CSPI06G28430 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI06G28430
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	PAMP-induced secreted peptide 2
Location	Chr6: 24827410 .. 24827860 (-)
RNA-Seq Expression	CSPI06G28430
Synteny	CSPI06G28430

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGGAGCATCACCAACATTTTCATCGGCTTCATTCTGGTGGCTGCGCTGTTGGGCAATGGCGGGTTAGTTATTGTAACAGACGCCAGGCCATTGACCCTCGTGGGCTTGGGCTCGGGCTCGGCGGCAACAATAGCTGAAGATTTCTTTGAAGGCCTTTCTTTGGGAGCCATTAAGCAGTCAGGTCCCAGCGCTGGTGGAGATGGTCACAAATTTGTTAATTACGACACGCTTGGAGGTATCAAGGATTCGGGCCCCGCACCTGGAGATGGACATAGACACATCACCTCCTCACATCATTGAAGATGGAAAGTCCAAATGTTCGTTCGTTAGTTCAGTCGCAACTCCAAGTAGATTAAATAACAATTTGAAAGGGTTGAAAGTTGTTGACGCTATCATATACTTTTATACTTTATTTATTTTGTTCTTTTTGTTTGATTTTGTATCATTA

mRNA sequence

ATGAGGAGCATCACCAACATTTTCATCGGCTTCATTCTGGTGGCTGCGCTGTTGGGCAATGGCGGGTTAGTTATTGTAACAGACGCCAGGCCATTGACCCTCGTGGGCTTGGGCTCGGGCTCGGCGGCAACAATAGCTGAAGATTTCTTTGAAGGCCTTTCTTTGGGAGCCATTAAGCAGTCAGGTCCCAGCGCTGGTGGAGATGGTCACAAATTTGTTAATTACGACACGCTTGGAGGTATCAAGGATTCGGGCCCCGCACCTGGAGATGGACATAGACACATCACCTCCTCACATCATTGAAGATGGAAAGTCCAAATGTTCGTTCGTTAGTTCAGTCGCAACTCCAAGTAGATTAAATAACAATTTGAAAGGGTTGAAAGTTGTTGACGCTATCATATACTTTTATACTTTATTTATTTTGTTCTTTTTGTTTGATTTTGTATCATTA

Coding sequence (CDS)

ATGAGGAGCATCACCAACATTTTCATCGGCTTCATTCTGGTGGCTGCGCTGTTGGGCAATGGCGGGTTAGTTATTGTAACAGACGCCAGGCCATTGACCCTCGTGGGCTTGGGCTCGGGCTCGGCGGCAACAATAGCTGAAGATTTCTTTGAAGGCCTTTCTTTGGGAGCCATTAAGCAGTCAGGTCCCAGCGCTGGTGGAGATGGTCACAAATTTGTTAATTACGACACGCTTGGAGGTATCAAGGATTCGGGCCCCGCACCTGGAGATGGACATAGACACATCACCTCCTCACATCATTGA

Protein sequence

MRSITNIFIGFILVAALLGNGGLVIVTDARPLTLVGLGSGSAATIAEDFFEGLSLGAIKQSGPSAGGDGHKFVNYDTLGGIKDSGPAPGDGHRHITSSHH*

Homology

[Close]

BLAST of CSPI06G28430 vs. ExPASy Swiss-Prot
Match: F4JRC5 (PAMP-induced secreted peptide 2 OS=Arabidopsis thaliana OX=3702 GN=PIP2 PE=1 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 8.7e-05
Identity = 36/83 (43.37%), Postives = 50/83 (60.24%), Query Frame = 0


Query: 12 ILVAALLGNGGLVIVTDARPLTLVGLGSGSAATIAEDFFEGLSLGAIKQSGPSAGGDGHK 71
          IL   L+G+    ++ ++RPL L        A++    F+GLSLG+IK SGPS  G+GHK
Sbjct: 11 ILFFMLIGS----VLVESRPLGLTKTEEKFVASL----FDGLSLGSIKDSGPSP-GEGHK 70

Query: 72 FVN-YDTLGGIKDSGPAP-GDGH 93
           V+  DT   +K SGP+P G GH
Sbjct: 71 VVDRKDTFRFVKHSGPSPSGPGH 84

[Close]

BLAST of CSPI06G28430 vs. ExPASy TrEMBL
Match: A0A0A0KIE2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G491710 PE=4 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 2.3e-45
Identity = 97/100 (97.00%), Postives = 98/100 (98.00%), Query Frame = 0


Query: 1   MRSITNIFIGFILVAALLGNGGLVIVTDARPLTLVGLGSGSAATIAEDFFEGLSLGAIKQ 60
           MRSITNIFIGFILVAALLGNGGLV VTDARPLTL+ LGSGSAATIAEDFFEGLSLGAIKQ
Sbjct: 1   MRSITNIFIGFILVAALLGNGGLVTVTDARPLTLMRLGSGSAATIAEDFFEGLSLGAIKQ 60

Query: 61  SGPSAGGDGHKFVNYDTLGGIKDSGPAPGDGHRHITSSHH 101
           SGPSAGGDGHKFVNYDTLGGIKDSGPAPGDGHRHITSSHH
Sbjct: 61  SGPSAGGDGHKFVNYDTLGGIKDSGPAPGDGHRHITSSHH 100

[Close]

BLAST of CSPI06G28430 vs. ExPASy TrEMBL
Match: A0A5D3CLN9 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004490 PE=4 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 2.9e-35
Identity = 80/97 (82.47%), Postives = 86/97 (88.66%), Query Frame = 0


Query: 4   ITNIFIGFILVAALLGNGGLVIVTDARPLTLVGLGSGSAATIAEDFFEGLSLGAIKQSGP 63
           ++ IFIG ILV ALL NGGLVIVT+ARPL L  +GSGSAA IAEDFFEGLSLGAIKQSGP
Sbjct: 1   MSKIFIGLILVVALLDNGGLVIVTEARPLGL--MGSGSAAAIAEDFFEGLSLGAIKQSGP 60

Query: 64  SAGGDGHKFVNYDTLGGIKDSGPAPGDGHRHITSSHH 101
           SAGGDGHKFVNYDT GG+KDSGP PGDGH H+TSSHH
Sbjct: 61  SAGGDGHKFVNYDTFGGMKDSGPTPGDGHSHVTSSHH 95

[Close]

BLAST of CSPI06G28430 vs. ExPASy TrEMBL
Match: A0A059AZU8 (Uncharacterized protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_H02229 PE=4 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 1.2e-12
Identity = 47/99 (47.47%), Postives = 65/99 (65.66%), Query Frame = 0


Query: 1   MRSITNIFIGFILVAALLGNGGLVIVTDARPLTLVGLGSGSAATIAEDFFEGLSLGAIKQ 60
           MRS T + +  +LV A+L    + +  +ARP  ++             FF+GLSLGAIKQ
Sbjct: 1   MRSATRV-LHILLVLAML--CAVFVANEARPFNVMET-KAPVGVACGGFFDGLSLGAIKQ 60

Query: 61  SGPSAGGDGHKFVNYDTLGGIKDSGPAPGDGHRHITSSH 100
           +GPS  G+GHKF N +TLGGIKDSGP+PG+GH H+T ++
Sbjct: 61  AGPSP-GEGHKFTNSETLGGIKDSGPSPGEGHGHVTGTN 94

[Close]

BLAST of CSPI06G28430 vs. ExPASy TrEMBL
Match: V7CCF8 (Uncharacterized protein OS=Phaseolus vulgaris OX=3885 GN=PHAVU_003G239800g PE=4 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 3.4e-12
Identity = 48/98 (48.98%), Postives = 63/98 (64.29%), Query Frame = 0


Query: 1  MRSITNIFIGFILVAALLGNGGLVIVTDARPLTLVGLGSGSAATIAEDFFEGLSLGAIKQ 60
          M SIT      +LV  ++  G  ++  +ARPL++ G G G       DFF+ LSLGAIK+
Sbjct: 1  MTSITKTRAFVVLVLLVMSTG--LMGLEARPLSISGTGEGVEVV---DFFDWLSLGAIKE 60

Query: 61 SGPSAGGDGHKFVNYDTLGGIKDSGPAP-GDGHRHITS 98
          SGPS GG GH+F N +TLGGIKDSGP+  G+GH+   S
Sbjct: 61 SGPSPGGKGHRFTNSNTLGGIKDSGPSSGGEGHKFTNS 93

[Close]

BLAST of CSPI06G28430 vs. ExPASy TrEMBL
Match: D7TTE3 (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_07s0129g01010 PE=4 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 7.6e-12
Identity = 43/93 (46.24%), Postives = 57/93 (61.29%), Query Frame = 0


Query: 8   FIGFILVAALLGNGGLVIVTDARPLTLVGLGSGSAATIAEDFFEGLSLGAIKQSGPSAGG 67
           F+ F+ +  +L +    I    RP  ++    G        FF+GLSLGAIKQSGPS  G
Sbjct: 7   FVSFLFLVLVLNS----IAIHGRPFNVLKKPRGPDGEEMRGFFDGLSLGAIKQSGPSP-G 66

Query: 68  DGHKFVNYDTLGGIKDSGPAPGDGHRHITSSHH 101
           +GHKF N  TLGGIKDSGP+PG+GH+++    H
Sbjct: 67  NGHKFTNAGTLGGIKDSGPSPGEGHKYVDGMPH 94

[Close]

BLAST of CSPI06G28430 vs. NCBI nr
Match: KAE8647505.1 (hypothetical protein Csa_003084 [Cucumis sativus])

HSP 1 Score: 191.0 bits (484), Expect = 4.8e-45
Identity = 97/100 (97.00%), Postives = 98/100 (98.00%), Query Frame = 0


Query: 1   MRSITNIFIGFILVAALLGNGGLVIVTDARPLTLVGLGSGSAATIAEDFFEGLSLGAIKQ 60
           MRSITNIFIGFILVAALLGNGGLV VTDARPLTL+ LGSGSAATIAEDFFEGLSLGAIKQ
Sbjct: 7   MRSITNIFIGFILVAALLGNGGLVTVTDARPLTLMRLGSGSAATIAEDFFEGLSLGAIKQ 66

Query: 61  SGPSAGGDGHKFVNYDTLGGIKDSGPAPGDGHRHITSSHH 101
           SGPSAGGDGHKFVNYDTLGGIKDSGPAPGDGHRHITSSHH
Sbjct: 67  SGPSAGGDGHKFVNYDTLGGIKDSGPAPGDGHRHITSSHH 106

[Close]

BLAST of CSPI06G28430 vs. NCBI nr
Match: TYK12857.1 (hypothetical protein E5676_scaffold255G004490 [Cucumis melo var. makuwa])

HSP 1 Score: 157.5 bits (397), Expect = 5.9e-35
Identity = 80/97 (82.47%), Postives = 86/97 (88.66%), Query Frame = 0


Query: 4   ITNIFIGFILVAALLGNGGLVIVTDARPLTLVGLGSGSAATIAEDFFEGLSLGAIKQSGP 63
           ++ IFIG ILV ALL NGGLVIVT+ARPL L  +GSGSAA IAEDFFEGLSLGAIKQSGP
Sbjct: 1   MSKIFIGLILVVALLDNGGLVIVTEARPLGL--MGSGSAAAIAEDFFEGLSLGAIKQSGP 60

Query: 64  SAGGDGHKFVNYDTLGGIKDSGPAPGDGHRHITSSHH 101
           SAGGDGHKFVNYDT GG+KDSGP PGDGH H+TSSHH
Sbjct: 61  SAGGDGHKFVNYDTFGGMKDSGPTPGDGHSHVTSSHH 95

[Close]

BLAST of CSPI06G28430 vs. NCBI nr
Match: KAG6595008.1 (PAMP-induced secreted peptide 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027036.1 hypothetical protein SDJN02_11045, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 133.3 bits (334), Expect = 1.2e-27
Identity = 72/99 (72.73%), Postives = 82/99 (82.83%), Query Frame = 0


Query: 1  MRSITNIFIGFILVAALL-GNGGLVIVTDARPLTLVGLGSGSAATIAEDFFEGLSLGAIK 60
          MR++  + IG ILVAALL GNGGLV  T+ARPL  +     ++A IAEDFFEGLSLGAIK
Sbjct: 1  MRNVGKLLIGLILVAALLAGNGGLV-ATEARPLNAM-----ASAAIAEDFFEGLSLGAIK 60

Query: 61 QSGPSAGGDGHKFVNYDTLGGIKDSGPAPGDGHRHITSS 99
          QSGPS GGDGHKFVN+DTLGGIK+SGP+PGDGH HITSS
Sbjct: 61 QSGPSPGGDGHKFVNFDTLGGIKESGPSPGDGHSHITSS 93

[Close]

BLAST of CSPI06G28430 vs. NCBI nr
Match: KAF7828678.1 (PAMP-induced secreted peptide 2-like [Senna tora])

HSP 1 Score: 87.4 bits (215), Expect = 7.6e-14
Identity = 49/85 (57.65%), Postives = 64/85 (75.29%), Query Frame = 0


Query: 17  LLGNGGLVIVTDARPLTLV-GLGSGSAATIAEDFFEGLSLGAIKQSGPSAGGDGHKFVNY 76
           LL N   V+V +ARPL+++  + SG+   +  DFF+ LSLGAIKQSGPS GG+GHKF N 
Sbjct: 6   LLVNTAFVVVLEARPLSIIKEVSSGTRDEV--DFFDWLSLGAIKQSGPSGGGEGHKFTNT 65

Query: 77  DTLGGIKDSGPAP-GDGHRHITSSH 100
           +TLGGIKDSGP+P G GH+  T++H
Sbjct: 66  ETLGGIKDSGPSPSGPGHK-FTNTH 87

[Close]

BLAST of CSPI06G28430 vs. NCBI nr
Match: XP_030477329.1 (PAMP-induced secreted peptide 2-like isoform X2 [Syzygium oleosum])

HSP 1 Score: 83.6 bits (205), Expect = 1.1e-12
Identity = 45/98 (45.92%), Postives = 62/98 (63.27%), Query Frame = 0


Query: 1  MRSITNIFIGFILVAALLGNGGLVIVTDARPLTLVGLGSGSAATIAEDFFEGLSLGAIKQ 60
          MR+ T     F+++A L     + +  +ARP  ++             FF+GLSLGAIKQ
Sbjct: 1  MRTATRALHAFLVLAML---SAIFVANEARPFNVMET-KAPVGVACGGFFDGLSLGAIKQ 60

Query: 61 SGPSAGGDGHKFVNYDTLGGIKDSGPAPGDGHRHITSS 99
          +GPS  G+GHKF N +TLGGIKDSGP+PG+GH H+T +
Sbjct: 61 AGPSP-GEGHKFTNSETLGGIKDSGPSPGEGHGHVTGT 93

[Close]

BLAST of CSPI06G28430 vs. TAIR 10
Match: AT2G23270.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: stem, sperm cell, root, stamen; EXPRESSED DURING: 4 anthesis; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G37290.1); Has 36 Blast hits to 35 proteins in 6 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 36; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 55.8 bits (133), Expect = 2.3e-08
Identity = 41/87 (47.13%), Postives = 56/87 (64.37%), Query Frame = 0


Query: 8  FIGFILVAALLGNGGLVIVTDARPLTLVGLGSGSAATIAEDFFEGLSLGAIKQSGPSAGG 67
          F+ F+LV++         V +ARPL   GL       +A+ FF+GLSLGAIK+SGPS+GG
Sbjct: 11 FLFFMLVSS-------NFVVEARPL---GLTKAEENLVAK-FFDGLSLGAIKESGPSSGG 70

Query: 68 DGHKFVN-YDTLGGIKDSGPA-PGDGH 93
          +GH+FV+  +TL   K SGP+  G GH
Sbjct: 71 EGHRFVDRTETLEYGKHSGPSTSGPGH 86

[Close]

BLAST of CSPI06G28430 vs. TAIR 10
Match: AT4G37290.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: response to karrikin; LOCATED IN: endomembrane system; EXPRESSED IN: cotyledon, hypocotyl, leaf; EXPRESSED DURING: LP.04 four leaves visible; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G23270.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 47.8 bits (112), Expect = 6.2e-06
Identity = 36/83 (43.37%), Postives = 50/83 (60.24%), Query Frame = 0


Query: 12 ILVAALLGNGGLVIVTDARPLTLVGLGSGSAATIAEDFFEGLSLGAIKQSGPSAGGDGHK 71
          IL   L+G+    ++ ++RPL L        A++    F+GLSLG+IK SGPS  G+GHK
Sbjct: 11 ILFFMLIGS----VLVESRPLGLTKTEEKFVASL----FDGLSLGSIKDSGPSP-GEGHK 70

Query: 72 FVN-YDTLGGIKDSGPAP-GDGH 93
           V+  DT   +K SGP+P G GH
Sbjct: 71 VVDRKDTFRFVKHSGPSPSGPGH 84

The following BLAST results are available for this feature:

BLAST of CSPI06G28430 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JRC5	8.7e-05	43.37	PAMP-induced secreted peptide 2 OS=Arabidopsis thaliana OX=3702 GN=PIP2 PE=1 SV=...	[more]

BLAST of CSPI06G28430 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KIE2	2.3e-45	97.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G491710 PE=4 SV=1	[more]
A0A5D3CLN9	2.9e-35	82.47	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A059AZU8	1.2e-12	47.47	Uncharacterized protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_H02229 PE=4 SV...	[more]
V7CCF8	3.4e-12	48.98	Uncharacterized protein OS=Phaseolus vulgaris OX=3885 GN=PHAVU_003G239800g PE=4 ...	[more]
D7TTE3	7.6e-12	46.24	Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_07s0129g01010 PE=4 SV=...	[more]

BLAST of CSPI06G28430 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8647505.1	4.8e-45	97.00	hypothetical protein Csa_003084 [Cucumis sativus]	[more]
TYK12857.1	5.9e-35	82.47	hypothetical protein E5676_scaffold255G004490 [Cucumis melo var. makuwa]	[more]
KAG6595008.1	1.2e-27	72.73	PAMP-induced secreted peptide 2, partial [Cucurbita argyrosperma subsp. sororia]...	[more]
KAF7828678.1	7.6e-14	57.65	PAMP-induced secreted peptide 2-like [Senna tora]	[more]
XP_030477329.1	1.1e-12	45.92	PAMP-induced secreted peptide 2-like isoform X2 [Syzygium oleosum]	[more]

BLAST of CSPI06G28430 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G23270.1	2.3e-08	47.13	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]
AT4G37290.1	6.2e-06	43.37	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: response...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044700	PIP2/PIPL1	PANTHER	PTHR34663	OS06G0637400 PROTEIN	coord: 9..99

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI06G28430.1	CSPI06G28430.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045087	innate immune response
biological_process	GO:0050793	regulation of developmental process