Cucsa.240430.1 (mRNA) Cucumber (Gy14) v1

BLAST of Cucsa.240430.1 vs. Swiss-Prot
Match: LORF2_HUMAN (LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens PE=1 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 1.0e-20
Identity = 100/357 (28.01%), Postives = 161/357 (45.10%), Query Frame = 1

Query: 483 EIYNALNTFSKNKSSGPDGFTTKFLKGTWKFTKENILKIFNDLHKNGII--NKIEKSYVL 542
           EI   +N+    KS GPDGFT +F +   +     +LK+F  + K GI+  +  E S +L
Sbjct: 457 EIVAIINSLPTKKSPGPDGFTAEFYQRYKEELVPFLLKLFQSIEKEGILPNSFYEASIIL 516

Query: 543 L-----------DYRPISLTTGLYKLIAKVIAERLKLVLPEIISENQLAF-RGRQITDAI 602
           +           ++RPISL     K++ K++A R++  + ++I  +Q+ F  G Q    I
Sbjct: 517 IPKPGRDTTKKENFRPISLMNIDAKILNKILANRIQQHIKKLIHHDQVGFIPGMQGWFNI 576

Query: 603 LIASEAVDFWKKKKVKGFV-VKLDIEKTFDKINWKFMDFMLLKKDFPQKWRKWIEAYLGK 662
             +   +    + K K  V + +D EK FDKI   FM   L K      + K I A   K
Sbjct: 577 RKSINVIQHINRAKDKNHVIISIDAEKAFDKIQQPFMLKTLNKLGIDGMYLKIIRAIYDK 636

Query: 663 IKPN--------------RGIRQGDHISPFIFVLAMDYLSTLLNHMEKQNHIKGVSFNGK 722
              N               G RQG  +SP +F + ++ L+  +    ++  IKG+   GK
Sbjct: 637 PTANIILNGQKLEAFPLKTGTRQGCPLSPLLFNIVLEVLARAI---RQEKEIKGIQL-GK 696

Query: 723 HNLTHILFAYDILLFLEDDDKTIDNMRFSFRLFELASGLNINLNKSTISPINTDAQRTNC 782
             +   LFA D++++LE+   +  N+      F   SG  IN+ KS     N + Q  + 
Sbjct: 697 EEVKLSLFADDMIVYLENPIVSAQNLLKLISNFSKVSGYKINVQKSQAFLYNNNRQTESQ 756

Query: 783 VAKKWGISINFLPIQYLGVPLGGKPSSRNFGIPNYHLSVLKP-LKSVYNDIEKIWRN 810
           +  +   +I    I+YLG+ L      ++    NY     KP LK +  D  K W+N
Sbjct: 757 IMGELPFTIASKRIKYLGIQL--TRDVKDLFKENY-----KPLLKEIKEDTNK-WKN 801

BLAST of Cucsa.240430.1 vs. Swiss-Prot
Match: LIN1_NYCCO (LINE-1 reverse transcriptase homolog OS=Nycticebus coucang PE=3 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 6.3e-18
Identity = 122/531 (22.98%), Postives = 213/531 (40.11%), Query Frame = 1

Query: 346 SFMGRLKQLSYTIKNWSKSLTKSNEEEIQTLLNELEHIDNLEVDSNITN----------- 405
           + MG LKQL    K    +   S  +EI  +  EL  I+N  +   I             
Sbjct: 315 NLMGHLKQLE---KEEHSNPKPSRRKEITKIRAELNEIENKRIIQQINKSKSWFFEKINK 374

Query: 406 -----LHITRRASIKTDLCQMAFKEAQIWAKKCKRLWNLEGDENYAFYHKICSARQRRSF 465
                 ++TR+  +K+ +  +     +I     +    L       + HK  + ++   +
Sbjct: 375 IDKPLANLTRKKRVKSLISSIRNGNDEITTDPSEIQKILNEYYKKLYSHKYENLKEIDQY 434

Query: 466 ISSISTAQGVLCSSDIPIKTTTHKDLCKMFYEEEIYNALNTFSKNKSSGPDGFTTKFLKG 525
           + +    +  L   ++ +       L +     EI + +    K KS GPDGFT++F + 
Sbjct: 435 LEACHLPR--LSQKEVEM-------LNRPISSSEIASTIQNLPKKKSPGPDGFTSEFYQT 494

Query: 526 TWKFTKENILKIFNDLHKNGII-NKIEKSYVLL------------DYRPISLTTGLYKLI 585
             +     +L +F ++ K GI+ N   ++ + L            +YRPISL     K++
Sbjct: 495 FKEELVPILLNLFQNIEKEGILPNTFYEANITLIPKPGKDPTRKENYRPISLMNIDAKIL 554

Query: 586 AKVIAERLKLVLPEIISENQLAF-RGRQITDAILIASEAVDFWKKKKVKG-FVVKLDIEK 645
            K++  R++  + +II  +Q+ F  G Q    I  +   +    K K K   ++ +D EK
Sbjct: 555 NKILTNRIQQHIKKIIHHDQVGFIPGSQGWFNIRKSINVIQHINKLKNKDHMILSIDAEK 614

Query: 646 TFDKINWKFMDFMLLKKDFPQKWRKWIEAYLGKIKPN--------------RGIRQGDHI 705
            FD I   FM   L K      + K IEA   K   N               G RQG  +
Sbjct: 615 AFDNIQHPFMIRTLKKIGIEGTFLKLIEAIYSKPTANIILNGVKLKSFPLRSGTRQGCPL 674

Query: 706 SPFIFVLAMDYLSTLLNHMEKQNHIKGVSFNGKHNLTHILFAYDILLFLEDDDKTIDNMR 765
           SP +F + M+ L+  +    ++  IKG+   G   +   LFA D++++LE+   +   + 
Sbjct: 675 SPLLFNIVMEVLAIAI---REEKAIKGIHI-GSEEIKLSLFADDMIVYLENTRDSTTKLL 734

Query: 766 FSFRLFELASGLNINLNKSTISPINTDAQRTNCVAKKWGISINFLPIQYLGVPLGGKPSS 825
              + +   SG  IN +KS       + Q    V      ++    ++YLGV L      
Sbjct: 735 EVIKEYSNVSGYKINTHKSVAFIYTNNNQAEKTVKDSIPFTVVPKKMKYLGVYL--TKDV 794

Query: 826 RNFGIPNYHLSVLKPLKSVYNDIEKIWRNF--LWRNTYEKKNINLIKWSTI 830
           ++    NY        K +  D+ K W+N    W        IN++K S +
Sbjct: 795 KDLYKENYETL----RKEIAEDVNK-WKNIPCSWLG-----RINIVKMSIL 817

BLAST of Cucsa.240430.1 vs. Swiss-Prot
Match: LORF2_MOUSE (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus GN=Pol PE=1 SV=2)

HSP 1 Score: 90.9 bits (224), Expect = 9.1e-17
Identity = 88/326 (26.99%), Postives = 145/326 (44.48%), Query Frame = 1

Query: 482 EEIYNALNTFSKNKSSGPDGFTTKFLKGTWKFTKENILKIFNDL-HKNGIINKIEKSYV- 541
           +EI   +N+    KS GPDGF+ +F    ++  KE+++ I + L HK  +   +  S+  
Sbjct: 463 KEIEAVINSLPTKKSPGPDGFSAEF----YQTFKEDLIPILHKLFHKIEVEGTLPNSFYE 522

Query: 542 ---------------LLDYRPISLTTGLYKLIAKVIAERLKLVLPEIISENQLAF-RGRQ 601
                          + ++RPISL     K++ K++A R++  +  II  +Q+ F  G Q
Sbjct: 523 ATITLIPKPQKDPTKIENFRPISLMNIDAKILNKILANRIQEHIKAIIHPDQVGFIPGMQ 582

Query: 602 ITDAILIASEAVDFWKKKKVKG-FVVKLDIEKTFDKINWKFMDFMLLKKDFPQKWRKWIE 661
               I  +   + +  K K K   ++ LD EK FDKI   FM  +L +      +   I+
Sbjct: 583 GWFNIRKSINVIHYINKLKDKNHMIISLDAEKAFDKIQHPFMIKVLERSGIQGPYLNMIK 642

Query: 662 AYLGK----IKPN----------RGIRQGDHISPFIFVLAMDYLSTLLNHMEKQNHIKGV 721
           A   K    IK N           G RQG  +SP++F + ++ L+  +    +Q  IKG+
Sbjct: 643 AIYSKPVANIKVNGEKLEAIPLKSGTRQGCPLSPYLFNIVLEVLARAI---RQQKEIKGI 702

Query: 722 SFNGKHNLTHILFAYDILLFLEDDDKTIDNMRFSFRLFELASGLNINLNKSTISPINTDA 775
              GK  +   L A D+++++ D   +   +      F    G  IN NKS       + 
Sbjct: 703 QI-GKEEVKISLLADDMIVYISDPKNSTRELLNLINSFGEVVGYKINSNKSMAFLYTKNK 762

BLAST of Cucsa.240430.1 vs. Swiss-Prot
Match: YTX2_XENLA (Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis PE=3 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 2.1e-13
Identity = 82/333 (24.62%), Postives = 156/333 (46.85%), Query Frame = 1

Query: 482 EEIYNALNTFSKNKSSGPDGFTTKFLKGTWKFTKENILKIFNDLHKNG---------IIN 541
           +E+  AL     NKS G DG T +F +  W     +  ++  +  K G         +++
Sbjct: 452 DELSQALRLMPHNKSPGLDGLTIEFFQFFWDTLGPDFHRVLTEAFKKGELPLSCRRAVLS 511

Query: 542 KIEKS---YVLLDYRPISLTTGLYKLIAKVIAERLKLVLPEIISENQ-LAFRGRQITDAI 601
            + K     ++ ++RP+SL +  YK++AK I+ RLK VL E+I  +Q     GR I D +
Sbjct: 512 LLPKKGDLRLIKNWRPVSLLSTDYKIVAKAISLRLKSVLAEVIHPDQSYTVPGRTIFDNV 571

Query: 602 LIASEAVDFWKKKKVKGFVVKLDIEKTFDKINWKFMDFMLLKKDFPQKWRKWIEAYLGK- 661
            +  + + F ++  +    + LD EK FD+++ +++   L    F  ++  +++      
Sbjct: 572 FLIRDLLHFARRTGLSLAFLSLDQEKAFDRVDHQYLIGTLQAYSFGPQFVGYLKTMYASA 631

Query: 662 ---IKPN----------RGIRQGDHISPFIFVLAMDYLSTLLNHMEKQNHIKGVSFNGKH 721
              +K N          RG+RQG  +S  ++ LA++    LL     +  + G+    + 
Sbjct: 632 ECLVKINWSLTAPLAFGRGVRQGCPLSGQLYSLAIEPFLCLL-----RKRLTGLVLK-EP 691

Query: 722 NLTHILFAY-DILLFLEDDDKTIDNMRFSFRLFELASGLNINLNKSTISPINTDAQRTNC 781
           ++  +L AY D ++ +  D   ++  +    ++  AS   IN +KS  S +   + + + 
Sbjct: 692 DMRVVLSAYADDVILVAQDLVDLERAQECQEVYAAASSARINWSKS--SGLLEGSLKVDF 751

Query: 782 VAKKW-GISINFLPIQYLGVPLGGK--PSSRNF 784
           +   +  IS     I+YLGV L  +  P S+NF
Sbjct: 752 LPPAFRDISWESKIIKYLGVYLSAEEYPVSQNF 776

BLAST of Cucsa.240430.1 vs. Swiss-Prot
Match: PO22_POPJA (Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2 (Fragment) OS=Popillia japonica PE=3 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 1.0e-07
Identity = 57/217 (26.27%), Postives = 103/217 (47.47%), Query Frame = 1

Query: 542 DYRPISLTTGLYKLIAKVIAERLKLVLPEIISENQLAFRGRQITDAILIASEAVDFW--- 601
           ++RPI++ + L +L+ +++A+RL+  +     E   A +G    D  L+ S  +D +   
Sbjct: 75  NWRPITIASALQRLLHRILAKRLEAAV-----ELHPAQKGYARIDGTLVNSLLLDTYISS 134

Query: 602 KKKKVKGF-VVKLDIEKTFDKINWKFMDFMLLKKDFPQKWRKWIEAYLG----------- 661
           ++++ K + VV LD+ K FD ++   +   L +    +    +I   L            
Sbjct: 135 RREQRKTYNVVSLDVRKAFDTVSHSSICRALQRLGIDEGTSNYITGSLSDSTTTIRVGPG 194

Query: 662 ----KIKPNRGIRQGDHISPFIFVLAMDYLSTLLNHMEKQNHIKGVSFNGKHNLTHILFA 721
               KI   RG++QGD +SPF+F   +D    LL  ++    I G    G+  +  + FA
Sbjct: 195 SQTRKICIRRGVKQGDPLSPFLFNAVLD---ELLCSLQSTPGIGGTI--GEEKIPVLAFA 254

Query: 722 YDILLFLEDDDKTIDNMRFSFRLFELASGLNINLNKS 740
            D+LL LED+D  +     +   F    G+++N  KS
Sbjct: 255 DDLLL-LEDNDVLLPTTLATVANFFRLRGMSLNAKKS 280

BLAST of Cucsa.240430.1 vs. TrEMBL
Match: A5B0H6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_006235 PE=4 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 8.3e-118
Identity = 301/1005 (29.95%), Postives = 465/1005 (46.27%), Query Frame = 1

Query: 204  RGDFWIELHEIKSICLPRWL--------------MAKSITFSCMNNFNTFISGCDFIDPP 263
            R DFW+EL +I  +  P W               M  S       +F++FIS C+ +DPP
Sbjct: 707  RKDFWVELFDIYGLTYPLWCVGGDFNVIRRSSEKMGGSSLTPSXRDFDSFISECELLDPP 766

Query: 264  LTNAKFTWSNLRAQPILSRLNIFLYTPNWEILFEPHFSKMLPRVTSDHFSIALESNSLKW 323
            L NA FTWSN++  P+  RL+ FLY+  W +LF     + L R TSDH+ I +++N   W
Sbjct: 767  LRNASFTWSNMQESPVCKRLDRFLYSNEWGLLFPQGLQEALIRRTSDHWPIVMDTNPFMW 826

Query: 324  GCSPFIFTNSFLKEVSFKQNIEVWWKNTAQVGHPGYSFMGRLKQLSYTIKNWSKSLTKSN 383
            G +PF F N +L   +FK+N   WW     +G  G  FM RL+ +   +K W KS     
Sbjct: 827  GPTPFRFENMWLXHTNFKENFRDWWSGFQGIGWEGXKFMRRLQYVKAKLKEWXKSSFGEL 886

Query: 384  EEEIQTLLNELEHIDNLEVDSNITNLHITRRASIKTDLCQMAFKEAQIWAKKCKRLWNLE 443
            +E+ +++LN+L + D +E +                +L ++  +E   W +K K  W  E
Sbjct: 887  KEKKKSILNDLANFDAIEQEGG--------------ELEELILREEIHWRQKAKVKWVKE 946

Query: 444  GDENYAFYHKICSARQRRSF------------------------------ISSISTAQGV 503
            GD N  FYHK+ + R+ R +                               +    + GV
Sbjct: 947  GDCNSKFYHKVXNGRRNRKYIKELENERGLVLKNAESITEEILHYFEKLYTNPTGESWGV 1006

Query: 504  LCSSDIPIKTTTHKDLCKMFYEEEIYNALNTFSKNKSSGPDGFTTKFLKGTWKFTKENIL 563
                  PI   +   L   F EEEI  A+    ++K+ GPDGFT    +  W   KE+++
Sbjct: 1007 EGLDWSPISEESALRLESPFTEEEISKAIFQLDRDKAXGPDGFTIAXFQECWDVIKEDLV 1066

Query: 564  KIFNDLHKNGIINK-IEKSYVLL-----------DYRPISLTTGLYKLIAKVIAERLKLV 623
            ++F + H++GIIN+    S++ L           D+RPISL T LYK+IAKV++ RL+ V
Sbjct: 1067 RVFAEFHRSGIINQSTNASFIXLIPKKSLSKRISDFRPISLITSLYKIIAKVLSGRLRGV 1126

Query: 624  LPEIISENQLAF-RGRQITDAILIASEAVDFWKKKKVKGFVVKLDIEKTFDKINWKFMDF 683
            L E I   Q AF +GRQI DA+LIA+E VD  ++   +G V K+D EK +D + W F+D 
Sbjct: 1127 LHETIHYTQGAFVQGRQILDAVLIANEIVDERRRSGEEGVVFKIDFEKAYDHVKWDFLDH 1186

Query: 684  MLLKKDFPQKWRKWIEAYL--------------GKIKPNRGIRQGDHISPFIFVLAMDYL 743
            +L KK F  +WRKW+   L              G +K +RG+RQGD +SPF+F L  D L
Sbjct: 1187 VLEKKGFSPRWRKWMSGCLSSVSFAILVNGSAKGWVKASRGLRQGDPLSPFLFTLVADVL 1246

Query: 744  STLLNHMEKQNHIKGVSFN-GKHNLTHILFAYDILLFLEDDDKTIDNMRFSFRLFELASG 803
            S +L   E++N ++G      +  ++H+ FA D + F    ++ +  ++    +F   SG
Sbjct: 1247 SKMLMRAEERNMLEGFRVGRNRTRVSHLQFADDTIFFSNSREEELQTLKSLLLVFGHISG 1306

Query: 804  LNINLNKSTISPINTDAQRTNCVAKKWGISINFLPIQYLGVPLGGKPSSRNFG------- 863
            L +NLNKS+I  IN D    + +A+      +  PI YLG+PLGG P +  F        
Sbjct: 1307 LKVNLNKSSIYGINLDQAHLSRLAEMLDCKASGWPILYLGLPLGGNPKACGFWDPVVERI 1366

Query: 864  ---------------------------IPNYHLSVLKPLKSVYNDIEKIWRNFLWRNTYE 923
                                       +P+Y LS+ K   +V   IE++ R+FLW    E
Sbjct: 1367 SSRLDGWQKAYLSFXGRITLIQSCLTHLPSYFLSLFKMPATVAAKIERLQRDFLWSGVGE 1426

Query: 924  KKNINLIKWSTILSPHK-GGLGINSVE------------NFMRKKNPLLKCIIIAKYEQS 983
             K  +L++W  +  P   GGLG+ ++              + R+ + L    I   +++ 
Sbjct: 1427 GKRDHLVRWDIVXRPKTIGGLGLGNISXRNLALLGKWLWRYPREGSALWHQAIAQVFQEF 1486

Query: 984  YL---------------GELPTKGKFSSSKAPRMF-IIKRVDWVLPQDLCPRRPLR---- 1043
             L                +L    +   ++ PR+F ++   +  +   L P RP      
Sbjct: 1487 SLITRYVVGNGDRIRFWEDLWRGDQXLGTQYPRLFRVVVDKNISISSVLXPSRPFLWNLN 1546

Query: 1044 -------SVEEALWGNMKAS-----LPSLPAFGFDIPRWNLNNNSSFTVASIKLARPLNN 1052
                   S  E L G M++       PS+P    D   W L+++  F+V S  LA   ++
Sbjct: 1547 FRRNLSDSEIEDLEGLMRSLDDLYLSPSVP----DARLWPLSSSGLFSVKSFFLALSQSS 1606

BLAST of Cucsa.240430.1 vs. TrEMBL
Match: A5AD48_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_026527 PE=4 SV=1)

HSP 1 Score: 422.2 bits (1084), Expect = 1.9e-114
Identity = 302/1051 (28.73%), Postives = 492/1051 (46.81%), Query Frame = 1

Query: 98   KKKLMAWLKNNNPKLTTNSFVDIENEDREAISEVNVMNFSISVKWHFSPAKSSSGGIVIM 157
            ++ +  +L++ NP +        EN DR  +  V  +    +  W   P   +SGGI+I+
Sbjct: 17   RRMVKDFLRSENPDVVMIQETKKENCDRRFVGSVWTVR---NKDWVALPVSGASGGILII 76

Query: 158  WDDIRFKVVDYLVKKFTLSIEITYPNDSNNLNWWLTVVCGPSKRENRGDFWIELHEIKSI 217
            WD    +  + ++  F++S++ +          W++ V GP+    R DFW+EL +I  +
Sbjct: 77   WDSKNLRREEVVIGSFSISVKFSLDGCGP---LWISAVYGPNSPSLRKDFWVELFDIYGL 136

Query: 218  CLPRW---------------LMAKSITFSCMNNFNTFISGCDFIDPPLTNAKFTWSNLRA 277
                W               L   S+T S M +F++FI  C+ +DPPL NA FTWSN++ 
Sbjct: 137  TYLLWCVGGDFNVIRRSSEKLGGSSLTPS-MRDFDSFIRECELLDPPLRNASFTWSNMQE 196

Query: 278  QPILSRLNIFLYTPNWEILFEPHFSKMLPRVTSDHFSIALESNSLKWGCSPFIFTNSFLK 337
             P+  RL+ FLY+  W +LF     + L R TSDH+ I +++N  +WG +PF F N +L+
Sbjct: 197  SPVCKRLDRFLYSNEWGLLFPQGLQEALIRRTSDHWPIVMDTNPFRWGPTPFRFENMWLQ 256

Query: 338  EVSFKQNIEVWWKNTAQVGHPGYSFMGRLKQLSYTIKNWSKSLTKSNEEEIQTLLNELEH 397
              +FK+N   WW      G  G+ FM RL+ +   +K W+K      +E+  ++LN+L +
Sbjct: 257  HTNFKENFRDWWSGFQGNGWEGHKFMRRLQYVKAKLKEWNKFSFGELKEKKNSILNDLAN 316

Query: 398  IDNLEVDSNITNLHITRRASIKTDLCQMAFKEAQIWAKKCKRLWNLEGDENYAFYHKICS 457
             D +E +  +  + ++ RAS K +L ++  +E   W +K K  W  EGD N  FYHK+ +
Sbjct: 317  FDAIEQEGGLNPVLLSXRASRKGELEELILREEIHWRQKAKVKWVKEGDCNSKFYHKVAN 376

Query: 458  ARQRRSF------------------------------ISSISTAQGVLCSSDIPIKTTTH 517
             ++ R +                               +    + GV       I   + 
Sbjct: 377  GKRNRKYXKELENERGLVLKNAESITXEILHYFEKLYTNPTGESWGVEGLDWSLISEESA 436

Query: 518  KDLCKMFYEEEIYNALNTFSKNKSSGPDGFTTKFLKGTWKFTKENILKIFNDLHKNGIIN 577
              L   F EEEI  A+    ++K+ GPDGFT    +  W   KE+++++F + H++GIIN
Sbjct: 437  LRLDSPFTEEEISKAIFQLDRDKALGPDGFTIAVFQECWDVIKEDLVRVFAEFHRSGIIN 496

Query: 578  K-IEKSYVLL-----------DYRPISLTTGLYKLIAKVIAERLKLVLPEIISENQLAF- 637
            +    S+++L           D+RPISL T LYK+IAKV+  RL+ VL E I   Q AF 
Sbjct: 497  QSTNASFIVLIPKKCLSKRISDFRPISLITSLYKIIAKVLFGRLRGVLHETIHYTQGAFV 556

Query: 638  RGRQITDAILIASEAVDFWKKKKVKGFVVKLDIEKTFDKINWKFMDFMLLKKDFPQKWRK 697
            +GRQI  A+LIA+E VD  ++   +G V K+D EK +D + W F+D +L KK F  +WRK
Sbjct: 557  QGRQILHAVLIANEIVDERRRSGEEGVVFKIDFEKAYDHVKWDFLDHVLEKKGFSPRWRK 616

Query: 698  WIEAYL--------------GKIKPNRGIRQGDHISPFIFVLAMDYLSTLLNHMEKQNHI 757
            W+   L              G +K +RG+RQGD +SPF+F L  D LS +L   E++N +
Sbjct: 617  WMSGCLSSVSYAILVNGSAKGWVKASRGLRQGDPLSPFLFTLVADVLSRMLMRAEERNMM 676

Query: 758  KGVSFN-GKHNLTHILFAYDILLFLEDDDKTIDNMRFSFRLFELASGLNINLNKSTISPI 817
            +G      K  ++H+ FA D + F    ++ +  ++    +F   SGL +NLNKS+I  I
Sbjct: 677  EGFRVGRNKTRVSHLQFADDTIFFSNSREEELQTLKSLLLVFGHISGLKVNLNKSSIYGI 736

Query: 818  ------------NTDAQRTNCVAKKWGISINFLP---------IQYLGVPLGG-KPSSRN 877
                          D + +        + +   P         ++ +   L G +    +
Sbjct: 737  NLDQAHLSRLAEMLDCKASGWPILYLSLPLGGNPKACGFWDPVVERISSRLDGWQKPYLS 796

Query: 878  FG------------IPNYHLSVLKPLKSVYNDIEKIWRNFLWRNTYEKKNINLIKWSTIL 937
            FG            +P+Y LS+ K    V   IE+I R+FLW   + ++  +  +    +
Sbjct: 797  FGGRITLIQSCLTHLPSYFLSLFKIPALVAAKIERIQRDFLWSRGWGRQKGSFSEVGCCV 856

Query: 938  SPHKGGLGINSVENFMRKKNPLLKCIIIAKYEQSYLGELPTKGKFSSSKAPRMFIIKRVD 997
               K   G    E F+ +     + ++  +  +  + +  +         P ++      
Sbjct: 857  QT-KDNRGFGFGEYFLEESRSSREMVMEPRLFRVVVDKNISISSVLGPSRPFLW------ 916

Query: 998  WVLPQDLCPRRPLRSVE-EALWGNMKAS-----LPSLPAFGFDIPRWNLNNNSSFTVASI 1036
                 +L  RR L   E E L G M++       PS+P    D   W L+++  F+V S 
Sbjct: 917  -----NLNFRRNLSDSEIEDLEGLMRSLDDLYLSPSVP----DARLWPLSSSGLFSVKSF 976

BLAST of Cucsa.240430.1 vs. TrEMBL
Match: A5BSZ6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_024106 PE=4 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 3.6e-113
Identity = 252/782 (32.23%), Postives = 397/782 (50.77%), Query Frame = 1

Query: 121  ENEDREAISEVNVMNFSISVKWHFSPAKSSSGGIVIMWDDIRFKVVDYLVKKFTLSIEIT 180
            EN DR  +  V  +    +  W   PA  +SGGI+I+WD       + ++  F++S++ +
Sbjct: 675  ENCDRRFVGSVWTVR---NKDWVALPASGASGGILIIWDSKILSREEVVIGSFSVSVKFS 734

Query: 181  YPNDSNNLNWWLTVVCGPSKRENRGDFWIELHEIKSICLPRWL--------------MAK 240
                      W++ V GP+    R DFW+EL +I  +  P W               M  
Sbjct: 735  LDGCGP---LWISAVYGPNSPSLRKDFWVELFDIYGLTYPLWCVGGDFNVIRRSSEKMGG 794

Query: 241  SITFSCMNNFNTFISGCDFIDPPLTNAKFTWSNLRAQPILSRLNIFLYTPNWEILFEPHF 300
            S     M +F++FIS C+ +DPPL NA FTWSN++  P+  RL+ FLY+  W +LF    
Sbjct: 795  SSLTPSMRDFDSFISECELLDPPLRNASFTWSNMQESPVCKRLDRFLYSNEWGLLFPQGL 854

Query: 301  SKMLPRVTSDHFSIALESNSLKWGCSPFIFTNSFLKEVSFKQNIEVWWKNTAQVGHPGYS 360
             + L R TSDH+ I +++N   WG +PF F N +L+  +FK+N   WW     +G  G+ 
Sbjct: 855  QEALIRRTSDHWPIVMDTNPFMWGPTPFRFENMWLQHTNFKENFRDWWSGFQGIGWEGHK 914

Query: 361  FMGRLKQLSYTIKNWSKSLTKSNEEEIQTLLNELEHIDNLEVDSNITNLHITRRASIKTD 420
            FM RL+ +   +K W+KS     +E+ +++LN+L + D +E +  +    I++R S K +
Sbjct: 915  FMRRLQYVKAKLKEWNKSSFGELKEKKKSILNDLANFDAIEQEGGLNPDLISQRVSRKGE 974

Query: 421  LCQMAFKEAQIWAKKCKRLWNLEGDENYAFYHKICSARQRRSFISSISTAQGVLCSSD-- 480
            L ++  +E   W +K K  W  EGD N  FYHK+ + R+ R +I  +   +G++  +   
Sbjct: 975  LEELILREEIHWRQKAKVKWVKEGDCNSKFYHKVANGRRNRKYIKELENERGLVLKNAER 1034

Query: 481  ----------IPIKTTTHKDLCKMFYEEEIYNALNTFSKNKSSGPDGFTTKFLKGTWKFT 540
                       PI   +   L   F EEEI  A+    ++K+ GPDGFT    +  W   
Sbjct: 1035 ESWGVEGLDWSPISEESALRLESPFTEEEISKAIFQLDRBKAPGPDGFTIAVFQECWDVI 1094

Query: 541  KENILKIFNDLHKNGIINK-IEKSYVLL-----------DYRPISLTTGLYKLIAKVIAE 600
            KE+++++F + H++GIIN+    S+++L           D+RPISL T LYK+IAKV++ 
Sbjct: 1095 KEDLVRVFAEFHRSGIINQSTNASFIVLIPKKSLSKRISDFRPISLITSLYKIIAKVLSG 1154

Query: 601  RLKLVLPEIISENQLAFRGRQITDAILIASEAVDFWKKKKVKGFVVKLDIEKTFDKINWK 660
            RL+               G +  +A+LIA+E VD  ++   +G V K+D EK +D + W 
Sbjct: 1155 RLR--------------GGDKYWNAVLIANEIVDERRRSXEEGVVFKIDFEKAYDHVKWD 1214

Query: 661  FMDFMLLKKDFPQKWRKWIEAYL--------------GKIKPNRGIRQGDHISPFIFVLA 720
            F+D +L KK F  +WRKW+   L              G +K +RG+RQGD +SPF+F L 
Sbjct: 1215 FLDHVLEKKGFSPRWRKWMSGCLSSVSFAILVNGSAKGWVKASRGLRQGDPLSPFLFTLV 1274

Query: 721  MDYLSTLLNHMEKQNHIKGVSF-NGKHNLTHILFAYDILLFLEDDDKTIDNMRFSFRLFE 780
             D LS +L   E++N ++G      +  ++H+ FA D + F    ++ +  ++    +F 
Sbjct: 1275 ADVLSRMLMRAEERNMLEGFRVGRNRTRVSHLQFADDTIFFSNSREEELQTLKSLLLVFG 1334

Query: 781  LASGLNINLNKSTISPINTDAQRTNCVAKKWGISINFLPIQYLGVPLGGKPSSRNFGIPN 840
              SGL +NLNKS+I  IN D    + +A+      +  PI YLG+PLGG P +  F  P 
Sbjct: 1335 HISGLKVNLNKSSIYGINLDQAHLSRLAEMLDCKASGWPILYLGLPLGGNPKACGFWDPV 1394

Query: 841  YHLSVLKPLKSVYNDIEKI-WRN------FLWRNTYEKKNINLIKWSTILSPHKGGLGIN 843
                + KP       +  I WRN      +LWR   E   +      +I   H  G   N
Sbjct: 1395 VE-RISKPKTIGGLGLGNISWRNLALLGKWLWRYPREGSXLWHQVILSIYGSHSNGWDAN 1435

BLAST of Cucsa.240430.1 vs. TrEMBL
Match: A5BSZ6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_024106 PE=4 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 5.0e-06
Identity = 42/139 (30.22%), Postives = 64/139 (46.04%), Query Frame = 1

Query: 919  PSLPAFGFDIPRWNLNNNSSFTVASIKLARPLNNQAEVNYNDGVIYNNVWKSSIPKMCKL 978
            PS+P    D   W L+++  F+V S  LA   ++ +  N+        VW S +P   K 
Sbjct: 1541 PSVP----DARLWPLSSSGLFSVKSFFLALSQSSGSSQNFPSKF----VWNSQVPFKVKS 1600

Query: 979  FLWTLIQECINTTDMLQRRLPTWNIKPSWCTLCKAAEEDRNHLFSLYPFSSKLWQ----- 1038
            F+W +  + +NT DMLQ R P   + P  C LC    E  +HLF     +  LW      
Sbjct: 1601 FVWLVAHKKVNTNDMLQVRRPYKALSPDICILCMKHGESXDHLFLHCSLTIGLWHRLFQL 1660

Query: 1039 -KVEVILDRPLYPINSPLF 1052
             K++ +  R +Y + S  F
Sbjct: 1661 AKLDWVSPRSIYDMMSIKF 1671

HSP 2 Score: 413.7 bits (1062), Expect = 6.8e-112
Identity = 244/705 (34.61%), Postives = 369/705 (52.34%), Query Frame = 1

Query: 124  DREAISEVNVMNFSISVKWHFSPAKSSSGGIVIMWDDIRFKVVDYLVKKFTLSIEITYPN 183
            DR+ ++ V    F    +W FSP+   SGGI ++W+     V+D +V +F++SI I    
Sbjct: 365  DRQLVAGVWGSRFK---EWVFSPSLGRSGGIAVLWNSQSVSVIDSMVGEFSVSIRI---E 424

Query: 184  DSNNLNWWLTVVCGPSKRENRGDFWIELHEIKSICLPRWLMAKS---ITFSC-------- 243
            ++   +WWL+ + GP ++  R  FW EL ++   C   W +      + FS         
Sbjct: 425  ENIGTDWWLSGIYGPCRQRERNSFWEELADLYGYCGDMWCLGGDFNVVRFSAEKSNEGRV 484

Query: 244  ---MNNFNTFISGCDFIDPPLTNAKFTWSNLRAQPILSRLNIFLYTPNWEILFEPHFSKM 303
               M +FN FI   +  DP L NA FTWSNLR   +  RL+ FL + +WE  F  +  K 
Sbjct: 485  TKSMRDFNDFIQETNLRDPILLNASFTWSNLRENAVCRRLDRFLVSGSWEEHFPHYRHKA 544

Query: 304  LPRVTSDHFSIALESNSLKWGCSPFIFTNSFLKEVSFKQNIEVWWKNTAQVGHPGYSFMG 363
            LPR+TSDH  I L+++ +KWG SPF F N +L    FK+ I++WW      G  GY FM 
Sbjct: 545  LPRITSDHCPIELDTSRVKWGPSPFRFENMWLNHPDFKRKIKLWWGEDQIPGWEGYKFMT 604

Query: 364  RLKQLSYTIKNWSKSLTKSNEEEIQTLLNELEHIDNLEVDSNITNLHITRRASIKTDLCQ 423
            RLK L   +K WSK      E +++     L  +D  E    + +L  + R ++   +  
Sbjct: 605  RLKMLKSKLKVWSKEEFGDVERDLREAEARLLVLDQREGTEGLDHLLRSERDNLLLKIGD 664

Query: 424  MAFKEAQIWAKKCKRLWNLEGDENYAFYHKICSARQRRSFISSISTAQGVLCSSDIPIKT 483
            +A KE   W ++ K  W  +GD N  F+H++ +  ++R++I  +      +   D  I+ 
Sbjct: 665  LAQKEEVKWRQRGKVKWARDGDGNTKFFHRVANGARKRNYIEKLEVEDLGVIEVDANIER 724

Query: 484  TTHKDLCKMFYEEEIYNALNTFSKNKSSGPDGFTTKFLKGTWKFTKENILKIFNDLHKNG 543
               +   K  Y      A+    K+KS GPDGF+  F +  W+  K +++K+  D  ++G
Sbjct: 725  EVIR-FFKGLYSSNKNKAVFDCGKDKSPGPDGFSMSFFQSCWEVVKGDLMKVMQDFFQSG 784

Query: 544  IIN------------KIEKSYVLLDYRPISLTTGLYKLIAKVIAERLKLVLPEIISENQL 603
            I+N            K   S  + DYRPISL T LYK+I+KV+A  L+ VL   IS++Q 
Sbjct: 785  IVNGVTNETFICLIPKKANSVKVTDYRPISLVTSLYKVISKVLASSLREVLGNTISQSQG 844

Query: 604  AF-RGRQITDAILIASEAVDFWKKKKVKGFVVKLDIEKTFDKINWKFMDFMLLKKDFPQK 663
            AF + RQI DA+L+A+E V+  +K+K KG V K+D EK +D + W F+D ++ +K F  K
Sbjct: 845  AFVQKRQILDAVLVANEVVEEVRKQKRKGLVFKIDFEKAYDHVEWNFVDDVMARKGFGVK 904

Query: 664  WRKWIEAYL--------------GKIKPNRGIRQGDHISPFIFVLAMDYLSTLLNHMEKQ 723
            WR WI   L              GK + +RG+RQGD +SPF+F L  D LS L+   +  
Sbjct: 905  WRGWIIGCLESVNFSIMINGKPRGKFRASRGLRQGDPLSPFLFTLVSDVLSRLIERAQDV 964

Query: 724  NHIKG-VSFNGKHNLTHILFAYDILLFLEDDDKTIDNMRFSFRLFELASGLNINLNKSTI 783
            N + G VS + +  ++H+ FA D +  L+  ++   N+    +LF   SG+ IN  KS I
Sbjct: 965  NLVHGIVSGHDQVEVSHLQFADDTIFLLDGKEEYWLNLLQLLKLFCDVSGMKINKAKSCI 1024

Query: 784  SPINTDAQRTNCVAKKWGISINFLPIQYLGVPLGGKPSSRNFGIP 787
              IN      N +A  WG  +   P+ YLG+PLGG P + NF  P
Sbjct: 1025 LGINFSTDVLNNMAGSWGCEVGCWPMVYLGLPLGGNPRALNFWNP 1062

BLAST of Cucsa.240430.1 vs. TrEMBL
Match: M5WJ76_PRUPE (Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa015871mg PE=4 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 1.6e-07
Identity = 28/72 (38.89%), Postives = 42/72 (58.33%), Query Frame = 1

Query: 964  YNNVWKSSIPKMCKLFLWTLIQECINTTDMLQRRLPTWNIKPSWCTLCKAAEEDRNHLFS 1023
            ++++WK+  P   + F+W      INT D +QRR P   + PSWC LCK   E+ +HLF 
Sbjct: 1327 FSSIWKAKTPPKIQFFVWLAANGRINTCDCIQRRQPKMCLSPSWCVLCKENAENIDHLFI 1386

Query: 1024 LYPFSSKLWQKV 1036
               +S +LW K+
Sbjct: 1387 HCSYSLRLWWKM 1398

HSP 2 Score: 48.5 bits (114), Expect = 5.8e-02
Identity = 23/59 (38.98%), Postives = 36/59 (61.02%), Query Frame = 1

Query: 785  IPNYHLSVLKPLKSVYNDIEKIWRNFLWRNTYEKKNINLIKWSTIL-SPHKGGLGINSV 843
            IP+Y++S+ K    V   +E++ RNFLW    E K  +L++W  +  S  +GGLGI S+
Sbjct: 1095 IPSYYMSLFKMPIGVAAKVEQLMRNFLWEGLDEGKKCHLVRWERVTKSKEEGGLGIGSL 1153

HSP 3 Score: 409.1 bits (1050), Expect = 1.7e-110
Identity = 243/717 (33.89%), Postives = 379/717 (52.86%), Query Frame = 1

Query: 142  WHFSPAKSSSGGIVIMWDDIRFKVVDYLVKKFTLSIEITYPNDSNNLNWWLTVVCGPSKR 201
            W   PA  +SGGI+I+WD  +    + ++  F++SI+    N   +L  WL+ V GP+  
Sbjct: 721  WAALPACGASGGILIIWDAKKLSREEVVLGSFSVSIKFAL-NGCESL--WLSAVYGPNIS 780

Query: 202  ENRGDFWIELHEIKSICLPRWLMAKSITF--------------SCMNNFNTFISGCDFID 261
              R DFW+EL +I  +  PRW +                      M  F+ FIS C+ ID
Sbjct: 781  ALRKDFWVELSDIAGLASPRWCVGGDFNVIRRSSEKLGGSRXTPSMKXFDDFISDCELID 840

Query: 262  PPLTNAKFTWSNLRAQPILSRLNIFLYTPNWEILFEPHFSKMLPRVTSDHFSIALESNSL 321
             PL +A FTWSN++   +  RL+ FLY+  WE  F      +LPR TSDH+ I LE+N  
Sbjct: 841  LPLRSASFTWSNMQVNXVCKRLDRFLYSNEWEQAFPQSIQGVLPRWTSDHWPIVLETNPF 900

Query: 322  KWGCSPFIFTNSFLKEVSFKQNIEVWWKNTAQVGHPGYSFMGRLKQLSYTIKNWSKSLTK 381
            KWG +PF F N +L+  SFK+N   WW+     G  G+ FM +L+ +   +K W+K+   
Sbjct: 901  KWGPTPFRFENMWLQHPSFKENFGRWWREFQGNGWEGHKFMRKLQFVKAKLKVWNKASFG 960

Query: 382  SNEEEIQTLLNELEHIDNLEVDSNITNLHITRRASIKTDLCQMAFKEAQIWAKKCKRLWN 441
               +  + +L++L + D+LE +  +++  + +RA  K +L ++  +E   W +K +  W 
Sbjct: 961  ELSKRKEDILSDLVNFDSLEQEGGLSHELLAQRALKKGELEELILREEIHWRQKARVKWV 1020

Query: 442  LEGDENYAFYHKICSARQRRSFI------------SSISTAQGVLCSSD----------- 501
             EGD N  F+HK+ + R+ R FI            +S S  + +L   +           
Sbjct: 1021 KEGDCNSRFFHKVANGRRNRKFIKELENENGLMMNNSESIKEEILRYFEKLYTSPSGESW 1080

Query: 502  -------IPIKTTTHKDLCKMFYEEEIYNALNTFSKNKSSGPDGFTTKFLKGTWKFTKEN 561
                    PI   +   L   F EEEI+ A+    ++K+ GPDGFT    +  W+  KE+
Sbjct: 1081 RVEGLDWSPISGESAFRLESPFTEEEIFKAIFQMDRDKAPGPDGFTIAVFQDCWEVIKED 1140

Query: 562  ILKIFNDLHKNGIINK-IEKSYVLL-----------DYRPISLTTGLYKLIAKVIAERLK 621
            ++K+F + H++GIIN+    S+++L           D+RPISL T LYK+IAKV+A R++
Sbjct: 1141 LVKVFTEFHRSGIINQSTNASFIVLLPKKSMSRRISDFRPISLITSLYKIIAKVLAGRIR 1200

Query: 622  LVLPEIISENQLAF-RGRQITDAILIASEAVDFWKKKKVKGFVVKLDIEKTFDKINWKFM 681
             VL E I   Q AF +GRQI DA+LIA+E VD  ++   +G V K+D EK +D ++W F+
Sbjct: 1201 GVLHETIHSTQGAFVQGRQILDAVLIANEIVDEKRRSGEEGVVFKIDFEKAYDHVSWDFL 1260

Query: 682  DFMLLKKDFPQKWRKWIEAYL--------------GKIKPNRGIRQGDHISPFIFVLAMD 741
            D +L  K F  +WRKW+   L              G +K +RG+RQGD +SPF+F +  D
Sbjct: 1261 DHVLEMKGFGIRWRKWMRGCLSSVSFAVLVNGNAKGWVKASRGLRQGDPLSPFLFTIVAD 1320

Query: 742  YLSTLLNHMEKQNHIKGVSF-NGKHNLTHILFAYDILLFLEDDDKTIDNMRFSFRLFELA 787
             LS +L   E++N ++G      +  ++H+ FA D + F    ++ +  ++    +F   
Sbjct: 1321 VLSRMLLKAEERNVLEGFKVGRNRTRVSHLQFADDTIFFSSSREEDMMTLKNVLLVFGHI 1380

BLAST of Cucsa.240430.1 vs. TAIR10
Match: AT1G43760.1 (AT1G43760.1 DNAse I-like superfamily protein)

HSP 1 Score: 70.5 bits (171), Expect = 7.2e-12
Identity = 84/387 (21.71%), Postives = 148/387 (38.24%), Query Frame = 1

Query: 223 LMAKSITFSCMNNFNTFISGCDFIDPPLTNAKFTWSNLRAQ-PILSRLNIFLYTPNWEIL 282
           ++  SI    +  F   +   D +D P     +TWSN +   PI+ +L+  +   +W   
Sbjct: 239 VLQTSIPMRGLEEFQNCLRDSDLVDIPSRGVHYTWSNHQDDNPIIRKLDRAIANGDWFSS 298

Query: 283 FEPHFSKMLPRVTSDHFS-IALESNSLKWGCSPFIFTNSFLKEVSFKQNIEVWWKNTAQV 342
           F    +       SDH   I +  N  K     F + +      +F  ++ V W+    V
Sbjct: 299 FPSAIAVFELSGVSDHSPCIIILENLPKRSKKCFRYFSFLSTHPTFLVSLTVAWEEQIPV 358

Query: 343 GHPGYSFMGRLKQLSYTIKNWSKSLTKSNEEEIQTLLNELEHIDNLEVDSNITNL----H 402
           G   +S    LK      K  ++    + + + +  L+ LE I +  + +   +L    H
Sbjct: 359 GSHMFSLGEHLKAAKKCCKLLNRQGFGNIQHKTKEALDSLESIQSQLLTNPSDSLFRVEH 418

Query: 403 ITRRASIKTDLCQMAFKEAQIWAKKCKRLWNLEGDENYAFYHKICSARQRRSFI------ 462
           + R+   K +    A +    + +K +  W  +GD N  F+HK+  A Q ++ I      
Sbjct: 419 VARK---KWNFFAAALES--FYRQKSRIKWLQDGDANTRFFHKVILANQAKNLIKFLRMD 478

Query: 463 --------------------------SSISTAQGVLCSSDI-PIKT--TTHKDLCKMFYE 522
                                     S I T   V    DI P +   T    L  +  +
Sbjct: 479 DDVRVENVTQVKEMIVAYYTHLLGSDSDILTPDSVQRIKDIHPFRCNDTLASRLSALPSD 538

Query: 523 EEIYNALNTFSKNKSSGPDGFTTKFLKGTWKFTKENILKIFNDLHKNG------------ 557
           +EI  A+    +NK+ GPD FT +F   +W   K++ +    +  + G            
Sbjct: 539 KEITAAVFAMPRNKAPGPDSFTAEFFWESWFVVKDSTIAAVKEFFRTGHLLKRFNATAIT 598

BLAST of Cucsa.240430.1 vs. TAIR10
Match: AT4G20520.1 (AT4G20520.1 RNA binding;RNA-directed DNA polymerases)

HSP 1 Score: 58.5 bits (140), Expect = 2.8e-08
Identity = 32/81 (39.51%), Postives = 51/81 (62.96%), Query Frame = 1

Query: 560 IAERLKLVLPEIISENQLAF-RGRQITDAILIASEAVDFWKKKK-VKGFVV-KLDIEKTF 619
           + ERLK ++  +I   Q +F  GR  TD I+   EAV   ++KK VKG+++ KLD+EK +
Sbjct: 1   MVERLKPLMTNLIGPAQASFIPGRVSTDNIVFVQEAVHSMRRKKGVKGWMLLKLDLEKAY 60

Query: 620 DKINWKFMDFMLLKKDFPQKW 638
           D+I W +++  L+   FP+ W
Sbjct: 61  DRIRWDYLEDTLISAGFPEVW 81

BLAST of Cucsa.240430.1 vs. TAIR10
Match: ATMG01250.1 (ATMG01250.1 RNA-directed DNA polymerase (reverse transcriptase))

HSP 1 Score: 52.0 bits (123), Expect = 2.6e-06
Identity = 23/60 (38.33%), Postives = 37/60 (61.67%), Query Frame = 1

Query: 646 GKIKPNRGIRQGDHISPFIFVLAMDYLSTLLNHMEKQNHIKGVSF-NGKHNLTHILFAYD 705
           G + P+RG+RQGD +SP++F+L  + LS L    ++Q  + G+   N    + H+LFA D
Sbjct: 20  GLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNSPRINHLLFADD 79

BLAST of Cucsa.240430.1 vs. NCBI nr
Match: gi|147776600|emb|CAN74004.1| (hypothetical protein VITISV_006235 [Vitis vinifera])

HSP 1 Score: 433.3 bits (1113), Expect = 1.2e-117
Identity = 301/1005 (29.95%), Postives = 465/1005 (46.27%), Query Frame = 1

Query: 204  RGDFWIELHEIKSICLPRWL--------------MAKSITFSCMNNFNTFISGCDFIDPP 263
            R DFW+EL +I  +  P W               M  S       +F++FIS C+ +DPP
Sbjct: 707  RKDFWVELFDIYGLTYPLWCVGGDFNVIRRSSEKMGGSSLTPSXRDFDSFISECELLDPP 766

Query: 264  LTNAKFTWSNLRAQPILSRLNIFLYTPNWEILFEPHFSKMLPRVTSDHFSIALESNSLKW 323
            L NA FTWSN++  P+  RL+ FLY+  W +LF     + L R TSDH+ I +++N   W
Sbjct: 767  LRNASFTWSNMQESPVCKRLDRFLYSNEWGLLFPQGLQEALIRRTSDHWPIVMDTNPFMW 826

Query: 324  GCSPFIFTNSFLKEVSFKQNIEVWWKNTAQVGHPGYSFMGRLKQLSYTIKNWSKSLTKSN 383
            G +PF F N +L   +FK+N   WW     +G  G  FM RL+ +   +K W KS     
Sbjct: 827  GPTPFRFENMWLXHTNFKENFRDWWSGFQGIGWEGXKFMRRLQYVKAKLKEWXKSSFGEL 886

Query: 384  EEEIQTLLNELEHIDNLEVDSNITNLHITRRASIKTDLCQMAFKEAQIWAKKCKRLWNLE 443
            +E+ +++LN+L + D +E +                +L ++  +E   W +K K  W  E
Sbjct: 887  KEKKKSILNDLANFDAIEQEGG--------------ELEELILREEIHWRQKAKVKWVKE 946

Query: 444  GDENYAFYHKICSARQRRSF------------------------------ISSISTAQGV 503
            GD N  FYHK+ + R+ R +                               +    + GV
Sbjct: 947  GDCNSKFYHKVXNGRRNRKYIKELENERGLVLKNAESITEEILHYFEKLYTNPTGESWGV 1006

Query: 504  LCSSDIPIKTTTHKDLCKMFYEEEIYNALNTFSKNKSSGPDGFTTKFLKGTWKFTKENIL 563
                  PI   +   L   F EEEI  A+    ++K+ GPDGFT    +  W   KE+++
Sbjct: 1007 EGLDWSPISEESALRLESPFTEEEISKAIFQLDRDKAXGPDGFTIAXFQECWDVIKEDLV 1066

Query: 564  KIFNDLHKNGIINK-IEKSYVLL-----------DYRPISLTTGLYKLIAKVIAERLKLV 623
            ++F + H++GIIN+    S++ L           D+RPISL T LYK+IAKV++ RL+ V
Sbjct: 1067 RVFAEFHRSGIINQSTNASFIXLIPKKSLSKRISDFRPISLITSLYKIIAKVLSGRLRGV 1126

Query: 624  LPEIISENQLAF-RGRQITDAILIASEAVDFWKKKKVKGFVVKLDIEKTFDKINWKFMDF 683
            L E I   Q AF +GRQI DA+LIA+E VD  ++   +G V K+D EK +D + W F+D 
Sbjct: 1127 LHETIHYTQGAFVQGRQILDAVLIANEIVDERRRSGEEGVVFKIDFEKAYDHVKWDFLDH 1186

Query: 684  MLLKKDFPQKWRKWIEAYL--------------GKIKPNRGIRQGDHISPFIFVLAMDYL 743
            +L KK F  +WRKW+   L              G +K +RG+RQGD +SPF+F L  D L
Sbjct: 1187 VLEKKGFSPRWRKWMSGCLSSVSFAILVNGSAKGWVKASRGLRQGDPLSPFLFTLVADVL 1246

Query: 744  STLLNHMEKQNHIKGVSFN-GKHNLTHILFAYDILLFLEDDDKTIDNMRFSFRLFELASG 803
            S +L   E++N ++G      +  ++H+ FA D + F    ++ +  ++    +F   SG
Sbjct: 1247 SKMLMRAEERNMLEGFRVGRNRTRVSHLQFADDTIFFSNSREEELQTLKSLLLVFGHISG 1306

Query: 804  LNINLNKSTISPINTDAQRTNCVAKKWGISINFLPIQYLGVPLGGKPSSRNFG------- 863
            L +NLNKS+I  IN D    + +A+      +  PI YLG+PLGG P +  F        
Sbjct: 1307 LKVNLNKSSIYGINLDQAHLSRLAEMLDCKASGWPILYLGLPLGGNPKACGFWDPVVERI 1366

Query: 864  ---------------------------IPNYHLSVLKPLKSVYNDIEKIWRNFLWRNTYE 923
                                       +P+Y LS+ K   +V   IE++ R+FLW    E
Sbjct: 1367 SSRLDGWQKAYLSFXGRITLIQSCLTHLPSYFLSLFKMPATVAAKIERLQRDFLWSGVGE 1426

Query: 924  KKNINLIKWSTILSPHK-GGLGINSVE------------NFMRKKNPLLKCIIIAKYEQS 983
             K  +L++W  +  P   GGLG+ ++              + R+ + L    I   +++ 
Sbjct: 1427 GKRDHLVRWDIVXRPKTIGGLGLGNISXRNLALLGKWLWRYPREGSALWHQAIAQVFQEF 1486

Query: 984  YL---------------GELPTKGKFSSSKAPRMF-IIKRVDWVLPQDLCPRRPLR---- 1043
             L                +L    +   ++ PR+F ++   +  +   L P RP      
Sbjct: 1487 SLITRYVVGNGDRIRFWEDLWRGDQXLGTQYPRLFRVVVDKNISISSVLXPSRPFLWNLN 1546

Query: 1044 -------SVEEALWGNMKAS-----LPSLPAFGFDIPRWNLNNNSSFTVASIKLARPLNN 1052
                   S  E L G M++       PS+P    D   W L+++  F+V S  LA   ++
Sbjct: 1547 FRRNLSDSEIEDLEGLMRSLDDLYLSPSVP----DARLWPLSSSGLFSVKSFFLALSQSS 1606

BLAST of Cucsa.240430.1 vs. NCBI nr
Match: gi|657990185|ref|XP_008387315.1| (PREDICTED: uncharacterized protein LOC103449768 [Malus domestica])

HSP 1 Score: 424.5 bits (1090), Expect = 5.5e-115
Identity = 248/735 (33.74%), Postives = 377/735 (51.29%), Query Frame = 1

Query: 124  DREAISEVNVMNFSISVKWHFSPAKSSSGGIVIMWDDIRFKVVDYLVKKFTLSIEITYPN 183
            DR  ++ V    F    +W ++PA+ SSGGI ++W+     V + L+  F++SI+I    
Sbjct: 1042 DRRLVASVWGSRFK---EWIYAPAQGSSGGIAVIWNTKNISVTESLIGVFSVSIKI---K 1101

Query: 184  DSNNLNWWLTVVCGPSKRENRGDFWIELHEIKSICLPRWLMAKSITF------------- 243
              N L WWL+ V GP K   R +FW E+  +  +C P+W +                   
Sbjct: 1102 AFNGLEWWLSGVYGPCKSRERREFWEEMAGLYGLCGPKWCVGGDFNVVRFVNEKSNGGRL 1161

Query: 244  -SCMNNFNTFISGCDFIDPPLTNAKFTWSNLRAQPILSRLNIFLYTPNWEILFEPHFSKM 303
             + M NFN FI   +  D  L NA+FTWSN R +P+  RL+ FL +   E +F       
Sbjct: 1162 TTSMRNFNDFIRETELKDLELLNAQFTWSNFREEPVCRRLDRFLVSAGCEEIFPEVRQMA 1221

Query: 304  LPRVTSDHFSIALESNSLKWGCSPFIFTNSFLKEVSFKQNIEVWWKNTAQVGHPGYSFMG 363
            L RV SDH  I LESN +KWG SPF F N +L+   F+    +WW++    G  GY FM 
Sbjct: 1222 LARVISDHCPIQLESNKVKWGPSPFRFENMWLQHPEFRNKFNLWWQSEQVEGWEGYKFMI 1281

Query: 364  RLKQLSYTIKNWSKSLTKSNEEEIQTLLNELEHIDNLEVDSNITNLHITRRASIKTDLCQ 423
            +LK     ++ WSK      E++ +     LE +D  E    +      +R  +      
Sbjct: 1282 KLKAXKKKVQRWSKESFGEVEKDFKEAEASLEELDRREGMEGLDVDARRKREELLFXXGD 1341

Query: 424  MAFKEAQIWAKKCKRLWNLEGDENYAFYHKICSARQRRSFISSISTA------------- 483
            +A+KE   W ++ K  W  EGD N  F+H++ S R++R++I  +                
Sbjct: 1342 LAYKEEVKWRQRSKVEWXKEGDGNTKFFHRVASGRRKRNYIERLEXEVGGVIEDANEIED 1401

Query: 484  ------QGVLCSSD-----------IPIKTTTHKDLCKMFYEEEIYNALNTFSKNKSSGP 543
                  + +  S++            PI       + + F E E+  A+    K+KS GP
Sbjct: 1402 HIVXFFKSLFSSNEEACWGLEGINWAPISELEANWIERPFEEAEVQRAVFDCGKDKSPGP 1461

Query: 544  DGFTTKFLKGTWKFTKENILKIFNDLHKNGIINKIEK------------SYVLLDYRPIS 603
            DG++ + ++  W   K NI+KI  + ++ GIIN +              S  + D+RPIS
Sbjct: 1462 DGYSLQMIQQCWDILKANIMKIMEEFYETGIINAVTNETFICLIPKKSDSMKVTDFRPIS 1521

Query: 604  LTTGLYKLIAKVIAERLKLVLPEIISENQLAF-RGRQITDAILIASEAVDFWKKKKVKGF 663
            L TGLYK++AK +A RLK VL   IS+NQ AF + RQI DA+LIA+E V+  ++KK +G 
Sbjct: 1522 LVTGLYKIMAKTLASRLKEVLGSTISQNQGAFVKDRQILDAVLIANEVVEEVRQKKEEGL 1581

Query: 664  VVKLDIEKTFDKINWKFMDFMLLKKDFPQKWRKWIEAYL--------------GKIKPNR 723
            V+K+D EK +D + W+F+D +L +K F  +WRKW++  L              GK + +R
Sbjct: 1582 VLKIDFEKAYDHVEWRFLDEVLQRKSFGNRWRKWMQGCLSSANFSVLINGRPRGKFQXSR 1641

Query: 724  GIRQGDHISPFIFVLAMDYLSTLLNHMEKQNHIKGVSF-NGKHNLTHILFAYDILLFLED 783
            G+RQGD +SPF+F L +D LS L+   ++ + IKG+     K  + H+ FA D + FL  
Sbjct: 1642 GLRQGDPLSPFLFTLVVDVLSRLMEKAQENHLIKGLCIGQEKVEIXHLQFADDTIFFLAX 1701

Query: 784  DDKTIDNMRFSFRLFELASGLNINLNKSTISPINTDAQRTNCVAKKWGISINFLPIQYLG 787
             +   +N+    +LF   SGL IN  K  +  IN+D ++ N +A  WG  +   PI+YLG
Sbjct: 1702 XEGGWNNLLELLKLFCSVSGLKINKAKCYLXGINSDCEKLNRLADSWGCEVGSXPIKYLG 1761

BLAST of Cucsa.240430.1 vs. NCBI nr
Match: gi|657990185|ref|XP_008387315.1| (PREDICTED: uncharacterized protein LOC103449768 [Malus domestica])

HSP 1 Score: 74.7 bits (182), Expect = 1.1e-09
Identity = 42/130 (32.31%), Postives = 63/130 (48.46%), Query Frame = 1

Query: 906  VEEALWGNMKASLPSLPAFGFDIPRWNLNNNSSFTVASIKLARPLNNQAEVNYNDGVIYN 965
            +EEA     K     L     D  RW +  +  FT  S      L+N   + Y     ++
Sbjct: 1994 IEEAARLLQKVEEVRLSQSRXDNRRWKMEASGLFTCKSY--CSFLSNNGMMQYFQP--HS 2053

Query: 966  NVWKSSIPKMCKLFLWTLIQECINTTDMLQRRLPTWNIKPSWCTLCKAAEEDRNHLFSLY 1025
             +WKS +P   K+ +W   +  +NT D +QRR P   + P WC+LCKA EE  NH+F   
Sbjct: 2054 QIWKSKVPPKVKILVWLAAKGKLNTCDQIQRRSPFICLSPQWCSLCKAKEESVNHIFLHC 2113

Query: 1026 PFSSKLWQKV 1036
             ++ +LW K+
Sbjct: 2114 SYTIQLWWKL 2119

HSP 2 Score: 422.2 bits (1084), Expect = 2.7e-114
Identity = 302/1051 (28.73%), Postives = 492/1051 (46.81%), Query Frame = 1

Query: 98   KKKLMAWLKNNNPKLTTNSFVDIENEDREAISEVNVMNFSISVKWHFSPAKSSSGGIVIM 157
            ++ +  +L++ NP +        EN DR  +  V  +    +  W   P   +SGGI+I+
Sbjct: 17   RRMVKDFLRSENPDVVMIQETKKENCDRRFVGSVWTVR---NKDWVALPVSGASGGILII 76

Query: 158  WDDIRFKVVDYLVKKFTLSIEITYPNDSNNLNWWLTVVCGPSKRENRGDFWIELHEIKSI 217
            WD    +  + ++  F++S++ +          W++ V GP+    R DFW+EL +I  +
Sbjct: 77   WDSKNLRREEVVIGSFSISVKFSLDGCGP---LWISAVYGPNSPSLRKDFWVELFDIYGL 136

Query: 218  CLPRW---------------LMAKSITFSCMNNFNTFISGCDFIDPPLTNAKFTWSNLRA 277
                W               L   S+T S M +F++FI  C+ +DPPL NA FTWSN++ 
Sbjct: 137  TYLLWCVGGDFNVIRRSSEKLGGSSLTPS-MRDFDSFIRECELLDPPLRNASFTWSNMQE 196

Query: 278  QPILSRLNIFLYTPNWEILFEPHFSKMLPRVTSDHFSIALESNSLKWGCSPFIFTNSFLK 337
             P+  RL+ FLY+  W +LF     + L R TSDH+ I +++N  +WG +PF F N +L+
Sbjct: 197  SPVCKRLDRFLYSNEWGLLFPQGLQEALIRRTSDHWPIVMDTNPFRWGPTPFRFENMWLQ 256

Query: 338  EVSFKQNIEVWWKNTAQVGHPGYSFMGRLKQLSYTIKNWSKSLTKSNEEEIQTLLNELEH 397
              +FK+N   WW      G  G+ FM RL+ +   +K W+K      +E+  ++LN+L +
Sbjct: 257  HTNFKENFRDWWSGFQGNGWEGHKFMRRLQYVKAKLKEWNKFSFGELKEKKNSILNDLAN 316

Query: 398  IDNLEVDSNITNLHITRRASIKTDLCQMAFKEAQIWAKKCKRLWNLEGDENYAFYHKICS 457
             D +E +  +  + ++ RAS K +L ++  +E   W +K K  W  EGD N  FYHK+ +
Sbjct: 317  FDAIEQEGGLNPVLLSXRASRKGELEELILREEIHWRQKAKVKWVKEGDCNSKFYHKVAN 376

Query: 458  ARQRRSF------------------------------ISSISTAQGVLCSSDIPIKTTTH 517
             ++ R +                               +    + GV       I   + 
Sbjct: 377  GKRNRKYJKELENERGLVLKNAESITXEILHYFEKLYTNPTGESWGVEGLDWSLISEESA 436

Query: 518  KDLCKMFYEEEIYNALNTFSKNKSSGPDGFTTKFLKGTWKFTKENILKIFNDLHKNGIIN 577
              L   F EEEI  A+    ++K+ GPDGFT    +  W   KE+++++F + H++GIIN
Sbjct: 437  LRLDSPFTEEEISKAIFQLDRDKALGPDGFTIAVFQECWDVIKEDLVRVFAEFHRSGIIN 496

Query: 578  K-IEKSYVLL-----------DYRPISLTTGLYKLIAKVIAERLKLVLPEIISENQLAF- 637
            +    S+++L           D+RPISL T LYK+IAKV+  RL+ VL E I   Q AF 
Sbjct: 497  QSTNASFIVLIPKKCLSKRISDFRPISLITSLYKIIAKVLFGRLRGVLHETIHYTQGAFV 556

Query: 638  RGRQITDAILIASEAVDFWKKKKVKGFVVKLDIEKTFDKINWKFMDFMLLKKDFPQKWRK 697
            +GRQI  A+LIA+E VD  ++   +G V K+D EK +D + W F+D +L KK F  +WRK
Sbjct: 557  QGRQILHAVLIANEIVDERRRSGEEGVVFKIDFEKAYDHVKWDFLDHVLEKKGFSPRWRK 616

Query: 698  WIEAYL--------------GKIKPNRGIRQGDHISPFIFVLAMDYLSTLLNHMEKQNHI 757
            W+   L              G +K +RG+RQGD +SPF+F L  D LS +L   E++N +
Sbjct: 617  WMSGCLSSVSYAILVNGSAKGWVKASRGLRQGDPLSPFLFTLVADVLSRMLMRAEERNMM 676

Query: 758  KGVSFN-GKHNLTHILFAYDILLFLEDDDKTIDNMRFSFRLFELASGLNINLNKSTISPI 817
            +G      K  ++H+ FA D + F    ++ +  ++    +F   SGL +NLNKS+I  I
Sbjct: 677  EGFRVGRNKTRVSHLQFADDTIFFSNSREEELQTLKSLLLVFGHISGLKVNLNKSSIYGI 736

Query: 818  ------------NTDAQRTNCVAKKWGISINFLP---------IQYLGVPLGG-KPSSRN 877
                          D + +        + +   P         ++ +   L G +    +
Sbjct: 737  NLDQAHLSRLAEMLDCKASGWPILYLSLPLGGNPKACGFWDPVVERISSRLDGWQKPYLS 796

Query: 878  FG------------IPNYHLSVLKPLKSVYNDIEKIWRNFLWRNTYEKKNINLIKWSTIL 937
            FG            +P+Y LS+ K    V   IE+I R+FLW   + ++  +  +    +
Sbjct: 797  FGGRITLIQSCLTHLPSYFLSLFKIPALVAAKIERIQRDFLWSRGWGRQKGSFSEVGCCV 856

Query: 938  SPHKGGLGINSVENFMRKKNPLLKCIIIAKYEQSYLGELPTKGKFSSSKAPRMFIIKRVD 997
               K   G    E F+ +     + ++  +  +  + +  +         P ++      
Sbjct: 857  QT-KDNRGFGFGEYFLEESRSSREMVMEPRLFRVVVDKNISISSVLGPSRPFLW------ 916

Query: 998  WVLPQDLCPRRPLRSVE-EALWGNMKAS-----LPSLPAFGFDIPRWNLNNNSSFTVASI 1036
                 +L  RR L   E E L G M++       PS+P    D   W L+++  F+V S 
Sbjct: 917  -----NLNFRRNLSDSEIEDLEGLMRSLDDLYLSPSVP----DARLWPLSSSGLFSVKSF 976

BLAST of Cucsa.240430.1 vs. NCBI nr
Match: gi|147859788|emb|CAN83561.1| (hypothetical protein VITISV_024106 [Vitis vinifera])

HSP 1 Score: 417.9 bits (1073), Expect = 5.2e-113
Identity = 252/782 (32.23%), Postives = 397/782 (50.77%), Query Frame = 1

Query: 121  ENEDREAISEVNVMNFSISVKWHFSPAKSSSGGIVIMWDDIRFKVVDYLVKKFTLSIEIT 180
            EN DR  +  V  +    +  W   PA  +SGGI+I+WD       + ++  F++S++ +
Sbjct: 675  ENCDRRFVGSVWTVR---NKDWVALPASGASGGILIIWDSKILSREEVVIGSFSVSVKFS 734

Query: 181  YPNDSNNLNWWLTVVCGPSKRENRGDFWIELHEIKSICLPRWL--------------MAK 240
                      W++ V GP+    R DFW+EL +I  +  P W               M  
Sbjct: 735  LDGCGP---LWISAVYGPNSPSLRKDFWVELFDIYGLTYPLWCVGGDFNVIRRSSEKMGG 794

Query: 241  SITFSCMNNFNTFISGCDFIDPPLTNAKFTWSNLRAQPILSRLNIFLYTPNWEILFEPHF 300
            S     M +F++FIS C+ +DPPL NA FTWSN++  P+  RL+ FLY+  W +LF    
Sbjct: 795  SSLTPSMRDFDSFISECELLDPPLRNASFTWSNMQESPVCKRLDRFLYSNEWGLLFPQGL 854

Query: 301  SKMLPRVTSDHFSIALESNSLKWGCSPFIFTNSFLKEVSFKQNIEVWWKNTAQVGHPGYS 360
             + L R TSDH+ I +++N   WG +PF F N +L+  +FK+N   WW     +G  G+ 
Sbjct: 855  QEALIRRTSDHWPIVMDTNPFMWGPTPFRFENMWLQHTNFKENFRDWWSGFQGIGWEGHK 914

Query: 361  FMGRLKQLSYTIKNWSKSLTKSNEEEIQTLLNELEHIDNLEVDSNITNLHITRRASIKTD 420
            FM RL+ +   +K W+KS     +E+ +++LN+L + D +E +  +    I++R S K +
Sbjct: 915  FMRRLQYVKAKLKEWNKSSFGELKEKKKSILNDLANFDAIEQEGGLNPDLISQRVSRKGE 974

Query: 421  LCQMAFKEAQIWAKKCKRLWNLEGDENYAFYHKICSARQRRSFISSISTAQGVLCSSD-- 480
            L ++  +E   W +K K  W  EGD N  FYHK+ + R+ R +I  +   +G++  +   
Sbjct: 975  LEELILREEIHWRQKAKVKWVKEGDCNSKFYHKVANGRRNRKYIKELENERGLVLKNAER 1034

Query: 481  ----------IPIKTTTHKDLCKMFYEEEIYNALNTFSKNKSSGPDGFTTKFLKGTWKFT 540
                       PI   +   L   F EEEI  A+    ++K+ GPDGFT    +  W   
Sbjct: 1035 ESWGVEGLDWSPISEESALRLESPFTEEEISKAIFQLDRBKAPGPDGFTIAVFQECWDVI 1094

Query: 541  KENILKIFNDLHKNGIINK-IEKSYVLL-----------DYRPISLTTGLYKLIAKVIAE 600
            KE+++++F + H++GIIN+    S+++L           D+RPISL T LYK+IAKV++ 
Sbjct: 1095 KEDLVRVFAEFHRSGIINQSTNASFIVLIPKKSLSKRISDFRPISLITSLYKIIAKVLSG 1154

Query: 601  RLKLVLPEIISENQLAFRGRQITDAILIASEAVDFWKKKKVKGFVVKLDIEKTFDKINWK 660
            RL+               G +  +A+LIA+E VD  ++   +G V K+D EK +D + W 
Sbjct: 1155 RLR--------------GGDKYWNAVLIANEIVDERRRSXEEGVVFKIDFEKAYDHVKWD 1214

Query: 661  FMDFMLLKKDFPQKWRKWIEAYL--------------GKIKPNRGIRQGDHISPFIFVLA 720
            F+D +L KK F  +WRKW+   L              G +K +RG+RQGD +SPF+F L 
Sbjct: 1215 FLDHVLEKKGFSPRWRKWMSGCLSSVSFAILVNGSAKGWVKASRGLRQGDPLSPFLFTLV 1274

Query: 721  MDYLSTLLNHMEKQNHIKGVSF-NGKHNLTHILFAYDILLFLEDDDKTIDNMRFSFRLFE 780
             D LS +L   E++N ++G      +  ++H+ FA D + F    ++ +  ++    +F 
Sbjct: 1275 ADVLSRMLMRAEERNMLEGFRVGRNRTRVSHLQFADDTIFFSNSREEELQTLKSLLLVFG 1334

Query: 781  LASGLNINLNKSTISPINTDAQRTNCVAKKWGISINFLPIQYLGVPLGGKPSSRNFGIPN 840
              SGL +NLNKS+I  IN D    + +A+      +  PI YLG+PLGG P +  F  P 
Sbjct: 1335 HISGLKVNLNKSSIYGINLDQAHLSRLAEMLDCKASGWPILYLGLPLGGNPKACGFWDPV 1394

Query: 841  YHLSVLKPLKSVYNDIEKI-WRN------FLWRNTYEKKNINLIKWSTILSPHKGGLGIN 843
                + KP       +  I WRN      +LWR   E   +      +I   H  G   N
Sbjct: 1395 VE-RISKPKTIGGLGLGNISWRNLALLGKWLWRYPREGSXLWHQVILSIYGSHSNGWDAN 1435

BLAST of Cucsa.240430.1 vs. NCBI nr
Match: gi|147859788|emb|CAN83561.1| (hypothetical protein VITISV_024106 [Vitis vinifera])

HSP 1 Score: 62.0 bits (149), Expect = 7.2e-06
Identity = 42/139 (30.22%), Postives = 64/139 (46.04%), Query Frame = 1

Query: 919  PSLPAFGFDIPRWNLNNNSSFTVASIKLARPLNNQAEVNYNDGVIYNNVWKSSIPKMCKL 978
            PS+P    D   W L+++  F+V S  LA   ++ +  N+        VW S +P   K 
Sbjct: 1541 PSVP----DARLWPLSSSGLFSVKSFFLALSQSSGSSQNFPSKF----VWNSQVPFKVKS 1600

Query: 979  FLWTLIQECINTTDMLQRRLPTWNIKPSWCTLCKAAEEDRNHLFSLYPFSSKLWQ----- 1038
            F+W +  + +NT DMLQ R P   + P  C LC    E  +HLF     +  LW      
Sbjct: 1601 FVWLVAHKKVNTNDMLQVRRPYKALSPDICILCMKHGESXDHLFLHCSLTIGLWHRLFQL 1660

Query: 1039 -KVEVILDRPLYPINSPLF 1052
             K++ +  R +Y + S  F
Sbjct: 1661 AKLDWVSPRSIYDMMSIKF 1671

HSP 2 Score: 413.7 bits (1062), Expect = 9.8e-112
Identity = 244/705 (34.61%), Postives = 369/705 (52.34%), Query Frame = 1

Query: 124  DREAISEVNVMNFSISVKWHFSPAKSSSGGIVIMWDDIRFKVVDYLVKKFTLSIEITYPN 183
            DR+ ++ V    F    +W FSP+   SGGI ++W+     V+D +V +F++SI I    
Sbjct: 365  DRQLVAGVWGSRFK---EWVFSPSLGRSGGIAVLWNSQSVSVIDSMVGEFSVSIRI---E 424

Query: 184  DSNNLNWWLTVVCGPSKRENRGDFWIELHEIKSICLPRWLMAKS---ITFSC-------- 243
            ++   +WWL+ + GP ++  R  FW EL ++   C   W +      + FS         
Sbjct: 425  ENIGTDWWLSGIYGPCRQRERNSFWEELADLYGYCGDMWCLGGDFNVVRFSAEKSNEGRV 484

Query: 244  ---MNNFNTFISGCDFIDPPLTNAKFTWSNLRAQPILSRLNIFLYTPNWEILFEPHFSKM 303
               M +FN FI   +  DP L NA FTWSNLR   +  RL+ FL + +WE  F  +  K 
Sbjct: 485  TKSMRDFNDFIQETNLRDPILLNASFTWSNLRENAVCRRLDRFLVSGSWEEHFPHYRHKA 544

Query: 304  LPRVTSDHFSIALESNSLKWGCSPFIFTNSFLKEVSFKQNIEVWWKNTAQVGHPGYSFMG 363
            LPR+TSDH  I L+++ +KWG SPF F N +L    FK+ I++WW      G  GY FM 
Sbjct: 545  LPRITSDHCPIELDTSRVKWGPSPFRFENMWLNHPDFKRKIKLWWGEDQIPGWEGYKFMT 604

Query: 364  RLKQLSYTIKNWSKSLTKSNEEEIQTLLNELEHIDNLEVDSNITNLHITRRASIKTDLCQ 423
            RLK L   +K WSK      E +++     L  +D  E    + +L  + R ++   +  
Sbjct: 605  RLKMLKSKLKVWSKEEFGDVERDLREAEARLLVLDQREGTEGLDHLLRSERDNLLLKIGD 664

Query: 424  MAFKEAQIWAKKCKRLWNLEGDENYAFYHKICSARQRRSFISSISTAQGVLCSSDIPIKT 483
            +A KE   W ++ K  W  +GD N  F+H++ +  ++R++I  +      +   D  I+ 
Sbjct: 665  LAQKEEVKWRQRGKVKWARDGDGNTKFFHRVANGARKRNYIEKLEVEDLGVIEVDANIER 724

Query: 484  TTHKDLCKMFYEEEIYNALNTFSKNKSSGPDGFTTKFLKGTWKFTKENILKIFNDLHKNG 543
               +   K  Y      A+    K+KS GPDGF+  F +  W+  K +++K+  D  ++G
Sbjct: 725  EVIR-FFKGLYSSNKNKAVFDCGKDKSPGPDGFSMSFFQSCWEVVKGDLMKVMQDFFQSG 784

Query: 544  IIN------------KIEKSYVLLDYRPISLTTGLYKLIAKVIAERLKLVLPEIISENQL 603
            I+N            K   S  + DYRPISL T LYK+I+KV+A  L+ VL   IS++Q 
Sbjct: 785  IVNGVTNETFICLIPKKANSVKVTDYRPISLVTSLYKVISKVLASSLREVLGNTISQSQG 844

Query: 604  AF-RGRQITDAILIASEAVDFWKKKKVKGFVVKLDIEKTFDKINWKFMDFMLLKKDFPQK 663
            AF + RQI DA+L+A+E V+  +K+K KG V K+D EK +D + W F+D ++ +K F  K
Sbjct: 845  AFVQKRQILDAVLVANEVVEEVRKQKRKGLVFKIDFEKAYDHVEWNFVDDVMARKGFGVK 904

Query: 664  WRKWIEAYL--------------GKIKPNRGIRQGDHISPFIFVLAMDYLSTLLNHMEKQ 723
            WR WI   L              GK + +RG+RQGD +SPF+F L  D LS L+   +  
Sbjct: 905  WRGWIIGCLESVNFSIMINGKPRGKFRASRGLRQGDPLSPFLFTLVSDVLSRLIERAQDV 964

Query: 724  NHIKG-VSFNGKHNLTHILFAYDILLFLEDDDKTIDNMRFSFRLFELASGLNINLNKSTI 783
            N + G VS + +  ++H+ FA D +  L+  ++   N+    +LF   SG+ IN  KS I
Sbjct: 965  NLVHGIVSGHDQVEVSHLQFADDTIFLLDGKEEYWLNLLQLLKLFCDVSGMKINKAKSCI 1024

Query: 784  SPINTDAQRTNCVAKKWGISINFLPIQYLGVPLGGKPSSRNFGIP 787
              IN      N +A  WG  +   P+ YLG+PLGG P + NF  P
Sbjct: 1025 LGINFSTDVLNNMAGSWGCEVGCWPMVYLGLPLGGNPRALNFWNP 1062