Bhi02G001729 (gene) Wax gourd (B227) v1

Overview
NameBhi02G001729
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionDNA-directed RNA polymerase subunit beta
Locationchr2: 56698135 .. 56698386 (+)
RNA-Seq ExpressionBhi02G001729
SyntenyBhi02G001729
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGTTTGGGCTCTAGAAGGATTTGGTGTTGCTCATATTTTACAAGAGATGCTTACTTATAAATCTGATCATATTAGAGCTCGGCAAGAAGTACTTGGTACTACAATCATTGGAGGAACAATACCTAAACCTGAGGATACTCCAGAATCTTTTCGATTGCTCGTTCGAGAATTACGATCTTTGGCTTTGGAACTGAATCATTTCCTTGTATCTGAGAAGAACATCCAGATTAATAGGAAGGAAGCTTAA

mRNA sequence

ATGGAGGTTTGGGCTCTAGAAGGATTTGGTGTTGCTCATATTTTACAAGAGATGCTTACTTATAAATCTGATCATATTAGAGCTCGGCAAGAAGTACTTGGTACTACAATCATTGGAGGAACAATACCTAAACCTGAGGATACTCCAGAATCTTTTCGATTGCTCGTTCGAGAATTACGATCTTTGGCTTTGGAACTGAATCATTTCCTTGTATCTGAGAAGAACATCCAGATTAATAGGAAGGAAGCTTAA

Coding sequence (CDS)

ATGGAGGTTTGGGCTCTAGAAGGATTTGGTGTTGCTCATATTTTACAAGAGATGCTTACTTATAAATCTGATCATATTAGAGCTCGGCAAGAAGTACTTGGTACTACAATCATTGGAGGAACAATACCTAAACCTGAGGATACTCCAGAATCTTTTCGATTGCTCGTTCGAGAATTACGATCTTTGGCTTTGGAACTGAATCATTTCCTTGTATCTGAGAAGAACATCCAGATTAATAGGAAGGAAGCTTAA

Protein sequence

MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELRSLALELNHFLVSEKNIQINRKEA
Homology
BLAST of Bhi02G001729 vs. TAIR 10
Match: ATCG00190.1 (RNA polymerase subunit beta )

HSP 1 Score: 162.2 bits (409), Expect = 1.8e-40
Identity = 80/82 (97.56%), Postives = 80/82 (97.56%), Query Frame = 0

Query: 1    MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELR 60
            MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPED PESFRLLVRELR
Sbjct: 990  MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDAPESFRLLVRELR 1049

Query: 61   SLALELNHFLVSEKNIQINRKE 83
            SLALELNHFLVSEKN QINRKE
Sbjct: 1050 SLALELNHFLVSEKNFQINRKE 1071

BLAST of Bhi02G001729 vs. ExPASy Swiss-Prot
Match: Q4VZP1 (DNA-directed RNA polymerase subunit beta OS=Cucumis sativus OX=3659 GN=rpoB PE=3 SV=2)

HSP 1 Score: 165.6 bits (418), Expect = 2.4e-40
Identity = 82/83 (98.80%), Postives = 82/83 (98.80%), Query Frame = 0

Query: 1    MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELR 60
            MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELR
Sbjct: 988  MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELR 1047

Query: 61   SLALELNHFLVSEKNIQINRKEA 84
            SLALELNHFLVSEKN QINRKEA
Sbjct: 1048 SLALELNHFLVSEKNFQINRKEA 1070

BLAST of Bhi02G001729 vs. ExPASy Swiss-Prot
Match: A0ZZ27 (DNA-directed RNA polymerase subunit beta OS=Gossypium barbadense OX=3634 GN=rpoB PE=3 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 8.9e-40
Identity = 81/83 (97.59%), Postives = 81/83 (97.59%), Query Frame = 0

Query: 1    MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELR 60
            MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPED PESFRLLVRELR
Sbjct: 988  MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDAPESFRLLVRELR 1047

Query: 61   SLALELNHFLVSEKNIQINRKEA 84
            SLALELNHFLVSEKN QINRKEA
Sbjct: 1048 SLALELNHFLVSEKNFQINRKEA 1070

BLAST of Bhi02G001729 vs. ExPASy Swiss-Prot
Match: Q2L8Z9 (DNA-directed RNA polymerase subunit beta OS=Gossypium hirsutum OX=3635 GN=rpoB PE=3 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 8.9e-40
Identity = 81/83 (97.59%), Postives = 81/83 (97.59%), Query Frame = 0

Query: 1    MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELR 60
            MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPED PESFRLLVRELR
Sbjct: 988  MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDAPESFRLLVRELR 1047

Query: 61   SLALELNHFLVSEKNIQINRKEA 84
            SLALELNHFLVSEKN QINRKEA
Sbjct: 1048 SLALELNHFLVSEKNFQINRKEA 1070

BLAST of Bhi02G001729 vs. ExPASy Swiss-Prot
Match: A4QK10 (DNA-directed RNA polymerase subunit beta OS=Arabis hirsuta OX=78191 GN=rpoB PE=3 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 2.6e-39
Identity = 80/82 (97.56%), Postives = 80/82 (97.56%), Query Frame = 0

Query: 1    MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELR 60
            MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPED PESFRLLVRELR
Sbjct: 990  MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDAPESFRLLVRELR 1049

Query: 61   SLALELNHFLVSEKNIQINRKE 83
            SLALELNHFLVSEKN QINRKE
Sbjct: 1050 SLALELNHFLVSEKNFQINRKE 1071

BLAST of Bhi02G001729 vs. ExPASy Swiss-Prot
Match: P50546 (DNA-directed RNA polymerase subunit beta OS=Arabidopsis thaliana OX=3702 GN=rpoB PE=3 SV=4)

HSP 1 Score: 162.2 bits (409), Expect = 2.6e-39
Identity = 80/82 (97.56%), Postives = 80/82 (97.56%), Query Frame = 0

Query: 1    MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELR 60
            MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPED PESFRLLVRELR
Sbjct: 990  MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDAPESFRLLVRELR 1049

Query: 61   SLALELNHFLVSEKNIQINRKE 83
            SLALELNHFLVSEKN QINRKE
Sbjct: 1050 SLALELNHFLVSEKNFQINRKE 1071

BLAST of Bhi02G001729 vs. NCBI nr
Match: AHM89856.1 (RNA polymerase beta subunit [Lagenaria siceraria])

HSP 1 Score: 165.6 bits (418), Expect = 1.8e-37
Identity = 82/83 (98.80%), Postives = 82/83 (98.80%), Query Frame = 0

Query: 1    MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELR 60
            MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELR
Sbjct: 988  MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELR 1047

Query: 61   SLALELNHFLVSEKNIQINRKEA 84
            SLALELNHFLVSEKN QINRKEA
Sbjct: 1048 SLALELNHFLVSEKNFQINRKEA 1070

BLAST of Bhi02G001729 vs. NCBI nr
Match: YP_009236272.1 (RNA polymerase beta subunit [Gynostemma pentaphyllum] >YP_009440149.1 RNA polymerase beta subunit [Gynostemma longipes] >AMF83983.1 RNA polymerase beta subunit [Gynostemma pentaphyllum] >ANI25177.1 RNA polymerase beta subunit [Gynostemma pentaphyllum] >ATG86892.1 RNA polymerase beta subunit [Gynostemma longipes])

HSP 1 Score: 165.6 bits (418), Expect = 1.8e-37
Identity = 82/83 (98.80%), Postives = 82/83 (98.80%), Query Frame = 0

Query: 1    MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELR 60
            MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELR
Sbjct: 988  MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELR 1047

Query: 61   SLALELNHFLVSEKNIQINRKEA 84
            SLALELNHFLVSEKN QINRKEA
Sbjct: 1048 SLALELNHFLVSEKNFQINRKEA 1070

BLAST of Bhi02G001729 vs. NCBI nr
Match: YP_009752827.1 (RNA polymerase subunit beta [Baijiania yunnanensis] >QIT05899.1 RNA polymerase subunit beta [Baijiania yunnanensis])

HSP 1 Score: 165.6 bits (418), Expect = 1.8e-37
Identity = 82/83 (98.80%), Postives = 82/83 (98.80%), Query Frame = 0

Query: 1    MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELR 60
            MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELR
Sbjct: 988  MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELR 1047

Query: 61   SLALELNHFLVSEKNIQINRKEA 84
            SLALELNHFLVSEKN QINRKEA
Sbjct: 1048 SLALELNHFLVSEKNFQINRKEA 1070

BLAST of Bhi02G001729 vs. NCBI nr
Match: QJR52948.1 (RNA polymerase beta subunit [Herpetospermum pedunculosum])

HSP 1 Score: 165.6 bits (418), Expect = 1.8e-37
Identity = 82/83 (98.80%), Postives = 82/83 (98.80%), Query Frame = 0

Query: 1    MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELR 60
            MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELR
Sbjct: 988  MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELR 1047

Query: 61   SLALELNHFLVSEKNIQINRKEA 84
            SLALELNHFLVSEKN QINRKEA
Sbjct: 1048 SLALELNHFLVSEKNFQINRKEA 1070

BLAST of Bhi02G001729 vs. NCBI nr
Match: YP_009753697.1 (RNA polymerase subunit beta [Trichosanthes nervifolia] >QIT05308.1 RNA polymerase subunit beta [Trichosanthes nervifolia])

HSP 1 Score: 165.6 bits (418), Expect = 1.8e-37
Identity = 82/83 (98.80%), Postives = 82/83 (98.80%), Query Frame = 0

Query: 1    MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELR 60
            MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELR
Sbjct: 988  MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELR 1047

Query: 61   SLALELNHFLVSEKNIQINRKEA 84
            SLALELNHFLVSEKN QINRKEA
Sbjct: 1048 SLALELNHFLVSEKNFQINRKEA 1070

BLAST of Bhi02G001729 vs. ExPASy TrEMBL
Match: A0A218KG28 (DNA-directed RNA polymerase subunit beta OS=Cucumis sativus var. hardwickii OX=319220 GN=rpoB PE=3 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 8.7e-38
Identity = 82/83 (98.80%), Postives = 82/83 (98.80%), Query Frame = 0

Query: 1    MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELR 60
            MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELR
Sbjct: 988  MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELR 1047

Query: 61   SLALELNHFLVSEKNIQINRKEA 84
            SLALELNHFLVSEKN QINRKEA
Sbjct: 1048 SLALELNHFLVSEKNFQINRKEA 1070

BLAST of Bhi02G001729 vs. ExPASy TrEMBL
Match: A0A1X9Q1B9 (DNA-directed RNA polymerase subunit beta OS=Cucumis sativus OX=3659 GN=rpoB PE=3 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 8.7e-38
Identity = 82/83 (98.80%), Postives = 82/83 (98.80%), Query Frame = 0

Query: 1    MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELR 60
            MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELR
Sbjct: 988  MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELR 1047

Query: 61   SLALELNHFLVSEKNIQINRKEA 84
            SLALELNHFLVSEKN QINRKEA
Sbjct: 1048 SLALELNHFLVSEKNFQINRKEA 1070

BLAST of Bhi02G001729 vs. ExPASy TrEMBL
Match: A0A249RZI0 (DNA-directed RNA polymerase subunit beta OS=Cucumis melo var. cantalupensis OX=3658 GN=rpoB PE=3 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 8.7e-38
Identity = 82/83 (98.80%), Postives = 82/83 (98.80%), Query Frame = 0

Query: 1    MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELR 60
            MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELR
Sbjct: 988  MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELR 1047

Query: 61   SLALELNHFLVSEKNIQINRKEA 84
            SLALELNHFLVSEKN QINRKEA
Sbjct: 1048 SLALELNHFLVSEKNFQINRKEA 1070

BLAST of Bhi02G001729 vs. ExPASy TrEMBL
Match: A0A1S4ETZ9 (DNA-directed RNA polymerase subunit beta OS=Cucumis melo OX=3656 GN=rpoB PE=3 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 8.7e-38
Identity = 82/83 (98.80%), Postives = 82/83 (98.80%), Query Frame = 0

Query: 1    MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELR 60
            MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELR
Sbjct: 988  MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELR 1047

Query: 61   SLALELNHFLVSEKNIQINRKEA 84
            SLALELNHFLVSEKN QINRKEA
Sbjct: 1048 SLALELNHFLVSEKNFQINRKEA 1070

BLAST of Bhi02G001729 vs. ExPASy TrEMBL
Match: A0A109WXU0 (DNA-directed RNA polymerase subunit beta OS=Gynostemma pentaphyllum OX=182084 GN=rpoB PE=3 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 8.7e-38
Identity = 82/83 (98.80%), Postives = 82/83 (98.80%), Query Frame = 0

Query: 1    MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELR 60
            MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELR
Sbjct: 988  MEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPKPEDTPESFRLLVRELR 1047

Query: 61   SLALELNHFLVSEKNIQINRKEA 84
            SLALELNHFLVSEKN QINRKEA
Sbjct: 1048 SLALELNHFLVSEKNFQINRKEA 1070

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
ATCG00190.11.8e-4097.56RNA polymerase subunit beta [more]
Match NameE-valueIdentityDescription
Q4VZP12.4e-4098.80DNA-directed RNA polymerase subunit beta OS=Cucumis sativus OX=3659 GN=rpoB PE=3... [more]
A0ZZ278.9e-4097.59DNA-directed RNA polymerase subunit beta OS=Gossypium barbadense OX=3634 GN=rpoB... [more]
Q2L8Z98.9e-4097.59DNA-directed RNA polymerase subunit beta OS=Gossypium hirsutum OX=3635 GN=rpoB P... [more]
A4QK102.6e-3997.56DNA-directed RNA polymerase subunit beta OS=Arabis hirsuta OX=78191 GN=rpoB PE=3... [more]
P505462.6e-3997.56DNA-directed RNA polymerase subunit beta OS=Arabidopsis thaliana OX=3702 GN=rpoB... [more]
Match NameE-valueIdentityDescription
AHM89856.11.8e-3798.80RNA polymerase beta subunit [Lagenaria siceraria][more]
YP_009236272.11.8e-3798.80RNA polymerase beta subunit [Gynostemma pentaphyllum] >YP_009440149.1 RNA polyme... [more]
YP_009752827.11.8e-3798.80RNA polymerase subunit beta [Baijiania yunnanensis] >QIT05899.1 RNA polymerase s... [more]
QJR52948.11.8e-3798.80RNA polymerase beta subunit [Herpetospermum pedunculosum][more]
YP_009753697.11.8e-3798.80RNA polymerase subunit beta [Trichosanthes nervifolia] >QIT05308.1 RNA polymeras... [more]
Match NameE-valueIdentityDescription
A0A218KG288.7e-3898.80DNA-directed RNA polymerase subunit beta OS=Cucumis sativus var. hardwickii OX=3... [more]
A0A1X9Q1B98.7e-3898.80DNA-directed RNA polymerase subunit beta OS=Cucumis sativus OX=3659 GN=rpoB PE=3... [more]
A0A249RZI08.7e-3898.80DNA-directed RNA polymerase subunit beta OS=Cucumis melo var. cantalupensis OX=3... [more]
A0A1S4ETZ98.7e-3898.80DNA-directed RNA polymerase subunit beta OS=Cucumis melo OX=3656 GN=rpoB PE=3 SV... [more]
A0A109WXU08.7e-3898.80DNA-directed RNA polymerase subunit beta OS=Gynostemma pentaphyllum OX=182084 GN... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007641RNA polymerase Rpb2, domain 7PFAMPF04560RNA_pol_Rpb2_7coord: 1..69
e-value: 5.1E-17
score: 62.0
IPR015712DNA-directed RNA polymerase, subunit 2PANTHERPTHR20856DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2coord: 1..75
NoneNo IPR availablePANTHERPTHR20856:SF30DNA-DIRECTED RNA POLYMERASE SUBUNIT BETAcoord: 1..75
NoneNo IPR availableSUPERFAMILY64484beta and beta-prime subunits of DNA dependent RNA-polymerasecoord: 1..76

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi02M001729Bhi02M001729mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006351 transcription, DNA-templated
cellular_component GO:0009507 chloroplast
molecular_function GO:0003677 DNA binding
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity
molecular_function GO:0032549 ribonucleoside binding