CSPI04G12450.1 (mRNA) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI04G12450.1
Type	mRNA
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	RING/U-box superfamily protein
Location	Chr4 : 10884866 .. 10886923 (+)
Sequence length	1089

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTAAATAAGAAAATAACAATAACAAATAGTAAAATAAATGCAATAAAGGGAGAGAGAGAGAGAGAAAGAGAGTTTGAAGAAGCGGATAGGAAGGGTCGGGTTGTCCAGAGAAAGAGAGAGGGAGAGAGGATCTTTTTATTATTTACCTTTTTGTTGTTTATAACGTTGAATTCTCTCTCTCTCTCCTTCGCCACGGCCTCCTCTTATTCAATTCAATTCGCCGTTTCCTAGTCGGACCGACCGCACAGATTCCTGGAATTCCTCATCATACGCTTCGCTTTTAAGTAATTCCTTCTTCTTCTTTGTTTTCCTCCTCCCATCCTTCTTGCTATTGCTTGCTTTTTTTTTTTTTGTTCTTTCCACTTTTCATCCTTTTCTTTTGACGGTTGACCCTTTAACGGTGGATCTGGGATCTGTGTACGGTTTCCAAGTTTTTGCGCGTTCCACGTCTCACATCTGATCGGACGGATTCCCCCCCATCACCTGCCTACATGTACGGTTTGTGACCATTTTCTTACATTTCTACAGAAAACATAATACCGGAAGGCTGCGAGTGATCATCCCACTACTACAATACAATTTTTTGAGGAGACGATGGACGAGTTAATTGCAACCAGATATTGGTGTCACATGTGTTCTCAAATGGTGAATCCCATCGTTGACGTGGAAATTAAATGCCCCTTCTGCCGCAGCGGCTTTGTTGAGGAGATAGGAAATGGTATTTCCAGCGACAATAACGATAACAACAACAATAACAATAATAACAACGAGCTCGAATCCGATTTTGGGTCGGAGCGAGCTTTGTCTCTCTGGGCTCCGATCTTGCTGGGTATGATGGGAAATCCCCGCGGGCGACGAAGATTCAGACATCTAGAGTTTGACGACGACGATGATGATAATGAGCATGAGGATGGAGAGGGGAATCGTGTGAGTAGTGAGACGACTGAGCTTGATTCAGTCATTAGGAGGAGGAGAAGTTCAGCTACCATTCTTCAGTTGCTCCAAGGTATCAGGGCTGGAATTGCAACTGCTGAATCAGAGAATAGTTATGAGGGAGAGAGGAGCAGAGAAAGAGAGAGGGTTATTCTTATAAATCCCTTCAATCAGACCATTGTAGTTCAAGGAGGTGAGAATCAAAATCAAAACCAAAACTCCATTGGGTCGCTGGGTGATTATTTCGTAGGACCTGGTTTGGATTTGTTATTGCAACATATAGCCGAGAATGATCCCAACAGATATGGCACTCCACCAGCACAGAAGGAGGCAGTTGATGCGCTGCCCACCGTGAGGGTTGAACTTGAGGAGGATAGCTGTTTGCAATGTTCAGTGTGTTTGGATGAGTTTGAGGTGGATGAAGAAGCCAAGGAGATGCCATGTAAGCATAAATTTCATACAGGTTGTATTCTGCCATGGTTGGAGCTGCATAGTTCTTGCCCAGTTTGTAGGCATCAATTGCCTGGGGATGAATCCAAGCGTGATGTTGATGGTGGGTCTGCTGCGAGGTTCATGAGCGACCTCAATAATGGTAATGGCAATGGCAGCGGGGAGATTGAGGGGAGAAATTCATCTGAGTCTGGGAGGAGGTTTTCGTTTCCATGGCCTTTTAATGGCTTGTTTACTTCCCAAGGAAACTCCTCGGCTGGAAGTGCAAGTGATTCTCAAAGGGATCACACTTCCATTTGAATATCCTGCTTCTGCTCTGCGTGCTTGCTTATGTATGTGTATGTATGTATGTGTGTTATACTTATATTAATTATACTATACAGATGTTTTGCCTCCTGTTCTCAGTAGAACAATTTCTCTTCTTAATATTTTACCAATATTGTATATGCCTTTGCACCCTTATCTCTGCTTTTGTTTCTATTTCTTGACCTCCGAAAACACCTGCACCTTAAATTCAACTACAGATATCATCTCACTCTGTGTTTTGTGGCAGGGTCAACGATATTGGCTGCGTACTCGCCCATATATATGCGTGTCTGTAGGAATAATAACTTTCATCCCCAGTGATTTTGAAACATATAACGGGAATGATATTTGTAGGCTATGTAATTTTGA

mRNA sequence

ATGGACGAGTTAATTGCAACCAGATATTGGTGTCACATGTGTTCTCAAATGGTGAATCCCATCGTTGACGTGGAAATTAAATGCCCCTTCTGCCGCAGCGGCTTTGTTGAGGAGATAGGAAATGGTATTTCCAGCGACAATAACGATAACAACAACAATAACAATAATAACAACGAGCTCGAATCCGATTTTGGGTCGGAGCGAGCTTTGTCTCTCTGGGCTCCGATCTTGCTGGGTATGATGGGAAATCCCCGCGGGCGACGAAGATTCAGACATCTAGAGTTTGACGACGACGATGATGATAATGAGCATGAGGATGGAGAGGGGAATCGTGTGAGTAGTGAGACGACTGAGCTTGATTCAGTCATTAGGAGGAGGAGAAGTTCAGCTACCATTCTTCAGTTGCTCCAAGGTATCAGGGCTGGAATTGCAACTGCTGAATCAGAGAATAGTTATGAGGGAGAGAGGAGCAGAGAAAGAGAGAGGGTTATTCTTATAAATCCCTTCAATCAGACCATTGTAGTTCAAGGAGGTGAGAATCAAAATCAAAACCAAAACTCCATTGGGTCGCTGGGTGATTATTTCGTAGGACCTGGTTTGGATTTGTTATTGCAACATATAGCCGAGAATGATCCCAACAGATATGGCACTCCACCAGCACAGAAGGAGGCAGTTGATGCGCTGCCCACCGTGAGGGTTGAACTTGAGGAGGATAGCTGTTTGCAATGTTCAGTGTGTTTGGATGAGTTTGAGGTGGATGAAGAAGCCAAGGAGATGCCATGTAAGCATAAATTTCATACAGGTTGTATTCTGCCATGGTTGGAGCTGCATAGTTCTTGCCCAGTTTGTAGGCATCAATTGCCTGGGGATGAATCCAAGCGTGATGTTGATGGTGGGTCTGCTGCGAGGTTCATGAGCGACCTCAATAATGGTAATGGCAATGGCAGCGGGGAGATTGAGGGGAGAAATTCATCTGAGTCTGGGAGGAGGTTTTCGTTTCCATGGCCTTTTAATGGCTTGTTTACTTCCCAAGGAAACTCCTCGGCTGGAAGTGCAAGTGATTCTCAAAGGGATCACACTTCCATTTGA

Coding sequence (CDS)

ATGGACGAGTTAATTGCAACCAGATATTGGTGTCACATGTGTTCTCAAATGGTGAATCCCATCGTTGACGTGGAAATTAAATGCCCCTTCTGCCGCAGCGGCTTTGTTGAGGAGATAGGAAATGGTATTTCCAGCGACAATAACGATAACAACAACAATAACAATAATAACAACGAGCTCGAATCCGATTTTGGGTCGGAGCGAGCTTTGTCTCTCTGGGCTCCGATCTTGCTGGGTATGATGGGAAATCCCCGCGGGCGACGAAGATTCAGACATCTAGAGTTTGACGACGACGATGATGATAATGAGCATGAGGATGGAGAGGGGAATCGTGTGAGTAGTGAGACGACTGAGCTTGATTCAGTCATTAGGAGGAGGAGAAGTTCAGCTACCATTCTTCAGTTGCTCCAAGGTATCAGGGCTGGAATTGCAACTGCTGAATCAGAGAATAGTTATGAGGGAGAGAGGAGCAGAGAAAGAGAGAGGGTTATTCTTATAAATCCCTTCAATCAGACCATTGTAGTTCAAGGAGGTGAGAATCAAAATCAAAACCAAAACTCCATTGGGTCGCTGGGTGATTATTTCGTAGGACCTGGTTTGGATTTGTTATTGCAACATATAGCCGAGAATGATCCCAACAGATATGGCACTCCACCAGCACAGAAGGAGGCAGTTGATGCGCTGCCCACCGTGAGGGTTGAACTTGAGGAGGATAGCTGTTTGCAATGTTCAGTGTGTTTGGATGAGTTTGAGGTGGATGAAGAAGCCAAGGAGATGCCATGTAAGCATAAATTTCATACAGGTTGTATTCTGCCATGGTTGGAGCTGCATAGTTCTTGCCCAGTTTGTAGGCATCAATTGCCTGGGGATGAATCCAAGCGTGATGTTGATGGTGGGTCTGCTGCGAGGTTCATGAGCGACCTCAATAATGGTAATGGCAATGGCAGCGGGGAGATTGAGGGGAGAAATTCATCTGAGTCTGGGAGGAGGTTTTCGTTTCCATGGCCTTTTAATGGCTTGTTTACTTCCCAAGGAAACTCCTCGGCTGGAAGTGCAAGTGATTCTCAAAGGGATCACACTTCCATTTGA

Homology

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BLAST of CSPI04G12450.1 vs. Swiss-Prot
Match: RNG1L_ARATH (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 2.2e-39
Identity = 85/169 (50.30%), Postives = 111/169 (65.68%), Query Frame = 1


Query: 189 GSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRV--ELEEDSCLQCSVC 248
           G+ GDYF GPGL+ L+Q +AENDPNRYGTPPA K A+DALPTV+V  ++ +    QC+VC
Sbjct: 160 GNFGDYFFGPGLEQLIQQLAENDPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVC 219

Query: 249 LDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGSAARFMS 308
           +DEFE   + K+MPCKH FH  C+LPWLELH+SCPVCR +LP D+   D +  S      
Sbjct: 220 MDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPTDDP--DYENRSQG---- 279

Query: 309 DLNNGNGNGSGEIEGRNSSESGRRFS--FPWPFNGLFTSQGNSSAGSAS 354
             + G+G+G G +EG+ +     RFS   PWPF      Q  S +GS +
Sbjct: 280 --SQGSGDGQGSVEGQQTP----RFSIQLPWPFR----RQDGSGSGSGA 312

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BLAST of CSPI04G12450.1 vs. Swiss-Prot
Match: RING1_GOSHI (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum GN=RING1 PE=1 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 4.2e-38
Identity = 82/173 (47.40%), Postives = 108/173 (62.43%), Query Frame = 1


Query: 190 SLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEE--DSCLQCSVCL 249
           ++GDYF+GPGL+ L+Q +AENDPNRYGTPPA K A++ALP V +          QC+VC+
Sbjct: 169 NIGDYFIGPGLEQLIQQLAENDPNRYGTPPASKSAIEALPLVNITKSNLNSEFNQCAVCM 228

Query: 250 DEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDES--KRDVDGGSAARFM 309
           D+FE   EAK+MPCKH +H  C+LPWLELH+SCPVCRH+LP D+   +R V G   A+  
Sbjct: 229 DDFEEGTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHELPTDDPDYERRVRG---AQGT 288

Query: 310 SDLNNGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQRD 359
           S  N+G+ +G        + E   R S PWPF       G +   +A   Q D
Sbjct: 289 SGGNDGDNSGQRSDGDNRTVERSFRISLPWPFQA--RGPGPAPGDNAETRQED 336

[Close]

BLAST of CSPI04G12450.1 vs. Swiss-Prot
Match: RHC1A_ARATH (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana GN=RHC1A PE=2 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 4.1e-25
Identity = 67/166 (40.36%), Postives = 90/166 (54.22%), Query Frame = 1


Query: 189 GSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEE--DSCLQCSVC 248
           G+ GDYF GPGL+ L + ++     R G PPA + A+DALPT+++       S   C VC
Sbjct: 135 GNTGDYFFGPGLEELFEQLSAGTTRR-GPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVC 194

Query: 249 LDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGSAARFMS 308
            DEFE+  EAK+MPC H +H+ CI+PWL  H+SCPVCR +LP           S  R   
Sbjct: 195 KDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASG----PSSSQNRTTP 254

Query: 309 DLNNGNGNGSGEIEGR-NSSESGRRFSFPWPFNGLFTSQGNSSAGS 352
             N  + + S     R N +E    FS  WPF     S G+SS+ +
Sbjct: 255 TRNYRSSSSSSSSNSRENGNERRNPFSSFWPFR----SSGSSSSST 291

[Close]

BLAST of CSPI04G12450.1 vs. Swiss-Prot
Match: RN115_HUMAN (E3 ubiquitin-protein ligase RNF115 OS=Homo sapiens GN=RNF115 PE=1 SV=2)

HSP 1 Score: 107.5 bits (267), Expect = 3.2e-22
Identity = 53/121 (43.80%), Postives = 76/121 (62.81%), Query Frame = 1


Query: 192 GDYFVGP-GLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEE-DSCLQCSVCLDE 251
           GDY  G  GLD ++  +     N  G PPA KE + +LPTV V  E+ D  L+C VC ++
Sbjct: 176 GDYAWGQTGLDAIVTQLLGQLENT-GPPPADKEKITSLPTVTVTQEQVDMGLECPVCKED 235

Query: 252 FEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKR---DVDGGSAARFMS 308
           + V+EE +++PC H FH+ CI+PWLELH +CPVCR  L G++S R     +  ++ RF +
Sbjct: 236 YTVEEEVRQLPCNHFFHSSCIVPWLELHDTCPVCRKSLNGEDSTRQSQSTEASASNRFSN 295

[Close]

BLAST of CSPI04G12450.1 vs. Swiss-Prot
Match: RN115_MOUSE (E3 ubiquitin-protein ligase RNF115 OS=Mus musculus GN=Rnf115 PE=1 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 9.4e-22
Identity = 52/121 (42.98%), Postives = 76/121 (62.81%), Query Frame = 1


Query: 192 GDYFVGP-GLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEE-DSCLQCSVCLDE 251
           GDY  G  GLD ++  +     N  G PPA KE + +LPTV V  E+ ++ L+C VC ++
Sbjct: 177 GDYAWGQTGLDAIVTQLLGQLENT-GPPPADKEKITSLPTVTVTQEQVNTGLECPVCKED 236

Query: 252 FEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGSAA---RFMS 308
           + V+E+ +++PC H FH+ CI+PWLELH +CPVCR  L G++S R      A+   RF +
Sbjct: 237 YTVEEKVRQLPCNHFFHSSCIVPWLELHDTCPVCRKSLNGEDSTRQTQSSEASASNRFSN 296

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BLAST of CSPI04G12450.1 vs. TrEMBL
Match: A0A0A0KWR3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G267990 PE=4 SV=1)

HSP 1 Score: 729.9 bits (1883), Expect = 1.5e-207
Identity = 362/362 (100.00%), Postives = 362/362 (100.00%), Query Frame = 1


Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60
           MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL
Sbjct: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELD 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELD
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELD 120

Query: 121 SVIRRRRSSATILQLLQGIRAGIATAESENSYEGERSRERERVILINPFNQTIVVQGGEN 180
           SVIRRRRSSATILQLLQGIRAGIATAESENSYEGERSRERERVILINPFNQTIVVQGGEN
Sbjct: 121 SVIRRRRSSATILQLLQGIRAGIATAESENSYEGERSRERERVILINPFNQTIVVQGGEN 180

Query: 181 QNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSC 240
           QNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSC
Sbjct: 181 QNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSC 240

Query: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300
           LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS
Sbjct: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300

Query: 301 AARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQRDHT 360
           AARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQRDHT
Sbjct: 301 AARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQRDHT 360

Query: 361 SI 363
           SI
Sbjct: 361 SI 362

[Close]

BLAST of CSPI04G12450.1 vs. TrEMBL
Match: W9S1Q0_9ROSA (E3 ubiquitin-protein ligase RING1 OS=Morus notabilis GN=L484_002344 PE=4 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 2.0e-124
Identity = 244/369 (66.12%), Postives = 287/369 (77.78%), Query Frame = 1


Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60
           M+E IA+RYWCHMCSQ VNPI++VEIKCPFC+SGFVEE+ +  SS+NN N     N +  
Sbjct: 1   MEETIASRYWCHMCSQEVNPIMEVEIKCPFCQSGFVEEMSSIDSSNNNPNPEGGRNADFS 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETT--- 120
           +SDFGS+RALSLWAPILLGMMG+PR RRRFR L+FDD DD +  +DGE      ET    
Sbjct: 61  DSDFGSDRALSLWAPILLGMMGHPRRRRRFRRLDFDDYDDHDVVDDGESEDHGGETELDR 120

Query: 121 ELDSVIRRRR-SSATILQLLQGIRAGIATAESENSY---EGERSR-ERERVILINPFNQT 180
           EL+S+IRRRR SSATILQLLQGIRAG+A+ ES+NS    +G+R R ERERVILINPFNQT
Sbjct: 121 ELESIIRRRRRSSATILQLLQGIRAGLAS-ESQNSESFRDGDRERDERERVILINPFNQT 180

Query: 181 IVVQGGENQNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVR 240
           ++VQG   QN+NQN IGSLGDYF+GPGLD+LLQH+AENDPNRYGTPPAQKEAV+ALPTV 
Sbjct: 181 VLVQG---QNENQNPIGSLGDYFIGPGLDMLLQHLAENDPNRYGTPPAQKEAVEALPTVA 240

Query: 241 VELEEDSCLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDES 300
           ++      LQCSVCLD+FE+  +AKEMPCKHKFH GCILPWLELHSSCPVCR QLP +ES
Sbjct: 241 IK----ENLQCSVCLDDFEIGADAKEMPCKHKFHNGCILPWLELHSSCPVCRFQLPSNES 300

Query: 301 KRDVDG------GSAARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGN 356
           KRD DG        A   +S  NN + NG  E +G   + SGRRFSFPWPF+GLF+S G+
Sbjct: 301 KRDSDGTRNANVERAMNMISSENNSSANGGDEGDGDGRNGSGRRFSFPWPFSGLFSSSGS 360

[Close]

BLAST of CSPI04G12450.1 vs. TrEMBL
Match: M5VYQ4_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007335mg PE=4 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 1.3e-115
Identity = 230/368 (62.50%), Postives = 277/368 (75.27%), Query Frame = 1


Query: 8   RYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNELESDFGSE 67
           RYWCH+CSQMV+PI++VE+KCPFC+SGF+EE+           N+N  +++E +SDFGS+
Sbjct: 5   RYWCHVCSQMVDPIMEVEMKCPFCQSGFIEEM-----------NSNTRDSHEADSDFGSD 64

Query: 68  RALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETT---ELDSVIR 127
           RALSLWAPILLGMM NP  RRR R L+FD+D+DD +++DGE  R   +T    EL+S+IR
Sbjct: 65  RALSLWAPILLGMMNNPHRRRRLRRLDFDEDEDD-DNDDGEA-RHGGDTELDRELESIIR 124

Query: 128 RRR-SSATILQLLQGIRAGIATAESENSY-----------EGERSRERERVILINPFNQT 187
           RRR +SATILQLLQGIRAG A+ ESENS            E ER RERERVILINPF+QT
Sbjct: 125 RRRRNSATILQLLQGIRAGSAS-ESENSESDRDRDRDRDRERERERERERVILINPFSQT 184

Query: 188 IVVQGGENQNQNQNS----IGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDAL 247
           I+VQG  + NQ QN+    +GSLGDYF+GPGLDLLLQH+AENDPNRYGTPPAQKEAV+AL
Sbjct: 185 IIVQGSYDSNQGQNNNHTPMGSLGDYFIGPGLDLLLQHLAENDPNRYGTPPAQKEAVEAL 244

Query: 248 PTVRVELEEDSCLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLP 307
           PTV ++      LQCSVCLD+FE   EAKEMPCKHKFH+GCILPWLELHSSCPVCR QLP
Sbjct: 245 PTVTIKEN----LQCSVCLDDFEAGVEAKEMPCKHKFHSGCILPWLELHSSCPVCRFQLP 304

Query: 308 GDESKRDVDGGSAARFMSDLNNGNGNGSG-EIEGRNSSESGRRFSFPWPFNGLFTSQGNS 356
            DESKRD D    +    +  N  G+G G E +G   + SGRRFS PWPFNGLF++ G+ 
Sbjct: 305 ADESKRDSDNSRNSSDQRESENIGGDGGGVEGDGDGRNGSGRRFSIPWPFNGLFSNSGSQ 354

[Close]

BLAST of CSPI04G12450.1 vs. TrEMBL
Match: A0A061GPC3_THECC (RING/U-box superfamily protein isoform 1 OS=Theobroma cacao GN=TCM_038630 PE=4 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 1.1e-114
Identity = 222/369 (60.16%), Postives = 273/369 (73.98%), Query Frame = 1


Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60
           M+E +A RYWCH CS++VNPI++VEIKCP C +GF+EE+ +G             ++ ++
Sbjct: 1   MEEAMAARYWCHQCSRIVNPIMEVEIKCPVCETGFIEEMSSG-----------TRDSEDI 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELD 120
           +SD GS+RALSLWAPILLGMMGNPR RRR R ++F+++DDDN+  +      +    EL+
Sbjct: 61  DSDIGSDRALSLWAPILLGMMGNPRRRRRHRRIDFEEEDDDNDDGEARHGGDTDLDRELE 120

Query: 121 SVIRRRR-SSATILQLLQGIRAGIATAESENSYEG-------ERSRERERVILINPFNQT 180
           S+IRRRR SSATILQLLQGIR G+A+ E+ENS          +R RERERVILINPFNQT
Sbjct: 121 SIIRRRRRSSATILQLLQGIRDGMAS-EAENSENDRDRDTSRDRDRERERVILINPFNQT 180

Query: 181 IVVQGGEN-----QNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDA 240
           I+VQG  +     QNQN N IGSLGDYFVGPGLDLLLQH+AENDPNRYGTPPAQKEA++A
Sbjct: 181 IIVQGSYDSNQGGQNQNSNHIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAIEA 240

Query: 241 LPTVRVELEEDSCLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQL 300
           +PTV++E      LQCSVCLD+FE   EA+EMPCKHKFH+GCILPWLELHSSCPVCR+Q+
Sbjct: 241 MPTVKIEEN----LQCSVCLDDFEAGSEAREMPCKHKFHSGCILPWLELHSSCPVCRYQM 300

Query: 301 PGDESKRDVDGGSAARFMSDLNNGNGNGSGEI-EGRNSSESGRRFSFPWPFNGLFTSQGN 356
           P DESK D +         +  N N +GSGE  EG   S SGRRFSFPWPFNGLF+S   
Sbjct: 301 PADESKLDTERPRNNTNQRESEN-NVHGSGEEGEGDGRSGSGRRFSFPWPFNGLFSSGSQ 352

[Close]

BLAST of CSPI04G12450.1 vs. TrEMBL
Match: F6GUP5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_06s0004g00120 PE=4 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 3.4e-111
Identity = 221/371 (59.57%), Postives = 269/371 (72.51%), Query Frame = 1


Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60
           M++ +  RYWCH CSQ+VNPI + E KCP C+ GF+E+I    S D+ND+          
Sbjct: 1   MEDAMGGRYWCHSCSQVVNPIAEAEPKCPLCQDGFIEDIDGTASRDHNDS---------- 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETT--- 120
           +SD  S+R LSLWAPILLGMMGNPR RRRFR  + +D+++DNE  DGEG R   ET    
Sbjct: 61  DSDLASDRTLSLWAPILLGMMGNPRRRRRFRREDIEDEEEDNE--DGEGRR-GGETELDR 120

Query: 121 ELDSVIRRRR-SSATILQLLQGIRAGIATAESENSY-------EGERSRERERVILINPF 180
           ELDS+IRRRR SSATILQLLQGIRAG+ + ESENS        + ER RERER+ILINPF
Sbjct: 121 ELDSIIRRRRRSSATILQLLQGIRAGMVS-ESENSENDRDRDRDRERERERERLILINPF 180

Query: 181 NQTIVVQGG----ENQNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAV 240
           NQTI+VQG     + QNQNQN+IGSLGDYF+GP LDLLLQH+AENDPNRYGTPPAQK+A+
Sbjct: 181 NQTIIVQGSFDSNQAQNQNQNTIGSLGDYFIGPSLDLLLQHLAENDPNRYGTPPAQKDAI 240

Query: 241 DALPTVRVELEEDSCLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRH 300
           +A+PTV+++      LQCSVCLD+FE+  EA+EMPCKHKFH+GCILPWLELHSSCPVCR 
Sbjct: 241 EAMPTVKIKEN----LQCSVCLDDFEIGAEAREMPCKHKFHSGCILPWLELHSSCPVCRF 300

Query: 301 QLPGDESKRDVDGGSAARFMSDLNNGNGNGSGEI-EGRNSSESGRRFSFPWPFNGLFTSQ 356
           Q+  DESK D D        S   + NG+GS E  +G   S +GRR +F WPF  LF+SQ
Sbjct: 301 QIAADESKLDSDASPNVSSNSSERSNNGHGSSEEGDGDGRSGNGRRSTFRWPFTSLFSSQ 353

[Close]

BLAST of CSPI04G12450.1 vs. TAIR10
Match: AT5G56340.1 (AT5G56340.1 RING/U-box superfamily protein)

HSP 1 Score: 376.7 bits (966), Expect = 1.6e-104
Identity = 213/392 (54.34%), Postives = 265/392 (67.60%), Query Frame = 1


Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEI-GNGISSDNNDNNNNNNNNNE 60
           M+E +A RYWCHMCSQMVNP+++ EIKCPFC+SGF+EE+ GNG             +  +
Sbjct: 1   MEETMAARYWCHMCSQMVNPVMESEIKCPFCQSGFIEEMSGNG----GGGGGRGIRDVQD 60

Query: 61  LESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHE-----DGEGNRVSS 120
            E+DFG++RALSLWAPILLGMM +PR RRRFR  EF +++DDN  +     DG GN  ++
Sbjct: 61  SETDFGTDRALSLWAPILLGMMSSPRRRRRFRRSEFGEENDDNGDDLTTNADGNGNDSNN 120

Query: 121 ETT-------------------ELDSVIRRRR-SSATILQLLQGIRAGIATAESENSYEG 180
                                 E +S++RRRR SS  ILQLLQGIRAGIA+    +    
Sbjct: 121 SNNNVYRHHRARRHGGEIDLDREFESILRRRRRSSGNILQLLQGIRAGIASEYESSDNNW 180

Query: 181 ERSRERERVILINPFNQTIVVQGGENQNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNR 240
           + SRER+RVI+INP+NQ++VV    +QNQN  S+ SLGDYF+GPGLDLLLQH+AENDPNR
Sbjct: 181 DNSRERDRVIMINPYNQSLVVPS--DQNQNHPSLTSLGDYFIGPGLDLLLQHLAENDPNR 240

Query: 241 YGTPPAQKEAVDALPTVRVELEEDSCLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWL 300
            GTPPA+KEAV+ALPTV++       LQCSVCLD+FE   EAKEMPCKHKFH  CI+PWL
Sbjct: 241 QGTPPARKEAVEALPTVKIM----EPLQCSVCLDDFEKGTEAKEMPCKHKFHVRCIVPWL 300

Query: 301 ELHSSCPVCRHQLPG-----DESKRDVDGGSAARFMSDLNNGN-----GNGSGEIEGRNS 357
           ELHSSCPVCR +LP      DE+K D +     R + + +NGN     GN     E    
Sbjct: 301 ELHSSCPVCRFELPSSADDDDETKTDSERVLRTRNVRETSNGNVVENVGNADRGREDEVR 360

[Close]

BLAST of CSPI04G12450.1 vs. TAIR10
Match: AT4G26400.2 (AT4G26400.2 RING/U-box superfamily protein)

HSP 1 Score: 323.9 bits (829), Expect = 1.2e-88
Identity = 192/380 (50.53%), Postives = 247/380 (65.00%), Query Frame = 1


Query: 5   IATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNELESDF 64
           ++ RYWCHMCSQMVNP++  EIKCPFC+SGFVEE+   I   N  ++++     + E DF
Sbjct: 1   MSARYWCHMCSQMVNPVIGAEIKCPFCQSGFVEEMSREI---NGGSSSSLREVQDPEIDF 60

Query: 65  GSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDD-NEHEDGEGN------------- 124
           G++RALSLW PILLGMM NPR RRRFR  EF  D+D+ N  +D +GN             
Sbjct: 61  GTDRALSLWGPILLGMMSNPRRRRRFRRTEFGVDNDEVNVSDDVDGNDSVDIDRHHHRRH 120

Query: 125 ------RVSSETTELDSVIRRRR-SSATILQLLQGIRAGIATAESENSYEGERSRERERV 184
                 R      E +S++RRRR SSATILQLLQGIRAGIA+ E E+S         +R 
Sbjct: 121 RHRQQGREIDLDREFESILRRRRRSSATILQLLQGIRAGIAS-EYESS---------DRD 180

Query: 185 ILINPFNQTIVVQGGE--NQNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQ 244
           +L    NQ+ VVQG    NQN+N  S+ ++GDYFVG  LD LL+H+A+ND  R+G+ PA+
Sbjct: 181 LL----NQSAVVQGSTSLNQNRNNTSLSAIGDYFVGSSLDHLLEHLADNDSIRHGSLPAR 240

Query: 245 KEAVDALPTVRVELEEDSCLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCP 304
           KE VD LPTV++       LQCS+CLD+F+   EAKEMPCKHKFH  CI+PWLELHSSCP
Sbjct: 241 KEVVDNLPTVKIS----ESLQCSICLDDFDKGSEAKEMPCKHKFHIRCIVPWLELHSSCP 300

Query: 305 VCRHQLPGDESKRDVDGGSAARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPFNGLF 362
           VCR++LP D+  +        R + ++N  N N   E + RNS+ S RRFSFPWPF+GLF
Sbjct: 301 VCRYELPPDDETKVNPVRPRTRTL-EINVSNEN--VEDDARNSNVSERRFSFPWPFSGLF 356

[Close]

BLAST of CSPI04G12450.1 vs. TAIR10
Match: AT1G55530.1 (AT1G55530.1 RING/U-box superfamily protein)

HSP 1 Score: 304.7 bits (779), Expect = 7.7e-83
Identity = 182/375 (48.53%), Postives = 237/375 (63.20%), Query Frame = 1


Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60
           M+E + TRYWCHMCSQ VNP+++ EIKCPFC+SGFVEE+ +    D++D  +   NN   
Sbjct: 1   MEEAVVTRYWCHMCSQTVNPVMEAEIKCPFCQSGFVEEMEDDDDHDSSDPADVRANN--- 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDD-----DNEHEDGEGNRVSSE 120
                     SLWAPIL+ +M +P  RRR + +E  +D+      +N  +DGE N +  +
Sbjct: 61  ----------SLWAPILMELMNDPVRRRRNQSVESVEDNQNEVQTENNEDDGE-NDLDWQ 120

Query: 121 TTELDSVIRRRRSSATILQLLQGIRAGIATAESENSYEGERSRERERVILINPFNQTIVV 180
             E+  + RRRR SA +LQLLQGIRAG+ + ESE++  G       RVILIN  NQTI V
Sbjct: 121 LQEI--LRRRRRHSAAVLQLLQGIRAGL-SVESEST--GNGGNNPGRVILINTSNQTITV 180

Query: 181 QGGENQNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVEL 240
           Q   + + +    GSLGDYF+GPG ++LLQ +AENDPNRYGTPPA+KEAV+AL TV++E 
Sbjct: 181 Q--NSADMDSVPAGSLGDYFIGPGFEMLLQRLAENDPNRYGTPPAKKEAVEALATVKIE- 240

Query: 241 EEDSCLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRD 300
                LQCSVCLD+FE+  EAK MPC HKFH+ C+LPWLELHSSCPVCR+QLP DE+K D
Sbjct: 241 ---ETLQCSVCLDDFEIGTEAKLMPCTHKFHSDCLLPWLELHSSCPVCRYQLPADEAKTD 300

Query: 301 V-------DGGSAARFMSD--LNNGNGNGSGEIEGRNSSESGRR--------FSFPWPFN 354
                   +G S+A   +     N +GN   E E     E            FS PWPF+
Sbjct: 301 SVTTTSDNNGSSSASATTSHGAENSDGNRRQEEEEEEEEEEEEENENNDGSGFSIPWPFS 350

[Close]

BLAST of CSPI04G12450.1 vs. TAIR10
Match: AT3G13430.1 (AT3G13430.1 RING/U-box superfamily protein)

HSP 1 Score: 248.8 bits (634), Expect = 5.0e-66
Identity = 158/340 (46.47%), Postives = 204/340 (60.00%), Query Frame = 1


Query: 1   MDELIATRYWCHMCSQMVNPIVDVE-IKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNE 60
           M++   T YWCHMCS+ V P++  E IKC FC+SGFVEE+      DNND++        
Sbjct: 1   MEDASETSYWCHMCSRSVIPLIQDEIIKCNFCQSGFVEEM------DNNDDH-------- 60

Query: 61  LESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEF-----DDDDDDNEHEDGEGNRVS- 120
                  + A SLW PIL+ MM N   +      +      D+D+D+++ +DG+ N    
Sbjct: 61  -------QAADSLWTPILMEMMNNNHDQHSTNQEDSESILEDEDEDEDDGDDGDQNNDGE 120

Query: 121 -SETTELDSVIR-RRRSSATILQLLQGIRAGIATAESENSYEGERSRERERVILINPFNQ 180
              T +L+ + R R R S  I+ LLQGIRAG+   ESEN+ +   +   E V+LIN FNQ
Sbjct: 121 IDITHQLEEIRRIRTRHSTAIVNLLQGIRAGL-LIESENNEDNPDN--SELVVLINSFNQ 180

Query: 181 TIVVQGGENQNQNQNSIGSLGDYFVGPGLDLLLQHIAEND-PNRYGTPPAQKEAVDALPT 240
            I V        +  S GSLGDYF+GPG + LLQ +AEND  NRYGTPPA KEAV+AL  
Sbjct: 181 RIRVHQDSVDTTSVPS-GSLGDYFIGPGFETLLQRLAENDLNNRYGTPPATKEAVEALAM 240

Query: 241 VRVELEEDSCLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLP-- 300
           V++   EDS LQCSVCLD+FE+  EAKEMPCKHKFH+ C+LPWLELHSSCPVCR+ LP  
Sbjct: 241 VKI---EDSLLQCSVCLDDFEIGMEAKEMPCKHKFHSDCLLPWLELHSSCPVCRYLLPTG 300

Query: 301 -GDESKRDVDGGSAARFMSDLNNGN--GNGSGEIEGRNSS 326
             DE K D +         D++N +   NGS      N+S
Sbjct: 301 DDDEPKTDAETSRNDDNNEDISNASMASNGSSPDSSSNNS 312

[Close]

BLAST of CSPI04G12450.1 vs. TAIR10
Match: AT3G19950.1 (AT3G19950.1 RING/U-box superfamily protein)

HSP 1 Score: 164.5 bits (415), Expect = 1.2e-40
Identity = 85/169 (50.30%), Postives = 111/169 (65.68%), Query Frame = 1


Query: 189 GSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRV--ELEEDSCLQCSVC 248
           G+ GDYF GPGL+ L+Q +AENDPNRYGTPPA K A+DALPTV+V  ++ +    QC+VC
Sbjct: 160 GNFGDYFFGPGLEQLIQQLAENDPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVC 219

Query: 249 LDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGSAARFMS 308
           +DEFE   + K+MPCKH FH  C+LPWLELH+SCPVCR +LP D+   D +  S      
Sbjct: 220 MDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPTDDP--DYENRSQG---- 279

Query: 309 DLNNGNGNGSGEIEGRNSSESGRRFS--FPWPFNGLFTSQGNSSAGSAS 354
             + G+G+G G +EG+ +     RFS   PWPF      Q  S +GS +
Sbjct: 280 --SQGSGDGQGSVEGQQTP----RFSIQLPWPFR----RQDGSGSGSGA 312

[Close]

BLAST of CSPI04G12450.1 vs. NCBI nr
Match: gi|449463838|ref|XP_004149638.1| (PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis sativus])

HSP 1 Score: 729.9 bits (1883), Expect = 2.1e-207
Identity = 362/362 (100.00%), Postives = 362/362 (100.00%), Query Frame = 1


Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60
           MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL
Sbjct: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELD 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELD
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELD 120

Query: 121 SVIRRRRSSATILQLLQGIRAGIATAESENSYEGERSRERERVILINPFNQTIVVQGGEN 180
           SVIRRRRSSATILQLLQGIRAGIATAESENSYEGERSRERERVILINPFNQTIVVQGGEN
Sbjct: 121 SVIRRRRSSATILQLLQGIRAGIATAESENSYEGERSRERERVILINPFNQTIVVQGGEN 180

Query: 181 QNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSC 240
           QNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSC
Sbjct: 181 QNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSC 240

Query: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300
           LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS
Sbjct: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300

Query: 301 AARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQRDHT 360
           AARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQRDHT
Sbjct: 301 AARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQRDHT 360

Query: 361 SI 363
           SI
Sbjct: 361 SI 362

[Close]

BLAST of CSPI04G12450.1 vs. NCBI nr
Match: gi|659097967|ref|XP_008449904.1| (PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo])

HSP 1 Score: 712.6 bits (1838), Expect = 3.5e-202
Identity = 358/366 (97.81%), Postives = 358/366 (97.81%), Query Frame = 1


Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60
           MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL
Sbjct: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELD 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGN VSSETTELD
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNHVSSETTELD 120

Query: 121 SVIRRRRSSATILQLLQGIRAGIATAESENSYEGERSRERERVILINPFNQTIVVQGGEN 180
           SVIRRRRSSATILQLLQGIRAGIATAESENSYEGERSRERERVILINPFNQTIVVQGGEN
Sbjct: 121 SVIRRRRSSATILQLLQGIRAGIATAESENSYEGERSRERERVILINPFNQTIVVQGGEN 180

Query: 181 QNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSC 240
           QNQN NSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDS 
Sbjct: 181 QNQNHNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSR 240

Query: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300
           LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS
Sbjct: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300

Query: 301 AARFMSDLN----NGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ 360
           AARFMSDLN    NGNGNGSGEIE RNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ
Sbjct: 301 AARFMSDLNNGNGNGNGNGSGEIEERNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ 360

Query: 361 RDHTSI 363
           RDHTSI
Sbjct: 361 RDHTSI 366

[Close]

BLAST of CSPI04G12450.1 vs. NCBI nr
Match: gi|703133141|ref|XP_010105294.1| (E3 ubiquitin-protein ligase RING1 [Morus notabilis])

HSP 1 Score: 453.8 bits (1166), Expect = 2.9e-124
Identity = 244/369 (66.12%), Postives = 287/369 (77.78%), Query Frame = 1


Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60
           M+E IA+RYWCHMCSQ VNPI++VEIKCPFC+SGFVEE+ +  SS+NN N     N +  
Sbjct: 1   MEETIASRYWCHMCSQEVNPIMEVEIKCPFCQSGFVEEMSSIDSSNNNPNPEGGRNADFS 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETT--- 120
           +SDFGS+RALSLWAPILLGMMG+PR RRRFR L+FDD DD +  +DGE      ET    
Sbjct: 61  DSDFGSDRALSLWAPILLGMMGHPRRRRRFRRLDFDDYDDHDVVDDGESEDHGGETELDR 120

Query: 121 ELDSVIRRRR-SSATILQLLQGIRAGIATAESENSY---EGERSR-ERERVILINPFNQT 180
           EL+S+IRRRR SSATILQLLQGIRAG+A+ ES+NS    +G+R R ERERVILINPFNQT
Sbjct: 121 ELESIIRRRRRSSATILQLLQGIRAGLAS-ESQNSESFRDGDRERDERERVILINPFNQT 180

Query: 181 IVVQGGENQNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVR 240
           ++VQG   QN+NQN IGSLGDYF+GPGLD+LLQH+AENDPNRYGTPPAQKEAV+ALPTV 
Sbjct: 181 VLVQG---QNENQNPIGSLGDYFIGPGLDMLLQHLAENDPNRYGTPPAQKEAVEALPTVA 240

Query: 241 VELEEDSCLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDES 300
           ++      LQCSVCLD+FE+  +AKEMPCKHKFH GCILPWLELHSSCPVCR QLP +ES
Sbjct: 241 IK----ENLQCSVCLDDFEIGADAKEMPCKHKFHNGCILPWLELHSSCPVCRFQLPSNES 300

Query: 301 KRDVDG------GSAARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGN 356
           KRD DG        A   +S  NN + NG  E +G   + SGRRFSFPWPF+GLF+S G+
Sbjct: 301 KRDSDGTRNANVERAMNMISSENNSSANGGDEGDGDGRNGSGRRFSFPWPFSGLFSSSGS 360

[Close]

BLAST of CSPI04G12450.1 vs. NCBI nr
Match: gi|657951119|ref|XP_008351262.1| (PREDICTED: E3 ubiquitin-protein ligase CIP8-like [Malus domestica])

HSP 1 Score: 433.3 bits (1113), Expect = 4.1e-118
Identity = 235/379 (62.01%), Postives = 281/379 (74.14%), Query Frame = 1


Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60
           MDE +A RYWCH CS+MV+PI++VE+KCPFC+SGF+EE+ NG + +N D           
Sbjct: 1   MDEAMAARYWCHRCSRMVDPIMEVEMKCPFCQSGFIEEM-NGTTRENQD----------A 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDD-NEHEDGEGNRVSSETTEL 120
           +SD GS+RALSLWAPILLGMM NP  RRR R  +FDDDDDD N++++ +G+      TEL
Sbjct: 61  DSDLGSDRALSLWAPILLGMMNNPHRRRRLRRFDFDDDDDDDNDNDNDDGDTRQGGDTEL 120

Query: 121 D----SVIRRRR-SSATILQLLQGIRAGIATAESENSY-----EGERSRERERVILINPF 180
           D    S+IRRRR SSATILQLLQGIRAG+A+ ESENS      + ER RERERVILINPF
Sbjct: 121 DRELESIIRRRRRSSATILQLLQGIRAGLAS-ESENSEGDRERDRERERERERVILINPF 180

Query: 181 NQTIVVQGGENQNQNQNS----IGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAV 240
            QTI+VQG  + NQ QN+    IGSLGDY++GPGLDLLLQH+AENDPNRYGTPPAQKEAV
Sbjct: 181 XQTIIVQGSYDSNQGQNNNHTPIGSLGDYYIGPGLDLLLQHLAENDPNRYGTPPAQKEAV 240

Query: 241 DALPTVRVELEEDSCLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRH 300
           +ALPTV ++      LQCSVCLD+FEV  EAKEMPCKHKFH+GCILPWLELHSSCPVCR 
Sbjct: 241 EALPTVTIK----ENLQCSVCLDDFEVGXEAKEMPCKHKFHSGCILPWLELHSSCPVCRF 300

Query: 301 QLPGDESKRDVD---GGSAARFMSDLNNGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFT 360
           QLP DESKRD D     S +R   ++ +  G   G+ +GRN   +GRRFS PWPFNGLF+
Sbjct: 301 QLPADESKRDSDISRNSSNSRESEEIGDDVGGEEGDGDGRNG--NGRRFSIPWPFNGLFS 360

Query: 361 SQGNSSAGSASDSQRDHTS 362
             G+ S GS  +S    +S
Sbjct: 361 HSGSQSGGSVGNSSSSSSS 361

[Close]

BLAST of CSPI04G12450.1 vs. NCBI nr
Match: gi|694422761|ref|XP_009339205.1| (PREDICTED: E3 ubiquitin-protein ligase RING1-like [Pyrus x bretschneideri])

HSP 1 Score: 429.1 bits (1102), Expect = 7.7e-117
Identity = 236/375 (62.93%), Postives = 279/375 (74.40%), Query Frame = 1


Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60
           M++ IA RYWCHMCSQMVNPI+++E+KCPFC+SGF+EE+ NG + DN D           
Sbjct: 1   MEDAIAARYWCHMCSQMVNPIMEIEMKCPFCQSGFIEEM-NGTTRDNQD----------A 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFD-DDDDDNEHEDGEGN--RVSSETT 120
           +SDFGS+RALSLWAPILLGMM NPR RRR R L+FD DDDDDN ++DGE    R +    
Sbjct: 61  DSDFGSDRALSLWAPILLGMMNNPRRRRRLRRLDFDEDDDDDNNNDDGETRQGRDTELDR 120

Query: 121 ELDSVIRRRR-SSATILQLLQGIRAGIATAESENSYEG--------ERSRERERVILINP 180
           EL+S+IRRRR SSA+ LQLLQGIRAG+A+ ESENS EG        ER RERERVILINP
Sbjct: 121 ELESIIRRRRRSSASFLQLLQGIRAGLAS-ESENS-EGDRDRDRGRERERERERVILINP 180

Query: 181 FNQTIVVQGGENQNQ----NQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEA 240
           FNQTI+VQG  + NQ    N   I SLGDYF+GPGLDLLLQH+AENDPNRYGTPPAQKEA
Sbjct: 181 FNQTIIVQGSYDSNQGQINNHTPISSLGDYFIGPGLDLLLQHLAENDPNRYGTPPAQKEA 240

Query: 241 VDALPTVRVELEEDSCLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCR 300
           V+ALP V ++      +QCSVCLD+FEV  EAKEMPCKHKFH+GCILPWL+LHSSCPVCR
Sbjct: 241 VEALPAVTIKEN----MQCSVCLDDFEVGVEAKEMPCKHKFHSGCILPWLDLHSSCPVCR 300

Query: 301 HQLPGDESKRDVD---GGSAARFMSDLNN-GNGNGSGEIEGRNSSESGRRFSFPWPFNGL 356
            QLP DESKRD D     S +R   ++ + G   G G+ +GRN   +GRRFS PWPF+ L
Sbjct: 301 FQLPADESKRDSDISRTSSNSRESEEIGDVGGEEGDGDGDGRNG--NGRRFSIPWPFSSL 356

The following BLAST results are available for this feature:

BLAST of CSPI04G12450.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RNG1L_ARATH	2.2e-39	50.30	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana GN=At3g19950 PE=1...	[more]
RING1_GOSHI	4.2e-38	47.40	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum GN=RING1 PE=1 SV=1	[more]
RHC1A_ARATH	4.1e-25	40.36	Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana GN=RHC1A PE=2...	[more]
RN115_HUMAN	3.2e-22	43.80	E3 ubiquitin-protein ligase RNF115 OS=Homo sapiens GN=RNF115 PE=1 SV=2	[more]
RN115_MOUSE	9.4e-22	42.98	E3 ubiquitin-protein ligase RNF115 OS=Mus musculus GN=Rnf115 PE=1 SV=1	[more]

BLAST of CSPI04G12450.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KWR3_CUCSA	1.5e-207	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G267990 PE=4 SV=1	[more]
W9S1Q0_9ROSA	2.0e-124	66.12	E3 ubiquitin-protein ligase RING1 OS=Morus notabilis GN=L484_002344 PE=4 SV=1	[more]
M5VYQ4_PRUPE	1.3e-115	62.50	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007335mg PE=4 SV=1	[more]
A0A061GPC3_THECC	1.1e-114	60.16	RING/U-box superfamily protein isoform 1 OS=Theobroma cacao GN=TCM_038630 PE=4 S...	[more]
F6GUP5_VITVI	3.4e-111	59.57	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_06s0004g00120 PE=4 SV=...	[more]

BLAST of CSPI04G12450.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G56340.1	1.6e-104	54.34	RING/U-box superfamily protein	[more]
AT4G26400.2	1.2e-88	50.53	RING/U-box superfamily protein	[more]
AT1G55530.1	7.7e-83	48.53	RING/U-box superfamily protein	[more]
AT3G13430.1	5.0e-66	46.47	RING/U-box superfamily protein	[more]
AT3G19950.1	1.2e-40	50.30	RING/U-box superfamily protein	[more]

BLAST of CSPI04G12450.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449463838\|ref\|XP_004149638.1\|	2.1e-207	100.00	PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis sativus]	[more]
gi\|659097967\|ref\|XP_008449904.1\|	3.5e-202	97.81	PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo]	[more]
gi\|703133141\|ref\|XP_010105294.1\|	2.9e-124	66.12	E3 ubiquitin-protein ligase RING1 [Morus notabilis]	[more]
gi\|657951119\|ref\|XP_008351262.1\|	4.1e-118	62.01	PREDICTED: E3 ubiquitin-protein ligase CIP8-like [Malus domestica]	[more]
gi\|694422761\|ref\|XP_009339205.1\|	7.7e-117	62.93	PREDICTED: E3 ubiquitin-protein ligase RING1-like [Pyrus x bretschneideri]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR001841	Znf_RING
IPR013083	Znf_RING/FYVE/PHD

Vocabulary: **Molecular Function**
Term	Definition
GO:0005515	protein binding
GO:0008270	zinc ion binding

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005515	protein binding
molecular_function	GO:0008270	zinc ion binding

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI04G12450	CSPI04G12450	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI04G12450.1	CSPI04G12450.1-protein	polypeptide

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI04G12450.1.utr5p1	CSPI04G12450.1.utr5p1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI04G12450.1.cds1	CSPI04G12450.1.cds1	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI04G12450.1.utr3p1	CSPI04G12450.1.utr3p1	three_prime_UTR

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 242..284 score: 1.5
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 243..283 score: 7.
IPR001841	Zinc finger, RING-type	PROFILE	PS50089	ZF_RING_2	coord: 243..284 score: 12
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	G3DSA:3.30.40.10		coord: 238..287 score: 3.2
None	No IPR available	PANTHER	PTHR22763	RING ZINC FINGER PROTEIN	coord: 5..89 score: 3.4E-146coord: 105..357 score: 3.4E
None	No IPR available	PANTHER	PTHR22763:SF59	GENOMIC DNA, CHROMOSOME 3, P1 CLONE: MRP15	coord: 5..89 score: 3.4E-146coord: 105..357 score: 3.4E
None	No IPR available	PFAM	PF14369	zinc_ribbon_9	coord: 7..39 score: 7.2
None	No IPR available	unknown	SSF57850	RING/U-box	coord: 233..288 score: 2.87