Carg16486-RA (mRNA) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg16486-RA
Type	mRNA
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	light-inducible protein CPRF2-like
Location	Cucurbita_argyrosperma_scaffold_130 : 929456 .. 932340 (+)
Sequence length	1212

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AGGTTTGAAGAGTTTCAAAACTCTGTAATCGCGAAGCAGAAAAACCTGAGGCGGAAATTCATGAGATCAATGGATAGAGTATTCTCCGTTGACGGAATCGCCGATCAGTTCTTGTCCTCGCCGCCGCGAGCAGTTCCTACAGAGCCGGAAGAATCGTCGAAATTGAACCGGAGCGCATCGGAATGGTCGTTCCGGCGCTTTCTTCAGGAAGCCTCTGTATCGGACTCCTCGACTACTCCGCCTCCGCCGCCGGCTTCTCCCTCCCCCGACGATCAATCTGAGAATAAGGAATCTGGAGAAAATGCGAATCAGATTCTGCAGAAACAGAGTAATCGGAATGAAATATGTAGTAGTAGTAGTGCGACGTCGAGGACGGAGAAGAGTAAGGCGGCGGAATCCGTCAAGATTGGACCGCCGTGTGTTCCACCGGACTCCGGTGACTATCAGGCGTTTCTGAAAAGCAAGCTCAATTTGGCGTGTGCCGCTGTAGCCTTGTGCCGAGTAAGATTTTATATTTACTTTTTACTCTTTGGTTAAAAATCATGATTAGTTTTTCCATTTTTACCATTTTTACCTTTTCATTATTTGTAATTTTTATTTCAATAATATTTTAAAATATATTTTCATGTGCTTAATCCATTTAAAATATATGTTTTTCTAGATAATTTTCTAATTTTTTATAATCTTGTTCGAGTTATTCAATATTATAATTTTTTTCCTACCCTCGAGTCTTCACCCGATTCATTAATACCTTCAAATTTTTTAAAAATTTTCAAAAACGCTTTTAATTTTTTTAAAAAATAAAAAATAAAACGTTTAAAAATTTAAAAATTTAAAATTTAGTGGTTCCTCGATTCATGTGAAATAGGGGTCTTATATGATGAAAGCTCAAGATTCATGTGCAAGTTCTCCTCTAGCCTCACATTTGTCGTCTCAACCTTTAAAAGGTTTGCAATTTTGTTAAGAATGAGAAATAAAATTGAGATCGAGAGTGCAGCAAACATTGATGGGACTTTAGATCCTACTGGGACTTTTGATCCCACCAATGTTTGTTGCACCTTCGATTGCAATTCTAATTCTCATTTTTAATAAATTTTTATTTTTGCCCACATTTTTAATCTATTTTTTTTCCTTACAAGAATATTATTTTTGATGATCATGTTATAAAGATTAATGAACCTTTAGGAATTACTAGCTCACCTTGTGTGCAAAAAAAGGTCGGGATTCTTGGTAGCACGGCAACTATATCGTCATCCATAGAGCAAACGACTGATGAAGAAGATGATGTCGTCGAAGGAGAAGATGGTACGAGTGAACGTAAGGATCCTGCTTATGCTAAACGTGTCAGGAGGTAAATATTATAACATTTTGACTTTGTTATATTTTATAGTTCTAATTTAGTAGAGTAATAAATTTGGTCGTTAATGGTTGTGTCTGTTTAGCTTCTAGAGTACATCGTGCCAAATTAGTCCACTTGTCTAGTAAACATGTCACGTCGTCATTCAGTTTGTTTGAGATGGATAATCACGTTGATCTGGATCCATCAAAGGATAATACACAAAAAGTGTAATAGTCCAACCGTAACGCTAGCAGATATTATTTTCTTTGGACTTTCCATTCAATGGCTTCTCCTCAAGGTTTTAAAACGCGTCTTCTAGGGAAATGTTTTCATACCCTTAATGTTTTGTTTTCCTCTCCAACCGATGTGGGACTTCACAATCCAACCCTTTTGGAGGCTAGTGTCCTCGTTGGCACACCGTTCGATGTCTATAATACCATTTGTAACAATTCAAAGAGGACAATATCTACTAAAGGTGGGCTTGAGATGTTACCTTACAACCATTTAAAATGTATGCGCTAGGTAATATGAATATTTGGTTGCACTCATTTTGTTCGAGATGTCAATCTTCATCGTACCAAGTTAGATCCCAATTCCTTAAAATGCGTACTTCCACGACGACTCTCATGTCCAAAAGGAATACTGAAGTTGTCGTAGCCTTACCAAGTATTTGTCTCTCTCGATCCAAAGAGATACTAAAATTTCGGTTGGGAAGTTGAGTTATTTGATAGTGACTCGACCAAATATTACTTCTTCAACTAGCGTTGTAAGTCACTATATGTCTTCACGATTACCAACATGAAGAACCCAATCAAACTCCAACAAAAACCGTTATCGTAGATACTAACACAGTTATTTTCTTCAGAATGCTTTCTAACAGAGAATCAGCTAGACGTTCAAGAAAGAGAAAGCAAGCACATTTGACTGAGCTCGAGACACAGGTCAACATCGAACAATATCAAATCTATCTAATTCTAATAAGTCCATTATAAACTCGATTGGATTCATTGTTGATAGGTTGCTGAATTAAGAGTTGAAAATTCTGCATTGCTGAAGCGTTTCAATGATATAAGCCAGAAGTACAATGAATCAGCTGTTAATAACAGAGTTCTAAAAGCTGACCTTGAAACTTTGAAAGCAAAGGTAAGTACAAAGTACTAGCTCGTTCTTCATTCGTTCCGTGTTGATACGATGATGACGAGAACGATGACGTTTTCAGGTACAAATGGCTGAGGAAACCGTAAAGCGAATTACGGGTTCGAAACCGATGTTCTATGGCATGTCGTCAGAAGTATTGTCAATGAGCATGCAATCCTTTGATGGGAGCCCCTCAAAGACATCAAGAGATGCAGCTAGCAATCATGCTGCCGACATATCTTCTGCTGATACTCAGCTGAATTGTGTGGCAAAAGCAGCAACCGTGTCAGGGAAGACGATGGTGAAAACAGGTTCCTTGCGTCGAGTGGCAAGTTTGGAGCGCCTTCAAAAGCGCATCCGGGGGAGCTCAGGGTGCTGTGAACCATCGGGAAAGGGAGATCAGCAATAA

mRNA sequence

AGGTTTGAAGAGTTTCAAAACTCTGTAATCGCGAAGCAGAAAAACCTGAGGCGGAAATTCATGAGATCAATGGATAGAGTATTCTCCGTTGACGGAATCGCCGATCAGTTCTTGTCCTCGCCGCCGCGAGCAGTTCCTACAGAGCCGGAAGAATCGTCGAAATTGAACCGGAGCGCATCGGAATGGTCGTTCCGGCGCTTTCTTCAGGAAGCCTCTGTATCGGACTCCTCGACTACTCCGCCTCCGCCGCCGGCTTCTCCCTCCCCCGACGATCAATCTGAGAATAAGGAATCTGGAGAAAATGCGAATCAGATTCTGCAGAAACAGAGTAATCGGAATGAAATATGTAGTAGTAGTAGTGCGACGTCGAGGACGGAGAAGAGTAAGGCGGCGGAATCCGTCAAGATTGGACCGCCGTGTGTTCCACCGGACTCCGGTGACTATCAGGCGTTTCTGAAAAGCAAGCTCAATTTGGCGTGTGCCGCTGTAGCCTTGTGCCGAATTAATGAACCTTTAGGAATTACTAGCTCACCTTGTGTGCAAAAAAAGGTCGGGATTCTTGGTAGCACGGCAACTATATCGTCATCCATAGAGCAAACGACTGATGAAGAAGATGATGTCGTCGAAGGAGAAGATGGTACGAGTGAACGTAAGGATCCTGCTTATGCTAAACGTGTCAGGAGAATGCTTTCTAACAGAGAATCAGCTAGACGTTCAAGAAAGAGAAAGCAAGCACATTTGACTGAGCTCGAGACACAGGTTGCTGAATTAAGAGTTGAAAATTCTGCATTGCTGAAGCGTTTCAATGATATAAGCCAGAAGTACAATGAATCAGCTGTTAATAACAGAGTTCTAAAAGCTGACCTTGAAACTTTGAAAGCAAAGGTACAAATGGCTGAGGAAACCGTAAAGCGAATTACGGGTTCGAAACCGATGTTCTATGGCATGTCGTCAGAAGTATTGTCAATGAGCATGCAATCCTTTGATGGGAGCCCCTCAAAGACATCAAGAGATGCAGCTAGCAATCATGCTGCCGACATATCTTCTGCTGATACTCAGCTGAATTGTGTGGCAAAAGCAGCAACCGTGTCAGGGAAGACGATGGTGAAAACAGGTTCCTTGCGTCGAGTGGCAAGTTTGGAGCGCCTTCAAAAGCGCATCCGGGGGAGCTCAGGGTGCTGTGAACCATCGGGAAAGGGAGATCAGCAATAA

Coding sequence (CDS)

ATGAGATCAATGGATAGAGTATTCTCCGTTGACGGAATCGCCGATCAGTTCTTGTCCTCGCCGCCGCGAGCAGTTCCTACAGAGCCGGAAGAATCGTCGAAATTGAACCGGAGCGCATCGGAATGGTCGTTCCGGCGCTTTCTTCAGGAAGCCTCTGTATCGGACTCCTCGACTACTCCGCCTCCGCCGCCGGCTTCTCCCTCCCCCGACGATCAATCTGAGAATAAGGAATCTGGAGAAAATGCGAATCAGATTCTGCAGAAACAGAGTAATCGGAATGAAATATGTAGTAGTAGTAGTGCGACGTCGAGGACGGAGAAGAGTAAGGCGGCGGAATCCGTCAAGATTGGACCGCCGTGTGTTCCACCGGACTCCGGTGACTATCAGGCGTTTCTGAAAAGCAAGCTCAATTTGGCGTGTGCCGCTGTAGCCTTGTGCCGAATTAATGAACCTTTAGGAATTACTAGCTCACCTTGTGTGCAAAAAAAGGTCGGGATTCTTGGTAGCACGGCAACTATATCGTCATCCATAGAGCAAACGACTGATGAAGAAGATGATGTCGTCGAAGGAGAAGATGGTACGAGTGAACGTAAGGATCCTGCTTATGCTAAACGTGTCAGGAGAATGCTTTCTAACAGAGAATCAGCTAGACGTTCAAGAAAGAGAAAGCAAGCACATTTGACTGAGCTCGAGACACAGGTTGCTGAATTAAGAGTTGAAAATTCTGCATTGCTGAAGCGTTTCAATGATATAAGCCAGAAGTACAATGAATCAGCTGTTAATAACAGAGTTCTAAAAGCTGACCTTGAAACTTTGAAAGCAAAGGTACAAATGGCTGAGGAAACCGTAAAGCGAATTACGGGTTCGAAACCGATGTTCTATGGCATGTCGTCAGAAGTATTGTCAATGAGCATGCAATCCTTTGATGGGAGCCCCTCAAAGACATCAAGAGATGCAGCTAGCAATCATGCTGCCGACATATCTTCTGCTGATACTCAGCTGAATTGTGTGGCAAAAGCAGCAACCGTGTCAGGGAAGACGATGGTGAAAACAGGTTCCTTGCGTCGAGTGGCAAGTTTGGAGCGCCTTCAAAAGCGCATCCGGGGGAGCTCAGGGTGCTGTGAACCATCGGGAAAGGGAGATCAGCAATAA

Protein sequence

MRSMDRVFSVDGIADQFLSSPPRAVPTEPEESSKLNRSASEWSFRRFLQEASVSDSSTTPPPPPASPSPDDQSENKESGENANQILQKQSNRNEICSSSSATSRTEKSKAAESVKIGPPCVPPDSGDYQAFLKSKLNLACAAVALCRINEPLGITSSPCVQKKVGILGSTATISSSIEQTTDEEDDVVEGEDGTSERKDPAYAKRVRRMLSNRESARRSRKRKQAHLTELETQVAELRVENSALLKRFNDISQKYNESAVNNRVLKADLETLKAKVQMAEETVKRITGSKPMFYGMSSEVLSMSMQSFDGSPSKTSRDAASNHAADISSADTQLNCVAKAATVSGKTMVKTGSLRRVASLERLQKRIRGSSGCCEPSGKGDQQ

Homology

[Close]

BLAST of Carg16486-RA vs. NCBI nr
Match: XP_023529858.1 (light-inducible protein CPRF2-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 606.7 bits (1563), Expect = 5.5e-170
Identity = 368/400 (92.00%), Postives = 370/400 (92.50%), Query Frame = 0


Query: 1   MRSMDRVFSVDGIADQFLSSPPRAVPTEPEESSKLNRSASEWSFRRFLQEASVSXXXXXX 60
           MRSMDRVFSVDGIADQFLSSPPRAVPTEPEESSKLNRSASEWSFRRFLQEASV XXXXXX
Sbjct: 1   MRSMDRVFSVDGIADQFLSSPPRAVPTEPEESSKLNRSASEWSFRRFLQEASVXXXXXXX 60

Query: 61  XXXXXXXXXXXXXXXXXXXXNANQILQKQSNRNEICSSSSATSRTEKSKAAESVKIGPPC 120
           XXXXXXXXXXXXXXXXXX  NANQILQKQSNRNEICSSSSATSR EK KAAESVKIGPPC
Sbjct: 61  XXXXXXXXXXXXXXXXXXGENANQILQKQSNRNEICSSSSATSRMEKIKAAESVKIGPPC 120

Query: 121 VPPDSGDYQAFLKSKLNLACAAVALCR-----------INEPL------GITSSPCVQKK 180
           VP DSGDYQA LKSKLNLACAAVALCR            + PL      GITSSPCVQKK
Sbjct: 121 VPSDSGDYQALLKSKLNLACAAVALCRGSYMMKAQDSCASSPLASQSSKGITSSPCVQKK 180

Query: 181 VGILGSTATISSSIEQTTDEEDDVVEGEDGTSERKDPAYAKRVRRMLSNRESARRSRKRK 240
           VGILGS+ATISSSIEQTTDEEDDVVEGEDGTSERKDPAYAKRVRRMLSNRESARRSRKRK
Sbjct: 181 VGILGSSATISSSIEQTTDEEDDVVEGEDGTSERKDPAYAKRVRRMLSNRESARRSRKRK 240

Query: 241 QAHLTELETQVAELRVENSALLKRFNDISQKYNESAVNNRVLKADLETLKAKVQMAEETV 300
           QAHLTELETQVAELRVENSALLK FNDISQKYNESAVNNRVLKADLETLKAKVQMAEETV
Sbjct: 241 QAHLTELETQVAELRVENSALLKHFNDISQKYNESAVNNRVLKADLETLKAKVQMAEETV 300

Query: 301 KRITGSKPMFYGMSSEVLSMSMQSFDGSPSKTSRDAASNHAADISSADTQLNCVAKAATV 360
           KRITGSKPMFYGMSSE LSMSMQSFDGSPSKTSRDAASNHAADISSADTQLNCVAKAATV
Sbjct: 301 KRITGSKPMFYGMSSEGLSMSMQSFDGSPSKTSRDAASNHAADISSADTQLNCVAKAATV 360

Query: 361 SGKTMVKTGSLRRVASLERLQKRIRGSSGCCEPSGKGDQQ 384
           SGK MVKTGSLRRVASLERLQKRIRGSSGCCEPSGKGD Q
Sbjct: 361 SGKKMVKTGSLRRVASLERLQKRIRGSSGCCEPSGKGDHQ 400

[Close]

BLAST of Carg16486-RA vs. NCBI nr
Match: XP_022926013.1 (light-inducible protein CPRF2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 598.2 bits (1541), Expect = 2.0e-167
Identity = 366/403 (90.82%), Postives = 369/403 (91.56%), Query Frame = 0


Query: 1   MRSMDRVFSVDGIADQFLSSPPRAVPTEPEESSKLNRSASEWSFRRFLQEASVSXXXXXX 60
           MRSMDRVFSVDGIADQFLSSPPRAVPTEPEESSKLNRSASEWSFRRFLQEASV XXXXXX
Sbjct: 1   MRSMDRVFSVDGIADQFLSSPPRAVPTEPEESSKLNRSASEWSFRRFLQEASVXXXXXXX 60

Query: 61  XXXXXXXXXXXXXXXXXXXXNANQILQKQSNRNEICSSSSATSRTEKSKAAESVKIGPPC 120
           XXXXXXXXXXXXXXXXXXXX    ILQKQSNRNEICSS     RTEKSKAAESV+IGPPC
Sbjct: 61  XXXXXXXXXXXXXXXXXXXXXXXXILQKQSNRNEICSSXXXXXRTEKSKAAESVEIGPPC 120

Query: 121 VPPDSGDYQAFLKSKLNLACAAVALCR--------------------INEPL-GITSSPC 180
           VP DSGDYQAFLKSKLNLACAAVALCR                     ++PL GITSSPC
Sbjct: 121 VPSDSGDYQAFLKSKLNLACAAVALCRGSYMMKVQDSCASSLLASYLQSQPLKGITSSPC 180

Query: 181 VQKKVGILGSTATISSSIEQTTDEEDDVVEGEDGTSERKDPAYAKRVRRMLSNRESARRS 240
           VQKKVGILG TATISSSIEQTTDEEDDVVEGEDGTSERKDPAYAKRVRRMLSNRESARRS
Sbjct: 181 VQKKVGILGGTATISSSIEQTTDEEDDVVEGEDGTSERKDPAYAKRVRRMLSNRESARRS 240

Query: 241 RKRKQAHLTELETQVAELRVENSALLKRFNDISQKYNESAVNNRVLKADLETLKAKVQMA 300
           RKRKQAHLTELETQVAELRVENSALLKRFNDISQKYNESAVNNRVLKADLETLKAKVQMA
Sbjct: 241 RKRKQAHLTELETQVAELRVENSALLKRFNDISQKYNESAVNNRVLKADLETLKAKVQMA 300

Query: 301 EETVKRITGSKPMFYGMSSEVLSMSMQSFDGSPSKTSRDAASNHAADISSADTQLNCVAK 360
           EETVKRITGSKPMFYGMSSEVLSMSMQSFDGSPSKTSRDAASNHAADISSADTQLNCVAK
Sbjct: 301 EETVKRITGSKPMFYGMSSEVLSMSMQSFDGSPSKTSRDAASNHAADISSADTQLNCVAK 360

Query: 361 AATVSGKTMVKTGSLRRVASLERLQKRIRGSSGCCEPSGKGDQ 383
           AATVSGKTMVKTGSLRRVASLERLQKRIRGSSGCCEPSGKGDQ
Sbjct: 361 AATVSGKTMVKTGSLRRVASLERLQKRIRGSSGCCEPSGKGDQ 403

[Close]

BLAST of Carg16486-RA vs. NCBI nr
Match: XP_023002073.1 (light-inducible protein CPRF2-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 588.2 bits (1515), Expect = 2.0e-164
Identity = 360/404 (89.11%), Postives = 368/404 (91.09%), Query Frame = 0


Query: 1   MRSMDRVFSVDGIADQFLSSPPRAVPTEPEESSKLNRSASEWSFRRFLQEASVSXXXXXX 60
           MRSMDRVFSVDGIADQFLSSPPRAVPTEPEESSKLNRSASEWSFRRFLQEASV XXXXXX
Sbjct: 1   MRSMDRVFSVDGIADQFLSSPPRAVPTEPEESSKLNRSASEWSFRRFLQEASVXXXXXXX 60

Query: 61  XXXXXXXXXXXXXXXXXXXXNANQILQKQSNRNEICSSSSATSRTEKSKAAESVKIGPPC 120
           XXXXXXXXXXXXXXXXXXX NANQILQKQSNRNEICSSSSATSR EKSKAAESVKIGPPC
Sbjct: 61  XXXXXXXXXXXXXXXXXXXENANQILQKQSNRNEICSSSSATSRMEKSKAAESVKIGPPC 120

Query: 121 VPPDSGDYQAFLKSKLNLACAAVALCR-----------INEPL----------GITSSPC 180
           VPP+SGDYQAFLKSKLNLACAAVALCR            + PL          GITSSPC
Sbjct: 121 VPPNSGDYQAFLKSKLNLACAAVALCRGSYMMKAQDSCASSPLASHLSFQHSKGITSSPC 180

Query: 181 VQKKVGILGSTATISSSIEQTTDEEDDVVEGEDGTSERKDPAYAKRVRRMLSNRESARRS 240
           VQKKVGILGS+ATISSSI+QTTDEEDDVVEGEDGTSE KDPA+AKRVRRMLSNRESARRS
Sbjct: 181 VQKKVGILGSSATISSSIDQTTDEEDDVVEGEDGTSEHKDPAHAKRVRRMLSNRESARRS 240

Query: 241 RKRKQAHLTELETQVAELRVENSALLKRFNDISQKYNESAVNNRVLKADLETLKAKVQMA 300
           RKRKQAHLTELETQVAELRVENSALLKRFNDISQKYNESA+NNRVLKADLETLKAKVQMA
Sbjct: 241 RKRKQAHLTELETQVAELRVENSALLKRFNDISQKYNESAINNRVLKADLETLKAKVQMA 300

Query: 301 EETVKRITGSKPMFYGMSSEVLSMSMQSFDGSPSKTSRDAASNHAADISSADTQLNCVAK 360
           EETV+RI GSKPMFYG   EV SMSMQSFDGSPSKTSRDAASNHAADISSADTQLNCVAK
Sbjct: 301 EETVRRIMGSKPMFYG---EVSSMSMQSFDGSPSKTSRDAASNHAADISSADTQLNCVAK 360

Query: 361 AATVSGKTMVKTGSLRRVASLERLQKRIRGSSGCCEPSGKGDQQ 384
           AATVSGK MVKTGSL RVASLE LQKRIRGSSGCC+PSGKG QQ
Sbjct: 361 AATVSGKKMVKTGSLHRVASLESLQKRIRGSSGCCKPSGKGGQQ 401

[Close]

BLAST of Carg16486-RA vs. NCBI nr
Match: XP_023529859.1 (light-inducible protein CPRF2-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 584.7 bits (1506), Expect = 2.3e-163
Identity = 355/383 (92.69%), Postives = 356/383 (92.95%), Query Frame = 0


Query: 1   MRSMDRVFSVDGIADQFLSSPPRAVPTEPEESSKLNRSASEWSFRRFLQEASVSXXXXXX 60
           MRSMDRVFSVDGIADQFLSSPPRAVPTEPEESSKLNRSASEWSFRRFLQEASV XXXXXX
Sbjct: 1   MRSMDRVFSVDGIADQFLSSPPRAVPTEPEESSKLNRSASEWSFRRFLQEASVXXXXXXX 60

Query: 61  XXXXXXXXXXXXXXXXXXXXNANQILQKQSNRNEICSSSSATSRTEKSKAAESVKIGPPC 120
           XXXXXXXXXXXXXXXXXX  NANQILQKQSNRNEICSSSSATSR EK KAAESVKIGPPC
Sbjct: 61  XXXXXXXXXXXXXXXXXXGENANQILQKQSNRNEICSSSSATSRMEKIKAAESVKIGPPC 120

Query: 121 VPPDSGDYQAFLKSKLNLACAAVALCRINEPLGITSSPCVQKKVGILGSTATISSSIEQT 180
           VP DSGDYQA LKSKLNLACAAVALCR                VGILGS+ATISSSIEQT
Sbjct: 121 VPSDSGDYQALLKSKLNLACAAVALCR----------------VGILGSSATISSSIEQT 180

Query: 181 TDEEDDVVEGEDGTSERKDPAYAKRVRRMLSNRESARRSRKRKQAHLTELETQVAELRVE 240
           TDEEDDVVEGEDGTSERKDPAYAKRVRRMLSNRESARRSRKRKQAHLTELETQVAELRVE
Sbjct: 181 TDEEDDVVEGEDGTSERKDPAYAKRVRRMLSNRESARRSRKRKQAHLTELETQVAELRVE 240

Query: 241 NSALLKRFNDISQKYNESAVNNRVLKADLETLKAKVQMAEETVKRITGSKPMFYGMSSEV 300
           NSALLK FNDISQKYNESAVNNRVLKADLETLKAKVQMAEETVKRITGSKPMFYGMSSE 
Sbjct: 241 NSALLKHFNDISQKYNESAVNNRVLKADLETLKAKVQMAEETVKRITGSKPMFYGMSSEG 300

Query: 301 LSMSMQSFDGSPSKTSRDAASNHAADISSADTQLNCVAKAATVSGKTMVKTGSLRRVASL 360
           LSMSMQSFDGSPSKTSRDAASNHAADISSADTQLNCVAKAATVSGK MVKTGSLRRVASL
Sbjct: 301 LSMSMQSFDGSPSKTSRDAASNHAADISSADTQLNCVAKAATVSGKKMVKTGSLRRVASL 360

Query: 361 ERLQKRIRGSSGCCEPSGKGDQQ 384
           ERLQKRIRGSSGCCEPSGKGD Q
Sbjct: 361 ERLQKRIRGSSGCCEPSGKGDHQ 367

[Close]

BLAST of Carg16486-RA vs. NCBI nr
Match: XP_022926023.1 (light-inducible protein CPRF2-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 577.8 bits (1488), Expect = 2.8e-161
Identity = 353/382 (92.41%), Postives = 354/382 (92.67%), Query Frame = 0


Query: 1   MRSMDRVFSVDGIADQFLSSPPRAVPTEPEESSKLNRSASEWSFRRFLQEASVSXXXXXX 60
           MRSMDRVFSVDGIADQFLSSPPRAVPTEPEESSKLNRSASEWSFRRFLQEASV XXXXXX
Sbjct: 1   MRSMDRVFSVDGIADQFLSSPPRAVPTEPEESSKLNRSASEWSFRRFLQEASVXXXXXXX 60

Query: 61  XXXXXXXXXXXXXXXXXXXXNANQILQKQSNRNEICSSSSATSRTEKSKAAESVKIGPPC 120
           XXXXXXXXXXXXXXXXXXXX    ILQKQSNRNEICSS     RTEKSKAAESV+IGPPC
Sbjct: 61  XXXXXXXXXXXXXXXXXXXXXXXXILQKQSNRNEICSSXXXXXRTEKSKAAESVEIGPPC 120

Query: 121 VPPDSGDYQAFLKSKLNLACAAVALCRINEPLGITSSPCVQKKVGILGSTATISSSIEQT 180
           VP DSGDYQAFLKSKLNLACAAVALCR                VGILG TATISSSIEQT
Sbjct: 121 VPSDSGDYQAFLKSKLNLACAAVALCR----------------VGILGGTATISSSIEQT 180

Query: 181 TDEEDDVVEGEDGTSERKDPAYAKRVRRMLSNRESARRSRKRKQAHLTELETQVAELRVE 240
           TDEEDDVVEGEDGTSERKDPAYAKRVRRMLSNRESARRSRKRKQAHLTELETQVAELRVE
Sbjct: 181 TDEEDDVVEGEDGTSERKDPAYAKRVRRMLSNRESARRSRKRKQAHLTELETQVAELRVE 240

Query: 241 NSALLKRFNDISQKYNESAVNNRVLKADLETLKAKVQMAEETVKRITGSKPMFYGMSSEV 300
           NSALLKRFNDISQKYNESAVNNRVLKADLETLKAKVQMAEETVKRITGSKPMFYGMSSEV
Sbjct: 241 NSALLKRFNDISQKYNESAVNNRVLKADLETLKAKVQMAEETVKRITGSKPMFYGMSSEV 300

Query: 301 LSMSMQSFDGSPSKTSRDAASNHAADISSADTQLNCVAKAATVSGKTMVKTGSLRRVASL 360
           LSMSMQSFDGSPSKTSRDAASNHAADISSADTQLNCVAKAATVSGKTMVKTGSLRRVASL
Sbjct: 301 LSMSMQSFDGSPSKTSRDAASNHAADISSADTQLNCVAKAATVSGKTMVKTGSLRRVASL 360

Query: 361 ERLQKRIRGSSGCCEPSGKGDQ 383
           ERLQKRIRGSSGCCEPSGKGDQ
Sbjct: 361 ERLQKRIRGSSGCCEPSGKGDQ 366

[Close]

BLAST of Carg16486-RA vs. TAIR10
Match: AT5G28770.2 (bZIP transcription factor family protein)

HSP 1 Score: 181.8 bits (460), Expect = 8.0e-46
Identity = 142/344 (41.28%), Postives = 183/344 (53.20%), Query Frame = 0


Query: 33  SKLNRSASEWSFRRFLQEASVSXXXXXXXXXXXXXXXXXXXXXXXXXXNANQILQKQSNR 92
           + LNRSASEW+F RF+QE                                          
Sbjct: 22  TSLNRSASEWAFNRFIQE------------------------------------------ 81

Query: 93  NEICSSSSATSRTEKSKAAESVKIGPPCVPPDSGDYQAFLKSKLNLACAAVALCR--INE 152
                SS+A    E + A       PP VP DS +Y+AFLKSKLNLACAAVA+ R    +
Sbjct: 82  -----SSAAADDGESTTACGVSVSSPPNVPVDSEEYRAFLKSKLNLACAAVAMKRGTFIK 141

Query: 153 PLGIT-----SSPCVQKKVGILGSTATISSSIEQTTDEEDDVVEGEDGTSERKDPAYAKR 212
           P   +           ++  +  S AT   S   T+  E              +P   KR
Sbjct: 142 PQDTSGRSDNGGANESEQASLASSKATPMMSSAITSGSELSXXXXXXDGETNMNPTNVKR 201

Query: 213 VRRMLSNRESARRSRKRKQAHLTELETQVAELRVENSALLKRFNDISQKYNESAVNNRVL 272
           V+RMLSNRESARRSR+RKQAHL+ELETQV++LRVENS L+K   D++Q +N+++V NRVL
Sbjct: 202 VKRMLSNRESARRSRRRKQAHLSELETQVSQLRVENSKLMKGLTDVTQTFNDASVENRVL 261

Query: 273 KADLETLKAKVQMAEETVKRITGSKPMFYGMSSEVLSMSMQS-FDGSPSKTSRDAASNHA 332
           KA++ETL+AKV+MAEETVKR+TG  PMF+ M   V ++S+ S    SP  TS    +   
Sbjct: 262 KANIETLRAKVKMAEETVKRLTGFNPMFHNMPQIVSTVSLPSETSNSPDTTSSQVTTPEI 309

Query: 333 ADISSADTQLNCVAKAATVSGKTMVKTGSLRRVASLERLQKRIR 369
             ISS +       K   + G  M +T S+RRV SLE LQKRIR
Sbjct: 322 --ISSGN-------KGKALIGCKMNRTASMRRVESLEHLQKRIR 309

[Close]

BLAST of Carg16486-RA vs. TAIR10
Match: AT4G02640.2 (bZIP transcription factor family protein)

HSP 1 Score: 126.7 bits (317), Expect = 3.0e-29
Identity = 108/340 (31.76%), Postives = 157/340 (46.18%), Query Frame = 0


Query: 4   MDRVFSVDGIADQFLSSPPRAVPTEPEES-----SKLNRSASEWSFRRFLQEASVSXXXX 63
           M+ +FS+D  +D F  +PP  +P  P+ S      ++++S  EW+F  FL+E S S    
Sbjct: 1   MNSIFSIDDFSDPFWETPP--IPLNPDSSKPVTADEVSQSQPEWTFEMFLEEISSS---- 60

Query: 64  XXXXXXXXXXXXXXXXXXXXXXNANQILQKQSNRNEICSSSSATSRTEKSKAAESVKIGP 123
                                 N N  +   S+   + S S      + S+  +      
Sbjct: 61  --------------AVSSEPLGNNNNAIVGVSSAQSLPSVSGQNDFEDDSRFRDRDSGNL 120

Query: 124 PCVPP--------DSGDYQAFLKSKLNLACAAVALCRINE-------------------- 183
            C  P        DS DY+  LK+KL   CA V   R+                      
Sbjct: 121 DCAAPMTTKTVIVDSDDYRRVLKNKLETECATVVSLRVGSVKPEDSTSSPETQLQPVQSS 180

Query: 184 -----------PLGITSS-PCVQKKVGILGSTATISSSIEQTTDEEDDVVEGEDGTSERK 243
                       LG+TSS P   KK G+     T  SS E + DE+   ++ E+ T+   
Sbjct: 181 PLTQGSLMTPGELGVTSSLPAEVKKTGVSMKQVTSGSSREYSDDED---LDEENETTGSL 240

Query: 244 DPAYAKRVRRMLSNRESARRSRKRKQAHLTELETQVAELRVENSALLKRFNDISQKYNES 299
            P   K+ RRMLSN               ++LETQV +L+ E+S+LLK+ ++++ KY+E+
Sbjct: 241 KPEDVKKSRRMLSNXXXXXXXXXXXXXQTSDLETQVNDLKGEHSSLLKQLSNMNHKYDEA 300

[Close]

BLAST of Carg16486-RA vs. TAIR10
Match: AT3G54620.1 (basic leucine zipper 25)

HSP 1 Score: 115.5 bits (288), Expect = 7.0e-26
Identity = 106/324 (32.72%), Postives = 158/324 (48.77%), Query Frame = 0


Query: 35  LNRSASEWSFRRFLQEASVSXXXXXXXXXXXXXXXXXXXXXXXXXXNANQ---ILQKQSN 94
           + RS SEW+F R + E S S                          +  +    +QK  N
Sbjct: 40  MTRSQSEWAFHRLINELSGSDSSPTTNTIERSPPPVQSLSRLEETVDETEDVVEIQKPQN 99

Query: 95  RNEICSSSSATSRTEKSKAAESVKIGPPCVPPDSGDYQAFLKSKLNLACAAVALCRIN-- 154
              +       +R  ++ +++ V    P V  D   Y A LKSKL LACAAVA  R+   
Sbjct: 100 HRRLPVDDQGKNR-NRAPSSDPVDSSAPVV-VDPNQYHAILKSKLELACAAVAR-RVGTV 159

Query: 155 EPLGITSSPCVQKKV--GILGSTATISSSIE----QTTDEEDDVVEGEDGTSERKD---- 214
           +P   ++S   QK+    I+  T+  +SS+      +T ++ DV   +   S R D    
Sbjct: 160 KPEDSSASASNQKQAQGSIVAQTSPGASSVRFSPTTSTQKKPDVPARQTSISSRDDXXXX 219

Query: 215 ----------PAYAKRVRRMLSNRESARRSRKRKQAHLTELETQVAELRVENSALLKRFN 274
                     P   KR RRMLSNRESAR      Q  + E +TQV +LR E+S L+ R +
Sbjct: 220 XXXXXXXXXXPTDVKRARRMLSNRESARXXXXXXQEQMNEFDTQVGQLRAEHSTLINRLS 279

Query: 275 DISQKYNESAVNNRVLKADLETLKAKVQMAEETVKRITGSKPMFYGMSSEVLSMSMQSFD 333
           D++ KY+ +AV+NR+L+AD+ETL+ KV+MAEETVKR+TG  P+ +   +  +      F 
Sbjct: 280 DMNHKYDAAAVDNRILRADIETLRTKVKMAEETVKRVTGVNPLHWSRPNMGI-----PFS 339

[Close]

BLAST of Carg16486-RA vs. TAIR10
Match: AT5G24800.1 (basic leucine zipper 9)

HSP 1 Score: 93.2 bits (230), Expect = 3.7e-19
Identity = 60/119 (50.42%), Postives = 80/119 (67.23%), Query Frame = 0


Query: 168 GSTATISSSIEQTTDEEDDVVEGEDGTSE-RKDPAYAKRVRRMLSNRESARRSRKRKQAH 227
           G   T S S    +D+ED   E E G SE   DP   KR+RRM SNRESA+RSR+RKQ +
Sbjct: 87  GVRRTTSGSSHVNSDDED--AETEAGQSEMTNDPNDLKRIRRMNSNRESAKRSRRRKQEY 146

Query: 228 LTELETQVAELRVENSALLKRFNDISQKYNESAVNNRVLKADLETLKAKVQMAEETVKR 286
           L +LETQV  L+ +NS L K+  D +Q++  +  NNRVLK+D+ETL+ KV++AE+ V R
Sbjct: 147 LVDLETQVDSLKGDNSTLYKQLIDATQQFRSAGTNNRVLKSDVETLRVKVKLAEDLVAR 203

[Close]

BLAST of Carg16486-RA vs. TAIR10
Match: AT5G15830.1 (basic leucine-zipper 3)

HSP 1 Score: 50.8 bits (120), Expect = 2.1e-06
Identity = 44/120 (36.67%), Postives = 62/120 (51.67%), Query Frame = 0


Query: 204 KRVRRMLSNRESARRSRKRKQAHLTELETQVAELRVENSALLKRFNDISQKYNESAVNNR 263
           ++ RRM+SNRESARRSR RKQ HL EL +QVA LR EN  LL + N +S   +     N 
Sbjct: 74  RKQRRMVSNRESARRSRMRKQRHLDELLSQVAWLRSENHQLLDKLNQVSDNNDLVIQENS 133

Query: 264 VLKADLETLKAKVQMAEETVKRITGSKPMFYGMSSEVLSMSMQSFDGSPSKTSRDAASNH 323
            LK +      +++    ++K++ G     Y   S     SM   D   S  + D  ++H
Sbjct: 134 SLKEE----NLELRQVITSMKKLGGGIHDKYSSPS-----SMDELDQDFSSITDDPRTHH 184

[Close]

BLAST of Carg16486-RA vs. Swiss-Prot
Match: sp|Q99090|CPRF2_PETCR (Light-inducible protein CPRF2 OS=Petroselinum crispum OX=4043 GN=CPRF2 PE=2 SV=2)

HSP 1 Score: 236.1 bits (601), Expect = 6.4e-61
Identity = 189/452 (41.81%), Postives = 232/452 (51.33%), Query Frame = 0


Query: 4   MDRVFSVDGIADQFLSSPPRAVPTEPEESSKL--NRSASEWSFRRFLQEASVSXXXXXXX 63
           MDRVFSV+ I+DQF S P R      E+SSKL  NRS SEW+F+ FLQ+AS         
Sbjct: 1   MDRVFSVEDISDQFWSPPAR------EDSSKLVMNRSDSEWAFQSFLQQAS--------- 60

Query: 64  XXXXXXXXXXXXXXXXXXXNANQILQKQSNRNEICSSSSATSRTEKSKAAESVKIG---P 123
                                                SS    ++    A  VK     P
Sbjct: 61  ----------------------------------ALESSQPLPSDPVPVAGDVKNPVEIP 120

Query: 124 PCVPPDSGDYQAFLKSKLNLACAAVALCRIN----------------------------- 183
             VP DS DYQA+LKS+L+LACAAVAL R +                             
Sbjct: 121 ANVPVDSEDYQAYLKSRLDLACAAVALTRASSLKPQDSAALLDNGSQASNTSQLVSQVPP 180

Query: 184 -------------EPLGITSS---PCVQKKVGILGSTATISSSIEQTTDEEDDVVEGEDG 243
                        E L  T++   P +QKK  I   + T  SS +         +EGE  
Sbjct: 181 KGSGHDLSKEEDKEALAATATPLLPALQKKSAIQVKSTTSGSSRDH--XXXXXXLEGETE 240

Query: 244 TSERKDPAYAKRVRRMLSNRESARRSRKRKQAHLTELETQVAELRVENSALLKRFNDISQ 303
           T+   DP+ AKRVRRMLSNRESARRSR+RKQAH+TELETQV++LRVENS+LLKR  DISQ
Sbjct: 241 TTRNGDPSDAKRVRRMLSNRESARRSRRRKQAHMTELETQVSQLRVENSSLLKRLTDISQ 300

Query: 304 KYNESAVNNRVLKADLETLKAKVQMAEETVKRITGSKPMFYGMSSEVLSMSMQSFDGSPS 363
           +YN++AV+NRVLKAD+ET++AKV+MAEETVKR+TG  PMF  MSSE+ ++ MQSF GSPS
Sbjct: 301 RYNDAAVDNRVLKADIETMRAKVKMAEETVKRVTGLNPMFQSMSSEISTIGMQSFSGSPS 360

Query: 364 KTSRDAASN-------HAADISSADTQ----------------LNCVAKAATVSGKTMVK 383
            TS D   +         A  S    Q                L   + +  V G  M +
Sbjct: 361 DTSADTTQDGSKQHFYQPAPTSHMPAQDQKIQNGLLQVPPVDNLQQHSASGPVEGNKMER 401

[Close]

BLAST of Carg16486-RA vs. Swiss-Prot
Match: sp|B9DGI8|BZP63_ARATH (Basic leucine zipper 63 OS=Arabidopsis thaliana OX=3702 GN=BZIP63 PE=1 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.4e-44
Identity = 142/344 (41.28%), Postives = 183/344 (53.20%), Query Frame = 0


Query: 33  SKLNRSASEWSFRRFLQEASVSXXXXXXXXXXXXXXXXXXXXXXXXXXNANQILQKQSNR 92
           + LNRSASEW+F RF+QE                                          
Sbjct: 22  TSLNRSASEWAFNRFIQE------------------------------------------ 81

Query: 93  NEICSSSSATSRTEKSKAAESVKIGPPCVPPDSGDYQAFLKSKLNLACAAVALCR--INE 152
                SS+A    E + A       PP VP DS +Y+AFLKSKLNLACAAVA+ R    +
Sbjct: 82  -----SSAAADDGESTTACGVSVSSPPNVPVDSEEYRAFLKSKLNLACAAVAMKRGTFIK 141

Query: 153 PLGIT-----SSPCVQKKVGILGSTATISSSIEQTTDEEDDVVEGEDGTSERKDPAYAKR 212
           P   +           ++  +  S AT   S   T+  E              +P   KR
Sbjct: 142 PQDTSGRSDNGGANESEQASLASSKATPMMSSAITSGSELSXXXXXXDGETNMNPTNVKR 201

Query: 213 VRRMLSNRESARRSRKRKQAHLTELETQVAELRVENSALLKRFNDISQKYNESAVNNRVL 272
           V+RMLSNRESARRSR+RKQAHL+ELETQV++LRVENS L+K   D++Q +N+++V NRVL
Sbjct: 202 VKRMLSNRESARRSRRRKQAHLSELETQVSQLRVENSKLMKGLTDVTQTFNDASVENRVL 261

Query: 273 KADLETLKAKVQMAEETVKRITGSKPMFYGMSSEVLSMSMQS-FDGSPSKTSRDAASNHA 332
           KA++ETL+AKV+MAEETVKR+TG  PMF+ M   V ++S+ S    SP  TS    +   
Sbjct: 262 KANIETLRAKVKMAEETVKRLTGFNPMFHNMPQIVSTVSLPSETSNSPDTTSSQVTTPEI 309

Query: 333 ADISSADTQLNCVAKAATVSGKTMVKTGSLRRVASLERLQKRIR 369
             ISS +       K   + G  M +T S+RRV SLE LQKRIR
Sbjct: 322 --ISSGN-------KGKALIGCKMNRTASMRRVESLEHLQKRIR 309

[Close]

BLAST of Carg16486-RA vs. Swiss-Prot
Match: sp|Q7X9A8|RSBZ2_ORYSJ (bZIP transcription factor RISBZ2 OS=Oryza sativa subsp. japonica OX=39947 GN=RISBZ2 PE=1 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 6.3e-32
Identity = 98/201 (48.76%), Postives = 132/201 (65.67%), Query Frame = 0


Query: 189 EGEDGTSERKDPAYAKRVRRMLSNRESARRSRKRKQAHLTELETQVAELRVENSALLKRF 248
           EGE  T+    PA  +  RR  SNRESARRSR RK AHL ELE QV++LRVENS+LL+R 
Sbjct: 219 EGEAETTGTARPADQRLQRRKQSNRESARRSRSRKAAHLNELEAQVSQLRVENSSLLRRL 278

Query: 249 NDISQKYNESAVNNRVLKADLETLKAKVQMAEETVKRITGSKPMFYGMSSEVLSMSMQSF 308
            D++QKYN++AV+NRVLKAD+ETL+AKV+MAE++VKR+TG   +F   +S++ S+SM  F
Sbjct: 279 ADVNQKYNDAAVDNRVLKADVETLRAKVKMAEDSVKRVTGMNALF-PAASDMSSLSM-PF 338

Query: 309 DGSPSKTSRDAA--------------------SNHAADISSADTQLNCVAKAATVSGKTM 368
           + SPS+ + DAA                    +N+  DI S+  +       A  +GK +
Sbjct: 339 NSSPSEATSDAAVPIQDDPNNYFATNNDIGGNNNYMPDIPSSAQEDEDFVNGALAAGK-I 398

Query: 369 VKTGSLRRVASLERLQKRIRG 370
            +T SL+RVASLE LQKR+ G
Sbjct: 399 GRTASLQRVASLEHLQKRMCG 416

[Close]

BLAST of Carg16486-RA vs. Swiss-Prot
Match: sp|O22763|BZP10_ARATH (Basic leucine zipper 10 OS=Arabidopsis thaliana OX=3702 GN=BZIP10 PE=1 SV=2)

HSP 1 Score: 129.0 bits (323), Expect = 1.1e-28
Identity = 108/334 (32.34%), Postives = 157/334 (47.01%), Query Frame = 0


Query: 4   MDRVFSVDGIADQFLSSPPRAVPTEPEES-----SKLNRSASEWSFRRFLQEASVSXXXX 63
           M+ +FS+D  +D F  +PP  +P  P+ S      ++++S  EW+F  FL+E S S    
Sbjct: 1   MNSIFSIDDFSDPFWETPP--IPLNPDSSKPVTADEVSQSQPEWTFEMFLEEISSS---- 60

Query: 64  XXXXXXXXXXXXXXXXXXXXXXNANQILQKQSNRNEICSSSSATSRTEKSKAAESVKIGP 123
                                 N N  +   S+   + S S      + S+  +      
Sbjct: 61  --------------AVSSEPLGNNNNAIVGVSSAQSLPSVSGQNDFEDDSRFRDRDSGNL 120

Query: 124 PCVPP--------DSGDYQAFLKSKLNLACAAVALCRINE-------------------- 183
            C  P        DS DY+  LK+KL   CA V   R+                      
Sbjct: 121 DCAAPMTTKTVIVDSDDYRRVLKNKLETECATVVSLRVGSVKPEDSTSSPETQLQPVQSS 180

Query: 184 -----PLGITSS-PCVQKKVGILGSTATISSSIEQTTDEEDDVVEGEDGTSERKDPAYAK 243
                 LG+TSS P   KK G+     T  SS E + DE+   ++ E+ T+    P   K
Sbjct: 181 PLTQGELGVTSSLPAEVKKTGVSMKQVTSGSSREYSDDED---LDEENETTGSLKPEDVK 240

Query: 244 RVRRMLSNRESARRSRKRKQAHLTELETQVAELRVENSALLKRFNDISQKYNESAVNNRV 299
           + RRMLSN               ++LETQV +L+ E+S+LLK+ ++++ KY+E+AV NR+
Sbjct: 241 KSRRMLSNXXXXXXXXXXXXXQTSDLETQVNDLKGEHSSLLKQLSNMNHKYDEAAVGNRI 300

[Close]

BLAST of Carg16486-RA vs. Swiss-Prot
Match: sp|Q9M1G6|BZP25_ARATH (Basic leucine zipper 25 OS=Arabidopsis thaliana OX=3702 GN=BZIP25 PE=1 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 1.3e-24
Identity = 106/324 (32.72%), Postives = 158/324 (48.77%), Query Frame = 0


Query: 35  LNRSASEWSFRRFLQEASVSXXXXXXXXXXXXXXXXXXXXXXXXXXNANQ---ILQKQSN 94
           + RS SEW+F R + E S S                          +  +    +QK  N
Sbjct: 40  MTRSQSEWAFHRLINELSGSDSSPTTNTIERSPPPVQSLSRLEETVDETEDVVEIQKPQN 99

Query: 95  RNEICSSSSATSRTEKSKAAESVKIGPPCVPPDSGDYQAFLKSKLNLACAAVALCRIN-- 154
              +       +R  ++ +++ V    P V  D   Y A LKSKL LACAAVA  R+   
Sbjct: 100 HRRLPVDDQGKNR-NRAPSSDPVDSSAPVV-VDPNQYHAILKSKLELACAAVAR-RVGTV 159

Query: 155 EPLGITSSPCVQKKV--GILGSTATISSSIE----QTTDEEDDVVEGEDGTSERKD---- 214
           +P   ++S   QK+    I+  T+  +SS+      +T ++ DV   +   S R D    
Sbjct: 160 KPEDSSASASNQKQAQGSIVAQTSPGASSVRFSPTTSTQKKPDVPARQTSISSRDDXXXX 219

Query: 215 ----------PAYAKRVRRMLSNRESARRSRKRKQAHLTELETQVAELRVENSALLKRFN 274
                     P   KR RRMLSNRESAR      Q  + E +TQV +LR E+S L+ R +
Sbjct: 220 XXXXXXXXXXPTDVKRARRMLSNRESARXXXXXXQEQMNEFDTQVGQLRAEHSTLINRLS 279

Query: 275 DISQKYNESAVNNRVLKADLETLKAKVQMAEETVKRITGSKPMFYGMSSEVLSMSMQSFD 333
           D++ KY+ +AV+NR+L+AD+ETL+ KV+MAEETVKR+TG  P+ +   +  +      F 
Sbjct: 280 DMNHKYDAAAVDNRILRADIETLRTKVKMAEETVKRVTGVNPLHWSRPNMGI-----PFS 339

[Close]

BLAST of Carg16486-RA vs. TrEMBL
Match: tr|A0A1S3BPV6|A0A1S3BPV6_CUCME (light-inducible protein CPRF2-like OS=Cucumis melo OX=3656 GN=LOC103492232 PE=4 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 1.8e-92
Identity = 241/411 (58.64%), Postives = 273/411 (66.42%), Query Frame = 0


Query: 4   MDRV-FSVDGIADQFLSSPPRAVPTEPEESSKLNRSASEWSFRRFLQE-ASVSXXXXXXX 63
           MDRV FSVDGI+DQF  S     P  PEESSKLNRSASEWSFRRFLQE ASVS       
Sbjct: 2   MDRVLFSVDGISDQFWPS-----PDPPEESSKLNRSASEWSFRRFLQEAASVSDSSISPP 61

Query: 64  XXXXXXXXXXXXXXXXXXXNANQILQKQSNRNEICSSSSATSRTEKSKAAESVKIGPPCV 123
                              N     +KQS RN      +     E+ K++          
Sbjct: 62  PASPSNAVEVKESGEKLKRN----WEKQSIRN------NGEIEEERMKSSGG-------- 121

Query: 124 PPDSGDYQAFLKSKLNLACAAVALCR-----------------------------INEPL 183
             DS +Y+AFLKSKLNLACAAVALCR                              +   
Sbjct: 122 --DSEEYRAFLKSKLNLACAAVALCRGSFRKNQDSCASSTLAQNESQASASHLPSQSPSK 181

Query: 184 GITSSPCVQKKVGILGSTATISSSIEQTTDEEDDVVEGEDGTSERKDPAYAKRVRRMLSN 243
           GI+ SPCVQKK GI  S+A ISSS EQ TDEEDD VEGE+  +E+ DPA AKR+RRMLSN
Sbjct: 182 GISCSPCVQKKDGIQVSSANISSSREQ-TDEEDD-VEGENDMNEQMDPASAKRIRRMLSN 241

Query: 244 RESARRSRKRKQAHLTELETQVAELRVENSALLKRFNDISQKYNESAVNNRVLKADLETL 303
           RESARRSRKRKQAHLTELET+VAELR+ENS LLKRF+DISQKYNE+AVNNRVLKADLETL
Sbjct: 242 RESARRSRKRKQAHLTELETKVAELRLENSTLLKRFSDISQKYNEAAVNNRVLKADLETL 301

Query: 304 KAKVQMAEETVKRITGSKPMFYGMSSEVLSMSMQSFDGSPSKTSRDAASNHAADISSADT 363
           +AKVQMAEETVKRITG+K MF+ M SEV S+S+QSF+GSPS+ S DA ++H ADISSA+ 
Sbjct: 302 RAKVQMAEETVKRITGTKSMFHAM-SEVSSISIQSFEGSPSEISTDAHNSHIADISSANI 361

Query: 364 QLNCVAKAATVSGKTMVKTGSLRRVASLERLQKRIRGSSGCCEPSGKGDQQ 384
           Q N   + ATV    M +T SLRRVASLE LQKRIRG+S  C PSGKGDQQ
Sbjct: 362 QKNS-PEMATVPRNKMARTASLRRVASLEHLQKRIRGNSSTCHPSGKGDQQ 383

[Close]

BLAST of Carg16486-RA vs. TrEMBL
Match: tr|A0A0A0M1N8|A0A0A0M1N8_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G573650 PE=4 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 1.6e-88
Identity = 235/410 (57.32%), Postives = 267/410 (65.12%), Query Frame = 0


Query: 4   MDRV-FSVDGIADQFLSSPPRAVPTEPEESSKLNRSASEWSFRRFLQE-ASVSXXXXXXX 63
           MDRV FSVDGI+DQF  S        PEESSKLNRSASEWSFRRFLQE ASVS       
Sbjct: 2   MDRVLFSVDGISDQFWPS-----QDPPEESSKLNRSASEWSFRRFLQEAASVS------- 61

Query: 64  XXXXXXXXXXXXXXXXXXXNANQILQKQSNRNEICSSSSATSRTEKSKAAESVKIGPPCV 123
                              + +      SN  EI  S     ++++              
Sbjct: 62  -----------------DSSVSPPPASPSNAVEIKESGERLKQSKEXXXXXXXXXXXXXX 121

Query: 124 PPDSG----DYQAFLKSKLNLACAAVALCR------------------------INEPLG 183
                    +Y+AFLKSKLNLACAAVA+CR                         +   G
Sbjct: 122 XXXXXXXXEEYRAFLKSKLNLACAAVAMCRGSFRKSRDSCASSTLAQNMSHLPSQSPSKG 181

Query: 184 ITSSPCVQKKVGILGSTATISSSIEQTTDEEDDVVEGEDGTSERKDPAYAKRVRRMLSNR 243
           I  SPCVQK+ GI  S+A ISSS EQ TDEEDD VEGE+  +E+ DPA AKR+RRMLSNR
Sbjct: 182 ICCSPCVQKRDGIQVSSANISSSREQ-TDEEDD-VEGENDMNEQMDPASAKRIRRMLSNR 241

Query: 244 ESARRSRKRKQAHLTELETQVAELRVENSALLKRFNDISQKYNESAVNNRVLKADLETLK 303
           ESARRSRKRKQAHLTELETQVAELR ENS LLKRF+DISQKYNE+AVNNRVLKADLETL+
Sbjct: 242 ESARRSRKRKQAHLTELETQVAELRHENSTLLKRFSDISQKYNEAAVNNRVLKADLETLR 301

Query: 304 AKVQMAEETVKRITGSKPMFYGMSSEVLSMSMQSFDGSPSKTSRDAASNHAADISSADTQ 363
           AKVQMAEETVKRITG+K MF+ M SEV S+S+QSF+GSPS+ S DA ++H ADISSA+ Q
Sbjct: 302 AKVQMAEETVKRITGTKSMFHAM-SEVSSISIQSFEGSPSEISTDAHNSHIADISSANIQ 361

Query: 364 LNCVAKAATVSGKTMVKTGSLRRVASLERLQKRIRGSSGCCEPSGKGDQQ 384
            N + + ATV    M +T SLRRVASLE LQKRIRGSS  C PSGKGDQQ
Sbjct: 362 KNSL-EMATVPRNKMARTASLRRVASLEHLQKRIRGSSSICHPSGKGDQQ 378

[Close]

BLAST of Carg16486-RA vs. TrEMBL
Match: tr|A0A2I4FF22|A0A2I4FF22_9ROSI (light-inducible protein CPRF2-like isoform X2 OS=Juglans regia OX=51240 GN=LOC108998199 PE=4 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 2.7e-72
Identity = 210/464 (45.26%), Postives = 254/464 (54.74%), Query Frame = 0


Query: 4   MDRVFSVDGIADQFLSSP--------PRAVPTEPEESSKLNRSASEWSFRRFLQ-EASVS 63
           MDRVFSVD I+DQF S P        P AV    EESS++NRS SEW+F+RFLQ EAS  
Sbjct: 1   MDRVFSVDEISDQFWSPPLRPPQPNLPTAVTGSSEESSRMNRSPSEWAFQRFLQLEASPK 60

Query: 64  XXXXXXXXXXXXXXXXXXXXXXXXXXNANQILQKQSNRNEICSSSSATSRTEKSKAAESV 123
                                       N +    +++N++  + + T+ +  +   +S 
Sbjct: 61  TDTSSADQHKNDTVFEIKEDDERNAPLGNHVQNAINDQNDVIQTKTTTNISSTTSFGDSC 120

Query: 124 KIG----PPCVPPDSGDYQAFLKSKLNLACAAVALCR----------------------- 183
                  PP +  DS +YQAFLKSKL+LACAAVAL R                       
Sbjct: 121 TASFNAPPPNIHADSEEYQAFLKSKLDLACAAVALTRGSLVKPQESAALAENGLQASNTS 180

Query: 184 ----------------------INEPLGITSSPCVQKKVGILGSTATISSSIEQTTDEED 243
                                  N PLGI S P VQKK GI     T  SS EQ+ DEE 
Sbjct: 181 QLGSQASSKGAGSDSSRSRDKDSNGPLGIPSLPAVQKKPGIPAKATTSGSSREQSDDEE- 240

Query: 244 DVVEGEDGTSERKDPAYAKRVRRMLSNRESARRSRKRKQAHLTELETQVAELRVENSALL 303
             VEGE+  SE  DPA AKRVRRMLSNRESARRSR+RKQAHLTELETQV++LRVENS+LL
Sbjct: 241 --VEGENELSENMDPADAKRVRRMLSNRESARRSRRRKQAHLTELETQVSQLRVENSSLL 300

Query: 304 KRFNDISQKYNESAVNNRVLKADLETLKAKVQMAEETVKRITGSKPMFYGMSSEVLSMSM 363
           KR  +ISQ YNE+AV+NRVLKAD+ETL+AKV+MAEE VKRITG  PMF  M S++ S+ M
Sbjct: 301 KRLTEISQNYNEAAVDNRVLKADVETLRAKVKMAEEMVKRITGLNPMFNAM-SDISSVGM 360

Query: 364 QSFDGSPSKTSRDAA---------------------------SNHAADISSAD-TQLNCV 382
            S+ GSPS TS DAA                           SN  ADISS +  Q N  
Sbjct: 361 PSYGGSPSDTSTDAAVPVQDNSXXXXXXXXXXXNISTHGLGVSNGLADISSVENVQKN-- 420

[Close]

BLAST of Carg16486-RA vs. TrEMBL
Match: tr|A0A2I4FF15|A0A2I4FF15_9ROSI (light-inducible protein CPRF2-like isoform X1 OS=Juglans regia OX=51240 GN=LOC108998199 PE=4 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 2.7e-72
Identity = 210/464 (45.26%), Postives = 254/464 (54.74%), Query Frame = 0


Query: 4   MDRVFSVDGIADQFLSSP--------PRAVPTEPEESSKLNRSASEWSFRRFLQ-EASVS 63
           MDRVFSVD I+DQF S P        P AV    EESS++NRS SEW+F+RFLQ EAS  
Sbjct: 1   MDRVFSVDEISDQFWSPPLRPPQPNLPTAVTGSSEESSRMNRSPSEWAFQRFLQLEASPK 60

Query: 64  XXXXXXXXXXXXXXXXXXXXXXXXXXNANQILQKQSNRNEICSSSSATSRTEKSKAAESV 123
                                       N +    +++N++  + + T+ +  +   +S 
Sbjct: 61  IDTSSADQHKNDTVFEIKEDDERNAPLGNHVQNAINDQNDVIQTKTTTNISSTTSFGDSC 120

Query: 124 KIG----PPCVPPDSGDYQAFLKSKLNLACAAVALCR----------------------- 183
                  PP +  DS +YQAFLKSKL+LACAAVAL R                       
Sbjct: 121 TASFNAPPPNIHADSEEYQAFLKSKLDLACAAVALTRGSLVKPQESAALAENGLQASNTS 180

Query: 184 ----------------------INEPLGITSSPCVQKKVGILGSTATISSSIEQTTDEED 243
                                  N PLGI S P VQKK GI     T  SS EQ+ DEE 
Sbjct: 181 QLGSQASSKGAGSDSSRSRDKDSNGPLGIPSLPAVQKKPGIPAKATTSGSSREQSDDEE- 240

Query: 244 DVVEGEDGTSERKDPAYAKRVRRMLSNRESARRSRKRKQAHLTELETQVAELRVENSALL 303
             VEGE+  SE  DPA AKRVRRMLSNRESARRSR+RKQAHLTELETQV++LRVENS+LL
Sbjct: 241 --VEGENELSENMDPADAKRVRRMLSNRESARRSRRRKQAHLTELETQVSQLRVENSSLL 300

Query: 304 KRFNDISQKYNESAVNNRVLKADLETLKAKVQMAEETVKRITGSKPMFYGMSSEVLSMSM 363
           KR  +ISQ YNE+AV+NRVLKAD+ETL+AKV+MAEE VKRITG  PMF  M S++ S+ M
Sbjct: 301 KRLTEISQNYNEAAVDNRVLKADVETLRAKVKMAEEMVKRITGLNPMFNAM-SDISSVGM 360

Query: 364 QSFDGSPSKTSRDAA---------------------------SNHAADISSAD-TQLNCV 382
            S+ GSPS TS DAA                           SN  ADISS +  Q N  
Sbjct: 361 PSYGGSPSDTSTDAAVPVQDNSXXXXXXXXXXXNISTHGLGVSNGLADISSVENVQKN-- 420

[Close]

BLAST of Carg16486-RA vs. TrEMBL
Match: tr|F6HTR1|F6HTR1_VITVI (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_14s0030g02200 PE=4 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 1.2e-67
Identity = 208/452 (46.02%), Postives = 250/452 (55.31%), Query Frame = 0


Query: 4   MDRVFSVDGIADQFLSSPPRAVPTEPEESSKLNRSASEWSFRRFLQEASVSXXXXXXXXX 63
           MDRVFSVD I +QF +SP    P+  +E S++NRSASEW+F+RFLQE+S           
Sbjct: 1   MDRVFSVDEIPEQFWASP----PSSSKEPSQMNRSASEWAFQRFLQESS----------- 60

Query: 64  XXXXXXXXXXXXXXXXXNANQILQKQSNRNEICSSSSATSRTEKSKAAESVKIGPPCVPP 123
                 XXXX         N +++ +   ++                       PP VP 
Sbjct: 61  ---SPPXXXXVSASGPSAENDVVELKVPIDD----PXXXXXXXXXXXXXXXXXPPPNVPI 120

Query: 124 DSGDYQAFLKSKLNLACAAVALCRIN---------------------------------- 183
           DS +YQAFLKS+LNLACAAVAL R +                                  
Sbjct: 121 DSEEYQAFLKSRLNLACAAVALSRASFVKPQDSAALADTGSQASKTSQLRSQAPCKGSGY 180

Query: 184 -----------EPLGITSSPCVQKKVGILGSTATISSSIEQTTDEEDDVVEGEDGTSERK 243
                       PLGI S P +QKK G      T  SS E +   +DD VEGE  T E  
Sbjct: 181 DLSGAPDKEAGGPLGIPSLPAMQKKAGAQLRPTTSESSREHS---DDDEVEGETETIENM 240

Query: 244 DPAYAKRVRRMLSNRESARRSRKRKQAHLTELETQVAELRVENSALLKRFNDISQKYNES 303
           DPA AKRVRRMLSNRESARRSR+RKQAHLTELETQVA+LR+ENS+LLKR  DISQKYNE+
Sbjct: 241 DPADAKRVRRMLSNRESARRSRRRKQAHLTELETQVAQLRLENSSLLKRLTDISQKYNEA 300

Query: 304 AVNNRVLKADLETLKAKVQMAEETVKRITGSKPMFYGMSSEVLSMSMQSFDGSPSKTSRD 363
           AV+NRVLKAD+ETL+AKV+MAEETVKR+TG  P+F  M SE+    M SFDGSPS TS D
Sbjct: 301 AVDNRVLKADVETLRAKVKMAEETVKRVTGLNPLFQTM-SEISMAGMHSFDGSPSDTSAD 360

Query: 364 AA---------------------------SNHAADISSADTQLNCVAKAATVSGKTMVKT 384
           AA                           +N  AD+SS ++ L   A AA  +G  M +T
Sbjct: 361 AAVPVQDEPKQHFYPSPSDNLISTHDPRINNGLADVSSVESVLQNPA-AAGAAGNKMGRT 420

The following BLAST results are available for this feature:

BLAST of Carg16486-RA vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023529858.1	5.5e-170	92.00	light-inducible protein CPRF2-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022926013.1	2.0e-167	90.82	light-inducible protein CPRF2-like isoform X1 [Cucurbita moschata]	[more]
XP_023002073.1	2.0e-164	89.11	light-inducible protein CPRF2-like isoform X1 [Cucurbita maxima]	[more]
XP_023529859.1	2.3e-163	92.69	light-inducible protein CPRF2-like isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_022926023.1	2.8e-161	92.41	light-inducible protein CPRF2-like isoform X2 [Cucurbita moschata]	[more]

BLAST of Carg16486-RA vs. TAIR10


Match Name	E-value	Identity	Description
AT5G28770.2	8.0e-46	41.28	bZIP transcription factor family protein	[more]
AT4G02640.2	3.0e-29	31.76	bZIP transcription factor family protein	[more]
AT3G54620.1	7.0e-26	32.72	basic leucine zipper 25	[more]
AT5G24800.1	3.7e-19	50.42	basic leucine zipper 9	[more]
AT5G15830.1	2.1e-06	36.67	basic leucine-zipper 3	[more]

BLAST of Carg16486-RA vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q99090\|CPRF2_PETCR	6.4e-61	41.81	Light-inducible protein CPRF2 OS=Petroselinum crispum OX=4043 GN=CPRF2 PE=2 SV=2	[more]
sp\|B9DGI8\|BZP63_ARATH	1.4e-44	41.28	Basic leucine zipper 63 OS=Arabidopsis thaliana OX=3702 GN=BZIP63 PE=1 SV=1	[more]
sp\|Q7X9A8\|RSBZ2_ORYSJ	6.3e-32	48.76	bZIP transcription factor RISBZ2 OS=Oryza sativa subsp. japonica OX=39947 GN=RIS...	[more]
sp\|O22763\|BZP10_ARATH	1.1e-28	32.34	Basic leucine zipper 10 OS=Arabidopsis thaliana OX=3702 GN=BZIP10 PE=1 SV=2	[more]
sp\|Q9M1G6\|BZP25_ARATH	1.3e-24	32.72	Basic leucine zipper 25 OS=Arabidopsis thaliana OX=3702 GN=BZIP25 PE=1 SV=1	[more]

BLAST of Carg16486-RA vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3BPV6\|A0A1S3BPV6_CUCME	1.8e-92	58.64	light-inducible protein CPRF2-like OS=Cucumis melo OX=3656 GN=LOC103492232 PE=4 ...	[more]
tr\|A0A0A0M1N8\|A0A0A0M1N8_CUCSA	1.6e-88	57.32	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G573650 PE=4 SV=1	[more]
tr\|A0A2I4FF22\|A0A2I4FF22_9ROSI	2.7e-72	45.26	light-inducible protein CPRF2-like isoform X2 OS=Juglans regia OX=51240 GN=LOC10...	[more]
tr\|A0A2I4FF15\|A0A2I4FF15_9ROSI	2.7e-72	45.26	light-inducible protein CPRF2-like isoform X1 OS=Juglans regia OX=51240 GN=LOC10...	[more]
tr\|F6HTR1\|F6HTR1_VITVI	1.2e-67	46.02	Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_14s0030g02200 PE=4 SV=...	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Biological Process**
Term	Definition
GO:0006355	regulation of transcription, DNA-templated

Vocabulary: **Molecular Function**
Term	Definition
GO:0003700	transcription factor activity, sequence-specific DNA binding

Vocabulary: **INTERPRO**
Term	Definition
IPR020983	Basic_leucine-zipper_C
IPR004827	bZIP

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005667	transcription factor complex
molecular_function	GO:0003700	transcription factor activity, sequence-specific DNA binding

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg16486	Carg16486	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg16486-RA	Carg16486-RA-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg16486-RA.exon.1	Carg16486-RA.exon.1	exon
Carg16486-RA.exon.2	Carg16486-RA.exon.2	exon
Carg16486-RA.exon.3	Carg16486-RA.exon.3	exon
Carg16486-RA.exon.4	Carg16486-RA.exon.4	exon
Carg16486-RA.exon.5	Carg16486-RA.exon.5	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg16486-RA.five_prime_UTR.1	Carg16486-RA.five_prime_UTR.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg16486-RA.CDS.1	Carg16486-RA.CDS.1	CDS
Carg16486-RA.CDS.2	Carg16486-RA.CDS.2	CDS
Carg16486-RA.CDS.3	Carg16486-RA.CDS.3	CDS
Carg16486-RA.CDS.4	Carg16486-RA.CDS.4	CDS
Carg16486-RA.CDS.5	Carg16486-RA.CDS.5	CDS

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 262..289
None	No IPR available	COILS	Coil	Coil	coord: 213..247
None	No IPR available	GENE3D	G3DSA:1.20.5.170		coord: 204..258 e-value: 3.6E-15 score: 57.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 177..198
None	No IPR available	PANTHER	PTHR22952:SF348	SUBFAMILY NOT NAMED	coord: 24..147 coord: 324..372 coord: 148..323
None	No IPR available	PANTHER	PTHR22952	CAMP-RESPONSE ELEMENT BINDING PROTEIN-RELATED	coord: 24..147 coord: 324..372 coord: 148..323
None	No IPR available	CDD	cd14702	bZIP_plant_GBF1	coord: 205..256 e-value: 3.84433E-18 score: 76.4185
None	No IPR available	SUPERFAMILY	SSF57959	Leucine zipper domain	coord: 204..254
IPR004827	Basic-leucine zipper domain	SMART	SM00338	brlzneu	coord: 196..264 e-value: 2.2E-16 score: 70.4
IPR004827	Basic-leucine zipper domain	PFAM	PF00170	bZIP_1	coord: 203..256 e-value: 2.6E-13 score: 49.8
IPR004827	Basic-leucine zipper domain	PROSITE	PS00036	BZIP_BASIC	coord: 207..222
IPR004827	Basic-leucine zipper domain	PROSITE	PS50217	BZIP	coord: 202..257 score: 11.174
IPR020983	Basic leucine-zipper, C-terminal	PFAM	PF12498	bZIP_C	coord: 271..320 e-value: 6.9E-13 score: 49.6 coord: 320..370 e-value: 4.3E-6 score: 27.6