR000726Glycoside hydrolase, family 19, catalyticPFAMPF00182Glyco_hydro_19coord: 77..307
e-value: 3.0E-127
score: 423.1
IPR000726Glycoside hydrolase, family 19, catalyticPROSITEPS00774CHITINASE_19_2coord: 220..230
IPR036861Endochitinase-like superfamilyGENE3D3.30.60.10coord: 19..61
e-value: 5.2E-18
score: 66.9
IPR036861Endochitinase-like superfamilySUPERFAMILY57016Plant lectins/antimicrobial peptidescoord: 21..65
NoneNo IPR availableGENE3D1.10.530.10coord: 77..308
e-value: 4.5E-119
score: 398.2
NoneNo IPR availableGENE3D3.30.20.10Endochitinase, domain 2coord: 160..216
e-value: 4.5E-119
score: 398.2
NoneNo IPR availablePIRSRPIRSR001060-2PIRSR001060-2coord: 3..313
e-value: 3.4E-142
score: 471.5
NoneNo IPR availablePANTHERPTHR22595CHITINASE-RELATEDcoord: 6..313
NoneNo IPR availablePANTHERPTHR22595:SF171CHITINASE (CLASS IB) / HEVEINcoord: 6..313
NoneNo IPR availableCDDcd00325chitinase_GH19coord: 80..307
e-value: 6.47155E-106
score: 306.279
NoneNo IPR availableCDDcd06921ChtBD1_GH19_heveincoord: 20..59
e-value: 4.78636E-19
score: 77.1444
IPR018371Chitin-binding, type 1, conserved sitePROSITEPS00026CHIT_BIND_I_1coord: 31..50
IPR023346Lysozyme-like domain superfamilySUPERFAMILY53955Lysozyme-likecoord: 74..313

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh03G006310.1CmaCh03G006310.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0016998 cell wall macromolecule catabolic process
biological_process GO:0006032 chitin catabolic process
biological_process GO:0006952 defense response
molecular_function GO:0004568 chitinase activity
molecular_function GO:0008061 chitin binding