Carg01425 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg01425
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	N-alpha-acetyltransferase MAK3-like
Location	Carg_Chr15: 4075738 .. 4077805 (-)
RNA-Seq Expression	Carg01425
Synteny	Carg01425

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAGTTGAAATTAATCAGCATTTTTATTTTTTTAGGGAACCAGGGCTTCAATACTTGGAAGGCATTCAAGTTCTCTCTTACCGATTAATACTCCAAAATTCGGCCGGATTCCTTCAGGCGACGTAGCAGTCTCCGGCGAGAAGATCAGAGAATGGAAGCGAAATTGGGCGGTGTGGTAGAAGGAGGAGAAACGAGGAAAGTTGAATTTGATGCATCGGAGATTGAGTACGTAAGCTATGGCGGTGAGCATCACTTACCTCTGATCATGAATCTGGTTGATCAAGAACTCAGCGAACCCTACTCCATATTCACTTATCGTTATTTCGTATACCTCTGGCCCCAACTTTCATTCCTGGTATTAATCTCGCTTTTGTATCAAATCTCTGACCAATTAGATCGTGTTTGTCGCGATTCCCTTTAATTTCTGTTCTATTCATTCTATTTTTGTTGTTTCCCCAGGCCTTCCATAGGGGAAAATGTGTCGGGACGGTGGTTTGTAAGATGGGAGAGCATCGGGGGGCTTTCAGAGGGTACATTGCCATGTTGGTTGTCATCAAGCCCTATAGAGGAAAAGGCATCGGTAAATTTTTCTTCTCTGGTTTCTGGGAAGTGGTTGTTGATAAATGAATTCTATGGTTTAGGTTTTCCTTGTAAGATTGATTTGTCATTCTGCCCATTTATTTGTTTTCGTTGCAAGGTTTATTTGTATGACTTACTTGGATTTAGATGATATTAGAATATGATATCATTGTTAGAATAATTTCTGTTTAACTGTGAGATACCACATCGATTAGAGAGAGGTAACGAGTGCATTTTTTATAAGAGTGTGGAAACCTCTCCCTAACAAACGCGTTTTAAGAACATTGAAGGAAAGATTGAAAGGGAAAGCCTAAAGAGGACAATATCTGCTAGTGGTGGGCTTAAGCCATTAGAAATGGTATCAGAGCCAGACACTAGGCGGTGTCCCAGCAAGGACGTTGGGCCCCTAAGGGGGGGTGGATTGTGAGATCCCACATCAATTGGAGAGAGGAACGAGTGTATTCGGAAACCTCTTCCTAGCAGACGCATTTTAAGAACCCTAAAGGACAGTCTAAAAGGACAATATCTCCTAGCGGCAGGCTTGGGCCGTTACATCAACGATCTTTGTCAAGAATGGCTGATACTCTTGGGTGTGATCCCAATTTTCTCCTTCTTTAGTTGACTGGAATATGATTTATTTGTATGCATAAAATGTACGGTACTTCATAATGTTTTCTATCTTAAATGGGTTATACACCCTTTTTTGTCACAAACCAATTAGGGTTCATAGATCTTCATTATCATAATTATTTCTCTTGATATTGTAGCTACCGAGCTCGTCACGAGGTCTATAAAAGCCATGATGGAATCAGGCTGTGATGAGGTATATTGACATCAATTATGGTATTTCCTCTGTCTTGCGTGTTCCAATATTTATGTCCTCTATTAATTCGTTAAAGGAGTCAAAGCAAATAACTTGTGCGTTCTCTTCTCTATGTTATATAGTTTGGTTTGTTACATTCTTACTCAAATCACTGAACTATTAGTTAATAGTGGATGGCTGTATAGCCTGTACCATCAAGTAATTTGACCTTTTACAAAAGAAAAGAAGCTCGAGCACGTGTTGCATCTGCAATATTGTACTTGTTATGTTATCTCCTTTAGATCCTAAGTGGTATTTTCAAGACAAGATCTCTTTCAGTCCCCCATGCCTATTTGGCATTGCTTAATAAGTTGGATTTGGAGACCTCCGCTTTCACCAAGCTCTGTTTCAATACAAGCCAATGCCGGGAATTATCTAAAGTAGTGATGAATTTATTAAAACGAACCCGAAGGTCTGGTAGAATAGGGGTAAAAGACCTTGAAACTCCTTGGAGCTAGCTACTTCGAATTATCGAGTATTTCTTTGAAAGTTAAAATGATATGATCTTGATTTCTGTGCACTGAATCAAATACAAAGTTTTCGCATATGGGAGAACGATCATATACAGATAAGGTAAAACATGCTACAAATATAGGGTTTATCGGTTTTCATTTCCTCTTCTAATCC

mRNA sequence

AAAGTTGAAATTAATCAGCATTTTTATTTTTTTAGGGAACCAGGGCTTCAATACTTGGAAGGCATTCAAGTTCTCTCTTACCGATTAATACTCCAAAATTCGGCCGGATTCCTTCAGGCGACGTAGCAGTCTCCGGCGAGAAGATCAGAGAATGGAAGCGAAATTGGGCGGTGTGGTAGAAGGAGGAGAAACGAGGAAAGTTGAATTTGATGCATCGGAGATTGAGTACGTAAGCTATGGCGGTGAGCATCACTTACCTCTGATCATGAATCTGGTTGATCAAGAACTCAGCGAACCCTACTCCATATTCACTTATCGTTATTTCGTATACCTCTGGCCCCAACTTTCATTCCTGGCCTTCCATAGGGGAAAATGTGTCGGGACGGTGGTTTGTAAGATGGGAGAGCATCGGGGGGCTTTCAGAGGGTACATTGCCATGTTGGTTGTCATCAAGCCCTATAGAGGAAAAGGCATCGCTACCGAGCTCGTCACGAGGTCTATAAAAGCCATGATGGAATCAGGCTGTGATGAGGTATATTGACATCAATTATGGTATTTCCTCTGTCTTGCGTGTTCCAATATTTATGTCCTCTATTAATTCGTTAAAGGAGTCAAAGCAAATAACTTGTGCGTTCTCTTCTCTATGTTATATAGTTTGGTTTGTTACATTCTTACTCAAATCACTGAACTATTAGTTAATAGTGGATGGCTGTATAGCCTGTACCATCAAGTAATTTGACCTTTTACAAAAGAAAAGAAGCTCGAGCACGTGTTGCATCTGCAATATTGTACTTGTTATGTTATCTCCTTTAGATCCTAAGTGGTATTTTCAAGACAAGATCTCTTTCAGTCCCCCATGCCTATTTGGCATTGCTTAATAAGTTGGATTTGGAGACCTCCGCTTTCACCAAGCTCTGTTTCAATACAAGCCAATGCCGGGAATTATCTAAAGTAGTGATGAATTTATTAAAACGAACCCGAAGGTCTGGTAGAATAGGGGTAAAAGACCTTGAAACTCCTTGGAGCTAGCTACTTCGAATTATCGAGTATTTCTTTGAAAGTTAAAATGATATGATCTTGATTTCTGTGCACTGAATCAAATACAAAGTTTTCGCATATGGGAGAACGATCATATACAGATAAGGTAAAACATGCTACAAATATAGGGTTTATCGGTTTTCATTTCCTCTTCTAATCC

Coding sequence (CDS)

ATGGAAGCGAAATTGGGCGGTGTGGTAGAAGGAGGAGAAACGAGGAAAGTTGAATTTGATGCATCGGAGATTGAGTACGTAAGCTATGGCGGTGAGCATCACTTACCTCTGATCATGAATCTGGTTGATCAAGAACTCAGCGAACCCTACTCCATATTCACTTATCGTTATTTCGTATACCTCTGGCCCCAACTTTCATTCCTGGCCTTCCATAGGGGAAAATGTGTCGGGACGGTGGTTTGTAAGATGGGAGAGCATCGGGGGGCTTTCAGAGGGTACATTGCCATGTTGGTTGTCATCAAGCCCTATAGAGGAAAAGGCATCGCTACCGAGCTCGTCACGAGGTCTATAAAAGCCATGATGGAATCAGGCTGTGATGAGGTATATTGA

Protein sequence

MEAKLGGVVEGGETRKVEFDASEIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVYLWPQLSFLAFHRGKCVGTVVCKMGEHRGAFRGYIAMLVVIKPYRGKGIATELVTRSIKAMMESGCDEVY

Homology

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BLAST of Carg01425 vs. NCBI nr
Match: KAG7016593.1 (N-alpha-acetyltransferase MAK3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 266.9 bits (681), Expect = 8.9e-68
Identity = 129/129 (100.00%), Postives = 129/129 (100.00%), Query Frame = 0


Query: 1   MEAKLGGVVEGGETRKVEFDASEIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVY 60
           MEAKLGGVVEGGETRKVEFDASEIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVY
Sbjct: 1   MEAKLGGVVEGGETRKVEFDASEIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVY 60

Query: 61  LWPQLSFLAFHRGKCVGTVVCKMGEHRGAFRGYIAMLVVIKPYRGKGIATELVTRSIKAM 120
           LWPQLSFLAFHRGKCVGTVVCKMGEHRGAFRGYIAMLVVIKPYRGKGIATELVTRSIKAM
Sbjct: 61  LWPQLSFLAFHRGKCVGTVVCKMGEHRGAFRGYIAMLVVIKPYRGKGIATELVTRSIKAM 120

Query: 121 MESGCDEVY 130
           MESGCDEVY
Sbjct: 121 MESGCDEVY 129

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BLAST of Carg01425 vs. NCBI nr
Match: KAG6579067.1 (N-alpha-acetyltransferase MAK3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 263.8 bits (673), Expect = 7.5e-67
Identity = 128/128 (100.00%), Postives = 128/128 (100.00%), Query Frame = 0


Query: 1   MEAKLGGVVEGGETRKVEFDASEIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVY 60
           MEAKLGGVVEGGETRKVEFDASEIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVY
Sbjct: 1   MEAKLGGVVEGGETRKVEFDASEIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVY 60

Query: 61  LWPQLSFLAFHRGKCVGTVVCKMGEHRGAFRGYIAMLVVIKPYRGKGIATELVTRSIKAM 120
           LWPQLSFLAFHRGKCVGTVVCKMGEHRGAFRGYIAMLVVIKPYRGKGIATELVTRSIKAM
Sbjct: 61  LWPQLSFLAFHRGKCVGTVVCKMGEHRGAFRGYIAMLVVIKPYRGKGIATELVTRSIKAM 120

Query: 121 MESGCDEV 129
           MESGCDEV
Sbjct: 121 MESGCDEV 128

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BLAST of Carg01425 vs. NCBI nr
Match: XP_022939222.1 (N-alpha-acetyltransferase MAK3-like [Cucurbita moschata])

HSP 1 Score: 263.8 bits (673), Expect = 7.5e-67
Identity = 128/128 (100.00%), Postives = 128/128 (100.00%), Query Frame = 0


Query: 1   MEAKLGGVVEGGETRKVEFDASEIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVY 60
           MEAKLGGVVEGGETRKVEFDASEIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVY
Sbjct: 1   MEAKLGGVVEGGETRKVEFDASEIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVY 60

Query: 61  LWPQLSFLAFHRGKCVGTVVCKMGEHRGAFRGYIAMLVVIKPYRGKGIATELVTRSIKAM 120
           LWPQLSFLAFHRGKCVGTVVCKMGEHRGAFRGYIAMLVVIKPYRGKGIATELVTRSIKAM
Sbjct: 61  LWPQLSFLAFHRGKCVGTVVCKMGEHRGAFRGYIAMLVVIKPYRGKGIATELVTRSIKAM 120

Query: 121 MESGCDEV 129
           MESGCDEV
Sbjct: 121 MESGCDEV 128

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BLAST of Carg01425 vs. NCBI nr
Match: XP_023549721.1 (N-alpha-acetyltransferase MAK3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 261.2 bits (666), Expect = 4.9e-66
Identity = 127/128 (99.22%), Postives = 127/128 (99.22%), Query Frame = 0


Query: 1   MEAKLGGVVEGGETRKVEFDASEIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVY 60
           MEAKL GVVEGGETRKVEFDASEIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVY
Sbjct: 1   MEAKLDGVVEGGETRKVEFDASEIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVY 60

Query: 61  LWPQLSFLAFHRGKCVGTVVCKMGEHRGAFRGYIAMLVVIKPYRGKGIATELVTRSIKAM 120
           LWPQLSFLAFHRGKCVGTVVCKMGEHRGAFRGYIAMLVVIKPYRGKGIATELVTRSIKAM
Sbjct: 61  LWPQLSFLAFHRGKCVGTVVCKMGEHRGAFRGYIAMLVVIKPYRGKGIATELVTRSIKAM 120

Query: 121 MESGCDEV 129
           MESGCDEV
Sbjct: 121 MESGCDEV 128

[Close]

BLAST of Carg01425 vs. NCBI nr
Match: XP_022992874.1 (N-alpha-acetyltransferase MAK3-like [Cucurbita maxima])

HSP 1 Score: 254.2 bits (648), Expect = 6.0e-64
Identity = 124/128 (96.88%), Postives = 124/128 (96.88%), Query Frame = 0


Query: 1   MEAKLGGVVEGGETRKVEFDASEIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVY 60
           MEAKL  VVEGGET KVEFDA EIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVY
Sbjct: 1   MEAKLDSVVEGGETMKVEFDALEIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVY 60

Query: 61  LWPQLSFLAFHRGKCVGTVVCKMGEHRGAFRGYIAMLVVIKPYRGKGIATELVTRSIKAM 120
           LWPQLSFLAFHRGKCVGTVVCKMGEHRGAFRGYIAMLVVIKPYRGKGIATELVTRSIKAM
Sbjct: 61  LWPQLSFLAFHRGKCVGTVVCKMGEHRGAFRGYIAMLVVIKPYRGKGIATELVTRSIKAM 120

Query: 121 MESGCDEV 129
           MESGCDEV
Sbjct: 121 MESGCDEV 128

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BLAST of Carg01425 vs. ExPASy Swiss-Prot
Match: O80438 (N-alpha-acetyltransferase MAK3 OS=Arabidopsis thaliana OX=3702 GN=MAK3 PE=1 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 2.9e-53
Identity = 97/113 (85.84%), Postives = 105/113 (92.92%), Query Frame = 0


Query: 16  KVEFDASEIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVYLWPQLSFLAFHRGKC 75
           K EFD  EIEY SY GEHHLPLIM+LVDQELSEPYSIFTYRYFVYLWPQL FLAFH+GKC
Sbjct: 8   KEEFDEGEIEYTSYAGEHHLPLIMSLVDQELSEPYSIFTYRYFVYLWPQLCFLAFHKGKC 67

Query: 76  VGTVVCKMGEHRGAFRGYIAMLVVIKPYRGKGIATELVTRSIKAMMESGCDEV 129
           VGT+VCKMG+HR  FRGYIAMLVVIKPYRG+GIA+ELVTR+IKAMMESGC+EV
Sbjct: 68  VGTIVCKMGDHRQTFRGYIAMLVVIKPYRGRGIASELVTRAIKAMMESGCEEV 120

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BLAST of Carg01425 vs. ExPASy Swiss-Prot
Match: Q147X3 (N-alpha-acetyltransferase 30 OS=Homo sapiens OX=9606 GN=NAA30 PE=1 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 1.3e-26
Identity = 65/124 (52.42%), Postives = 79/124 (63.71%), Query Frame = 0


Query: 6   GGVVEGGETRKVEFDASEIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVYLWPQL 65
           G  VE GE R        I YV Y  E  +P IM L+ ++LSEPYSI+TYRYF++ WPQL
Sbjct: 202 GREVEPGEDR-------TIRYVRYESELQMPDIMRLITKDLSEPYSIYTYRYFIHNWPQL 261

Query: 66  SFLAFHRGKCVGTVVCKMGEHRGAF-RGYIAMLVVIKPYRGKGIATELVTRSIKAMMESG 125
            FLA    +CVG +VCK+  H+  F RGYIAML V   YR  GI T LV ++I AM+E  
Sbjct: 262 CFLAMVGEECVGAIVCKLDMHKKMFRRGYIAMLAVDSKYRRNGIGTNLVKKAIYAMVEGD 318

Query: 126 CDEV 129
           CDEV
Sbjct: 322 CDEV 318

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BLAST of Carg01425 vs. ExPASy Swiss-Prot
Match: Q8CES0 (N-alpha-acetyltransferase 30 OS=Mus musculus OX=10090 GN=Naa30 PE=1 SV=2)

HSP 1 Score: 119.8 bits (299), Expect = 2.3e-26
Identity = 64/129 (49.61%), Postives = 80/129 (62.02%), Query Frame = 0


Query: 1   MEAKLGGVVEGGETRKVEFDASEIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVY 60
           + +  G   E GE R        I YV Y  E  +P IM L+ ++LSEPYSI+TYRYF++
Sbjct: 199 LRSSQGREAEPGEDR-------TIRYVRYESELQMPDIMRLITKDLSEPYSIYTYRYFIH 258

Query: 61  LWPQLSFLAFHRGKCVGTVVCKMGEHRGAF-RGYIAMLVVIKPYRGKGIATELVTRSIKA 120
            WPQL FLA    +CVG +VCK+  H+  F RGYIAML V   YR  GI T LV ++I A
Sbjct: 259 NWPQLCFLAMVGEECVGAIVCKLDMHKKMFRRGYIAMLAVDSKYRRNGIGTNLVKKAIYA 318

Query: 121 MMESGCDEV 129
           M+E  CDEV
Sbjct: 319 MVEGDCDEV 320

[Close]

BLAST of Carg01425 vs. ExPASy Swiss-Prot
Match: Q95RC0 (N-alpha-acetyltransferase 30A OS=Drosophila melanogaster OX=7227 GN=Naa30A PE=2 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 3.0e-26
Identity = 59/108 (54.63%), Postives = 71/108 (65.74%), Query Frame = 0


Query: 21  ASEIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVYLWPQLSFLAFHRGKCVGTVV 80
           A EI Y  Y  EH +  IM L+  ELSEPYSI+TYRYF+Y WP+L FLA H  + VG +V
Sbjct: 226 ADEIVYKEYEAEHQMHDIMRLIQAELSEPYSIYTYRYFIYNWPKLCFLASHDNQYVGAIV 285

Query: 81  CKMGEHRGAFRGYIAMLVVIKPYRGKGIATELVTRSIKAMMESGCDEV 129
           CK+  H    RGYIAML V K YR   I T LVT++I+AM+    DEV
Sbjct: 286 CKLDMHMNVRRGYIAMLAVRKEYRKLKIGTTLVTKAIEAMLADNADEV 333

[Close]

BLAST of Carg01425 vs. ExPASy Swiss-Prot
Match: Q0IHH1 (N-alpha-acetyltransferase 30 OS=Xenopus laevis OX=8355 GN=naa30 PE=2 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 8.7e-26
Identity = 64/125 (51.20%), Postives = 79/125 (63.20%), Query Frame = 0


Query: 5   LGGVVEGGETRKVEFDASEIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVYLWPQ 64
           LG  V  GE      +   I YV Y  E  +  IM L+ ++LSEPYSI+TYRYF++ WPQ
Sbjct: 111 LGNHVSQGE------NDDTIRYVRYESELQMADIMRLITRDLSEPYSIYTYRYFIHNWPQ 170

Query: 65  LSFLAFHRGKCVGTVVCKMGEHRGAF-RGYIAMLVVIKPYRGKGIATELVTRSIKAMMES 124
           L FLA    +CVG +VCK+  H+  F RGYIAML V   YR KGI T LV ++I AM+E 
Sbjct: 171 LCFLAMVGEECVGAIVCKLDMHKKMFRRGYIAMLAVDSKYRRKGIGTHLVKKAIYAMVEG 229

Query: 125 GCDEV 129
            CDEV
Sbjct: 231 DCDEV 229

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BLAST of Carg01425 vs. ExPASy TrEMBL
Match: A0A6J1FG73 (N-alpha-acetyltransferase MAK3-like OS=Cucurbita moschata OX=3662 GN=LOC111445200 PE=3 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 3.6e-67
Identity = 128/128 (100.00%), Postives = 128/128 (100.00%), Query Frame = 0


Query: 1   MEAKLGGVVEGGETRKVEFDASEIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVY 60
           MEAKLGGVVEGGETRKVEFDASEIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVY
Sbjct: 1   MEAKLGGVVEGGETRKVEFDASEIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVY 60

Query: 61  LWPQLSFLAFHRGKCVGTVVCKMGEHRGAFRGYIAMLVVIKPYRGKGIATELVTRSIKAM 120
           LWPQLSFLAFHRGKCVGTVVCKMGEHRGAFRGYIAMLVVIKPYRGKGIATELVTRSIKAM
Sbjct: 61  LWPQLSFLAFHRGKCVGTVVCKMGEHRGAFRGYIAMLVVIKPYRGKGIATELVTRSIKAM 120

Query: 121 MESGCDEV 129
           MESGCDEV
Sbjct: 121 MESGCDEV 128

[Close]

BLAST of Carg01425 vs. ExPASy TrEMBL
Match: A0A6J1JYP2 (N-alpha-acetyltransferase MAK3-like OS=Cucurbita maxima OX=3661 GN=LOC111489077 PE=3 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 2.9e-64
Identity = 124/128 (96.88%), Postives = 124/128 (96.88%), Query Frame = 0


Query: 1   MEAKLGGVVEGGETRKVEFDASEIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVY 60
           MEAKL  VVEGGET KVEFDA EIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVY
Sbjct: 1   MEAKLDSVVEGGETMKVEFDALEIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVY 60

Query: 61  LWPQLSFLAFHRGKCVGTVVCKMGEHRGAFRGYIAMLVVIKPYRGKGIATELVTRSIKAM 120
           LWPQLSFLAFHRGKCVGTVVCKMGEHRGAFRGYIAMLVVIKPYRGKGIATELVTRSIKAM
Sbjct: 61  LWPQLSFLAFHRGKCVGTVVCKMGEHRGAFRGYIAMLVVIKPYRGKGIATELVTRSIKAM 120

Query: 121 MESGCDEV 129
           MESGCDEV
Sbjct: 121 MESGCDEV 128

[Close]

BLAST of Carg01425 vs. ExPASy TrEMBL
Match: A0A6J1GQN0 (N-alpha-acetyltransferase MAK3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456211 PE=3 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 2.4e-63
Identity = 121/128 (94.53%), Postives = 124/128 (96.88%), Query Frame = 0


Query: 1   MEAKLGGVVEGGETRKVEFDASEIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVY 60
           MEAK+ GVVE GETRKVEF+ASEIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVY
Sbjct: 1   MEAKVDGVVEEGETRKVEFEASEIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVY 60

Query: 61  LWPQLSFLAFHRGKCVGTVVCKMGEHRGAFRGYIAMLVVIKPYRGKGIATELVTRSIKAM 120
           LWPQLSFLAFHRGKCVGTVVCKMGEHRG FRGYIAMLVVIKPYRGKGIATELVTRSI+ M
Sbjct: 61  LWPQLSFLAFHRGKCVGTVVCKMGEHRGTFRGYIAMLVVIKPYRGKGIATELVTRSIRVM 120

Query: 121 MESGCDEV 129
           MESGCDEV
Sbjct: 121 MESGCDEV 128

[Close]

BLAST of Carg01425 vs. ExPASy TrEMBL
Match: A0A6J1JKL0 (N-alpha-acetyltransferase MAK3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111487752 PE=3 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 4.6e-62
Identity = 118/128 (92.19%), Postives = 123/128 (96.09%), Query Frame = 0


Query: 1   MEAKLGGVVEGGETRKVEFDASEIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVY 60
           MEA++ GV E GETRKVEF+ASEIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVY
Sbjct: 1   MEARVDGVAEEGETRKVEFEASEIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVY 60

Query: 61  LWPQLSFLAFHRGKCVGTVVCKMGEHRGAFRGYIAMLVVIKPYRGKGIATELVTRSIKAM 120
           LWPQLSFLAFHRGKCVGTVVCKMGEHRG FRGYIAMLVVIKPYRGKGIA+ELVTRSI+ M
Sbjct: 61  LWPQLSFLAFHRGKCVGTVVCKMGEHRGTFRGYIAMLVVIKPYRGKGIASELVTRSIRVM 120

Query: 121 MESGCDEV 129
           MESGCDEV
Sbjct: 121 MESGCDEV 128

[Close]

BLAST of Carg01425 vs. ExPASy TrEMBL
Match: A0A0A0KT08 (N-acetyltransferase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G311820 PE=3 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 6.0e-62
Identity = 119/128 (92.97%), Postives = 123/128 (96.09%), Query Frame = 0


Query: 1   MEAKLGGVVEGGETRKVEFDASEIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVY 60
           ME+KL GVVE  ETRKV+FDASEIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVY
Sbjct: 1   MESKLDGVVEEEETRKVQFDASEIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVY 60

Query: 61  LWPQLSFLAFHRGKCVGTVVCKMGEHRGAFRGYIAMLVVIKPYRGKGIATELVTRSIKAM 120
           LWPQLSFLAFHRGKCVGTVVCKMGEHRG FRGYIAMLVVIKPYRG+GIA+ELVTRSIK M
Sbjct: 61  LWPQLSFLAFHRGKCVGTVVCKMGEHRGTFRGYIAMLVVIKPYRGEGIASELVTRSIKVM 120

Query: 121 MESGCDEV 129
           MESGCDEV
Sbjct: 121 MESGCDEV 128

[Close]

BLAST of Carg01425 vs. TAIR 10
Match: AT2G38130.1 (Acyl-CoA N-acyltransferases (NAT) superfamily protein )

HSP 1 Score: 209.1 bits (531), Expect = 2.0e-54
Identity = 97/113 (85.84%), Postives = 105/113 (92.92%), Query Frame = 0


Query: 16  KVEFDASEIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVYLWPQLSFLAFHRGKC 75
           K EFD  EIEY SY GEHHLPLIM+LVDQELSEPYSIFTYRYFVYLWPQL FLAFH+GKC
Sbjct: 8   KEEFDEGEIEYTSYAGEHHLPLIMSLVDQELSEPYSIFTYRYFVYLWPQLCFLAFHKGKC 67

Query: 76  VGTVVCKMGEHRGAFRGYIAMLVVIKPYRGKGIATELVTRSIKAMMESGCDEV 129
           VGT+VCKMG+HR  FRGYIAMLVVIKPYRG+GIA+ELVTR+IKAMMESGC+EV
Sbjct: 68  VGTIVCKMGDHRQTFRGYIAMLVVIKPYRGRGIASELVTRAIKAMMESGCEEV 120

[Close]

BLAST of Carg01425 vs. TAIR 10
Match: AT2G38130.2 (Acyl-CoA N-acyltransferases (NAT) superfamily protein )

HSP 1 Score: 209.1 bits (531), Expect = 2.0e-54
Identity = 97/113 (85.84%), Postives = 105/113 (92.92%), Query Frame = 0


Query: 16  KVEFDASEIEYVSYGGEHHLPLIMNLVDQELSEPYSIFTYRYFVYLWPQLSFLAFHRGKC 75
           K EFD  EIEY SY GEHHLPLIM+LVDQELSEPYSIFTYRYFVYLWPQL FLAFH+GKC
Sbjct: 8   KEEFDEGEIEYTSYAGEHHLPLIMSLVDQELSEPYSIFTYRYFVYLWPQLCFLAFHKGKC 67

Query: 76  VGTVVCKMGEHRGAFRGYIAMLVVIKPYRGKGIATELVTRSIKAMMESGCDEV 129
           VGT+VCKMG+HR  FRGYIAMLVVIKPYRG+GIA+ELVTR+IKAMMESGC+EV
Sbjct: 68  VGTIVCKMGDHRQTFRGYIAMLVVIKPYRGRGIASELVTRAIKAMMESGCEEV 120

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BLAST of Carg01425 vs. TAIR 10
Match: AT5G13780.1 (Acyl-CoA N-acyltransferases (NAT) superfamily protein )

HSP 1 Score: 52.8 bits (125), Expect = 2.5e-07
Identity = 29/76 (38.16%), Postives = 44/76 (57.89%), Query Frame = 0


Query: 46  LSEPYSIFTYRYFVYLWPQLSFLA-FHRGKCVGTVVCKMGEHRGAFRGYIAMLVVIKPYR 105
           L E Y +  Y Y +  WPQL ++A  + G+ VG V+ KM E      G+I  L V++ +R
Sbjct: 23  LPENYQMKYYLYHILSWPQLLYVAEDYNGRIVGYVLAKMEEESNECHGHITSLAVLRTHR 82

Query: 106 GKGIATELVTRSIKAM 121
             G+AT+L+T +  AM
Sbjct: 83  KLGLATKLMTAAQAAM 98

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BLAST of Carg01425 vs. TAIR 10
Match: AT5G11340.1 (Acyl-CoA N-acyltransferases (NAT) superfamily protein )

HSP 1 Score: 44.7 bits (104), Expect = 6.7e-05
Identity = 22/63 (34.92%), Postives = 34/63 (53.97%), Query Frame = 0


Query: 68  LAFHRGKCVGTVVCKM-GEHRGAFRGYIAMLVVIKPYRGKGIATELVTRSIKAMMESGCD 127
           LA++   CVG + C++  +  GA R YI  L V+ PYRG GI + L+   +    +    
Sbjct: 51  LAYYNDICVGAIACRLEKKESGAMRVYIMTLGVLAPYRGIGIGSNLLNHVLDMCSKQNMC 110

Query: 128 EVY 130
           E+Y
Sbjct: 111 EIY 113

The following BLAST results are available for this feature:

BLAST of Carg01425 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7016593.1	8.9e-68	100.00	N-alpha-acetyltransferase MAK3 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6579067.1	7.5e-67	100.00	N-alpha-acetyltransferase MAK3, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022939222.1	7.5e-67	100.00	N-alpha-acetyltransferase MAK3-like [Cucurbita moschata]	[more]
XP_023549721.1	4.9e-66	99.22	N-alpha-acetyltransferase MAK3-like [Cucurbita pepo subsp. pepo]	[more]
XP_022992874.1	6.0e-64	96.88	N-alpha-acetyltransferase MAK3-like [Cucurbita maxima]	[more]

BLAST of Carg01425 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O80438	2.9e-53	85.84	N-alpha-acetyltransferase MAK3 OS=Arabidopsis thaliana OX=3702 GN=MAK3 PE=1 SV=1	[more]
Q147X3	1.3e-26	52.42	N-alpha-acetyltransferase 30 OS=Homo sapiens OX=9606 GN=NAA30 PE=1 SV=1	[more]
Q8CES0	2.3e-26	49.61	N-alpha-acetyltransferase 30 OS=Mus musculus OX=10090 GN=Naa30 PE=1 SV=2	[more]
Q95RC0	3.0e-26	54.63	N-alpha-acetyltransferase 30A OS=Drosophila melanogaster OX=7227 GN=Naa30A PE=2 ...	[more]
Q0IHH1	8.7e-26	51.20	N-alpha-acetyltransferase 30 OS=Xenopus laevis OX=8355 GN=naa30 PE=2 SV=1	[more]

BLAST of Carg01425 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FG73	3.6e-67	100.00	N-alpha-acetyltransferase MAK3-like OS=Cucurbita moschata OX=3662 GN=LOC11144520...	[more]
A0A6J1JYP2	2.9e-64	96.88	N-alpha-acetyltransferase MAK3-like OS=Cucurbita maxima OX=3661 GN=LOC111489077 ...	[more]
A0A6J1GQN0	2.4e-63	94.53	N-alpha-acetyltransferase MAK3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1JKL0	4.6e-62	92.19	N-alpha-acetyltransferase MAK3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A0A0KT08	6.0e-62	92.97	N-acetyltransferase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_...	[more]

BLAST of Carg01425 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38130.1	2.0e-54	85.84	Acyl-CoA N-acyltransferases (NAT) superfamily protein	[more]
AT2G38130.2	2.0e-54	85.84	Acyl-CoA N-acyltransferases (NAT) superfamily protein	[more]
AT5G13780.1	2.5e-07	38.16	Acyl-CoA N-acyltransferases (NAT) superfamily protein	[more]
AT5G11340.1	6.7e-05	34.92	Acyl-CoA N-acyltransferases (NAT) superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000182	GNAT domain	PFAM	PF00583	Acetyltransf_1	coord: 59..129 e-value: 5.1E-10 score: 39.6
IPR000182	GNAT domain	PROSITE	PS51186	GNAT	coord: 22..129 score: 15.421492
None	No IPR available	GENE3D	3.40.630.30		coord: 31..129 e-value: 7.8E-25 score: 89.6
None	No IPR available	CDD	cd04301	NAT_SF	coord: 66..129 e-value: 2.61052E-8 score: 45.7297
IPR044542	N-alpha-acetyltransferase 30-like	PANTHER	PTHR45896	N-ALPHA-ACETYLTRANSFERASE 30	coord: 13..128
IPR016181	Acyl-CoA N-acyltransferase	SUPERFAMILY	55729	Acyl-CoA N-acyltransferases (Nat)	coord: 32..129

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg01425-RA	Carg01425-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0017196	N-terminal peptidyl-methionine acetylation
cellular_component	GO:0031417	NatC complex
molecular_function	GO:0004596	peptide alpha-N-acetyltransferase activity
molecular_function	GO:0008080	N-acetyltransferase activity