Lsi03G014730 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi03G014730
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Ubiquitin-protein ligase (Cullin)
Location	chr03 : 25619192 .. 25619437 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGTGCGCAGAAGAAGAGGAATTTCCAGATAGAGGCCTTTAAACACCGGGTCGTGGTGGATCCCAAATATGCTGAGAAGACGTGGAAGATTTTGGAACATGCAATTCACGAGATTTACAATCATAACGCTAGCGGCCTCAGCTTCGAAGAGCTTTACAGGTTGCTTCCGCTCCTTTTCATTTCACTGGTATACTACACCATGTTATTTCCTTCTCTCTTTGCTGCAACTATTCGATGGAACTAG

mRNA sequence

ATGAGTGCGCAGAAGAAGAGGAATTTCCAGATAGAGGCCTTTAAACACCGGGTCGTGGTGGATCCCAAATATGCTGAGAAGACGTGGAAGATTTTGGAACATGCAATTCACGAGATTTACAATCATAACGCTAGCGGCCTCAGCTTCGAAGAGCTTTACAGGTTGCTTCCGCTCCTTTTCATTTCACTGGTATACTACACCATGTTATTTCCTTCTCTCTTTGCTGCAACTATTCGATGGAACTAG

Coding sequence (CDS)

ATGAGTGCGCAGAAGAAGAGGAATTTCCAGATAGAGGCCTTTAAACACCGGGTCGTGGTGGATCCCAAATATGCTGAGAAGACGTGGAAGATTTTGGAACATGCAATTCACGAGATTTACAATCATAACGCTAGCGGCCTCAGCTTCGAAGAGCTTTACAGGTTGCTTCCGCTCCTTTTCATTTCACTGGTATACTACACCATGTTATTTCCTTCTCTCTTTGCTGCAACTATTCGATGGAACTAG

Protein sequence

MSAQKKRNFQIEAFKHRVVVDPKYAEKTWKILEHAIHEIYNHNASGLSFEELYRLLPLLFISLVYYTMLFPSLFAATIRWN

Homology

[Close]

BLAST of Lsi03G014730 vs. Swiss-Prot
Match: CUL3B_ARATH (Cullin-3B OS=Arabidopsis thaliana GN=CUL3B PE=1 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 5.5e-23
Identity = 51/54 (94.44%), Postives = 52/54 (96.30%), Query Frame = 1


Query: 1  MSAQKKRNFQIEAFKHRVVVDPKYAEKTWKILEHAIHEIYNHNASGLSFEELYR 55
          MS QKKRNFQIEAFK RVVVDPKYA+KTWKILEHAIHEIYNHNASGLSFEELYR
Sbjct: 1  MSNQKKRNFQIEAFKQRVVVDPKYADKTWKILEHAIHEIYNHNASGLSFEELYR 54

[Close]

BLAST of Lsi03G014730 vs. Swiss-Prot
Match: CUL3A_ARATH (Cullin-3A OS=Arabidopsis thaliana GN=CUL3A PE=1 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 1.1e-20
Identity = 47/54 (87.04%), Postives = 51/54 (94.44%), Query Frame = 1


Query: 1  MSAQKKRNFQIEAFKHRVVVDPKYAEKTWKILEHAIHEIYNHNASGLSFEELYR 55
          MS QKKRNFQIEAFKHRVVVDPKYA+KTW+ILE AIH+IYN +ASGLSFEELYR
Sbjct: 1  MSNQKKRNFQIEAFKHRVVVDPKYADKTWQILERAIHQIYNQDASGLSFEELYR 54

[Close]

BLAST of Lsi03G014730 vs. Swiss-Prot
Match: CUL3_DICDI (Cullin-3 OS=Dictyostelium discoideum GN=culC PE=3 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 6.1e-06
Identity = 21/39 (53.85%), Postives = 30/39 (76.92%), Query Frame = 1


Query: 16 HRVVVDPKYAEKTWKILEHAIHEIYNHNASGLSFEELYR 55
          H V+ DP + ++TWK+L+ A+ +I+  NAS LSFEELYR
Sbjct: 17 HGVLADPDFPKRTWKLLKTAMRQIHQQNASNLSFEELYR 55

[Close]

BLAST of Lsi03G014730 vs. TrEMBL
Match: A0A0A0LCT4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G827210 PE=3 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 6.5e-23
Identity = 54/54 (100.00%), Postives = 54/54 (100.00%), Query Frame = 1


Query: 1  MSAQKKRNFQIEAFKHRVVVDPKYAEKTWKILEHAIHEIYNHNASGLSFEELYR 55
          MSAQKKRNFQIEAFKHRVVVDPKYAEKTWKILEHAIHEIYNHNASGLSFEELYR
Sbjct: 1  MSAQKKRNFQIEAFKHRVVVDPKYAEKTWKILEHAIHEIYNHNASGLSFEELYR 54

[Close]

BLAST of Lsi03G014730 vs. TrEMBL
Match: M5X9N4_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa001982mg PE=3 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 6.5e-23
Identity = 54/54 (100.00%), Postives = 54/54 (100.00%), Query Frame = 1


Query: 1  MSAQKKRNFQIEAFKHRVVVDPKYAEKTWKILEHAIHEIYNHNASGLSFEELYR 55
          MSAQKKRNFQIEAFKHRVVVDPKYAEKTWKILEHAIHEIYNHNASGLSFEELYR
Sbjct: 1  MSAQKKRNFQIEAFKHRVVVDPKYAEKTWKILEHAIHEIYNHNASGLSFEELYR 54

[Close]

BLAST of Lsi03G014730 vs. TrEMBL
Match: A0A103YHC6_CYNCS (Cullin homology OS=Cynara cardunculus var. scolymus GN=Ccrd_012504 PE=3 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 1.5e-22
Identity = 53/54 (98.15%), Postives = 54/54 (100.00%), Query Frame = 1


Query: 1  MSAQKKRNFQIEAFKHRVVVDPKYAEKTWKILEHAIHEIYNHNASGLSFEELYR 55
          MS+QKKRNFQIEAFKHRVVVDPKYAEKTWKILEHAIHEIYNHNASGLSFEELYR
Sbjct: 1  MSSQKKRNFQIEAFKHRVVVDPKYAEKTWKILEHAIHEIYNHNASGLSFEELYR 54

[Close]

BLAST of Lsi03G014730 vs. TrEMBL
Match: A5C158_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_020617 PE=3 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 1.9e-22
Identity = 53/54 (98.15%), Postives = 53/54 (98.15%), Query Frame = 1


Query: 1  MSAQKKRNFQIEAFKHRVVVDPKYAEKTWKILEHAIHEIYNHNASGLSFEELYR 55
          MS QKKRNFQIEAFKHRVVVDPKYAEKTWKILEHAIHEIYNHNASGLSFEELYR
Sbjct: 1  MSTQKKRNFQIEAFKHRVVVDPKYAEKTWKILEHAIHEIYNHNASGLSFEELYR 54

[Close]

BLAST of Lsi03G014730 vs. TrEMBL
Match: V4TPY5_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10030804mg PE=3 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 3.2e-22
Identity = 53/54 (98.15%), Postives = 53/54 (98.15%), Query Frame = 1


Query: 1  MSAQKKRNFQIEAFKHRVVVDPKYAEKTWKILEHAIHEIYNHNASGLSFEELYR 55
          MS QKKRNFQIEAFKHRVVVDPKYAEKTWKILEHAIHEIYNHNASGLSFEELYR
Sbjct: 1  MSNQKKRNFQIEAFKHRVVVDPKYAEKTWKILEHAIHEIYNHNASGLSFEELYR 54

[Close]

BLAST of Lsi03G014730 vs. TAIR10
Match: AT1G69670.1 (AT1G69670.1 cullin 3B)

HSP 1 Score: 107.8 bits (268), Expect = 3.1e-24
Identity = 51/54 (94.44%), Postives = 52/54 (96.30%), Query Frame = 1


Query: 1  MSAQKKRNFQIEAFKHRVVVDPKYAEKTWKILEHAIHEIYNHNASGLSFEELYR 55
          MS QKKRNFQIEAFK RVVVDPKYA+KTWKILEHAIHEIYNHNASGLSFEELYR
Sbjct: 1  MSNQKKRNFQIEAFKQRVVVDPKYADKTWKILEHAIHEIYNHNASGLSFEELYR 54

[Close]

BLAST of Lsi03G014730 vs. TAIR10
Match: AT1G26830.1 (AT1G26830.1 cullin 3)

HSP 1 Score: 100.1 bits (248), Expect = 6.5e-22
Identity = 47/54 (87.04%), Postives = 51/54 (94.44%), Query Frame = 1


Query: 1  MSAQKKRNFQIEAFKHRVVVDPKYAEKTWKILEHAIHEIYNHNASGLSFEELYR 55
          MS QKKRNFQIEAFKHRVVVDPKYA+KTW+ILE AIH+IYN +ASGLSFEELYR
Sbjct: 1  MSNQKKRNFQIEAFKHRVVVDPKYADKTWQILERAIHQIYNQDASGLSFEELYR 54

[Close]

BLAST of Lsi03G014730 vs. NCBI nr
Match: gi|595963642|ref|XP_007217024.1| (hypothetical protein PRUPE_ppa001982mg [Prunus persica])

HSP 1 Score: 114.4 bits (285), Expect = 9.4e-23
Identity = 54/54 (100.00%), Postives = 54/54 (100.00%), Query Frame = 1


Query: 1  MSAQKKRNFQIEAFKHRVVVDPKYAEKTWKILEHAIHEIYNHNASGLSFEELYR 55
          MSAQKKRNFQIEAFKHRVVVDPKYAEKTWKILEHAIHEIYNHNASGLSFEELYR
Sbjct: 1  MSAQKKRNFQIEAFKHRVVVDPKYAEKTWKILEHAIHEIYNHNASGLSFEELYR 54

[Close]

BLAST of Lsi03G014730 vs. NCBI nr
Match: gi|697137979|ref|XP_009623064.1| (PREDICTED: cullin-3A-like isoform X1 [Nicotiana tomentosiformis])

HSP 1 Score: 114.4 bits (285), Expect = 9.4e-23
Identity = 54/54 (100.00%), Postives = 54/54 (100.00%), Query Frame = 1


Query: 1  MSAQKKRNFQIEAFKHRVVVDPKYAEKTWKILEHAIHEIYNHNASGLSFEELYR 55
          MSAQKKRNFQIEAFKHRVVVDPKYAEKTWKILEHAIHEIYNHNASGLSFEELYR
Sbjct: 1  MSAQKKRNFQIEAFKHRVVVDPKYAEKTWKILEHAIHEIYNHNASGLSFEELYR 54

[Close]

BLAST of Lsi03G014730 vs. NCBI nr
Match: gi|659085437|ref|XP_008443419.1| (PREDICTED: cullin-3A-like [Cucumis melo])

HSP 1 Score: 114.4 bits (285), Expect = 9.4e-23
Identity = 54/54 (100.00%), Postives = 54/54 (100.00%), Query Frame = 1


Query: 1  MSAQKKRNFQIEAFKHRVVVDPKYAEKTWKILEHAIHEIYNHNASGLSFEELYR 55
          MSAQKKRNFQIEAFKHRVVVDPKYAEKTWKILEHAIHEIYNHNASGLSFEELYR
Sbjct: 1  MSAQKKRNFQIEAFKHRVVVDPKYAEKTWKILEHAIHEIYNHNASGLSFEELYR 54

[Close]

BLAST of Lsi03G014730 vs. NCBI nr
Match: gi|449459468|ref|XP_004147468.1| (PREDICTED: cullin-3A [Cucumis sativus])

HSP 1 Score: 114.4 bits (285), Expect = 9.4e-23
Identity = 54/54 (100.00%), Postives = 54/54 (100.00%), Query Frame = 1


Query: 1  MSAQKKRNFQIEAFKHRVVVDPKYAEKTWKILEHAIHEIYNHNASGLSFEELYR 55
          MSAQKKRNFQIEAFKHRVVVDPKYAEKTWKILEHAIHEIYNHNASGLSFEELYR
Sbjct: 1  MSAQKKRNFQIEAFKHRVVVDPKYAEKTWKILEHAIHEIYNHNASGLSFEELYR 54

[Close]

BLAST of Lsi03G014730 vs. NCBI nr
Match: gi|698475882|ref|XP_009785295.1| (PREDICTED: cullin-3A-like isoform X1 [Nicotiana sylvestris])

HSP 1 Score: 114.4 bits (285), Expect = 9.4e-23
Identity = 54/54 (100.00%), Postives = 54/54 (100.00%), Query Frame = 1


Query: 1  MSAQKKRNFQIEAFKHRVVVDPKYAEKTWKILEHAIHEIYNHNASGLSFEELYR 55
          MSAQKKRNFQIEAFKHRVVVDPKYAEKTWKILEHAIHEIYNHNASGLSFEELYR
Sbjct: 1  MSAQKKRNFQIEAFKHRVVVDPKYAEKTWKILEHAIHEIYNHNASGLSFEELYR 54

The following BLAST results are available for this feature:

BLAST of Lsi03G014730 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CUL3B_ARATH	5.5e-23	94.44	Cullin-3B OS=Arabidopsis thaliana GN=CUL3B PE=1 SV=1	[more]
CUL3A_ARATH	1.1e-20	87.04	Cullin-3A OS=Arabidopsis thaliana GN=CUL3A PE=1 SV=1	[more]
CUL3_DICDI	6.1e-06	53.85	Cullin-3 OS=Dictyostelium discoideum GN=culC PE=3 SV=1	[more]

BLAST of Lsi03G014730 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LCT4_CUCSA	6.5e-23	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G827210 PE=3 SV=1	[more]
M5X9N4_PRUPE	6.5e-23	100.00	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa001982mg PE=3 SV=1	[more]
A0A103YHC6_CYNCS	1.5e-22	98.15	Cullin homology OS=Cynara cardunculus var. scolymus GN=Ccrd_012504 PE=3 SV=1	[more]
A5C158_VITVI	1.9e-22	98.15	Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_020617 PE=3 SV=1	[more]
V4TPY5_9ROSI	3.2e-22	98.15	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10030804mg PE=3 SV=1	[more]

BLAST of Lsi03G014730 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G69670.1	3.1e-24	94.44	cullin 3B	[more]
AT1G26830.1	6.5e-22	87.04	cullin 3	[more]

BLAST of Lsi03G014730 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|595963642\|ref\|XP_007217024.1\|	9.4e-23	100.00	hypothetical protein PRUPE_ppa001982mg [Prunus persica]	[more]
gi\|697137979\|ref\|XP_009623064.1\|	9.4e-23	100.00	PREDICTED: cullin-3A-like isoform X1 [Nicotiana tomentosiformis]	[more]
gi\|659085437\|ref\|XP_008443419.1\|	9.4e-23	100.00	PREDICTED: cullin-3A-like [Cucumis melo]	[more]
gi\|449459468\|ref\|XP_004147468.1\|	9.4e-23	100.00	PREDICTED: cullin-3A [Cucumis sativus]	[more]
gi\|698475882\|ref\|XP_009785295.1\|	9.4e-23	100.00	PREDICTED: cullin-3A-like isoform X1 [Nicotiana sylvestris]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR016159	Cullin_repeat-like_dom_sf

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0031461	cullin-RING ubiquitin ligase complex
molecular_function	GO:0031625	ubiquitin protein ligase binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G014730.1	Lsi03G014730.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016159	Cullin repeat-like-containing domain	unknown	SSF74788	Cullin repeat-like	coord: 22..56 score: 7.5
None	No IPR available	PANTHER	PTHR11932	CULLIN	coord: 1..54 score: 1.5
None	No IPR available	PANTHER	PTHR11932:SF86	SUBFAMILY NOT NAMED	coord: 1..54 score: 1.5

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi03G014730	Csa3G827210	Cucumber (Chinese Long) v2	culsiB186
Lsi03G014730	CSPI03G39090	Wild cucumber (PI 183967)	cpilsiB184
Lsi03G014730	Cucsa.338990	Cucumber (Gy14) v1	cgylsiB551
Lsi03G014730	CmaCh11G010300	Cucurbita maxima (Rimu)	cmalsiB119
Lsi03G014730	CmaCh14G005980	Cucurbita maxima (Rimu)	cmalsiB242
Lsi03G014730	CmoCh11G010510	Cucurbita moschata (Rifu)	cmolsiB107
Lsi03G014730	CmoCh14G006050	Cucurbita moschata (Rifu)	cmolsiB230
Lsi03G014730	MELO3C009879	Melon (DHL92) v3.5.1	lsimeB260
Lsi03G014730	ClCG10G014970	Watermelon (Charleston Gray)	lsiwcgB219
Lsi03G014730	MELO3C009879.2	Melon (DHL92) v3.6.1	lsimedB273
Lsi03G014730	Cla97C10G197840	Watermelon (97103) v2	lsiwmbB218
Lsi03G014730	Bhi11G002507	Wax gourd	lsiwgoB300
Lsi03G014730	Cp4.1LG04g01390	Cucurbita pepo (Zucchini)	cpelsiB547
Lsi03G014730	CsGy3G036680	Cucumber (Gy14) v2	cgyblsiB171
Lsi03G014730	Carg22897	Silver-seed gourd	carlsiB191
Lsi03G014730	Carg09781	Silver-seed gourd	carlsiB394
Lsi03G014730	CsaV3_3G041550	Cucumber (Chinese Long) v3	cuclsiB203

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi03G014730	Cucumber (Chinese Long) v3	cuclsiB381
Lsi03G014730	Cucumber (Chinese Long) v3	cuclsiB383
Lsi03G014730	Watermelon (97103) v2	lsiwmbB252
Lsi03G014730	Wax gourd	lsiwgoB313
Lsi03G014730	Wax gourd	lsiwgoB340
Lsi03G014730	Bottle gourd (USVL1VR-Ls)	lsilsiB094
Lsi03G014730	Cucumber (Gy14) v1	cgylsiB255
Lsi03G014730	Cucumber (Gy14) v1	cgylsiB463
Lsi03G014730	Cucurbita maxima (Rimu)	cmalsiB658
Lsi03G014730	Cucurbita moschata (Rifu)	cmolsiB647
Lsi03G014730	Cucurbita pepo (Zucchini)	cpelsiB145
Lsi03G014730	Cucurbita pepo (Zucchini)	cpelsiB503
Lsi03G014730	Wild cucumber (PI 183967)	cpilsiB359
Lsi03G014730	Wild cucumber (PI 183967)	cpilsiB360
Lsi03G014730	Watermelon (Charleston Gray)	lsiwcgB244
Lsi03G014730	Cucumber (Chinese Long) v2	culsiB356
Lsi03G014730	Cucumber (Chinese Long) v2	culsiB355
Lsi03G014730	Melon (DHL92) v3.5.1	lsimeB237
Lsi03G014730	Watermelon (Charleston Gray)	lsiwcgB216
Lsi03G014730	Watermelon (97103) v1	lsiwmB236
Lsi03G014730	Watermelon (97103) v1	lsiwmB240
Lsi03G014730	Watermelon (97103) v1	lsiwmB257
Lsi03G014730	Cucumber (Gy14) v2	cgyblsiB328
Lsi03G014730	Melon (DHL92) v3.6.1	lsimedB245
Lsi03G014730	Melon (DHL92) v3.6.1	lsimedB250
Lsi03G014730	Silver-seed gourd	carlsiB238