Lsi10G003030 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi10G003030
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Probable carboxylesterase 2
Location	chr10 : 4844272 .. 4845195 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAACTGACGCCGCCGCCGGAGATGGGGTCGTTCACGAATTCCCCCCCGCCTTTTGCGTCTTAAAAAGCGGCCGCATCCAGAGGCTCTTCTCCGACGAACGCCTTCCACCGTCCACCGATCCACTAACCGGCGTCCAATCCAAAGACATCCTCATCGATCCACTAACCCAACTTTTCGTCCGAATCTACCTCCCAAATGTTTCAGACTCCACGAAGAAGCTCCCGCTTCTAATTTACATTCACGGCGGCGCTTTCTCCATCGGCTCCGTCACAGCTCCGATGTACCACTACCATCTGATCTCCCTCGCTGCCGAGGCCAACGCCATCGCTCTCTCGGTGGAATACCGTCTGGCGCCGGAGCACCCTCTCCCAGCGGCGTACGAGGACTCGTGGGCGGCGGTGGAATGGGCAGCATCGCATTCCAATCACGACGGCGCGGAGATTTGGCTGAACCAACACGCGGATTTCAACCGCGTATTCTTCGTTGGGGATAGCGCTGGGGGTAATATTTGCTACAATATGGTGGGCCGGGCCAGCGAGAGTGATAATGGGCTGAAGGGAATGAAGATAGTTGGGCTGGGCCTGGTTCATCCGTTTTTCATGATTGACCAGCCCGATAAGTTGATAGAGTATATTTTCCCCACAACAGTGTGTTTGGAGGATCCGAGGCTGAATCCATGGGTCAACCCGAGACTCGGAAAGCTACGATGTGAGCGAGTGTTGATAATGGCGGCAGAACTTGATGACCTAAAGGATAGGGCGCATGGGTTTCACGCAGCCTTAAGGGAGAGTGGGTTTGAAGGGTCGGTAGAGATAGTTGAGACTGAAGGGGAGATACACGTGTTTCATTTGTTTTACCCCGAGAAAGAGAAGGCTGCTGCATTGGTTAAAAAGGTCGCTTCCTTTATCCAATCAGTGTAA

mRNA sequence

ATGGAAACTGACGCCGCCGCCGGAGATGGGGTCGTTCACGAATTCCCCCCCGCCTTTTGCGTCTTAAAAAGCGGCCGCATCCAGAGGCTCTTCTCCGACGAACGCCTTCCACCGTCCACCGATCCACTAACCGGCGTCCAATCCAAAGACATCCTCATCGATCCACTAACCCAACTTTTCGTCCGAATCTACCTCCCAAATGTTTCAGACTCCACGAAGAAGCTCCCGCTTCTAATTTACATTCACGGCGGCGCTTTCTCCATCGGCTCCGTCACAGCTCCGATGTACCACTACCATCTGATCTCCCTCGCTGCCGAGGCCAACGCCATCGCTCTCTCGGTGGAATACCGTCTGGCGCCGGAGCACCCTCTCCCAGCGGCGTACGAGGACTCGTGGGCGGCGGTGGAATGGGCAGCATCGCATTCCAATCACGACGGCGCGGAGATTTGGCTGAACCAACACGCGGATTTCAACCGCGTATTCTTCGTTGGGGATAGCGCTGGGGGTAATATTTGCTACAATATGGTGGGCCGGGCCAGCGAGAGTGATAATGGGCTGAAGGGAATGAAGATAGTTGGGCTGGGCCTGGTTCATCCGTTTTTCATGATTGACCAGCCCGATAAGTTGATAGAGTATATTTTCCCCACAACAGTGTGTTTGGAGGATCCGAGGCTGAATCCATGGGTCAACCCGAGACTCGGAAAGCTACGATGTGAGCGAGTGTTGATAATGGCGGCAGAACTTGATGACCTAAAGGATAGGGCGCATGGGTTTCACGCAGCCTTAAGGGAGAGTGGGTTTGAAGGGTCGGTAGAGATAGTTGAGACTGAAGGGGAGATACACGTGTTTCATTTGTTTTACCCCGAGAAAGAGAAGGCTGCTGCATTGGTTAAAAAGGTCGCTTCCTTTATCCAATCAGTGTAA

Coding sequence (CDS)

ATGGAAACTGACGCCGCCGCCGGAGATGGGGTCGTTCACGAATTCCCCCCCGCCTTTTGCGTCTTAAAAAGCGGCCGCATCCAGAGGCTCTTCTCCGACGAACGCCTTCCACCGTCCACCGATCCACTAACCGGCGTCCAATCCAAAGACATCCTCATCGATCCACTAACCCAACTTTTCGTCCGAATCTACCTCCCAAATGTTTCAGACTCCACGAAGAAGCTCCCGCTTCTAATTTACATTCACGGCGGCGCTTTCTCCATCGGCTCCGTCACAGCTCCGATGTACCACTACCATCTGATCTCCCTCGCTGCCGAGGCCAACGCCATCGCTCTCTCGGTGGAATACCGTCTGGCGCCGGAGCACCCTCTCCCAGCGGCGTACGAGGACTCGTGGGCGGCGGTGGAATGGGCAGCATCGCATTCCAATCACGACGGCGCGGAGATTTGGCTGAACCAACACGCGGATTTCAACCGCGTATTCTTCGTTGGGGATAGCGCTGGGGGTAATATTTGCTACAATATGGTGGGCCGGGCCAGCGAGAGTGATAATGGGCTGAAGGGAATGAAGATAGTTGGGCTGGGCCTGGTTCATCCGTTTTTCATGATTGACCAGCCCGATAAGTTGATAGAGTATATTTTCCCCACAACAGTGTGTTTGGAGGATCCGAGGCTGAATCCATGGGTCAACCCGAGACTCGGAAAGCTACGATGTGAGCGAGTGTTGATAATGGCGGCAGAACTTGATGACCTAAAGGATAGGGCGCATGGGTTTCACGCAGCCTTAAGGGAGAGTGGGTTTGAAGGGTCGGTAGAGATAGTTGAGACTGAAGGGGAGATACACGTGTTTCATTTGTTTTACCCCGAGAAAGAGAAGGCTGCTGCATTGGTTAAAAAGGTCGCTTCCTTTATCCAATCAGTGTAA

Protein sequence

METDAAAGDGVVHEFPPAFCVLKSGRIQRLFSDERLPPSTDPLTGVQSKDILIDPLTQLFVRIYLPNVSDSTKKLPLLIYIHGGAFSIGSVTAPMYHYHLISLAAEANAIALSVEYRLAPEHPLPAAYEDSWAAVEWAASHSNHDGAEIWLNQHADFNRVFFVGDSAGGNICYNMVGRASESDNGLKGMKIVGLGLVHPFFMIDQPDKLIEYIFPTTVCLEDPRLNPWVNPRLGKLRCERVLIMAAELDDLKDRAHGFHAALRESGFEGSVEIVETEGEIHVFHLFYPEKEKAAALVKKVASFIQSV

Homology

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BLAST of Lsi10G003030 vs. Swiss-Prot
Match: CXE2_ARATH (Probable carboxylesterase 2 OS=Arabidopsis thaliana GN=CXE2 PE=2 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 3.5e-62
Identity = 133/310 (42.90%), Postives = 186/310 (60.00%), Query Frame = 1


Query: 14  EFPPAFCVLKSGRIQRLFSDERLPPSTDPLTGVQSKDILIDPLTQLFVRIYLPNVSDSTK 73
           E  P   V   G ++RL   E  PP  DP+TGV SKDI+I+P T L  RIY P      +
Sbjct: 11  ELLPWLVVHTDGTVERLAGTEVCPPGLDPITGVFSKDIIIEPKTGLSARIYRPFSIQPGQ 70

Query: 74  KLPLLIYIHGGAFSIGSVTAPMYHYHLISLAAEANAIALSVEYRLAPEHPLPAAYEDSWA 133
           K+PL++Y HGGAF I S + P YH  L  +  +AN IA+SV YRLAPEHPLP AYEDSW 
Sbjct: 71  KIPLMLYFHGGAFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLAPEHPLPTAYEDSWT 130

Query: 134 AVEWAASHSNHDGAEIWLNQHADFNRVFFVGDSAGGNICYNMVGRASESDNGLKGMKIVG 193
           A++   + +     E W+N +AD + +F VGDSAG NI +++  RA +SD  L   KI G
Sbjct: 131 ALKNIQAIN-----EPWINDYADLDSLFLVGDSAGANISHHLAFRAKQSDQTL---KIKG 190

Query: 194 LGLVHPFFMIDQP--------------DKLIEYIFPTTVCLEDPRLNPWV--NPRLGKLR 253
           +G++HP+F   QP              D   E++ P+    +DP +NP+   +P LG L 
Sbjct: 191 IGMIHPYFWGTQPIGAEIKDEARKQMVDGWWEFVCPSEKGSDDPWINPFADGSPDLGGLG 250

Query: 254 CERVLIMAAELDDLKDRAHGFHAALRESGFEGSVEIVETEGEIHVFHLFYPEKEKAAALV 308
           CERV+I  AE D L +R   ++  L +S ++G VEI+ET+ + HVFH+F P+ ++A  +V
Sbjct: 251 CERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFHIFEPDCDEAMEMV 310

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BLAST of Lsi10G003030 vs. Swiss-Prot
Match: CXE12_ARATH (Probable carboxylesterase 12 OS=Arabidopsis thaliana GN=CXE12 PE=1 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 3.6e-59
Identity = 128/311 (41.16%), Postives = 181/311 (58.20%), Query Frame = 1


Query: 17  PAFCVLKSGRIQRLFSDERLPPSTDPLTGVQSKDILIDPLTQLFVRIYLPN--VSDSTKK 76
           P   + KSGRI+RL  +  +PPS++P  GV SKD++      L VRIYLP    +++  K
Sbjct: 11  PLLKIYKSGRIERLMGEATVPPSSEPQNGVVSKDVVYSADNNLSVRIYLPEKAAAETDSK 70

Query: 77  LPLLIYIHGGAFSIGSVTAPMYHYHLISLAAEANAIALSVEYRLAPEHPLPAAYEDSWAA 136
           LPLL+Y HGG F I +  +P YH  L +  + +N +A+SV+YR APEHP+   ++DSW A
Sbjct: 71  LPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPEHPISVPFDDSWTA 130

Query: 137 VEWAASHSNHDGAEIWLNQHADFNRVFFVGDSAGGNICYNMVGRASES--DNGLKGMKIV 196
           ++W  +H    G E WLN+HADF+RVF  GDSAG NI ++M  RA++     GL    I 
Sbjct: 131 LKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKEKLSPGLNDTGIS 190

Query: 197 GLGLVHPFFM----IDQPDKLIEYIFPTTVCL------------EDPRLNPWVNPR--LG 256
           G+ L+HP+F     ID+ D   E +                   +DP LN   +    L 
Sbjct: 191 GIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDPLLNVVQSESVDLS 250

Query: 257 KLRCERVLIMAAELDDLKDRAHGFHAALRESGFEGSVEIVETEGEIHVFHLFYPEKEKAA 306
            L C +VL+M AE D L  +  G+ A L +SG++G VE+VE+EGE HVFHL  PE + A 
Sbjct: 251 GLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDHVFHLLKPECDNAI 310

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BLAST of Lsi10G003030 vs. Swiss-Prot
Match: CXE13_ARATH (Probable carboxylesterase 13 OS=Arabidopsis thaliana GN=CXE13 PE=1 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 6.8e-58
Identity = 127/322 (39.44%), Postives = 185/322 (57.45%), Query Frame = 1


Query: 11  VVHEFPPAFCVLKSGRIQRLFSDERLPPSTDPLTGVQSKDILIDPLTQLFVRIYLPNV-- 70
           +  ++ P   + KSGRI+RL  +  +PPS++P  GV SKD++  P   L +RIYLP    
Sbjct: 5   IAADYSPMLIIYKSGRIERLVGETTVPPSSNPQNGVVSKDVVYSPDNNLSLRIYLPEKAA 64

Query: 71  ---SDSTKKLPLLIYIHGGAFSIGSVTAPMYHYHLISLAAEANAIALSVEYRLAPEHPLP 130
              ++++ KLPLL+Y HGG F + +  +P YH  L +  + ++ +A+SV+YR APEHP+P
Sbjct: 65  TAETEASVKLPLLVYFHGGGFLVETAFSPTYHTFLTAAVSASDCVAVSVDYRRAPEHPIP 124

Query: 131 AAYEDSWAAVEWAASHSNHDGAEIWLNQHADFNRVFFVGDSAGGNICYNMVGRASE---S 190
            +Y+DSW A++W  SH    G+E WLN+HADF++VF  GDSAG NI ++M  +A++   S
Sbjct: 125 TSYDDSWTALKWVFSHIAGSGSEDWLNKHADFSKVFLAGDSAGANITHHMTMKAAKDKLS 184

Query: 191 DNGLKGMKIVGLGLVHPFFMIDQP-------DKLIEYIFPTTVCLEDPRL-----NPWVN 250
              L    I G+ LVHP+F    P       D  I     +   L  P       +P++N
Sbjct: 185 PESLNESGISGIILVHPYFWSKTPVDDKETTDVAIRTWIESVWTLASPNSKDGSDDPFIN 244

Query: 251 ------PRLGKLRCERVLIMAAELDDLKDRAHGFHAALRESGFEGSV-EIVETEGEIHVF 306
                   L  L C +VL+M AE D L  +  G+   L +S + G V ++VET+GE HVF
Sbjct: 245 VVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYWEKLGKSRWNGEVLDVVETKGEGHVF 304

[Close]

BLAST of Lsi10G003030 vs. Swiss-Prot
Match: HIDM_GLYEC (2-hydroxyisoflavanone dehydratase OS=Glycyrrhiza echinata GN=HIDM PE=1 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 6.8e-58
Identity = 120/325 (36.92%), Postives = 190/325 (58.46%), Query Frame = 1


Query: 3   TDAAAGDGVVHEFPPAFCVLKSGRIQRLFSDERLPPST-DPLTGVQSKDILIDPLTQLFV 62
           T       +  E PP   V K G ++R      +PPS  DP TGV +KDI+I     +  
Sbjct: 5   TSTTTSKEIDRELPPLLRVYKDGTVERFLGSSFVPPSPEDPETGVSTKDIVISENPTISA 64

Query: 63  RIYLPNVSDSTKKLPLLIYIHGGAFSIGSVTAPMYHYHLISLAAEANAIALSVEYRLAPE 122
           R+YLP ++++T+KLP+L+Y HGGAF + S  + ++  +L  +A++AN + +S+EYRLAPE
Sbjct: 65  RVYLPKLNNTTEKLPILVYYHGGAFCLESAFSFLHQRYLNIVASKANVLVVSIEYRLAPE 124

Query: 123 HPLPAAYEDSWAAVEWAASHSNHD----GAEIWLNQHADFNRVFFVGDSAGGNICYNMVG 182
           HPLPAAYED W A++W  SHS ++     A+ WL +H DFNR +  GD++G NI +N   
Sbjct: 125 HPLPAAYEDGWYALKWVTSHSTNNNKPTNADPWLIKHGDFNRFYIGGDTSGANIAHNAAL 184

Query: 183 R-ASESDNGLKGMKIVGLGLVHPFFM--------------IDQPDKLIEYIFPTTV-CLE 242
           R  +E+  G  G++I G+    P F                  P ++  +++P     ++
Sbjct: 185 RVGAEALPG--GLRIAGVLSAFPLFWGSKPVLSEPVEGHEKSSPMQVWNFVYPDAPGGID 244

Query: 243 DPRLNPWV--NPRLGKLRCERVLIMAAELDDLKDRAHGFHAALRESGFEGSVEIVETEGE 302
           +P +NP     P L  L C ++L+  A  DDL+DR   ++ A++ESG++G VE+ + EGE
Sbjct: 245 NPLINPLAPGAPNLATLGCPKMLVFVAGKDDLRDRGIWYYEAVKESGWKGDVELAQYEGE 304

Query: 303 IHVFHLFYPEKEKAAALVKKVASFI 305
            H F +++PE E +  L+ ++ASF+
Sbjct: 305 EHCFQIYHPETENSKDLIGRIASFL 327

[Close]

BLAST of Lsi10G003030 vs. Swiss-Prot
Match: CXE1_ARATH (Probable carboxylesterase 1 OS=Arabidopsis thaliana GN=CXE1 PE=2 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 7.6e-57
Identity = 121/310 (39.03%), Postives = 176/310 (56.77%), Query Frame = 1


Query: 11  VVHEFPPAFCVLKSGRIQRLFSDERLPPSTDPLTGVQSKDILIDPLTQLFVRIYLPNVS- 70
           +  ++ P F + K+G I+RL  +  +PPS +P  GV SKD +  P   L +RIYLP  S 
Sbjct: 5   IAFDYSPRFRIFKNGGIERLVPETFVPPSLNPENGVVSKDAVYSPEKNLSLRIYLPQNSV 64

Query: 71  --DSTKKLPLLIYIHGGAFSIGSVTAPMYHYHLISLAAEANAIALSVEYRLAPEHPLPAA 130
                KK+PLL+Y HGG F + +  +P+YH  L S  +  + IA+SVEYR APEHP+P  
Sbjct: 65  YETGEKKIPLLVYFHGGGFIMETAFSPIYHTFLTSAVSATDCIAVSVEYRRAPEHPIPTL 124

Query: 131 YEDSWAAVEWAASHSNHDGAEIWLNQHADFNRVFFVGDSAGGNICYNMVGRASESDNGLK 190
           YEDSW A++W  +H    G E WLN+HADF++VF  GDSAG NI ++M  R  +     +
Sbjct: 125 YEDSWDAIQWIFTHITRSGPEDWLNKHADFSKVFLAGDSAGANIAHHMAIRVDKEKLPPE 184

Query: 191 GMKIVGLGLVHPFF----MIDQPDKLIEYIFPTTVCLEDPRL-----NPWVN---PRLGK 250
             KI G+ L HP+F    +I++ +      +     +  P       +PW+N     L  
Sbjct: 185 NFKISGMILFHPYFLSKALIEEMEVEAMRYYERLWRIASPDSGNGVEDPWINVVGSDLTG 244

Query: 251 LRCERVLIMAAELDDLKDRAHGFHAALRESGFEGSVEIVETEGEIHVFHLFYPEKEKAAA 306
           L C RVL+M A  D L      + A L +SG+ G V+++ET+ E HVFHL  P+ E A  
Sbjct: 245 LGCRRVLVMVAGNDVLARGGWSYVAELEKSGWIGKVKVMETKEEGHVFHLRDPDSENARR 304

[Close]

BLAST of Lsi10G003030 vs. TrEMBL
Match: Q0ZPW5_MALPM (CXE carboxylesterase OS=Malus pumila GN=CXE5 PE=2 SV=1)

HSP 1 Score: 331.3 bits (848), Expect = 1.3e-87
Identity = 162/301 (53.82%), Postives = 212/301 (70.43%), Query Frame = 1


Query: 4   DAAAGDGVVHEFPPAFCVLKSGRIQRLFSDERLPPSTDPLTGVQSKDILIDPLTQLFVRI 63
           D+     V  EFP AF +   GR +R    E +PPSTD  TGVQ KDI++ P + L  R+
Sbjct: 2   DSKPSSEVSFEFPTAFRIYNDGRTERFKGIETVPPSTDSTTGVQCKDIVLSPQSGLSARV 61

Query: 64  YLPNVSDSTKKLPLLIYIHGGAFSIGSVTAPMYHYHLISLAAEANAIALSVEYRLAPEHP 123
           +LP + D T+KLPLLI+IHGGAF I S  +P+YH H++ LA+EAN +ALSV YR APEHP
Sbjct: 62  FLPKLPDPTRKLPLLIFIHGGAFVIESPYSPLYHKHVMLLASEANVVALSVHYRRAPEHP 121

Query: 124 LPAAYEDSWAAVEWAASHSNHDGAEIWLNQHADFNRVFFVGDSAGGNICYNMVGRASESD 183
           LP A+EDSW AVEWAA+HS  +G E WLN H DF+RVF  GDSAG  + +++V +A    
Sbjct: 122 LPVAFEDSWDAVEWAAAHSTRNGPEAWLNDHVDFDRVFIGGDSAGATLTHHVVRQAGL-- 181

Query: 184 NGLKGMKIVGLGLVHPFFMIDQPDKLIEYIFPTTVCLEDPRLNPWVNPRLGKLRCERVLI 243
           +GL G +IVG+ L HP+FM D+PDKL+E I+PT    +DPR+ P  +P+LG++ C RVL+
Sbjct: 182 DGLSGTRIVGMILFHPYFMDDEPDKLLEVIYPTCGGSDDPRVRPGNDPKLGEIGCGRVLV 241

Query: 244 MAAELDDLKDRAHGFHAALRESGFEGSVEIVETEGEIHVFHLFYPEKEKAAALVKKVASF 303
             AE D L+DR   +H AL++SG+ G VEIVE++GE HVFHLF P  + A  LVKKV SF
Sbjct: 242 FVAEKDFLRDRGWAYHEALKKSGYGGVVEIVESQGEDHVFHLFNPSCDNAVDLVKKVVSF 300

Query: 304 I 305
           +
Sbjct: 302 V 300

[Close]

BLAST of Lsi10G003030 vs. TrEMBL
Match: M5VQ13_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa009160mg PE=4 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 2.4e-86
Identity = 161/291 (55.33%), Postives = 211/291 (72.51%), Query Frame = 1


Query: 14  EFPPAFCVLKSGRIQRLFSDERLPPSTDPLTGVQSKDILIDPLTQLFVRIYLPNVSDSTK 73
           EFP  F + K GR++RL   E +PPSTDP TGV+SKDI++   + L  RI+LP ++D T+
Sbjct: 13  EFPTIFRIYKDGRVERLKGTETVPPSTDPKTGVRSKDIVLSHESGLSARIFLPKLTDPTR 72

Query: 74  KLPLLIYIHGGAFSIGSVTAPMYHYHLISLAAEANAIALSVEYRLAPEHPLPAAYEDSWA 133
           KLPLL++IHGGAF I S  +P+YH H   L +EAN + LSV YR APEHPLP AYEDSW 
Sbjct: 73  KLPLLVFIHGGAFVIESPYSPLYHNHAALLTSEANVVVLSVNYRRAPEHPLPVAYEDSWE 132

Query: 134 AVEWAASHSNHDGAEIWLNQHADFNRVFFVGDSAGGNICYNMVGRASESDNGLKGMKIVG 193
           A++WAA+HSN +G E WLN + +F+RVF  GDSAG  + +++V RA    +GL G KIVG
Sbjct: 133 ALQWAAAHSNRNGPEAWLNDYVNFDRVFVGGDSAGATLTHHVVHRAGV--DGLSGAKIVG 192

Query: 194 LGLVHPFFMIDQPDKLIEYIFPTTVCLEDPRLNPWVNPRLGKLRCERVLIMAAELDDLKD 253
           + L HPFF   + DKL+E IFP    L+DPR+NP  +P+LGK+ C RVL+  AE D L+D
Sbjct: 193 MILFHPFFGNGKLDKLLEVIFPECNGLDDPRVNPGKDPKLGKIGCRRVLVFVAEKDFLRD 252

Query: 254 RAHGFHAALRESGFEGSVEIVETEGEIHVFHLFYPEKEKAAALVKKVASFI 305
           RA  ++ +L++SG+ G VEI+E+EGE HVFHLF P  +KA ALV+KV SFI
Sbjct: 253 RAWAYYESLKKSGWGGVVEILESEGEDHVFHLFNPSCDKAVALVQKVVSFI 301

[Close]

BLAST of Lsi10G003030 vs. TrEMBL
Match: B9HRY0_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0009s10800g PE=4 SV=2)

HSP 1 Score: 320.1 bits (819), Expect = 3.0e-84
Identity = 163/310 (52.58%), Postives = 214/310 (69.03%), Query Frame = 1


Query: 4   DAAAGDGVVHEFPPAFCVLKSGRIQRLFSD-ERLPPSTDPLTGVQSKDILIDPLTQLFVR 63
           D++   G++H+FPP F V ++G+++R+ +D E + PS DP TGVQSKD ++     L VR
Sbjct: 2   DSSNSTGILHDFPPFFRVYRNGKVERITADAETVRPSNDPHTGVQSKDTVVSQENSLSVR 61

Query: 64  IYLPNVSDSTKKLPLLIYIHGGAFSIGSVTAPMYHYHLISLAAEANAIALSVEYRLAPEH 123
           +++P + D ++KLPLLIYIHGGAF I S  + MYH +L +LA +AN IA+SV+YR APEH
Sbjct: 62  LFIPKIKDPSQKLPLLIYIHGGAFCIESPFSSMYHNYLTNLAHQANVIAVSVQYRRAPEH 121

Query: 124 PLPAAYEDSWAAVEWAASHSNHDGAEIWLNQHADFNRVFFVGDSAGGNICYNMVGRASES 183
           PLP AY+DSWAA++W ASH N  G E WLN+HADF R F  GDSAG NI +NM  RA   
Sbjct: 122 PLPIAYDDSWAAIQWVASHVNGIGVESWLNKHADFERTFLAGDSAGANIAHNMTVRAGV- 181

Query: 184 DNGLKGMKIVGLGLVHPFFMIDQPD---KLIEYIFPTTVCLEDPRLNP--WVNPRLGKLR 243
            NGL G+K VG+ L HPFF   +PD    +IEYIFP     +DPR+NP       L  L 
Sbjct: 182 -NGLFGVKTVGMVLAHPFFGGKEPDFFSPVIEYIFPDVKIYDDPRINPAGAGGVELASLG 241

Query: 244 CERVLIMAAELDDLKDRAHGFHAALRESGFEGSVEIVETEGEIHVFHLFYPEKEKAAALV 303
           C RVLI  A  D L++R + ++ AL++SG+ G VEIVETEGE HVFHLF P+ +KA  ++
Sbjct: 242 CSRVLIFVAGNDGLRERGYSYYDALKKSGWSGVVEIVETEGEDHVFHLFNPDCDKAVFMM 301

Query: 304 KKVASFIQSV 308
           K+V SFI  V
Sbjct: 302 KQVVSFINPV 309

[Close]

BLAST of Lsi10G003030 vs. TrEMBL
Match: A0A061E1M8_THECC (Alpha/beta-Hydrolases superfamily protein, putative OS=Theobroma cacao GN=TCM_007027 PE=4 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 2.1e-82
Identity = 153/289 (52.94%), Postives = 208/289 (71.97%), Query Frame = 1


Query: 17  PAFCVLKSGRIQRLFSDERLPPSTDPLTGVQSKDILIDPLTQLFVRIYLPNVSDSTKKLP 76
           P F V K GRI+RL   E +PPS DP TGVQSKD ++ P + L  R++LP ++D T K+P
Sbjct: 17  PLFRVYKDGRIERLRETETVPPSNDPQTGVQSKDTIVLPESSLSARLFLPKITDPTAKIP 76

Query: 77  LLIYIHGGAFSIGSVTAPMYHYHLISLAAEANAIALSVEYRLAPEHPLPAAYEDSWAAVE 136
           L+IYIHGGAF I S  +P+YH +L SL  +AN IA++++YR APE+PLP AY+D+W A++
Sbjct: 77  LVIYIHGGAFCIESPFSPLYHNYLTSLVNKANVIAVAIQYRKAPEYPLPIAYDDAWTAIK 136

Query: 137 WAASHSNHDGAEIWLNQHADFNRVFFVGDSAGGNICYNMVGRASESDNGLKGMKIVGLGL 196
           W ASH+N DG E WLN HADF RVFF GDSAG NI +NM  +A    +GL G+K+VG+ L
Sbjct: 137 WVASHANRDGPEPWLNDHADFERVFFAGDSAGANIAHNMTMKAGA--DGLIGVKLVGMLL 196

Query: 197 VHPFFMIDQPDKLIEYIFPTTVCLEDPRLNP-WVNPRLGKLRCERVLIMAAELDDLKDRA 256
           ++PFF  ++PD+LIEYIFP++    DPR+NP      L  L C RVL+  +E D LKDR 
Sbjct: 197 MNPFFRNNEPDELIEYIFPSSSGCNDPRMNPASAIKELAGLACSRVLVCVSEKDFLKDRG 256

Query: 257 HGFHAALRESGFEGSVEIVETEGEIHVFHLFYPEKEKAAALVKKVASFI 305
             ++ A+R+SG++G ++IVET GE HVF+LF P  +KA  L+ +V+SF+
Sbjct: 257 VTYYEAVRKSGWDGVIDIVETPGEKHVFYLFDPSSQKAMDLMDQVSSFL 303

[Close]

BLAST of Lsi10G003030 vs. TrEMBL
Match: W9SMM8_9ROSA (Putative carboxylesterase 7 OS=Morus notabilis GN=L484_027556 PE=4 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 6.2e-82
Identity = 163/297 (54.88%), Postives = 207/297 (69.70%), Query Frame = 1


Query: 9   DGVVHEFPPAFCVLKSGRIQRLFSDERLPPSTDPLTGVQSKDILIDPLTQLFVRIYLPNV 68
           D V  EFP  F + K GRI R F DE +PPS D  T  +SKD++I P + L  R+++  +
Sbjct: 6   DEVAIEFP-FFRIYKDGRIDRFFGDEIVPPSPDSATAARSKDVVISPESGLSARLFIHQI 65

Query: 69  SDSTKKLPLLIYIHGGAFSIGSVTAPMYHYHLISLAAEANAIALSVEYRLAPEHPLPAAY 128
            D T+KLPLL+Y+HGGAF +G+  +P Y  H  SLAAE+NAI LSV YR APEHPLP AY
Sbjct: 66  PDPTRKLPLLVYLHGGAFCVGTPFSPTYTGHASSLAAESNAIVLSVHYRRAPEHPLPIAY 125

Query: 129 EDSWAAVEWAASHSNHDGA-EIWLNQHADFNRVFFVGDSAGGNICYNMVGRASESDNGLK 188
           +D+W A++WA  HS   G  E WLNQHADF+RVF  GDSAG NI + +V RA    +GL 
Sbjct: 126 DDAWEALQWAVVHSGGGGGPEPWLNQHADFDRVFVGGDSAGANIAHYVVLRAGV--DGLN 185

Query: 189 GMKIVGLGLVHPFFMIDQPDKLIEYIFPTTVCLEDPRLNPWVNPRLGKLRCERVLIMAAE 248
           G +I GL LVHPFF  D+P++L+E IFP+     DPR+NP  +P L +L C RVLI  AE
Sbjct: 186 GPRIAGLVLVHPFFGNDKPNRLLEIIFPSRSVPNDPRVNPGFDPDLSRLGCGRVLIFVAE 245

Query: 249 LDDLKDRAHGFHAALRESGFEGSVEIVETEGEIHVFHLFYPEKEKAAALVKKVASFI 305
            D LKDRA  ++ AL++SG+ G VE+VE EG  HVFHLF P+ E+A ALVKKV+SF+
Sbjct: 246 KDFLKDRALAYYEALKKSGWIGLVELVEYEGVDHVFHLFSPKCEQAQALVKKVSSFL 299

[Close]

BLAST of Lsi10G003030 vs. TAIR10
Match: AT1G47480.1 (AT1G47480.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 240.0 bits (611), Expect = 2.0e-63
Identity = 133/310 (42.90%), Postives = 186/310 (60.00%), Query Frame = 1


Query: 14  EFPPAFCVLKSGRIQRLFSDERLPPSTDPLTGVQSKDILIDPLTQLFVRIYLPNVSDSTK 73
           E  P   V   G ++RL   E  PP  DP+TGV SKDI+I+P T L  RIY P      +
Sbjct: 11  ELLPWLVVHTDGTVERLAGTEVCPPGLDPITGVFSKDIIIEPKTGLSARIYRPFSIQPGQ 70

Query: 74  KLPLLIYIHGGAFSIGSVTAPMYHYHLISLAAEANAIALSVEYRLAPEHPLPAAYEDSWA 133
           K+PL++Y HGGAF I S + P YH  L  +  +AN IA+SV YRLAPEHPLP AYEDSW 
Sbjct: 71  KIPLMLYFHGGAFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLAPEHPLPTAYEDSWT 130

Query: 134 AVEWAASHSNHDGAEIWLNQHADFNRVFFVGDSAGGNICYNMVGRASESDNGLKGMKIVG 193
           A++   + +     E W+N +AD + +F VGDSAG NI +++  RA +SD  L   KI G
Sbjct: 131 ALKNIQAIN-----EPWINDYADLDSLFLVGDSAGANISHHLAFRAKQSDQTL---KIKG 190

Query: 194 LGLVHPFFMIDQP--------------DKLIEYIFPTTVCLEDPRLNPWV--NPRLGKLR 253
           +G++HP+F   QP              D   E++ P+    +DP +NP+   +P LG L 
Sbjct: 191 IGMIHPYFWGTQPIGAEIKDEARKQMVDGWWEFVCPSEKGSDDPWINPFADGSPDLGGLG 250

Query: 254 CERVLIMAAELDDLKDRAHGFHAALRESGFEGSVEIVETEGEIHVFHLFYPEKEKAAALV 308
           CERV+I  AE D L +R   ++  L +S ++G VEI+ET+ + HVFH+F P+ ++A  +V
Sbjct: 251 CERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFHIFEPDCDEAMEMV 310

[Close]

BLAST of Lsi10G003030 vs. TAIR10
Match: AT3G48690.1 (AT3G48690.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 229.9 bits (585), Expect = 2.0e-60
Identity = 128/311 (41.16%), Postives = 181/311 (58.20%), Query Frame = 1


Query: 17  PAFCVLKSGRIQRLFSDERLPPSTDPLTGVQSKDILIDPLTQLFVRIYLPN--VSDSTKK 76
           P   + KSGRI+RL  +  +PPS++P  GV SKD++      L VRIYLP    +++  K
Sbjct: 11  PLLKIYKSGRIERLMGEATVPPSSEPQNGVVSKDVVYSADNNLSVRIYLPEKAAAETDSK 70

Query: 77  LPLLIYIHGGAFSIGSVTAPMYHYHLISLAAEANAIALSVEYRLAPEHPLPAAYEDSWAA 136
           LPLL+Y HGG F I +  +P YH  L +  + +N +A+SV+YR APEHP+   ++DSW A
Sbjct: 71  LPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPEHPISVPFDDSWTA 130

Query: 137 VEWAASHSNHDGAEIWLNQHADFNRVFFVGDSAGGNICYNMVGRASES--DNGLKGMKIV 196
           ++W  +H    G E WLN+HADF+RVF  GDSAG NI ++M  RA++     GL    I 
Sbjct: 131 LKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKEKLSPGLNDTGIS 190

Query: 197 GLGLVHPFFM----IDQPDKLIEYIFPTTVCL------------EDPRLNPWVNPR--LG 256
           G+ L+HP+F     ID+ D   E +                   +DP LN   +    L 
Sbjct: 191 GIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDPLLNVVQSESVDLS 250

Query: 257 KLRCERVLIMAAELDDLKDRAHGFHAALRESGFEGSVEIVETEGEIHVFHLFYPEKEKAA 306
            L C +VL+M AE D L  +  G+ A L +SG++G VE+VE+EGE HVFHL  PE + A 
Sbjct: 251 GLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDHVFHLLKPECDNAI 310

[Close]

BLAST of Lsi10G003030 vs. TAIR10
Match: AT3G48700.1 (AT3G48700.1 carboxyesterase 13)

HSP 1 Score: 225.7 bits (574), Expect = 3.9e-59
Identity = 127/322 (39.44%), Postives = 185/322 (57.45%), Query Frame = 1


Query: 11  VVHEFPPAFCVLKSGRIQRLFSDERLPPSTDPLTGVQSKDILIDPLTQLFVRIYLPNV-- 70
           +  ++ P   + KSGRI+RL  +  +PPS++P  GV SKD++  P   L +RIYLP    
Sbjct: 5   IAADYSPMLIIYKSGRIERLVGETTVPPSSNPQNGVVSKDVVYSPDNNLSLRIYLPEKAA 64

Query: 71  ---SDSTKKLPLLIYIHGGAFSIGSVTAPMYHYHLISLAAEANAIALSVEYRLAPEHPLP 130
              ++++ KLPLL+Y HGG F + +  +P YH  L +  + ++ +A+SV+YR APEHP+P
Sbjct: 65  TAETEASVKLPLLVYFHGGGFLVETAFSPTYHTFLTAAVSASDCVAVSVDYRRAPEHPIP 124

Query: 131 AAYEDSWAAVEWAASHSNHDGAEIWLNQHADFNRVFFVGDSAGGNICYNMVGRASE---S 190
            +Y+DSW A++W  SH    G+E WLN+HADF++VF  GDSAG NI ++M  +A++   S
Sbjct: 125 TSYDDSWTALKWVFSHIAGSGSEDWLNKHADFSKVFLAGDSAGANITHHMTMKAAKDKLS 184

Query: 191 DNGLKGMKIVGLGLVHPFFMIDQP-------DKLIEYIFPTTVCLEDPRL-----NPWVN 250
              L    I G+ LVHP+F    P       D  I     +   L  P       +P++N
Sbjct: 185 PESLNESGISGIILVHPYFWSKTPVDDKETTDVAIRTWIESVWTLASPNSKDGSDDPFIN 244

Query: 251 ------PRLGKLRCERVLIMAAELDDLKDRAHGFHAALRESGFEGSV-EIVETEGEIHVF 306
                   L  L C +VL+M AE D L  +  G+   L +S + G V ++VET+GE HVF
Sbjct: 245 VVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYWEKLGKSRWNGEVLDVVETKGEGHVF 304

[Close]

BLAST of Lsi10G003030 vs. TAIR10
Match: AT1G19190.1 (AT1G19190.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 222.2 bits (565), Expect = 4.3e-58
Identity = 121/310 (39.03%), Postives = 176/310 (56.77%), Query Frame = 1


Query: 11  VVHEFPPAFCVLKSGRIQRLFSDERLPPSTDPLTGVQSKDILIDPLTQLFVRIYLPNVS- 70
           +  ++ P F + K+G I+RL  +  +PPS +P  GV SKD +  P   L +RIYLP  S 
Sbjct: 5   IAFDYSPRFRIFKNGGIERLVPETFVPPSLNPENGVVSKDAVYSPEKNLSLRIYLPQNSV 64

Query: 71  --DSTKKLPLLIYIHGGAFSIGSVTAPMYHYHLISLAAEANAIALSVEYRLAPEHPLPAA 130
                KK+PLL+Y HGG F + +  +P+YH  L S  +  + IA+SVEYR APEHP+P  
Sbjct: 65  YETGEKKIPLLVYFHGGGFIMETAFSPIYHTFLTSAVSATDCIAVSVEYRRAPEHPIPTL 124

Query: 131 YEDSWAAVEWAASHSNHDGAEIWLNQHADFNRVFFVGDSAGGNICYNMVGRASESDNGLK 190
           YEDSW A++W  +H    G E WLN+HADF++VF  GDSAG NI ++M  R  +     +
Sbjct: 125 YEDSWDAIQWIFTHITRSGPEDWLNKHADFSKVFLAGDSAGANIAHHMAIRVDKEKLPPE 184

Query: 191 GMKIVGLGLVHPFF----MIDQPDKLIEYIFPTTVCLEDPRL-----NPWVN---PRLGK 250
             KI G+ L HP+F    +I++ +      +     +  P       +PW+N     L  
Sbjct: 185 NFKISGMILFHPYFLSKALIEEMEVEAMRYYERLWRIASPDSGNGVEDPWINVVGSDLTG 244

Query: 251 LRCERVLIMAAELDDLKDRAHGFHAALRESGFEGSVEIVETEGEIHVFHLFYPEKEKAAA 306
           L C RVL+M A  D L      + A L +SG+ G V+++ET+ E HVFHL  P+ E A  
Sbjct: 245 LGCRRVLVMVAGNDVLARGGWSYVAELEKSGWIGKVKVMETKEEGHVFHLRDPDSENARR 304

[Close]

BLAST of Lsi10G003030 vs. TAIR10
Match: AT1G49660.1 (AT1G49660.1 carboxyesterase 5)

HSP 1 Score: 220.7 bits (561), Expect = 1.2e-57
Identity = 130/317 (41.01%), Postives = 182/317 (57.41%), Query Frame = 1


Query: 11  VVHEFPPAFCVLKSGRIQRLFSDERLPPSTDPLTGVQSKDILIDPLTQLFVRIYLPNVSD 70
           +  EF P   + K GR++RL   + +P S DP   V SKD++  P   L VR++LP+ S 
Sbjct: 5   IASEFLPFCRIYKDGRVERLIGTDTIPASLDPTYDVVSKDVIYSPENNLSVRLFLPHKST 64

Query: 71  ST---KKLPLLIYIHGGAFSIGSVTAPMYHYHLISLAAEANAIALSVEYRLAPEHPLPAA 130
                 KLPLLIYIHGGA+ I S  +P+YH +L  +   AN +A+SV+YR APE P+PAA
Sbjct: 65  KLTAGNKLPLLIYIHGGAWIIESPFSPLYHNYLTEVVKSANCLAVSVQYRRAPEDPVPAA 124

Query: 131 YEDSWAAVEWAASHSNHDGAEIWLNQHADFNRVFFVGDSAGGNICYNMVGRASESDNGLK 190
           YED W+A++W  +HSN  G   W+N+HADF +VF  GDSAGGNI ++M  +A +      
Sbjct: 125 YEDVWSAIQWIFAHSNGSGPVDWINKHADFGKVFLGGDSAGGNISHHMAMKAGKEKK--L 184

Query: 191 GMKIVGLGLVHPFFM----IDQPD-----------KLIEYI-FPTTV-CLEDPRLNPWVN 250
            +KI G+ +VHP F     +D+ D           ++ E I  P +V   +DP  N  VN
Sbjct: 185 DLKIKGIAVVHPAFWGTDPVDEYDVQDKETRSGIAEIWEKIASPNSVNGTDDPLFN--VN 244

Query: 251 ---PRLGKLRCERVLIMAAELDDLKDRAHGFHAALRESGFEGSVEIVETEGEIHVFHLFY 305
                   L C++VL+  A  D    +   + A L +  +EG+VE+VE EGE HVFHL  
Sbjct: 245 GSGSDFSGLGCDKVLVAVAGKDVFVRQGLAYAAKLEKCEWEGTVEVVEEEGEDHVFHLQN 304

[Close]

BLAST of Lsi10G003030 vs. NCBI nr
Match: gi|470117387|ref|XP_004294840.1| (PREDICTED: probable carboxylesterase 7 [Fragaria vesca subsp. vesca])

HSP 1 Score: 340.5 bits (872), Expect = 3.0e-90
Identity = 168/300 (56.00%), Postives = 214/300 (71.33%), Query Frame = 1


Query: 5   AAAGDGVVHEFPPAFCVLKSGRIQRLFSDERLPPSTDPLTGVQSKDILIDPLTQLFVRIY 64
           A   D +  EFPP F + K GR  RL  +E +PPSTDP TGVQSKDI++ P + L  RIY
Sbjct: 4   ATQNDEIAFEFPPLFRLYKDGRADRLKGNETVPPSTDPTTGVQSKDIVLSPESGLSARIY 63

Query: 65  LPNVSDSTKKLPLLIYIHGGAFSIGSVTAPMYHYHLISLAAEANAIALSVEYRLAPEHPL 124
           LP + D T+KLP L+YIHGG F I S ++P+YH H+ SLAA+AN +ALS+ YR APEHPL
Sbjct: 64  LPKLPDPTRKLPFLLYIHGGGFVIESPSSPVYHSHVSSLAAQANVVALSIHYRRAPEHPL 123

Query: 125 PAAYEDSWAAVEWAASHSNHDGAEIWLNQHADFNRVFFVGDSAGGNICYNMVGRASESDN 184
           PAA+EDSWAA +WAA+HS   G E WLN+HADFNRVF  GDSAG  + +++V ++     
Sbjct: 124 PAAFEDSWAAFQWAATHSTGGGPESWLNEHADFNRVFVAGDSAGSTLAHHVVRQSGV--E 183

Query: 185 GLKGMKIVGLGLVHPFFMIDQPDKLIEYIFPTTVCLEDPRLNPWVNPRLGKLRCERVLIM 244
           G+ G+  VG+ L HP+F  + PDKL+E I+PT   LEDP++ P  +PRLG L   RVL+ 
Sbjct: 184 GVSGVNTVGVVLFHPYFRNEVPDKLLEVIYPTGGGLEDPKMYPGNDPRLGSLGAGRVLVF 243

Query: 245 AAELDDLKDRAHGFHAALRESGFEGSVEIVETEGEIHVFHLFYPEKEKAAALVKKVASFI 304
            AE D LKDR   ++ AL++SG+ G VEI ETEGE HVFHLF P  EKA +LVKKVA+F+
Sbjct: 244 VAEKDFLKDRGWSYYEALKKSGWSGVVEIEETEGEDHVFHLFDPSCEKAVSLVKKVAAFM 301

[Close]

BLAST of Lsi10G003030 vs. NCBI nr
Match: gi|694353183|ref|XP_009358071.1| (PREDICTED: probable carboxylesterase 12 [Pyrus x bretschneideri])

HSP 1 Score: 332.8 bits (852), Expect = 6.4e-88
Identity = 163/301 (54.15%), Postives = 214/301 (71.10%), Query Frame = 1


Query: 4   DAAAGDGVVHEFPPAFCVLKSGRIQRLFSDERLPPSTDPLTGVQSKDILIDPLTQLFVRI 63
           D+     V  EFP  F +   GR +RL   E +PPSTDP TGVQSKDI++ P + L  R+
Sbjct: 2   DSKPSSEVSFEFPTVFRIYNDGRTERLKGTETVPPSTDPTTGVQSKDIVLSPQSGLSARV 61

Query: 64  YLPNVSDSTKKLPLLIYIHGGAFSIGSVTAPMYHYHLISLAAEANAIALSVEYRLAPEHP 123
           +LP + D T+KLPLLI+IHGGAF I S  +P+YH H+  LA+EAN +ALSV YR APEHP
Sbjct: 62  FLPKLPDPTRKLPLLIFIHGGAFVIESPYSPLYHNHVGLLASEANVVALSVHYRRAPEHP 121

Query: 124 LPAAYEDSWAAVEWAASHSNHDGAEIWLNQHADFNRVFFVGDSAGGNICYNMVGRASESD 183
           LP A+EDSW AV+WAA+HS+ +G E WLN H DF+RVF  GDSAG  + +N+V +A    
Sbjct: 122 LPVAFEDSWDAVQWAAAHSSGNGPEAWLNDHVDFDRVFIGGDSAGATLTHNVVRQAGL-- 181

Query: 184 NGLKGMKIVGLGLVHPFFMIDQPDKLIEYIFPTTVCLEDPRLNPWVNPRLGKLRCERVLI 243
           +GL G +IVG+ L HP+FM D+PDKL+E I+PT    +DPR+ P  +P+LG++ C RVL+
Sbjct: 182 DGLTGARIVGMILFHPYFMDDEPDKLLEVIYPTCGGSDDPRVRPGNDPKLGEIGCGRVLV 241

Query: 244 MAAELDDLKDRAHGFHAALRESGFEGSVEIVETEGEIHVFHLFYPEKEKAAALVKKVASF 303
             AE D L+DR   ++ AL++SG+ G VEIVE+EGE HVFHLF P  + +  LVKKV SF
Sbjct: 242 FVAEKDFLRDRGWAYYEALKKSGYGGVVEIVESEGEDHVFHLFNPSCDNSVDLVKKVVSF 300

Query: 304 I 305
           +
Sbjct: 302 V 300

[Close]

BLAST of Lsi10G003030 vs. NCBI nr
Match: gi|82697941|gb|ABB89005.1| (CXE carboxylesterase [Malus pumila])

HSP 1 Score: 331.3 bits (848), Expect = 1.8e-87
Identity = 162/301 (53.82%), Postives = 212/301 (70.43%), Query Frame = 1


Query: 4   DAAAGDGVVHEFPPAFCVLKSGRIQRLFSDERLPPSTDPLTGVQSKDILIDPLTQLFVRI 63
           D+     V  EFP AF +   GR +R    E +PPSTD  TGVQ KDI++ P + L  R+
Sbjct: 2   DSKPSSEVSFEFPTAFRIYNDGRTERFKGIETVPPSTDSTTGVQCKDIVLSPQSGLSARV 61

Query: 64  YLPNVSDSTKKLPLLIYIHGGAFSIGSVTAPMYHYHLISLAAEANAIALSVEYRLAPEHP 123
           +LP + D T+KLPLLI+IHGGAF I S  +P+YH H++ LA+EAN +ALSV YR APEHP
Sbjct: 62  FLPKLPDPTRKLPLLIFIHGGAFVIESPYSPLYHKHVMLLASEANVVALSVHYRRAPEHP 121

Query: 124 LPAAYEDSWAAVEWAASHSNHDGAEIWLNQHADFNRVFFVGDSAGGNICYNMVGRASESD 183
           LP A+EDSW AVEWAA+HS  +G E WLN H DF+RVF  GDSAG  + +++V +A    
Sbjct: 122 LPVAFEDSWDAVEWAAAHSTRNGPEAWLNDHVDFDRVFIGGDSAGATLTHHVVRQAGL-- 181

Query: 184 NGLKGMKIVGLGLVHPFFMIDQPDKLIEYIFPTTVCLEDPRLNPWVNPRLGKLRCERVLI 243
           +GL G +IVG+ L HP+FM D+PDKL+E I+PT    +DPR+ P  +P+LG++ C RVL+
Sbjct: 182 DGLSGTRIVGMILFHPYFMDDEPDKLLEVIYPTCGGSDDPRVRPGNDPKLGEIGCGRVLV 241

Query: 244 MAAELDDLKDRAHGFHAALRESGFEGSVEIVETEGEIHVFHLFYPEKEKAAALVKKVASF 303
             AE D L+DR   +H AL++SG+ G VEIVE++GE HVFHLF P  + A  LVKKV SF
Sbjct: 242 FVAEKDFLRDRGWAYHEALKKSGYGGVVEIVESQGEDHVFHLFNPSCDNAVDLVKKVVSF 300

Query: 304 I 305
           +
Sbjct: 302 V 300

[Close]

BLAST of Lsi10G003030 vs. NCBI nr
Match: gi|657999750|ref|XP_008392308.1| (PREDICTED: probable carboxylesterase 7 [Malus domestica])

HSP 1 Score: 329.7 bits (844), Expect = 5.4e-87
Identity = 162/301 (53.82%), Postives = 211/301 (70.10%), Query Frame = 1


Query: 4   DAAAGDGVVHEFPPAFCVLKSGRIQRLFSDERLPPSTDPLTGVQSKDILIDPLTQLFVRI 63
           D+     V  EFP AF +   GR +R    E +PPSTD  TGVQ KDI++ P + L  R+
Sbjct: 60  DSKPSSEVSFEFPTAFRIYNDGRTERFKGIETVPPSTDSTTGVQCKDIVLSPQSGLSARV 119

Query: 64  YLPNVSDSTKKLPLLIYIHGGAFSIGSVTAPMYHYHLISLAAEANAIALSVEYRLAPEHP 123
           +LP + D T+KLPLLI+IHGGAF I S  +P+YH H+  LA+EAN +ALSV YR APEHP
Sbjct: 120 FLPKLPDPTRKLPLLIFIHGGAFVIESPYSPLYHKHVRLLASEANVVALSVHYRRAPEHP 179

Query: 124 LPAAYEDSWAAVEWAASHSNHDGAEIWLNQHADFNRVFFVGDSAGGNICYNMVGRASESD 183
           LP A+EDSW AVEWAA+HS  +G E WLN H DF+RVF  GDSAG  + +++V +A    
Sbjct: 180 LPVAFEDSWDAVEWAAAHSTRNGPEAWLNDHVDFDRVFIGGDSAGATLTHHVVRQAGL-- 239

Query: 184 NGLKGMKIVGLGLVHPFFMIDQPDKLIEYIFPTTVCLEDPRLNPWVNPRLGKLRCERVLI 243
           +GL G +IVG+ L HP+FM D+PDKL+E I+PT    +DPR+ P  +P+LG++ C RVL+
Sbjct: 240 DGLSGTRIVGMILFHPYFMDDEPDKLLEVIYPTCGGSDDPRVRPGNDPKLGEIGCGRVLV 299

Query: 244 MAAELDDLKDRAHGFHAALRESGFEGSVEIVETEGEIHVFHLFYPEKEKAAALVKKVASF 303
             AE D L+DR   +H AL++SG+ G VEIVE++GE HVFHLF P  + A  LVKKV SF
Sbjct: 300 FVAEKDFLRDRGWAYHEALKKSGYGGVVEIVESQGEDHVFHLFNPSCDNAVDLVKKVVSF 358

Query: 304 I 305
           +
Sbjct: 360 V 358

[Close]

BLAST of Lsi10G003030 vs. NCBI nr
Match: gi|694450813|ref|XP_009350720.1| (PREDICTED: probable carboxylesterase 12 [Pyrus x bretschneideri])

HSP 1 Score: 328.9 bits (842), Expect = 9.2e-87
Identity = 162/301 (53.82%), Postives = 213/301 (70.76%), Query Frame = 1


Query: 4   DAAAGDGVVHEFPPAFCVLKSGRIQRLFSDERLPPSTDPLTGVQSKDILIDPLTQLFVRI 63
           D+     V  EFP  F +   GR +RL   E +PPSTDP TGVQSKDI++ P + L  R+
Sbjct: 2   DSKPSSEVSFEFPTVFRIYNDGRTERLKGTETVPPSTDPTTGVQSKDIVLSPQSGLSARV 61

Query: 64  YLPNVSDSTKKLPLLIYIHGGAFSIGSVTAPMYHYHLISLAAEANAIALSVEYRLAPEHP 123
           +LP + D T+KLPLLI+IHGGAF I S  +P+YH H+  LA+EAN +ALSV YR APEHP
Sbjct: 62  FLPKLPDPTRKLPLLIFIHGGAFVIESPYSPLYHNHVGFLASEANVVALSVHYRRAPEHP 121

Query: 124 LPAAYEDSWAAVEWAASHSNHDGAEIWLNQHADFNRVFFVGDSAGGNICYNMVGRASESD 183
           LP A+EDSW AV+WAA+HS+ +G E WLN H DF+RVF  GDSAG  + +N+V +A    
Sbjct: 122 LPVAFEDSWDAVQWAAAHSSGNGPEAWLNDHVDFDRVFIGGDSAGATLTHNVVRQAGL-- 181

Query: 184 NGLKGMKIVGLGLVHPFFMIDQPDKLIEYIFPTTVCLEDPRLNPWVNPRLGKLRCERVLI 243
           +GL G +IVG+ L HP+FM D+PDKL+E I+PT    +DPR+ P  + +LG++ C RVL+
Sbjct: 182 DGLTGARIVGMILFHPYFMDDEPDKLLEVIYPTCGGSDDPRVRPGNDLKLGEIGCGRVLV 241

Query: 244 MAAELDDLKDRAHGFHAALRESGFEGSVEIVETEGEIHVFHLFYPEKEKAAALVKKVASF 303
             AE D L+DR   ++ AL++SG+ G VEIVE+EGE HVFHLF P  + +  LVKKV SF
Sbjct: 242 FVAEKDFLRDRGWAYYEALKKSGYGGVVEIVESEGEDHVFHLFNPSCDNSVNLVKKVVSF 300

Query: 304 I 305
           +
Sbjct: 302 V 300

The following BLAST results are available for this feature:

BLAST of Lsi10G003030 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CXE2_ARATH	3.5e-62	42.90	Probable carboxylesterase 2 OS=Arabidopsis thaliana GN=CXE2 PE=2 SV=1	[more]
CXE12_ARATH	3.6e-59	41.16	Probable carboxylesterase 12 OS=Arabidopsis thaliana GN=CXE12 PE=1 SV=1	[more]
CXE13_ARATH	6.8e-58	39.44	Probable carboxylesterase 13 OS=Arabidopsis thaliana GN=CXE13 PE=1 SV=1	[more]
HIDM_GLYEC	6.8e-58	36.92	2-hydroxyisoflavanone dehydratase OS=Glycyrrhiza echinata GN=HIDM PE=1 SV=1	[more]
CXE1_ARATH	7.6e-57	39.03	Probable carboxylesterase 1 OS=Arabidopsis thaliana GN=CXE1 PE=2 SV=1	[more]

BLAST of Lsi10G003030 vs. TrEMBL


Match Name	E-value	Identity	Description
Q0ZPW5_MALPM	1.3e-87	53.82	CXE carboxylesterase OS=Malus pumila GN=CXE5 PE=2 SV=1	[more]
M5VQ13_PRUPE	2.4e-86	55.33	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa009160mg PE=4 SV=1	[more]
B9HRY0_POPTR	3.0e-84	52.58	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0009s10800g PE=4 SV=2	[more]
A0A061E1M8_THECC	2.1e-82	52.94	Alpha/beta-Hydrolases superfamily protein, putative OS=Theobroma cacao GN=TCM_00...	[more]
W9SMM8_9ROSA	6.2e-82	54.88	Putative carboxylesterase 7 OS=Morus notabilis GN=L484_027556 PE=4 SV=1	[more]

BLAST of Lsi10G003030 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G47480.1	2.0e-63	42.90	alpha/beta-Hydrolases superfamily protein	[more]
AT3G48690.1	2.0e-60	41.16	alpha/beta-Hydrolases superfamily protein	[more]
AT3G48700.1	3.9e-59	39.44	carboxyesterase 13	[more]
AT1G19190.1	4.3e-58	39.03	alpha/beta-Hydrolases superfamily protein	[more]
AT1G49660.1	1.2e-57	41.01	carboxyesterase 5	[more]

BLAST of Lsi10G003030 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|470117387\|ref\|XP_004294840.1\|	3.0e-90	56.00	PREDICTED: probable carboxylesterase 7 [Fragaria vesca subsp. vesca]	[more]
gi\|694353183\|ref\|XP_009358071.1\|	6.4e-88	54.15	PREDICTED: probable carboxylesterase 12 [Pyrus x bretschneideri]	[more]
gi\|82697941\|gb\|ABB89005.1\|	1.8e-87	53.82	CXE carboxylesterase [Malus pumila]	[more]
gi\|657999750\|ref\|XP_008392308.1\|	5.4e-87	53.82	PREDICTED: probable carboxylesterase 7 [Malus domestica]	[more]
gi\|694450813\|ref\|XP_009350720.1\|	9.2e-87	53.82	PREDICTED: probable carboxylesterase 12 [Pyrus x bretschneideri]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0016787	hydrolase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR013094	AB_hydrolase_3
IPR002168	Lipase_GDXG_HIS_AS

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016787	hydrolase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi10G003030.1	Lsi10G003030.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002168	Lipase, GDXG, putative histidine active site	PROSITE	PS01173	LIPASE_GDXG_HIS	coord: 78..93 scor
IPR013094	Alpha/beta hydrolase fold-3	PFAM	PF07859	Abhydrolase_3	coord: 78..284 score: 2.0
None	No IPR available	PANTHER	PTHR23024	MEMBER OF 'GDXG' FAMILY OF LIPOLYTIC ENZYMES	coord: 11..304 score: 1.6E
None	No IPR available	PANTHER	PTHR23024:SF131	CARBOXYLESTERASE 2-RELATED	coord: 11..304 score: 1.6E

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi10G003030	Cucumber (Gy14) v1	cgylsiB092
Lsi10G003030	Cucurbita maxima (Rimu)	cmalsiB507
Lsi10G003030	Cucurbita maxima (Rimu)	cmalsiB548
Lsi10G003030	Cucurbita moschata (Rifu)	cmolsiB491
Lsi10G003030	Cucurbita moschata (Rifu)	cmolsiB533
Lsi10G003030	Cucurbita pepo (Zucchini)	cpelsiB220
Lsi10G003030	Cucurbita pepo (Zucchini)	cpelsiB589
Lsi10G003030	Wild cucumber (PI 183967)	cpilsiB091
Lsi10G003030	Cucumber (Chinese Long) v2	culsiB093
Lsi10G003030	Melon (DHL92) v3.5.1	lsimeB057
Lsi10G003030	Watermelon (Charleston Gray)	lsiwcgB034
Lsi10G003030	Watermelon (97103) v1	lsiwmB043
Lsi10G003030	Cucumber (Gy14) v2	cgyblsiB086
Lsi10G003030	Melon (DHL92) v3.6.1	lsimedB059
Lsi10G003030	Silver-seed gourd	carlsiB731
Lsi10G003030	Silver-seed gourd	carlsiB961
Lsi10G003030	Cucumber (Chinese Long) v3	cuclsiB104
Lsi10G003030	Watermelon (97103) v2	lsiwmbB070
Lsi10G003030	Wax gourd	lsiwgoB074