Lsi08G005460 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi08G005460
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Domain of unknown function (DUF303)
Location	chr08 : 13595065 .. 13596961 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACCGATTCAATTCCTAGTTCAAAAGCTACAACTTTTCCAAACAACATTTTTATTCTTGCAGGACAGAGTAACATGGCTGGCCGAGGTGGGGTCTCGCAAGACCCAACCACAGACAAAATGGTATGGGATGGATATATCCCGCCCGAATGTCAATCCAACGACTCGATCTTTCGATTGACAGCTGATTTGGTATGGGAACAAGCCCATGAACCACTACATTGGGATATTGATGTTGTAAAGACCAATGGGATTGGACCTGGCATGGCTTTTGCTAATGAGCTATTGGCTATAGCTGGGGAGAGCATTGGGACTATTGGTCTTGTTCCATGTGCTATTGGAGGATCTCATTTGAGAGAATGGGTTAAAGGGACTGATAGGTATACCAATTTGGTTGATAGGATTAAGGCTTCAGAAAAAAATGGAGGGAAAGTTCAAGGATTTTTATGGTATCAAGGAGAGTCTGATGCTGCTGTTGAAGAAGAAGCTATGTGTTATGAAAGAGAGCTCACTAAGTTATTCGTCGACTTGCGTGCGGACGTGAACCACCCGGACCTACCCTTTATCGTGGTTTGTCATCGTATTTTCATGCCTATAAATACTTTTTGAAAAACAATAAAAATATTTGAGTGCACCTGGACTGCAGCTCAAATTCCAATTATTCAATATGTCATGTAACAAATTTTTCTTTTTTAAAAATTTATTTTTTAAATAGCAATACTTGAGCGTATAAATCCTAAGATACACTTCTCTTTGTGCATCTTACTCTCATCACATACATAAAAATGTCAATTAAATATTTGAAGAAAAAAGTAAAAATAATTTGTCACGTGACAAACTATATGATTAGATTATTTGGTGAGATACACAAAGATATGTGTAACCTAGGAAGCACCTAAGTATTTTTCTTTTTTTTTTTAATATTTTTTTCATTGTGATTGAAGTGGATGCACATGAATATGATATAGCCTAGGTTGCATCCAAGTATTGTTAAAAAAAAGAAATACATTTTTGGTCAAAGTTTTGAGTTTAGCAAGATATTTTGAAAAAATATATTTTTTTCGTTTTCTCACTCTGTCTCTCCACGTGTCAGGCTCCTTCTCTGTTCATGTAGAACAAAGGGAGAAACTCTCTCTCTCCCCTCATTTCATATCATGGGAGAAGGAGGTGTTGGGCAGACAATAGGTAGAGTTGAGAGAGTGGGAAAAAGAAAAATACTTTTTATTTTTTAAAAAAAATTAAAAAATTATAGTGTGTTAGGGCACTGAGTTGAATTATGAAGTCTGTGTTTAGAATATAGAGTTGTAAGATAGAGTTTCTCAATAAATATGCATAATAAATATAGAGTTTGTTGATAAATGTGCAAACTTCAACCGTATTTAATACCAACTTACGTTGGTGAGCCAAATACCCTCTATATTTTTAATTTAAAAAGTGTCATTTTAGCTTATGTTAGTTACCGTAATGAAAAGCCGACAGCGTGAATCATACCAAACTAAAACCTCAGTGATTAAAATGTCAGCTCGAAAGTTCATCGAACAATAAAATGCTACCAAATCCAAACATTAAATACCAAAAGTGTATATATATATATATATATATATATATATATTTTTTTTTTTTTTGATTTCTTATTGTAACGCAGGTGAAGATAGTAACTCATGATTTTTTTATAAGTCCAAATATAAGCTTCAAGGAAGAAGTATGGAAGGCATTGGAGGCAGTGACACAGAAGCTACCAAACGTAAGAATGGTGGACGGTCCAATGGCAGTGGGCAACTTTGATGAAGGGCTTAACGAAGATAAAGGTCATCTCAACGTTAAATCTGAAGTCAAATTAGGCAAAATGTTTGCTCATTCCTTTTATTCAAACTTTGGTCACAACTTCATTTTCTAA

mRNA sequence

ATGACCGATTCAATTCCTAGTTCAAAAGCTACAACTTTTCCAAACAACATTTTTATTCTTGCAGGACAGAGTAACATGGCTGGCCGAGGTGGGGTCTCGCAAGACCCAACCACAGACAAAATGGTATGGGATGGATATATCCCGCCCGAATGTCAATCCAACGACTCGATCTTTCGATTGACAGCTGATTTGGTATGGGAACAAGCCCATGAACCACTACATTGGGATATTGATGTTGTAAAGACCAATGGGATTGGACCTGGCATGGCTTTTGCTAATGAGCTATTGGCTATAGCTGGGGAGAGCATTGGGACTATTGGTCTTGTTCCATGTGCTATTGGAGGATCTCATTTGAGAGAATGGGTTAAAGGGACTGATAGGTATACCAATTTGGTTGATAGGATTAAGGCTTCAGAAAAAAATGGAGGGAAAGTTCAAGGATTTTTATGGTATCAAGGAGAGTCTGATGCTGCTGTTGAAGAAGAAGCTATGTGTTATGAAAGAGAGCTCACTAAGTTATTCGTCGACTTGCGTGCGGACGTGAACCACCCGGACCTACCCTTTATCGTGGTGAAGATAGTAACTCATGATTTTTTTATAAGTCCAAATATAAGCTTCAAGGAAGAAGTATGGAAGGCATTGGAGGCAGTGACACAGAAGCTACCAAACGTAAGAATGGTGGACGGTCCAATGGCAGTGGGCAACTTTGATGAAGGGCTTAACGAAGATAAAGGTCATCTCAACGTTAAATCTGAAGTCAAATTAGGCAAAATGTTTGCTCATTCCTTTTATTCAAACTTTGGTCACAACTTCATTTTCTAA

Coding sequence (CDS)

ATGACCGATTCAATTCCTAGTTCAAAAGCTACAACTTTTCCAAACAACATTTTTATTCTTGCAGGACAGAGTAACATGGCTGGCCGAGGTGGGGTCTCGCAAGACCCAACCACAGACAAAATGGTATGGGATGGATATATCCCGCCCGAATGTCAATCCAACGACTCGATCTTTCGATTGACAGCTGATTTGGTATGGGAACAAGCCCATGAACCACTACATTGGGATATTGATGTTGTAAAGACCAATGGGATTGGACCTGGCATGGCTTTTGCTAATGAGCTATTGGCTATAGCTGGGGAGAGCATTGGGACTATTGGTCTTGTTCCATGTGCTATTGGAGGATCTCATTTGAGAGAATGGGTTAAAGGGACTGATAGGTATACCAATTTGGTTGATAGGATTAAGGCTTCAGAAAAAAATGGAGGGAAAGTTCAAGGATTTTTATGGTATCAAGGAGAGTCTGATGCTGCTGTTGAAGAAGAAGCTATGTGTTATGAAAGAGAGCTCACTAAGTTATTCGTCGACTTGCGTGCGGACGTGAACCACCCGGACCTACCCTTTATCGTGGTGAAGATAGTAACTCATGATTTTTTTATAAGTCCAAATATAAGCTTCAAGGAAGAAGTATGGAAGGCATTGGAGGCAGTGACACAGAAGCTACCAAACGTAAGAATGGTGGACGGTCCAATGGCAGTGGGCAACTTTGATGAAGGGCTTAACGAAGATAAAGGTCATCTCAACGTTAAATCTGAAGTCAAATTAGGCAAAATGTTTGCTCATTCCTTTTATTCAAACTTTGGTCACAACTTCATTTTCTAA

Protein sequence

MTDSIPSSKATTFPNNIFILAGQSNMAGRGGVSQDPTTDKMVWDGYIPPECQSNDSIFRLTADLVWEQAHEPLHWDIDVVKTNGIGPGMAFANELLAIAGESIGTIGLVPCAIGGSHLREWVKGTDRYTNLVDRIKASEKNGGKVQGFLWYQGESDAAVEEEAMCYERELTKLFVDLRADVNHPDLPFIVVKIVTHDFFISPNISFKEEVWKALEAVTQKLPNVRMVDGPMAVGNFDEGLNEDKGHLNVKSEVKLGKMFAHSFYSNFGHNFIF

Homology

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BLAST of Lsi08G005460 vs. Swiss-Prot
Match: CAES_ARATH (Probable carbohydrate esterase At4g34215 OS=Arabidopsis thaliana GN=At4g34215 PE=1 SV=2)

HSP 1 Score: 210.3 bits (534), Expect = 2.6e-53
Identity = 116/263 (44.11%), Postives = 151/263 (57.41%), Query Frame = 1


Query: 6   PSSKATTFPNNIFILAGQSNMAGRGGVSQDPTTDKMVWDGYIPPECQSNDSIFRLTADLV 65
           P  ++   PN IFIL+GQSNMAGRGGV +D   ++ VWD  +PPEC  N SI RL+ADL 
Sbjct: 13  PEIQSPIPPNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLR 72

Query: 66  WEQAHEPLHWDIDVVKTNGIGPGMAFANELLAIAGESIGTIGLVPCAIGGSHLREWVKGT 125
           WE+AHEPLH DID  K  G+GPGMAFAN +          IGLVPCA GG+ ++EW +G+
Sbjct: 73  WEEAHEPLHVDIDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWERGS 132

Query: 126 DRYTNLVDRIKASEKNGGKVQGFLWYQGESDAAVEEEAMCYERELTKLFVDLRADVNHPD 185
             Y  +V R + S K GG+++  LWYQGESD     +A  Y   + +L  +LR D+N P 
Sbjct: 133 HLYERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPS 192

Query: 186 LPFIVVKIVTHDFFISPNISFKEEVWKALEA-VTQKLPNVRMVDGPMAVGNFDEGLNEDK 245
           LP I V I +   +I           K  EA +  KL NV  VD           L  D 
Sbjct: 193 LPIIQVAIASGGGYID----------KVREAQLGLKLSNVVCVDAK------GLPLKSDN 252

Query: 246 GHLNVKSEVKLGKMFAHSFYSNF 268
            HL  +++V+LG   A ++ SNF
Sbjct: 253 LHLTTEAQVQLGLSLAQAYLSNF 259

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BLAST of Lsi08G005460 vs. TrEMBL
Match: A0A0A0K9J9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G058180 PE=4 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 8.4e-139
Identity = 237/272 (87.13%), Postives = 252/272 (92.65%), Query Frame = 1


Query: 1   MTDSIPSSKATTFPNNIFILAGQSNMAGRGGVSQDPTTDKMVWDGYIPPECQSNDSIFRL 60
           MTDSI S KATT PNNIFILAGQSNMAGRGGVS DPTTDKMVWDGYIP EC+SNDSIFRL
Sbjct: 1   MTDSILSPKATTSPNNIFILAGQSNMAGRGGVSLDPTTDKMVWDGYIPLECESNDSIFRL 60

Query: 61  TADLVWEQAHEPLHWDIDVVKTNGIGPGMAFANELLAIAGESIGTIGLVPCAIGGSHLRE 120
            AD+VWEQAHEPLHWDIDVVKTNGIGPGMAFANELLAI G+ IG IGLVPCAIGGSHL+E
Sbjct: 61  NADMVWEQAHEPLHWDIDVVKTNGIGPGMAFANELLAIGGKRIGAIGLVPCAIGGSHLKE 120

Query: 121 WVKGTDRYTNLVDRIKASEKNGGKVQGFLWYQGESDAAVEEEAMCYERELTKLFVDLRAD 180
           WVKGT+RY NLV+RI+ASEKNGG VQG LWYQGESDAAVEEEAMCYERELTK F+DLRAD
Sbjct: 121 WVKGTNRYDNLVERIRASEKNGGTVQGILWYQGESDAAVEEEAMCYERELTKFFIDLRAD 180

Query: 181 VNHPDLPFIVVKIVTHDFFISPNISFKEEVWKALEAVTQKLPNVRMVDGPMAVGNFDEGL 240
            NHP+LP I+VK+VTHDFF+SPNISFKEEV  ALEAVT +LPNV MVDGPMAVGNFD+GL
Sbjct: 181 TNHPELPIILVKLVTHDFFLSPNISFKEEVCNALEAVTHRLPNVTMVDGPMAVGNFDDGL 240

Query: 241 NEDKGHLNVKSEVKLGKMFAHSFYSNFGHNFI 273
           NEDKGHLNVKSEVKLGKMFAHSFYSNF HNF+
Sbjct: 241 NEDKGHLNVKSEVKLGKMFAHSFYSNFAHNFL 272

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BLAST of Lsi08G005460 vs. TrEMBL
Match: A0A0A0LNC5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G356040 PE=4 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 5.2e-64
Identity = 129/270 (47.78%), Postives = 170/270 (62.96%), Query Frame = 1


Query: 6   PSSKATTFPNNIFILAGQSNMAGRGGVSQDPTTDKMVWDGYIPPECQSNDSIFRLTADLV 65
           PS      P NIFILAGQSNMAGRGGV  +     + WDG +PPECQ   SI RL   L 
Sbjct: 17  PSLSGAASPKNIFILAGQSNMAGRGGVENN-AQGNLQWDGLVPPECQPQPSILRLNPGLQ 76

Query: 66  WEQAHEPLHWDIDVVKTNGIGPGMAFANELLAIAGESIGTIGLVPCAIGGSHLREWVKGT 125
           WE A EPLH  ID+ +T GIGPG+AFA+ELL  AG + G +GLVPCA GG+ + +W+K  
Sbjct: 77  WEIAREPLHLGIDIKRTPGIGPGIAFAHELLVKAGPNAGAVGLVPCARGGTLIEQWIKNP 136

Query: 126 DR-----YTNLVDRIKASEKNGGKVQGFLWYQGESDAAVEEEAMCYERELTKLFVDLRAD 185
                  Y N ++RIKAS+K+GG V+   W+QGESDAA+ + A+ Y+  L K F D+R D
Sbjct: 137 SNPSATFYQNFIERIKASDKDGGVVRALFWFQGESDAAMNDTAIRYKDNLKKFFTDIRDD 196

Query: 186 VNHPDLPFIVVKIVTHDFFISPNISFKEEVWKALEAVTQKLPNVRMVDGPMAVGNF--DE 245
           +    LP IVVKI  +DFF   +      V +A EAV+++LP+V  +D      N+  +E
Sbjct: 197 IKPRFLPIIVVKIALYDFFRQHDTHNLPAVREAKEAVSKELPDVVAIDSLKLPINYTTNE 256

Query: 246 GLNEDKGHLNVKSEVKLGKMFAHSFYSNFG 269
           G+N D GH N  +E+ LGK  A ++ S+FG
Sbjct: 257 GINLDHGHFNTTTEITLGKWLAETYLSHFG 285

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BLAST of Lsi08G005460 vs. TrEMBL
Match: E5GB85_CUCME (Putative uncharacterized protein OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 1.2e-63
Identity = 132/270 (48.89%), Postives = 171/270 (63.33%), Query Frame = 1


Query: 6   PSSKATTFPNNIFILAGQSNMAGRGGVSQDPTTDKMVWDGYIPPECQSNDSIFRLTADLV 65
           PS      P NIFIL GQSNMAGRGGV ++ ++ K  WDG IPP+C+ N SI RL A   
Sbjct: 20  PSLSGAVSPQNIFILGGQSNMAGRGGVEKN-SSGKFEWDGVIPPDCKPNPSILRLNAARQ 79

Query: 66  WEQAHEPLHWDIDVVKTNGIGPGMAFANELLAIAGESIGTIGLVPCAIGGSHLREWVK-- 125
           WE A EPLHWDIDV+K NGI PGM FA+ELL  AG   G +GLVP AIGG+ +R+W+K  
Sbjct: 80  WEVAREPLHWDIDVMKANGISPGMGFAHELLVKAGPRAGVVGLVPTAIGGTFIRQWLKND 139

Query: 126 ---GTDRYTNLVDRIKASEKNGGKVQGFLWYQGESDAAVEEEAMCYERELTKLFVDLRAD 185
                  Y NLV+RI+AS+K GG V+  LW+QGESDAAV+EEA+ Y+  L    +DLR D
Sbjct: 140 SYPNATYYQNLVERIQASDKEGGVVRALLWFQGESDAAVKEEAINYKDNLKTFIMDLRRD 199

Query: 186 VNHPDLPFIVVKIVTHDFFISPNISFKEEVWKALEAVTQKLPNVRMVDG---PMAVGNFD 245
           +    LP I+VKI  +DF  +        V  A EAV++++P+V ++D    PM +    
Sbjct: 200 IQPRFLPVIIVKIALYDFLRANATDNLSIVRAAQEAVSKEVPDVSIIDSWKLPMNL-KTR 259

Query: 246 EGLNEDKGHLNVKSEVKLGKMFAHSFYSNF 268
           EG N D+GH N   E+  GK  A ++ S +
Sbjct: 260 EGFNLDRGHFNTTIELTAGKWLADAYLSRY 287

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BLAST of Lsi08G005460 vs. TrEMBL
Match: A0A067KZS9_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_16089 PE=4 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 2.1e-57
Identity = 120/250 (48.00%), Postives = 157/250 (62.80%), Query Frame = 1


Query: 14  PNNIFILAGQSNMAGRGGVSQDPTTDKMVWDGYIPPECQSNDSIFRLTADLVWEQAHEPL 73
           P NIFILAGQSNMAGRGGV  D  T K  WDG +PP+CQ N SIFRL+ADL+W  AHEPL
Sbjct: 23  PTNIFILAGQSNMAGRGGVINDTKTGKTTWDGIVPPQCQPNPSIFRLSADLIWVPAHEPL 82

Query: 74  HWDIDVVKTNGIGPGMAFANELLAIAGESIGTIGLVPCAIGGSHLREWVKGTDRYTNLVD 133
           H DID  KTNG+GPGMAFAN +L     +IG +GLVPCAIGG+++ +W KG   Y  LV 
Sbjct: 83  HADIDYNKTNGVGPGMAFANAILT-EDPTIGVLGLVPCAIGGTNISQWAKGGFLYEQLVR 142

Query: 134 RIKASEKNGGKVQGFLWYQGESDAAVEEEAMCYERELTKLFVDLRADVNHPDLPFIVVKI 193
           R + + ++GG ++  LWYQGESD    E+A  Y+  L K F++LR+D+ +P LP I V +
Sbjct: 143 RTQVALRSGGALRAMLWYQGESDTINREDADSYKSRLEKFFLELRSDLQYPLLPIIQVAL 202

Query: 194 VTHDFFISPNISFKEEVWKALEAVTQKLPNVRMVDGPMAVGNFDEGLNEDKGHLNVKSEV 253
                  S    F E + +    +  KL NV+ VD           L  D+ HL   ++V
Sbjct: 203 A------SAEGPFTETIRETQLGI--KLANVKCVDAK------GLPLEPDRLHLTTPAQV 257

Query: 254 KLGKMFAHSF 264
           +LG+  A++F
Sbjct: 263 QLGQTLANAF 257

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BLAST of Lsi08G005460 vs. TrEMBL
Match: B9R763_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_1589130 PE=4 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 2.8e-57
Identity = 121/250 (48.40%), Postives = 151/250 (60.40%), Query Frame = 1


Query: 14  PNNIFILAGQSNMAGRGGVSQDPTTDKMVWDGYIPPECQSNDSIFRLTADLVWEQAHEPL 73
           P NIFILAGQSNMAGRGGV  D  T  + WDG +PP+CQ   S+FRL+ D  W  AHEPL
Sbjct: 23  PKNIFILAGQSNMAGRGGVVNDTKTGILRWDGIVPPQCQPEPSVFRLSGDFTWVLAHEPL 82

Query: 74  HWDIDVVKTNGIGPGMAFANELLAIAGESIGTIGLVPCAIGGSHLREWVKGTDRYTNLVD 133
           H DID  KTNGIGPGMAFAN +L     +IG +GLVPCAIGG+ + +W KG   Y  LV 
Sbjct: 83  HSDIDYNKTNGIGPGMAFANAVLT-KDPAIGVVGLVPCAIGGTAISQWEKGGFLYDQLVQ 142

Query: 134 RIKASEKNGGKVQGFLWYQGESDAAVEEEAMCYERELTKLFVDLRADVNHPDLPFIVVKI 193
           R + +  +GG ++  LWYQGESD  +EE+A  Y+  L K F D+RAD+ HP LP   V +
Sbjct: 143 RTRVALYSGGVLRAMLWYQGESDTLIEEDADSYKGRLEKFFTDVRADLQHPFLPIFQVAL 202

Query: 194 VTHDFFISPNISFKEEVWKALEAVTQKLPNVRMVDGPMAVGNFDEGLNEDKGHLNVKSEV 253
            + +    P I    E  K +     KLPNV  VD           L  D+ HL   ++V
Sbjct: 203 ASGE---GPVIDTIREAQKGI-----KLPNVHCVDAK------GLPLEPDRLHLTTPAQV 257

Query: 254 KLGKMFAHSF 264
            LG+M A +F
Sbjct: 263 HLGQMLADAF 257

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BLAST of Lsi08G005460 vs. TAIR10
Match: AT3G53010.1 (AT3G53010.1 Domain of unknown function (DUF303) )

HSP 1 Score: 212.6 bits (540), Expect = 3.0e-55
Identity = 124/264 (46.97%), Postives = 152/264 (57.58%), Query Frame = 1


Query: 4   SIPSSKATTFPNN--IFILAGQSNMAGRGGVSQDPTTDKMVWDGYIPPECQSNDSIFRLT 63
           S P  ++ T   N  IFILAGQSNMAGRGGV  D  T+  VWDG IPPEC+SN SI RLT
Sbjct: 16  SPPHLQSQTITRNISIFILAGQSNMAGRGGVYNDTATNTTVWDGVIPPECRSNPSILRLT 75

Query: 64  ADLVWEQAHEPLHWDIDVVKTNGIGPGMAFANELLAIAGESIGTIGLVPCAIGGSHLREW 123
           + L W++A EPLH DID+ KTNG+GPGM FAN ++       G +GLVPC+IGG+ L +W
Sbjct: 76  SKLEWKEAKEPLHVDIDINKTNGVGPGMPFANRVV----NRFGQVGLVPCSIGGTKLSQW 135

Query: 124 VKGTDRYTNLVDRIKA--SEKNGGKVQGFLWYQGESDAAVEEEAMCYERELTKLFVDLRA 183
            KG   Y   V R KA  +   GG  +  LWYQGESD     +A  Y++ L K F DLR 
Sbjct: 136 QKGEFLYEETVKRAKAAMASGGGGSYRAVLWYQGESDTVDMVDASVYKKRLVKFFSDLRN 195

Query: 184 DVNHPDLPFIVVKIVTHDFFISPNISFKEEVWKALEAVTQKLPNVRMVDGPMAVGNFDEG 243
           D+ HP+LP I V + T      P   + + V KA   +   L NV  VD           
Sbjct: 196 DLQHPNLPIIQVALATG---AGP---YLDAVRKA--QLKTDLENVYCVDAR------GLP 255

Query: 244 LNEDKGHLNVKSEVKLGKMFAHSF 264
           L  D  HL   S+V+LG M A SF
Sbjct: 256 LEPDGLHLTTSSQVQLGHMIAESF 261

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BLAST of Lsi08G005460 vs. TAIR10
Match: AT4G34215.1 (AT4G34215.1 Domain of unknown function (DUF303) )

HSP 1 Score: 210.3 bits (534), Expect = 1.5e-54
Identity = 116/263 (44.11%), Postives = 151/263 (57.41%), Query Frame = 1


Query: 6   PSSKATTFPNNIFILAGQSNMAGRGGVSQDPTTDKMVWDGYIPPECQSNDSIFRLTADLV 65
           P  ++   PN IFIL+GQSNMAGRGGV +D   ++ VWD  +PPEC  N SI RL+ADL 
Sbjct: 13  PEIQSPIPPNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLR 72

Query: 66  WEQAHEPLHWDIDVVKTNGIGPGMAFANELLAIAGESIGTIGLVPCAIGGSHLREWVKGT 125
           WE+AHEPLH DID  K  G+GPGMAFAN +          IGLVPCA GG+ ++EW +G+
Sbjct: 73  WEEAHEPLHVDIDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWERGS 132

Query: 126 DRYTNLVDRIKASEKNGGKVQGFLWYQGESDAAVEEEAMCYERELTKLFVDLRADVNHPD 185
             Y  +V R + S K GG+++  LWYQGESD     +A  Y   + +L  +LR D+N P 
Sbjct: 133 HLYERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPS 192

Query: 186 LPFIVVKIVTHDFFISPNISFKEEVWKALEA-VTQKLPNVRMVDGPMAVGNFDEGLNEDK 245
           LP I V I +   +I           K  EA +  KL NV  VD           L  D 
Sbjct: 193 LPIIQVAIASGGGYID----------KVREAQLGLKLSNVVCVDAK------GLPLKSDN 252

Query: 246 GHLNVKSEVKLGKMFAHSFYSNF 268
            HL  +++V+LG   A ++ SNF
Sbjct: 253 LHLTTEAQVQLGLSLAQAYLSNF 259

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BLAST of Lsi08G005460 vs. NCBI nr
Match: gi|449446514|ref|XP_004141016.1| (PREDICTED: probable carbohydrate esterase At4g34215 [Cucumis sativus])

HSP 1 Score: 501.1 bits (1289), Expect = 1.2e-138
Identity = 237/272 (87.13%), Postives = 252/272 (92.65%), Query Frame = 1


Query: 1   MTDSIPSSKATTFPNNIFILAGQSNMAGRGGVSQDPTTDKMVWDGYIPPECQSNDSIFRL 60
           MTDSI S KATT PNNIFILAGQSNMAGRGGVS DPTTDKMVWDGYIP EC+SNDSIFRL
Sbjct: 1   MTDSILSPKATTSPNNIFILAGQSNMAGRGGVSLDPTTDKMVWDGYIPLECESNDSIFRL 60

Query: 61  TADLVWEQAHEPLHWDIDVVKTNGIGPGMAFANELLAIAGESIGTIGLVPCAIGGSHLRE 120
            AD+VWEQAHEPLHWDIDVVKTNGIGPGMAFANELLAI G+ IG IGLVPCAIGGSHL+E
Sbjct: 61  NADMVWEQAHEPLHWDIDVVKTNGIGPGMAFANELLAIGGKRIGAIGLVPCAIGGSHLKE 120

Query: 121 WVKGTDRYTNLVDRIKASEKNGGKVQGFLWYQGESDAAVEEEAMCYERELTKLFVDLRAD 180
           WVKGT+RY NLV+RI+ASEKNGG VQG LWYQGESDAAVEEEAMCYERELTK F+DLRAD
Sbjct: 121 WVKGTNRYDNLVERIRASEKNGGTVQGILWYQGESDAAVEEEAMCYERELTKFFIDLRAD 180

Query: 181 VNHPDLPFIVVKIVTHDFFISPNISFKEEVWKALEAVTQKLPNVRMVDGPMAVGNFDEGL 240
            NHP+LP I+VK+VTHDFF+SPNISFKEEV  ALEAVT +LPNV MVDGPMAVGNFD+GL
Sbjct: 181 TNHPELPIILVKLVTHDFFLSPNISFKEEVCNALEAVTHRLPNVTMVDGPMAVGNFDDGL 240

Query: 241 NEDKGHLNVKSEVKLGKMFAHSFYSNFGHNFI 273
           NEDKGHLNVKSEVKLGKMFAHSFYSNF HNF+
Sbjct: 241 NEDKGHLNVKSEVKLGKMFAHSFYSNFAHNFL 272

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BLAST of Lsi08G005460 vs. NCBI nr
Match: gi|659082064|ref|XP_008441649.1| (PREDICTED: probable carbohydrate esterase At4g34215 [Cucumis melo])

HSP 1 Score: 466.8 bits (1200), Expect = 2.5e-128
Identity = 218/248 (87.90%), Postives = 232/248 (93.55%), Query Frame = 1


Query: 26  MAGRGGVSQDPTTDKMVWDGYIPPECQSNDSIFRLTADLVWEQAHEPLHWDIDVVKTNGI 85
           MAGRGGVSQDPTT+KMVWDGYIP ECQSNDSIFRL AD+VWE+AHEPLHWDIDVVKTNGI
Sbjct: 1   MAGRGGVSQDPTTEKMVWDGYIPLECQSNDSIFRLNADMVWEKAHEPLHWDIDVVKTNGI 60

Query: 86  GPGMAFANELLAIAGESIGTIGLVPCAIGGSHLREWVKGTDRYTNLVDRIKASEKNGGKV 145
           GPGM FANELLAI G+  G IGLVPCAIGGSHL+EWVKGTDRY NLV+RI+ASEK+GGKV
Sbjct: 61  GPGMPFANELLAIGGKRAGAIGLVPCAIGGSHLKEWVKGTDRYDNLVERIRASEKDGGKV 120

Query: 146 QGFLWYQGESDAAVEEEAMCYERELTKLFVDLRADVNHPDLPFIVVKIVTHDFFISPNIS 205
           +GFLWYQGESDAAVEEEAMCYERELTK F+DLRAD NHPDLP I+VKIVTHDFF+SPNIS
Sbjct: 121 KGFLWYQGESDAAVEEEAMCYERELTKFFIDLRADTNHPDLPIIMVKIVTHDFFLSPNIS 180

Query: 206 FKEEVWKALEAVTQKLPNVRMVDGPMAVGNFDEGLNEDKGHLNVKSEVKLGKMFAHSFYS 265
           FKEEV KALEAVT  LPNV MVDGPMAVGNFD+GLNEDKGHLNVKSEVKLGKMFAHSFYS
Sbjct: 181 FKEEVLKALEAVTHNLPNVTMVDGPMAVGNFDDGLNEDKGHLNVKSEVKLGKMFAHSFYS 240

Query: 266 NFGHNFIF 274
           NF HNF+F
Sbjct: 241 NFAHNFLF 248

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BLAST of Lsi08G005460 vs. NCBI nr
Match: gi|659087703|ref|XP_008444593.1| (PREDICTED: probable carbohydrate esterase At4g34215 [Cucumis melo])

HSP 1 Score: 266.2 bits (679), Expect = 6.5e-68
Identity = 138/271 (50.92%), Postives = 176/271 (64.94%), Query Frame = 1


Query: 6   PSSKATTFPNNIFILAGQSNMAGRGGVSQDPTTDKMVWDGYIPPECQSNDSIFRLTADLV 65
           PS      P NIFIL GQSNMAGRGGV ++ ++ K  WDG IPP+C+ N SI RL A   
Sbjct: 20  PSLSGAVSPQNIFILGGQSNMAGRGGVEKN-SSGKFEWDGVIPPDCKPNPSILRLNAARQ 79

Query: 66  WEQAHEPLHWDIDVVKTNGIGPGMAFANELLAIAGESIGTIGLVPCAIGGSHLREWVK-- 125
           WE A EPLHWDIDV+K NGI PGM FA+ELL  AG   G +GLVP AIGG+ +R+W+K  
Sbjct: 80  WEVAREPLHWDIDVMKANGISPGMGFAHELLVKAGPRAGVVGLVPTAIGGTFIRQWLKND 139

Query: 126 ---GTDRYTNLVDRIKASEKNGGKVQGFLWYQGESDAAVEEEAMCYERELTKLFVDLRAD 185
                  Y NLV+RI+AS+K GG V+  LW+QGESDAAV+EEA+ Y+  L    +DLR D
Sbjct: 140 SYPNATYYQNLVERIQASDKEGGVVRALLWFQGESDAAVKEEAINYKDNLKTFIMDLRRD 199

Query: 186 VNHPDLPFIVVKIVTHDFFISPNISFKEEVWKALEAVTQKLPNVRMVDGPMAVGNF--DE 245
           +    LP I+VKI  +DF  +        V  A EAV+++LP+V  +D      NF  +E
Sbjct: 200 IQPRFLPVIIVKIALYDFLRANATDNLSIVRAAQEAVSKELPDVVTIDALELPINFTTNE 259

Query: 246 GLNEDKGHLNVKSEVKLGKMFAHSFYSNFGH 270
           GLN D GH N  +E+ LGK  A+++ S+FGH
Sbjct: 260 GLNLDHGHFNTSTEITLGKWLANTYLSHFGH 289

[Close]

BLAST of Lsi08G005460 vs. NCBI nr
Match: gi|778671052|ref|XP_011649575.1| (PREDICTED: probable carbohydrate esterase At4g34215 [Cucumis sativus])

HSP 1 Score: 252.7 bits (644), Expect = 7.4e-64
Identity = 129/270 (47.78%), Postives = 170/270 (62.96%), Query Frame = 1


Query: 6   PSSKATTFPNNIFILAGQSNMAGRGGVSQDPTTDKMVWDGYIPPECQSNDSIFRLTADLV 65
           PS      P NIFILAGQSNMAGRGGV  +     + WDG +PPECQ   SI RL   L 
Sbjct: 17  PSLSGAASPKNIFILAGQSNMAGRGGVENN-AQGNLQWDGLVPPECQPQPSILRLNPGLQ 76

Query: 66  WEQAHEPLHWDIDVVKTNGIGPGMAFANELLAIAGESIGTIGLVPCAIGGSHLREWVKGT 125
           WE A EPLH  ID+ +T GIGPG+AFA+ELL  AG + G +GLVPCA GG+ + +W+K  
Sbjct: 77  WEIAREPLHLGIDIKRTPGIGPGIAFAHELLVKAGPNAGAVGLVPCARGGTLIEQWIKNP 136

Query: 126 DR-----YTNLVDRIKASEKNGGKVQGFLWYQGESDAAVEEEAMCYERELTKLFVDLRAD 185
                  Y N ++RIKAS+K+GG V+   W+QGESDAA+ + A+ Y+  L K F D+R D
Sbjct: 137 SNPSATFYQNFIERIKASDKDGGVVRALFWFQGESDAAMNDTAIRYKDNLKKFFTDIRDD 196

Query: 186 VNHPDLPFIVVKIVTHDFFISPNISFKEEVWKALEAVTQKLPNVRMVDGPMAVGNF--DE 245
           +    LP IVVKI  +DFF   +      V +A EAV+++LP+V  +D      N+  +E
Sbjct: 197 IKPRFLPIIVVKIALYDFFRQHDTHNLPAVREAKEAVSKELPDVVAIDSLKLPINYTTNE 256

Query: 246 GLNEDKGHLNVKSEVKLGKMFAHSFYSNFG 269
           G+N D GH N  +E+ LGK  A ++ S+FG
Sbjct: 257 GINLDHGHFNTTTEITLGKWLAETYLSHFG 285

[Close]

BLAST of Lsi08G005460 vs. NCBI nr
Match: gi|307135858|gb|ADN33727.1| (hypothetical protein [Cucumis melo subsp. melo])

HSP 1 Score: 251.5 bits (641), Expect = 1.7e-63
Identity = 132/270 (48.89%), Postives = 171/270 (63.33%), Query Frame = 1


Query: 6   PSSKATTFPNNIFILAGQSNMAGRGGVSQDPTTDKMVWDGYIPPECQSNDSIFRLTADLV 65
           PS      P NIFIL GQSNMAGRGGV ++ ++ K  WDG IPP+C+ N SI RL A   
Sbjct: 20  PSLSGAVSPQNIFILGGQSNMAGRGGVEKN-SSGKFEWDGVIPPDCKPNPSILRLNAARQ 79

Query: 66  WEQAHEPLHWDIDVVKTNGIGPGMAFANELLAIAGESIGTIGLVPCAIGGSHLREWVK-- 125
           WE A EPLHWDIDV+K NGI PGM FA+ELL  AG   G +GLVP AIGG+ +R+W+K  
Sbjct: 80  WEVAREPLHWDIDVMKANGISPGMGFAHELLVKAGPRAGVVGLVPTAIGGTFIRQWLKND 139

Query: 126 ---GTDRYTNLVDRIKASEKNGGKVQGFLWYQGESDAAVEEEAMCYERELTKLFVDLRAD 185
                  Y NLV+RI+AS+K GG V+  LW+QGESDAAV+EEA+ Y+  L    +DLR D
Sbjct: 140 SYPNATYYQNLVERIQASDKEGGVVRALLWFQGESDAAVKEEAINYKDNLKTFIMDLRRD 199

Query: 186 VNHPDLPFIVVKIVTHDFFISPNISFKEEVWKALEAVTQKLPNVRMVDG---PMAVGNFD 245
           +    LP I+VKI  +DF  +        V  A EAV++++P+V ++D    PM +    
Sbjct: 200 IQPRFLPVIIVKIALYDFLRANATDNLSIVRAAQEAVSKEVPDVSIIDSWKLPMNL-KTR 259

Query: 246 EGLNEDKGHLNVKSEVKLGKMFAHSFYSNF 268
           EG N D+GH N   E+  GK  A ++ S +
Sbjct: 260 EGFNLDRGHFNTTIELTAGKWLADAYLSRY 287

The following BLAST results are available for this feature:

BLAST of Lsi08G005460 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CAES_ARATH	2.6e-53	44.11	Probable carbohydrate esterase At4g34215 OS=Arabidopsis thaliana GN=At4g34215 PE...	[more]

BLAST of Lsi08G005460 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K9J9_CUCSA	8.4e-139	87.13	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G058180 PE=4 SV=1	[more]
A0A0A0LNC5_CUCSA	5.2e-64	47.78	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G356040 PE=4 SV=1	[more]
E5GB85_CUCME	1.2e-63	48.89	Putative uncharacterized protein OS=Cucumis melo subsp. melo PE=4 SV=1	[more]
A0A067KZS9_JATCU	2.1e-57	48.00	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_16089 PE=4 SV=1	[more]
B9R763_RICCO	2.8e-57	48.40	Putative uncharacterized protein OS=Ricinus communis GN=RCOM_1589130 PE=4 SV=1	[more]

BLAST of Lsi08G005460 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G53010.1	3.0e-55	46.97	Domain of unknown function (DUF303)	[more]
AT4G34215.1	1.5e-54	44.11	Domain of unknown function (DUF303)	[more]

BLAST of Lsi08G005460 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449446514\|ref\|XP_004141016.1\|	1.2e-138	87.13	PREDICTED: probable carbohydrate esterase At4g34215 [Cucumis sativus]	[more]
gi\|659082064\|ref\|XP_008441649.1\|	2.5e-128	87.90	PREDICTED: probable carbohydrate esterase At4g34215 [Cucumis melo]	[more]
gi\|659087703\|ref\|XP_008444593.1\|	6.5e-68	50.92	PREDICTED: probable carbohydrate esterase At4g34215 [Cucumis melo]	[more]
gi\|778671052\|ref\|XP_011649575.1\|	7.4e-64	47.78	PREDICTED: probable carbohydrate esterase At4g34215 [Cucumis sativus]	[more]
gi\|307135858\|gb\|ADN33727.1\|	1.7e-63	48.89	hypothetical protein [Cucumis melo subsp. melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR005181	SASA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G005460.1	Lsi08G005460.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005181	Sialate O-acetylesterase domain	PFAM	PF03629	SASA	coord: 144..227 score: 1.3
None	No IPR available	PANTHER	PTHR31988	FAMILY NOT NAMED	coord: 1..263 score: 1.2
None	No IPR available	PANTHER	PTHR31988:SF4	SUBFAMILY NOT NAMED	coord: 1..263 score: 1.2

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi08G005460	Csa6G057180	Cucumber (Chinese Long) v2	culsiB478
Lsi08G005460	CSPI06G05000	Wild cucumber (PI 183967)	cpilsiB487
Lsi08G005460	Cucsa.363600	Cucumber (Gy14) v1	cgylsiB588
Lsi08G005460	CmoCh15G006100	Cucurbita moschata (Rifu)	cmolsiB282
Lsi08G005460	Cla013828	Watermelon (97103) v1	lsiwmB466
Lsi08G005460	MELO3C008368	Melon (DHL92) v3.5.1	lsimeB475
Lsi08G005460	ClCG08G006890	Watermelon (Charleston Gray)	lsiwcgB439
Lsi08G005460	MELO3C008368.2	Melon (DHL92) v3.6.1	lsimedB494
Lsi08G005460	Cla97C08G149590	Watermelon (97103) v2	lsiwmbB445
Lsi08G005460	Bhi04G001375	Wax gourd	lsiwgoB602
Lsi08G005460	Cp4.1LG13g03940	Cucurbita pepo (Zucchini)	cpelsiB158
Lsi08G005460	CsGy6G004940	Cucumber (Gy14) v2	cgyblsiB444
Lsi08G005460	Carg01597	Silver-seed gourd	carlsiB758
Lsi08G005460	CsaV3_6G005360	Cucumber (Chinese Long) v3	cuclsiB509

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi08G005460	Bottle gourd (USVL1VR-Ls)	lsilsiB122
Lsi08G005460	Cucurbita maxima (Rimu)	cmalsiB294
Lsi08G005460	Cucurbita maxima (Rimu)	cmalsiB703
Lsi08G005460	Cucurbita moschata (Rifu)	cmolsiB697
Lsi08G005460	Cucurbita pepo (Zucchini)	cpelsiB382
Lsi08G005460	Melon (DHL92) v3.6.1	lsimedB487
Lsi08G005460	Silver-seed gourd	carlsiB059