Lsi10G002920 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi10G002920
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Carboxylesterase 1
Location	chr10 : 4755578 .. 4760305 (-)

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Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATAATACAACTACTTCCACATTGAAATCTCAGCCCCTTCTTATATTTATTTATATCCTTACACGAATACTTAACAAACCAAACCACCATTTTTCTTATTTCTTATTTTGTCTTCTTCACCTCATCAATGGCTTCTTCACCAAACAAAAATGACGATGTAGCCTACGACTTCTTTCCCTTCGTCAGAATCTACAAAGACGGTCGCGTCCAAAGACTCCAAATCACCTCTTACATTGTTTCTGCTGACGCCGATGACCCCAAATCTGCTTTTCTATCAAAAGATGTGACGATCTCGGCGGATCCCGCCGTGTCGGCGCGTATATTCATCCCCTCTTTCGCTGATCCAAATCAGAAGCTGCCCATCCTCTTGTACGTCCACGGCGGAGGGTTCTGCATCGAATCGGCTTTCAATTTGCAGTACCACAGCTACGTGGGTTCTCTTGCTGTCAAGGCTAATGTCGTCGCTGTCTCTGTTGAGTACCGCCTCGCGCCGGAGCATCTGTTCCCCGCCTGTTACGAGGACTGTTGGGATGCTTTACGGTGGGTTGCGACACATGTGAACAGGTAATTAACGAGGGAAAGGAAAAAAATTAATCATTTTCTTAAAAAAAACCTAACACATGTCAATTTTTAACTATGCACCGCCTAGGGTTGGTGAAGAAGAGAAAGATAGGGGAGGGGGTTGGCGAAGAAGAAGATGAGGCGTGAGTTTATTAGAATAGGTTGAAGGAGAGATAAGACAAAAGAGAATAAGATAATGGAGAAAGAAAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTAATGGCAAAATACCATTATAGTTTCCATATTATTGAATTTTAGTCGTTATACTTTCTAATTTTAGTTTTTATACTTTTAATAAATCTTAAATTTAACTACTTTCTCATACTAATTAATTATAGATTTTAAGAAGAAAAAAATAATATTTAAAATGTGTCATTTAACTCTAAGGACTAGAATCAACCATTTATAAAATGTTAGGGATTTGAATAATTGAATGGAAACTTTTTAAAAATAAAAAAATAAAAGAAACTATTTACATTAAATAGCAAAATTTTTAGATAGTTGGATAGATGATGATAGAAGTCTATCAGGATCTATTGGTGATAGAAATGATAGAAGTCTATCAGGATCTATTGGTGATAGAAATGATAGAAGTCTATCACTGATAGATGCTGAAGTCTATCAATGTCTATCAGTGATTTTTTTTGCTATTTTCTGTAAATAGTTTGATATTTTTTCTATTTGTGAAAATTTTCCTAATTGAATGTGCTTGAATATGATAATGGAAGCTAAGTTTTAATTTCCATGTAAATTGATTTTTTTTTTAAAAGAAAAAAACTGAACAATTTTAATATGATAGAGATATGTATGATTTAACTAAACTATATTCTTTTTGAAAAAAAATCATATTAAAGCTTTTAAAATTATATAGATATCAAATTAAAACTAAATCCAAATTTATAACTTTGAAGACTATTAGTTTAAATTGGGAGTTTGATCCAAAATTATAGTGTATGCAATCCTTAGCTTAAGTTGAATTAGTAATTACTATTATTATAGGTATTAATTCAGAAGTGTAGCTTTTATTACTTAACTTATGAAGTATACTATATACATACATATAACTTATTATTTTCTTTCCCTCTCTTTTAAATTTTAACCAAGGTTTAAAAGGTTGAAGTCGAAGGAATATTGGAAGCCTTAGTTTTACGAAATTATTGATAAAATATTGATTTTGATGAATGTTTATGAAAGAATTAAAAATAAAAAATTCAAAAAATAAATTTAGATTTAGTAAATAAACATTTTATATATTTAAAAGTCAAATTATTTATATTATATTTGCATTATTGACATTTTGTTGCTTGTTTTTTATATTTCGTAGATTTTTCTACGATACAATAGAAATATCAAATTCACCCCTAATATTTATATTGATGTTGAATCTATGGAAACAAAAAAATATCGATGGAAATATCGATATACCGATAGAAATCTAATACTATGATTTTAACCTTAGTATTTTTTTAGTACAATATCGGTGCGAAAATTTGTGATTACTATATATTAGTTGAGCTACATCAATCTCCTGTGCCAATAGGGATGGTCCAGAGCCGTGGCTAAACAACCATGCAGATTTCAATCGAGTCTGTTTGGCCGGCGACAGTGCCGGTGCCAACATCTGCAATCATTTGGCTGTGCGAGCAGGTTCATCAGCAGAGCAACTGGGCGGAATAAAGGTGGTGGCAATGGCTTTGATTCACCCGTTCTTCGGTGACGGGGGAGATGATAGGCTATGGAAGTATCTGTGTTCGGATCAGACGAGGTCGGCGAGGCCAAGGACAGAGGATTTGGCAAAGTTGCCATGTGGAAGAGTGAAGGTTTTTTTGGCAGAAGAAGATTTTTTGAAAGTTGGAGGGAGGAATTACGGTGAGGATTTGAAGAGGAGCAGGTGGAATGGGGCGGTGGAGACGGTGGAGCACGATGGAGAAGATCATGTGTTTCATTTGAAGAAGCCAGAGTGTGAAAAGGCACTTGATTTGTTGGAAAAATTAGCCTCTTTCATCAACTTAGATTAGAACATAATTTATGTTCATTACTAATATTTGAAAAACTATTAAATAATTATATATCGAAAACTTTAATGTATTACTCTTCAAATAAATCTTAGATTAAAATTAACATTTTGTTGGTCTTTTGTATTTGAATCAGACTTATTCTATTTCTCTATCTCTCTCACTATATTTACTTAGAATGGGAATATATATATAGTATAAATCTATATCTAT

mRNA sequence

AATAATACAACTACTTCCACATTGAAATCTCAGCCCCTTCTTATATTTATTTATATCCTTACACGAATACTTAACAAACCAAACCACCATTTTTCTTATTTCTTATTTTGTCTTCTTCACCTCATCAATGGCTTCTTCACCAAACAAAAATGACGATGTAGCCTACGACTTCTTTCCCTTCGTCAGAATCTACAAAGACGGTCGCGTCCAAAGACTCCAAATCACCTCTTACATTGTTTCTGCTGACGCCGATGACCCCAAATCTGCTTTTCTATCAAAAGATGTGACGATCTCGGCGGATCCCGCCGTGTCGGCGCGTATATTCATCCCCTCTTTCGCTGATCCAAATCAGAAGCTGCCCATCCTCTTGTACGTCCACGGCGGAGGGTTCTGCATCGAATCGGCTTTCAATTTGCAGTACCACAGCTACGTGGGTTCTCTTGCTGTCAAGGCTAATGTCGTCGCTGTCTCTGTTGAGTACCGCCTCGCGCCGGAGCATCTGTTCCCCGCCTGTTACGAGGACTGTTGGGATGCTTTACGGTGGGTTGCGACACATGTGAACAGGGATGGTCCAGAGCCGTGGCTAAACAACCATGCAGATTTCAATCGAGTCTGTTTGGCCGGCGACAGTGCCGGTGCCAACATCTGCAATCATTTGGCTGTGCGAGCAGGTTCATCAGCAGAGCAACTGGGCGGAATAAAGGTGGTGGCAATGGCTTTGATTCACCCGTTCTTCGGTGACGGGGGAGATGATAGGCTATGGAAGTATCTGTGTTCGGATCAGACGAGGTCGGCGAGGCCAAGGACAGAGGATTTGGCAAAGTTGCCATGTGGAAGAGTGAAGGTTTTTTTGGCAGAAGAAGATTTTTTGAAAGTTGGAGGGAGGAATTACGGTGAGGATTTGAAGAGGAGCAGGTGGAATGGGGCGGTGGAGACGGTGGAGCACGATGGAGAAGATCATGTGTTTCATTTGAAGAAGCCAGAGTGTGAAAAGGCACTTGATTTGTTGGAAAAATTAGCCTCTTTCATCAACTTAGATTAGAACATAATTTATGTTCATTACTAATATTTGAAAAACTATTAAATAATTATATATCGAAAACTTTAATGTATTACTCTTCAAATAAATCTTAGATTAAAATTAACATTTTGTTGGTCTTTTGTATTTGAATCAGACTTATTCTATTTCTCTATCTCTCTCACTATATTTACTTAGAATGGGAATATATATATAGTATAAATCTATATCTAT

Coding sequence (CDS)

ATGGCTTCTTCACCAAACAAAAATGACGATGTAGCCTACGACTTCTTTCCCTTCGTCAGAATCTACAAAGACGGTCGCGTCCAAAGACTCCAAATCACCTCTTACATTGTTTCTGCTGACGCCGATGACCCCAAATCTGCTTTTCTATCAAAAGATGTGACGATCTCGGCGGATCCCGCCGTGTCGGCGCGTATATTCATCCCCTCTTTCGCTGATCCAAATCAGAAGCTGCCCATCCTCTTGTACGTCCACGGCGGAGGGTTCTGCATCGAATCGGCTTTCAATTTGCAGTACCACAGCTACGTGGGTTCTCTTGCTGTCAAGGCTAATGTCGTCGCTGTCTCTGTTGAGTACCGCCTCGCGCCGGAGCATCTGTTCCCCGCCTGTTACGAGGACTGTTGGGATGCTTTACGGTGGGTTGCGACACATGTGAACAGGGATGGTCCAGAGCCGTGGCTAAACAACCATGCAGATTTCAATCGAGTCTGTTTGGCCGGCGACAGTGCCGGTGCCAACATCTGCAATCATTTGGCTGTGCGAGCAGGTTCATCAGCAGAGCAACTGGGCGGAATAAAGGTGGTGGCAATGGCTTTGATTCACCCGTTCTTCGGTGACGGGGGAGATGATAGGCTATGGAAGTATCTGTGTTCGGATCAGACGAGGTCGGCGAGGCCAAGGACAGAGGATTTGGCAAAGTTGCCATGTGGAAGAGTGAAGGTTTTTTTGGCAGAAGAAGATTTTTTGAAAGTTGGAGGGAGGAATTACGGTGAGGATTTGAAGAGGAGCAGGTGGAATGGGGCGGTGGAGACGGTGGAGCACGATGGAGAAGATCATGTGTTTCATTTGAAGAAGCCAGAGTGTGAAAAGGCACTTGATTTGTTGGAAAAATTAGCCTCTTTCATCAACTTAGATTAG

Protein sequence

MASSPNKNDDVAYDFFPFVRIYKDGRVQRLQITSYIVSADADDPKSAFLSKDVTISADPAVSARIFIPSFADPNQKLPILLYVHGGGFCIESAFNLQYHSYVGSLAVKANVVAVSVEYRLAPEHLFPACYEDCWDALRWVATHVNRDGPEPWLNNHADFNRVCLAGDSAGANICNHLAVRAGSSAEQLGGIKVVAMALIHPFFGDGGDDRLWKYLCSDQTRSARPRTEDLAKLPCGRVKVFLAEEDFLKVGGRNYGEDLKRSRWNGAVETVEHDGEDHVFHLKKPECEKALDLLEKLASFINLD

Homology

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BLAST of Lsi10G002920 vs. Swiss-Prot
Match: CXE12_ARATH (Probable carboxylesterase 12 OS=Arabidopsis thaliana GN=CXE12 PE=1 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 2.0e-62
Identity = 130/321 (40.50%), Postives = 190/321 (59.19%), Query Frame = 1


Query: 8   NDDVAYDFFPFVRIYKDGRVQRLQITSYIVSADADDPKSAFLSKDVTISADPAVSARIFI 67
           + ++A D  P ++IYK GR++RL   + +    + +P++  +SKDV  SAD  +S RI++
Sbjct: 2   DSEIAVDCSPLLKIYKSGRIERLMGEATV--PPSSEPQNGVVSKDVVYSADNNLSVRIYL 61

Query: 68  P--SFADPNQKLPILLYVHGGGFCIESAFNLQYHSYVGSLAVKANVVAVSVEYRLAPEHL 127
           P  + A+ + KLP+L+Y HGGGF IE+AF+  YH+++ +    +N VAVSV+YR APEH 
Sbjct: 62  PEKAAAETDSKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPEHP 121

Query: 128 FPACYEDCWDALRWVATHVNRDGPEPWLNNHADFNRVCLAGDSAGANICNHLAVRAGSS- 187
               ++D W AL+WV TH+   G E WLN HADF+RV L+GDSAGANI +H+A+RA    
Sbjct: 122 ISVPFDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKEK 181

Query: 188 -AEQLGGIKVVAMALIHPFFG-----DGGD--DRLWKYLCSDQTRSARPRTE-------- 247
            +  L    +  + L+HP+F      D  D  D   +         A P ++        
Sbjct: 182 LSPGLNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDPLL 241

Query: 248 --------DLAKLPCGRVKVFLAEEDFLKVGGRNYGEDLKRSRWNGAVETVEHDGEDHVF 302
                   DL+ L CG+V V +AE+D L   G  Y   L++S W G VE VE +GEDHVF
Sbjct: 242 NVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDHVF 301

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BLAST of Lsi10G002920 vs. Swiss-Prot
Match: CXE1_ARATH (Probable carboxylesterase 1 OS=Arabidopsis thaliana GN=CXE1 PE=2 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 7.2e-60
Identity = 120/314 (38.22%), Postives = 178/314 (56.69%), Query Frame = 1


Query: 8   NDDVAYDFFPFVRIYKDGRVQRLQITSYIVSADADDPKSAFLSKDVTISADPAVSARIFI 67
           + ++A+D+ P  RI+K+G ++RL   +++    + +P++  +SKD   S +  +S RI++
Sbjct: 2   DSEIAFDYSPRFRIFKNGGIERLVPETFV--PPSLNPENGVVSKDAVYSPEKNLSLRIYL 61

Query: 68  PS---FADPNQKLPILLYVHGGGFCIESAFNLQYHSYVGSLAVKANVVAVSVEYRLAPEH 127
           P    +    +K+P+L+Y HGGGF +E+AF+  YH+++ S     + +AVSVEYR APEH
Sbjct: 62  PQNSVYETGEKKIPLLVYFHGGGFIMETAFSPIYHTFLTSAVSATDCIAVSVEYRRAPEH 121

Query: 128 LFPACYEDCWDALRWVATHVNRDGPEPWLNNHADFNRVCLAGDSAGANICNHLAVRAGSS 187
             P  YED WDA++W+ TH+ R GPE WLN HADF++V LAGDSAGANI +H+A+R    
Sbjct: 122 PIPTLYEDSWDAIQWIFTHITRSGPEDWLNKHADFSKVFLAGDSAGANIAHHMAIRVDKE 181

Query: 188 AEQLGGIKVVAMALIHPFFGDGG------------DDRLWKYLCSDQTRSARPR-----T 247
                  K+  M L HP+F                 +RLW+    D              
Sbjct: 182 KLPPENFKISGMILFHPYFLSKALIEEMEVEAMRYYERLWRIASPDSGNGVEDPWINVVG 241

Query: 248 EDLAKLPCGRVKVFLAEEDFLKVGGRNYGEDLKRSRWNGAVETVEHDGEDHVFHLKKPEC 302
            DL  L C RV V +A  D L  GG +Y  +L++S W G V+ +E   E HVFHL+ P+ 
Sbjct: 242 SDLTGLGCRRVLVMVAGNDVLARGGWSYVAELEKSGWIGKVKVMETKEEGHVFHLRDPDS 301

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BLAST of Lsi10G002920 vs. Swiss-Prot
Match: CXE5_ARATH (Probable carboxylesterase 5 OS=Arabidopsis thaliana GN=CXE5 PE=2 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 9.5e-60
Identity = 131/320 (40.94%), Postives = 183/320 (57.19%), Query Frame = 1


Query: 10  DVAYDFFPFVRIYKDGRVQRLQITSYIVSADADDPKSAFLSKDVTISADPAVSARIFIPS 69
           ++A +F PF RIYKDGRV+RL  T  I ++   DP    +SKDV  S +  +S R+F+P 
Sbjct: 4   EIASEFLPFCRIYKDGRVERLIGTDTIPASL--DPTYDVVSKDVIYSPENNLSVRLFLPH 63

Query: 70  FADP---NQKLPILLYVHGGGFCIESAFNLQYHSYVGSLAVKANVVAVSVEYRLAPEHLF 129
            +       KLP+L+Y+HGG + IES F+  YH+Y+  +   AN +AVSV+YR APE   
Sbjct: 64  KSTKLTAGNKLPLLIYIHGGAWIIESPFSPLYHNYLTEVVKSANCLAVSVQYRRAPEDPV 123

Query: 130 PACYEDCWDALRWVATHVNRDGPEPWLNNHADFNRVCLAGDSAGANICNHLAVRAGSSAE 189
           PA YED W A++W+  H N  GP  W+N HADF +V L GDSAG NI +H+A++AG   E
Sbjct: 124 PAAYEDVWSAIQWIFAHSNGSGPVDWINKHADFGKVFLGGDSAGGNISHHMAMKAGK--E 183

Query: 190 QLGGIKVVAMALIHPFFGDGGDDRLWKYLCSD-QTRS---------ARPRT--------- 249
           +   +K+  +A++HP F   G D + +Y   D +TRS         A P +         
Sbjct: 184 KKLDLKIKGIAVVHPAF--WGTDPVDEYDVQDKETRSGIAEIWEKIASPNSVNGTDDPLF 243

Query: 250 ------EDLAKLPCGRVKVFLAEEDFLKVGGRNYGEDLKRSRWNGAVETVEHDGEDHVFH 302
                  D + L C +V V +A +D     G  Y   L++  W G VE VE +GEDHVFH
Sbjct: 244 NVNGSGSDFSGLGCDKVLVAVAGKDVFVRQGLAYAAKLEKCEWEGTVEVVEEEGEDHVFH 303

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BLAST of Lsi10G002920 vs. Swiss-Prot
Match: HIDM_GLYEC (2-hydroxyisoflavanone dehydratase OS=Glycyrrhiza echinata GN=HIDM PE=1 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 1.2e-59
Identity = 122/326 (37.42%), Postives = 191/326 (58.59%), Query Frame = 1


Query: 2   ASSPNKNDDVAYDFFPFVRIYKDGRVQRLQITSYIVSADADDPKSAFLSKDVTISADPAV 61
           ++S   + ++  +  P +R+YKDG V+R   +S+ V    +DP++   +KD+ IS +P +
Sbjct: 4   STSTTTSKEIDRELPPLLRVYKDGTVERFLGSSF-VPPSPEDPETGVSTKDIVISENPTI 63

Query: 62  SARIFIPSFADPNQKLPILLYVHGGGFCIESAFNLQYHSYVGSLAVKANVVAVSVEYRLA 121
           SAR+++P   +  +KLPIL+Y HGG FC+ESAF+  +  Y+  +A KANV+ VS+EYRLA
Sbjct: 64  SARVYLPKLNNTTEKLPILVYYHGGAFCLESAFSFLHQRYLNIVASKANVLVVSIEYRLA 123

Query: 122 PEHLFPACYEDCWDALRWVATH-VNRDGP---EPWLNNHADFNRVCLAGDSAGANICNHL 181
           PEH  PA YED W AL+WV +H  N + P   +PWL  H DFNR  + GD++GANI ++ 
Sbjct: 124 PEHPLPAAYEDGWYALKWVTSHSTNNNKPTNADPWLIKHGDFNRFYIGGDTSGANIAHNA 183

Query: 182 AVRAGSSAEQLGGIKVVAMALIHPFFG----------DGGDD----RLWKYLCSDQ---- 241
           A+R G+ A   GG+++  +    P F           +G +     ++W ++  D     
Sbjct: 184 ALRVGAEALP-GGLRIAGVLSAFPLFWGSKPVLSEPVEGHEKSSPMQVWNFVYPDAPGGI 243

Query: 242 ----TRSARPRTEDLAKLPCGRVKVFLAEEDFLKVGGRNYGEDLKRSRWNGAVETVEHDG 301
                    P   +LA L C ++ VF+A +D L+  G  Y E +K S W G VE  +++G
Sbjct: 244 DNPLINPLAPGAPNLATLGCPKMLVFVAGKDDLRDRGIWYYEAVKESGWKGDVELAQYEG 303

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BLAST of Lsi10G002920 vs. Swiss-Prot
Match: CXE13_ARATH (Probable carboxylesterase 13 OS=Arabidopsis thaliana GN=CXE13 PE=1 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 6.1e-59
Identity = 130/330 (39.39%), Postives = 191/330 (57.88%), Query Frame = 1


Query: 8   NDDVAYDFFPFVRIYKDGRVQRLQITSYIVSADADDPKSAFLSKDVTISADPAVSARIFI 67
           + ++A D+ P + IYK GR++RL   + +    + +P++  +SKDV  S D  +S RI++
Sbjct: 2   DSEIAADYSPMLIIYKSGRIERLVGETTV--PPSSNPQNGVVSKDVVYSPDNNLSLRIYL 61

Query: 68  PSFA-----DPNQKLPILLYVHGGGFCIESAFNLQYHSYVGSLAVKANVVAVSVEYRLAP 127
           P  A     + + KLP+L+Y HGGGF +E+AF+  YH+++ +    ++ VAVSV+YR AP
Sbjct: 62  PEKAATAETEASVKLPLLVYFHGGGFLVETAFSPTYHTFLTAAVSASDCVAVSVDYRRAP 121

Query: 128 EHLFPACYEDCWDALRWVATHVNRDGPEPWLNNHADFNRVCLAGDSAGANICNHLAVRAG 187
           EH  P  Y+D W AL+WV +H+   G E WLN HADF++V LAGDSAGANI +H+ ++A 
Sbjct: 122 EHPIPTSYDDSWTALKWVFSHIAGSGSEDWLNKHADFSKVFLAGDSAGANITHHMTMKAA 181

Query: 188 S---SAEQLGGIKVVAMALIHPFFGDGG--DDR-------------LWKYLCSDQTRS-- 247
               S E L    +  + L+HP+F      DD+             +W  L S  ++   
Sbjct: 182 KDKLSPESLNESGISGIILVHPYFWSKTPVDDKETTDVAIRTWIESVWT-LASPNSKDGS 241

Query: 248 -------ARPRTEDLAKLPCGRVKVFLAEEDFLKVGGRNYGEDLKRSRWNGAV-ETVEHD 305
                   +  + DL+ L CG+V V +AE+D L   G  Y E L +SRWNG V + VE  
Sbjct: 242 DDPFINVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYWEKLGKSRWNGEVLDVVETK 301

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BLAST of Lsi10G002920 vs. TrEMBL
Match: A0A0A0LNZ1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G234530 PE=4 SV=1)

HSP 1 Score: 514.2 bits (1323), Expect = 1.1e-142
Identity = 246/304 (80.92%), Postives = 266/304 (87.50%), Query Frame = 1


Query: 1   MASSPNKNDDVAYDFFPFVRIYKDGRVQRLQITSYIVSADADDPKSAFLSKDVTISADPA 60
           MASS NKNDDVA+DFFPF+R+Y DGRVQRL  TS IV ADADDPKS F SKDVTIS DPA
Sbjct: 1   MASSTNKNDDVAFDFFPFLRVYTDGRVQRLMTTSDIVPADADDPKSPFRSKDVTISTDPA 60

Query: 61  VSARIFIPSFADPNQKLPILLYVHGGGFCIESAFNLQYHSYVGSLAVKANVVAVSVEYRL 120
           VSAR+FIPS ADPNQKLP+LLYVHGG FCIESAF+LQYH +VGSLA KAN VAVSVEYRL
Sbjct: 61  VSARVFIPSSADPNQKLPLLLYVHGGAFCIESAFSLQYHQHVGSLAAKANAVAVSVEYRL 120

Query: 121 APEHLFPACYEDCWDALRWVATHVNRDGPEPWLNNHADFNRVCLAGDSAGANICNHLAVR 180
           APEH  PACYEDCWDALRWVA HVNRDG EPWLN + DFNR+CLAGDSAGANIC++LA R
Sbjct: 121 APEHPIPACYEDCWDALRWVAAHVNRDGSEPWLNTYVDFNRICLAGDSAGANICHYLAAR 180

Query: 181 AGSSAEQLGGIKVVAMALIHPFFGDGGDDRLWKYLCSDQTRSARPRTEDLAKLPCGRVKV 240
           A SSAE+LGG KVVAMALIHPFFGDGG++RLWKYLCS +T+  RP  EDLAKL C RVK+
Sbjct: 181 ASSSAEELGGAKVVAMALIHPFFGDGGENRLWKYLCS-ETKLLRPTIEDLAKLGCKRVKI 240

Query: 241 FLAEEDFLKVGGRNYGEDLKRSRWNGAVETVEHDGEDHVFHLKKPECEKALDLLEKLASF 300
           FLAE DFLK GG+NY EDLK S WNG VETVEH  E+HVFHLKKPECEKA+DLLEKLASF
Sbjct: 241 FLAENDFLKSGGKNYEEDLKSSGWNGTVETVEHGEENHVFHLKKPECEKAVDLLEKLASF 300

Query: 301 INLD 305
           INLD
Sbjct: 301 INLD 303

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BLAST of Lsi10G002920 vs. TrEMBL
Match: F6HQD6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_03s0063g00810 PE=4 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 4.0e-89
Identity = 160/305 (52.46%), Postives = 213/305 (69.84%), Query Frame = 1


Query: 2   ASSPNKNDDVAYDFFPFVRIYKDGRVQRLQITSYIVSADADDPKSAFLSKDVTISADPAV 61
           A     N  +++ FFPF +IY+DGRV+R   T ++    +DDP +   SKDV IS +  V
Sbjct: 11  AKLEKSNGQLSHLFFPFFKIYQDGRVERFMHTDHV--PPSDDPLTGVRSKDVIISPETGV 70

Query: 62  SARIFIPSFADPNQKLPILLYVHGGGFCIESAFNLQYHSYVGSLAVKANVVAVSVEYRLA 121
           SAR+FIP   +PN KLP+L+Y+HGGGF I+SAF+  Y+ YV SL  +ANV+A+SV+YRLA
Sbjct: 71  SARLFIPKLPNPNCKLPLLIYIHGGGFSIQSAFSTSYNHYVKSLVAEANVIALSVDYRLA 130

Query: 122 PEHLFPACYEDCWDALRWVATHVNRDGPEPWLNNHADFNRVCLAGDSAGANICNHLAVRA 181
           PEH  PACY+D W A++W A+H N DGP+ WLNNHADF+RV  AGDSAG NI N LA R 
Sbjct: 131 PEHPIPACYDDSWAAVQWAASHANGDGPDTWLNNHADFSRVFFAGDSAGGNISNTLAFRV 190

Query: 182 GSSAEQLGGIKVVAMALIHPFFGDGGDDRLWKYLCSD----QTRSARPRTEDLAKLPCGR 241
           GSS   L G+KVV + L+HP+FG  GDD++W Y+C +    +    +P  EDLA+L C R
Sbjct: 191 GSSG--LPGVKVVGVVLVHPYFGGTGDDQMWLYMCPNHGGLEDPRLKPGAEDLARLGCER 250

Query: 242 VKVFLAEEDFLKVGGRNYGEDLKRSRWNGAVETVEHDGEDHVFHLKKPECEKALDLLEKL 301
           V +F+AE+D L+    +Y E LK+S W G VE VE+ GE+HVFHL  P+CE A  L++K+
Sbjct: 251 VLMFVAEKDHLRPVAWDYYEKLKKSEWKGTVEIVENHGEEHVFHLMNPKCENAAVLMKKI 310

Query: 302 ASFIN 303
            SF+N
Sbjct: 311 VSFLN 311

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BLAST of Lsi10G002920 vs. TrEMBL
Match: A8TU55_PAESU (CXE carboxylesterase OS=Paeonia suffruticosa PE=2 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 1.5e-88
Identity = 160/297 (53.87%), Postives = 208/297 (70.03%), Query Frame = 1


Query: 9   DDVAYDFFPFVRIYKDGRVQRLQITSYIVSADADDPKSAFLSKDVTISADPAVSARIFIP 68
           DDVA++FFPF R+YKDGRV+R   T  +     DDP +   SKDV IS  P V+ RIF+P
Sbjct: 33  DDVAHEFFPFFRVYKDGRVERFMPTEKV--PPTDDPNTGVRSKDVQIS--PEVAVRIFLP 92

Query: 69  SFADPNQKLPILLYVHGGGFCIESAFNLQYHSYVGSLAVKANVVAVSVEYRLAPEHLFPA 128
              DP QK+P+L Y HGGGF I SAF   YH+YV SL  +ANV+AVSV+YRLAPEH  PA
Sbjct: 93  KIDDPTQKVPVLFYTHGGGFSIGSAFAQGYHNYVSSLVAEANVIAVSVDYRLAPEHPIPA 152

Query: 129 CYEDCWDALRWVATHVNRDGPEPWLNNHADFNRVCLAGDSAGANICNHLAVRAGSSAEQL 188
           CYED W+A +WVA+H N +GPEPWLN+HADF RV + GDSAGANI + LA R GS+  +L
Sbjct: 153 CYEDSWEAFKWVASHANGNGPEPWLNDHADFRRVFMTGDSAGANITHTLAARIGST--EL 212

Query: 189 GGIKVVAMALIHPFFGDGGDDRLWKYLCSD----QTRSARPRTEDLAKLPCGRVKVFLAE 248
            G+KV+ +AL+HP+FG   DD++W +LC      +    +P TEDLAKL C ++ +F+A+
Sbjct: 213 PGVKVIGIALVHPYFGGTDDDKMWLFLCPTNGGLEDPRLKPATEDLAKLGCEKMLIFVAD 272

Query: 249 EDFLKVGGRNYGEDLKRSRWNGAVETVEHDGEDHVFHLKKPECEKALDLLEKLASFI 302
           ED LK  G +Y ++LK+S W G VE  E+ G+ HVFHL  P C+ A  + ++L SFI
Sbjct: 273 EDHLKERGISYYDELKKSGWKGTVEIEENKGQHHVFHLMNPTCDDAKAMKKRLVSFI 323

[Close]

BLAST of Lsi10G002920 vs. TrEMBL
Match: A0A059BCP2_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_G01495 PE=4 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 2.2e-87
Identity = 157/305 (51.48%), Postives = 211/305 (69.18%), Query Frame = 1


Query: 1   MASSPNKNDDVAYDFFPFVRIYKDGRVQRLQITSYIVSADADDPKSAFLSKDVTISADPA 60
           M  + + N DV ++FFPF ++YKDGRV+R+     +    ++D +    SKDVTIS +PA
Sbjct: 1   MEPTKSDNGDVLHEFFPFFKVYKDGRVERMMPMEKLKVPPSEDAEVR--SKDVTISTEPA 60

Query: 61  VSARIFIPSFADPNQKLPILLYVHGGGFCIESAFNLQYHSYVGSLAVKANVVAVSVEYRL 120
           V AR+F P    P ++LP+L+Y+HGGGF I SAF+  YH YV  L  +A VVAVSVEYRL
Sbjct: 61  VLARVFAPRGTGPAERLPVLIYIHGGGFSIGSAFHRVYHEYVSLLMARARVVAVSVEYRL 120

Query: 121 APEHLFPACYEDCWDALRWVATHVNRDGPEPWLNNHADFNRVCLAGDSAGANICNHLAVR 180
           APE+  PACY+D W A RW   H + +GP+PWLN H DF RV + GDSAGANIC+++AVR
Sbjct: 121 APENPIPACYDDAWAATRWALGHADGNGPDPWLNEHGDFGRVYITGDSAGANICHNVAVR 180

Query: 181 AGSSAEQLGGIKVVAMALIHPFFGDGGDDRLWKYLCSDQTRSARPR----TEDLAKLPCG 240
           AG  A    G+++  MAL+HP+FG   DD++W Y+C +    A PR     EDLA++ C 
Sbjct: 181 AG-WAGLPAGVRIAGMALVHPYFGGTDDDKMWLYMCPENGGLADPRLKPPPEDLARVGCE 240

Query: 241 RVKVFLAEEDFLKVGGRNYGEDLKRSRWNGAVETVEHDGEDHVFHLKKPECEKALDLLEK 300
           +V VF+A++D L+  G  Y E+LKRS W G+VE VEH+GE HVFH+ KP+C+KALDL +K
Sbjct: 241 KVLVFVAQKDHLRGRGLWYREELKRSGWGGSVEVVEHEGESHVFHILKPQCDKALDLKDK 300

Query: 301 LASFI 302
           L  F+
Sbjct: 301 LVDFL 302

[Close]

BLAST of Lsi10G002920 vs. TrEMBL
Match: M5W4H3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa009174mg PE=4 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 8.6e-84
Identity = 152/292 (52.05%), Postives = 204/292 (69.86%), Query Frame = 1


Query: 16  FPFVRIYKDGRVQRLQITSYIVSADADDPKSAFLSKDVTISADPAVSARIFIPSFADPNQ 75
           F F R YK+G+V++   T  I    +++P +   SKD+ IS +PA+SARIF+P   DP +
Sbjct: 14  FRFFRTYKNGQVEKFYSTHKI--PPSNNPVTGVQSKDIIISPEPAISARIFLPKIHDPTR 73

Query: 76  KLPILLYVHGGGFCIESAFNLQYHSYVGSLAVKANVVAVSVEYRLAPEHLFPACYEDCWD 135
           KLP+L Y+HGGGFC ESAF+  YH++V SL  +A V+AVSVEY L P+   PACYED W 
Sbjct: 74  KLPVLFYIHGGGFCFESAFSPLYHNHVASLVTEARVIAVSVEYGLYPDRPIPACYEDSWA 133

Query: 136 ALRWVATHVNRDGPEPWLNNHADFNRVCLAGDSAGANICNHLAVRAGSSAEQLGGIKVVA 195
           AL+WV +H  R+GPEPWLN +ADF+RV ++GDSAGANI ++LAVR G   +   G+KV  
Sbjct: 134 ALKWVVSHSARNGPEPWLNEYADFDRVFISGDSAGANISHNLAVRVG--PDGFSGVKVNG 193

Query: 196 MALIHPFFGD-GGDDRLWKYLCSD----QTRSARPRTEDLAKLPCGRVKVFLAEEDFLKV 255
           + L+HPFFG    DD +W Y+C +    Q R  +P  EDLA++ CGRV +F A ED L+ 
Sbjct: 194 IVLVHPFFGGLEEDDEMWLYMCPENGGLQDRRLKPPAEDLARIGCGRVLIFFAAEDHLRE 253

Query: 256 GGRNYGEDLKRSRWNGAVETVEHDGEDHVFHLKKPECEKALDLLEKLASFIN 303
            G+ Y ++L +S W G+VE VEH+GE HVFHL KP+ EKA +L++K  SFIN
Sbjct: 254 VGQRYYDELAKSEWGGSVEVVEHEGESHVFHLMKPDSEKATNLIKKFGSFIN 301

[Close]

BLAST of Lsi10G002920 vs. TAIR10
Match: AT3G48690.1 (AT3G48690.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 240.7 bits (613), Expect = 1.1e-63
Identity = 130/321 (40.50%), Postives = 190/321 (59.19%), Query Frame = 1


Query: 8   NDDVAYDFFPFVRIYKDGRVQRLQITSYIVSADADDPKSAFLSKDVTISADPAVSARIFI 67
           + ++A D  P ++IYK GR++RL   + +    + +P++  +SKDV  SAD  +S RI++
Sbjct: 2   DSEIAVDCSPLLKIYKSGRIERLMGEATV--PPSSEPQNGVVSKDVVYSADNNLSVRIYL 61

Query: 68  P--SFADPNQKLPILLYVHGGGFCIESAFNLQYHSYVGSLAVKANVVAVSVEYRLAPEHL 127
           P  + A+ + KLP+L+Y HGGGF IE+AF+  YH+++ +    +N VAVSV+YR APEH 
Sbjct: 62  PEKAAAETDSKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPEHP 121

Query: 128 FPACYEDCWDALRWVATHVNRDGPEPWLNNHADFNRVCLAGDSAGANICNHLAVRAGSS- 187
               ++D W AL+WV TH+   G E WLN HADF+RV L+GDSAGANI +H+A+RA    
Sbjct: 122 ISVPFDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKEK 181

Query: 188 -AEQLGGIKVVAMALIHPFFG-----DGGD--DRLWKYLCSDQTRSARPRTE-------- 247
            +  L    +  + L+HP+F      D  D  D   +         A P ++        
Sbjct: 182 LSPGLNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDPLL 241

Query: 248 --------DLAKLPCGRVKVFLAEEDFLKVGGRNYGEDLKRSRWNGAVETVEHDGEDHVF 302
                   DL+ L CG+V V +AE+D L   G  Y   L++S W G VE VE +GEDHVF
Sbjct: 242 NVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDHVF 301

[Close]

BLAST of Lsi10G002920 vs. TAIR10
Match: AT1G19190.1 (AT1G19190.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 232.3 bits (591), Expect = 4.1e-61
Identity = 120/314 (38.22%), Postives = 178/314 (56.69%), Query Frame = 1


Query: 8   NDDVAYDFFPFVRIYKDGRVQRLQITSYIVSADADDPKSAFLSKDVTISADPAVSARIFI 67
           + ++A+D+ P  RI+K+G ++RL   +++    + +P++  +SKD   S +  +S RI++
Sbjct: 2   DSEIAFDYSPRFRIFKNGGIERLVPETFV--PPSLNPENGVVSKDAVYSPEKNLSLRIYL 61

Query: 68  PS---FADPNQKLPILLYVHGGGFCIESAFNLQYHSYVGSLAVKANVVAVSVEYRLAPEH 127
           P    +    +K+P+L+Y HGGGF +E+AF+  YH+++ S     + +AVSVEYR APEH
Sbjct: 62  PQNSVYETGEKKIPLLVYFHGGGFIMETAFSPIYHTFLTSAVSATDCIAVSVEYRRAPEH 121

Query: 128 LFPACYEDCWDALRWVATHVNRDGPEPWLNNHADFNRVCLAGDSAGANICNHLAVRAGSS 187
             P  YED WDA++W+ TH+ R GPE WLN HADF++V LAGDSAGANI +H+A+R    
Sbjct: 122 PIPTLYEDSWDAIQWIFTHITRSGPEDWLNKHADFSKVFLAGDSAGANIAHHMAIRVDKE 181

Query: 188 AEQLGGIKVVAMALIHPFFGDGG------------DDRLWKYLCSDQTRSARPR-----T 247
                  K+  M L HP+F                 +RLW+    D              
Sbjct: 182 KLPPENFKISGMILFHPYFLSKALIEEMEVEAMRYYERLWRIASPDSGNGVEDPWINVVG 241

Query: 248 EDLAKLPCGRVKVFLAEEDFLKVGGRNYGEDLKRSRWNGAVETVEHDGEDHVFHLKKPEC 302
            DL  L C RV V +A  D L  GG +Y  +L++S W G V+ +E   E HVFHL+ P+ 
Sbjct: 242 SDLTGLGCRRVLVMVAGNDVLARGGWSYVAELEKSGWIGKVKVMETKEEGHVFHLRDPDS 301

[Close]

BLAST of Lsi10G002920 vs. TAIR10
Match: AT1G49660.1 (AT1G49660.1 carboxyesterase 5)

HSP 1 Score: 231.9 bits (590), Expect = 5.3e-61
Identity = 131/320 (40.94%), Postives = 183/320 (57.19%), Query Frame = 1


Query: 10  DVAYDFFPFVRIYKDGRVQRLQITSYIVSADADDPKSAFLSKDVTISADPAVSARIFIPS 69
           ++A +F PF RIYKDGRV+RL  T  I ++   DP    +SKDV  S +  +S R+F+P 
Sbjct: 4   EIASEFLPFCRIYKDGRVERLIGTDTIPASL--DPTYDVVSKDVIYSPENNLSVRLFLPH 63

Query: 70  FADP---NQKLPILLYVHGGGFCIESAFNLQYHSYVGSLAVKANVVAVSVEYRLAPEHLF 129
            +       KLP+L+Y+HGG + IES F+  YH+Y+  +   AN +AVSV+YR APE   
Sbjct: 64  KSTKLTAGNKLPLLIYIHGGAWIIESPFSPLYHNYLTEVVKSANCLAVSVQYRRAPEDPV 123

Query: 130 PACYEDCWDALRWVATHVNRDGPEPWLNNHADFNRVCLAGDSAGANICNHLAVRAGSSAE 189
           PA YED W A++W+  H N  GP  W+N HADF +V L GDSAG NI +H+A++AG   E
Sbjct: 124 PAAYEDVWSAIQWIFAHSNGSGPVDWINKHADFGKVFLGGDSAGGNISHHMAMKAGK--E 183

Query: 190 QLGGIKVVAMALIHPFFGDGGDDRLWKYLCSD-QTRS---------ARPRT--------- 249
           +   +K+  +A++HP F   G D + +Y   D +TRS         A P +         
Sbjct: 184 KKLDLKIKGIAVVHPAF--WGTDPVDEYDVQDKETRSGIAEIWEKIASPNSVNGTDDPLF 243

Query: 250 ------EDLAKLPCGRVKVFLAEEDFLKVGGRNYGEDLKRSRWNGAVETVEHDGEDHVFH 302
                  D + L C +V V +A +D     G  Y   L++  W G VE VE +GEDHVFH
Sbjct: 244 NVNGSGSDFSGLGCDKVLVAVAGKDVFVRQGLAYAAKLEKCEWEGTVEVVEEEGEDHVFH 303

[Close]

BLAST of Lsi10G002920 vs. TAIR10
Match: AT3G48700.1 (AT3G48700.1 carboxyesterase 13)

HSP 1 Score: 229.2 bits (583), Expect = 3.5e-60
Identity = 130/330 (39.39%), Postives = 191/330 (57.88%), Query Frame = 1


Query: 8   NDDVAYDFFPFVRIYKDGRVQRLQITSYIVSADADDPKSAFLSKDVTISADPAVSARIFI 67
           + ++A D+ P + IYK GR++RL   + +    + +P++  +SKDV  S D  +S RI++
Sbjct: 2   DSEIAADYSPMLIIYKSGRIERLVGETTV--PPSSNPQNGVVSKDVVYSPDNNLSLRIYL 61

Query: 68  PSFA-----DPNQKLPILLYVHGGGFCIESAFNLQYHSYVGSLAVKANVVAVSVEYRLAP 127
           P  A     + + KLP+L+Y HGGGF +E+AF+  YH+++ +    ++ VAVSV+YR AP
Sbjct: 62  PEKAATAETEASVKLPLLVYFHGGGFLVETAFSPTYHTFLTAAVSASDCVAVSVDYRRAP 121

Query: 128 EHLFPACYEDCWDALRWVATHVNRDGPEPWLNNHADFNRVCLAGDSAGANICNHLAVRAG 187
           EH  P  Y+D W AL+WV +H+   G E WLN HADF++V LAGDSAGANI +H+ ++A 
Sbjct: 122 EHPIPTSYDDSWTALKWVFSHIAGSGSEDWLNKHADFSKVFLAGDSAGANITHHMTMKAA 181

Query: 188 S---SAEQLGGIKVVAMALIHPFFGDGG--DDR-------------LWKYLCSDQTRS-- 247
               S E L    +  + L+HP+F      DD+             +W  L S  ++   
Sbjct: 182 KDKLSPESLNESGISGIILVHPYFWSKTPVDDKETTDVAIRTWIESVWT-LASPNSKDGS 241

Query: 248 -------ARPRTEDLAKLPCGRVKVFLAEEDFLKVGGRNYGEDLKRSRWNGAV-ETVEHD 305
                   +  + DL+ L CG+V V +AE+D L   G  Y E L +SRWNG V + VE  
Sbjct: 242 DDPFINVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYWEKLGKSRWNGEVLDVVETK 301

[Close]

BLAST of Lsi10G002920 vs. TAIR10
Match: AT2G03550.1 (AT2G03550.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 222.2 bits (565), Expect = 4.2e-58
Identity = 123/312 (39.42%), Postives = 172/312 (55.13%), Query Frame = 1


Query: 11  VAYDFFPFVRIYKDGRVQRLQITSYIVSADADDPKSAFLSKDVTISADPAVSARIFIPSF 70
           +A+D  P  R+YK GR++RL   + +  +    P++  +SKD+  S +  +S RI++P  
Sbjct: 5   IAFDRSPMFRVYKSGRIERLLGETTVPPSLT--PQNGVVSKDIIHSPEKNLSLRIYLPEK 64

Query: 71  ADPNQKLPILLYVHGGGFCIESAFNLQYHSYVGSLAVKANVVAVSVEYRLAPEHLFPACY 130
               +KLPIL+Y HGGGF IE+AF+  YH+++ S    AN +A+SV YR APE   P  Y
Sbjct: 65  VTV-KKLPILIYFHGGGFIIETAFSPPYHTFLTSAVAAANCLAISVNYRRAPEFPVPIPY 124

Query: 131 EDCWDALRWVATHVNRDGPEPWLNNHADFNRVCLAGDSAGANICNHLAVRAGSSAEQLGG 190
           ED WD+L+WV TH+   GPE W+N H DF +V LAGDSAG NI +HL +RA    E+L  
Sbjct: 125 EDSWDSLKWVLTHITGTGPETWINKHGDFGKVFLAGDSAGGNISHHLTMRA--KKEKLCD 184

Query: 191 IKVVAMALIHPFFGD---------------GGDDRLWKYLCSDQTRSA-----RPRTEDL 250
             +  + LIHP+F                  G +  W+    +  +            D 
Sbjct: 185 SLISGIILIHPYFWSKTPIDEFEVRDVGKTKGVEGSWRVASPNSKQGVDDPWLNVVGSDP 244

Query: 251 AKLPCGRVKVFLAEEDFLKVGGRNYGEDLKRSRWNGAVETVEHDGEDHVFHLKKPECEKA 303
           + L CGRV V +A +D     G  Y E LK+S W G VE +E   E HVFHLK P  + A
Sbjct: 245 SGLGCGRVLVMVAGDDLFVRQGWCYAEKLKKSGWEGEVEVMETKNEGHVFHLKNPNSDNA 304

[Close]

BLAST of Lsi10G002920 vs. NCBI nr
Match: gi|778669131|ref|XP_004145358.2| (PREDICTED: 2-hydroxyisoflavanone dehydratase-like [Cucumis sativus])

HSP 1 Score: 514.2 bits (1323), Expect = 1.5e-142
Identity = 246/304 (80.92%), Postives = 266/304 (87.50%), Query Frame = 1


Query: 1   MASSPNKNDDVAYDFFPFVRIYKDGRVQRLQITSYIVSADADDPKSAFLSKDVTISADPA 60
           MASS NKNDDVA+DFFPF+R+Y DGRVQRL  TS IV ADADDPKS F SKDVTIS DPA
Sbjct: 1   MASSTNKNDDVAFDFFPFLRVYTDGRVQRLMTTSDIVPADADDPKSPFRSKDVTISTDPA 60

Query: 61  VSARIFIPSFADPNQKLPILLYVHGGGFCIESAFNLQYHSYVGSLAVKANVVAVSVEYRL 120
           VSAR+FIPS ADPNQKLP+LLYVHGG FCIESAF+LQYH +VGSLA KAN VAVSVEYRL
Sbjct: 61  VSARVFIPSSADPNQKLPLLLYVHGGAFCIESAFSLQYHQHVGSLAAKANAVAVSVEYRL 120

Query: 121 APEHLFPACYEDCWDALRWVATHVNRDGPEPWLNNHADFNRVCLAGDSAGANICNHLAVR 180
           APEH  PACYEDCWDALRWVA HVNRDG EPWLN + DFNR+CLAGDSAGANIC++LA R
Sbjct: 121 APEHPIPACYEDCWDALRWVAAHVNRDGSEPWLNTYVDFNRICLAGDSAGANICHYLAAR 180

Query: 181 AGSSAEQLGGIKVVAMALIHPFFGDGGDDRLWKYLCSDQTRSARPRTEDLAKLPCGRVKV 240
           A SSAE+LGG KVVAMALIHPFFGDGG++RLWKYLCS +T+  RP  EDLAKL C RVK+
Sbjct: 181 ASSSAEELGGAKVVAMALIHPFFGDGGENRLWKYLCS-ETKLLRPTIEDLAKLGCKRVKI 240

Query: 241 FLAEEDFLKVGGRNYGEDLKRSRWNGAVETVEHDGEDHVFHLKKPECEKALDLLEKLASF 300
           FLAE DFLK GG+NY EDLK S WNG VETVEH  E+HVFHLKKPECEKA+DLLEKLASF
Sbjct: 241 FLAENDFLKSGGKNYEEDLKSSGWNGTVETVEHGEENHVFHLKKPECEKAVDLLEKLASF 300

Query: 301 INLD 305
           INLD
Sbjct: 301 INLD 303

[Close]

BLAST of Lsi10G002920 vs. NCBI nr
Match: gi|659118613|ref|XP_008459211.1| (PREDICTED: 2-hydroxyisoflavanone dehydratase-like [Cucumis melo])

HSP 1 Score: 503.1 bits (1294), Expect = 3.5e-139
Identity = 239/304 (78.62%), Postives = 263/304 (86.51%), Query Frame = 1


Query: 1   MASSPNKNDDVAYDFFPFVRIYKDGRVQRLQITSYIVSADADDPKSAFLSKDVTISADPA 60
           MASS NKN++VA+D FPF+R+Y DGRVQRL ITS  V ADADDPKS F SKDVTIS DPA
Sbjct: 1   MASSTNKNNEVAFDLFPFLRVYSDGRVQRLMITSDFVPADADDPKSPFRSKDVTISTDPA 60

Query: 61  VSARIFIPSFADPNQKLPILLYVHGGGFCIESAFNLQYHSYVGSLAVKANVVAVSVEYRL 120
           VSAR+FIPS ADPNQKLP+LLYVHGG FCIESAFNLQYH +VG+LA +AN VAVSVEYRL
Sbjct: 61  VSARVFIPSSADPNQKLPLLLYVHGGAFCIESAFNLQYHQHVGALAAEANAVAVSVEYRL 120

Query: 121 APEHLFPACYEDCWDALRWVATHVNRDGPEPWLNNHADFNRVCLAGDSAGANICNHLAVR 180
           APEH  PACY+DCWDALRWVA HVNRDG EPWLN + DFNR+CLAGDSAGANIC++LA R
Sbjct: 121 APEHPIPACYDDCWDALRWVAAHVNRDGLEPWLNTYVDFNRICLAGDSAGANICHYLAAR 180

Query: 181 AGSSAEQLGGIKVVAMALIHPFFGDGGDDRLWKYLCSDQTRSARPRTEDLAKLPCGRVKV 240
           A SS+E+LGG KVVAMALIHPFFGDG +DRLWKYLCS +TR  RP  E+LAK+ C RVK+
Sbjct: 181 ASSSSEELGGAKVVAMALIHPFFGDGREDRLWKYLCS-ETRLVRPTKEELAKMGCKRVKI 240

Query: 241 FLAEEDFLKVGGRNYGEDLKRSRWNGAVETVEHDGEDHVFHLKKPECEKALDLLEKLASF 300
           FLAE DFLK GGRNY EDLKRS WNG VETVEH GE+HVFHLK PE E A+DLLEKLASF
Sbjct: 241 FLAENDFLKFGGRNYDEDLKRSGWNGVVETVEHGGENHVFHLKNPESENAVDLLEKLASF 300

Query: 301 INLD 305
           INLD
Sbjct: 301 INLD 303

[Close]

BLAST of Lsi10G002920 vs. NCBI nr
Match: gi|470117383|ref|XP_004294838.1| (PREDICTED: 2-hydroxyisoflavanone dehydratase-like [Fragaria vesca subsp. vesca])

HSP 1 Score: 336.7 bits (862), Expect = 4.4e-89
Identity = 163/303 (53.80%), Postives = 208/303 (68.65%), Query Frame = 1


Query: 5   PNKNDDVAYDFFPFVRIYKDGRVQRLQITSYIVSADADDPKSAFLSKDVTISADPAVSAR 64
           P   DDV + FFPF ++YKDGRV+R   +        DDP S   S DV IS +P VSAR
Sbjct: 6   PPITDDVEHQFFPFFKVYKDGRVERFMPSEK--QPPCDDPSSTVRSNDVIISTEPVVSAR 65

Query: 65  IFIPSFAD-PNQKLPILLYVHGGGFCIESAFNLQYHSYVGSLAVKANVVAVSVEYRLAPE 124
           +F+P   + P +KLP+++Y+HGGGF I SAF+  YH YV SL  +ANV+AVSV+YRLAPE
Sbjct: 66  VFMPRLPNNPEKKLPVVVYIHGGGFSIASAFHPGYHKYVSSLVAQANVIAVSVDYRLAPE 125

Query: 125 HLFPACYEDCWDALRWVATHVNRDGPEPWLNNHADFNRVCLAGDSAGANICNHLAVRAGS 184
           H  PACYED W  L WVA+H N DGP+PWLN+HADF RV LAGDSAGANI +++  R GS
Sbjct: 126 HPIPACYEDSWAVLNWVASHANGDGPDPWLNSHADFGRVFLAGDSAGANISHNIVSRVGS 185

Query: 185 SAEQLGGIKVVAMALIHPFFGDGGDDRLWKYLCSD----QTRSARPRTEDLAKLPCGRVK 244
           +   L G+K+V +AL+HP+FG   DD++W Y+C +         +P  EDL +L C RV 
Sbjct: 186 TG--LPGVKIVGVALVHPYFGGTEDDKMWLYMCPENGGLDDPRLKPAVEDLRRLGCERVL 245

Query: 245 VFLAEEDFLKVGGRNYGEDLKRSRWNGAVETVEHDGEDHVFHLKKPECEKALDLLEKLAS 303
           VF+A +D L+  G +Y E LK S W G VE VEH+ E+HVFHL KPE E A+DL+ +L S
Sbjct: 246 VFVAGKDHLRERGTSYVEALKGSGWKGTVEIVEHEEENHVFHLMKPESENAVDLMSRLVS 304

[Close]

BLAST of Lsi10G002920 vs. NCBI nr
Match: gi|225428751|ref|XP_002285042.1| (PREDICTED: probable carboxylesterase 5 [Vitis vinifera])

HSP 1 Score: 336.3 bits (861), Expect = 5.7e-89
Identity = 160/305 (52.46%), Postives = 213/305 (69.84%), Query Frame = 1


Query: 2   ASSPNKNDDVAYDFFPFVRIYKDGRVQRLQITSYIVSADADDPKSAFLSKDVTISADPAV 61
           A     N  +++ FFPF +IY+DGRV+R   T ++    +DDP +   SKDV IS +  V
Sbjct: 11  AKLEKSNGQLSHLFFPFFKIYQDGRVERFMHTDHV--PPSDDPLTGVRSKDVIISPETGV 70

Query: 62  SARIFIPSFADPNQKLPILLYVHGGGFCIESAFNLQYHSYVGSLAVKANVVAVSVEYRLA 121
           SAR+FIP   +PN KLP+L+Y+HGGGF I+SAF+  Y+ YV SL  +ANV+A+SV+YRLA
Sbjct: 71  SARLFIPKLPNPNCKLPLLIYIHGGGFSIQSAFSTSYNHYVKSLVAEANVIALSVDYRLA 130

Query: 122 PEHLFPACYEDCWDALRWVATHVNRDGPEPWLNNHADFNRVCLAGDSAGANICNHLAVRA 181
           PEH  PACY+D W A++W A+H N DGP+ WLNNHADF+RV  AGDSAG NI N LA R 
Sbjct: 131 PEHPIPACYDDSWAAVQWAASHANGDGPDTWLNNHADFSRVFFAGDSAGGNISNTLAFRV 190

Query: 182 GSSAEQLGGIKVVAMALIHPFFGDGGDDRLWKYLCSD----QTRSARPRTEDLAKLPCGR 241
           GSS   L G+KVV + L+HP+FG  GDD++W Y+C +    +    +P  EDLA+L C R
Sbjct: 191 GSSG--LPGVKVVGVVLVHPYFGGTGDDQMWLYMCPNHGGLEDPRLKPGAEDLARLGCER 250

Query: 242 VKVFLAEEDFLKVGGRNYGEDLKRSRWNGAVETVEHDGEDHVFHLKKPECEKALDLLEKL 301
           V +F+AE+D L+    +Y E LK+S W G VE VE+ GE+HVFHL  P+CE A  L++K+
Sbjct: 251 VLMFVAEKDHLRPVAWDYYEKLKKSEWKGTVEIVENHGEEHVFHLMNPKCENAAVLMKKI 310

Query: 302 ASFIN 303
            SF+N
Sbjct: 311 VSFLN 311

[Close]

BLAST of Lsi10G002920 vs. NCBI nr
Match: gi|158564570|gb|ABW74473.1| (CXE carboxylesterase [Paeonia suffruticosa])

HSP 1 Score: 334.3 bits (856), Expect = 2.2e-88
Identity = 160/297 (53.87%), Postives = 208/297 (70.03%), Query Frame = 1


Query: 9   DDVAYDFFPFVRIYKDGRVQRLQITSYIVSADADDPKSAFLSKDVTISADPAVSARIFIP 68
           DDVA++FFPF R+YKDGRV+R   T  +     DDP +   SKDV IS  P V+ RIF+P
Sbjct: 33  DDVAHEFFPFFRVYKDGRVERFMPTEKV--PPTDDPNTGVRSKDVQIS--PEVAVRIFLP 92

Query: 69  SFADPNQKLPILLYVHGGGFCIESAFNLQYHSYVGSLAVKANVVAVSVEYRLAPEHLFPA 128
              DP QK+P+L Y HGGGF I SAF   YH+YV SL  +ANV+AVSV+YRLAPEH  PA
Sbjct: 93  KIDDPTQKVPVLFYTHGGGFSIGSAFAQGYHNYVSSLVAEANVIAVSVDYRLAPEHPIPA 152

Query: 129 CYEDCWDALRWVATHVNRDGPEPWLNNHADFNRVCLAGDSAGANICNHLAVRAGSSAEQL 188
           CYED W+A +WVA+H N +GPEPWLN+HADF RV + GDSAGANI + LA R GS+  +L
Sbjct: 153 CYEDSWEAFKWVASHANGNGPEPWLNDHADFRRVFMTGDSAGANITHTLAARIGST--EL 212

Query: 189 GGIKVVAMALIHPFFGDGGDDRLWKYLCSD----QTRSARPRTEDLAKLPCGRVKVFLAE 248
            G+KV+ +AL+HP+FG   DD++W +LC      +    +P TEDLAKL C ++ +F+A+
Sbjct: 213 PGVKVIGIALVHPYFGGTDDDKMWLFLCPTNGGLEDPRLKPATEDLAKLGCEKMLIFVAD 272

Query: 249 EDFLKVGGRNYGEDLKRSRWNGAVETVEHDGEDHVFHLKKPECEKALDLLEKLASFI 302
           ED LK  G +Y ++LK+S W G VE  E+ G+ HVFHL  P C+ A  + ++L SFI
Sbjct: 273 EDHLKERGISYYDELKKSGWKGTVEIEENKGQHHVFHLMNPTCDDAKAMKKRLVSFI 323

The following BLAST results are available for this feature:

BLAST of Lsi10G002920 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CXE12_ARATH	2.0e-62	40.50	Probable carboxylesterase 12 OS=Arabidopsis thaliana GN=CXE12 PE=1 SV=1	[more]
CXE1_ARATH	7.2e-60	38.22	Probable carboxylesterase 1 OS=Arabidopsis thaliana GN=CXE1 PE=2 SV=1	[more]
CXE5_ARATH	9.5e-60	40.94	Probable carboxylesterase 5 OS=Arabidopsis thaliana GN=CXE5 PE=2 SV=1	[more]
HIDM_GLYEC	1.2e-59	37.42	2-hydroxyisoflavanone dehydratase OS=Glycyrrhiza echinata GN=HIDM PE=1 SV=1	[more]
CXE13_ARATH	6.1e-59	39.39	Probable carboxylesterase 13 OS=Arabidopsis thaliana GN=CXE13 PE=1 SV=1	[more]

BLAST of Lsi10G002920 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LNZ1_CUCSA	1.1e-142	80.92	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G234530 PE=4 SV=1	[more]
F6HQD6_VITVI	4.0e-89	52.46	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_03s0063g00810 PE=4 SV=...	[more]
A8TU55_PAESU	1.5e-88	53.87	CXE carboxylesterase OS=Paeonia suffruticosa PE=2 SV=1	[more]
A0A059BCP2_EUCGR	2.2e-87	51.48	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_G01495 PE=4 SV=1	[more]
M5W4H3_PRUPE	8.6e-84	52.05	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa009174mg PE=4 SV=1	[more]

BLAST of Lsi10G002920 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G48690.1	1.1e-63	40.50	alpha/beta-Hydrolases superfamily protein	[more]
AT1G19190.1	4.1e-61	38.22	alpha/beta-Hydrolases superfamily protein	[more]
AT1G49660.1	5.3e-61	40.94	carboxyesterase 5	[more]
AT3G48700.1	3.5e-60	39.39	carboxyesterase 13	[more]
AT2G03550.1	4.2e-58	39.42	alpha/beta-Hydrolases superfamily protein	[more]

BLAST of Lsi10G002920 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778669131\|ref\|XP_004145358.2\|	1.5e-142	80.92	PREDICTED: 2-hydroxyisoflavanone dehydratase-like [Cucumis sativus]	[more]
gi\|659118613\|ref\|XP_008459211.1\|	3.5e-139	78.62	PREDICTED: 2-hydroxyisoflavanone dehydratase-like [Cucumis melo]	[more]
gi\|470117383\|ref\|XP_004294838.1\|	4.4e-89	53.80	PREDICTED: 2-hydroxyisoflavanone dehydratase-like [Fragaria vesca subsp. vesca]	[more]
gi\|225428751\|ref\|XP_002285042.1\|	5.7e-89	52.46	PREDICTED: probable carboxylesterase 5 [Vitis vinifera]	[more]
gi\|158564570\|gb\|ABW74473.1\|	2.2e-88	53.87	CXE carboxylesterase [Paeonia suffruticosa]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0016787	hydrolase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR013094	AB_hydrolase_3

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016787	hydrolase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi10G002920.1	Lsi10G002920.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013094	Alpha/beta hydrolase fold-3	PFAM	PF07859	Abhydrolase_3	coord: 80..281 score: 3.4
None	No IPR available	PANTHER	PTHR23024	MEMBER OF 'GDXG' FAMILY OF LIPOLYTIC ENZYMES	coord: 8..304 score: 8.9E
None	No IPR available	PANTHER	PTHR23024:SF228	SUBFAMILY NOT NAMED	coord: 8..304 score: 8.9E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi10G002920	Cla97C09G178830	Watermelon (97103) v2	lsiwmbB070
Lsi10G002920	Cp4.1LG16g02120	Cucurbita pepo (Zucchini)	cpelsiB220
Lsi10G002920	Cp4.1LG05g12180	Cucurbita pepo (Zucchini)	cpelsiB589

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi10G002920	Melon (DHL92) v3.5.1	lsimeB057
Lsi10G002920	Watermelon (Charleston Gray)	lsiwcgB034
Lsi10G002920	Watermelon (97103) v1	lsiwmB043
Lsi10G002920	Cucumber (Gy14) v2	cgyblsiB086
Lsi10G002920	Melon (DHL92) v3.6.1	lsimedB059
Lsi10G002920	Silver-seed gourd	carlsiB731
Lsi10G002920	Silver-seed gourd	carlsiB961
Lsi10G002920	Cucumber (Chinese Long) v3	cuclsiB104
Lsi10G002920	Wax gourd	lsiwgoB074
Lsi10G002920	Cucumber (Gy14) v1	cgylsiB092
Lsi10G002920	Cucurbita maxima (Rimu)	cmalsiB507
Lsi10G002920	Cucurbita maxima (Rimu)	cmalsiB548
Lsi10G002920	Cucurbita moschata (Rifu)	cmolsiB491
Lsi10G002920	Cucurbita moschata (Rifu)	cmolsiB533
Lsi10G002920	Wild cucumber (PI 183967)	cpilsiB091
Lsi10G002920	Cucumber (Chinese Long) v2	culsiB093