CSPI01G23840 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G23840
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description3-oxo-Delta(4,5)-steroid 5-beta-reductase-like
LocationChr1: 19311415 .. 19312920 (-)
RNA-Seq ExpressionCSPI01G23840
SyntenyCSPI01G23840
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGTCGAAATAATCAACACAAATTTGGATGCCAATGGCGAACTCGTCGCTGTCATATTTGGAGTCACCGGTTTGGTCGGAAAACAGTTGGCCAAAACCCTTCTCTCCACCGCCGGCTGGAAAGTCTACGGCGTCGCACGGCGGCCGGACAATGTCTCTCCCATCTCCCATCCCAAATTTCATTTCATCTCTTGTGATTTGCTCGACCGCCGGTCCGTTCAACAGAATCTCTCTCCCGTTCGCCACGTCACCCACCTTTTTTGGATCACTTGGGCAGCTCAGCTCCGGTTAGATTCCCCGGATTGCTCCGATCAGAATCGGGCCATGTTGGCCAACGCCCTCGACGCCATTCTCCCGTCCGCGCCGGCCCTCCGCCACGTCTCTCTACAGACCGGGATCAAACATTATGCTTCGTTGACGAGACTCGCCGCTGGCTGCGGCGGCGGAGGAGAAGGGGAGGTGGTTTATTGTGAGGATAGTCCGAGAGCGGAATCTGGGAACAATTTTTATTACGTGTTGGAAGATCTCCTCCGTGAGCGGTTGTCCTGTGGGCGGAGAATGGTGGCGTGGAGTGTTCTCCGACCCGGTTTGATATTGGGATGTTCGAACCGAACTTTCTTTAATTTTATGGGGAGTTTGTGTGTTTATGGTGCCATCTGTAAGAAGTTGAAGATGCCATTTGTGTTCGGCGGCACGGCGGCTTGCTGGGAGGAGGTTTATATCGACGGTTCCGATGCTCGACTCACAGCAGAGCAACACATATGGGTCGCAACTAAAGCTGTTGAAATAAATGGTATGTGATTTCTTCTCAGGCCCAGACAATTTGATGAGCTACACGAACTTTATACTTATGACTTTGTTAAATGGATTAGTATTTCATAAATGTCGTCGTCTAGAATTACGTCAAAATATGGGAGAAATTTTATTAAAATGAGTAATGATTGTTACACCTATTTTCATTAGCAATAGTGGCACCTATTCATACTCTCCCCACATTAATTTACACACGAAGGAGAGATATAATCGTTGAAAAACAGGATGAAAACATAACAATCATCATAGATAGACGAGAGATGAATTTAATATTATATTCTTAATTAAAATACAGCAACCGCAGACGGGGAAGCATTCAACGTGTGCAACGGGTGGAGTTTCCGGTGGAAGGAGATATGGGGGGCGGTGGCGGAGAAACTTGGAGTGGCGACAACGGGCGCAGAGGAAGAGAAGGAGAGAATGTTTTCGAAGGAGTTTCACTATACGGCGGCAATGGGGGACAAAGGGAAGGTGTGGGCGGAGATAGTGGAGGAGGAAGGGTTGGTGACGACGGAAATGGAAGAGCTGGCGAATTGGGGGTTCTTAGACACATTGTTTAGGCTGCCGGAAAAAATGGTGGCGAGTCGAGCTAAGTCGGACCGATTTGGGTTCAATGTGAAATACAAGATGTTGGATTCTATGTTGTATTGGATTGATGTCATGAGAAATGACAAATTGATTCCTTCTTAA

mRNA sequence

ATGGCGGTCGAAATAATCAACACAAATTTGGATGCCAATGGCGAACTCGTCGCTGTCATATTTGGAGTCACCGGTTTGGTCGGAAAACAGTTGGCCAAAACCCTTCTCTCCACCGCCGGCTGGAAAGTCTACGGCGTCGCACGGCGGCCGGACAATGTCTCTCCCATCTCCCATCCCAAATTTCATTTCATCTCTTGTGATTTGCTCGACCGCCGGTCCGTTCAACAGAATCTCTCTCCCGTTCGCCACGTCACCCACCTTTTTTGGATCACTTGGGCAGCTCAGCTCCGGTTAGATTCCCCGGATTGCTCCGATCAGAATCGGGCCATGTTGGCCAACGCCCTCGACGCCATTCTCCCGTCCGCGCCGGCCCTCCGCCACGTCTCTCTACAGACCGGGATCAAACATTATGCTTCGTTGACGAGACTCGCCGCTGGCTGCGGCGGCGGAGGAGAAGGGGAGGTGGTTTATTGTGAGGATAGTCCGAGAGCGGAATCTGGGAACAATTTTTATTACGTGTTGGAAGATCTCCTCCGTGAGCGGTTGTCCTGTGGGCGGAGAATGGTGGCGTGGAGTGTTCTCCGACCCGGTTTGATATTGGGATGTTCGAACCGAACTTTCTTTAATTTTATGGGGAGTTTGTGTGTTTATGGTGCCATCTGTAAGAAGTTGAAGATGCCATTTGTGTTCGGCGGCACGGCGGCTTGCTGGGAGGAGGTTTATATCGACGGTTCCGATGCTCGACTCACAGCAGAGCAACACATATGGGTCGCAACTAAAGCTGTTGAAATAAATGCAACCGCAGACGGGGAAGCATTCAACGTGTGCAACGGGTGGAGTTTCCGGTGGAAGGAGATATGGGGGGCGGTGGCGGAGAAACTTGGAGTGGCGACAACGGGCGCAGAGGAAGAGAAGGAGAGAATGTTTTCGAAGGAGTTTCACTATACGGCGGCAATGGGGGACAAAGGGAAGGTGTGGGCGGAGATAGTGGAGGAGGAAGGGTTGGTGACGACGGAAATGGAAGAGCTGGCGAATTGGGGGTTCTTAGACACATTGTTTAGGCTGCCGGAAAAAATGGTGGCGAGTCGAGCTAAGTCGGACCGATTTGGGTTCAATGTGAAATACAAGATGTTGGATTCTATGTTGTATTGGATTGATGTCATGAGAAATGACAAATTGATTCCTTCTTAA

Coding sequence (CDS)

ATGGCGGTCGAAATAATCAACACAAATTTGGATGCCAATGGCGAACTCGTCGCTGTCATATTTGGAGTCACCGGTTTGGTCGGAAAACAGTTGGCCAAAACCCTTCTCTCCACCGCCGGCTGGAAAGTCTACGGCGTCGCACGGCGGCCGGACAATGTCTCTCCCATCTCCCATCCCAAATTTCATTTCATCTCTTGTGATTTGCTCGACCGCCGGTCCGTTCAACAGAATCTCTCTCCCGTTCGCCACGTCACCCACCTTTTTTGGATCACTTGGGCAGCTCAGCTCCGGTTAGATTCCCCGGATTGCTCCGATCAGAATCGGGCCATGTTGGCCAACGCCCTCGACGCCATTCTCCCGTCCGCGCCGGCCCTCCGCCACGTCTCTCTACAGACCGGGATCAAACATTATGCTTCGTTGACGAGACTCGCCGCTGGCTGCGGCGGCGGAGGAGAAGGGGAGGTGGTTTATTGTGAGGATAGTCCGAGAGCGGAATCTGGGAACAATTTTTATTACGTGTTGGAAGATCTCCTCCGTGAGCGGTTGTCCTGTGGGCGGAGAATGGTGGCGTGGAGTGTTCTCCGACCCGGTTTGATATTGGGATGTTCGAACCGAACTTTCTTTAATTTTATGGGGAGTTTGTGTGTTTATGGTGCCATCTGTAAGAAGTTGAAGATGCCATTTGTGTTCGGCGGCACGGCGGCTTGCTGGGAGGAGGTTTATATCGACGGTTCCGATGCTCGACTCACAGCAGAGCAACACATATGGGTCGCAACTAAAGCTGTTGAAATAAATGCAACCGCAGACGGGGAAGCATTCAACGTGTGCAACGGGTGGAGTTTCCGGTGGAAGGAGATATGGGGGGCGGTGGCGGAGAAACTTGGAGTGGCGACAACGGGCGCAGAGGAAGAGAAGGAGAGAATGTTTTCGAAGGAGTTTCACTATACGGCGGCAATGGGGGACAAAGGGAAGGTGTGGGCGGAGATAGTGGAGGAGGAAGGGTTGGTGACGACGGAAATGGAAGAGCTGGCGAATTGGGGGTTCTTAGACACATTGTTTAGGCTGCCGGAAAAAATGGTGGCGAGTCGAGCTAAGTCGGACCGATTTGGGTTCAATGTGAAATACAAGATGTTGGATTCTATGTTGTATTGGATTGATGTCATGAGAAATGACAAATTGATTCCTTCTTAA

Protein sequence

MAVEIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPKFHFISCDLLDRRSVQQNLSPVRHVTHLFWITWAAQLRLDSPDCSDQNRAMLANALDAILPSAPALRHVSLQTGIKHYASLTRLAAGCGGGGEGEVVYCEDSPRAESGNNFYYVLEDLLRERLSCGRRMVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDGSDARLTAEQHIWVATKAVEINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVATTGAEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMVASRAKSDRFGFNVKYKMLDSMLYWIDVMRNDKLIPS*
Homology
BLAST of CSPI01G23840 vs. ExPASy Swiss-Prot
Match: A0A1C9CX66 ((S)-8-oxocitronellyl enol synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC2 PE=1 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 7.7e-65
Identity = 157/383 (40.99%), Postives = 201/383 (52.48%), Query Frame = 0

Query: 17  VAVIFGVTGLVGKQLAKTL--LSTAG--WKVYGVARRPDNVSPISHPKFHFISCDLLDRR 76
           VA+I GVTG+VG  LA+ L    T G  WKVYGVARRP       HP   +I CD+ D  
Sbjct: 28  VALIIGVTGIVGNSLAEILPISDTPGGPWKVYGVARRPRPAWNADHP-VEYIQCDISDAS 87

Query: 77  SVQQNLSPVRHVTHLFWITWAAQLRLDSPDCSDQNRAMLANALDAILPSAPALRHVSLQT 136
                LSP+  VTH+FW+TWA   R    +C + N  ML N L+A++P A  L H+ LQT
Sbjct: 88  DTHTKLSPLTDVTHIFWVTWAN--RPTESECCELNGTMLRNVLNALIPKAANLHHICLQT 147

Query: 137 GIKHYASLTRLAAGCGGGGEGEVVYCEDSPRAESGNNFYYVLEDLLRERLSCGRRMVAWS 196
           G KHY          G     E  + ED PR  +  NFYY LED+L E  S  +  + WS
Sbjct: 148 GHKHYIGPFE---AFGKIKPHEPPFTEDMPRL-NAPNFYYTLEDMLVE-ASEKKAGLNWS 207

Query: 197 VLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDGSDARLTAE 256
           V RP +I G S  +  N +G+LCVY AICK    P  F GT A W   Y   SDA L AE
Sbjct: 208 VHRPAVIFGFSPFSMMNIIGTLCVYAAICKHENTPLKFPGTKAAW-NCYSVASDADLIAE 267

Query: 257 QHIWVATKAVEINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVATTGAEEEKERMFSKE 316
             IW A     ++  A  EAFN  NG  F+WK +W  +AE+ GV     +E +     K 
Sbjct: 268 HQIWAA-----VDPYAKNEAFNCSNGDLFKWKHLWKVLAEQFGVEYAEFDESE-----KP 327

Query: 317 FHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMVASRAKSDRFGFN 376
                 M DKG VW EIV E GL TT++E +A W F D +    E ++ S  KS   G+ 
Sbjct: 328 TSLVERMKDKGPVWEEIVRENGLHTTKLEGVATWWFADVILG-GECLLDSMNKSKEHGYL 387

Query: 377 VKYKMLDSMLYWIDVMRNDKLIP 396
                 +S++  ID M+  K++P
Sbjct: 388 GFRNTKNSLISVIDKMKAHKIVP 390

BLAST of CSPI01G23840 vs. ExPASy Swiss-Prot
Match: Q9STX2 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP1 PE=1 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 2.3e-64
Identity = 153/386 (39.64%), Postives = 206/386 (53.37%), Query Frame = 0

Query: 15  ELVAVIFGVTGLVGKQLAK--TLLSTAG--WKVYGVARRPDNVSPISHPKFHFISCDLLD 74
           E VA+I GVTG+VG  LA+   L  T G  WKVYGVARRP       HP   +I CD+ D
Sbjct: 26  ESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRPRPTWNADHP-IDYIQCDVSD 85

Query: 75  RRSVQQNLSPVRHVTHLFWITWAAQLRLDSPDCSDQNRAMLANALDAILPSAPALRHVSL 134
               +  LSP+  VTH+F++TW  +   +S +C + N +ML N L AI+P AP LRHV L
Sbjct: 86  AEDTRSKLSPLTDVTHVFYVTWTNR-ESESENC-EANGSMLRNVLQAIIPYAPNLRHVCL 145

Query: 135 QTGIKHY-ASLTRLAAGCGGGGEGEVVYCEDSPRAESGNNFYYVLEDLLRERLSCGRRMV 194
           QTG KHY    T +      G   +  + ED PR +   NFYY  ED+L E +      V
Sbjct: 146 QTGTKHYLGPFTNV-----DGPRHDPPFTEDMPRLQI-QNFYYTQEDILFEEIK-KIETV 205

Query: 195 AWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDGSDARL 254
            WS+ RP +I G S  +  N +G+LCVY AICK    P +F G+   WE  ++  SDA L
Sbjct: 206 TWSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLLFPGSKKAWEG-FMTASDADL 265

Query: 255 TAEQHIWVATKAVEINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVATTGAEEEKERMF 314
            AEQ IW A     ++  A  EAFN  N   F+WK +W  +AE+ G+   G EE      
Sbjct: 266 IAEQQIWAA-----VDPYAKNEAFNCNNADIFKWKHLWKILAEQFGIEEYGFEE------ 325

Query: 315 SKEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMVASRAKSDRF 374
            K       M  K +VW E+V+E  L   ++EE+  W F D +  + E M+ S  KS  +
Sbjct: 326 GKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGV-EGMIDSMNKSKEY 385

Query: 375 GFNVKYKMLDSMLYWIDVMRNDKLIP 396
           GF       +S + WID  +  K++P
Sbjct: 386 GFLGFRNSNNSFISWIDKYKAFKIVP 388

BLAST of CSPI01G23840 vs. ExPASy Swiss-Prot
Match: A0A221J5W8 ((S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta cataria OX=39347 GN=ISY1 PE=1 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 5.0e-64
Identity = 157/388 (40.46%), Postives = 211/388 (54.38%), Query Frame = 0

Query: 13  NGELVAVIFGVTGLVGKQLAK--TLLSTAG--WKVYGVARRPDNVSPISHPKFHFISCDL 72
           N E VA+I GVTG+VG  LA+   L  T G  WKVYGVARRP       HP   +ISCD+
Sbjct: 24  NYESVALIVGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRPRPSWNEDHP-ITYISCDV 83

Query: 73  LDRRSVQQNLSPVRHVTHLFWITWAAQLRLDSPDCSDQNRAMLANALDAILPSAPALRHV 132
           LD   V+  LSP+  VTH+F+ TW    R    +  + N  ML N L+A++P+ P L+H+
Sbjct: 84  LDSVDVEAKLSPLTDVTHIFYATWTK--RSTEKENCEANGKMLKNVLNAMIPNCPNLKHI 143

Query: 133 SLQTGIKHY-ASLTRLAAGCGGGGEGEVVYCEDSPRAESGNNFYYVLEDLLRERLSCGRR 192
            LQTG KHY  +   L + C      +    ED PR +S  N+YY  ED+L E +   + 
Sbjct: 144 CLQTGRKHYLGAFEDLKSKC-----HDPPLTEDLPRLDS-QNYYYTQEDILFEEVQ-KKE 203

Query: 193 MVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDGSDA 252
            + WSV RPG I G S  +  N +G+LCVY AICK       F G    W+  Y D SDA
Sbjct: 204 GLTWSVHRPGTIFGFSPYSMMNLVGTLCVYAAICKHEGAVLRFPGCKGAWDG-YSDCSDA 263

Query: 253 RLTAEQHIWVATKAVEINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVATTGAEEEKER 312
            L AE  IW A     ++  A  EAFNV NG  F+WK  W  +AE+ GV   G  EE ++
Sbjct: 264 DLIAEHQIWAA-----VDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFGV-ECGEYEEGQQ 323

Query: 313 MFSKEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMVASRAKSD 372
           +  ++      M DKG VW +IV E GL TT++E++ NW F D +    E  + +  KS 
Sbjct: 324 VKLQDL-----MKDKGPVWDKIVRENGLSTTKLEDVGNWWFSDIVLG-NECWLDTMNKSK 383

Query: 373 RFGFNVKYKMLDSMLYWIDVMRNDKLIP 396
             GF       +S + WID ++  K++P
Sbjct: 384 EHGFLGFRNSKNSFISWIDKVKAFKIVP 388

BLAST of CSPI01G23840 vs. ExPASy Swiss-Prot
Match: A0A221J5X3 ((S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta racemosa OX=54731 GN=ISY1 PE=1 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 2.5e-63
Identity = 156/387 (40.31%), Postives = 202/387 (52.20%), Query Frame = 0

Query: 13  NGELVAVIFGVTGLVGKQLAKTL----LSTAGWKVYGVARRPDNVSPISHPKFHFISCDL 72
           N E VA+I GVTG+VG  LA+ L         WKVYGVARRP       HP   +ISCD+
Sbjct: 24  NYESVALIVGVTGIVGNSLAEILPLSDTPCGPWKVYGVARRPRPSWNEDHP-ITYISCDV 83

Query: 73  LDRRSVQQNLSPVRHVTHLFWITWAAQLRLDSPDCSDQNRAMLANALDAILPSAPALRHV 132
           LD   V+  LSP+  VTH+F+ TW    R    +  + N  ML N L+A++P+ P L+H+
Sbjct: 84  LDSVDVEAKLSPLTDVTHIFYATWTK--RSTEKENCEANGKMLKNVLNAMIPNCPNLKHI 143

Query: 133 SLQTGIKHYASLTRLAAGCGGGGEGEVVYCEDSPRAESGNNFYYVLEDLLRERLSCGRRM 192
            LQTG KHY      A         +    ED PR +S  NFYY  ED+L E +   +  
Sbjct: 144 CLQTGRKHYLG----AFEDWKIKRHDPPLTEDLPRLDS-QNFYYTQEDILFEEVQ-KKES 203

Query: 193 VAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDGSDAR 252
           + WSV RPG I G S  +  N +G+LCVY AICK       F G    W+  Y D SDA 
Sbjct: 204 LTWSVHRPGTIFGFSPYSMMNLVGTLCVYAAICKHEGAVLRFPGCKGAWDG-YSDCSDAD 263

Query: 253 LTAEQHIWVATKAVEINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVATTGAEEEKERM 312
           L AE  IW A     ++  A  EAFNV NG  F+WK  W  +AE+ GV     EE     
Sbjct: 264 LIAEHQIWAA-----VDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFGVECGEYEE----- 323

Query: 313 FSKEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMVASRAKSDR 372
             KE      M DKG VW +IV E GL TT++E++ NW F D +    E  + +  KS  
Sbjct: 324 -GKEVKLQDLMKDKGPVWDKIVRENGLSTTKLEDVGNWWFSDIVLG-NECWLDTMNKSKE 383

Query: 373 FGFNVKYKMLDSMLYWIDVMRNDKLIP 396
            GF       +S + WID ++  K++P
Sbjct: 384 HGFLGFRNSKNSFISWIDKVKAFKIVP 388

BLAST of CSPI01G23840 vs. ExPASy Swiss-Prot
Match: A0A221J5X1 ((S)-8-oxocitronellyl enol synthase ISY2 OS=Nepeta cataria OX=39347 GN=ISY2 PE=1 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 2.5e-63
Identity = 150/386 (38.86%), Postives = 206/386 (53.37%), Query Frame = 0

Query: 15  ELVAVIFGVTGLVGKQLAK--TLLSTAG--WKVYGVARRPDNVSPISHPKFHFISCDLLD 74
           + VA+I GVTGLVG  LA+   L  T G  WKVYGVARRP       HP  ++ISCD+ +
Sbjct: 27  QCVALIVGVTGLVGNSLAEMLPLSDTPGGPWKVYGVARRPRPSWNEDHP-INYISCDVSN 86

Query: 75  RRSVQQNLSPVRHVTHLFWITWAAQLRLDSPDCSDQNRAMLANALDAILPSAPALRHVSL 134
              V+  L P+  VTH+F+ TW +  R    +  + N  ML N LD ++P+ P L+H+ L
Sbjct: 87  TAEVEAKLPPLSDVTHIFYATWTS--RSSEEENCEANGKMLKNVLDTMIPNCPNLKHICL 146

Query: 135 QTGIKHYASLTRLAAGCGGGGEGEVVYCEDSPRAESGNNFYYVLEDLLRERLSCGRRMVA 194
           QTG  HY +           G  +    ED PR  + NNFYY  ED+L E +   +  + 
Sbjct: 147 QTGRFHYVA---SVVDWKINGSHDTPLTEDLPRLNT-NNFYYTQEDILLEEVK-RKEGLT 206

Query: 195 WSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDGSDARLT 254
           WSV RPG I G S  +  N +G+LCVY AICK+      F G    W+  Y D +DA L 
Sbjct: 207 WSVHRPGTIFGFSPYSMMNLVGTLCVYAAICKQEGAVLRFPGCKGAWDG-YSDCADADLI 266

Query: 255 AEQHIWVATKAVEINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVATTGAEEEKERMFS 314
           AE +IW A     ++  A  ++FNV NG  F+WK +W  +AE+ GV   G E E+     
Sbjct: 267 AEHYIWAA-----LDPHAKNQSFNVSNGDVFKWKHLWKVLAEQFGVECGGYEYEE----G 326

Query: 315 KEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMVASRAKSDRFG 374
           ++      M DKG VW +IV E GL  T++E++  W F DT+    E  + S  KS   G
Sbjct: 327 QQVRLQDVMKDKGPVWDKIVRENGLSNTKLEDVGKWWFSDTIL-WNECRLDSMNKSKEHG 386

Query: 375 FNVKYKMLDSMLYWIDVMRNDKLIPS 397
           F       +  LYWI  ++  K++PS
Sbjct: 387 FLGFRNSKNCFLYWIHKLKAYKIVPS 393

BLAST of CSPI01G23840 vs. ExPASy TrEMBL
Match: A0A0A0LVI2 (Epimerase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G530150 PE=3 SV=1)

HSP 1 Score: 815.5 bits (2105), Expect = 1.0e-232
Identity = 395/396 (99.75%), Postives = 395/396 (99.75%), Query Frame = 0

Query: 1   MAVEIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60
           MAVEIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK
Sbjct: 1   MAVEIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60

Query: 61  FHFISCDLLDRRSVQQNLSPVRHVTHLFWITWAAQLRLDSPDCSDQNRAMLANALDAILP 120
           FHFISCDLLDRRSVQQNLSPVRHVTHLFWITWAAQLRLDSPDCSDQNRAMLANALDAILP
Sbjct: 61  FHFISCDLLDRRSVQQNLSPVRHVTHLFWITWAAQLRLDSPDCSDQNRAMLANALDAILP 120

Query: 121 SAPALRHVSLQTGIKHYASLTRLAAGCGGGGEGEVVYCEDSPRAESGNNFYYVLEDLLRE 180
           SAPALRHVSLQTGIKHYASLTRLAAGCGGGGEGEVVYCEDSPRAESGNNFYYVLEDLLRE
Sbjct: 121 SAPALRHVSLQTGIKHYASLTRLAAGCGGGGEGEVVYCEDSPRAESGNNFYYVLEDLLRE 180

Query: 181 RLSCGRRMVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240
           RLSCGRRMVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV
Sbjct: 181 RLSCGRRMVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240

Query: 241 YIDGSDARLTAEQHIWVATKAVEINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVATTG 300
           YIDGSDARLTAEQHIWVATKAVEINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVATTG
Sbjct: 241 YIDGSDARLTAEQHIWVATKAVEINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVATTG 300

Query: 301 AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMV 360
           AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMV
Sbjct: 301 AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMV 360

Query: 361 ASRAKSDRFGFNVKYKMLDSMLYWIDVMRNDKLIPS 397
           ASRAKSDR GFNVKYKMLDSMLYWIDVMRNDKLIPS
Sbjct: 361 ASRAKSDRLGFNVKYKMLDSMLYWIDVMRNDKLIPS 396

BLAST of CSPI01G23840 vs. ExPASy TrEMBL
Match: A0A5D3BGL7 (3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G001240 PE=3 SV=1)

HSP 1 Score: 776.2 bits (2003), Expect = 6.7e-221
Identity = 380/396 (95.96%), Postives = 385/396 (97.22%), Query Frame = 0

Query: 1   MAVEIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60
           MA +IINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK
Sbjct: 1   MAAQIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60

Query: 61  FHFISCDLLDRRSVQQNLSPVRHVTHLFWITWAAQLRLDSPDCSDQNRAMLANALDAILP 120
           FHFISCDLLDR+SVQQNLSPVRHVTHLFWITWAAQLRLDSP CSDQNRAMLANALDAILP
Sbjct: 61  FHFISCDLLDRQSVQQNLSPVRHVTHLFWITWAAQLRLDSPGCSDQNRAMLANALDAILP 120

Query: 121 SAPALRHVSLQTGIKHYASLTRLAAGCGGGGEGEVVYCEDSPRAESGNNFYYVLEDLLRE 180
           SAPALRHVSLQTG+KHYASLTRLAAGC  GG  EVVYCEDSPRAESGNNFYYVLEDLLRE
Sbjct: 121 SAPALRHVSLQTGVKHYASLTRLAAGC--GGREEVVYCEDSPRAESGNNFYYVLEDLLRE 180

Query: 181 RLSCGRRMVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240
           RLSCGRRMVAWSVLRPGLILG SNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV
Sbjct: 181 RLSCGRRMVAWSVLRPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240

Query: 241 YIDGSDARLTAEQHIWVATKAVEINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVATTG 300
           YIDGSDARLTAEQH+WVATKAVEINATADGEAFNVCNGWSFRWKEIWG VAEKLGVATT 
Sbjct: 241 YIDGSDARLTAEQHMWVATKAVEINATADGEAFNVCNGWSFRWKEIWGTVAEKLGVATT- 300

Query: 301 AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMV 360
           AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLV TEMEELANW FLDTLFRLPEKMV
Sbjct: 301 AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVATEMEELANWVFLDTLFRLPEKMV 360

Query: 361 ASRAKSDRFGFNVKYKMLDSMLYWIDVMRNDKLIPS 397
           ASRAKSDRFGFNVKYKMLDS+LYWIDVMRNDKLIPS
Sbjct: 361 ASRAKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS 393

BLAST of CSPI01G23840 vs. ExPASy TrEMBL
Match: A0A1S3C4Y3 (3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103497085 PE=3 SV=1)

HSP 1 Score: 776.2 bits (2003), Expect = 6.7e-221
Identity = 380/396 (95.96%), Postives = 385/396 (97.22%), Query Frame = 0

Query: 1   MAVEIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60
           MA +IINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK
Sbjct: 1   MAAQIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60

Query: 61  FHFISCDLLDRRSVQQNLSPVRHVTHLFWITWAAQLRLDSPDCSDQNRAMLANALDAILP 120
           FHFISCDLLDR+SVQQNLSPVRHVTHLFWITWAAQLRLDSP CSDQNRAMLANALDAILP
Sbjct: 61  FHFISCDLLDRQSVQQNLSPVRHVTHLFWITWAAQLRLDSPGCSDQNRAMLANALDAILP 120

Query: 121 SAPALRHVSLQTGIKHYASLTRLAAGCGGGGEGEVVYCEDSPRAESGNNFYYVLEDLLRE 180
           SAPALRHVSLQTG+KHYASLTRLAAGC  GG  EVVYCEDSPRAESGNNFYYVLEDLLRE
Sbjct: 121 SAPALRHVSLQTGVKHYASLTRLAAGC--GGREEVVYCEDSPRAESGNNFYYVLEDLLRE 180

Query: 181 RLSCGRRMVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240
           RLSCGRRMVAWSVLRPGLILG SNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV
Sbjct: 181 RLSCGRRMVAWSVLRPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240

Query: 241 YIDGSDARLTAEQHIWVATKAVEINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVATTG 300
           YIDGSDARLTAEQH+WVATKAVEINATADGEAFNVCNGWSFRWKEIWG VAEKLGVATT 
Sbjct: 241 YIDGSDARLTAEQHMWVATKAVEINATADGEAFNVCNGWSFRWKEIWGTVAEKLGVATT- 300

Query: 301 AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMV 360
           AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLV TEMEELANW FLDTLFRLPEKMV
Sbjct: 301 AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVATEMEELANWVFLDTLFRLPEKMV 360

Query: 361 ASRAKSDRFGFNVKYKMLDSMLYWIDVMRNDKLIPS 397
           ASRAKSDRFGFNVKYKMLDS+LYWIDVMRNDKLIPS
Sbjct: 361 ASRAKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS 393

BLAST of CSPI01G23840 vs. ExPASy TrEMBL
Match: A0A1S3C5H7 (3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X2 OS=Cucumis melo OX=3656 GN=LOC103497085 PE=3 SV=1)

HSP 1 Score: 765.8 bits (1976), Expect = 9.1e-218
Identity = 377/396 (95.20%), Postives = 382/396 (96.46%), Query Frame = 0

Query: 1   MAVEIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60
           MA +IINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK
Sbjct: 1   MAAQIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60

Query: 61  FHFISCDLLDRRSVQQNLSPVRHVTHLFWITWAAQLRLDSPDCSDQNRAMLANALDAILP 120
           FHFISCDLLDR+SVQQNLSPVRHVTHLFWITWAAQLRLDSP CSDQNRAMLANALDAILP
Sbjct: 61  FHFISCDLLDRQSVQQNLSPVRHVTHLFWITWAAQLRLDSPGCSDQNRAMLANALDAILP 120

Query: 121 SAPALRHVSLQTGIKHYASLTRLAAGCGGGGEGEVVYCEDSPRAESGNNFYYVLEDLLRE 180
           SAPALRHVSLQTG+KHYASLTRLAAGC  GG  EVVYCEDSPRAESGNNFYYVLEDLLRE
Sbjct: 121 SAPALRHVSLQTGVKHYASLTRLAAGC--GGREEVVYCEDSPRAESGNNFYYVLEDLLRE 180

Query: 181 RLSCGRRMVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240
           RLSCGRRMVAWSVLRPGLILG SNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV
Sbjct: 181 RLSCGRRMVAWSVLRPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240

Query: 241 YIDGSDARLTAEQHIWVATKAVEINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVATTG 300
           YIDGSDARLTAEQH+WVATKAVEIN   DGEAFNVCNGWSFRWKEIWG VAEKLGVATT 
Sbjct: 241 YIDGSDARLTAEQHMWVATKAVEIN---DGEAFNVCNGWSFRWKEIWGTVAEKLGVATT- 300

Query: 301 AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMV 360
           AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLV TEMEELANW FLDTLFRLPEKMV
Sbjct: 301 AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVATEMEELANWVFLDTLFRLPEKMV 360

Query: 361 ASRAKSDRFGFNVKYKMLDSMLYWIDVMRNDKLIPS 397
           ASRAKSDRFGFNVKYKMLDS+LYWIDVMRNDKLIPS
Sbjct: 361 ASRAKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS 390

BLAST of CSPI01G23840 vs. ExPASy TrEMBL
Match: A0A6J1I0N6 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111469437 PE=3 SV=1)

HSP 1 Score: 689.9 bits (1779), Expect = 6.3e-195
Identity = 340/396 (85.86%), Postives = 356/396 (89.90%), Query Frame = 0

Query: 1   MAVEIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60
           MA E +NTNLDANGELVAVIFGVTGLVGK+LAK LLSTAGWKVYGVARRPDN+SPIS P 
Sbjct: 1   MADESMNTNLDANGELVAVIFGVTGLVGKELAKMLLSTAGWKVYGVARRPDNISPISDPN 60

Query: 61  FHFISCDLLDRRSVQQNLSPVRHVTHLFWITWAAQLRLDSPDCSDQNRAMLANALDAILP 120
           FHFISCDLLDRRS   NLSPVRHVTH+FWITWAAQLR DS DCSDQN+AMLANALDAILP
Sbjct: 61  FHFISCDLLDRRSAHLNLSPVRHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILP 120

Query: 121 SAPALRHVSLQTGIKHYASLTRLAAGCGGGGEGEVVYCEDSPRAESGNNFYYVLEDLLRE 180
           SA ALRHVSLQTG+KHYASLT   +     G GEVVY EDSPRAESGNNFYYVLEDLLRE
Sbjct: 121 SASALRHVSLQTGVKHYASLTSSTS----SGGGEVVYSEDSPRAESGNNFYYVLEDLLRE 180

Query: 181 RLSCGRRMVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240
           RLS  RR+VAWS+LRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV
Sbjct: 181 RLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240

Query: 241 YIDGSDARLTAEQHIWVATKAVEINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVATTG 300
           YIDGSDARLTAEQH+W AT+AVEINA  D EAFNVCNGWSF WKEIWGAVAEKLGV T G
Sbjct: 241 YIDGSDARLTAEQHVWAATQAVEINAITDREAFNVCNGWSFTWKEIWGAVAEKLGV-TVG 300

Query: 301 AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMV 360
           AE+  ERMFS EFHYT AM +KG VWAEIVEEEGLV TEM ELANWGFLD+LFRLPEKMV
Sbjct: 301 AED--ERMFSPEFHYTEAMAEKGMVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMV 360

Query: 361 ASRAKSDRFGFNVKYKMLDSMLYWIDVMRNDKLIPS 397
           ASRAKSDR GF VKYKMLDS+LYWIDVMR+DKLIPS
Sbjct: 361 ASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIPS 389

BLAST of CSPI01G23840 vs. NCBI nr
Match: XP_004145319.1 (iridoid synthase CYC2 [Cucumis sativus] >KGN65803.1 hypothetical protein Csa_023251 [Cucumis sativus])

HSP 1 Score: 815.5 bits (2105), Expect = 2.1e-232
Identity = 395/396 (99.75%), Postives = 395/396 (99.75%), Query Frame = 0

Query: 1   MAVEIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60
           MAVEIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK
Sbjct: 1   MAVEIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60

Query: 61  FHFISCDLLDRRSVQQNLSPVRHVTHLFWITWAAQLRLDSPDCSDQNRAMLANALDAILP 120
           FHFISCDLLDRRSVQQNLSPVRHVTHLFWITWAAQLRLDSPDCSDQNRAMLANALDAILP
Sbjct: 61  FHFISCDLLDRRSVQQNLSPVRHVTHLFWITWAAQLRLDSPDCSDQNRAMLANALDAILP 120

Query: 121 SAPALRHVSLQTGIKHYASLTRLAAGCGGGGEGEVVYCEDSPRAESGNNFYYVLEDLLRE 180
           SAPALRHVSLQTGIKHYASLTRLAAGCGGGGEGEVVYCEDSPRAESGNNFYYVLEDLLRE
Sbjct: 121 SAPALRHVSLQTGIKHYASLTRLAAGCGGGGEGEVVYCEDSPRAESGNNFYYVLEDLLRE 180

Query: 181 RLSCGRRMVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240
           RLSCGRRMVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV
Sbjct: 181 RLSCGRRMVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240

Query: 241 YIDGSDARLTAEQHIWVATKAVEINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVATTG 300
           YIDGSDARLTAEQHIWVATKAVEINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVATTG
Sbjct: 241 YIDGSDARLTAEQHIWVATKAVEINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVATTG 300

Query: 301 AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMV 360
           AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMV
Sbjct: 301 AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMV 360

Query: 361 ASRAKSDRFGFNVKYKMLDSMLYWIDVMRNDKLIPS 397
           ASRAKSDR GFNVKYKMLDSMLYWIDVMRNDKLIPS
Sbjct: 361 ASRAKSDRLGFNVKYKMLDSMLYWIDVMRNDKLIPS 396

BLAST of CSPI01G23840 vs. NCBI nr
Match: XP_008457376.1 (PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X1 [Cucumis melo] >KAA0031816.1 3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X1 [Cucumis melo var. makuwa] >TYJ97318.1 3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 776.2 bits (2003), Expect = 1.4e-220
Identity = 380/396 (95.96%), Postives = 385/396 (97.22%), Query Frame = 0

Query: 1   MAVEIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60
           MA +IINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK
Sbjct: 1   MAAQIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60

Query: 61  FHFISCDLLDRRSVQQNLSPVRHVTHLFWITWAAQLRLDSPDCSDQNRAMLANALDAILP 120
           FHFISCDLLDR+SVQQNLSPVRHVTHLFWITWAAQLRLDSP CSDQNRAMLANALDAILP
Sbjct: 61  FHFISCDLLDRQSVQQNLSPVRHVTHLFWITWAAQLRLDSPGCSDQNRAMLANALDAILP 120

Query: 121 SAPALRHVSLQTGIKHYASLTRLAAGCGGGGEGEVVYCEDSPRAESGNNFYYVLEDLLRE 180
           SAPALRHVSLQTG+KHYASLTRLAAGC  GG  EVVYCEDSPRAESGNNFYYVLEDLLRE
Sbjct: 121 SAPALRHVSLQTGVKHYASLTRLAAGC--GGREEVVYCEDSPRAESGNNFYYVLEDLLRE 180

Query: 181 RLSCGRRMVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240
           RLSCGRRMVAWSVLRPGLILG SNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV
Sbjct: 181 RLSCGRRMVAWSVLRPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240

Query: 241 YIDGSDARLTAEQHIWVATKAVEINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVATTG 300
           YIDGSDARLTAEQH+WVATKAVEINATADGEAFNVCNGWSFRWKEIWG VAEKLGVATT 
Sbjct: 241 YIDGSDARLTAEQHMWVATKAVEINATADGEAFNVCNGWSFRWKEIWGTVAEKLGVATT- 300

Query: 301 AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMV 360
           AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLV TEMEELANW FLDTLFRLPEKMV
Sbjct: 301 AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVATEMEELANWVFLDTLFRLPEKMV 360

Query: 361 ASRAKSDRFGFNVKYKMLDSMLYWIDVMRNDKLIPS 397
           ASRAKSDRFGFNVKYKMLDS+LYWIDVMRNDKLIPS
Sbjct: 361 ASRAKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS 393

BLAST of CSPI01G23840 vs. NCBI nr
Match: XP_008457377.1 (PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X2 [Cucumis melo])

HSP 1 Score: 765.8 bits (1976), Expect = 1.9e-217
Identity = 377/396 (95.20%), Postives = 382/396 (96.46%), Query Frame = 0

Query: 1   MAVEIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60
           MA +IINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK
Sbjct: 1   MAAQIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60

Query: 61  FHFISCDLLDRRSVQQNLSPVRHVTHLFWITWAAQLRLDSPDCSDQNRAMLANALDAILP 120
           FHFISCDLLDR+SVQQNLSPVRHVTHLFWITWAAQLRLDSP CSDQNRAMLANALDAILP
Sbjct: 61  FHFISCDLLDRQSVQQNLSPVRHVTHLFWITWAAQLRLDSPGCSDQNRAMLANALDAILP 120

Query: 121 SAPALRHVSLQTGIKHYASLTRLAAGCGGGGEGEVVYCEDSPRAESGNNFYYVLEDLLRE 180
           SAPALRHVSLQTG+KHYASLTRLAAGC  GG  EVVYCEDSPRAESGNNFYYVLEDLLRE
Sbjct: 121 SAPALRHVSLQTGVKHYASLTRLAAGC--GGREEVVYCEDSPRAESGNNFYYVLEDLLRE 180

Query: 181 RLSCGRRMVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240
           RLSCGRRMVAWSVLRPGLILG SNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV
Sbjct: 181 RLSCGRRMVAWSVLRPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240

Query: 241 YIDGSDARLTAEQHIWVATKAVEINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVATTG 300
           YIDGSDARLTAEQH+WVATKAVEIN   DGEAFNVCNGWSFRWKEIWG VAEKLGVATT 
Sbjct: 241 YIDGSDARLTAEQHMWVATKAVEIN---DGEAFNVCNGWSFRWKEIWGTVAEKLGVATT- 300

Query: 301 AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMV 360
           AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLV TEMEELANW FLDTLFRLPEKMV
Sbjct: 301 AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVATEMEELANWVFLDTLFRLPEKMV 360

Query: 361 ASRAKSDRFGFNVKYKMLDSMLYWIDVMRNDKLIPS 397
           ASRAKSDRFGFNVKYKMLDS+LYWIDVMRNDKLIPS
Sbjct: 361 ASRAKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS 390

BLAST of CSPI01G23840 vs. NCBI nr
Match: XP_038895390.1 ((S)-8-oxocitronellyl enol synthase CYC2 [Benincasa hispida])

HSP 1 Score: 737.6 bits (1903), Expect = 5.5e-209
Identity = 362/397 (91.18%), Postives = 371/397 (93.45%), Query Frame = 0

Query: 1   MAVEIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60
           MA EIINTNL AN ELVAVIFGVTGLVGK+LAKTLLSTAGWKVYGVARRPDN+SPISHP 
Sbjct: 1   MAAEIINTNLVANAELVAVIFGVTGLVGKELAKTLLSTAGWKVYGVARRPDNISPISHPN 60

Query: 61  FHFISCDLLDRRSVQQNLSPVRHVTHLFWITWAAQLRLDSPDCSDQNRAMLANALDAILP 120
           FHFISCDLLDRRS   NLSPVRHVTHLFWITWAAQLR DS  CSDQNRAMLANALDAILP
Sbjct: 61  FHFISCDLLDRRSAHSNLSPVRHVTHLFWITWAAQLRFDSQGCSDQNRAMLANALDAILP 120

Query: 121 SAPALRHVSLQTGIKHYASLTRLAAGCGGGGEGEVVYCEDSPRAESGNNFYYVLEDLLRE 180
           SAPALRHVSLQTGIKHYASLTRLAAGCGGG  GEV YCEDSPRAESGNNFYYVLEDLLRE
Sbjct: 121 SAPALRHVSLQTGIKHYASLTRLAAGCGGG--GEVAYCEDSPRAESGNNFYYVLEDLLRE 180

Query: 181 RLSCGRRMVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240
           RLS GRR+V WSVLRPGLILG SNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV
Sbjct: 181 RLSGGRRVVGWSVLRPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240

Query: 241 YIDGSDARLTAEQHIWVATKAVEINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVATTG 300
           YIDGSDARLTAEQH+W ATK ++INATADGEAFNVCNGWSFRWKEIWGAVAEKLGVA   
Sbjct: 241 YIDGSDARLTAEQHVWAATKGIKINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVA--A 300

Query: 301 AEEEKER-MFSKEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKM 360
           AE+E ER MFS EFHYTAAMGDKGK+WAEIVEEEGLV TEMEELANWGFLDTLFRLPEKM
Sbjct: 301 AEKEDERIMFSAEFHYTAAMGDKGKLWAEIVEEEGLVPTEMEELANWGFLDTLFRLPEKM 360

Query: 361 VASRAKSDRFGFNVKYKMLDSMLYWIDVMRNDKLIPS 397
           VASRAKSDRFGF VKYKMLDS+LYWIDVMRNDKLIPS
Sbjct: 361 VASRAKSDRFGFTVKYKMLDSILYWIDVMRNDKLIPS 393

BLAST of CSPI01G23840 vs. NCBI nr
Match: KAG6583366.1 ((S)-8-oxocitronellyl enol synthase CYC2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 693.3 bits (1788), Expect = 1.2e-195
Identity = 341/396 (86.11%), Postives = 358/396 (90.40%), Query Frame = 0

Query: 1   MAVEIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60
           MA E +NTNLDANGELVAVIFGVTGLVGK+LAK LLSTAGWKVYGVARRPDN+SPIS P 
Sbjct: 1   MADESMNTNLDANGELVAVIFGVTGLVGKELAKLLLSTAGWKVYGVARRPDNISPISDPN 60

Query: 61  FHFISCDLLDRRSVQQNLSPVRHVTHLFWITWAAQLRLDSPDCSDQNRAMLANALDAILP 120
           FHFISCDLLDRRS   NLSP+ HVTH+FWITWAAQLR DS DCS+QN+AMLANALDAILP
Sbjct: 61  FHFISCDLLDRRSAHLNLSPLCHVTHMFWITWAAQLRFDSQDCSNQNKAMLANALDAILP 120

Query: 121 SAPALRHVSLQTGIKHYASLTRLAAGCGGGGEGEVVYCEDSPRAESGNNFYYVLEDLLRE 180
           SAPALRHVSLQTG+KHYASLT    G  GGG GEVVY EDSPRAESGNNFYYVLEDLLRE
Sbjct: 121 SAPALRHVSLQTGVKHYASLT----GSTGGGGGEVVYSEDSPRAESGNNFYYVLEDLLRE 180

Query: 181 RLSCGRRMVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240
           RLS  RR+VAWS+LRPGLILGCSNRTFFNFMGS+CVYGAICKKLKMPFVFGGT ACWEEV
Sbjct: 181 RLSGRRRVVAWSILRPGLILGCSNRTFFNFMGSVCVYGAICKKLKMPFVFGGTGACWEEV 240

Query: 241 YIDGSDARLTAEQHIWVATKAVEINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVATTG 300
           YIDGSDARLTAEQH+W ATKAVEINA  DGEAFNVCNGWSF WKEIWGAVAEKLGV T G
Sbjct: 241 YIDGSDARLTAEQHVWAATKAVEINAITDGEAFNVCNGWSFTWKEIWGAVAEKLGV-TAG 300

Query: 301 AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMV 360
           AE+  ERMFS EF YTAAM +KG VWAEIVEEEGLV TEM ELANWGFLD+LFRLPEKMV
Sbjct: 301 AED--ERMFSPEFRYTAAMAEKGTVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMV 360

Query: 361 ASRAKSDRFGFNVKYKMLDSMLYWIDVMRNDKLIPS 397
           ASRAKSDR GF VKYKMLDS+LYWIDVMR+DKLIPS
Sbjct: 361 ASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIPS 389

BLAST of CSPI01G23840 vs. TAIR 10
Match: AT5G58750.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 412.1 bits (1058), Expect = 4.9e-115
Identity = 210/383 (54.83%), Postives = 264/383 (68.93%), Query Frame = 0

Query: 15  ELVAVIFGVTGLVGKQLAKTLL-STAGWKVYGVARRPDNVSPISHPKFHFISCDLLDRRS 74
           E VA+IFGVTGLVG+++ KTLL S  GW++YGVAR P+  S      ++FISCDLL+   
Sbjct: 16  ENVALIFGVTGLVGREIVKTLLMSKPGWRIYGVARNPEINSMTK--MYNFISCDLLNASE 75

Query: 75  VQQNLSPVRH-VTHLFWITWAAQLRLDSPDCSDQNRAMLANALDAILPSAPALRHVSLQT 134
            +Q LSP++  V+H+FW+TW+ +  LD+ +C  QN+ ML NALDAILP+A  L+H SLQT
Sbjct: 76  TKQRLSPLQDIVSHVFWVTWSGEFPLDTDECCVQNKTMLMNALDAILPNAKRLKHFSLQT 135

Query: 135 GIKHYASLTRLAAGCGGGGEGEVVYCEDSPRAESGNNFYYVLEDLLRERLSCGRRMVAWS 194
           G+KHY SL        G G     Y E+ PR  SG NFYYVLEDLL+E+++  R  V WS
Sbjct: 136 GMKHYVSLVE-ETMARGEGSSLYYYSEECPRKSSGKNFYYVLEDLLKEKIT--RSSVVWS 195

Query: 195 VLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDGSDARLTAE 254
           V RPGL++G S+RT +NFMGSLCVYGA+CK L +PFVFGGT  CWEE YIDGSD+ L AE
Sbjct: 196 VQRPGLLMGSSSRTLYNFMGSLCVYGAMCKYLNLPFVFGGTRECWEESYIDGSDSNLVAE 255

Query: 255 QHIWVATKAVEINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVATTGAEEEKERMFSKE 314
           QHI+ AT          GEAFN  NG  F WKEIW  + +KLGV     +  +  MF + 
Sbjct: 256 QHIFAATSG---KVREKGEAFNAINGVGFTWKEIWPEIGKKLGV-----QVNETTMFDEG 315

Query: 315 FHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMVASRAKSDRFGFN 374
           F +   M ++  VW EIV +E LV TE+E+LANW FLD LFR P K++  R K DRFGF 
Sbjct: 316 FWFGREMVERKHVWDEIVVKEKLVRTEIEDLANWYFLDALFRCPFKLLGKREKVDRFGFK 375

Query: 375 VKYKMLDSMLYWIDVMRNDKLIP 396
            KY+ LDS+LYWIDVMR++KLIP
Sbjct: 376 RKYRTLDSVLYWIDVMRDEKLIP 385

BLAST of CSPI01G23840 vs. TAIR 10
Match: AT4G24220.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 247.7 bits (631), Expect = 1.6e-65
Identity = 153/386 (39.64%), Postives = 206/386 (53.37%), Query Frame = 0

Query: 15  ELVAVIFGVTGLVGKQLAK--TLLSTAG--WKVYGVARRPDNVSPISHPKFHFISCDLLD 74
           E VA+I GVTG+VG  LA+   L  T G  WKVYGVARRP       HP   +I CD+ D
Sbjct: 26  ESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRPRPTWNADHP-IDYIQCDVSD 85

Query: 75  RRSVQQNLSPVRHVTHLFWITWAAQLRLDSPDCSDQNRAMLANALDAILPSAPALRHVSL 134
               +  LSP+  VTH+F++TW  +   +S +C + N +ML N L AI+P AP LRHV L
Sbjct: 86  AEDTRSKLSPLTDVTHVFYVTWTNR-ESESENC-EANGSMLRNVLQAIIPYAPNLRHVCL 145

Query: 135 QTGIKHY-ASLTRLAAGCGGGGEGEVVYCEDSPRAESGNNFYYVLEDLLRERLSCGRRMV 194
           QTG KHY    T +      G   +  + ED PR +   NFYY  ED+L E +      V
Sbjct: 146 QTGTKHYLGPFTNV-----DGPRHDPPFTEDMPRLQI-QNFYYTQEDILFEEIK-KIETV 205

Query: 195 AWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDGSDARL 254
            WS+ RP +I G S  +  N +G+LCVY AICK    P +F G+   WE  ++  SDA L
Sbjct: 206 TWSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLLFPGSKKAWEG-FMTASDADL 265

Query: 255 TAEQHIWVATKAVEINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVATTGAEEEKERMF 314
            AEQ IW A     ++  A  EAFN  N   F+WK +W  +AE+ G+   G EE      
Sbjct: 266 IAEQQIWAA-----VDPYAKNEAFNCNNADIFKWKHLWKILAEQFGIEEYGFEE------ 325

Query: 315 SKEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMVASRAKSDRF 374
            K       M  K +VW E+V+E  L   ++EE+  W F D +  + E M+ S  KS  +
Sbjct: 326 GKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGV-EGMIDSMNKSKEY 385

Query: 375 GFNVKYKMLDSMLYWIDVMRNDKLIP 396
           GF       +S + WID  +  K++P
Sbjct: 386 GFLGFRNSNNSFISWIDKYKAFKIVP 388

BLAST of CSPI01G23840 vs. TAIR 10
Match: AT4G24220.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 247.7 bits (631), Expect = 1.6e-65
Identity = 153/386 (39.64%), Postives = 206/386 (53.37%), Query Frame = 0

Query: 15  ELVAVIFGVTGLVGKQLAK--TLLSTAG--WKVYGVARRPDNVSPISHPKFHFISCDLLD 74
           E VA+I GVTG+VG  LA+   L  T G  WKVYGVARRP       HP   +I CD+ D
Sbjct: 25  ESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRPRPTWNADHP-IDYIQCDVSD 84

Query: 75  RRSVQQNLSPVRHVTHLFWITWAAQLRLDSPDCSDQNRAMLANALDAILPSAPALRHVSL 134
               +  LSP+  VTH+F++TW  +   +S +C + N +ML N L AI+P AP LRHV L
Sbjct: 85  AEDTRSKLSPLTDVTHVFYVTWTNR-ESESENC-EANGSMLRNVLQAIIPYAPNLRHVCL 144

Query: 135 QTGIKHY-ASLTRLAAGCGGGGEGEVVYCEDSPRAESGNNFYYVLEDLLRERLSCGRRMV 194
           QTG KHY    T +      G   +  + ED PR +   NFYY  ED+L E +      V
Sbjct: 145 QTGTKHYLGPFTNV-----DGPRHDPPFTEDMPRLQI-QNFYYTQEDILFEEIK-KIETV 204

Query: 195 AWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDGSDARL 254
            WS+ RP +I G S  +  N +G+LCVY AICK    P +F G+   WE  ++  SDA L
Sbjct: 205 TWSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLLFPGSKKAWEG-FMTASDADL 264

Query: 255 TAEQHIWVATKAVEINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVATTGAEEEKERMF 314
            AEQ IW A     ++  A  EAFN  N   F+WK +W  +AE+ G+   G EE      
Sbjct: 265 IAEQQIWAA-----VDPYAKNEAFNCNNADIFKWKHLWKILAEQFGIEEYGFEE------ 324

Query: 315 SKEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMVASRAKSDRF 374
            K       M  K +VW E+V+E  L   ++EE+  W F D +  + E M+ S  KS  +
Sbjct: 325 GKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGV-EGMIDSMNKSKEY 384

Query: 375 GFNVKYKMLDSMLYWIDVMRNDKLIP 396
           GF       +S + WID  +  K++P
Sbjct: 385 GFLGFRNSNNSFISWIDKYKAFKIVP 387

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A1C9CX667.7e-6540.99(S)-8-oxocitronellyl enol synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC... [more]
Q9STX22.3e-6439.643-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP... [more]
A0A221J5W85.0e-6440.46(S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta cataria OX=39347 GN=ISY1 PE=1 ... [more]
A0A221J5X32.5e-6340.31(S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta racemosa OX=54731 GN=ISY1 PE=1... [more]
A0A221J5X12.5e-6338.86(S)-8-oxocitronellyl enol synthase ISY2 OS=Nepeta cataria OX=39347 GN=ISY2 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A0A0LVI21.0e-23299.75Epimerase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G530150 P... [more]
A0A5D3BGL76.7e-22195.963-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X1 OS=Cucumis melo var. makuwa... [more]
A0A1S3C4Y36.7e-22195.963-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X1 OS=Cucumis melo OX=3656 GN=... [more]
A0A1S3C5H79.1e-21895.203-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X2 OS=Cucumis melo OX=3656 GN=... [more]
A0A6J1I0N66.3e-19585.863-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
Match NameE-valueIdentityDescription
XP_004145319.12.1e-23299.75iridoid synthase CYC2 [Cucumis sativus] >KGN65803.1 hypothetical protein Csa_023... [more]
XP_008457376.11.4e-22095.96PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X1 [Cucumis melo] >... [more]
XP_008457377.11.9e-21795.20PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X2 [Cucumis melo][more]
XP_038895390.15.5e-20991.18(S)-8-oxocitronellyl enol synthase CYC2 [Benincasa hispida][more]
KAG6583366.11.2e-19586.11(S)-8-oxocitronellyl enol synthase CYC2, partial [Cucurbita argyrosperma subsp. ... [more]
Match NameE-valueIdentityDescription
AT5G58750.14.9e-11554.83NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G24220.11.6e-6539.64NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G24220.21.6e-6539.64NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.720coord: 11..395
e-value: 5.4E-100
score: 337.0
NoneNo IPR availablePANTHERPTHR324873-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASEcoord: 1..395
NoneNo IPR availablePANTHERPTHR32487:SF123-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASE ISOFORM X1coord: 1..395
NoneNo IPR availableCDDcd089485beta-POR_like_SDR_acoord: 17..334
e-value: 1.12141E-93
score: 281.442
IPR001509NAD-dependent epimerase/dehydratasePFAMPF01370Epimerasecoord: 19..87
e-value: 3.0E-7
score: 30.2
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 17..316

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G23840.1CSPI01G23840.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003824 catalytic activity