Cla97C11G209100 (gene) Watermelon (97103) v2.5

Overview
NameCla97C11G209100
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
DescriptiontRNA (guanine(37)-N1)-methyltransferase
LocationCla97Chr11: 2675475 .. 2680352 (+)
RNA-Seq ExpressionCla97C11G209100
SyntenyCla97C11G209100
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCGGTCTTCACGCAGTCTCCGGCGACAGTTCGTGTTGTTAGTTTTATTCCCAGTGGAAAATCTCCGATTCCAGTCCTCTATTTCCGATCTGTTATTGCTTGGAGGACGATAATTAATCCATTCTGATTTGAATTTGTTCGCATTGAGTGCGGAGTATTGAGGAAAATCGAACTTGTGAATCGATCAATGTTGGACGAAAGCAAATTCGATGTGCATCTGAATCTCTGGGCCCTAAGAATTCCGAGGGAACTCTGTAAGGCCGCCATTAAAATACTGAACGGGTGCGTGATGTTTTGTGTTCTTTTGTTTCTTTATTATACGTTGCTAATTATTCGATTTCAATGAATCATTTGGGTATTGGCATTTAGTATTAGTGTTACTCATACATTGTACTTGTTCAACTGTTCATCTTTCTCATCTGAGTTCAACATTTTCTATTTTTATTGAAAACTTATCAGAATTTTCCTGATAGTTTTTAGTATTTTCTTTGAAAATTCATCTTGATATTGACTTTTGTTTTAAAGTAGACTGTTCTTTGGTCCTCAGTTCGTTTCTGTTGAAAAATTTTCAGGTACTTAATTGACAGGCCACGGATTAAACCTGTGACTGAAGATCCAACTTGTGATAAGAATCGTTATGTTATATTATCTGAAAAGGTTCAAACTCCGGGTAAAGATCTCCGTCCTTGCTTTTTTTCACTTGGTTTTTTCTGTTGACTATATGTTTGGATGGCAATTCCATGTTCTTTATGGAAACTCTTCAATAGAACATTCTGCCCGCTGAAGTTCCTATCCTTGTTCATTGATTCTTTTATGTTACCTTAGTGTCTTGTGGGCTCTGTTCACGCACTCAAGTTCTAAAATAGTTGTTATAAAAAATTGAGCGAGTCATTGTTAATCTTAAAATTTTAATTTCTCTAGGTATAACCATGAACTTGAATGCATGTTAAGTTAGAAAATTGTCCAAAAACAGCATATATATCTCATGAAATCCAGCTAAAAAAGAATCTAAGCATCCACAGAAGCAACCCGATTAACCTTGAGTAACTGGGTAATCATCATAAAAATAGTCTTGTTTTCTTTTGGAAATCTTAATCACGTTTCTTCATTCTTCTTTTGCCCTTATTTTCTGGATGGAATTTTTCTCAATCATGTCTTTAGATGACACCATTTTTCTATCTTGTGATTCTTTGGATGGGTCAGAATTATCAGAAATTCCAGAAATGAAGCTTGAAGAATTGAAAGGTTTATGCACGATTGAACGAGTTCCTTATTCTCTAACCCTTGGTTATTCATATTGGGGAGCAGGTATTTTCAATAATCTTACCTTTTATCACAAATTGTGCAAATTTTACTTTCACCTTAGTATTGCTTGTTGAATTCTAGACATTTATGCTTGCCTTTAATTTTCTTATTACAAGTGCGAAATGTTATTGAAGGCATATCTAGGACCATTTTTAATGATCCCATTACTCAAGTATGCACCGAGTATTGAAGTATGTGGAAGCTAAATGTTGTACTGTGTTTGCATGAGAAGGATGAGAGTCTAATACATTAGTTATTGATCATAAATGTACAGTCCCTGCATGTTTGTGCTTGAATTCTATAGTACATTTTCCATTTTATAATTTGTCTATATTAATGGGATTTATGTTCCAAACGTTGTCTGCATTGTGACTGTACATGTGCTGATGGACTGGTTGTCCTTGACTCTCATTTGATATTATGACAACTATATGTGATGTGACCGTGTCTACTTCAAGTGATTGCAGTAATAAGAATGACGATCAATTTCTGAAAGATCGTTTAAACTATTTTAAAGGTGGTTTTACCTGTCGTTTGATATGTTCATATTGTACATGTCCGACTAATTTCTGTCTGGTTCCTCTGTAGACCATATCCTCAAGCAGATTCTGCCTCCCGAAGTGGAAGTGCCTTCTTCTTTTGAGACAATAGTTAAGTACTCTAGCCACTGCCATGTTTCACCCACTTTTTTCCTTTCTGCCTTGCATTTCCATATGCTCTATATACCTTTTATGTGCGTTCTTTTGTTGCTCTGTATAGAATTTTGGTACCACTTGTTTCTCTAGCATTGCTCTTAACCTTGTTTTCTCTTGTACAACTATTGACAGTGAGATAATTTTGTACTGTGATTTACAGGTCATGTTGCTCACCTGAATATACACGACGAATTGCTTCCATATAAGGATGTCATTGCGAAAGTCATTTATGATGTAAGTGATCGATATCTACTTACTCATTTTTCTAATTAATTTGAAACGTAAATTGCGATTTATTGATCACTATACTTTAGCTGTTCATTTTCTTTATTCTTAATTCTATCTTGCTCTCCCCTTCTTTTCCTCCGCGCGCGTGTGTGTGGGGGGGGGGGGGGGTACCTTTCCCAGGAATTCAACTGTTCAGAATCAGATATTTTAACCTCCCCCCAACAATCTGCATGCACACACACACATTTTCCTTATTGAAACTTTTTTGTAACTATTTCTTTTCGGAATCTAATTACAGAAAAATTATCCGAGAATTAAAACAGTTGTAAATAAAGTGGGGTCTATAACAAATGAGTTTCGGGTTCCAAAGTTTGAAATACTGAAGGGAGAGAATGACATGGTAACAGAAGTGAAGCAATATGGAGCAACTTTCAAGCTTGACTACAGTTTGGTCTACTGGAACTCAAGACTGGAACATGAACACATAAGGCTGGTTTCACTATTCCAACCAGGAGAGATAATTTGTGACATGTTTGCTGGTATTGGTCCGTTTGCTATTCCAGCTGCCCAGAAAGAATGTATTGTATATGCCAATGACTTGAATCCAGACAGTGTTCGATATCTGAAGGTTAATGCAGAAATCAATAAAGTTAGTGGTCGTGTTCATGTATATAATTTGGATGCTAGGAAGTTCATTTCTCAATTGATGACAGTTCCCAGTAATGAAAGTACTTCAGAATCTGTCACGTCAATGTTAAAAACTTGTGAGAAAGGCATCGGAGAGACCAATCAAGAATCACCATCTGCAAATGGTATGCAGACTAGTATTATTATCATTTAAAGAAATTTTCATATCTTCTTCAGGAGATTGAGTGTGGATGATCTTAGTTTTCTTTCTTGCTTCAGTTCAGGTAGAAGAAGTCCAAGAGAATATTAAAATTAGCGATGAATGTGGGAGTGGCGATACATCTGTTGCTGCTCTTAAAAGATCCTCAGAAAGTTGTGAGAATGAGAAAGGTAACATATCATCACAGTCACAGCCAAGCAGGAGGCTGCACATTACCATATCTTGTGATTGAACAGTCAAAGACCATTTCTTCCTGTAATTTTCTTTGCAGACATTTTTTTTAAAATGAAAATTTGCTTTGCAGACAATGGTATTGCTGATGATGCTTCCATCTGTGTAGCCGGTAGGAGAAATGGAAATGCTAACAAGAGGATGAAGGGGTCCAATATATCCCATGCTAGGACCTGGGAGCATGTTGATCATGTGGTTATGAACCTCCCTGCTTCTGCTTTACATTTTCTTGGTACGAATGGACTTTTTCTTTTAAATTTCATTACCCTTATTGCAGTTGTTTTAGCATATTTCTCAACATTTGTATGTCCGAATGAAGAGTTGAAAATTAGTGAAATAACTAGGTTTGGTCACCTCTGCCAGCATGAGATTTGAGAACGGTGGAAAGGGAAGCAATAACTTTTTAATGCTCAAATATGAAGGGCAACCATAGATTTTATTTATTTTTTTCTCGTACAAGCACCATGACAGAGATATTTTGGATGTCATATTTCCTACCTTTCATCCAAGTTTTGTAGTCACTCTCTTTTTTTGATAGATACCAAGTGTATGAGTTACTTCAATCTCCTTTAGCTCTTGGGTGAGGGGGAATATCTTATCTTCTCTACTCAATTATCTTAACTCTTTTTCTGTATCTGTAACTATATTTTGTTTAGTTTAACTTTAACCAGTGAAGTATATTTTGGTATTTTCTTGCCAGATGCATTTAGGGGCACAATTAAGAGGAAGTACTGGAAGGGCTCCCTTCCTTGGATTCATTGTTATTGCTTCATGCGAGCAAATGAAAATCAAGAGTTTATCATTTCGGTATGTTCATACTACAGCTAACATCATAGCTTCATCATCTTTCAATTGTACCTGTATTCATTTGATATGTGTGCCACATGTAAGTGCATGCTTATTTCTTTGTCTTCTTTTTGATAATTGTATTTCTTCCTCACTCTTCTATTTTGGATATTTGTCTTCGAAAATGACATCTTTCTTCTCATGCGATCCTTTTTTTAACTTTTCACTCCAGGAGGCTGAGTCTGCCTTGAATGCTAATATTCAAGACCCCATTTTTCATAGGGTCAGAGATGTTGCACCAAACAAGGTATTGCTCTGTGTGTGTGTGCGTGTACACTTGGAAATTATTTGTTTTAACTTGTATTTTTGTTTTTATCAGGCAATGTACTGTTTGAGCTTTAGGCTTCCGGAACAATGCGTCAATGAAGACACGATTGGCTGAATCAAGTAAAACCTTAACTTTCCTATCTCTTGTTCTTATTGAGAAAAACTTCATTTTTCCAATTTGTTCGTGCAGAGTTTTCATAGCTCTCACATAGTTGTAATTCACAATCGTTTCTCATTTCTGAGATGTACAAGCTAGAAGTATGATATGGTAGGCTGGTGCTTAGGTGTTTCAGTTCTAAAGTTGAGATCAGGGCTAAGGTACAAAATGGGTTTGGAAAATTTCCCAGATTAACTCGGTGCAATTCCATTTCTATTCACTTGATTTTGTATTAAACGCAATATATCTCATGTCTTAAGTTATAACAATACCCCTTAACACGTAGCATGTGCTATGATGAAG

mRNA sequence

CCGGTCTTCACGCAGTCTCCGGCGACAGTTCGTGTTGTTAGTTTTATTCCCAGTGGAAAATCTCCGATTCCAGTCCTCTATTTCCGATCTGTTATTGCTTGGAGGACGATAATTAATCCATTCTGATTTGAATTTGTTCGCATTGAGTGCGGAGTATTGAGGAAAATCGAACTTGTGAATCGATCAATGTTGGACGAAAGCAAATTCGATGTGCATCTGAATCTCTGGGCCCTAAGAATTCCGAGGGAACTCTGTAAGGCCGCCATTAAAATACTGAACGGGTACTTAATTGACAGGCCACGGATTAAACCTGTGACTGAAGATCCAACTTGTGATAAGAATCGTTATGTTATATTATCTGAAAAGGTTCAAACTCCGGATGACACCATTTTTCTATCTTGTGATTCTTTGGATGGGTCAGAATTATCAGAAATTCCAGAAATGAAGCTTGAAGAATTGAAAGGTTTATGCACGATTGAACGAGTTCCTTATTCTCTAACCCTTGGTTATTCATATTGGGGAGCAGACCATATCCTCAAGCAGATTCTGCCTCCCGAAGTGGAAGTGCCTTCTTCTTTTGAGACAATAGTTAAGTACTCTAGCCACTGCCATGTTTCACCCACTTTTTTCCTTTCTGCCTTGCATTTCCATATGCTCTATATACCTTTTATGTGCGTTCTTTTGTTGCTCTGTCATGTTGCTCACCTGAATATACACGACGAATTGCTTCCATATAAGGATGTCATTGCGAAAGTCATTTATGATAAAAATTATCCGAGAATTAAAACAGTTGTAAATAAAGTGGGGTCTATAACAAATGAGTTTCGGGTTCCAAAGTTTGAAATACTGAAGGGAGAGAATGACATGGTAACAGAAGTGAAGCAATATGGAGCAACTTTCAAGCTTGACTACAGTTTGGTCTACTGGAACTCAAGACTGGAACATGAACACATAAGGCTGGTTTCACTATTCCAACCAGGAGAGATAATTTGTGACATGTTTGCTGGTATTGGTCCGTTTGCTATTCCAGCTGCCCAGAAAGAATGTATTGTATATGCCAATGACTTGAATCCAGACAGTGTTCGATATCTGAAGGTTAATGCAGAAATCAATAAAGTTAGTGGTCGTGTTCATGTATATAATTTGGATGCTAGGAAGTTCATTTCTCAATTGATGACAGTTCCCAGTAATGAAAGTACTTCAGAATCTGTCACGTCAATGTTAAAAACTTGTGAGAAAGGCATCGGAGAGACCAATCAAGAATCACCATCTGCAAATGTTCAGGTAGAAGAAGTCCAAGAGAATATTAAAATTAGCGATGAATGTGGGAGTGGCGATACATCTGTTGCTGCTCTTAAAAGATCCTCAGAAAGTTGTGAGAATGAGAAAGACAATGGTATTGCTGATGATGCTTCCATCTGTGTAGCCGGTAGGAGAAATGGAAATGCTAACAAGAGGATGAAGGGGTCCAATATATCCCATGCTAGGACCTGGGAGCATGTTGATCATGTGGTTATGAACCTCCCTGCTTCTGCTTTACATTTTCTTGATGCATTTAGGGGCACAATTAAGAGGAAGTACTGGAAGGGCTCCCTTCCTTGGATTCATTGTTATTGCTTCATGCGAGCAAATGAAAATCAAGAGTTTATCATTTCGGAGGCTGAGTCTGCCTTGAATGCTAATATTCAAGACCCCATTTTTCATAGGGTCAGAGATGTTGCACCAAACAAGGCAATGTACTGTTTGAGCTTTAGGCTTCCGGAACAATGCGTCAATGAAGACACGATTGGCTGAATCAAGTAAAACCTTAACTTTCCTATCTCTTGTTCTTATTGAGAAAAACTTCATTTTTCCAATTTGTTCGTGCAGAGTTTTCATAGCTCTCACATAGTTGTAATTCACAATCGTTTCTCATTTCTGAGATGTACAAGCTAGAAGTATGATATGGTAGGCTGGTGCTTAGGTGTTTCAGTTCTAAAGTTGAGATCAGGGCTAAGGTACAAAATGGGTTTGGAAAATTTCCCAGATTAACTCGGTGCAATTCCATTTCTATTCACTTGATTTTGTATTAAACGCAATATATCTCATGTCTTAAGTTATAACAATACCCCTTAACACGTAGCATGTGCTATGATGAAG

Coding sequence (CDS)

ATGTTGGACGAAAGCAAATTCGATGTGCATCTGAATCTCTGGGCCCTAAGAATTCCGAGGGAACTCTGTAAGGCCGCCATTAAAATACTGAACGGGTACTTAATTGACAGGCCACGGATTAAACCTGTGACTGAAGATCCAACTTGTGATAAGAATCGTTATGTTATATTATCTGAAAAGGTTCAAACTCCGGATGACACCATTTTTCTATCTTGTGATTCTTTGGATGGGTCAGAATTATCAGAAATTCCAGAAATGAAGCTTGAAGAATTGAAAGGTTTATGCACGATTGAACGAGTTCCTTATTCTCTAACCCTTGGTTATTCATATTGGGGAGCAGACCATATCCTCAAGCAGATTCTGCCTCCCGAAGTGGAAGTGCCTTCTTCTTTTGAGACAATAGTTAAGTACTCTAGCCACTGCCATGTTTCACCCACTTTTTTCCTTTCTGCCTTGCATTTCCATATGCTCTATATACCTTTTATGTGCGTTCTTTTGTTGCTCTGTCATGTTGCTCACCTGAATATACACGACGAATTGCTTCCATATAAGGATGTCATTGCGAAAGTCATTTATGATAAAAATTATCCGAGAATTAAAACAGTTGTAAATAAAGTGGGGTCTATAACAAATGAGTTTCGGGTTCCAAAGTTTGAAATACTGAAGGGAGAGAATGACATGGTAACAGAAGTGAAGCAATATGGAGCAACTTTCAAGCTTGACTACAGTTTGGTCTACTGGAACTCAAGACTGGAACATGAACACATAAGGCTGGTTTCACTATTCCAACCAGGAGAGATAATTTGTGACATGTTTGCTGGTATTGGTCCGTTTGCTATTCCAGCTGCCCAGAAAGAATGTATTGTATATGCCAATGACTTGAATCCAGACAGTGTTCGATATCTGAAGGTTAATGCAGAAATCAATAAAGTTAGTGGTCGTGTTCATGTATATAATTTGGATGCTAGGAAGTTCATTTCTCAATTGATGACAGTTCCCAGTAATGAAAGTACTTCAGAATCTGTCACGTCAATGTTAAAAACTTGTGAGAAAGGCATCGGAGAGACCAATCAAGAATCACCATCTGCAAATGTTCAGGTAGAAGAAGTCCAAGAGAATATTAAAATTAGCGATGAATGTGGGAGTGGCGATACATCTGTTGCTGCTCTTAAAAGATCCTCAGAAAGTTGTGAGAATGAGAAAGACAATGGTATTGCTGATGATGCTTCCATCTGTGTAGCCGGTAGGAGAAATGGAAATGCTAACAAGAGGATGAAGGGGTCCAATATATCCCATGCTAGGACCTGGGAGCATGTTGATCATGTGGTTATGAACCTCCCTGCTTCTGCTTTACATTTTCTTGATGCATTTAGGGGCACAATTAAGAGGAAGTACTGGAAGGGCTCCCTTCCTTGGATTCATTGTTATTGCTTCATGCGAGCAAATGAAAATCAAGAGTTTATCATTTCGGAGGCTGAGTCTGCCTTGAATGCTAATATTCAAGACCCCATTTTTCATAGGGTCAGAGATGTTGCACCAAACAAGGCAATGTACTGTTTGAGCTTTAGGCTTCCGGAACAATGCGTCAATGAAGACACGATTGGCTGA

Protein sequence

MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEKVQTPDDTIFLSCDSLDGSELSEIPEMKLEELKGLCTIERVPYSLTLGYSYWGADHILKQILPPEVEVPSSFETIVKYSSHCHVSPTFFLSALHFHMLYIPFMCVLLLLCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGPFAIPAAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMTVPSNESTSESVTSMLKTCEKGIGETNQESPSANVQVEEVQENIKISDECGSGDTSVAALKRSSESCENEKDNGIADDASICVAGRRNGNANKRMKGSNISHARTWEHVDHVVMNLPASALHFLDAFRGTIKRKYWKGSLPWIHCYCFMRANENQEFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNEDTIG
Homology
BLAST of Cla97C11G209100 vs. NCBI nr
Match: XP_038890921.1 (tRNA (guanine(37)-N1)-methyltransferase 2 isoform X1 [Benincasa hispida])

HSP 1 Score: 898.7 bits (2321), Expect = 2.5e-257
Identity = 456/536 (85.07%), Postives = 471/536 (87.87%), Query Frame = 0

Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           MLDES+FDVHLNLWALRIPRELCKAAIKILNGYLIDRPRIKPVTEDPT DKNRYVILSEK
Sbjct: 1   MLDESEFDVHLNLWALRIPRELCKAAIKILNGYLIDRPRIKPVTEDPTSDKNRYVILSEK 60

Query: 61  VQTPDDTIFLSCDSLDGSELSEIPEMKLEELKGLCTIERVPYSLTLGYSYWGADHILKQI 120
           VQTP              ELSEIPEM LEELKGLCTI+RVPYSLTLGYSYWGADHILK+I
Sbjct: 61  VQTP--------------ELSEIPEMMLEELKGLCTIDRVPYSLTLGYSYWGADHILKKI 120

Query: 121 LPPEVEVPSSFETIVKYSSHCHVSPTFFLSALHFHMLYIPFMCVLLLLCHVAHLNIHDEL 180
           LPPEVEVPSSFETI                                   HVAHLNIHDEL
Sbjct: 121 LPPEVEVPSSFETI----------------------------------GHVAHLNIHDEL 180

Query: 181 LPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKL 240
           LPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEN+MVTEVKQYGATFKL
Sbjct: 181 LPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENNMVTEVKQYGATFKL 240

Query: 241 DYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGPFAIPAAQKECIVYANDLNPDSVR 300
           DYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGPFAIPAAQK CIVYANDLNPDSVR
Sbjct: 241 DYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGPFAIPAAQKGCIVYANDLNPDSVR 300

Query: 301 YLKVNAEINKVSGRVHVYNLDARKFISQLMTVPSNESTSESVTSMLKTCEKGIGETNQES 360
           YLKVNAEINKVSGRVHVYNLDARKFISQLMTVPSNES SESV S+LK CEKGIGETNQ+S
Sbjct: 301 YLKVNAEINKVSGRVHVYNLDARKFISQLMTVPSNESNSESVISILKACEKGIGETNQKS 360

Query: 361 PSANVQVEEVQENIKISDE----CGSGDTSVAALKRSSESCENEKDNGIADDASICVAGR 420
           PSANVQVEEVQ++ KIS+E      S D SVAALKRSSESCENEKDNGIADDASICVA R
Sbjct: 361 PSANVQVEEVQDDSKISNEYVEHSRSADISVAALKRSSESCENEKDNGIADDASICVASR 420

Query: 421 RNGNANKRMKGSNISHARTWEHVDHVVMNLPASALHFLDAFRGTIKRKYWKGSLPWIHCY 480
           RNGNANKRMKGSNISHARTWEHVDHV+MNLPASALHFLDAFRG+IKRKYWKGSLPWIHCY
Sbjct: 421 RNGNANKRMKGSNISHARTWEHVDHVIMNLPASALHFLDAFRGSIKRKYWKGSLPWIHCY 480

Query: 481 CFMRANENQEFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNED 533
           CFMRANENQ+FIISEAESALNA+IQDP+FHRVRDVAPNKAMYCLSFRLPEQCVN+D
Sbjct: 481 CFMRANENQDFIISEAESALNAHIQDPVFHRVRDVAPNKAMYCLSFRLPEQCVNDD 488

BLAST of Cla97C11G209100 vs. NCBI nr
Match: XP_011648665.1 (tRNA (guanine(37)-N1)-methyltransferase 2 isoform X2 [Cucumis sativus])

HSP 1 Score: 892.9 bits (2306), Expect = 1.4e-255
Identity = 451/537 (83.99%), Postives = 467/537 (86.96%), Query Frame = 0

Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLID+PRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPDDTIFLSCDSLDGSELSEIPEMKLEELKGLCTIERVPYSLTLGYSYWGADHILKQI 120
           VQTP              ELSEIPEMKLEELKGLC  ERVPYSLTLGYSYWGADHILK+I
Sbjct: 61  VQTP--------------ELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKI 120

Query: 121 LPPEVEVPSSFETIVKYSSHCHVSPTFFLSALHFHMLYIPFMCVLLLLCHVAHLNIHDEL 180
           LPPEVEVPSSFETI                                   HVAHLNIHDEL
Sbjct: 121 LPPEVEVPSSFETI----------------------------------GHVAHLNIHDEL 180

Query: 181 LPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKL 240
           LPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKL
Sbjct: 181 LPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKL 240

Query: 241 DYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGPFAIPAAQKECIVYANDLNPDSVR 300
           DYSLVYWNSRLEHEHIRLVSLFQPGE+ICDMFAGIGPFAIPAAQKECIVYANDLNPDS+R
Sbjct: 241 DYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIVYANDLNPDSIR 300

Query: 301 YLKVNAEINKVSGRVHVYNLDARKFISQLMTVPSNESTSESVTSMLKTCEKGIGETNQES 360
           YLKVNAEINKVSGRVHVYNLDARKFISQLM VP NES SESVTS+LK CEKGIG TNQES
Sbjct: 301 YLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILKACEKGIGGTNQES 360

Query: 361 PSANVQVEEVQENIKIS----DECGSGDTSVAALKRSSESCENEKDNGIADDASICVAGR 420
           P ANVQV+EVQ+ IKIS    ++  S D SVA LKRSSESCENEKDNGIADDAS  VAGR
Sbjct: 361 PLANVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENEKDNGIADDASTRVAGR 420

Query: 421 RNGNANKRMKGSNISHARTWEHVDHVVMNLPASALHFLDAFRGTIKRKYWKGSLPWIHCY 480
           RNGN NKRMKGS++SHARTWEHVDHV+MNLPASALHFLDAFRG+IK+KYWKGSLPWIHCY
Sbjct: 421 RNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIKKKYWKGSLPWIHCY 480

Query: 481 CFMRANENQEFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNEDT 534
           CFMRANENQ+FIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCV+EDT
Sbjct: 481 CFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVDEDT 489

BLAST of Cla97C11G209100 vs. NCBI nr
Match: XP_031736378.1 (tRNA (guanine(37)-N1)-methyltransferase 2 isoform X1 [Cucumis sativus])

HSP 1 Score: 887.1 bits (2291), Expect = 7.5e-254
Identity = 451/541 (83.36%), Postives = 467/541 (86.32%), Query Frame = 0

Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLID+PRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPDDTIFLSCDSLDGSELSEIPEMKLEELKGLCTIERVPYSLTLGYSYWGADHILKQI 120
           VQTP              ELSEIPEMKLEELKGLC  ERVPYSLTLGYSYWGADHILK+I
Sbjct: 61  VQTP--------------ELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKI 120

Query: 121 LPPEVEVPSSFETIVKYSSHCHVSPTFFLSALHFHMLYIPFMCVLLLLCHVAHLNIHDEL 180
           LPPEVEVPSSFETI                                   HVAHLNIHDEL
Sbjct: 121 LPPEVEVPSSFETI----------------------------------GHVAHLNIHDEL 180

Query: 181 LPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKL 240
           LPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKL
Sbjct: 181 LPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKL 240

Query: 241 DYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGPFAIPAAQKECIVYANDLNPDSVR 300
           DYSLVYWNSRLEHEHIRLVSLFQPGE+ICDMFAGIGPFAIPAAQKECIVYANDLNPDS+R
Sbjct: 241 DYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIVYANDLNPDSIR 300

Query: 301 YLKVNAEINKVSGRVHVYNLDARKFISQLMTVPSNESTSESVTSMLKTCEKGIGETNQES 360
           YLKVNAEINKVSGRVHVYNLDARKFISQLM VP NES SESVTS+LK CEKGIG TNQES
Sbjct: 301 YLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILKACEKGIGGTNQES 360

Query: 361 PSAN----VQVEEVQENIKIS----DECGSGDTSVAALKRSSESCENEKDNGIADDASIC 420
           P AN    VQV+EVQ+ IKIS    ++  S D SVA LKRSSESCENEKDNGIADDAS  
Sbjct: 361 PLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENEKDNGIADDASTR 420

Query: 421 VAGRRNGNANKRMKGSNISHARTWEHVDHVVMNLPASALHFLDAFRGTIKRKYWKGSLPW 480
           VAGRRNGN NKRMKGS++SHARTWEHVDHV+MNLPASALHFLDAFRG+IK+KYWKGSLPW
Sbjct: 421 VAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIKKKYWKGSLPW 480

Query: 481 IHCYCFMRANENQEFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNED 534
           IHCYCFMRANENQ+FIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCV+ED
Sbjct: 481 IHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVDED 493

BLAST of Cla97C11G209100 vs. NCBI nr
Match: KAA0061573.1 (tRNA (guanine(37)-N1)-methyltransferase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 874.0 bits (2257), Expect = 6.6e-250
Identity = 447/537 (83.24%), Postives = 464/537 (86.41%), Query Frame = 0

Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLID+PRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPDDTIFLSCDSLDGSELSEIPEMKLEELKGLCTIERVPYSLTLGYSYWGADHILKQI 120
           VQTP              ELSEIPEMKLEELKGLC  ERVPYSLTLGYSYWGADHILK+I
Sbjct: 61  VQTP--------------ELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKI 120

Query: 121 LPPEVEVPSSFETIVKYSSHCHVSPTFFLSALHFHMLYIPFMCVLLLLCHVAHLNIHDEL 180
           LPPEVEVPSSFETI            F                VL    HVAHLNIHDEL
Sbjct: 121 LPPEVEVPSSFETI---------GDNF----------------VLCFAGHVAHLNIHDEL 180

Query: 181 LPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKL 240
           LPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKL
Sbjct: 181 LPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKL 240

Query: 241 DYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGPFAIPAAQKECIVYANDLNPDSVR 300
           DYSLVYWNSRLEHEHIRLVSLFQPGE+ICDMFAGIGPFAIPAAQKECIVYANDLNPDSVR
Sbjct: 241 DYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIVYANDLNPDSVR 300

Query: 301 YLKVNAEINKVSGRVHVYNLDARKFISQLMTVPSNESTSESVTSMLKTCEKGIGETNQES 360
           YLKVNAEINKVSGRVHVYNLDARKFISQLM VP NES SE+VTS+LK  E+GIG TNQES
Sbjct: 301 YLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSETVTSILKASEEGIGGTNQES 360

Query: 361 PSANVQVEEVQE---NIKISDECGSGDTSVAALKRSSESCENEKDNGIADDASICVAGRR 420
           P ANVQV+EVQ+   + K  +   S D SVA LKRSSESCENEKD+ IADDAS  VAGRR
Sbjct: 361 PLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSESCENEKDDRIADDASTRVAGRR 420

Query: 421 NGNANKRMKGS-NISHARTWEHVDHVVMNLPASALHFLDAFRGTIKRKYWKGSLPWIHCY 480
           NGN NKRMKGS N+SHARTWEH+DHV+MNLPASALHFLDAFRG+IK+KYWKGSLPWIHCY
Sbjct: 421 NGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAFRGSIKKKYWKGSLPWIHCY 480

Query: 481 CFMRANENQEFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNEDT 534
           CFMRANENQ+FIIS AESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNEDT
Sbjct: 481 CFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNEDT 498

BLAST of Cla97C11G209100 vs. NCBI nr
Match: XP_008458074.1 (PREDICTED: tRNA (guanine(37)-N1)-methyltransferase 2 [Cucumis melo])

HSP 1 Score: 870.5 bits (2248), Expect = 7.3e-249
Identity = 443/537 (82.50%), Postives = 461/537 (85.85%), Query Frame = 0

Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLID+PRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPDDTIFLSCDSLDGSELSEIPEMKLEELKGLCTIERVPYSLTLGYSYWGADHILKQI 120
           VQTP              ELSEIPEMKLEELKGLC  ERVPYSLTLGYSYWGADHILK+I
Sbjct: 61  VQTP--------------ELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKI 120

Query: 121 LPPEVEVPSSFETIVKYSSHCHVSPTFFLSALHFHMLYIPFMCVLLLLCHVAHLNIHDEL 180
           LPPEVEVPSSFETI                                   HVAHLNIHDEL
Sbjct: 121 LPPEVEVPSSFETI----------------------------------GHVAHLNIHDEL 180

Query: 181 LPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKL 240
           LPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKL
Sbjct: 181 LPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKL 240

Query: 241 DYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGPFAIPAAQKECIVYANDLNPDSVR 300
           DYSLVYWNSRLEHEHIRLVSLFQPGE+ICDMFAGIGPFAIPAAQKECIVYANDLNPDSVR
Sbjct: 241 DYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIVYANDLNPDSVR 300

Query: 301 YLKVNAEINKVSGRVHVYNLDARKFISQLMTVPSNESTSESVTSMLKTCEKGIGETNQES 360
           YLKVNAEINKVSGRVHVYNLDARKFIS+LM VP NES SE+VTS+LK  E+GIG TNQES
Sbjct: 301 YLKVNAEINKVSGRVHVYNLDARKFISRLMIVPPNESNSETVTSILKASEEGIGGTNQES 360

Query: 361 PSANVQVEEVQE---NIKISDECGSGDTSVAALKRSSESCENEKDNGIADDASICVAGRR 420
           P ANVQV+EVQ+   + K  +   S D SVA LKRSSESCENEKD+ IADDAS  VAGRR
Sbjct: 361 PLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSESCENEKDDRIADDASTRVAGRR 420

Query: 421 NGNANKRMKGS-NISHARTWEHVDHVVMNLPASALHFLDAFRGTIKRKYWKGSLPWIHCY 480
           NGN NKRMKGS N+SHARTWEH+DHV+MNLPASALHFLDAFRG+IK+KYWKGSLPWIHCY
Sbjct: 421 NGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAFRGSIKKKYWKGSLPWIHCY 480

Query: 481 CFMRANENQEFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNEDT 534
           CFMRANENQ+FIIS AESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNEDT
Sbjct: 481 CFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNEDT 489

BLAST of Cla97C11G209100 vs. ExPASy Swiss-Prot
Match: F6HH45 (tRNA (guanine(37)-N1)-methyltransferase 2 OS=Vitis vinifera OX=29760 GN=VIT_11s0016g04350 PE=3 SV=1)

HSP 1 Score: 669.8 bits (1727), Expect = 2.5e-191
Identity = 343/543 (63.17%), Postives = 401/543 (73.85%), Query Frame = 0

Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           +LDESKFD+ L LWALRIP +LCK A +ILNGYL+D+PR+KP+TEDPTC+ NRY+ILSE+
Sbjct: 2   LLDESKFDLQLKLWALRIPSQLCKVASRILNGYLLDKPRVKPITEDPTCETNRYIILSER 61

Query: 61  VQTPDDTIFLSCDSLDGSELSEIPEMKLEELKGLCTIERVPYSLTLGYSYWGADHILKQI 120
           ++ PD              LS+IPE K +ELK LC IE VPYSLTLGYSYW ADH+LKQI
Sbjct: 62  IKNPD--------------LSDIPEDKRDELKVLCKIEVVPYSLTLGYSYWSADHVLKQI 121

Query: 121 LPPEVEVPSSFETIVKYSSHCHVSPTFFLSALHFHMLYIPFMCVLLLLCHVAHLNIHDEL 180
           LP  VEVPSSFETI                                 LCH+AHLNI  EL
Sbjct: 122 LPDGVEVPSSFETIT------------------------------FSLCHIAHLNITGEL 181

Query: 181 LPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKL 240
           LPYKDVIAKVIYDKNYPRIKTVVNKVG+ITNEFRVPKFEIL G++DMVTEVKQY ATFKL
Sbjct: 182 LPYKDVIAKVIYDKNYPRIKTVVNKVGTITNEFRVPKFEILVGKDDMVTEVKQYRATFKL 241

Query: 241 DYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGPFAIPAAQKECIVYANDLNPDSVR 300
           DYSLVYWNSRLEHEH+RLVS F+PG+IICDMF+G+GPFAIPAAQK C+VYANDLNPDS+R
Sbjct: 242 DYSLVYWNSRLEHEHMRLVSQFRPGQIICDMFSGVGPFAIPAAQKGCLVYANDLNPDSIR 301

Query: 301 YLKVNAEINKVSGRVHVYNLDARKFISQLMTVPSNESTSESVTSMLKTCEKGIGETNQES 360
           YLK+NA+INKV   +  YN+DARKFISQLM VP +E   E    +LK  E+   + N ++
Sbjct: 302 YLKINAKINKVDDNIWAYNMDARKFISQLMEVPVHEILPEPDVPVLKATEECSIQANVQT 361

Query: 361 PSAN---------VQVEEVQENIKISDECGSGDTSVAALKRSSESCENEKDNGIADDASI 420
            S N         V  ++      I   C   D +VAA+K+ S SCE E  NG  D+A++
Sbjct: 362 ESQNGGLTVEAIGVHSDDSSNMDGIEGSCTVADDTVAAVKKHSGSCEEE--NGTYDNATV 421

Query: 421 CVAGRRNGNANKRMKGS-NISHARTWEHVDHVVMNLPASALHFLDAFRGTIKRKYWKGSL 480
            V GRR    NKRM+GS + S+ + WEHVDHV+MNLPASA+ FLDAFRG I+RKYWKGSL
Sbjct: 422 SVVGRRKDRMNKRMRGSESSSNIKPWEHVDHVIMNLPASAIQFLDAFRGLIQRKYWKGSL 481

Query: 481 PWIHCYCFMRANENQEFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQ-CV 533
           PWIHCYCF+RANE  E IISEAE+ALNA+IQ+PI HRVRDVAPNKAM+CLSFRL E+ C 
Sbjct: 482 PWIHCYCFIRANETNEMIISEAETALNASIQEPILHRVRDVAPNKAMFCLSFRLSEEACF 498

BLAST of Cla97C11G209100 vs. ExPASy Swiss-Prot
Match: Q93YU6 (tRNA (guanine(37)-N1)-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=At3g56120 PE=2 SV=1)

HSP 1 Score: 605.1 bits (1559), Expect = 7.5e-172
Identity = 313/536 (58.40%), Postives = 379/536 (70.71%), Query Frame = 0

Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           M DESKFDV+L LWALRIPRELCK+A +ILNGY+++ PRIKP+TEDPTC+K R VILSE 
Sbjct: 1   MFDESKFDVNLKLWALRIPRELCKSASRILNGYMLNMPRIKPITEDPTCEKTRLVILSES 60

Query: 61  VQTPDDTIFLSCDSLDGSELSEIPEMKLEELKGLCTIERVPYSLTLGYSYWGADHILKQI 120
           V+  D              LSEIPE KL +LK L  +E VP+S+TLGYSYW ADH+LKQI
Sbjct: 61  VKNAD--------------LSEIPEEKLNQLKKLSELEVVPHSVTLGYSYWSADHLLKQI 120

Query: 121 LPPEVEVPSSFETIVKYSSHCHVSPTFFLSALHFHMLYIPFMCVLLLLCHVAHLNIHDEL 180
           LP  +++PSSFETI                                   H+AHLN+HDEL
Sbjct: 121 LPDGLDIPSSFETI----------------------------------GHIAHLNLHDEL 180

Query: 181 LPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKL 240
           LP+KDVIAKVIYDKNYPRIKT+VNKVG+I+NEFRVPKFE+L GEN M TEVKQYGA FKL
Sbjct: 181 LPFKDVIAKVIYDKNYPRIKTIVNKVGTISNEFRVPKFEVLAGENGMETEVKQYGARFKL 240

Query: 241 DYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGPFAIPAAQKECIVYANDLNPDSVR 300
           DY LVYWNSRLEHEH+RL SLF+PGE +CDMFAGIGPFAIPAAQK C VYANDLNPDSVR
Sbjct: 241 DYGLVYWNSRLEHEHMRLSSLFKPGETVCDMFAGIGPFAIPAAQKGCFVYANDLNPDSVR 300

Query: 301 YLKVNAEINKVSGRVHVYNLDARKFISQLMTVPSNESTSESVTSMLKTCEKGI---GETN 360
           YLK+NA+ NKV   + V+N+DARKF S LM V + E   +SV    KT E  +   GETN
Sbjct: 301 YLKINAKFNKVDDLICVHNMDARKFFSHLMAVSTCEDNLQSVADNDKTKEAAVSRGGETN 360

Query: 361 QESPSANVQVEEVQE-NIKISDECGSGDTSVAALKRSSESCENEKDNGIADDASICVAGR 420
                     EE++E N  I++  G       A K+ S + + E  NG+  D        
Sbjct: 361 SSG-------EEIRESNASINEPLG-------ANKKPSGTTKTE--NGVGKDCKSI---- 420

Query: 421 RNGNANKRMKGSNISHARTWEHVDHVVMNLPASALHFLDAFRGTIKRKYWKGSLPWIHCY 480
             G+ANKR++ + +  A+ WEH+DHV+MNLPASAL FLD+F   I++KYWKG LP IHCY
Sbjct: 421 -EGHANKRLRQTLLPIAKPWEHIDHVIMNLPASALQFLDSFSNVIQKKYWKGPLPLIHCY 467

Query: 481 CFMRANENQEFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNED 533
           CF+RA+E  EFII+EAE+AL  +I+DP+FH+VRDVAPNKAM+CLSFRLPE C+ ++
Sbjct: 481 CFIRASETTEFIIAEAETALKFHIEDPVFHKVRDVAPNKAMFCLSFRLPEACLKQE 467

BLAST of Cla97C11G209100 vs. ExPASy Swiss-Prot
Match: C5XX79 (tRNA (guanine(37)-N1)-methyltransferase OS=Sorghum bicolor OX=4558 GN=Sb04g025390 PE=3 SV=1)

HSP 1 Score: 540.4 bits (1391), Expect = 2.3e-152
Identity = 286/533 (53.66%), Postives = 356/533 (66.79%), Query Frame = 0

Query: 2   LDESKFDVHLNLWALRIPRELCKAAIKIL-NGYLIDRPRIKPVTEDPTCDKNRYVILSEK 61
           LDESKF+  L LWALRIPREL  A  ++L +GYL+D+PR+K V EDP  DKNR VILSEK
Sbjct: 4   LDESKFEQRLQLWALRIPRELSSAVTRLLRSGYLLDKPRVKTVVEDPEGDKNRLVILSEK 63

Query: 62  VQTPDDTIFLSCDSLDGSELSEIPEMKLEELKGLCTIERVPYSLTLGYSYWGADHILKQI 121
           +Q PD              LS++P+ +L+ LK LC ++ VPY+LTLGYSYW A       
Sbjct: 64  IQKPD--------------LSDMPQQELDSLKQLCNVDVVPYTLTLGYSYWSAG------ 123

Query: 122 LPPEVEVPSSFETIVKYSSHCHVSPTFFLSALHFHMLYIPFMCVLLLLCHVAHLNIHDEL 181
                                                            HVAHLNI D+L
Sbjct: 124 -------------------------------------------------HVAHLNISDDL 183

Query: 182 LPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKL 241
           LPYK+VIAKVIYDKNYPRI+TV NKVG+ITNEFRVPKFEIL G+NDMVTEVKQYGATFKL
Sbjct: 184 LPYKNVIAKVIYDKNYPRIQTVANKVGTITNEFRVPKFEILAGKNDMVTEVKQYGATFKL 243

Query: 242 DYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGPFAIPAAQKECIVYANDLNPDSVR 301
           DY LVYWNSRL+HEHIRLVSLF+ G++ICDMFAGIGPFAIPAAQK C+VYANDLNPDSV 
Sbjct: 244 DYGLVYWNSRLDHEHIRLVSLFKKGDVICDMFAGIGPFAIPAAQKGCVVYANDLNPDSVH 303

Query: 302 YLKVNAEINKVSGRVHVYNLDARKFISQLMTVPSNESTSESVTSMLKTCEKGIGETNQES 361
           YL+ NA+INKV   +  YN+DAR F+  LMTVP  E+ S+   +  ++  K  G    E+
Sbjct: 304 YLRTNAKINKVDDYIFAYNMDARVFMQNLMTVPGLETGSDCQVAADESYPKE-GVPANEN 363

Query: 362 PSANVQVEEVQENIKI-SDECGSGDTSVAALKRSSESCENEKDNGIADDASICVAGRRNG 421
            S+N    +V+E  +  ++E     T+    +++SE CE++  +G A         RRN 
Sbjct: 364 SSSNGNHNDVREGSQNGANESSVASTTAKKRQQTSEECESDCQDGDASQTK-----RRN- 423

Query: 422 NANKRMKGSNISHARTWEHVDHVVMNLPASALHFLDAFRGTIKRKYWKGSLPWIHCYCFM 481
             NKR++G     ++ WEH DHV+MNLPASAL FLD F G +++KYW GSLPWIHCYCF+
Sbjct: 424 --NKRVRGPGPPPSKPWEHFDHVLMNLPASALQFLDCFDGLVQKKYWTGSLPWIHCYCFI 455

Query: 482 RANENQEFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNED 533
           R++E++E I+S   + LNA I +PIFHRVRDVAPNKAM+CLSF+LP +C+ ED
Sbjct: 484 RSSESEESILS---NKLNAKIAEPIFHRVRDVAPNKAMFCLSFKLPMECLRED 455

BLAST of Cla97C11G209100 vs. ExPASy Swiss-Prot
Match: A9T6G5 (tRNA (guanine(37)-N1)-methyltransferase OS=Physcomitrium patens OX=3218 GN=PHYPADRAFT_141055 PE=3 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 4.5e-108
Identity = 221/515 (42.91%), Postives = 294/515 (57.09%), Query Frame = 0

Query: 2   LDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEKV 61
           +D+S F V L   ALRIP++       +L+ YL+D PR+K + EDP     R ++ SE +
Sbjct: 11  IDKSAFVVRLPRVALRIPKQKSHRVASLLSKYLLDMPRVKHIVEDPQDPSMRLLLFSEAI 70

Query: 62  QTPDDTIFLSCDSLDGSELSEIPEMKLEELKGLCTIERVPYSLTLGYSYWGADHILKQIL 121
             PD              L  + E K+  +K +  ++ V + + L YSYW  +HILK+IL
Sbjct: 71  AQPD--------------LEGVSEEKILAVKEIVPLDVVQHEVVLDYSYWPVEHILKEIL 130

Query: 122 PPEVEVPSSFETIVKYSSHCHVSPTFFLSALHFHMLYIPFMCVLLLLCHVAHLNIHDELL 181
           P   EVPSSFETI                                   H+AHLN+ ++LL
Sbjct: 131 PAGCEVPSSFETI----------------------------------GHIAHLNLREDLL 190

Query: 182 PYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKLD 241
            YK +IA+VI DKN P++KTVVNKVG+ITNEFRVP+FEIL GE  +VTE+KQ+GATF+LD
Sbjct: 191 TYKKIIAEVILDKN-PKLKTVVNKVGTITNEFRVPEFEILAGEPSLVTEIKQHGATFRLD 250

Query: 242 YSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGPFAIPAAQKECIVYANDLNPDSVRY 301
           Y +VYWNSRLE EH RL + F+PG++I DMFAGIGPFAIPAAQ+ C VYANDLNP SV++
Sbjct: 251 YGMVYWNSRLEGEHKRLFAQFKPGQVIVDMFAGIGPFAIPAAQQGCAVYANDLNPTSVKF 310

Query: 302 LKVNAEINKVSGRVHVYNLDARKFISQLMTVPSNESTSESVTSMLKTCEKGIGETNQESP 361
           LK+N++INKV   +  +NLDAR+F+ +L+T    E     + S     E  +  T   S 
Sbjct: 311 LKLNSDINKVGESIKAFNLDAREFMRKLVT--EEEGCVGEIASSTLIAENSLNGTRIPSS 370

Query: 362 SANVQVEEVQENIKISDECGSGDTSVAALKRSSESCENEKDNGIADDASICVAGRRNGNA 421
             N            S     G    ++ K      +  K + +                
Sbjct: 371 KEN------------SSSLTEGSDMPSSKKPEERKAKKAKVSEVV--------------- 430

Query: 422 NKRMKGSNISHARTWEHVDHVVMNLPASALHFLDAFRGTIKRKYWKGSLPWIHCYCFMRA 481
             +MK   IS  + WEH DHVVMNLPASAL FLD   G + +  WKG++P +HCYCFMR+
Sbjct: 431 --KMK---ISDVKPWEHFDHVVMNLPASALEFLDVLNGLLCKGRWKGTMPRVHCYCFMRS 442

Query: 482 NENQEFIISEAESALNANIQDPIFHRVRDVAPNKA 517
           NE    I+ +AE+ L  +I DP  + VRDVAPNKA
Sbjct: 491 NETNADIVKKAETYLGGSIADPDVYVVRDVAPNKA 442

BLAST of Cla97C11G209100 vs. ExPASy Swiss-Prot
Match: F4NUJ6 (tRNA (guanine(37)-N1)-methyltransferase OS=Batrachochytrium dendrobatidis (strain JAM81 / FGSC 10211) OX=684364 GN=TRM5 PE=3 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 3.3e-71
Identity = 171/530 (32.26%), Postives = 262/530 (49.43%), Query Frame = 0

Query: 2   LDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEKV 61
           L+ + F+    L AL+IP      A++ L  +++  PR++ + +DPT  K R ++L    
Sbjct: 16  LNHALFESSHTLPALKIPAHASGIAMETLRSHILVAPRLRTIVDDPTDKKWRLLLLD--- 75

Query: 62  QTPDDTIFLSCDSLDGSELSEIPEMKLEELKGLCTIERVPYSLTLGYSYWGADHILKQIL 121
                       S++ SE++++P   L+E       + V +++ L Y YW +D +L+ IL
Sbjct: 76  -----------PSMEASEINDLPN-PLKEFALKHEAKLVKHTIELKYDYWTSDQVLRSIL 135

Query: 122 PPEVEVPSSFETIVKYSSHCHVSPTFFLSALHFHMLYIPFMCVLLLLCHVAHLNIHDELL 181
           P E+E P +FET+                                   H+AHLN+ D   
Sbjct: 136 PDEMETPGAFETV----------------------------------GHIAHLNLRDRYQ 195

Query: 182 PYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKLD 241
           P+K +I +VI DK+   IKTVVNK+ +I + FR  + EIL G NDM  ++K+ G  F  D
Sbjct: 196 PFKHIIGQVILDKS-SHIKTVVNKLDNIDHTFRFFQMEILAGINDMNAKLKEGGCFFHFD 255

Query: 242 YSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGPFAIPAAQ-KECIVYANDLNPDSVR 301
           +S VYWNSRL+ EH R++ LF   ++ICD+FAG+GPFA+PAA+ K C+V+ANDLNP S +
Sbjct: 256 FSKVYWNSRLQGEHDRIIKLFGQNDLICDVFAGVGPFALPAAKHKRCVVFANDLNPQSFK 315

Query: 302 YLKVNAEINKVSGRVHVYNLDARKFISQLMTVPSNESTSESVTSMLKTCEKGIGETNQES 361
           YL  N ++NK+  R+  +N+D R+FI Q +   +N +    +T              ++ 
Sbjct: 316 YLMENIKLNKLETRILPFNMDGRQFIKQSLEDLNNPAIWNKIT--------------KQK 375

Query: 362 PSANVQVEEVQENIKISDECGSGDTSVAALKRSSESCENEKDNGIADDASICVAGRRNGN 421
           P++N    + + N K+     +  T   A+            +GI               
Sbjct: 376 PTSN----DKKRNRKVESPTVAPLTDQPAI------------SGI--------------- 434

Query: 422 ANKRMKGSNISHARTWEHVDHVVMNLPASALHFLDAFRGT---IKRKYWKGSLPWIHCYC 481
                            H  H VMNLPA+A+ FLDAF G    ++       LP IHC+C
Sbjct: 436 ----------------RHFKHYVMNLPATAIEFLDAFHGLYSGMRDVIMDSDLPTIHCHC 434

Query: 482 FMRANENQEFIISEAESALNANIQDPIF--HRVRDVAPNKAMYCLSFRLP 526
           F  A + +  +I   E  +   +   +   H VR VAPNK M C+SFRLP
Sbjct: 496 FSNAKDVKADVIERVERVIGMPLGSNLIMVHSVRTVAPNKDMLCISFRLP 434

BLAST of Cla97C11G209100 vs. ExPASy TrEMBL
Match: A0A0A0LFW8 (tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_2G005350 PE=3 SV=1)

HSP 1 Score: 900.6 bits (2326), Expect = 3.2e-258
Identity = 454/537 (84.54%), Postives = 470/537 (87.52%), Query Frame = 0

Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLID+PRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPDDTIFLSCDSLDGSELSEIPEMKLEELKGLCTIERVPYSLTLGYSYWGADHILKQI 120
           VQTP              ELSEIPEMKLEELKGLC  ERVPYSLTLGYSYWGADHILK+I
Sbjct: 61  VQTP--------------ELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKI 120

Query: 121 LPPEVEVPSSFETIVKYSSHCHVSPTFFLSALHFHMLYIPFMCVLLLLCHVAHLNIHDEL 180
           LPPEVEVPSSFETI                                LLCHVAHLNIHDEL
Sbjct: 121 LPPEVEVPSSFETI--------------------------------LLCHVAHLNIHDEL 180

Query: 181 LPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKL 240
           LPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKL
Sbjct: 181 LPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKL 240

Query: 241 DYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGPFAIPAAQKECIVYANDLNPDSVR 300
           DYSLVYWNSRLEHEHIRLVSLFQPGE+ICDMFAGIGPFAIPAAQKECIVYANDLNPDS+R
Sbjct: 241 DYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIVYANDLNPDSIR 300

Query: 301 YLKVNAEINKVSGRVHVYNLDARKFISQLMTVPSNESTSESVTSMLKTCEKGIGETNQES 360
           YLKVNAEINKVSGRVHVYNLDARKFISQLM VP NES SESVTS+LK CEKGIG TNQES
Sbjct: 301 YLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILKACEKGIGGTNQES 360

Query: 361 PSANVQVEEVQENIKIS----DECGSGDTSVAALKRSSESCENEKDNGIADDASICVAGR 420
           P ANVQV+EVQ+ IKIS    ++  S D SVA LKRSSESCENEKDNGIADDAS  VAGR
Sbjct: 361 PLANVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENEKDNGIADDASTRVAGR 420

Query: 421 RNGNANKRMKGSNISHARTWEHVDHVVMNLPASALHFLDAFRGTIKRKYWKGSLPWIHCY 480
           RNGN NKRMKGS++SHARTWEHVDHV+MNLPASALHFLDAFRG+IK+KYWKGSLPWIHCY
Sbjct: 421 RNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIKKKYWKGSLPWIHCY 480

Query: 481 CFMRANENQEFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNEDT 534
           CFMRANENQ+FIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCV+EDT
Sbjct: 481 CFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVDEDT 491

BLAST of Cla97C11G209100 vs. ExPASy TrEMBL
Match: A0A5A7V0B1 (tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold41G001350 PE=3 SV=1)

HSP 1 Score: 874.0 bits (2257), Expect = 3.2e-250
Identity = 447/537 (83.24%), Postives = 464/537 (86.41%), Query Frame = 0

Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLID+PRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPDDTIFLSCDSLDGSELSEIPEMKLEELKGLCTIERVPYSLTLGYSYWGADHILKQI 120
           VQTP              ELSEIPEMKLEELKGLC  ERVPYSLTLGYSYWGADHILK+I
Sbjct: 61  VQTP--------------ELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKI 120

Query: 121 LPPEVEVPSSFETIVKYSSHCHVSPTFFLSALHFHMLYIPFMCVLLLLCHVAHLNIHDEL 180
           LPPEVEVPSSFETI            F                VL    HVAHLNIHDEL
Sbjct: 121 LPPEVEVPSSFETI---------GDNF----------------VLCFAGHVAHLNIHDEL 180

Query: 181 LPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKL 240
           LPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKL
Sbjct: 181 LPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKL 240

Query: 241 DYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGPFAIPAAQKECIVYANDLNPDSVR 300
           DYSLVYWNSRLEHEHIRLVSLFQPGE+ICDMFAGIGPFAIPAAQKECIVYANDLNPDSVR
Sbjct: 241 DYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIVYANDLNPDSVR 300

Query: 301 YLKVNAEINKVSGRVHVYNLDARKFISQLMTVPSNESTSESVTSMLKTCEKGIGETNQES 360
           YLKVNAEINKVSGRVHVYNLDARKFISQLM VP NES SE+VTS+LK  E+GIG TNQES
Sbjct: 301 YLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSETVTSILKASEEGIGGTNQES 360

Query: 361 PSANVQVEEVQE---NIKISDECGSGDTSVAALKRSSESCENEKDNGIADDASICVAGRR 420
           P ANVQV+EVQ+   + K  +   S D SVA LKRSSESCENEKD+ IADDAS  VAGRR
Sbjct: 361 PLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSESCENEKDDRIADDASTRVAGRR 420

Query: 421 NGNANKRMKGS-NISHARTWEHVDHVVMNLPASALHFLDAFRGTIKRKYWKGSLPWIHCY 480
           NGN NKRMKGS N+SHARTWEH+DHV+MNLPASALHFLDAFRG+IK+KYWKGSLPWIHCY
Sbjct: 421 NGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAFRGSIKKKYWKGSLPWIHCY 480

Query: 481 CFMRANENQEFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNEDT 534
           CFMRANENQ+FIIS AESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNEDT
Sbjct: 481 CFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNEDT 498

BLAST of Cla97C11G209100 vs. ExPASy TrEMBL
Match: A0A1S3C7L3 (tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103497610 PE=3 SV=1)

HSP 1 Score: 870.5 bits (2248), Expect = 3.5e-249
Identity = 443/537 (82.50%), Postives = 461/537 (85.85%), Query Frame = 0

Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLID+PRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPDDTIFLSCDSLDGSELSEIPEMKLEELKGLCTIERVPYSLTLGYSYWGADHILKQI 120
           VQTP              ELSEIPEMKLEELKGLC  ERVPYSLTLGYSYWGADHILK+I
Sbjct: 61  VQTP--------------ELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKI 120

Query: 121 LPPEVEVPSSFETIVKYSSHCHVSPTFFLSALHFHMLYIPFMCVLLLLCHVAHLNIHDEL 180
           LPPEVEVPSSFETI                                   HVAHLNIHDEL
Sbjct: 121 LPPEVEVPSSFETI----------------------------------GHVAHLNIHDEL 180

Query: 181 LPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKL 240
           LPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKL
Sbjct: 181 LPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKL 240

Query: 241 DYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGPFAIPAAQKECIVYANDLNPDSVR 300
           DYSLVYWNSRLEHEHIRLVSLFQPGE+ICDMFAGIGPFAIPAAQKECIVYANDLNPDSVR
Sbjct: 241 DYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIVYANDLNPDSVR 300

Query: 301 YLKVNAEINKVSGRVHVYNLDARKFISQLMTVPSNESTSESVTSMLKTCEKGIGETNQES 360
           YLKVNAEINKVSGRVHVYNLDARKFIS+LM VP NES SE+VTS+LK  E+GIG TNQES
Sbjct: 301 YLKVNAEINKVSGRVHVYNLDARKFISRLMIVPPNESNSETVTSILKASEEGIGGTNQES 360

Query: 361 PSANVQVEEVQE---NIKISDECGSGDTSVAALKRSSESCENEKDNGIADDASICVAGRR 420
           P ANVQV+EVQ+   + K  +   S D SVA LKRSSESCENEKD+ IADDAS  VAGRR
Sbjct: 361 PLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSESCENEKDDRIADDASTRVAGRR 420

Query: 421 NGNANKRMKGS-NISHARTWEHVDHVVMNLPASALHFLDAFRGTIKRKYWKGSLPWIHCY 480
           NGN NKRMKGS N+SHARTWEH+DHV+MNLPASALHFLDAFRG+IK+KYWKGSLPWIHCY
Sbjct: 421 NGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAFRGSIKKKYWKGSLPWIHCY 480

Query: 481 CFMRANENQEFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNEDT 534
           CFMRANENQ+FIIS AESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNEDT
Sbjct: 481 CFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNEDT 489

BLAST of Cla97C11G209100 vs. ExPASy TrEMBL
Match: A0A6J1DUW2 (tRNA (guanine(37)-N1)-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111024249 PE=3 SV=1)

HSP 1 Score: 845.5 bits (2183), Expect = 1.2e-241
Identity = 430/535 (80.37%), Postives = 449/535 (83.93%), Query Frame = 0

Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHL LWALRIPRELCKAAIKILNGYLIDR R+KPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLKLWALRIPRELCKAAIKILNGYLIDRARVKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPDDTIFLSCDSLDGSELSEIPEMKLEELKGLCTIERVPYSLTLGYSYWGADHILKQI 120
           VQ P              ELS+IPE KLEELKGLC IE VPYSLTLGYSYWGADHILKQI
Sbjct: 61  VQAP--------------ELSQIPEAKLEELKGLCEIELVPYSLTLGYSYWGADHILKQI 120

Query: 121 LPPEVEVPSSFETIVKYSSHCHVSPTFFLSALHFHMLYIPFMCVLLLLCHVAHLNIHDEL 180
           LPP VEVPSSFETI                                   H+AHLNIHDEL
Sbjct: 121 LPPRVEVPSSFETI----------------------------------GHIAHLNIHDEL 180

Query: 181 LPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKL 240
           LPYKDVIAKVIYDKNYPRIKTVVNKVG+ITN+FRVPKFEIL GE+DMVTEVKQYGATFKL
Sbjct: 181 LPYKDVIAKVIYDKNYPRIKTVVNKVGTITNKFRVPKFEILMGEHDMVTEVKQYGATFKL 240

Query: 241 DYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGPFAIPAAQKECIVYANDLNPDSVR 300
           DY LVYWNSRLEHEH+RLVSLFQPGEIICDMFAGIGPFAIPAAQKECIVY NDLNPDSV+
Sbjct: 241 DYGLVYWNSRLEHEHVRLVSLFQPGEIICDMFAGIGPFAIPAAQKECIVYGNDLNPDSVQ 300

Query: 301 YLKVNAEINKVSGRVHVYNLDARKFISQLMTVPSNESTSESVTSMLKTCEKGIGETNQES 360
           YLKVNAEINKVS RVHVYNLDARKFISQLM VP NES SESVTS+LK C+KGIG +NQES
Sbjct: 301 YLKVNAEINKVSDRVHVYNLDARKFISQLMIVPDNESNSESVTSVLKACDKGIGGSNQES 360

Query: 361 PSANVQVEEVQENIKISDE----CGSGDTSVAALKRSSESCENEKDNGIADDASICVAGR 420
           PSANVQVEEVQEN KISDE      S D SV+ LKRSSESC  EKD+GI DDASI VAGR
Sbjct: 361 PSANVQVEEVQENSKISDETVEHSRSADISVSVLKRSSESC--EKDDGIVDDASISVAGR 420

Query: 421 RNGNANKRMKGSNISHARTWEHVDHVVMNLPASALHFLDAFRGTIKRKYWKGSLPWIHCY 480
           +  NANKRMKGSNI HARTWEHVDHV+MNLPASAL FLDAFRG+I+RKYWKGSLPWIHCY
Sbjct: 421 KKRNANKRMKGSNICHARTWEHVDHVIMNLPASALQFLDAFRGSIRRKYWKGSLPWIHCY 480

Query: 481 CFMRANENQEFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNE 532
           CFMRANENQ+FIISEAESALN  IQDP+FHRVRDVAPNKAMYCLSFRLPEQC NE
Sbjct: 481 CFMRANENQDFIISEAESALNGKIQDPVFHRVRDVAPNKAMYCLSFRLPEQCFNE 485

BLAST of Cla97C11G209100 vs. ExPASy TrEMBL
Match: A0A5D3CFY1 (tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G00100 PE=3 SV=1)

HSP 1 Score: 823.2 bits (2125), Expect = 6.5e-235
Identity = 423/537 (78.77%), Postives = 440/537 (81.94%), Query Frame = 0

Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLID+PRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPDDTIFLSCDSLDGSELSEIPEMKLEELKGLCTIERVPYSLTLGYSYWGADHILKQI 120
           VQTP              ELSEIPEMKLEELKGLC  ERVPYSLTLGYSYWGA       
Sbjct: 61  VQTP--------------ELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGAG------ 120

Query: 121 LPPEVEVPSSFETIVKYSSHCHVSPTFFLSALHFHMLYIPFMCVLLLLCHVAHLNIHDEL 180
                                                            HVAHLNIHDEL
Sbjct: 121 -------------------------------------------------HVAHLNIHDEL 180

Query: 181 LPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKL 240
           LPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKL
Sbjct: 181 LPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKL 240

Query: 241 DYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGPFAIPAAQKECIVYANDLNPDSVR 300
           DYSLVYWNSRLEHEHIRLVSLFQPGE+ICDMFAGIGPFAIPAAQKECIVYANDLNPDSVR
Sbjct: 241 DYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIVYANDLNPDSVR 300

Query: 301 YLKVNAEINKVSGRVHVYNLDARKFISQLMTVPSNESTSESVTSMLKTCEKGIGETNQES 360
           YLKVNAEINKVSGRVHVYNLDARKFISQLM VP NES SE+VTS+LK  E+GIG TNQES
Sbjct: 301 YLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSETVTSILKASEEGIGGTNQES 360

Query: 361 PSANVQVEEVQE---NIKISDECGSGDTSVAALKRSSESCENEKDNGIADDASICVAGRR 420
           P ANVQV+EVQ+   + K  +   S D SVA LKRSSESCENEK++ IADDAS  VAGRR
Sbjct: 361 PLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSESCENEKEDRIADDASTRVAGRR 420

Query: 421 NGNANKRMKGS-NISHARTWEHVDHVVMNLPASALHFLDAFRGTIKRKYWKGSLPWIHCY 480
           NGN NKRMKGS N+SHARTWEH+DHV+MNLPASALHFLDAFRG+IK+KYWKGSLPWIHCY
Sbjct: 421 NGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAFRGSIKKKYWKGSLPWIHCY 468

Query: 481 CFMRANENQEFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNEDT 534
           CFMRANENQ+FIIS AESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNEDT
Sbjct: 481 CFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNEDT 468

BLAST of Cla97C11G209100 vs. TAIR 10
Match: AT3G56120.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 605.1 bits (1559), Expect = 5.3e-173
Identity = 313/536 (58.40%), Postives = 379/536 (70.71%), Query Frame = 0

Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           M DESKFDV+L LWALRIPRELCK+A +ILNGY+++ PRIKP+TEDPTC+K R VILSE 
Sbjct: 1   MFDESKFDVNLKLWALRIPRELCKSASRILNGYMLNMPRIKPITEDPTCEKTRLVILSES 60

Query: 61  VQTPDDTIFLSCDSLDGSELSEIPEMKLEELKGLCTIERVPYSLTLGYSYWGADHILKQI 120
           V+  D              LSEIPE KL +LK L  +E VP+S+TLGYSYW ADH+LKQI
Sbjct: 61  VKNAD--------------LSEIPEEKLNQLKKLSELEVVPHSVTLGYSYWSADHLLKQI 120

Query: 121 LPPEVEVPSSFETIVKYSSHCHVSPTFFLSALHFHMLYIPFMCVLLLLCHVAHLNIHDEL 180
           LP  +++PSSFETI                                   H+AHLN+HDEL
Sbjct: 121 LPDGLDIPSSFETI----------------------------------GHIAHLNLHDEL 180

Query: 181 LPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKL 240
           LP+KDVIAKVIYDKNYPRIKT+VNKVG+I+NEFRVPKFE+L GEN M TEVKQYGA FKL
Sbjct: 181 LPFKDVIAKVIYDKNYPRIKTIVNKVGTISNEFRVPKFEVLAGENGMETEVKQYGARFKL 240

Query: 241 DYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGPFAIPAAQKECIVYANDLNPDSVR 300
           DY LVYWNSRLEHEH+RL SLF+PGE +CDMFAGIGPFAIPAAQK C VYANDLNPDSVR
Sbjct: 241 DYGLVYWNSRLEHEHMRLSSLFKPGETVCDMFAGIGPFAIPAAQKGCFVYANDLNPDSVR 300

Query: 301 YLKVNAEINKVSGRVHVYNLDARKFISQLMTVPSNESTSESVTSMLKTCEKGI---GETN 360
           YLK+NA+ NKV   + V+N+DARKF S LM V + E   +SV    KT E  +   GETN
Sbjct: 301 YLKINAKFNKVDDLICVHNMDARKFFSHLMAVSTCEDNLQSVADNDKTKEAAVSRGGETN 360

Query: 361 QESPSANVQVEEVQE-NIKISDECGSGDTSVAALKRSSESCENEKDNGIADDASICVAGR 420
                     EE++E N  I++  G       A K+ S + + E  NG+  D        
Sbjct: 361 SSG-------EEIRESNASINEPLG-------ANKKPSGTTKTE--NGVGKDCKSI---- 420

Query: 421 RNGNANKRMKGSNISHARTWEHVDHVVMNLPASALHFLDAFRGTIKRKYWKGSLPWIHCY 480
             G+ANKR++ + +  A+ WEH+DHV+MNLPASAL FLD+F   I++KYWKG LP IHCY
Sbjct: 421 -EGHANKRLRQTLLPIAKPWEHIDHVIMNLPASALQFLDSFSNVIQKKYWKGPLPLIHCY 467

Query: 481 CFMRANENQEFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNED 533
           CF+RA+E  EFII+EAE+AL  +I+DP+FH+VRDVAPNKAM+CLSFRLPE C+ ++
Sbjct: 481 CFIRASETTEFIIAEAETALKFHIEDPVFHKVRDVAPNKAMFCLSFRLPEACLKQE 467

BLAST of Cla97C11G209100 vs. TAIR 10
Match: AT4G27340.1 (Met-10+ like family protein )

HSP 1 Score: 196.8 bits (499), Expect = 4.4e-50
Identity = 137/467 (29.34%), Postives = 199/467 (42.61%), Query Frame = 0

Query: 68  IFLSCDSLDGSELSEIPE------MKLEELKGLCTIERVPYSLTLGYSYWGADHILKQIL 127
           + L  +   G E+ ++PE       + +      + E V   LTL Y YW    IL+ +L
Sbjct: 275 LLLLDEKYSGEEVQDLPEAIKVLFAEAKMADASLSFELVKCRLTLFYDYWPMIEILEAVL 334

Query: 128 PPEVEVPSSFETIVKYSSHCHVSPTFFLSALHFHMLYIPFMCVLLLLCHVAHLNIHDELL 187
           P  + VPS+FE                                  ++ H+AHLN+ DE L
Sbjct: 335 PKGMIVPSAFE----------------------------------MVGHIAHLNLRDEHL 394

Query: 188 PYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKLD 247
           PYK +IAKV+ DKN P+I+TVVNK+  I N+FR  + E+L G + +VT V + G  F +D
Sbjct: 395 PYKRLIAKVVLDKNQPKIQTVVNKIDPIHNDFRTMQLEVLAGNHSLVTLVVENGLRFHVD 454

Query: 248 YSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGPFAIPAAQKECIVYANDLNPDSVRY 307
            + VYWNS+L  E  RL+  F   +++CD+FAG+GP A+ AA+    VYANDLNP +V +
Sbjct: 455 LARVYWNSKLGTERQRLLLGFDQNDVVCDVFAGVGPIALAAARIVKRVYANDLNPHAVEF 514

Query: 308 LKVNAEINKVSGRVHVYNLDARKFISQLMTVPSNESTSESVTSMLKTCEKGIGETNQESP 367
           ++ N+ +NK+  R+ ++N+D R+FI  +                  + EKG         
Sbjct: 515 MEQNSVVNKLEKRIEIFNMDGRRFIKAMF-----------------SSEKG--------- 574

Query: 368 SANVQVEEVQENIKISDECGSGDTSVAALKRSSESCENEKDNGIADDASICVAGRRNGNA 427
                                                                       
Sbjct: 575 ------------------------------------------------------------ 606

Query: 428 NKRMKGSNISHARTWEHVDHVVMNLPASALHFLDAFRGTIKRKYWKG--SLPWIHCYCFM 487
                          + V  VVMNLP  A   LDAFRG    ++     S P IH Y F 
Sbjct: 635 ---------------QKVTQVVMNLPKDAAESLDAFRGVYNDRHRDEGLSFPTIHVYGFS 606

Query: 488 RANENQEFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPE 527
           +A++ +         AL+    D    +VR VAP K M C SF LP+
Sbjct: 695 KASDPEFDFHERIRIALSEVAVDVKMRKVRLVAPGKWMLCASFILPK 606

BLAST of Cla97C11G209100 vs. TAIR 10
Match: AT4G04670.1 (Met-10+ like family protein / kelch repeat-containing protein )

HSP 1 Score: 55.1 bits (131), Expect = 2.0e-07
Identity = 49/176 (27.84%), Postives = 80/176 (45.45%), Query Frame = 0

Query: 211 NEFRVPKFEILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICD 270
           N  R    EIL G+N  V   ++ G  +  D +   ++     E +R+ ++    E++ D
Sbjct: 785 NGTRDSTLEILVGDNGWVNH-RENGILYSFDATKCMFSWGNLSEKLRMGNMACENEVVVD 844

Query: 271 MFAGIGPFAIP--AAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQ 330
           +FAGIG F +P     K  +VYA + NP ++  L+ N E N VS R  +   D R     
Sbjct: 845 LFAGIGYFVLPFLVRAKAKLVYACEWNPHAIEALRRNVEANSVSERCIILEGDNR----- 904

Query: 331 LMTVPSNESTSESVTSMLKTCEKGIGETNQESPSANVQVEEVQENIKISDECGSGD 385
            +T P  +  ++ V   L    +G   T  ++      +  V  N+K SDE   G+
Sbjct: 905 -ITAP--KGVADRVNLGLIPSSEGSWVTAIQALRPEGGILHVHGNVKDSDESSWGE 951

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038890921.12.5e-25785.07tRNA (guanine(37)-N1)-methyltransferase 2 isoform X1 [Benincasa hispida][more]
XP_011648665.11.4e-25583.99tRNA (guanine(37)-N1)-methyltransferase 2 isoform X2 [Cucumis sativus][more]
XP_031736378.17.5e-25483.36tRNA (guanine(37)-N1)-methyltransferase 2 isoform X1 [Cucumis sativus][more]
KAA0061573.16.6e-25083.24tRNA (guanine(37)-N1)-methyltransferase 2 [Cucumis melo var. makuwa][more]
XP_008458074.17.3e-24982.50PREDICTED: tRNA (guanine(37)-N1)-methyltransferase 2 [Cucumis melo][more]
Match NameE-valueIdentityDescription
F6HH452.5e-19163.17tRNA (guanine(37)-N1)-methyltransferase 2 OS=Vitis vinifera OX=29760 GN=VIT_11s0... [more]
Q93YU67.5e-17258.40tRNA (guanine(37)-N1)-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=At3... [more]
C5XX792.3e-15253.66tRNA (guanine(37)-N1)-methyltransferase OS=Sorghum bicolor OX=4558 GN=Sb04g02539... [more]
A9T6G54.5e-10842.91tRNA (guanine(37)-N1)-methyltransferase OS=Physcomitrium patens OX=3218 GN=PHYPA... [more]
F4NUJ63.3e-7132.26tRNA (guanine(37)-N1)-methyltransferase OS=Batrachochytrium dendrobatidis (strai... [more]
Match NameE-valueIdentityDescription
A0A0A0LFW83.2e-25884.54tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_2G0053... [more]
A0A5A7V0B13.2e-25083.24tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3C7L33.5e-24982.50tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103497610 ... [more]
A0A6J1DUW21.2e-24180.37tRNA (guanine(37)-N1)-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A5D3CFY16.5e-23578.77tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 G... [more]
Match NameE-valueIdentityDescription
AT3G56120.15.3e-17358.40S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT4G27340.14.4e-5029.34Met-10+ like family protein [more]
AT4G04670.12.0e-0727.84Met-10+ like family protein / kelch repeat-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.300.110coord: 166..225
e-value: 4.6E-17
score: 63.8
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 228..524
e-value: 9.7E-58
score: 196.8
NoneNo IPR availablePANTHERPTHR23245:SF36TRNA (GUANINE(37)-N1)-METHYLTRANSFERASEcoord: 2..529
NoneNo IPR availablePANTHERPTHR23245TRNA METHYLTRANSFERASEcoord: 2..529
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 268..325
e-value: 4.46809E-4
score: 37.7947
IPR030382SAM-dependent methyltransferase TRM5/TYW2-typePFAMPF02475Met_10coord: 170..476
e-value: 1.4E-62
score: 211.0
IPR030382SAM-dependent methyltransferase TRM5/TYW2-typePROSITEPS51684SAM_MT_TRM5_TYW2coord: 165..526
score: 100.271896
IPR025792tRNA (guanine(37)-N(1))-methyltransferase, eukaryoticHAMAPMF_03152TRM5coord: 2..526
score: 24.959179
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 170..526

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla97C11G209100.2Cla97C11G209100.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030488 tRNA methylation
cellular_component GO:0016020 membrane
cellular_component GO:0005759 mitochondrial matrix
cellular_component GO:0005634 nucleus
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function GO:0052906 tRNA (guanine(37)-N(1))-methyltransferase activity
molecular_function GO:0009019 tRNA (guanine-N1-)-methyltransferase activity