Cla010567.1 (mRNA) Watermelon (97103) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cla010567
Type	mRNA
Organism	Citrullus. lanatus (Watermelon (97103) v1)
Description	Methionyl-tRNA synthetase (AHRD V1 ***- E9BX03_CAPO3); contains Interpro domain(s) IPR015413 Aminoacyl-tRNA synthetase, class I (M)
Location	Chr6 : 10257046 .. 10258897 (+)
Sequence length	648

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAGACGTTTCAGGCGCCGCCGCCGGAACCAAGACCACAAAGCTTCCTATTCCAGGAAAGAGGAACATTCTCATCACCAGCGCTCTTCCATATGTCAACAACGTTCCTCACCTTGGCAACATCATTGGCTGTAATGTTCTTTCTCACTTGTGTATATATTATATGTAATATTTATGTTTCAGTGTGTTTGATTGATTGAGGATTCTTTTTTTCTTCTCGTTTCTGTGTGCTCGTGTTTGAATTGGTTGCTGTTCGGTTATTGAGAATCGAATAATGGAGATTTGAGGAATTATGCTGCCTTCTCAGTTTTACTTCTAGTGCGCCTCATTTTTGTGATGCGGATTGTTGTTATGATTATTAGAAGGCTCAATGAAATGTTTTGTTTATATTTGATTTCGATATTCTGTTATACTTCCACCATGCGGATGGATTGATTTTTTTGTGTATGCAGGTGTGTTGAGTGCTGATGTTTTTGCTCGATACTGTCGTCTTAGAGGTTATAACGCTATTTACATTTGCGGAACCGATGAGTATGGAACGGCGACGGAGACAAAAGCAATGGAAGAGAAATGCTCTCCTAAAGAGATTTGTGACAAGTAAGTTTTCTTCTCTGGTTTCTGTTAGTCGTGGTTACTTCATCTGTGATTCTGGAAAATAGTTCGATCGGCTTTCTGTAGCTCGTTTTGTATATAATCCCTACTCACGTAATGTTCCAATTTCAATTTCTCTTCTATCTTCCACGAGTATAGTTTTGTAAATCAGATGAAGATCTGGGTCTCATGCTTTTGGTACTGTTTTCTGGACTCGGATACTGTCATAACTACTTCTCGGACATTTTTTTCGTATTAAAATTTTGTTGAACGGTTCTGATTTTCGCATGCATGTAATTTCTCGGAGCATTTTTTAACATAATCTCTTCCTTACTTCCGTAATGGATTAATTTGGTCGAATTTTATGTACTTGTACACTTAATTGACCGTTCTGATTGAATTTATATTTTTGATGCTCTGCAATCTCTCTCTTCCTCTCTTGAATCCCAGTTCCCTCTACTTGCTTAGACGTCACCTATATCTGGGGAAAGTTTTGGACCTTTATTTAACTATTTCTGCATGTCAGGTATCACGCGATTCACAAAGAAGTTTATAATTGGTTTGGTATTAGTTTTGATGAGTTTGGCCGCACATCATCGCCACAGCAGACTGAAGTTTGTCAAGCAATATTTGGCAAACTATTGAAGAACAATTGGCTTTCTGAGAACACGATGCAACAGGTAGAGTTATTTATCACTTGAGTATTATATTCATCTCCTGGTTACTTATGGTTGCATGTGCTTGAGTTCTTCTACATCTGTTTACTCATGCATGATATTTATTTAGTTTCTTTTACCCATACTTCAGCTGTACTGTGATACTTGTGAGAGATTTTTAGCCGATCGGCTCGTTGAAGGCATATGTCCTACTCCAGGTTGTGAGTATGACTCCGCACGTGGTGATCAGTGTGAGAAGTGCGGGAAACTTCTAAATCCAACTGAATTGAAAGACCCTAGGTGCAAGGTACATCATGGATTTACTGCAACTGATAAACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTGCATTATAGAATCTTTCATGAAATGCCGCGAACTCTATAGAACTTCCTAGGACATTGTGCAAACATATTCCAACTGGAAGAGAGTTTTTTTCCCCTCCTTTTTATTGGGTGGGGAGGGGGGAGAATCAATATACTCTCAAATTTCTTCTTGATAGTTGATCCCATCCTGAAGGACTCTTCTCCTTTCAAATCAAACAACACCATTCGCCTCTTAACTCTCGACAAAAGGTAG

mRNA sequence

ATGGGAGACGTTTCAGGCGCCGCCGCCGGAACCAAGACCACAAAGCTTCCTATTCCAGGAAAGAGGAACATTCTCATCACCAGCGCTCTTCCATATGTCAACAACGTTCCTCACCTTGGCAACATCATTGGCTGTGTGTTGAGTGCTGATGTTTTTGCTCGATACTGTCGTCTTAGAGGTTATAACGCTATTTACATTTGCGGAACCGATGAGTATGGAACGGCGACGGAGACAAAAGCAATGGAAGAGAAATGCTCTCCTAAAGAGATTTGTGACAAGTATCACGCGATTCACAAAGAAGTTTATAATTGGTTTGGTATTAGTTTTGATGAGTTTGGCCGCACATCATCGCCACAGCAGACTGAAGTTTGTCAAGCAATATTTGGCAAACTATTGAAGAACAATTGGCTTTCTGAGAACACGATGCAACAGCTGTACTGTGATACTTGTGAGAGATTTTTAGCCGATCGGCTCGTTGAAGGCATATGTCCTACTCCAGGTTGTGAGTATGACTCCGCACGTGGTGATCAGTGTGAGAAGTGCGGGAAACTTCTAAATCCAACTGAATTGAAAGACCCTAGGTGCAAGGACTCTTCTCCTTTCAAATCAAACAACACCATTCGCCTCTTAACTCTCGACAAAAGGTAG

Coding sequence (CDS)

ATGGGAGACGTTTCAGGCGCCGCCGCCGGAACCAAGACCACAAAGCTTCCTATTCCAGGAAAGAGGAACATTCTCATCACCAGCGCTCTTCCATATGTCAACAACGTTCCTCACCTTGGCAACATCATTGGCTGTGTGTTGAGTGCTGATGTTTTTGCTCGATACTGTCGTCTTAGAGGTTATAACGCTATTTACATTTGCGGAACCGATGAGTATGGAACGGCGACGGAGACAAAAGCAATGGAAGAGAAATGCTCTCCTAAAGAGATTTGTGACAAGTATCACGCGATTCACAAAGAAGTTTATAATTGGTTTGGTATTAGTTTTGATGAGTTTGGCCGCACATCATCGCCACAGCAGACTGAAGTTTGTCAAGCAATATTTGGCAAACTATTGAAGAACAATTGGCTTTCTGAGAACACGATGCAACAGCTGTACTGTGATACTTGTGAGAGATTTTTAGCCGATCGGCTCGTTGAAGGCATATGTCCTACTCCAGGTTGTGAGTATGACTCCGCACGTGGTGATCAGTGTGAGAAGTGCGGGAAACTTCTAAATCCAACTGAATTGAAAGACCCTAGGTGCAAGGACTCTTCTCCTTTCAAATCAAACAACACCATTCGCCTCTTAACTCTCGACAAAAGGTAG

Protein sequence

MGDVSGAAAGTKTTKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRLRGYNAIYICGTDEYGTATETKAMEEKCSPKEICDKYHAIHKEVYNWFGISFDEFGRTSSPQQTEVCQAIFGKLLKNNWLSENTMQQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQCEKCGKLLNPTELKDPRCKDSSPFKSNNTIRLLTLDKR

Homology

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BLAST of Cla010567 vs. Swiss-Prot
Match: SYMC_ARATH (Methionine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana GN=At4g13780 PE=2 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 1.6e-98
Identity = 164/187 (87.70%), Postives = 173/187 (92.51%), Query Frame = 1


Query: 10  GTKTTKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRLRGYNAIYICGT 69
           G  + KLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADV+ARYCRLRGYNAIYICGT
Sbjct: 5   GKSSPKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVYARYCRLRGYNAIYICGT 64

Query: 70  DEYGTATETKAMEEKCSPKEICDKYHAIHKEVYNWFGISFDEFGRTSSPQQTEVCQAIFG 129
           DEYGTATETKA+EE C+PKEICDKYHAIHKEVY+WFGISFD+FGRTS+P+QTEVCQAIF 
Sbjct: 65  DEYGTATETKALEENCTPKEICDKYHAIHKEVYDWFGISFDKFGRTSTPEQTEVCQAIFN 124

Query: 130 KLLKNNWLSENTMQQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQCEKCGKLLNPTE 189
           KL  N WLSENTMQQLYCDTC++FLADRLVEG CP  GC YDSARGDQCEKCGKLLNPTE
Sbjct: 125 KLWDNKWLSENTMQQLYCDTCKKFLADRLVEGSCPFEGCNYDSARGDQCEKCGKLLNPTE 184

Query: 190 LKDPRCK 197
           LKDP+CK
Sbjct: 185 LKDPKCK 191

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BLAST of Cla010567 vs. Swiss-Prot
Match: SYM_ORYSJ (Probable methionine--tRNA ligase OS=Oryza sativa subsp. japonica GN=Os06g0508700 PE=2 SV=2)

HSP 1 Score: 352.1 bits (902), Expect = 4.4e-96
Identity = 158/182 (86.81%), Postives = 168/182 (92.31%), Query Frame = 1


Query: 15  KLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRLRGYNAIYICGTDEYGT 74
           KLP+PG+RNIL+TSALPYVNNVPHLGNIIGCVLSADVFARYCRLRGYN IYICGTDEYGT
Sbjct: 9   KLPVPGRRNILVTSALPYVNNVPHLGNIIGCVLSADVFARYCRLRGYNVIYICGTDEYGT 68

Query: 75  ATETKAMEEKCSPKEICDKYHAIHKEVYNWFGISFDEFGRTSSPQQTEVCQAIFGKLLKN 134
           ATETKAMEEKCSPKEICDKYHA+H EVY WF I FD+FGRTSSPQQTEVCQAIF KL++N
Sbjct: 69  ATETKAMEEKCSPKEICDKYHAVHSEVYKWFDIKFDKFGRTSSPQQTEVCQAIFQKLMEN 128

Query: 135 NWLSENTMQQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQCEKCGKLLNPTELKDPR 194
           NWL+ENTMQQLYCDTC+RFLADRLVEG CPT GC Y++ARGDQCE C KLLNPTEL DP+
Sbjct: 129 NWLTENTMQQLYCDTCQRFLADRLVEGKCPTEGCNYEAARGDQCENCSKLLNPTELIDPK 188

Query: 195 CK 197
           CK
Sbjct: 189 CK 190

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BLAST of Cla010567 vs. Swiss-Prot
Match: SYMC_MOUSE (Methionine--tRNA ligase, cytoplasmic OS=Mus musculus GN=Mars PE=1 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 8.7e-76
Identity = 127/181 (70.17%), Postives = 152/181 (83.98%), Query Frame = 1


Query: 16  LPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRLRGYNAIYICGTDEYGTA 75
           LP+PG+RN+LITSALPYVNNVPHLGNIIGCVLSADVFARYCRLR +N +Y+CGTDEYGTA
Sbjct: 260 LPVPGERNVLITSALPYVNNVPHLGNIIGCVLSADVFARYCRLRQWNTLYLCGTDEYGTA 319

Query: 76  TETKAMEEKCSPKEICDKYHAIHKEVYNWFGISFDEFGRTSSPQQTEVCQAIFGKLLKNN 135
           TETKAMEE  +P+EICDKYHAIH ++Y WFGISFD FGRT++PQQT++ Q IF +LL   
Sbjct: 320 TETKAMEEGLTPREICDKYHAIHADIYRWFGISFDTFGRTTTPQQTKITQDIFQRLLTRG 379

Query: 136 WLSENTMQQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQCEKCGKLLNPTELKDPRC 195
           ++  +T++QL C+ C RFLADR VEG+CP   C Y+ ARGDQC++CGKL+N  ELK P+C
Sbjct: 380 FVLRDTVEQLRCERCARFLADRFVEGVCPF--CGYEEARGDQCDRCGKLINAIELKKPQC 438

Query: 196 K 197
           K
Sbjct: 440 K 438

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BLAST of Cla010567 vs. Swiss-Prot
Match: SYMC_DICDI (Probable methionine--tRNA ligase, cytoplasmic OS=Dictyostelium discoideum GN=metS PE=3 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 2.8e-74
Identity = 130/187 (69.52%), Postives = 149/187 (79.68%), Query Frame = 1


Query: 11  TKTTKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRLRGYNAIYICGTD 70
           + T  LP  G+RNILITSALPYVNNVPHLGNIIGCVLSADV+ARYCRL+ YN IYICGTD
Sbjct: 5   SNTPPLPKDGERNILITSALPYVNNVPHLGNIIGCVLSADVYARYCRLKNYNCIYICGTD 64

Query: 71  EYGTATETKAMEEKCSPKEICDKYHAIHKEVYNWFGISFDEFGRTSSPQQTEVCQAIFGK 130
           EYGTATETKA+ E C+PKEICDKYH IHKE+Y WF ISFD+FGRTS+  QTE+ Q IF K
Sbjct: 65  EYGTATETKALSEGCTPKEICDKYHEIHKEIYEWFNISFDKFGRTSTNSQTEIAQDIFNK 124

Query: 131 LLKNNWLSENTMQQLYCD-TCERFLADRLVEGICPTPGCEYDSARGDQCEKCGKLLNPTE 190
           +  N +     ++QLYC+ TC+ FLADR VEG C  P C+++ ARGDQC+ C KLLNPTE
Sbjct: 125 IKDNGYTLTQEIEQLYCEQTCKMFLADRFVEGTC--PHCKFEDARGDQCDGCSKLLNPTE 184

Query: 191 LKDPRCK 197
           L +PRCK
Sbjct: 185 LINPRCK 189

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BLAST of Cla010567 vs. Swiss-Prot
Match: SYMC_CAEEL (Methionine--tRNA ligase, cytoplasmic OS=Caenorhabditis elegans GN=mrs-1 PE=3 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 1.1e-73
Identity = 129/201 (64.18%), Postives = 153/201 (76.12%), Query Frame = 1


Query: 16  LPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRLRGYNAIYICGTDEYGTA 75
           LP PGKRNILIT+ALPYVNNVPHLGNIIGCVLSADVFARYC LRG+   Y+ GTDEYGTA
Sbjct: 29  LPQPGKRNILITAALPYVNNVPHLGNIIGCVLSADVFARYCNLRGHQTFYVGGTDEYGTA 88

Query: 76  TETKAMEEKCSPKEICDKYHAIHKEVYNWFGISFDEFGRTSSPQQTEVCQAIFGKLLKNN 135
           TETKA++E C+P+E+CDKYHAIHK +Y WFGI F  FGRT++  QTE+CQ +F KL KN 
Sbjct: 89  TETKALQEGCTPRELCDKYHAIHKGIYEWFGIDFSHFGRTTTDHQTEICQDMFLKLHKNG 148

Query: 136 WLSENTMQQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQCEKCGKLLNPTELKDPRC 195
           + S  ++ QLYC+ CE+FLADR V G CP   C YD ARGDQC+ CGKL+N  +LKD +C
Sbjct: 149 YTSSQSVDQLYCNQCEKFLADRFVTGTCPM--CAYDDARGDQCDGCGKLINAVDLKDAKC 208

Query: 196 K--DSSPFKSNNTIRLLTLDK 215
               ++P    +T   L+LDK
Sbjct: 209 HMCKATPEVKQSTHIFLSLDK 227

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BLAST of Cla010567 vs. TrEMBL
Match: A0A0A0LN88_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G120970 PE=3 SV=1)

HSP 1 Score: 404.1 bits (1037), Expect = 1.1e-109
Identity = 188/196 (95.92%), Postives = 191/196 (97.45%), Query Frame = 1


Query: 1   MGDVSGAAAGTKTTKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRLRG 60
           MGD SG AAG   +KLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRLRG
Sbjct: 1   MGDHSGKAAGANASKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRLRG 60

Query: 61  YNAIYICGTDEYGTATETKAMEEKCSPKEICDKYHAIHKEVYNWFGISFDEFGRTSSPQQ 120
           YNAIYICGTDEYGTATETKAMEEKCSPKEICDKYHAIHKEVYNWFGISFDEFGRTSSPQQ
Sbjct: 61  YNAIYICGTDEYGTATETKAMEEKCSPKEICDKYHAIHKEVYNWFGISFDEFGRTSSPQQ 120

Query: 121 TEVCQAIFGKLLKNNWLSENTMQQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQCEK 180
           TEVCQAIFGKLL+NNWLSENTMQQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQCEK
Sbjct: 121 TEVCQAIFGKLLENNWLSENTMQQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQCEK 180

Query: 181 CGKLLNPTELKDPRCK 197
           CGKLLNPTEL+DPRCK
Sbjct: 181 CGKLLNPTELQDPRCK 196

[Close]

BLAST of Cla010567 vs. TrEMBL
Match: A0A103YFP8_CYNCS (Aminoacyl-tRNA synthetase, class 1a, anticodon-binding OS=Cynara cardunculus var. scolymus GN=Ccrd_013373 PE=3 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 6.0e-100
Identity = 171/197 (86.80%), Postives = 181/197 (91.88%), Query Frame = 1


Query: 1   MGDVS-GAAAGTKTTKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRLR 60
           MGD+  G     K TKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRLR
Sbjct: 1   MGDIPIGGGEDRKATKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRLR 60

Query: 61  GYNAIYICGTDEYGTATETKAMEEKCSPKEICDKYHAIHKEVYNWFGISFDEFGRTSSPQ 120
           GYNAIY+CGTDEYGTATETKA+EE C+PKEICDKYHAIH++VYNWF ISFDEFGRTS+PQ
Sbjct: 61  GYNAIYVCGTDEYGTATETKALEENCTPKEICDKYHAIHRDVYNWFNISFDEFGRTSTPQ 120

Query: 121 QTEVCQAIFGKLLKNNWLSENTMQQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQCE 180
           QTEVCQAIF KL++NNWLSENTMQQLYCDTC +FLADRLVEG CPTPGC YDSARGDQCE
Sbjct: 121 QTEVCQAIFKKLMENNWLSENTMQQLYCDTCNKFLADRLVEGSCPTPGCNYDSARGDQCE 180

Query: 181 KCGKLLNPTELKDPRCK 197
           KCGKLLNPTEL  PRCK
Sbjct: 181 KCGKLLNPTELISPRCK 197

[Close]

BLAST of Cla010567 vs. TrEMBL
Match: A0A022Q3M2_ERYGU (Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a001483mg PE=3 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 7.9e-100
Identity = 174/198 (87.88%), Postives = 181/198 (91.41%), Query Frame = 1


Query: 1   MGDVSGAAAGTKTT--KLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRL 60
           MGD S AAA    T  KLPIPGKRNILITSALPYVNN+PHLGNIIGCVLSADVFARYCRL
Sbjct: 1   MGDCSAAAAAAAATSAKLPIPGKRNILITSALPYVNNIPHLGNIIGCVLSADVFARYCRL 60

Query: 61  RGYNAIYICGTDEYGTATETKAMEEKCSPKEICDKYHAIHKEVYNWFGISFDEFGRTSSP 120
           RGYNAIYICGTDEYGTATETKA+EE CSPKEICDKYHAIHKEVY WF ISFDEFGRTSSP
Sbjct: 61  RGYNAIYICGTDEYGTATETKALEEGCSPKEICDKYHAIHKEVYKWFNISFDEFGRTSSP 120

Query: 121 QQTEVCQAIFGKLLKNNWLSENTMQQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQC 180
           QQTEVCQ IF KLL NNWLSENTMQQLYCDTC++FLADRLV+G CPTPGC YDSARGDQC
Sbjct: 121 QQTEVCQDIFKKLLDNNWLSENTMQQLYCDTCDKFLADRLVDGNCPTPGCNYDSARGDQC 180

Query: 181 EKCGKLLNPTELKDPRCK 197
           EKCGKLLNPTEL +P+CK
Sbjct: 181 EKCGKLLNPTELLEPKCK 198

[Close]

BLAST of Cla010567 vs. TrEMBL
Match: K7LSI4_SOYBN (Uncharacterized protein OS=Glycine max PE=3 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 1.8e-99
Identity = 170/185 (91.89%), Postives = 176/185 (95.14%), Query Frame = 1


Query: 12  KTTKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRLRGYNAIYICGTDE 71
           K  KLP+ GKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRLRGYNAIYICGTDE
Sbjct: 6   KAPKLPVEGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRLRGYNAIYICGTDE 65

Query: 72  YGTATETKAMEEKCSPKEICDKYHAIHKEVYNWFGISFDEFGRTSSPQQTEVCQAIFGKL 131
           YGTATETKAMEE CSPKEICDKYHAIHKEVYNWF ISFDEFGRTSSP+QTEVCQAIF K+
Sbjct: 66  YGTATETKAMEENCSPKEICDKYHAIHKEVYNWFNISFDEFGRTSSPEQTEVCQAIFRKI 125

Query: 132 LKNNWLSENTMQQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQCEKCGKLLNPTELK 191
            +N WLSENTMQQLYC+TCERFLADRLVEG CPTPGCEYDSARGDQCEKCGKLLNPTELK
Sbjct: 126 FENKWLSENTMQQLYCNTCERFLADRLVEGTCPTPGCEYDSARGDQCEKCGKLLNPTELK 185

Query: 192 DPRCK 197
           +PRCK
Sbjct: 186 NPRCK 190

[Close]

BLAST of Cla010567 vs. TrEMBL
Match: K7LSI3_SOYBN (Uncharacterized protein OS=Glycine max PE=3 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 1.8e-99
Identity = 170/185 (91.89%), Postives = 176/185 (95.14%), Query Frame = 1


Query: 12  KTTKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRLRGYNAIYICGTDE 71
           K  KLP+ GKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRLRGYNAIYICGTDE
Sbjct: 6   KAPKLPVEGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRLRGYNAIYICGTDE 65

Query: 72  YGTATETKAMEEKCSPKEICDKYHAIHKEVYNWFGISFDEFGRTSSPQQTEVCQAIFGKL 131
           YGTATETKAMEE CSPKEICDKYHAIHKEVYNWF ISFDEFGRTSSP+QTEVCQAIF K+
Sbjct: 66  YGTATETKAMEENCSPKEICDKYHAIHKEVYNWFNISFDEFGRTSSPEQTEVCQAIFRKI 125

Query: 132 LKNNWLSENTMQQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQCEKCGKLLNPTELK 191
            +N WLSENTMQQLYC+TCERFLADRLVEG CPTPGCEYDSARGDQCEKCGKLLNPTELK
Sbjct: 126 FENKWLSENTMQQLYCNTCERFLADRLVEGTCPTPGCEYDSARGDQCEKCGKLLNPTELK 185

Query: 192 DPRCK 197
           +PRCK
Sbjct: 186 NPRCK 190

[Close]

BLAST of Cla010567 vs. NCBI nr
Match: gi|659114634|ref|XP_008457156.1| (PREDICTED: probable methionine--tRNA ligase [Cucumis melo])

HSP 1 Score: 408.3 bits (1048), Expect = 8.3e-111
Identity = 190/196 (96.94%), Postives = 193/196 (98.47%), Query Frame = 1


Query: 1   MGDVSGAAAGTKTTKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRLRG 60
           MGD SG AAGTK TKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRLRG
Sbjct: 1   MGDDSGKAAGTKATKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRLRG 60

Query: 61  YNAIYICGTDEYGTATETKAMEEKCSPKEICDKYHAIHKEVYNWFGISFDEFGRTSSPQQ 120
           YNAIYICGTDEYGTATETKAMEEKCSPK+ICDKYHAIHKEVYNWFGISFDEFGRTSSPQQ
Sbjct: 61  YNAIYICGTDEYGTATETKAMEEKCSPKDICDKYHAIHKEVYNWFGISFDEFGRTSSPQQ 120

Query: 121 TEVCQAIFGKLLKNNWLSENTMQQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQCEK 180
           TEVCQAIFGKLL+NNWLSENTMQQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQCEK
Sbjct: 121 TEVCQAIFGKLLENNWLSENTMQQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQCEK 180

Query: 181 CGKLLNPTELKDPRCK 197
           CGKLLNPTEL+DPRCK
Sbjct: 181 CGKLLNPTELQDPRCK 196

[Close]

BLAST of Cla010567 vs. NCBI nr
Match: gi|449442335|ref|XP_004138937.1| (PREDICTED: probable methionine--tRNA ligase [Cucumis sativus])

HSP 1 Score: 404.1 bits (1037), Expect = 1.6e-109
Identity = 188/196 (95.92%), Postives = 191/196 (97.45%), Query Frame = 1


Query: 1   MGDVSGAAAGTKTTKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRLRG 60
           MGD SG AAG   +KLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRLRG
Sbjct: 1   MGDHSGKAAGANASKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRLRG 60

Query: 61  YNAIYICGTDEYGTATETKAMEEKCSPKEICDKYHAIHKEVYNWFGISFDEFGRTSSPQQ 120
           YNAIYICGTDEYGTATETKAMEEKCSPKEICDKYHAIHKEVYNWFGISFDEFGRTSSPQQ
Sbjct: 61  YNAIYICGTDEYGTATETKAMEEKCSPKEICDKYHAIHKEVYNWFGISFDEFGRTSSPQQ 120

Query: 121 TEVCQAIFGKLLKNNWLSENTMQQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQCEK 180
           TEVCQAIFGKLL+NNWLSENTMQQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQCEK
Sbjct: 121 TEVCQAIFGKLLENNWLSENTMQQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQCEK 180

Query: 181 CGKLLNPTELKDPRCK 197
           CGKLLNPTEL+DPRCK
Sbjct: 181 CGKLLNPTELQDPRCK 196

[Close]

BLAST of Cla010567 vs. NCBI nr
Match: gi|747106523|ref|XP_011101554.1| (PREDICTED: probable methionine--tRNA ligase [Sesamum indicum])

HSP 1 Score: 374.4 bits (960), Expect = 1.3e-100
Identity = 176/196 (89.80%), Postives = 182/196 (92.86%), Query Frame = 1


Query: 1   MGDVSGAAAGTKTTKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRLRG 60
           MGD S  AA    TKLP+PGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCR+RG
Sbjct: 1   MGDSSAPAA----TKLPVPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRMRG 60

Query: 61  YNAIYICGTDEYGTATETKAMEEKCSPKEICDKYHAIHKEVYNWFGISFDEFGRTSSPQQ 120
           YNAIYICGTDEYGTATETKAMEE CSPKEICDKYHAIHK+VY WF ISFDEFGRTSSPQQ
Sbjct: 61  YNAIYICGTDEYGTATETKAMEEGCSPKEICDKYHAIHKDVYQWFNISFDEFGRTSSPQQ 120

Query: 121 TEVCQAIFGKLLKNNWLSENTMQQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQCEK 180
           TEVCQAIF KLL+NNWLSENTMQQLYCDTC+RFLADRLVEG CPTPGC YDSARGDQCEK
Sbjct: 121 TEVCQAIFKKLLENNWLSENTMQQLYCDTCKRFLADRLVEGNCPTPGCNYDSARGDQCEK 180

Query: 181 CGKLLNPTELKDPRCK 197
           CGKLLNPTEL DP+CK
Sbjct: 181 CGKLLNPTELLDPKCK 192

[Close]

BLAST of Cla010567 vs. NCBI nr
Match: gi|976924255|gb|KVI08255.1| (Aminoacyl-tRNA synthetase, class 1a, anticodon-binding [Cynara cardunculus var. scolymus])

HSP 1 Score: 371.7 bits (953), Expect = 8.6e-100
Identity = 171/197 (86.80%), Postives = 181/197 (91.88%), Query Frame = 1


Query: 1   MGDVS-GAAAGTKTTKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRLR 60
           MGD+  G     K TKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRLR
Sbjct: 1   MGDIPIGGGEDRKATKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRLR 60

Query: 61  GYNAIYICGTDEYGTATETKAMEEKCSPKEICDKYHAIHKEVYNWFGISFDEFGRTSSPQ 120
           GYNAIY+CGTDEYGTATETKA+EE C+PKEICDKYHAIH++VYNWF ISFDEFGRTS+PQ
Sbjct: 61  GYNAIYVCGTDEYGTATETKALEENCTPKEICDKYHAIHRDVYNWFNISFDEFGRTSTPQ 120

Query: 121 QTEVCQAIFGKLLKNNWLSENTMQQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQCE 180
           QTEVCQAIF KL++NNWLSENTMQQLYCDTC +FLADRLVEG CPTPGC YDSARGDQCE
Sbjct: 121 QTEVCQAIFKKLMENNWLSENTMQQLYCDTCNKFLADRLVEGSCPTPGCNYDSARGDQCE 180

Query: 181 KCGKLLNPTELKDPRCK 197
           KCGKLLNPTEL  PRCK
Sbjct: 181 KCGKLLNPTELISPRCK 197

[Close]

BLAST of Cla010567 vs. NCBI nr
Match: gi|848915972|ref|XP_012855641.1| (PREDICTED: probable methionine--tRNA ligase [Erythranthe guttata])

HSP 1 Score: 371.3 bits (952), Expect = 1.1e-99
Identity = 174/198 (87.88%), Postives = 181/198 (91.41%), Query Frame = 1


Query: 1   MGDVSGAAAGTKTT--KLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRL 60
           MGD S AAA    T  KLPIPGKRNILITSALPYVNN+PHLGNIIGCVLSADVFARYCRL
Sbjct: 1   MGDCSAAAAAAAATSAKLPIPGKRNILITSALPYVNNIPHLGNIIGCVLSADVFARYCRL 60

Query: 61  RGYNAIYICGTDEYGTATETKAMEEKCSPKEICDKYHAIHKEVYNWFGISFDEFGRTSSP 120
           RGYNAIYICGTDEYGTATETKA+EE CSPKEICDKYHAIHKEVY WF ISFDEFGRTSSP
Sbjct: 61  RGYNAIYICGTDEYGTATETKALEEGCSPKEICDKYHAIHKEVYKWFNISFDEFGRTSSP 120

Query: 121 QQTEVCQAIFGKLLKNNWLSENTMQQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQC 180
           QQTEVCQ IF KLL NNWLSENTMQQLYCDTC++FLADRLV+G CPTPGC YDSARGDQC
Sbjct: 121 QQTEVCQDIFKKLLDNNWLSENTMQQLYCDTCDKFLADRLVDGNCPTPGCNYDSARGDQC 180

Query: 181 EKCGKLLNPTELKDPRCK 197
           EKCGKLLNPTEL +P+CK
Sbjct: 181 EKCGKLLNPTELLEPKCK 198

The following BLAST results are available for this feature:

BLAST of Cla010567 vs. Swiss-Prot


Match Name	E-value	Identity	Description
SYMC_ARATH	1.6e-98	87.70	Methionine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana GN=At4g13780 PE=2 S...	[more]
SYM_ORYSJ	4.4e-96	86.81	Probable methionine--tRNA ligase OS=Oryza sativa subsp. japonica GN=Os06g0508700...	[more]
SYMC_MOUSE	8.7e-76	70.17	Methionine--tRNA ligase, cytoplasmic OS=Mus musculus GN=Mars PE=1 SV=1	[more]
SYMC_DICDI	2.8e-74	69.52	Probable methionine--tRNA ligase, cytoplasmic OS=Dictyostelium discoideum GN=met...	[more]
SYMC_CAEEL	1.1e-73	64.18	Methionine--tRNA ligase, cytoplasmic OS=Caenorhabditis elegans GN=mrs-1 PE=3 SV=...	[more]

BLAST of Cla010567 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LN88_CUCSA	1.1e-109	95.92	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G120970 PE=3 SV=1	[more]
A0A103YFP8_CYNCS	6.0e-100	86.80	Aminoacyl-tRNA synthetase, class 1a, anticodon-binding OS=Cynara cardunculus var...	[more]
A0A022Q3M2_ERYGU	7.9e-100	87.88	Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a001483mg PE=3 SV=1	[more]
K7LSI4_SOYBN	1.8e-99	91.89	Uncharacterized protein OS=Glycine max PE=3 SV=1	[more]
K7LSI3_SOYBN	1.8e-99	91.89	Uncharacterized protein OS=Glycine max PE=3 SV=1	[more]

BLAST of Cla010567 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659114634\|ref\|XP_008457156.1\|	8.3e-111	96.94	PREDICTED: probable methionine--tRNA ligase [Cucumis melo]	[more]
gi\|449442335\|ref\|XP_004138937.1\|	1.6e-109	95.92	PREDICTED: probable methionine--tRNA ligase [Cucumis sativus]	[more]
gi\|747106523\|ref\|XP_011101554.1\|	1.3e-100	89.80	PREDICTED: probable methionine--tRNA ligase [Sesamum indicum]	[more]
gi\|976924255\|gb\|KVI08255.1\|	8.6e-100	86.80	Aminoacyl-tRNA synthetase, class 1a, anticodon-binding [Cynara cardunculus var. ...	[more]
gi\|848915972\|ref\|XP_012855641.1\|	1.1e-99	87.88	PREDICTED: probable methionine--tRNA ligase [Erythranthe guttata]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR001412	aa-tRNA-synth_I_CS
IPR014729	Rossmann-like_a/b/a_fold
IPR014758	Met-tRNA_synth
IPR015413	Methionyl/Leucyl_tRNA_Synth

Vocabulary: **Molecular Function**
Term	Definition
GO:0000166	nucleotide binding
GO:0004812	aminoacyl-tRNA ligase activity
GO:0005524	ATP binding
GO:0004825	methionine-tRNA ligase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006418	tRNA aminoacylation for protein translation
GO:0006431	methionyl-tRNA aminoacylation

Vocabulary: **Cellular Component**
Term	Definition
GO:0005737	cytoplasm

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006431	methionyl-tRNA aminoacylation
biological_process	GO:0006418	tRNA aminoacylation for protein translation
biological_process	GO:0006555	methionine metabolic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004812	aminoacyl-tRNA ligase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004825	methionine-tRNA ligase activity
molecular_function	GO:0000166	nucleotide binding
molecular_function	GO:0000049	tRNA binding

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cla010567	Cla010567	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cla010567	Cla010567.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cla010567.1.cds1	Cla010567.1.cds1	CDS
Cla010567.1.cds2	Cla010567.1.cds2	CDS
Cla010567.1.cds3	Cla010567.1.cds3	CDS
Cla010567.1.cds4	Cla010567.1.cds4	CDS
Cla010567.1.cds5	Cla010567.1.cds5	CDS

InterPro

Analysis Name: InterPro Annotations of watermelon (97103)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001412	Aminoacyl-tRNA synthetase, class I, conserved site	PROSITE	PS00178	AA_TRNA_LIGASE_I	coord: 31..42 scor
IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	GENE3D	G3DSA:3.40.50.620		coord: 22..161 score: 5.6
IPR014758	Methionyl-tRNA synthetase	PRINTS	PR01041	TRNASYNTHMET	coord: 26..39 score: 2.5E-15coord: 107..118 score: 2.5E-15coord: 59..73 score: 2.5
IPR015413	Methionyl/Leucyl tRNA synthetase	PFAM	PF09334	tRNA-synt_1g	coord: 24..198 score: 1.4
None	No IPR available	PANTHER	PTHR11946	ISOLEUCYL, LEUCYL, TYROSYL, VALYL AND METHIONYL-TRNA SYNTHETASES	coord: 16..196 score: 1.0E
None	No IPR available	PANTHER	PTHR11946:SF78	SUBFAMILY NOT NAMED	coord: 16..196 score: 1.0E
None	No IPR available	unknown	SSF52374	Nucleotidylyl transferase	coord: 21..190 score: 2.25