CmoCh09G000750 (gene) Cucurbita moschata (Rifu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmoCh09G000750
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu))
Description	Alpha-amylase (3.2.1.1)
Location	Cmo_Chr09 : 355957 .. 358493 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGTTTTTCTGGTTCATGAAAGAATATATTGAAGCAGCGAAGTCCGTGTTTTCTATTGGAGAGTATTGGGATTCTTGTAATTATAATGGCCATGACTTGGACTACGATCAAGGTACGATGACGAATCTTTGTTCTTCGTATGTACTGTGTTGATAAATTGGAATCACGTTTTAAGCAATATCCCCTAACAGTTTCTTGGAATTTTCTAGTTTTTTAGTTTACTTGAATGGTTGGTGTTTGTGGATAATATCCTCTGTTGTTTTTCTCAATTCATATATTCCATAGTGATGATCACAACTCTAAATGCAGATAGACATAGAATGGATAATAGATTGGATTGACAACACCGGATAACTTTCATCTGCTTTTGATTTTACAACCAAGGGAGTTCTTCAGGTCTCCCTGTATGTGAGGCCATTGCAAACCCCCGCCCCCCCCAAAGTGTAATACTATGAACATCAAATCTTAATTATTTGAAATAGAAAGGATCGGGCGACAGTAATATCATGGATTTTCTATTCCCCTGACCTCAGAATGCTTCTTGCAGGTCCATTGGCCCCTTCCTGCCAGCCATATCATGGAGGCATCCAAACTGTATCAGCTTGGAATAGAGTGTTATCTTGTTTGCAGTCTAATTCTCGCTCACTCCGCTGTGGCTTTCTTTCAGCTATGCTTCGTTTTATAGTAAGTCTAAATATCTAATCACTTGTTCATCATAATTTGTTGTGGTTTTTGGATTGTTTGATCCAACAAAGCTTTTGTACGATTCGAGCCCCATCTTCCTATATTGGATGTTTTTAAGCAAGTTATTCTCTGAGATTTTACTACATAATAGGTTAGTTCCCAAAATTTAGCTGCAAATCTTAAGAGTAGAACTGTTTCGCAGGCTGAGTGATCAATCTTCGATGTTTCTAAACCATGGTAGAGGAGTACTTGTTGTCTTTTAATTGTTGCCTCAAAACCAGTTTTTACACCCCACCTGTTCCTTATCAGGTTGCAACTCCTATCATGGGTAGGACCTTCCCTTCAGTACGTATGAATTCTGATTTTTCACAAATTTTATGTTGTTTTCAAGATTATACTCCATAGACATGGATGTCTGTCTTTACATCTGGAGGTGTAATTAGTGAGCAATCATCGATTCTTAGGATGAATGTTTGGGCGTGGAGGAAGACTGAAGTCAATCTGAGTGTGTGAACCTCTGTGGATATTGCAAAGGGGTCTAGTGTATTAGAACTCTAAATTAAATTTGGTAAGATGTATATTGATTGGATGACTTCAGTTAAGGGATGAGATTTGAACATCCATTATGAAGTGCAGAACATAAGAGAAAGTAGGGAGAGAGTTTAGGCGTGCAAGCTGAAATATCAACCATGGACATGGTTTGATCGGTAGTTGATCGATTCAATTCATTTTGGATCAGTTTGATAGTTTGTTTAGGCATATATGATATTTCAAAAACGTTAGTTTCGGTATAATTTTGGCCATAAGTTGTGTCGAACGGACCTTATAGTGAGTGCCACATGGCCGAAGAGCAGGGAAGTGACTACCAAGCATCACCATCACAATACCATGGTCAATGGCTCTTATCATTTTTTGCTTTGAAACTCCAAAAGATATACAGACCAACGCCACCCTTCCACGGCTTCCCTTGCCCCAACCTTCATCGTGTATATGTTGTATTAGTTGGATCATTGCATTGTTCATCAACAGTGAACCTCTGTAGGTCTTATATTTTCTTTTTCTGAATTCCAGGAATAATCTAACCGTCCTGTGTGCACACTACTACCTTGTCCCAGTCATCAAACTTGGGCAACTTGGTTTTACCTGCCACTTGATTAATGGATAGACCGATTAATTTATTCTCGAGAGGTACTAATTAATCACAGTTAGCTAGTTTGCTTTTATAGATTTGACAAGTATATATATTAATGATTTATTAACTAGCACCACCACTAGCACATTCTATATGCCTGTTGGAAATTATGATTTTTTATTCCGGAAGACATTCAAACTGCTGTCCGAAGCCGCAGTCAGCAAAAAAAAGGGAAAAAAGGGAAAAAAGAAAAAAAAAGAAAAAAAAGAAGGGGTAGTTTGCCATTCATGTGACTGAATATTAGCTACCGGCGACAGTGCTGATTGCAGCAAATTGGTCTTCTTTTTTTTATATATATACCAAGCACTCATCTTTTTGTTCTCTAACTGCCTTTTCAGATTCTCTACATGCAGGATTGTAAAGTAGATTTGCTGAGGACTCCAAGTGCAGCAGCGATGAACACCTCTCTCTGACGTCCACCGCCGAACCATAATTGGCTTCTCAGGTACAGTTAAAAAACTGTTGATTTTTATTGACTTTGAGACATTTTCATTACGGCAGATATCCACTTTTTAACCACAACGGCTCGCATTCATTGGAAACAGTGTGGGAATCCCATCCAGCTGTGCGGTAAGCAAAAGTGTAAGGCCCACTTGAACAATAACAAAGTGTTGTTGGTTTTGCTGCTTGCTTGCTGCCTGTTTCTTTGAATGAAATTCTAC

mRNA sequence

GGTTTTTCTGGTTCATGAAAGAATATATTGAAGCAGCGAAGTCCGTGTTTTCTATTGGAGAGTATTGGGATTCTTGTAATTATAATGGCCATGACTTGGACTACGATCAAGGTCTCCCTGTATGTGAGGCCATTGCAAACCCCCGCCCCCCCCAAAGTGTCCATTGGCCCCTTCCTGCCAGCCATATCATGGAGGCATCCAAACTGTATCAGCTTGGAATAGAGTGTTATCTTGTTTGCAGTCTAATTCTCGCTCACTCCGCTGTGGCTTTCTTTCAGCTATGCTTCGTTTTATAGAATAATCTAACCGTCCTGTGTGCACACTACTACCTTGTCCCAGTCATCAAACTTGGGCAACTTGGTTTTACCTGCCACTTGATTAATGGATAGACCGATTAATTTATTCTCGAGAGATTCTCTACATGCAGGATTGTAAAGTAGATTTGCTGAGGACTCCAAGTGCAGCAGCGATGAACACCTCTCTCTGACGTCCACCGCCGAACCATAATTGGCTTCTCAGTGTGGGAATCCCATCCAGCTGTGCGGTAAGCAAAAGTGTAAGGCCCACTTGAACAATAACAAAGTGTTGTTGGTTTTGCTGCTTGCTTGCTGCCTGTTTCTTTGAATGAAATTCTAC

Coding sequence (CDS)

ATGAAAGAATATATTGAAGCAGCGAAGTCCGTGTTTTCTATTGGAGAGTATTGGGATTCTTGTAATTATAATGGCCATGACTTGGACTACGATCAAGGTCTCCCTGTATGTGAGGCCATTGCAAACCCCCGCCCCCCCCAAAGTGTCCATTGGCCCCTTCCTGCCAGCCATATCATGGAGGCATCCAAACTGTATCAGCTTGGAATAGAGTGTTATCTTGTTTGCAGTCTAATTCTCGCTCACTCCGCTGTGGCTTTCTTTCAGCTATGCTTCGTTTTATAG

Homology

[Close]

BLAST of CmoCh09G000750 vs. Swiss-Prot
Match: AMY2_ARATH (Probable alpha-amylase 2 OS=Arabidopsis thaliana GN=AMY2 PE=2 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 2.6e-08
Identity = 24/32 (75.00%), Postives = 28/32 (87.50%), Query Frame = 1


Query: 1   MKEYIEAAKSVFSIGEYWDSCNYNGHDLDYDQ 33
           +KEYI AAK +FS+GE WDSCNYNGH LDY+Q
Sbjct: 203 VKEYIGAAKPLFSVGECWDSCNYNGHGLDYNQ 234

[Close]

BLAST of CmoCh09G000750 vs. TrEMBL
Match: E5GCD3_CUCME (Alpha-amylase OS=Cucumis melo subsp. melo PE=3 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 1.6e-09
Identity = 29/34 (85.29%), Postives = 32/34 (94.12%), Query Frame = 1


Query: 1   MKEYIEAAKSVFSIGEYWDSCNYNGHDLDYDQGL 35
           +KEYIEAAK +FSIGEYWDSCNYNGHDLDY+Q L
Sbjct: 203 VKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQAL 236

[Close]

BLAST of CmoCh09G000750 vs. TrEMBL
Match: A0A0D2UUL0_GOSRA (Alpha-amylase OS=Gossypium raimondii GN=B456_009G268400 PE=3 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 6.8e-08
Identity = 25/32 (78.12%), Postives = 29/32 (90.62%), Query Frame = 1


Query: 1   MKEYIEAAKSVFSIGEYWDSCNYNGHDLDYDQ 33
           +KEYIE AK +FS+GEYWDSCNYNGH LDY+Q
Sbjct: 199 VKEYIEGAKPIFSVGEYWDSCNYNGHGLDYNQ 230

[Close]

BLAST of CmoCh09G000750 vs. TrEMBL
Match: A0A0D2TSE8_GOSRA (Alpha-amylase OS=Gossypium raimondii GN=B456_009G268400 PE=3 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 6.8e-08
Identity = 25/32 (78.12%), Postives = 29/32 (90.62%), Query Frame = 1


Query: 1   MKEYIEAAKSVFSIGEYWDSCNYNGHDLDYDQ 33
           +KEYIE AK +FS+GEYWDSCNYNGH LDY+Q
Sbjct: 199 VKEYIEGAKPIFSVGEYWDSCNYNGHGLDYNQ 230

[Close]

BLAST of CmoCh09G000750 vs. TrEMBL
Match: A0A0A0L5R6_CUCSA (Alpha-amylase OS=Cucumis sativus GN=Csa_4G652880 PE=3 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 6.8e-08
Identity = 26/32 (81.25%), Postives = 29/32 (90.62%), Query Frame = 1


Query: 1   MKEYIEAAKSVFSIGEYWDSCNYNGHDLDYDQ 33
           +KEYIEA K + SIGEYWDSCNYNGHDLDY+Q
Sbjct: 203 VKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQ 234

[Close]

BLAST of CmoCh09G000750 vs. TrEMBL
Match: M5XLS5_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa006402mg PE=4 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 6.8e-08
Identity = 25/32 (78.12%), Postives = 29/32 (90.62%), Query Frame = 1


Query: 1   MKEYIEAAKSVFSIGEYWDSCNYNGHDLDYDQ 33
           +KEYIE AK +FS+GEYWDSCNYNGH LDY+Q
Sbjct: 203 VKEYIEGAKPIFSVGEYWDSCNYNGHGLDYNQ 234

[Close]

BLAST of CmoCh09G000750 vs. TAIR10
Match: AT1G76130.1 (AT1G76130.1 alpha-amylase-like 2)

HSP 1 Score: 59.3 bits (142), Expect = 1.4e-09
Identity = 24/32 (75.00%), Postives = 28/32 (87.50%), Query Frame = 1


Query: 1   MKEYIEAAKSVFSIGEYWDSCNYNGHDLDYDQ 33
           +KEYI AAK +FS+GE WDSCNYNGH LDY+Q
Sbjct: 203 VKEYIGAAKPLFSVGECWDSCNYNGHGLDYNQ 234

[Close]

BLAST of CmoCh09G000750 vs. NCBI nr
Match: gi|307136308|gb|ADN34132.1| (cytosolic alpha-amylase [Cucumis melo subsp. melo])

HSP 1 Score: 70.1 bits (170), Expect = 2.3e-09
Identity = 29/34 (85.29%), Postives = 32/34 (94.12%), Query Frame = 1


Query: 1   MKEYIEAAKSVFSIGEYWDSCNYNGHDLDYDQGL 35
           +KEYIEAAK +FSIGEYWDSCNYNGHDLDY+Q L
Sbjct: 203 VKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQAL 236

[Close]

BLAST of CmoCh09G000750 vs. NCBI nr
Match: gi|659104363|ref|XP_008452918.1| (PREDICTED: probable alpha-amylase 2 isoform X2 [Cucumis melo])

HSP 1 Score: 68.6 bits (166), Expect = 6.8e-09
Identity = 28/32 (87.50%), Postives = 31/32 (96.88%), Query Frame = 1


Query: 1   MKEYIEAAKSVFSIGEYWDSCNYNGHDLDYDQ 33
           +KEYIEAAK +FSIGEYWDSCNYNGHDLDY+Q
Sbjct: 203 VKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQ 234

[Close]

BLAST of CmoCh09G000750 vs. NCBI nr
Match: gi|659104355|ref|XP_008452914.1| (PREDICTED: probable alpha-amylase 2 isoform X1 [Cucumis melo])

HSP 1 Score: 68.6 bits (166), Expect = 6.8e-09
Identity = 28/32 (87.50%), Postives = 31/32 (96.88%), Query Frame = 1


Query: 1   MKEYIEAAKSVFSIGEYWDSCNYNGHDLDYDQ 33
           +KEYIEAAK +FSIGEYWDSCNYNGHDLDY+Q
Sbjct: 228 VKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQ 259

[Close]

BLAST of CmoCh09G000750 vs. NCBI nr
Match: gi|659104371|ref|XP_008452921.1| (PREDICTED: probable alpha-amylase 2 isoform X3 [Cucumis melo])

HSP 1 Score: 68.6 bits (166), Expect = 6.8e-09
Identity = 28/32 (87.50%), Postives = 31/32 (96.88%), Query Frame = 1


Query: 1   MKEYIEAAKSVFSIGEYWDSCNYNGHDLDYDQ 33
           +KEYIEAAK +FSIGEYWDSCNYNGHDLDY+Q
Sbjct: 228 VKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQ 259

[Close]

BLAST of CmoCh09G000750 vs. NCBI nr
Match: gi|645228846|ref|XP_008221185.1| (PREDICTED: probable alpha-amylase 2 [Prunus mume])

HSP 1 Score: 64.7 bits (156), Expect = 9.8e-08
Identity = 25/32 (78.12%), Postives = 29/32 (90.62%), Query Frame = 1


Query: 1   MKEYIEAAKSVFSIGEYWDSCNYNGHDLDYDQ 33
           +KEYIE AK +FS+GEYWDSCNYNGH LDY+Q
Sbjct: 203 VKEYIEGAKPIFSVGEYWDSCNYNGHGLDYNQ 234

The following BLAST results are available for this feature:

BLAST of CmoCh09G000750 vs. Swiss-Prot


Match Name	E-value	Identity	Description
AMY2_ARATH	2.6e-08	75.00	Probable alpha-amylase 2 OS=Arabidopsis thaliana GN=AMY2 PE=2 SV=1	[more]

BLAST of CmoCh09G000750 vs. TrEMBL


Match Name	E-value	Identity	Description
E5GCD3_CUCME	1.6e-09	85.29	Alpha-amylase OS=Cucumis melo subsp. melo PE=3 SV=1	[more]
A0A0D2UUL0_GOSRA	6.8e-08	78.13	Alpha-amylase OS=Gossypium raimondii GN=B456_009G268400 PE=3 SV=1	[more]
A0A0D2TSE8_GOSRA	6.8e-08	78.13	Alpha-amylase OS=Gossypium raimondii GN=B456_009G268400 PE=3 SV=1	[more]
A0A0A0L5R6_CUCSA	6.8e-08	81.25	Alpha-amylase OS=Cucumis sativus GN=Csa_4G652880 PE=3 SV=1	[more]
M5XLS5_PRUPE	6.8e-08	78.13	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa006402mg PE=4 SV=1	[more]

BLAST of CmoCh09G000750 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G76130.1	1.4e-09	75.00	alpha-amylase-like 2	[more]

BLAST of CmoCh09G000750 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|307136308\|gb\|ADN34132.1\|	2.3e-09	85.29	cytosolic alpha-amylase [Cucumis melo subsp. melo]	[more]
gi\|659104363\|ref\|XP_008452918.1\|	6.8e-09	87.50	PREDICTED: probable alpha-amylase 2 isoform X2 [Cucumis melo]	[more]
gi\|659104355\|ref\|XP_008452914.1\|	6.8e-09	87.50	PREDICTED: probable alpha-amylase 2 isoform X1 [Cucumis melo]	[more]
gi\|659104371\|ref\|XP_008452921.1\|	6.8e-09	87.50	PREDICTED: probable alpha-amylase 2 isoform X3 [Cucumis melo]	[more]
gi\|645228846\|ref\|XP_008221185.1\|	9.8e-08	78.13	PREDICTED: probable alpha-amylase 2 [Prunus mume]	[more]

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004556	alpha-amylase activity
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh09G000750.1	CmoCh09G000750.1	mRNA

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmoCh09G000750	MELO3C017002.2	Melon (DHL92) v3.6.1	cmomedB026
CmoCh09G000750	CsaV3_4G037030	Cucumber (Chinese Long) v3	cmocucB0020
CmoCh09G000750	Bhi09G002425	Wax gourd	cmowgoB0023
CmoCh09G000750	CsGy4G025000	Cucumber (Gy14) v2	cgybcmoB438
CmoCh09G000750	Carg08649	Silver-seed gourd	carcmoB0144

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CmoCh09G000750	Watermelon (97103) v2	cmowmbB017
CmoCh09G000750	Cucurbita maxima (Rimu)	cmacmoB435
CmoCh09G000750	Cucurbita moschata (Rifu)	cmocmoB033
CmoCh09G000750	Cucurbita moschata (Rifu)	cmocmoB017
CmoCh09G000750	Cucumber (Gy14) v1	cgycmoB0742
CmoCh09G000750	Cucurbita maxima (Rimu)	cmacmoB027
CmoCh09G000750	Cucurbita maxima (Rimu)	cmacmoB755
CmoCh09G000750	Wild cucumber (PI 183967)	cmocpiB016
CmoCh09G000750	Cucumber (Chinese Long) v2	cmocuB015
CmoCh09G000750	Melon (DHL92) v3.5.1	cmomeB026
CmoCh09G000750	Watermelon (Charleston Gray)	cmowcgB018
CmoCh09G000750	Watermelon (97103) v1	cmowmB021
CmoCh09G000750	Cucurbita pepo (Zucchini)	cmocpeB020
CmoCh09G000750	Cucurbita pepo (Zucchini)	cmocpeB034
CmoCh09G000750	Bottle gourd (USVL1VR-Ls)	cmolsiB012
CmoCh09G000750	Silver-seed gourd	carcmoB0190
CmoCh09G000750	Silver-seed gourd	carcmoB0484