Carg01629 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg01629
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptiontRNA pseudouridine(38/39) synthase isoform X1
LocationCarg_Chr15: 2859493 .. 2860026 (-)
RNA-Seq ExpressionCarg01629
SyntenyCarg01629
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACGGAGCGGAACATTCCCTCGATTACAGAGGAGGTACGGGAGCAATGGAAACGACCTCAGGAACTGACGAGTTTCAACCCTTCGATGGAAACCTAATTGGAAATCTTCAATCCCAGCTTCGTCTTCTCCAAGGCAGGGTTAAGGTTTGAATGTCTACCCTCATTTCAACTTCCGGTTGATTAATGACATAATGGAGAAGAATCGGTAAAGGCATGACTGATGTTCGATCTCAATCCATTAATCTGTCAGGAATTAGAGGAGGAAAATTCGAATCTATCCTCTCGACTTGCAAGTTGCTGCTGTTCTGAGGTTTGGTCGACTATGTTAGAATGAAATCAGAGCTATTTGGTTGTCATGCTAGCCCAGCTAACCTTAGGGTAGATTTTTCGTCAGGGCTAACTAAACATGAATTCGTGCAGAGGAGGATGAACTTTGACGATGGGAGTAGTTCGATTGAGAAAAGCAAGAAATATGGAAGTCTGAAAGACAAGACTACTAAGAAGAAAGCAGTAGAAAAAACGCCAGGCTAG

mRNA sequence

ATGGACGGAGCGGAACATTCCCTCGATTACAGAGGAGGTACGGGAGCAATGGAAACGACCTCAGGAACTGACGAGTTTCAACCCTTCGATGGAAACCTAATTGGAAATCTTCAATCCCAGCTTCGTCTTCTCCAAGGCAGGGTTAAGGAATTAGAGGAGGAAAATTCGAATCTATCCTCTCGACTTGCAAGTTGCTGCTGTTCTGAGAGGAGGATGAACTTTGACGATGGGAGTAGTTCGATTGAGAAAAGCAAGAAATATGGAAGTCTGAAAGACAAGACTACTAAGAAGAAAGCAGTAGAAAAAACGCCAGGCTAG

Coding sequence (CDS)

ATGGACGGAGCGGAACATTCCCTCGATTACAGAGGAGGTACGGGAGCAATGGAAACGACCTCAGGAACTGACGAGTTTCAACCCTTCGATGGAAACCTAATTGGAAATCTTCAATCCCAGCTTCGTCTTCTCCAAGGCAGGGTTAAGGAATTAGAGGAGGAAAATTCGAATCTATCCTCTCGACTTGCAAGTTGCTGCTGTTCTGAGAGGAGGATGAACTTTGACGATGGGAGTAGTTCGATTGAGAAAAGCAAGAAATATGGAAGTCTGAAAGACAAGACTACTAAGAAGAAAGCAGTAGAAAAAACGCCAGGCTAG

Protein sequence

MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSQLRLLQGRVKELEEENSNLSSRLASCCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKKKAVEKTPG
Homology
BLAST of Carg01629 vs. NCBI nr
Match: KAG6578858.1 (putative F-actin-capping protein subunit beta, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 205.7 bits (522), Expect = 2.0e-49
Identity = 105/105 (100.00%), Postives = 105/105 (100.00%), Query Frame = 0

Query: 1   MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSQLRLLQGRVKELEEENSNLSS 60
           MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSQLRLLQGRVKELEEENSNLSS
Sbjct: 20  MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSQLRLLQGRVKELEEENSNLSS 79

Query: 61  RLASCCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKKKAVEKTPG 106
           RLASCCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKKKAVEKTPG
Sbjct: 80  RLASCCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKKKAVEKTPG 124

BLAST of Carg01629 vs. NCBI nr
Match: KAG7016392.1 (hypothetical protein SDJN02_21501, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 205.7 bits (522), Expect = 2.0e-49
Identity = 105/105 (100.00%), Postives = 105/105 (100.00%), Query Frame = 0

Query: 1   MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSQLRLLQGRVKELEEENSNLSS 60
           MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSQLRLLQGRVKELEEENSNLSS
Sbjct: 1   MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSQLRLLQGRVKELEEENSNLSS 60

Query: 61  RLASCCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKKKAVEKTPG 106
           RLASCCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKKKAVEKTPG
Sbjct: 61  RLASCCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKKKAVEKTPG 105

BLAST of Carg01629 vs. NCBI nr
Match: XP_023551060.1 (tRNA pseudouridine(38/39) synthase isoform X1 [Cucurbita pepo subsp. pepo] >XP_023551061.1 tRNA pseudouridine(38/39) synthase isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 203.4 bits (516), Expect = 9.8e-49
Identity = 103/105 (98.10%), Postives = 105/105 (100.00%), Query Frame = 0

Query: 1   MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSQLRLLQGRVKELEEENSNLSS 60
           MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSQLRLLQGRVKELEEENSNLS+
Sbjct: 1   MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSQLRLLQGRVKELEEENSNLST 60

Query: 61  RLASCCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKKKAVEKTPG 106
           RLA+CCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKKKAVEKTPG
Sbjct: 61  RLATCCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKKKAVEKTPG 105

BLAST of Carg01629 vs. NCBI nr
Match: XP_023551064.1 (tRNA pseudouridine(38/39) synthase isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 203.4 bits (516), Expect = 9.8e-49
Identity = 103/105 (98.10%), Postives = 105/105 (100.00%), Query Frame = 0

Query: 1   MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSQLRLLQGRVKELEEENSNLSS 60
           MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSQLRLLQGRVKELEEENSNLS+
Sbjct: 1   MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSQLRLLQGRVKELEEENSNLST 60

Query: 61  RLASCCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKKKAVEKTPG 106
           RLA+CCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKKKAVEKTPG
Sbjct: 61  RLATCCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKKKAVEKTPG 105

BLAST of Carg01629 vs. NCBI nr
Match: XP_022938732.1 (tRNA pseudouridine(38/39) synthase isoform X1 [Cucurbita moschata] >XP_022938734.1 tRNA pseudouridine(38/39) synthase isoform X1 [Cucurbita moschata])

HSP 1 Score: 199.1 bits (505), Expect = 1.9e-47
Identity = 101/105 (96.19%), Postives = 104/105 (99.05%), Query Frame = 0

Query: 1   MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSQLRLLQGRVKELEEENSNLSS 60
           MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQS+LRLLQGRVK LEEENSNLSS
Sbjct: 1   MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSRLRLLQGRVKVLEEENSNLSS 60

Query: 61  RLASCCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKKKAVEKTPG 106
           RLA+CCCSERRMNFDDGSSSIEKS+KYGSLKDKTTKKKAVEKTPG
Sbjct: 61  RLATCCCSERRMNFDDGSSSIEKSRKYGSLKDKTTKKKAVEKTPG 105

BLAST of Carg01629 vs. ExPASy TrEMBL
Match: A0A6J1FEX0 (tRNA pseudouridine(38/39) synthase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111444871 PE=3 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 9.0e-48
Identity = 101/105 (96.19%), Postives = 104/105 (99.05%), Query Frame = 0

Query: 1   MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSQLRLLQGRVKELEEENSNLSS 60
           MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQS+LRLLQGRVK LEEENSNLSS
Sbjct: 1   MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSRLRLLQGRVKVLEEENSNLSS 60

Query: 61  RLASCCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKKKAVEKTPG 106
           RLA+CCCSERRMNFDDGSSSIEKS+KYGSLKDKTTKKKAVEKTPG
Sbjct: 61  RLATCCCSERRMNFDDGSSSIEKSRKYGSLKDKTTKKKAVEKTPG 105

BLAST of Carg01629 vs. ExPASy TrEMBL
Match: A0A6J1FEX6 (tRNA pseudouridine(38/39) synthase isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111444871 PE=3 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 9.0e-48
Identity = 101/105 (96.19%), Postives = 104/105 (99.05%), Query Frame = 0

Query: 1   MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSQLRLLQGRVKELEEENSNLSS 60
           MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQS+LRLLQGRVK LEEENSNLSS
Sbjct: 1   MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSRLRLLQGRVKVLEEENSNLSS 60

Query: 61  RLASCCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKKKAVEKTPG 106
           RLA+CCCSERRMNFDDGSSSIEKS+KYGSLKDKTTKKKAVEKTPG
Sbjct: 61  RLATCCCSERRMNFDDGSSSIEKSRKYGSLKDKTTKKKAVEKTPG 105

BLAST of Carg01629 vs. ExPASy TrEMBL
Match: A0A6J1JWD4 (tRNA pseudouridine(38/39) synthase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111488950 PE=3 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 7.1e-45
Identity = 99/105 (94.29%), Postives = 101/105 (96.19%), Query Frame = 0

Query: 1   MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSQLRLLQGRVKELEEENSNLSS 60
           MDGAEHSLD+RG TGAMETTSGTDEFQPFD NLI NLQSQLRLLQGRVKELEEENSNLSS
Sbjct: 1   MDGAEHSLDHRGATGAMETTSGTDEFQPFDRNLIENLQSQLRLLQGRVKELEEENSNLSS 60

Query: 61  RLASCCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKKKAVEKTPG 106
           RLASC CSERRMNFDDGSSSIEKSKKYGSLKDKTT+KKAVEKTPG
Sbjct: 61  RLASCYCSERRMNFDDGSSSIEKSKKYGSLKDKTTEKKAVEKTPG 105

BLAST of Carg01629 vs. ExPASy TrEMBL
Match: A0A6J1JU85 (tRNA pseudouridine(38/39) synthase isoform X4 OS=Cucurbita maxima OX=3661 GN=LOC111488950 PE=3 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 7.1e-45
Identity = 99/105 (94.29%), Postives = 101/105 (96.19%), Query Frame = 0

Query: 1   MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSQLRLLQGRVKELEEENSNLSS 60
           MDGAEHSLD+RG TGAMETTSGTDEFQPFD NLI NLQSQLRLLQGRVKELEEENSNLSS
Sbjct: 1   MDGAEHSLDHRGATGAMETTSGTDEFQPFDRNLIENLQSQLRLLQGRVKELEEENSNLSS 60

Query: 61  RLASCCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKKKAVEKTPG 106
           RLASC CSERRMNFDDGSSSIEKSKKYGSLKDKTT+KKAVEKTPG
Sbjct: 61  RLASCYCSERRMNFDDGSSSIEKSKKYGSLKDKTTEKKAVEKTPG 105

BLAST of Carg01629 vs. ExPASy TrEMBL
Match: A0A6J1JQK1 (tRNA pseudouridine(38/39) synthase isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111488950 PE=3 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 4.6e-44
Identity = 98/105 (93.33%), Postives = 100/105 (95.24%), Query Frame = 0

Query: 1   MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSQLRLLQGRVKELEEENSNLSS 60
           MDGAEHSLD+RG TGAMETTSGTDEFQPFD NLI NLQSQLRLLQGRVK LEEENSNLSS
Sbjct: 1   MDGAEHSLDHRGATGAMETTSGTDEFQPFDRNLIENLQSQLRLLQGRVKVLEEENSNLSS 60

Query: 61  RLASCCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKKKAVEKTPG 106
           RLASC CSERRMNFDDGSSSIEKSKKYGSLKDKTT+KKAVEKTPG
Sbjct: 61  RLASCYCSERRMNFDDGSSSIEKSKKYGSLKDKTTEKKAVEKTPG 105

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6578858.12.0e-49100.00putative F-actin-capping protein subunit beta, partial [Cucurbita argyrosperma s... [more]
KAG7016392.12.0e-49100.00hypothetical protein SDJN02_21501, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_023551060.19.8e-4998.10tRNA pseudouridine(38/39) synthase isoform X1 [Cucurbita pepo subsp. pepo] >XP_0... [more]
XP_023551064.19.8e-4998.10tRNA pseudouridine(38/39) synthase isoform X3 [Cucurbita pepo subsp. pepo][more]
XP_022938732.11.9e-4796.19tRNA pseudouridine(38/39) synthase isoform X1 [Cucurbita moschata] >XP_022938734... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1FEX09.0e-4896.19tRNA pseudouridine(38/39) synthase isoform X1 OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1FEX69.0e-4896.19tRNA pseudouridine(38/39) synthase isoform X3 OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1JWD47.1e-4594.29tRNA pseudouridine(38/39) synthase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1JU857.1e-4594.29tRNA pseudouridine(38/39) synthase isoform X4 OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1JQK14.6e-4493.33tRNA pseudouridine(38/39) synthase isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 34..61
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 71..105
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..31
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 14..31
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 75..105

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg01629-RACarg01629-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001522 pseudouridine synthesis
biological_process GO:0008033 tRNA processing
molecular_function GO:0009982 pseudouridine synthase activity
molecular_function GO:0003723 RNA binding