CSPI05G21680 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI05G21680
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Mitotic spindle checkpoint protein MAD2
Location	Chr5: 22362973 .. 22364967 (+)
RNA-Seq Expression	CSPI05G21680
Synteny	CSPI05G21680

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCCTTCGTCTTTTACTTTTAATCACTTTCTGCGTCTCAAAGTTGTCAAATTTTCTTAGTTCCTCTCCTTTTTCTCTTTGTCCATTTGATTTCTTGATTCATCGACTCCAATTTTTCTTTCAAATTTGGTTTCCCTCTGTTTCGATTACTAGCCCTAAATCCGCTCAATCCAAGATTTGAAATGGCTTCGAAAACAGCGACCAAAGACATAATCACTCTTCGCGGTTCTGCGGCAATTGTCAGCGAGTTCTTCGGTATATTTATTGCAATTTTCTGTTTAATATGGTTGATGATTGATGAATAATATTGGCTGATTTTTTTTCCTTTTTGTTTTCTTCAATTAGGGTATGCTGCGAATAGGTATGAATACTGGGTGTTCTTTCTTTATTCTTATTTTTGTCTTTATGATCGTGTAAATTTGGAATTTTTCACTTACGAGAGATGTCGATCGTAACTGTGTAGCATCCTTTACAATCGTGGGCTTTATCCGGAGGAAAGTTTTGTTAGGGTTAAGAAATATGGACTTCCTATGTTGCTTACTCAAGATGAAGGTGTTAAGACCTTCATTTCTAATCTAACTGCTCAGCTTTCTGGTTAGTTCCCTTTCTATGATTGAAAGATTCGTAACTTCTAACAGTTCAGAATTGTGTTTTTTGTTTCTTTTTTCTCTTGCAGTAGATCAGGTTGTAGTGTGATTTATACTTTCATGAGAACTGTATTTATTGGTGAGTTTTTGAATTGTGAAATTGGTTTTAGAATGGCTGGAAGCTGGGAAGTTACAAAGGGTTGTGCTCGTTATAATGAGCAAATCCAACAATGAGGTCCTTGAGAGGTGGAATTTTAGAATTGAAACTGATGGGGAGGTGGTGGAACAAGGGTAATTCAACGAAAAACTCCTCTTCCCCCTTTCCTAAATTTAGTGATCTTGTGTTGATCAGATGACATTGTTGCAGTGTATCGAGGGAAAAGAGTGATAAAGAAATAATGAGGGAGATCCAAGCAATTATGAGACAGATTGCTTCAAGCATCACCTACTTGCCCTGCCTAGATGAACCCTGTAAGTGCTGACAAAATACATCATTTTACGGATTGGTATAACATGATGTGTGATGTTGCTTGCCCACGTTCTTCTAAGTCCATAGGATGTCTTACCAATATGAAATCATTTAGCAGATCCTGTAAGTTTTTCTCCAATCATCACTTGCTCTGCTAGATGATGAGCTGTAGGGGGTTAGCAAGATTACCATGATGCTGCATTTGCTATTTTGGGGATTAGTTTCACATATGAAGCCTTATATTTTAGGCTGAGTTATGAATCAGCGGGATGTCACAATAGTTCCACCGTGAAATCGTTACTTTTTATAGATGATTTGAACATAGCTTTGAATCCATGCATAGGGTGTATCTTTCTTATGGGATTTAAGTTCAAATCATGTCCTCAATTAAGTGTTCTCGATGTTTTTGTTGTCTCAGGTGTATTCGATGTGTTAGCATATACTGATAAAGATCTTGCAGTTCCATTTACTTGGATGGAGAGTGACCCTAAACTGATTGCTAATCCACAAATGGTGAAGCTGCATTCTTTTGACACAAAGGTGAGAGAATGGGACTTCGTGCAAGCAAATAAGATCAAACTTATTCGTTCAACTAATCTCGATCCACTGCAAGCAAATAATTAAACTTTTAAATGGTGTGTGTATTGCAGATTCACAAAGTGGACACTCTTGTTTCTTACAAGAATGATGATGACGACGACGAGTAAACACGAAAGTGAACTTCATCTTCTTCAATTTGTTGATGGTTTCTGAATCCTCCAACCTGATCTCTCGCTTTCCAAGGTGGCATTGTGAATCATTTATTCACATTTTGTATTGTACTCTAACACTGGAATGAACAATGTTGATTTGTGTGTTCGATTAGAATTCCACCTAAATGTAATGGTTGAAGATTGTTATTGTTTCTCTATAAAGAAAACGTGTTCTCGACAAGAACAAGG

mRNA sequence

CCCTTCGTCTTTTACTTTTAATCACTTTCTGCGTCTCAAAGTTGTCAAATTTTCTTAGTTCCTCTCCTTTTTCTCTTTGTCCATTTGATTTCTTGATTCATCGACTCCAATTTTTCTTTCAAATTTGGTTTCCCTCTGTTTCGATTACTAGCCCTAAATCCGCTCAATCCAAGATTTGAAATGGCTTCGAAAACAGCGACCAAAGACATAATCACTCTTCGCGGTTCTGCGGCAATTGTCAGCGAGTTCTTCGGGTATGCTGCGAATAGCATCCTTTACAATCGTGGGCTTTATCCGGAGGAAAGTTTTGTTAGGGTTAAGAAATATGGACTTCCTATGTTGCTTACTCAAGATGAAGGTGTTAAGACCTTCATTTCTAATCTAACTGCTCAGCTTTCTGAATGGCTGGAAGCTGGGAAGTTACAAAGGGTTGTGCTCGTTATAATGAGCAAATCCAACAATGAGGTCCTTGAGAGGTGGAATTTTAGAATTGAAACTGATGGGGAGGTGGTGGAACAAGGTGTATCGAGGGAAAAGAGTGATAAAGAAATAATGAGGGAGATCCAAGCAATTATGAGACAGATTGCTTCAAGCATCACCTACTTGCCCTGCCTAGATGAACCCTGTGTATTCGATGTGTTAGCATATACTGATAAAGATCTTGCAGTTCCATTTACTTGGATGGAGAGTGACCCTAAACTGATTGCTAATCCACAAATGGTGAAGCTGCATTCTTTTGACACAAAGATTCACAAAGTGGACACTCTTGTTTCTTACAAGAATGATGATGACGACGACGAGTAAACACGAAAGTGAACTTCATCTTCTTCAATTTGTTGATGGTTTCTGAATCCTCCAACCTGATCTCTCGCTTTCCAAGGTGGCATTGTGAATCATTTATTCACATTTTGTATTGTACTCTAACACTGGAATGAACAATGTTGATTTGTGTGTTCGATTAGAATTCCACCTAAATGTAATGGTTGAAGATTGTTATTGTTTCTCTATAAAGAAAACGTGTTCTCGACAAGAACAAGG

Coding sequence (CDS)

ATGGCTTCGAAAACAGCGACCAAAGACATAATCACTCTTCGCGGTTCTGCGGCAATTGTCAGCGAGTTCTTCGGGTATGCTGCGAATAGCATCCTTTACAATCGTGGGCTTTATCCGGAGGAAAGTTTTGTTAGGGTTAAGAAATATGGACTTCCTATGTTGCTTACTCAAGATGAAGGTGTTAAGACCTTCATTTCTAATCTAACTGCTCAGCTTTCTGAATGGCTGGAAGCTGGGAAGTTACAAAGGGTTGTGCTCGTTATAATGAGCAAATCCAACAATGAGGTCCTTGAGAGGTGGAATTTTAGAATTGAAACTGATGGGGAGGTGGTGGAACAAGGTGTATCGAGGGAAAAGAGTGATAAAGAAATAATGAGGGAGATCCAAGCAATTATGAGACAGATTGCTTCAAGCATCACCTACTTGCCCTGCCTAGATGAACCCTGTGTATTCGATGTGTTAGCATATACTGATAAAGATCTTGCAGTTCCATTTACTTGGATGGAGAGTGACCCTAAACTGATTGCTAATCCACAAATGGTGAAGCTGCATTCTTTTGACACAAAGATTCACAAAGTGGACACTCTTGTTTCTTACAAGAATGATGATGACGACGACGAGTAA

Protein sequence

MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEGVKTFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDDDDDE*

Homology

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BLAST of CSPI05G21680 vs. ExPASy Swiss-Prot
Match: Q9LU93 (Mitotic spindle checkpoint protein MAD2 OS=Arabidopsis thaliana OX=3702 GN=MAD2 PE=1 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 3.4e-96
Identity = 178/208 (85.58%), Postives = 194/208 (93.27%), Query Frame = 0


Query: 1   MASKT-ATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDE 60
           MASKT A KDIITL GSAAIVSEFF YAANSILYNR +YPEESFV+VKKYGLPMLL +DE
Sbjct: 1   MASKTAAAKDIITLHGSAAIVSEFFCYAANSILYNRAVYPEESFVKVKKYGLPMLLIEDE 60

Query: 61  GVKTFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREK 120
            VK+F+SNLT+Q+SEWLEAGKLQRVVLVIMSK+  EVLERWNFRIETD EVV++GVSREK
Sbjct: 61  SVKSFMSNLTSQISEWLEAGKLQRVVLVIMSKATGEVLERWNFRIETDNEVVDKGVSREK 120

Query: 121 SDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQ 180
           SDKEIMREIQAIMRQ+ASS+TYLPCLDE CVFDVLAYTD D+AVPFTW+ESDPKLIANPQ
Sbjct: 121 SDKEIMREIQAIMRQVASSVTYLPCLDETCVFDVLAYTDTDVAVPFTWIESDPKLIANPQ 180

Query: 181 MVKLHSFDTKIHKVDTLVSYKNDDDDDE 208
           MVKLH FDTKIHKVDTLVSYKND+ D+E
Sbjct: 181 MVKLHGFDTKIHKVDTLVSYKNDEWDEE 208

[Close]

BLAST of CSPI05G21680 vs. ExPASy Swiss-Prot
Match: Q9XFH3 (Mitotic spindle checkpoint protein MAD2 OS=Zea mays OX=4577 GN=MAD2 PE=2 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 3.9e-92
Identity = 171/207 (82.61%), Postives = 188/207 (90.82%), Query Frame = 0


Query: 1   MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60
           MAS++A+KDIITLRGSAAIVSEFFGYAANSILYNR +YPEESF +VKKYGL MLLTQDEG
Sbjct: 1   MASRSASKDIITLRGSAAIVSEFFGYAANSILYNRAVYPEESFSKVKKYGLTMLLTQDEG 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS 120
           VK FI++LT+QLSEWLEAGKLQR+VLVIMSK+ +EVLERWNF I TD EVVE+G  +EKS
Sbjct: 61  VKNFIASLTSQLSEWLEAGKLQRIVLVIMSKATSEVLERWNFNIVTDAEVVEKGAIKEKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQM 180
           DKEIMREIQAIMRQIAS ITYLPCLDEPCVFDVLAYTD D+  P TW+ESD KLI NPQM
Sbjct: 121 DKEIMREIQAIMRQIASCITYLPCLDEPCVFDVLAYTDTDVDAPGTWVESDAKLIDNPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDDDDE 208
           VKLHSFDTKIHKVDTLVSYK D+ D+E
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKKDEWDEE 207

[Close]

BLAST of CSPI05G21680 vs. ExPASy Swiss-Prot
Match: Q556Y9 (Mitotic spindle assembly checkpoint protein MAD2A OS=Dictyostelium discoideum OX=44689 GN=mad2l1-1 PE=3 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 2.0e-56
Identity = 112/205 (54.63%), Postives = 147/205 (71.71%), Query Frame = 0


Query: 1   MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60
           M +  A+K  I+L+GS  IV+EFF Y+ N+IL+ RGLYP ESF RV KYGLP+L+T D+ 
Sbjct: 1   MQAAVASKTNISLKGSTEIVTEFFSYSINTILFQRGLYPPESFTRVAKYGLPILVTNDQS 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQG-VSREK 120
           +K ++ N+  QLSEWL +G +Q++VLVI      EVLERW F + TD  + ++G   R+K
Sbjct: 61  LKDYLDNVLKQLSEWLLSGDVQKLVLVITDIVTKEVLERWVFDVTTD--IPKEGEAPRQK 120

Query: 121 SDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQ 180
            +KEIM EIQAI+RQI +S+T+LP L   C FD+L YT KDLAVP  W ESDPK I N Q
Sbjct: 121 PEKEIMNEIQAIIRQITASVTFLPLLPNACTFDLLVYTSKDLAVPQKWEESDPKFITNSQ 180

Query: 181 MVKLHSFDTKIHKVDTLVSYKNDDD 205
            VKL SF T IHKV+++V+YK  +D
Sbjct: 181 QVKLRSFTTTIHKVESMVAYKISND 203

[Close]

BLAST of CSPI05G21680 vs. ExPASy Swiss-Prot
Match: Q9Z1B5 (Mitotic spindle assembly checkpoint protein MAD2A OS=Mus musculus OX=10090 GN=Mad2l1 PE=1 SV=2)

HSP 1 Score: 206.8 bits (525), Expect = 2.3e-52
Identity = 104/205 (50.73%), Postives = 142/205 (69.27%), Query Frame = 0


Query: 1   MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60
           MA + A +  ITLRGSA IV+EFF +  NSILY RG+YP E+F RV+KYGL +L T D  
Sbjct: 1   MAQQLAREQGITLRGSAEIVAEFFSFGINSILYQRGIYPSETFTRVQKYGLTLLTTTDPE 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS 120
           +  +++N+  QL EWL    +Q++V+VI +  + EVLERW F IE D    E+GV REKS
Sbjct: 61  LIKYLNNVVEQLKEWLYKCSVQKLVVVISNIESGEVLERWQFDIECDKTAKEEGVRREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQM 180
            K I  EI++++RQI +++T+LP L+  C FD+L YTDKDL VP  W ES P+ I N + 
Sbjct: 121 QKAIQDEIRSVIRQITATVTFLPLLEVSCSFDLLIYTDKDLVVPEKWEESGPQFITNCEE 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDDD 206
           V+L SF T IHKV+++V+YK   +D
Sbjct: 181 VRLRSFTTTIHKVNSMVAYKTPVND 205

[Close]

BLAST of CSPI05G21680 vs. ExPASy Swiss-Prot
Match: Q13257 (Mitotic spindle assembly checkpoint protein MAD2A OS=Homo sapiens OX=9606 GN=MAD2L1 PE=1 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 2.8e-50
Identity = 98/200 (49.00%), Postives = 138/200 (69.00%), Query Frame = 0


Query: 1   MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60
           MA + + +  ITLRGSA IV+EFF +  NSILY RG+YP E+F RV+KYGL +L+T D  
Sbjct: 1   MALQLSREQGITLRGSAEIVAEFFSFGINSILYQRGIYPSETFTRVQKYGLTLLVTTDLE 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS 120
           +  +++N+  QL +WL    +Q++V+VI +  + EVLERW F IE D    +    REKS
Sbjct: 61  LIKYLNNVVEQLKDWLYKCSVQKLVVVISNIESGEVLERWQFDIECDKTAKDDSAPREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQM 180
            K I  EI++++RQI +++T+LP L+  C FD+L YTDKDL VP  W ES P+ I N + 
Sbjct: 121 QKAIQDEIRSVIRQITATVTFLPLLEVSCSFDLLIYTDKDLVVPEKWEESGPQFITNSEE 180

Query: 181 VKLHSFDTKIHKVDTLVSYK 201
           V+L SF T IHKV+++V+YK
Sbjct: 181 VRLRSFTTTIHKVNSMVAYK 200

[Close]

BLAST of CSPI05G21680 vs. ExPASy TrEMBL
Match: A0A0A0KPY5 (HORMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G590170 PE=3 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 3.3e-110
Identity = 207/207 (100.00%), Postives = 207/207 (100.00%), Query Frame = 0


Query: 1   MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60
           MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG
Sbjct: 1   MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS 120
           VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS
Sbjct: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQM 180
           DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQM
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDDDDE 208
           VKLHSFDTKIHKVDTLVSYKNDDDDDE
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKNDDDDDE 207

[Close]

BLAST of CSPI05G21680 vs. ExPASy TrEMBL
Match: A0A5A7STC2 (Mitotic spindle checkpoint protein MAD2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G002160 PE=3 SV=1)

HSP 1 Score: 402.5 bits (1033), Expect = 1.1e-108
Identity = 204/205 (99.51%), Postives = 205/205 (100.00%), Query Frame = 0


Query: 1   MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60
           MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG
Sbjct: 1   MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS 120
           VK+FISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS
Sbjct: 61  VKSFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQM 180
           DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQM
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDDD 206
           VKLHSFDTKIHKVDTLVSYKNDDDD
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKNDDDD 205

[Close]

BLAST of CSPI05G21680 vs. ExPASy TrEMBL
Match: A0A5D3CWF2 (Mitotic spindle checkpoint protein MAD2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001990 PE=3 SV=1)

HSP 1 Score: 402.1 bits (1032), Expect = 1.4e-108
Identity = 204/207 (98.55%), Postives = 206/207 (99.52%), Query Frame = 0


Query: 1   MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60
           MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG
Sbjct: 1   MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS 120
           VK+FISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS
Sbjct: 61  VKSFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQM 180
           DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQM
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDDDDE 208
           VKLHSFDTKIHKVDTLVSYKNDDD D+
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKNDDDGDD 207

[Close]

BLAST of CSPI05G21680 vs. ExPASy TrEMBL
Match: A0A1S3BDR9 (mitotic spindle checkpoint protein MAD2 OS=Cucumis melo OX=3656 GN=LOC103488916 PE=3 SV=1)

HSP 1 Score: 402.1 bits (1032), Expect = 1.4e-108
Identity = 204/207 (98.55%), Postives = 206/207 (99.52%), Query Frame = 0


Query: 1   MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60
           MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG
Sbjct: 1   MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS 120
           VK+FISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS
Sbjct: 61  VKSFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQM 180
           DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQM
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDDDDE 208
           VKLHSFDTKIHKVDTLVSYKNDDD D+
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKNDDDGDD 207

[Close]

BLAST of CSPI05G21680 vs. ExPASy TrEMBL
Match: A0A6J1HTK5 (mitotic spindle checkpoint protein MAD2 OS=Cucurbita maxima OX=3661 GN=LOC111466640 PE=3 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 2.0e-107
Identity = 202/207 (97.58%), Postives = 205/207 (99.03%), Query Frame = 0


Query: 1   MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60
           MASKTA+KDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESF RVKKYGLPMLLTQDEG
Sbjct: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS 120
           VKTFISNLTAQLSEWLEAGKLQRVVLVIMSK+NNEVLERWNFRIETDGEVVEQGVSREKS
Sbjct: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGEVVEQGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQM 180
           DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTW+ESDPKLIANPQM
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDDDDE 208
           VKLHSFDTKIHKVDTLVSYKNDD DDE
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKNDDCDDE 207

[Close]

BLAST of CSPI05G21680 vs. NCBI nr
Match: KAE8648685.1 (hypothetical protein Csa_009142 [Cucumis sativus])

HSP 1 Score: 407.5 bits (1046), Expect = 6.8e-110
Identity = 207/207 (100.00%), Postives = 207/207 (100.00%), Query Frame = 0


Query: 1   MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60
           MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG
Sbjct: 67  MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 126

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS 120
           VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS
Sbjct: 127 VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS 186

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQM 180
           DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQM
Sbjct: 187 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQM 246

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDDDDE 208
           VKLHSFDTKIHKVDTLVSYKNDDDDDE
Sbjct: 247 VKLHSFDTKIHKVDTLVSYKNDDDDDE 273

[Close]

BLAST of CSPI05G21680 vs. NCBI nr
Match: XP_004135301.1 (mitotic spindle checkpoint protein MAD2 [Cucumis sativus])

HSP 1 Score: 407.5 bits (1046), Expect = 6.8e-110
Identity = 207/207 (100.00%), Postives = 207/207 (100.00%), Query Frame = 0


Query: 1   MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60
           MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG
Sbjct: 1   MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS 120
           VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS
Sbjct: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQM 180
           DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQM
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDDDDE 208
           VKLHSFDTKIHKVDTLVSYKNDDDDDE
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKNDDDDDE 207

[Close]

BLAST of CSPI05G21680 vs. NCBI nr
Match: KAA0034220.1 (mitotic spindle checkpoint protein MAD2 [Cucumis melo var. makuwa])

HSP 1 Score: 402.5 bits (1033), Expect = 2.2e-108
Identity = 204/205 (99.51%), Postives = 205/205 (100.00%), Query Frame = 0


Query: 1   MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60
           MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG
Sbjct: 1   MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS 120
           VK+FISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS
Sbjct: 61  VKSFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQM 180
           DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQM
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDDD 206
           VKLHSFDTKIHKVDTLVSYKNDDDD
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKNDDDD 205

[Close]

BLAST of CSPI05G21680 vs. NCBI nr
Match: XP_008446088.1 (PREDICTED: mitotic spindle checkpoint protein MAD2 [Cucumis melo])

HSP 1 Score: 402.1 bits (1032), Expect = 2.9e-108
Identity = 204/207 (98.55%), Postives = 206/207 (99.52%), Query Frame = 0


Query: 1   MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60
           MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG
Sbjct: 1   MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS 120
           VK+FISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS
Sbjct: 61  VKSFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQM 180
           DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQM
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDDDDE 208
           VKLHSFDTKIHKVDTLVSYKNDDD D+
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKNDDDGDD 207

[Close]

BLAST of CSPI05G21680 vs. NCBI nr
Match: TYK15700.1 (mitotic spindle checkpoint protein MAD2 [Cucumis melo var. makuwa])

HSP 1 Score: 402.1 bits (1032), Expect = 2.9e-108
Identity = 204/207 (98.55%), Postives = 206/207 (99.52%), Query Frame = 0


Query: 1   MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60
           MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG
Sbjct: 1   MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS 120
           VK+FISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS
Sbjct: 61  VKSFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQM 180
           DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQM
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDDDDE 208
           VKLHSFDTKIHKVDTLVSYKNDDD D+
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKNDDDGDD 207

[Close]

BLAST of CSPI05G21680 vs. TAIR 10
Match: AT3G25980.1 (DNA-binding HORMA family protein )

HSP 1 Score: 352.4 bits (903), Expect = 2.4e-97
Identity = 178/208 (85.58%), Postives = 194/208 (93.27%), Query Frame = 0


Query: 1   MASKT-ATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDE 60
           MASKT A KDIITL GSAAIVSEFF YAANSILYNR +YPEESFV+VKKYGLPMLL +DE
Sbjct: 1   MASKTAAAKDIITLHGSAAIVSEFFCYAANSILYNRAVYPEESFVKVKKYGLPMLLIEDE 60

Query: 61  GVKTFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREK 120
            VK+F+SNLT+Q+SEWLEAGKLQRVVLVIMSK+  EVLERWNFRIETD EVV++GVSREK
Sbjct: 61  SVKSFMSNLTSQISEWLEAGKLQRVVLVIMSKATGEVLERWNFRIETDNEVVDKGVSREK 120

Query: 121 SDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQ 180
           SDKEIMREIQAIMRQ+ASS+TYLPCLDE CVFDVLAYTD D+AVPFTW+ESDPKLIANPQ
Sbjct: 121 SDKEIMREIQAIMRQVASSVTYLPCLDETCVFDVLAYTDTDVAVPFTWIESDPKLIANPQ 180

Query: 181 MVKLHSFDTKIHKVDTLVSYKNDDDDDE 208
           MVKLH FDTKIHKVDTLVSYKND+ D+E
Sbjct: 181 MVKLHGFDTKIHKVDTLVSYKNDEWDEE 208

[Close]

BLAST of CSPI05G21680 vs. TAIR 10
Match: AT3G25980.2 (DNA-binding HORMA family protein )

HSP 1 Score: 337.4 bits (864), Expect = 8.0e-93
Identity = 173/208 (83.17%), Postives = 190/208 (91.35%), Query Frame = 0


Query: 1   MASKT-ATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDE 60
           MASKT A KDIITL GSAAIVSEFF     +ILYNR +YPEESFV+VKKYGLPMLL +DE
Sbjct: 1   MASKTAAAKDIITLHGSAAIVSEFF----CTILYNRAVYPEESFVKVKKYGLPMLLIEDE 60

Query: 61  GVKTFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREK 120
            VK+F+SNLT+Q+SEWLEAGKLQRVVLVIMSK+  EVLERWNFRIETD EVV++GVSREK
Sbjct: 61  SVKSFMSNLTSQISEWLEAGKLQRVVLVIMSKATGEVLERWNFRIETDNEVVDKGVSREK 120

Query: 121 SDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQ 180
           SDKEIMREIQAIMRQ+ASS+TYLPCLDE CVFDVLAYTD D+AVPFTW+ESDPKLIANPQ
Sbjct: 121 SDKEIMREIQAIMRQVASSVTYLPCLDETCVFDVLAYTDTDVAVPFTWIESDPKLIANPQ 180

Query: 181 MVKLHSFDTKIHKVDTLVSYKNDDDDDE 208
           MVKLH FDTKIHKVDTLVSYKND+ D+E
Sbjct: 181 MVKLHGFDTKIHKVDTLVSYKNDEWDEE 204

The following BLAST results are available for this feature:

BLAST of CSPI05G21680 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LU93	3.4e-96	85.58	Mitotic spindle checkpoint protein MAD2 OS=Arabidopsis thaliana OX=3702 GN=MAD2 ...	[more]
Q9XFH3	3.9e-92	82.61	Mitotic spindle checkpoint protein MAD2 OS=Zea mays OX=4577 GN=MAD2 PE=2 SV=1	[more]
Q556Y9	2.0e-56	54.63	Mitotic spindle assembly checkpoint protein MAD2A OS=Dictyostelium discoideum OX...	[more]
Q9Z1B5	2.3e-52	50.73	Mitotic spindle assembly checkpoint protein MAD2A OS=Mus musculus OX=10090 GN=Ma...	[more]
Q13257	2.8e-50	49.00	Mitotic spindle assembly checkpoint protein MAD2A OS=Homo sapiens OX=9606 GN=MAD...	[more]

BLAST of CSPI05G21680 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KPY5	3.3e-110	100.00	HORMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G590170 PE=3 ...	[more]
A0A5A7STC2	1.1e-108	99.51	Mitotic spindle checkpoint protein MAD2 OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A5D3CWF2	1.4e-108	98.55	Mitotic spindle checkpoint protein MAD2 OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A1S3BDR9	1.4e-108	98.55	mitotic spindle checkpoint protein MAD2 OS=Cucumis melo OX=3656 GN=LOC103488916 ...	[more]
A0A6J1HTK5	2.0e-107	97.58	mitotic spindle checkpoint protein MAD2 OS=Cucurbita maxima OX=3661 GN=LOC111466...	[more]

BLAST of CSPI05G21680 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8648685.1	6.8e-110	100.00	hypothetical protein Csa_009142 [Cucumis sativus]	[more]
XP_004135301.1	6.8e-110	100.00	mitotic spindle checkpoint protein MAD2 [Cucumis sativus]	[more]
KAA0034220.1	2.2e-108	99.51	mitotic spindle checkpoint protein MAD2 [Cucumis melo var. makuwa]	[more]
XP_008446088.1	2.9e-108	98.55	PREDICTED: mitotic spindle checkpoint protein MAD2 [Cucumis melo]	[more]
TYK15700.1	2.9e-108	98.55	mitotic spindle checkpoint protein MAD2 [Cucumis melo var. makuwa]	[more]

BLAST of CSPI05G21680 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G25980.1	2.4e-97	85.58	DNA-binding HORMA family protein	[more]
AT3G25980.2	8.0e-93	83.17	DNA-binding HORMA family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036570	HORMA domain superfamily	GENE3D	3.30.900.10	HORMA domain	coord: 1..204 e-value: 4.1E-78 score: 263.5
IPR036570	HORMA domain superfamily	SUPERFAMILY	56019	The spindle assembly checkpoint protein mad2	coord: 10..202
IPR003511	HORMA domain	PFAM	PF02301	HORMA	coord: 16..191 e-value: 3.1E-20 score: 72.7
IPR003511	HORMA domain	PROSITE	PS50815	HORMA	coord: 14..197 score: 45.824375
IPR027097	Mitotic spindle checkpoint protein Mad2	PANTHER	PTHR11842:SF11	MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2A	coord: 1..204
IPR045091	Mad2-like	PANTHER	PTHR11842	MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2	coord: 1..204

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI05G21680.1	CSPI05G21680.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051301	cell division
biological_process	GO:0007094	mitotic spindle assembly checkpoint signaling
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0000776	kinetochore
cellular_component	GO:0005654	nucleoplasm
molecular_function	GO:0003677	DNA binding